Microbial flora mother liquor culture device and culture method

Abstract

The invention relates to the field of microbial culture, and discloses a microbial flora mother liquor culture device and a culture method.The microbial flora mother liquor culture device comprises a water tank, a culture bin and an oxygen supply device, the water tank is communicated with the culture bin through a water pipe, a plurality of biological stuffing boxes are installed in the culture bin, and the multiple biological stuffing boxes are annularly arranged in an inner layer and an outer layer; a cavity is formed in the center of the culture bin. By arranging the inner-layer biological stuffing box and the outer-layer biological stuffing box, the attachment area and permeability of microorganisms can be improved, so that the breeding number of the microorganisms is increased, and the space between the outer shell and the inner shell is filled with the biological stuffing through the biological stuffing boxes of the frame structure, so that the demolding efficiency of microbial flora can be effectively improved; and in addition, the demolding assembly can enable the biological stuffing box to rotate and shake in a reciprocating manner, so that the demolding efficiency of the microbial flora is further improved, and the sewage purification efficiency is improved.

Description

Technical Field

[0001] The invention relates to the field of microbial culture, and in particular to a microbial flora mother liquid culture device and a culture method. Background Art

[0002] With the development of the times, the use of microbial flora for water treatment has been widely used. The existing method is to use oxygen supply devices and water supply devices to supply air and nutrition to allow microorganisms to grow on biological fillers and reproduce, and then remove the membranes, and continuously output microbial flora mother liquor containing various microorganisms to the surrounding polluted water bodies. The microbial flora mother liquor nitrifies and denitrifies various suspended solids, organic matter, and various harmful pollutants in the polluted water body, thereby achieving microbial purification of sewage.

[0003] There are still many problems with the above treatment methods, such as: 1. During the use of existing biological fillers, microorganisms multiply and demold, and the demolding rate is low, which makes the density of microbial mother liquid in water low, reducing the sewage purification effect. 2. At present, the microbial mother liquid is usually discharged through the overflow pipe and enters the sewage. The amount of mother liquid that gradually overflows is small and the penetration in the sewage is slow. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a microbial flora mother liquid cultivation device, which changes the filler method and filler structure as well as the discharge method of the microbial flora mother liquid, improves the film removal rate and the content of the microbial flora mother liquid in sewage, thereby improving the sewage purification efficiency.

[0005] The present invention provides a microbial flora mother liquid culture device, comprising a water tank, a culture bin, and an oxygen supply device, wherein the water tank is connected to the culture bin through a water pipe, a biological stuffing box is installed in the culture bin, a plurality of the biological stuffing boxes are arranged in a ring shape and are arranged in two layers, the center of the culture bin is a cavity, the upper and lower ends of the biological stuffing box are respectively clamped on two symmetrically arranged fixed components, the biological stuffing box comprises an outer shell and an inner shell, biological stuffing is filled between the outer shell and the inner shell, the outer shell is a frame structure, a rolling frame for demoulding is installed around the frame, and conical through holes are provided around the inner shell;

[0006] The fixing assembly includes three annular tubes and brackets, the three annular tubes are fixedly connected by a plurality of brackets, and the oxygen supply device is fixed and connected to the annular tubes; the culture bin is also provided with an automatic water supply and drainage mechanism, and a collecting bin is provided on one side of the culture bin, and the collecting bin collects sewage in the culture bin through the automatic water supply and drainage mechanism;

[0007] The annular tube is connected to a demoulding assembly, and the demoulding assembly rotates and reciprocates the biological filling box to demould the microorganisms.

[0008] The present invention provides a microbial flora mother liquid culture device, wherein the demoulding component includes a lifting component and a vibration component, the vibration component includes a motor, the mounting plate of the motor is fixed to the lifting component, the output end of the motor is coaxially fixed to the first rotating shaft, the first rotating shaft is fixed to the first disc, the first guide slider is fixed on the first disc, the first guide slider is slidably connected to the first guide rail, the first guide rail is vertically fixed to the second guide rail, the second guide rail is slidably connected to the second guide slider, the second guide slider is slidably connected to the second disc, the second disc is coaxially fixed to the second rotating shaft, an eccentric block is fixed on the second rotating shaft, the second rotating shaft is connected to the third slider bearing, the third slider moves along the rectangular frame, the rectangular frame is fixedly connected to the first support rod, the first support rod is fixed to the mounting plate of the motor, the two sides of the third slider are respectively fixed to one end of the first spring and the second spring, the first spring and the second spring are fixed to the rectangular frame, the second rotating shaft is fixed to the second support rod, and the second support rod is fixed to the annular tube.

