A device and method for evaluating the sterilization efficiency of ultraviolet LED lamp beads

Abstract

The present invention relates to the technical field of microbial detection devices, and in particular to an ultraviolet LED lamp bead sterilization efficiency evaluation device and method. The device comprises: a lamp bead support rod is vertically installed on the side of a slide limiting platform, a lamp bead limiting clamp is installed on the lamp bead support rod and is level with the slide limiting platform; a concave disc-shaped slide is placed on the slide limiting platform; the platform support legs are installed below the slide limiting platform; a scale height adjustment device is provided at the upper end of the lamp bead support rod for adjusting the height of the lamp bead support rod; a fixed positioning rod is provided at the 0 scale position of the lamp bead support rod; an irradiance test hole is provided on the slide limiting platform; the lamp bead limiting clamp is provided with a clamp limiting hole, a lamp bead limiting groove and a clamp fixing port, and is sleeved on the lamp bead support rod through the clamp limiting hole; the lamp bead limiting groove is used to place the test lamp bead. The advantages are: reducing errors caused by operation or random factors; and increasing the reliability of the evaluation of the efficiency of ultraviolet lamp bead in inactivating microorganisms.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial detection devices, and in particular to a device and method for evaluating the sterilization efficiency of ultraviolet LED lamp beads. Background Art

[0002] Due to its short wavelength and high photon energy, UV light can effectively destroy the DNA / RNA genetic material or protective structures in viruses and bacteria, thereby inactivating microorganisms. It is considered a broad-spectrum, highly efficient light source for inactivating viruses and bacteria. Mercury lamp-based UV light sources are gradually being phased out. Semiconductor-based UV LEDs, with their advantages of small size, concentrated emission wavelength, no ozone generation, and environmental friendliness, are the primary light source to replace mercury lamps in the field of microbial inactivation and will play a key role in medical and other fields.

[0003] As semiconductor ultraviolet LED lamps are gradually applied in the field of microbial inactivation, accurate testing of the inactivation efficiency of LED lamps from different manufacturers and batches has become a key issue. The main factors affecting the inactivation efficiency of deep ultraviolet LED lamps are as follows: 1. The wavelength of the lamp bead: Due to the differences in the protective structure, genetic material type, and size of different microorganisms, they have different inactivation sensitivities to different wavelengths; 2. The power density of the ultraviolet light (i.e., irradiance) received by the microorganism: Under the same wavelength irradiation conditions, the higher the irradiance received by the microorganism, the better the inactivation effect; 3. Other environmental factors: Such as the distance between the microorganism and the lamp bead, the ambient humidity, etc., mainly affect the inactivation efficiency by affecting the irradiance received by the microorganism.

[0004] However, existing UV lamp inactivation methods suffer from inconsistent and inaccurate inactivation detection processes. These processes are affected by a variety of random factors, including operator technique, the dosage of the microbial droplets used, and the shape of the droplets. This reduces the reliability of evaluating the inactivation efficacy of LED products and hinders accurate testing and comparison of the inactivation efficiency of different lamps. Summary of the Invention

[0005] In order to solve the above problems, the present invention provides a device and method for evaluating the sterilization efficiency of ultraviolet LED lamp beads.

[0006] The first object of the present invention is to provide a UV LED lamp bead sterilization efficiency evaluation device, comprising: a slide limiting platform, a lamp bead support rod, a concave disc-shaped slide, a lamp bead limiting fixture and a platform support leg;

[0007] The lamp bead support rod is vertically installed on the side of the slide limiting platform, and the lamp bead limiting fixture is installed on the lamp bead support rod and is horizontal to the slide limiting platform; the concave disc type slide is placed on the slide limiting platform; the platform support legs are installed under the slide limiting platform to support the slide limiting platform;

[0008] The upper end of the lamp bead support rod is provided with a scale height adjustment device for adjusting the height of the lamp bead support rod; a fixed positioning rod is set at the 0 scale position of the lamp bead support rod;

[0009] The slide limiting platform is provided with an irradiance test hole, which is used to align the luminous center of the lamp bead with the center of the concave disc slide; the size of the irradiance test hole is consistent with the UV radiometer used;

[0010] The lamp bead limiting fixture is provided with a fixture limiting hole, a lamp bead limiting groove and a fixture fixing mouth, and is connected to the lamp bead support rod through the fixture limiting hole; after the lamp bead limiting fixture is adjusted to the predetermined position along the lamp bead support rod, the lamp bead limiting fixture is fixed to the lamp bead support rod by locking the fixture fixing mouth; the lamp bead limiting groove is used to place the test lamp bead to ensure that the position of the lamp bead is consistent for each test.

