Novel extraction process of botanical fungicide allicin

Abstract

The invention discloses a novel extraction process of a botanical fungicide allicin. The novel extraction process comprises the following steps: A, carrying out RO (Reverse Osmosis) treatment on tap water or mountain spring water to obtain enzymolysis water; b, performing enzyme activation treatment on the weakly alkaline water with mineralized low-potential small molecules; c, cleaning, sterilizing and squeezing the garlic through a cleaning machine, a sterilizer and a squeezer respectively; d, mixing the enzymolysis water, the weak base water and the garlic liquid, putting the mixture into a fermentation workshop for fermentation and catalytic reaction, and extracting the mixed liquid after 21 days; e, the extracting solution is filtered through a filter screen, liquid substances in the extracting solution are extracted, an activating agent needs to be used in enzyme activation treatment, and the activating agent is composed of phospholipid, buffer salt, citric acid, linden extracting solution, inorganic acid liquid, titanium tetraisopropylate, trehalose and aluminum potassium sulfate. Active substances of allicin are effectively reserved through low temperature, the process is simple, the active substances survive well, and the effect is better when the allicin is applied to plant sterilization.

Description

Technical Field

[0001] The invention relates to the technical field of allicin extraction, in particular to a novel extraction process of plant-derived fungicide allicin. Background Art

[0002] The bactericidal effect of allicin in agriculture is mainly reflected in the following aspects: 1. Broad-spectrum bactericidal: Allicin has a good killing effect on a variety of bacteria and fungi in the agricultural field, especially in the prevention and treatment of bacterial diseases. It can inhibit the synthesis of bacterial lipids and destroy the bacterial cell wall structure by combining with the amino acids necessary for bacterial growth and reproduction, thereby inhibiting the growth and reproduction of bacteria. At the same time, allicin can also inhibit a variety of amino acids in fungi, destroy the fungal spore structure, inhibit the synthesis of fungal proteins, and eventually make the spores lose vitality and inhibit the growth and reproduction of fungi; 2. Unique bactericidal mechanism: Allicin induces active autolysis and death of pathogens. Compared with other fungicides, its bactericidal effect is more thorough. In addition, allicin is extracted from pure plant sources. Compared with chemically synthesized fungicides, it is safer and has a wider range of applications; 3. Low drug resistance: Due to the unique bactericidal mechanism of allicin, it is not easy for bacteria to develop drug resistance to it, which enables allicin to maintain a high efficiency of bactericidal effect in long-term use; 4. Environmental protection and health: The residual amount of allicin in plants is far lower than the daily intake of garlic by the human body, and it is easily degraded in the natural environment. It is a green, environmentally friendly, safe and healthy "green pesticide"; 5. Promote plant growth: In addition to its bactericidal effect, allicin also has a good growth-promoting effect. Experimental studies have shown that allicin seed soaking and foliar spraying have different degrees of promotion on the germination and growth of a variety of vegetable seeds; 6. Wide range of prevention and control targets: Allicin has a good killing effect on a variety of bacterial diseases (such as canker, soft rot, bacterial angular spot, etc.) and fungal diseases (such as root rot, wilt, early blight, etc.) in the agricultural field. In summary, allicin is an efficient, safe and environmentally friendly fungicide in agriculture with broad application prospects.

