Acipenser caviar extract composition as well as preparation method and application thereof

Abstract

The invention provides a sturgeon caviar extract composition as well as a preparation method and application thereof. Belongs to the field of medical, dental or cosmetic preparations. The composition formula comprises a galactose yeast-like bacterium fermentation product, a sturgeon caviar extract, a cynanchum atratum extract, a dendrobium officinale extract, oat peptide and an eucalyptus globulus leaf extract. The composition provided by the invention combines a plurality of active components, and discusses and studies the synergistic benefits among the components; the composition has good effects in multiple directions of moisturizing, repairing, anti-aging, soothing, anti-inflammation and the like on the skin, and improves the state of the skin. The composition provided by the invention can be added into various different cosmetics and is wide in application.

Description

Technical Field

[0001] The invention belongs to the field of medical, dental or cosmetic preparations, and in particular relates to a sturgeon caviar extract composition and a preparation method and application thereof. Background Art

[0002] Caviar extract is rich in protein and fatty acids, and contains a variety of vitamins, minerals and other nutrients. The proteins are mainly egg yolk phospholipids, egg yolk phospholipids, egg yolk globulins, etc., which can provide moisturizing, soothing, firming and anti-oxidation effects to the skin. Lipids and amino acids are one of the components that cannot be obtained by the skin barrier. As for vitamins E and B, they are both well-known and excellent ingredients currently active in skin conditioning. Therefore, in cosmetics, the main function of caviar extract is to delay skin aging.

[0003] However, the research on sturgeon caviar extract is still in a relatively blank stage. Most of the research directions are focused on the cultivation of sturgeon roe. The research on human care remains at the basic level. The unique protein and peptide substances' mechanism of action and synergistic effect need further study. In particular, the combination of sturgeon caviar extract with other functions has a very large room for development. Summary of the invention

[0004] The purpose of the present invention is to propose a sturgeon caviar extract composition and a preparation method and application thereof, mainly to expand and explore the potential of sturgeon caviar extract in human body care. The present invention finds that after the sturgeon caviar extract is used in combination with a galactosaccharomyces-like bacteria fermentation product, a cynanchum atratum extract, a dendrobium officinale extract, an oat peptide and an eucalyptus globulus leaf extract, the combination has excellent effects in the direction of anti-skin aging and moisturizing functions.

[0005] In order to achieve the above object, the present invention provides a sturgeon caviar extract composition, which comprises the following components in parts by mass:

[0006]

[0007]

[0008] In particular, the components include:

[0009] Butanediol 60-74.4 parts.

[0010] In particular, the components include:

[0011] 1,2-Hexanediol 0.2-1.0 part

[0012] In particular, the components include:

[0013] Caprylhydroxamic acid 0.05-0.1 parts.

[0014] The present invention also provides a method for preparing the aforementioned sturgeon caviar extract composition, comprising the following steps:

[0015] (1) Add all components and water into a container and heat to 80-85°C to mix;

[0016] (2) Maintain the mixture until it is completely dissolved and cool to a maximum of 40°C;

[0017] (3) Then, the heating is stopped and the mixture is allowed to cool to room temperature to obtain a sturgeon caviar extract composition.

[0018] In particular, after the temperature is lowered to a maximum of 40° C. in step (2), the auxiliary agent is added.

[0019] The auxiliary agent is at least one of the following ingredients: preservatives, antioxidants, stabilizers, moisturizers, anti-allergic agents, pH regulators, metal chelators, emollients, skin conditioners, and emulsifiers.

[0020] The third aspect of the present invention provides use of the aforementioned sturgeon caviar extract composition in the preparation of cosmetics.

[0021] The cosmetics are cosmetics for hair and / or scalp, including but not limited to: shampoo, conditioner, hair mask, scalp essential oil,

[0022] The cosmetics are cosmetics used on the surface of human skin, including but not limited to: lotion, cream, mask, essence oil, body lotion, etc.

[0023] In the present invention:

[0024] Galactomyces Ferment: Helps improve skin barrier function and enhance skin's ability to retain moisture, and may have antioxidant and anti-inflammatory effects.