[0011] Preferably, the concave dish-type slide is provided with a concave dish and a liquid collecting groove, the concave dish is used to hold the microbial liquid, and the liquid collecting groove is used to collect excess liquid.

[0012] Preferably, the area of ​​the concave disk is the same as the effective detection area of ​​the ultraviolet irradiator used, and the diameter of the concave disk does not exceed 1 cm and the depth does not exceed 1 mm.

[0013] Preferably, a slide positioning rod is provided on one side of the concave disc slide, and the slide positioning rod extends to the fixed positioning rod of the lamp bead support rod; by comparing whether the slide positioning rod and the upper surface of the fixed positioning rod are horizontal, it is determined whether the upper surface of the microbial liquid is aligned with the 0 scale of the lamp bead support rod.

[0014] Preferably, the fixed positioning rod is connected to the detachable positioning rod; the detachable positioning rod is used to compare whether the 0 scale line of the lamp bead support rod and the upper surface of the ultraviolet irradiator are on the same plane.

[0015] Preferably, the sterilization is the inactivation of viruses or bacteria.

[0016] The second object of the present invention is to provide a method for evaluating the sterilization efficiency of ultraviolet LED lamp beads, which uses the above-mentioned device for evaluation, including the following steps:

[0017] S1. Fix the UV irradiance meter's photosensitive surface to the irradiance test port on the slide limiter platform. Use the fixed positioning rod of the lamp support rod to align the UV irradiance meter's photosensitive surface with the 0 scale position of the lamp support rod.

[0018] S2. Adjust the lamp limit fixture to the specified scale, turn on the lamp using the required test current, measure the lamp irradiance under the experimental conditions, and record it.

[0019] S3. Place the concave disc slide on the slide limit platform and use the slide positioning rod to align the concave disc slide upper surface with the 0 scale position of the lamp support rod;

[0020] S4. Align the center of the concave plate with the center of the lamp bead; drop the microbial liquid into the concave plate and use the flat plate to hang the excess liquid;

[0021] S5. Turn on the lamp beads to perform inactivation experiments;

[0022] S6. After the experiment is over, turn off the lamp beads, draw a certain amount of inactivated microbial liquid, and then perform culture or biological tissue infection experiments to confirm the microbial inactivation rate of the lamp beads; safely dispose of materials that have come into contact with the microbial liquid.

[0023] Preferably, in step S4, the volume of the microbial liquid dripped into the concave dish is at least 5% larger than the volume of the concave dish, ensuring that the microbial liquid fills the concave dish; and the excess liquid is scraped into the liquid collection tank of the concave dish slide.

[0024] Preferably, in step S3, the detachable positioning rod on the lamp bead support rod is first removed, the concave disc type slide is placed on the slide limiting platform, and the slide positioning rod is used to make the upper surface of the concave disc type slide flush with the upper surface of the fixed positioning rod.

[0025] Preferably, the flat plate is a disposable flat plate made of hydrophobic material.

[0026] Compared with the prior art, the present invention can achieve the following beneficial effects:

[0027] Through the strict limit operation of the device, errors caused by random factors such as the test operator's technique, solution dosage, microbial droplet dosage, droplet shape, etc. can be prevented, thereby increasing the reliability of the evaluation of the efficiency of ultraviolet lamp beads in inactivating microorganisms. Different batches of lamp beads can be evaluated with very small errors. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a schematic diagram of the overall structure of the ultraviolet LED lamp bead sterilization efficiency evaluation device provided according to an embodiment of the present invention.

[0029] Figure 2 2 is a schematic structural diagram of a slide limiting platform provided according to an embodiment of the present invention.

[0030] Figure 3 2 is a schematic diagram of the structure of a concave disc type glass slide provided according to an embodiment of the present invention.