[0009] The present invention provides a microbial flora mother liquid culture device, wherein the lifting component comprises a cylinder, the cylinder is fixed to a support frame, and the output end of the cylinder is fixed to a mounting plate of the motor.

[0010] The present invention provides a microbial flora mother liquid culture device, wherein the two ends of the outer shell and the inner shell are respectively clamped with a blocking plate, the blocking plate is evenly provided with water leakage holes, the outer shell is provided with mounting holes around, each mounting hole is provided with the rolling frame, the rolling frame includes a wave plate and a rotating wheel, a plurality of the wave plates and the rotating wheels are alternately arranged on the mounting hole, and the wave plates and the rotating wheels are rotatably connected to the inner wall of the mounting hole.

[0011] The present invention provides a microbial flora mother liquid culture device, wherein the rotating wheel comprises a rotating shaft and blades, and a plurality of blades are fixed on the rotating shaft.

[0012] The present invention provides a microbial flora mother liquid culture device, wherein the automatic water supply and drainage mechanism includes a lifting plate, the lifting plate is provided with an annular groove, the annular tube is attached to the annular groove, the lifting plate is fixed to a third support rod, the third support rod is fixed to the rectangular frame, a water inlet hole is provided at the center of the lifting plate, and the water inlet hole is rotatably connected to the baffle.

[0013] The present invention provides a microbial flora mother liquid culture device, wherein the oxygen supply device comprises an oxygen supply pipe, the oxygen supply pipe is C-shaped, the upper and lower sides of the oxygen supply pipe are respectively fixed to and connected with the top and bottom annular pipes, and the oxygen supply pipe is connected to an oxygen supply machine.

[0014] The present invention provides a method for culturing a microbial flora mother solution, comprising the following steps:

[0015] S1, firstly, the sewage flows from the water tank into the culture chamber;

[0016] S2, start the lifting component, put the biological filler box into the culture chamber, the sewage fills the area around the biological filler box, and the microorganisms in the sewage begin to form biofilms and reproduce;

[0017] S3, detecting the concentration of microorganisms in the culture chamber, preparing to discharge the mold and discharge the mother liquid of the microbial flora;

[0018] S4, start the demoulding component to rotate and reciprocate the biological stuffing box to achieve demoulding, and lift the biological stuffing box out of the culture chamber. During the lifting process, the mother liquid of the microbial flora overflows into the collection chamber, and the culture chamber is refilled with sewage through the automatic water supply and drainage mechanism;

[0019] S5, the water tank is replenished with sewage.

[0020] The microbial flora mother liquid culture device and culture method of the present invention are different from the prior art in that:

[0021] 1. The present invention can increase the attachment area and permeability of microorganisms by setting up two layers of inner and outer biological filler boxes, thereby increasing the number of microorganisms to reproduce. The biological filler box with a frame structure fills the outer shell and the inner shell with biological fillers, which can effectively improve the demolding efficiency of the microbial flora, thereby increasing the density of the microbial flora in the sewage. Furthermore, the demolding component can make the biological filler box rotate and reciprocate, thereby further improving the demolding efficiency of the microbial flora and improving the sewage purification efficiency.

[0022] 2. The present invention provides attachment and growth space for microorganisms by means of a rolling frame and a wave plate arranged on the outer shell. In addition, during the lifting process, the rotating wheel can rotate by itself by relying on the thrust of the water flow, thereby generating turbulence in the sewage, improving the demoulding efficiency, and allowing the biological flora to spread rapidly.