[0025] Caviar Extract: Rich in protein, amino acids and vitamins, it has a nourishing and repairing effect. It can improve the elasticity and firmness of the skin, reduce the appearance of fine lines and wrinkles, and has antioxidant and anti-aging effects.

[0026] Cynanchum atratum Extract: Has moisturizing and antioxidant properties. It can help protect the skin from environmental damage.

[0027] Butylene Glycol: Humectant.

[0028] 1,2-Hexanediol: Humectant and preservative.

[0029] Caprylhydroxamic acid: acts as a chelating agent and preservative.

[0030] Dendrobium officinale extract: rich in Dendrobium officinale polysaccharides, it has moisturizing and antioxidant effects.

[0031] Oatmeal (AVENA SATIVA) peptide: Oatmeal peptide is a small molecule bioactive peptide extracted from oats, which can effectively remove free radicals and achieve antioxidant, anti-aging and redness-reducing effects on the skin.

[0032] Eucalyptus globulus leaf extract: has antibacterial and anti-inflammatory properties, helps the skin resist external environmental damage, reduces skin inflammation and acne, and may have moisturizing and soothing effects on the skin.

[0033] The beneficial technical effects of the present invention are at least as follows:

[0034] (1) The composition provided by the present invention combines multiple active ingredients, explores and studies the synergistic effects between the components; it has good effects in multiple directions such as moisturizing, repairing, anti-aging, soothing and anti-inflammatory, and improves skin condition.

[0035] (2) The composition provided by the present invention can be added to various cosmetics and has a wide range of uses. DETAILED DESCRIPTION

[0036] In order to better understand the present invention, the present invention is further described below in conjunction with specific serial numbers, wherein the terms used in the serial numbers are for describing specific embodiments and do not constitute limitations on the scope of protection of the present invention.

[0037] Specific implementation methods, unless otherwise specified, are conventional experimental methods, and the materials, reagents, etc. used, unless otherwise specified, can be obtained from commercial channels.

[0038] The sources of some raw materials used in the present invention are as follows: Table 1:

[0039] Table 1

[0040]

[0041]

[0042] The preparation of the composition comprises the following steps:

[0043] (1) Take a stirring pot as a container, add an appropriate amount of water, then put some components into the stirring pot, turn on the stirring function and heat it to 80°C, and maintain the temperature in the pot at 82.5±2.5°C; until all components are dissolved and mixed into a uniform state;

[0044] (2) Cool down to 40°C, keep stirring, and add the remaining components until the material is mixed into a uniform state;

[0045] (3) Stop heating and let it cool to room temperature.

[0046] The specific ratios are shown in Tables 2-1 and 2-2 below.

[0047] Table 2-1 Preparation ratio of the composition

[0048] Raw material / serial number 1 2 3 4 5 6 Galacto-saccharomyces cerevisiae fermentation products 6.4 10.3 6.1 - 17 6.8 Sturgeon Caviar Extract 10 9 8 16 - 10 DNA sodium - - - - - - White Rose Extract 1.0 0.6 0.3 1.0 - 1.0 Dendrobium officinale extract 0.8 1.5 2.0 0.8 0.8 - Dendrobium officinale extract - - - - - - Oat peptide 0.6 1.0 0.6 0.6 0.6 0.6 Oat Kernel Protein - - - - - - Eucalyptus globulus leaf extract 0.1 0.2 0.2 0.1 0.1 0.1 Lemon Eucalyptus Extract - - - - - - Butanediol 74.4 60.0 65.0 74.4 74.4 74.4 1,2-Hexanediol 1.0 0.2 0.6 1.0 1.0 1.0 Caprylhydroxamic acid 0.05 0.10 0.10 0.05 0.05 0.05 water Up to 100 Up to 100 Up to 100 Up to 100 Up to 100 Up to 100

[0049] Table 2-2 Preparation ratio of the composition

[0050]

[0051]

[0052] Note: "-" in Table 2-1 and Table 2-2 means no addition, and the units are all parts by mass. Among them, 1,2-hexanediol and octanoyl hydroxamic acid are added in step (2), and the remaining components are added in step (1).