[0031] Figure 4 2 is a schematic diagram of the structure of a lamp bead limiting clamp provided according to an embodiment of the present invention.

[0032] Reference numerals:

[0033] 1. Slide limiting platform;

[0034] 101. Irradiance test hole;

[0035] 2. Lamp support rod;

[0036] 201. Scale height adjustment device; 202. Fixed positioning rod; 203. Removable positioning rod;

[0037] 3. Concave disc slide;

[0038] 301. Slide positioning rod; 302. Concave plate; 303. Liquid collection tank;

[0039] 4. Lamp bead limiting fixture;

[0040] 401. Clamp fixing port; 402. Clamp limiting hole; 403. Lamp bead limiting slot;

[0041] 5. Platform support legs. DETAILED DESCRIPTION

[0042] Hereinafter, embodiments of the present invention will be described with reference to the accompanying drawings. In the following description, identical modules are denoted by identical reference numerals. In the case of identical reference numerals, their names and functions are also identical. Therefore, their detailed description will not be repeated.

[0043] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and do not constitute a limitation of the present invention.

[0044] See also Figures 1-4 The present invention provides an ultraviolet LED lamp bead sterilization efficiency evaluation device, comprising: a slide limiting platform 1, a lamp bead support rod 2, a concave disc type slide 3, a lamp bead limiting fixture 4 and a platform support leg 5;

[0045] The lamp bead support rod 2 is vertically mounted on the side of the slide limiting platform 1, and the lamp bead limiting fixture 4 is mounted on the lamp bead support rod 2 and is horizontal to the slide limiting platform 1; the lamp bead limiting fixture 4 can move up and down along the direction of the lamp bead support rod 2 and can be completely fixed in a selected position;

[0046] The concave disc type slide glass 3 is placed on the slide limiting platform 1;

[0047] The platform support legs 5 are installed below the slide limiting platform 1 and are used to support the slide limiting platform 1;

[0048] The upper end of the lamp bead support rod 2 is provided with a scale height adjustment device 201 for adjusting the height of the lamp bead support rod 2; a fixed positioning rod 202 is provided at the 0 scale position of the lamp bead support rod 2, and the fixed positioning rod 202 is connected to the detachable positioning rod 203;

[0049] The concave-disc-shaped slide 3 is provided with a concave disc 302 and a liquid collection groove 303. The concave disc 302 is used to hold the microbial liquid, and the liquid collection groove 303 is used to collect excess liquid. A slide positioning rod 301 is provided on one side of the concave-disc-shaped slide 3. The slide positioning rod 301 extends to the fixed positioning rod 202 of the lamp bead support rod 2. By comparing the upper surfaces of the slide positioning rod 301 and the fixed positioning rod 202 to determine whether the upper surface of the microbial liquid surface is aligned with the 0 scale mark of the lamp bead support rod 2;

[0050] Preferably, the area of ​​the concave disk 302 is the same as the effective detection area of ​​the UV irradiator used, but the maximum diameter does not exceed 1 cm; the depth of the concave disk 302 is not greater than 1 mm;

[0051] An irradiance test hole 101 is provided on the slide limiting platform 1. The irradiance test hole 101 is used to align the light emission center of the lamp bead with the center of the concave disk 302 on the concave disk-type slide 3. The size of the irradiance test hole 101 is consistent with the size of the UV radiometer used.

[0052] The lamp bead limiting fixture 4 is provided with a fixture limiting hole 402, a lamp bead limiting groove 403 on one side, and a fixture fixing port 401 on the other side; the lamp bead limiting fixture 4 is sleeved on the lamp bead support rod 2 through the fixture limiting hole 402 to prevent the lamp bead limiting fixture 4 from shaking left and right; after the lamp bead limiting fixture 4 is adjusted to a predetermined position along the lamp bead support rod 2, the lamp bead limiting fixture 4 is fixed to the lamp bead support rod 2 by locking the fixture fixing port 401; a test lamp bead is placed in the lamp bead limiting groove 403 to ensure that the position of the lamp bead remains consistent during each test;