[0023] The following is a further description of a microbial flora mother liquid culture device and a culture method of the present invention in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The present invention is a front view of a microbial flora mother liquid culture device and a culture method;

[0025] Figure 2 yes Figure 1 An isometric view of a microbial flora mother liquid culture device and a culture method;

[0026] Figure 3 yes Figure 1 A partial exploded view of

[0027] Figure 4 It is an axonometric view of the biological stuffing box;

[0028] Figure 5 yes Figure 4 Exploded view of;

[0029] Figure 6 It is a schematic diagram of the structure of the rolling frame;

[0030] Figure 7 yes Figure 6 A partial enlarged view of . DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] See also Figure 1 - Figure 7 The present invention provides a technical solution, which specifically includes the following embodiments:

[0033] Embodiment 1:

[0034] A microbial flora mother liquid culture device comprises a water tank 1, a culture bin 2, and an oxygen supply device 3. The water tank 1 is connected to the culture bin 2 through a water pipe 11. Sewage is stored in the water tank 1 and can be supplemented with sewage from the outside at any time through a water pump. A one-way valve is installed on the water pipe 11 to prevent the sewage in the culture bin 2 from flowing back. A biological stuffing box 4 is installed in the culture bin 2. A plurality of biological stuffing boxes 4 are arranged in an annular shape and have an inner and outer layer. The center of the culture bin 2 is a cavity, so that it is easier to demold the microbial flora. The upper surface of the biological stuffing box 4 The lower two ends are respectively clamped on two symmetrically arranged fixing components 5. The biological filler box 4 includes an outer shell 41 and an inner shell 42. The inner shell 42 is inserted into the outer shell 41. The biological filler is filled between the outer shell 41 and the inner shell 42. The outer shell 41 is a frame structure. A rolling frame 401 for demoulding is installed around the frame. Conical through holes 402 are opened around the inner shell 42. The conical through holes 402 are larger in the direction close to the outer shell and smaller in the direction away from the outer shell. This can prevent large particles in sewage from entering the filler and extend the service life of the biological filler.

[0035] The fixing assembly 5 includes three annular tubes 51 and a bracket 52. The three annular tubes 51 are fixedly connected by a plurality of brackets 52. The bracket 52 is also arranged in an annular shape. The oxygen supply device 3 is fixed and communicated with the annular tubes 51. The annular tubes 51 are hollow inside and have air holes for releasing oxygen. The culture bin 2 is also provided with an automatic water supply and drainage mechanism 7. A collecting bin 6 is provided on one side of the culture bin 2. The collecting bin 6 collects sewage in the culture bin 2 through the automatic water supply and drainage mechanism 7.

[0036] The annular tube 51 is connected to the demoulding assembly 8, and the demoulding assembly 8 causes the biological filling box 4 to rotate and reciprocate to demould the microorganisms.

[0037] During operation, sewage flows from the water tank 1 into the culture bin 2, and the microorganisms in the sewage attach to the biological filler box 4. After the oxygen supply device 3 supplies oxygen through the annular tube 51 and reproduces for a certain period of time, the demoulding component 8 is started to rotate the biological filler box 4 and shake it back and forth to demould the microbial flora. The formed microbial flora mother liquid can quickly and evenly penetrate into the sewage, thereby reacting and degrading harmful pollutants in the sewage and purifying the sewage. The sewage containing the microbial flora mother liquid in the culture bin 2 flows into the collection bin 6 through the automatic water supply and drainage mechanism 7. At the same time, new sewage is poured into the culture bin 2, and the microbial flora mother liquid is re-cultured to ensure that the content of the microbial flora mother liquid in the sewage is stable.

[0038] The present invention can increase the attachment area and permeability of microorganisms by setting up inner and outer layers of biological stuffing boxes 4, thereby increasing the number of microorganisms to reproduce. The biological stuffing box 4 with a frame structure fills the outer shell 41 and the inner shell 42 with biological stuffing, which can effectively improve the demolding efficiency of the microbial flora, thereby increasing the density of the microbial flora in the sewage. Furthermore, the demolding component 8 can make the biological stuffing box 4 rotate and reciprocate, thereby further improving the demolding efficiency of the microbial flora and improving the sewage purification efficiency.