[0003] The main bactericidal components of garlic are allicin and its various sulfur-containing compounds. Allicin is an organic sulfur compound extracted from the bulb (garlic head) of garlic, a plant of the genus Allium in the Allium family. It is also found in onions and other Allium plants. Its scientific name is diallyl thiosulfinate. In addition, garlic also contains more than 30 kinds of sulfur-containing compounds, such as DAS, DADS, DATS, and ajoene. These compounds also have certain bactericidal effects. The process of extracting organic sulfur compounds involves a variety of methods. These organic sulfur compounds mainly include allicin (diallyl The extraction methods of garlic organic sulfur compounds are: 1. Enzymatic steam distillation: In this method, garlic is crushed, and alliin is converted into allicin under the action of alliinase, and then extracted by steam distillation. However, the garlic oil extracted by this method has few impurities, but the content of allicin is very low and there is no active substance; 2. Organic solvent extraction: Use Organic solvents such as ethanol and ether are used to extract organic sulfides from garlic. This method can optimize the extraction effect by adjusting parameters such as solvent concentration, temperature, and extraction time. For example, studies have shown that the optimal extraction process parameters are 70% ethanol concentration, 2 hours enzyme reaction time, 50°C temperature, 1:7 (w / v) solid-liquid ratio, and 3 extractions. The extraction rate of garlic organic sulfides is 2.01 mg / g, but the secondary pollution is large; 3. Supercritical CO2 extraction method: This method uses CO2 in a supercritical state as a solvent to extract organic sulfides from garlic. Although the impurity content of garlic oil extracted by this method is high and the post-processing is troublesome, it can better retain the active ingredients in garlic, but the production cost is high; 4. Juice extraction method: The garlic juice containing organic sulfides is directly obtained by mechanically squeezing the crushed garlic paste, and then further separated and purified by organic solvent extraction or macroporous adsorption resin adsorption. The process flow is complicated; 5. Simultaneous distillation extraction method: This method combines the two technologies of distillation and extraction, which can simultaneously remove impurities and enrich target compounds during the extraction process. Through gas chromatography-mass spectrometry analysis, it can be learned that the main components of garlic organic sulfides obtained by this method are sulfide compounds, sulfur-containing ester compounds, etc., and there are no active substances; 6. Macroporous resin adsorption method: Use macroporous resin to adsorb and separate the organic sulfides in the crude garlic extract, and then obtain garlic oil with higher purity through elution and concentration steps. This method has the advantages of simple operation and low cost, but it has the characteristics of more waste. For this reason, we propose a new extraction process for the plant-based fungicide allicin. Summary of the invention

[0004] The purpose of the present invention is to provide a novel extraction process of plant-derived fungicide allicin to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solution: a novel extraction process of plant-derived fungicide allicin, comprising the following steps: A. Treat tap water or spring water with RO reverse osmosis to obtain enzymatic water; B. Enzyme activation treatment of weak alkaline water of mineralized low potential small molecules; C. The garlic is cleaned, sterilized and squeezed by a cleaning machine, a sterilizer and a squeezer respectively; D. Enzyme hydrolyzed water, weak alkaline water and garlic liquid are mixed and put into a fermentation workshop for fermentation and catalytic reaction, and the mixed solution is extracted after 21 days; E. Filter the extract through a filter to extract the liquid substance in the extract.

[0006] Preferably, the enzyme activation treatment requires the use of an activator, which is composed of phospholipids, buffer salts, citric acid, linden extract, inorganic acid solution, titanium tetraisopropoxide, trehalose and potassium aluminum sulfate, and its components in weight parts are: 7-12 parts of phospholipids; 3-8 parts of buffer salts; 4-9 parts of citric acid; 1-3 parts of linden extract; 2-6 parts of inorganic acid solution; 3-7 parts of titanium tetraisopropoxide; 2-4 parts of trehalose; and 10-15 parts of potassium aluminum sulfate.

[0007] Preferably, the cleaning machine is an ultrasonic cleaning machine, the sterilizer is an ultraviolet sterilizer, and the press is a twin-screw press.

[0008] Preferably, the fermentation temperature is 4-10 degrees Celsius, and a stirring mechanism is required during the fermentation process. The rotation speed of the stirring mechanism is 100-300r / min, and the stirring is performed for 5-10 minutes every 40-60 minutes.

[0009] Preferably, the mesh number of the filter is 200-300 meshes, the extracted liquid substance accounts for more than 95% of the total amount of the extract, and the substrate of the garlic fiber material is used as animal feed.