[0053] When conducting an experiment to evaluate the microbial inactivation efficiency of ultraviolet lamp beads, first, the photosensitive surface of the ultraviolet radiometer is passed through the irradiance test hole 101, and the detachable positioning rod 203 is connected to the fixed positioning rod 202. Then, the scale height adjustment device 201 is used to make the 0 scale line of the scaled lamp bead support rod 2 and the upper surface of the ultraviolet radiometer on the same plane;

[0054] After removing the UV irradiator, remove the detachable positioning rod 203, place the concave disc slide 3 on the slide limiting platform 1, and adjust the height of the lamp bead support rod 2 so that the upper surface of the fixed positioning rod 202 is flush with the upper surface of the slide positioning rod 301. In this way, the upper surface of the slide concave disc 302 is aligned with the 0 scale position of the lamp bead support rod 2. At this time, the distance between the lamp bead and the upper surface of the microbial droplet is equal to the distance between the lamp bead and the photosensitive surface of the irradiator.

[0055] The purpose of the lamp bead support rod 2 is to keep the distance between the UV radiometer's photosensitive surface and the upper surface of the microbial droplet and the lamp bead consistent, thereby making the irradiance of the microbial liquid consistent with the test value of the UV radiometer.

[0056] The lamp bead limiting groove 403 and the glass slide limiting platform 1 are designed to align the lamp bead's luminous center with the irradiance test port at the center of the glass slide's concave disc 302. This aligns the UV irradiator's detection center and the center of the concave disc 302 with the lamp bead's luminous center to eliminate test errors caused by positional deviations between the lamp bead and the microbial droplets tested in different batches. After completing these steps, a slight excess of microbial liquid is dripped into the glass slide's concave disc 302 using a pipette, and the excess liquid is swept into the liquid collection tank 303 using a liquid leveler. The UV lamp can then be turned on for the microbial inactivation experiment. This ensures that the relative positions of the irradiator's photosensitive surface, the microbial liquid level, and the lamp bead's luminous surface remain consistent throughout each test.

[0057] The present invention provides a method for evaluating the sterilization efficiency of ultraviolet LED lamp beads, using the aforementioned device. The core concept of this device for lamp bead inactivation experiments is to strictly control the shape of microbial droplets, control the distance between the lamp bead and the microbial liquid surface, and strictly limit the microbial droplets and the lamp bead to the same vertical line, thereby eliminating the influence of various random factors on the microbial inactivation efficiency assessment.

[0058] The method specifically includes the following steps:

[0059] S1. Fix the UV irradiance meter photosensitive surface to the irradiance test port 101 of the slide limit platform 1, and use the fixed positioning rod 202 of the lamp support rod 2 to align the UV irradiance meter photosensitive surface with the 0 scale position of the lamp support rod 2;

[0060] S2. Adjust the lamp limit fixture 4 to the specified scale, turn on the lamp using the test current requirement, measure the lamp irradiance under experimental conditions and record it;

[0061] S3. The concave disc type slide 3 is placed on the slide limiting platform 1, and the slide positioning rod 301 is used to align the concave disc type slide 3 with the upper surface of the lamp support rod 2 at the 0 scale position;

[0062] S4. Align the center of the concave disc 302 with the center of the lamp bead; drop the microbial liquid into the concave disc 302, and use the flat plate to sweep the excess liquid into the liquid collection tank 303, and then safely handle the flat plate;

[0063] S5. Turn on the lamp beads to perform inactivation experiments;

[0064] S6. After the experiment is over, turn off the lamp beads and use a pipette to draw a certain amount of inactivated microbial liquid. Then, perform the culture or biological tissue infection experiment according to the normal procedure and compare it with the control group to confirm the microbial inactivation rate of the lamp beads. Safely dispose of the materials used, such as the concave disc-shaped slide 3, that have come into contact with the microbial droplets.

[0065] In a specific embodiment, in step S3, the detachable positioning rod 203 on the lamp bead support rod 2 is first removed, the concave disc type glass slide 3 is placed on the glass slide limiting platform 1, and the glass slide positioning rod 301 is used to make the upper surface of the concave disc type glass slide 3 flush with the upper surface of the fixed positioning rod 202.

[0066] In a specific embodiment, the flat plate is a disposable flat plate made of a hydrophobic material, the purpose of which is to unify the shape and thickness of the microbial droplets and prevent the random shape of the droplets from affecting the test results.