[0053] Sample stability test:

[0054] 1. Heat resistance test: The constant temperature incubator was adjusted to 40°C. Three of each of the 12 samples prepared above were put into transparent glass bottles with a sample volume of 20 ml / bottle. After sealing, the bottles were placed in a constant temperature incubator. After three months, the bottles were taken out and returned to room temperature to observe changes in appearance.

[0055] 2. Cold resistance test: The constant temperature incubator was adjusted to -10℃. Three of each of the 12 samples prepared above were placed in transparent glass bottles with a sample volume of 20ml / bottle. After sealing, the bottles were placed in a constant temperature incubator. After three months, the bottles were taken out and returned to room temperature to observe changes in appearance.

[0056] 3. Room temperature test: Take three of each of the 12 samples prepared above and put them into transparent glass bottles with a sample volume of 20 ml / bottle. After sealing, place them at room temperature for 1 month and observe the changes in the appearance of the samples.

[0057] No precipitation, settling, cracking, water loss, or breakage was observed in the heat resistance test, cold resistance test, and room temperature test, and the original appearance was maintained.

[0058] Human skin patch test (4-fold dilution)

[0059] The 12 groups of serial numbers 1-12 were diluted to 20% concentration by adding 4 times the volume of water; refer to the human skin patch test in the "2022 Cosmetic Safety Technical Specifications". Observe the skin reaction according to the standard at 30 minutes (after the indentation disappears), 24 hours and 48 hours, and record the observation results. The reaction level after 30 minutes, 24 hours and 48 hours is 0.

[0060] Antioxidant test (10% concentration)

[0061] The DPPH methanol solution is violet in color and has a strong absorbance at 517nm. If combined with the sample, the absorbance value at 517nm will be reduced, thereby judging the ability of the sample to scavenge DPPH free radicals.

[0062] The specific method is as follows:

[0063] (1) Take an equal volume (2 mL) of the test solution diluted to 10% (with deionized water) and mix with 2 × 10 -4 mol / L DPPH solution mixed (A1);

[0064] (2) Take an equal volume of anhydrous ethanol (solvent for the test substance) and 2×10 -4 mol / L DPPH solution and mix well (A2);

[0065] (3) Take an equal volume of anhydrous ethanol and mix it with the test solution (A3);

[0066] (4) After 40 minutes of reaction, measure the absorbance of tubes A1, A2, and A3 at 517 nm.

[0067] The calculation formula of clearance rate is: Clearance rate (%) = [1-(A1-A3) / A2] × 100%

[0068] Table 3 Antioxidant test results

[0069]

[0070] From the results in Table 3, it can be seen that when the fermentation product of galactosaccharomyces cerevisiae (serial number 4), caviar extract (serial number 5), white osmanthus extract (serial number 5), Dendrobium officinale extract (serial number 6) and eucalyptus globulus leaf extract (serial number 8) are missing, the antioxidant properties are greatly reduced; while the lack of oat peptides has a lower effect on antioxidant properties. Using DNA sodium (i.e. PDRN, salmon roe or salmon sperm cell extract) to replace caviar extract (serial number 9), Dendrobium officinale (serial number 10), and lemon eucalyptus to replace eucalyptus globulus (serial number 12) will cause a certain degree of decline in antioxidant properties, which is speculated to be caused by the different components in different plant extracts or the difference in the content of antioxidant components.

[0071] Inflammatory cell inhibition rate test

[0072] Test principle: The neutrophils of zebrafish are similar to those of mammals in morphology, biochemistry and function. The soothing effect of the product is evaluated by testing the changes in the number of neutrophils in fish embryos.

[0073] Test method: Wild-type AB strain zebrafish embryos were selected and cultured at 28°C at a density of no more than one fish embryo in 200 μL fish embryo culture medium until 3 days after fertilization.

[0074] 42 fish embryos were randomly selected and divided into 14 groups; one group was the injury model control group, and the remaining thirteen groups were experimental groups.