[0010] Compared with the prior art, the present invention has the following beneficial effects: The invention effectively retains the active substance of allicin through low temperature, has simple process, low production cost, no waste discharge, good survival of active substances, and better effect in plant sterilization. DETAILED DESCRIPTION

[0011] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0012] A novel extraction process of plant-derived fungicide allicin comprises the following steps: A. Treat tap water or spring water with RO reverse osmosis to obtain enzymatic water; B. Enzyme activation treatment of weak alkaline water of mineralized low potential small molecules; C. The garlic is cleaned, sterilized and squeezed by a cleaning machine, a sterilizer and a squeezer respectively; D. Enzyme hydrolyzed water, weak alkaline water and garlic liquid are mixed and put into a fermentation workshop for fermentation and catalytic reaction, and the mixed solution is extracted after 21 days; E. Filter the extract through a filter to extract the liquid substance in the extract.

[0013] The active substance of allicin is effectively retained through low temperature, and the process is simple, the production cost is low, there is no waste discharge, and the active substance has good survival, so it is more effective in plant sterilization.

[0014] The enzyme activation treatment requires the use of an activator, which is composed of phospholipids, buffer salts, citric acid, linden extract, inorganic acid, titanium tetraisopropoxide, trehalose and potassium aluminum sulfate. The components in weight parts are: 7-12 parts of phospholipids; 3-8 parts of buffer salts; 4-9 parts of citric acid; 1-3 parts of linden extract; 2-6 parts of inorganic acid; 3-7 parts of titanium tetraisopropoxide; 2-4 parts of trehalose; and 10-15 parts of potassium aluminum sulfate.

[0015] The cleaning machine is an ultrasonic cleaning machine, the sterilizer is an ultraviolet sterilizer, and the press is a twin-screw press.

[0016] The fermentation temperature is 4-10 degrees Celsius. During the fermentation process, it needs to be used in conjunction with a stirring mechanism. The speed of the stirring mechanism is 100-300r / min, and it is stirred for 5-10 minutes every 40-60 minutes.

[0017] The mesh number of the filter is 200-300 meshes, and the extracted liquid substance accounts for more than 95% of the total amount of the extract, and the substrate of the garlic fiber material is used as animal feed.

[0018] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A novel extraction process of plant-derived fungicide allicin, characterized by: The following steps are involved: A. Treat tap water or spring water with RO reverse osmosis to obtain enzymatic water; B. Enzyme activation treatment of weak alkaline water of mineralized low potential small molecules; C. The garlic is cleaned, sterilized and squeezed by a cleaning machine, a sterilizer and a squeezer respectively; D. Enzyme hydrolyzed water, weak alkaline water and garlic liquid are mixed and put into a fermentation workshop for fermentation and catalytic reaction, and the mixed solution is extracted after 21 days; E. Filter the extract through a filter to extract the liquid substance in the extract.

2. The novel extraction process of the botanical fungicide garlicin according to claim 1, characterized in that: The enzyme activation treatment requires the use of an activator, which is composed of phospholipids, buffer salts, citric acid, linden extract, inorganic acid solution, titanium tetraisopropoxide, trehalose and potassium aluminum sulfate, and its components in weight parts are: 7-12 parts of phospholipids; 3-8 parts of buffer salts; 4-9 parts of citric acid; 1-3 parts of linden extract; 2-6 parts of inorganic acid solution; 3-7 parts of titanium tetraisopropoxide; 2-4 parts of trehalose; and 10-15 parts of potassium aluminum sulfate.

3. The novel extraction process of the botanical fungicide garlicin according to claim 1 is characterized in that: The cleaning machine is an ultrasonic cleaning machine, the sterilizer is an ultraviolet sterilizer, and the press is a twin-screw press.

4. The novel extraction process of the botanical fungicide garlicin according to claim 1 is characterized in that: The fermentation temperature is 4-10 degrees Celsius. During the fermentation process, a stirring mechanism is required. The rotation speed of the stirring mechanism is 100-300r / min, and the stirring is performed for 5-10 minutes every 40-60 minutes.

5. The novel extraction process of the botanical fungicide garlicin according to claim 1 is characterized in that: The mesh number of the filter is 200-300 meshes, and the extracted liquid substance accounts for more than 95% of the total amount of the extract, and the substrate of the garlic fiber material is used as animal feed.