[0039] As a further explanation of this embodiment, the demoulding component 8 includes a lifting component 9 and a vibration component 80, the vibration component 80 includes a motor 801, the mounting plate of the motor 801 is fixed to the lifting component 9, the output end of the motor 801 is coaxially fixed to the first rotating shaft 802, the first rotating shaft 802 is fixed to the first disc 803, the first disc 803 is fixed with a first guide slider 804, the first guide slider 804 is slidably connected to the first guide rail 805, the first guide rail 805 is vertically fixed to the second guide rail 806, the second guide rail 806 is slidably connected to the second guide slider 807, and the second guide slider 807 is slidably connected to the second disc 816. The second disc 816 is coaxially fixed with the second rotating shaft 808, the eccentric block 809 is fixed on the second rotating shaft 808, the second rotating shaft 808 is bearing-connected with the third slider 810, the third slider 810 moves along the rectangular frame 811, the rectangular frame 811 is fixedly connected with the first support rod 812, the first support rod 812 is fixed to the mounting plate of the motor 801, the two sides of the third slider 810 are respectively fixed to one end of the first spring 813 and the second spring 814, the first spring 813 and the second spring 814 are fixed to the rectangular frame 811, the second rotating shaft 808 is fixed to the second support rod 815, and the second support rod 815 is fixed to the annular tube 51.

[0040] The lifting assembly 9 includes a cylinder 91 , which is fixed to a support frame 92 , and an output end of the cylinder 91 is fixed to a mounting plate of the motor 801 .

[0041] During operation, when the microorganism flora is demoulded, the motor 801 is started to drive the first rotating shaft 802 to rotate, so that the first disc 803 drives the second disc 816 to rotate, and then the second rotating shaft 808 is rotated, so that the eccentric block 809 is rotated, and the third slider 810 reciprocates along the rectangular frame 811, so that the second rotating shaft 808 reciprocates while rotating, so that the biological filling box 4 reciprocates while rotating, so that the microorganism flora is demoulded. And after the demoulding component 8 has worked for a period of time, the lifting component 9 is started, so that the cylinder 91 drives the support frame 92 to lift, drives the demoulding component to rise and lifts the biological filling box 4 out of the culture chamber 2. During the lifting process, the microorganism flora can fall off more thoroughly, and the penetration of the microorganism flora in the sewage is accelerated during the lifting process, thereby improving the sewage purification efficiency.

[0042] The culture chamber 2 and the demoulding assembly 8 are externally covered with dustproof shells.

[0043] As a further explanation of the present embodiment, the two ends of the outer shell 41 and the inner shell 42 are respectively clamped with the blocking plate 403, the blocking plate 403 is evenly provided with leakage holes 404, the outer shell 41 is provided with mounting holes 405 around, each mounting hole 405 is provided with the rolling frame 401, the rolling frame 401 includes a wave plate 4001 and a rotating wheel 4002, a plurality of the wave plates 4001 and the rotating wheels 4002 are alternately arranged on the mounting hole 405, the wave plates 4001 and the rotating wheels 4002 are rotatably connected to the inner wall of the mounting hole 405, the rotating wheel 4002 includes a rotating shaft 4003 and fan blades 4004, and a plurality of the fan blades 4004 are fixed on the rotating shaft 4003.

[0044] The present invention provides microorganisms with attachment and growth space by means of a rolling frame 401 and a wave plate 4001 arranged on a shell 41, and during the lifting process, the rotating wheel 4002 can rotate by the thrust of the water flow, thereby generating turbulence in the sewage, improving the demoulding efficiency, and allowing the biological flora to spread rapidly.

[0045] As a further explanation of this example, the automatic water supply and drainage mechanism 7 includes a lifting plate 71, the lifting plate 71 has an annular groove 72, the annular tube 51 is attached to the annular groove 72, the lifting plate 71 is fixed to a third support rod 73, the third support rod 73 is fixed to the rectangular frame 811, and a water inlet hole 75 is provided in the center of the lifting plate 71, and the water inlet hole 75 is rotatably connected to the baffle 74.