[0075] First, use the inflammatory inducer solution (weigh 159.6 mg copper sulfate (CuSO4 · 5H2O) was dissolved in 1000mL of water and diluted 10 times with fish embryo culture medium, and then exposed to 0.2ml of the culture medium of each group.

[0076] The control group of the inflammation model was fish embryo culture medium; the experimental group was the test group dissolved in the fish embryo culture medium according to proportion.

[0077] Place in a 28±1°C incubator and culture for 40-45 minutes. Transfer to a 2mL centrifuge tube, add 4% PFA (paraformaldehyde solution) solution to fix, stain, and bleach at room temperature, place under a stereo microscope, photograph the fish embryos according to uniform photographic parameters, and analyze and calculate the number of neutrophils in the fish embryo cells.

[0078] The experimental results are shown in Table 4. The inhibition rate of zebrafish embryo inflammatory cells (neutrophils) was calculated according to the following formula:

[0079] Zebrafish embryo inflammatory cell (neutrophil) inhibition rate = [(zebrafish embryo inflammation model control group inflammatory cell number - zebrafish embryo inflammation model sample test group inflammatory cell number) * 100 / zebrafish embryo inflammation model control group inflammatory cell number] %.

[0080] The soothing efficacy of the samples was evaluated based on the inhibition rate of the samples on zebrafish embryo inflammatory cells and the statistical differences in the inhibition rates.

[0081] Table 4 Inflammatory cell inhibition rate test results

[0082]

[0083] According to Table 4, the lack of either the fermentation product of galactosaccharomyces cerevisiae or the caviar extract, which is used in large amounts, will lead to a significant reduction in the anti-inflammatory effect, indicating that the two have a clear synergistic effect; and the lack of the others, such as Dendrobium officinale extract, oat peptides, and Eucalyptus globulus leaf extract, will also reduce its anti-inflammatory effect; from the results of No. 9-12, it can be seen that DNA sodium (replacing caviar extract, Dendrobium officinale replacing Dendrobium officinale, and lemon eucalyptus replacing globulus will cause a certain degree of decline in the anti-inflammatory effect, and the degree of decline will also have different results depending on the different contents.

[0084] Promotes Type I collagen test

[0085] Test principle: Elastic fibers are composed of elastin and microfibrils, distributed in the dermis and subcutaneous tissue, making the skin elastic. However, due to the effects of some environmental factors such as ultraviolet radiation, pressure, pollution, etc., the production of elastase in the body will be promoted. Elastase is a member of the chymosin family, which will degrade elastin and cause the loss of connecting tissue in the epidermis, thus leading to skin aging, wrinkles and photoaging. Porcine pancreatic elastase can be used as the research object, and N-succinyl-alanine-alanine-alanine-p-nitroaniline (AAAPVN) can be used as a substrate. Porcine pancreatic elastase can hydrolyze AAAPVN, and its hydrolysis product can cause an increase in absorbance at a wavelength of 420nm. The absorbance is measured with an enzyme marker to evaluate the firming and anti-wrinkle effect of the sample to be tested.

[0086] Test method:

[0087] 1. Solution Preparation

[0088] (1) The concentration of the samples is set as shown in Table 5.

[0089] Table 5 Test concentration setting table

[0090]

[0091] (2) Prepare Tris-HCl buffer (0.1 M pH = 8.0): weigh 2.42 g Tris into a beaker, add 200 mL ultrapure water, and adjust the pH to 8.0 with concentrated HCl after complete dissolution.

[0092] (3) Preparation of positive control tea polyphenol solution (1 mg / ml): Weigh 5 mg of tea polyphenols and dissolve it in 5 mL of Tris-HCl buffer.

[0093] (4) Preparation of substrate solution AAAPVN (2 mM): Weigh 4.51 mg of N-succinyl-alanine-alanine-alanine-p-nitroaniline and dissolve it in 5 ml of Tris-HCl buffer.

[0094] (5) Prepare porcine pancreatic elastase solution (0.171 U / mL): Dissolve 280 μl of the solution in 10 ml of Tris-HCl buffer.