[0046] During operation, the lifting plate 71 can move upwards by being lifted by the third support rod 73. In the process of lifting the biological stuffing box 4 out of the culture chamber 2, the lifting plate 71 lifts the sewage to the collecting chamber 6. The lifting plate 71 divides the culture chamber 2 into two parts. In the lifting process, the microbial flora mother liquid is above the lifting plate 71, and the sewage is below the lifting plate 71. The sewage gradually flows in from the water tank 1, and the microbial flora mother liquid gradually enters the collecting chamber 6. When the biological stuffing box 4 is lifted out of the culture chamber 2, the sewage occupies the original position of the microbial flora mother liquid, and the microbial flora mother liquid all enters the collecting chamber 6. When the biological stuffing box 4 falls, the baffle 74 is opened by the pressure of the sewage and fills the area around the biological stuffing box 4.

[0047] The space of the annular groove 72 can allow the lifting plate 71 to rotate and shake left and right.

[0048] As a further explanation of this example, the oxygen supply device 3 includes an oxygen supply pipe 30, which is C-shaped. The upper and lower sides of the oxygen supply pipe 30 are respectively fixed and connected to the top and bottom annular pipes 51, and the oxygen supply pipe 30 is connected to the oxygen supply machine.

[0049] The present invention oxygenates the upper and lower annular tubes 51 through the oxygen supply tube 30, so that the oxygen is evenly diffused in the sewage, which can improve the survival rate of microorganisms and further improve the reproduction efficiency.

[0050] A method for culturing a microbial flora mother solution comprises the following steps:

[0051] S1, firstly, the sewage flows from the water tank 1 into the culture chamber 2;

[0052] S2, start the lifting component 9, put the biological filler box 4 into the culture chamber 2, the sewage fills the area around the biological filler box 4, and the microorganisms in the sewage begin to form biofilms and reproduce;

[0053] S3, detecting the concentration of microorganisms in the culture chamber 2, preparing to discharge the mold and discharge the mother liquid of the microorganism flora;

[0054] S4, start the demoulding assembly 8, make the biological stuffing box 4 rotate and reciprocate to achieve demoulding, and lift the biological stuffing box 4 out of the culture chamber 2. During the lifting process, the mother liquid of the microbial flora overflows into the collection chamber 6, and the culture chamber 2 is refilled with sewage through the automatic water supply and drainage mechanism 7;

[0055] S5, water tank 1 is replenished with sewage.

Claims

1. A microbial flora mother liquid culture device, comprising a water tank (1), a culture chamber (2), and an oxygen supply device (3), characterized in that: The water tank (1) is connected to the culture chamber (2) through a water pipe (11); a biological stuffing box (4) is installed in the culture chamber (2); a plurality of biological stuffing boxes (4) are arranged in a ring shape and are arranged in two layers, the center of the culture chamber (2) is a cavity; the upper and lower ends of the biological stuffing box (4) are respectively clamped on two symmetrically arranged fixing components (5); the biological stuffing box (4) comprises an outer shell (41) and an inner shell (42); biological stuffing is filled between the outer shell (41) and the inner shell (42); the outer shell (41) is a frame structure; a rolling frame (401) for demoulding is installed around the frame; and conical through holes (402) are provided around the inner shell (42); The fixing assembly (5) comprises three annular tubes (51) and brackets (52); the three annular tubes (51) are fixedly connected to each other via a plurality of brackets (52); the oxygen supply device (3) is fixedly connected to and communicates with the annular tubes (51); the culture chamber (2) is also provided with an automatic water supply and drainage mechanism (7); a collecting chamber (6) is provided on one side of the culture chamber (2); the collecting chamber (6) collects sewage in the culture chamber (2) via the automatic water supply and drainage mechanism (7); The annular tube (51) is connected to a demoulding assembly (8), and the demoulding assembly (8) causes the biological filling box (4) to rotate and reciprocate to demould the microorganisms.