[0095] 2. Sample addition

[0096] The experiment was divided into 4 groups, namely, sample group, positive control group, blank control group, and model control group. Four replicate wells were set at the same concentration in the same group. The amount of each solution added is shown in Table 6.

[0097] Table 6 Experimental groups

[0098]

[0099]

[0100] 3. Determination

[0101] The reaction was allowed to proceed at room temperature for 15 min, and the absorbance was measured at 420 nm using an enzyme-labeled instrument.

[0102] 4. Calculation of results

[0103]

[0104] Where: A0-average absorbance of blank control wells;

[0105] A1-average absorbance of sample wells;

[0106] A2 - average absorbance of model control wells.

[0107] 5. Statistics

[0108] SPSS22.0 statistical software package was used for statistical analysis, and descriptive statistics were performed on the measured values ​​of the test area. The changes in the values ​​and the differences between the control group and the sample group were calculated and analyzed. If the test data was normally distributed, the independent T test method was used for statistical analysis; if the test data was non-normally distributed, the rank sum test method was used for statistical analysis. The statistical methods all used two-tailed tests, and the test level α = 0.05.

[0109] 6. Result determination

[0110] Compared with the model control group, the absorbance of the sample group showed a significant positive difference, indicating that the tested sample had the effect of inhibiting porcine pancreatic elastase. Otherwise, it had no such effect.

[0111] 7. Experimental Results

[0112] Table 7 Results of porcine pancreatic elastase inhibition rate test

[0113]

[0114]

[0115] Compared with the model control group, the inhibition rate of tea polyphenol elastase in the positive control group was 70.08%, and its absorbance was significantly different from that in the model group, which proved that this experiment was effective.

[0116] Compared with the model control group, the elastase inhibition rates of samples 1, 2, and 3 were 82.12%, 81.67%, and 81.89%, respectively, and their absorbances were significantly different from those of the model control group (p<0.05).

[0117] However, the inhibitory effects of samples 4-12 were reduced to varying degrees due to the replacement of ingredients or the lack of some ingredients.

[0118] It can be seen from the above experimental results that the composition has an inhibitory effect on porcine pancreatic elastase and has a certain firming and anti-wrinkle effect.

[0119] The above detailed description is a specific description of one feasible embodiment of the present invention. The embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or modification that does not deviate from the present invention should be included in the scope of the technical solution of the present invention.

Claims

1. A sturgeon caviar extract composition, characterized in that The following components are included in parts by mass:

2. The sturgeon caviar extract composition according to claim 1, characterized in that Also includes components: Butanediol 60-74.4 parts.

3. The sturgeon caviar extract composition according to claim 1, characterized in that Also includes components: 1,2-Hexanediol 0.2-1.0 part 4. The sturgeon caviar extract composition according to claim 1, characterized in that Also includes components: Caprylhydroxamic acid 0.05-0.1 parts.

5. The method for preparing the sturgeon caviar extract composition according to any one of claims 1 to 4, characterized in that: The following steps are involved: (1) Add all components and water into a container and heat to 80-85°C to mix; (2) Maintain the mixture until it is completely dissolved and cool to a maximum of 40°C; (3) Then, the heating is stopped and the mixture is allowed to cool to room temperature to obtain a sturgeon caviar extract composition.

6. The method for preparing the sturgeon caviar extract composition according to claim 5, characterized in that: After the temperature is lowered to a maximum of 40° C. in step (2), an auxiliary agent is added.

7. The method for preparing the sturgeon caviar extract composition according to claim 6, characterized in that: The auxiliary agent is at least one of the following ingredients: preservatives, antioxidants, stabilizers, moisturizers, anti-allergic agents, pH regulators, metal chelators, emollients, skin conditioners, and emulsifiers.

8. Use of the sturgeon caviar extract composition according to any one of claims 1 to 4 in the preparation of cosmetics.

9. Use of the sturgeon caviar extract composition according to claim 8 in preparing cosmetics, characterized in that: The cosmetic is a cosmetic for hair and / or scalp.

10. Use of the sturgeon caviar extract composition according to claim 8 in preparing cosmetics, characterized in that: The cosmetics are cosmetics used on the surface of human skin.

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