2. A microbial flora mother liquid culture device according to claim 1, characterized in that: The demoulding assembly (8) comprises a lifting assembly (9) and a vibration assembly (80), wherein the vibration assembly (80) comprises a motor (801), wherein a mounting plate of the motor (801) is fixed to the lifting assembly (9), wherein an output end of the motor (801) is coaxially fixed to a first rotating shaft (802), wherein the first rotating shaft (802) is fixed to a first disc (803), wherein a first guide slider (804) is fixed to the first disc (803), wherein the first guide slider (804) is slidably connected to a first guide rail (805), wherein the first guide rail (805) is vertically fixed to a second guide rail (806), wherein the second guide rail (806) is slidably connected to a second guide slider (807), wherein the second guide slider (807) is slidably connected to a second disc (816), and wherein the second The disc (816) is coaxially fixed with the second rotating shaft (808), an eccentric block (809) is fixed on the second rotating shaft (808), the second rotating shaft (808) is bearing-connected with the third slider (810), the third slider (810) moves along the rectangular frame (811), the rectangular frame (811) is fixedly connected with the first support rod (812), the first support rod (812) is fixed with the mounting plate of the motor (801), the two sides of the third slider (810) are respectively fixed with one end of the first spring (813) and the second spring (814), the first spring (813) and the second spring (814) are fixed with the rectangular frame (811), the second rotating shaft (808) is fixed with the second support rod (815), and the second support rod (815) is fixed with the annular tube (51).

3. A microbial flora mother liquid culture device according to claim 2, characterized in that: The lifting assembly (9) comprises a cylinder (91), the cylinder (91) is fixed to a support frame (92), and the output end of the cylinder (91) is fixed to a mounting plate of the motor (801).

4. A microbial flora mother liquid culture device according to claim 1, characterized in that: The two ends of the outer shell (41) and the inner shell (42) are respectively clamped with a blocking plate (403); the blocking plate (403) is evenly provided with water leakage holes (404); the outer shell (41) is provided with mounting holes (405) around it; each mounting hole (405) is provided with the rolling frame (401); the rolling frame (401) comprises a wave plate (4001) and a rotating wheel (4002); a plurality of the wave plates (4001) and the rotating wheels (4002) are alternately arranged on the mounting hole (405); the wave plates (4001) and the rotating wheels (4002) are rotatably connected to the inner wall of the mounting hole (405).

5. A microbial flora mother liquid culture device according to claim 4, characterized in that: The rotating wheel (4002) comprises a rotating shaft (4003) and blades (4004), and a plurality of blades (4004) are fixed on the rotating shaft (4003).

6. A microbial flora mother liquid culture device according to claim 1, characterized in that: The automatic water supply and drainage mechanism (7) comprises a lifting plate (71), the lifting plate (71) is provided with an annular groove (72), the annular tube (51) is attached to the annular groove (72), the lifting plate (71) is fixed to a third support rod (73), the third support rod (73) is fixed to the rectangular frame (811), a water inlet hole (75) is provided at the center of the lifting plate (71), and the water inlet hole (75) is rotatably connected to a baffle (74).

7. A microbial flora mother liquid culture device according to claim 6, characterized in that: The oxygen supply device (3) comprises an oxygen supply pipe (30), the oxygen supply pipe (30) is C-shaped, the upper and lower sides of the oxygen supply pipe (30) are respectively fixed to and connected with the annular pipes (51) at the top and bottom, and the oxygen supply pipe (30) is connected with an oxygen supply machine.

8. A method for culturing a microbial flora mother solution, based on a microbial flora mother solution culturing device according to any one of claims 1 to 7, characterized in that: The steps include: S1, firstly, the sewage flows from the water tank (1) into the culture chamber (2); S2, start the lifting component (9), put the biological filler box (4) into the culture chamber (2), the sewage fills the area around the biological filler box (4), and the microorganisms in the sewage begin to grow biofilms; S3, detecting the concentration of microorganisms in the culture chamber (2), preparing to discharge the mold and discharge the mother liquid of the microorganism flora; S4, starting the demoulding assembly (8), causing the biological stuffing box (4) to rotate and reciprocate to achieve demoulding, and lifting the biological stuffing box (4) out of the culture chamber (2). During the lifting process, the mother liquid of the microbial flora overflows into the collection chamber (6), and the culture chamber (2) is refilled with sewage through the automatic water supply and drainage mechanism (7); S5, water tank (1) is replenished with sewage.

Images