[0067] In a specific embodiment, the volume of the microbial liquid dripped into the concave dish 302 should be at least 5% larger than the volume of the concave dish 302 to ensure that the microbial liquid can fill the concave dish 302; the excess liquid is scraped into the liquid collection tank 303;

[0068] The liquid collection tank 303 is used in conjunction with a hydrophobic liquid leveling plate. When culture fluid is dripped into the slide concave dish 302, surface tension causes the liquid to form a convex shape. This shape is randomly affected by factors such as vibration, which affects the irradiance of the received ultraviolet light. Therefore, by dripping a slightly excessive amount of culture fluid, the convex portion can be smoothed out in conjunction with the liquid leveling plate, making it completely consistent in size and shape with the concave dish 302, eliminating experimental errors.

[0069] It should be understood that the various forms of the processes shown above can be used to reorder, add, or delete steps. For example, the steps described in the present disclosure can be performed in parallel, sequentially, or in a different order, as long as the desired results of the technical solutions disclosed in the present disclosure can be achieved. This is not limited herein.

[0070] The above specific embodiments do not limit the scope of protection of the present invention. Those skilled in the art will appreciate that various modifications, combinations, sub-combinations, and substitutions may be made based on design requirements and other factors. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention are intended to be included within the scope of protection of the present invention.

Claims

1. A UV LED lamp bead sterilization efficiency evaluation device, characterized in that: include: Slide limiting platform, lamp bead support rod, concave disc slide, lamp bead limiting fixture and platform support legs; The lamp bead support rod is vertically installed on the side of the slide limiting platform, and the lamp bead limiting fixture is installed on the lamp bead support rod and is horizontal to the slide limiting platform; the concave disc type slide is placed on the slide limiting platform; the platform support legs are installed under the slide limiting platform to support the slide limiting platform; The upper end of the lamp bead support rod is provided with a scale height adjustment device for adjusting the height of the lamp bead support rod; a fixed positioning rod is set at the 0 scale position of the lamp bead support rod; The slide limiting platform is provided with an irradiance test hole, which is used to align the luminous center of the lamp bead with the center of the concave disc slide; the size of the irradiance test hole is consistent with the UV radiometer used; The lamp bead limiting fixture is provided with a fixture limiting hole, a lamp bead limiting groove and a fixture fixing mouth, and is connected to the lamp bead support rod through the fixture limiting hole; after the lamp bead limiting fixture is adjusted to the predetermined position along the lamp bead support rod, the lamp bead limiting fixture is fixed to the lamp bead support rod by locking the fixture fixing mouth; the lamp bead limiting groove is used to place the test lamp bead to ensure that the position of the lamp bead is consistent for each test.

2. The UV LED lamp sterilization efficiency evaluation device according to claim 1, characterized in that: The concave disc type slide glass is provided with a concave disc and a liquid collecting groove. The concave disc is used to contain microbial liquid, and the liquid collecting groove is used to collect excess liquid.

3. The device for evaluating the sterilization efficiency of ultraviolet LED lamp beads according to claim 2, characterized in that: The area of ​​the concave disk is the same as the effective detection area of ​​the ultraviolet irradiator used, and the diameter of the concave disk does not exceed 1 cm and the depth does not exceed 1 mm.

4. A UV LED lamp sterilization efficiency evaluation device according to any one of claims 1 to 3, characterized in that: A slide positioning rod is provided on one side of the concave disc slide, and the slide positioning rod extends to the fixed positioning rod of the lamp bead support rod; by comparing whether the upper surface of the slide positioning rod is horizontal with the upper surface of the fixed positioning rod, it is determined whether the upper surface of the microbial liquid is aligned with the 0 scale of the lamp bead support rod.

5. The UV LED lamp sterilization efficiency evaluation device according to claim 1, characterized in that: The fixed positioning rod is connected to the detachable positioning rod; the detachable positioning rod is used to compare whether the 0 scale line of the lamp bead support rod and the upper surface of the ultraviolet irradiator are on the same plane.

6. The device for evaluating the sterilization efficiency of ultraviolet LED lamp beads according to claim 1, characterized in that: The sterilization is to inactivate viruses or bacteria.

7. A method for evaluating the sterilization efficiency of ultraviolet LED lamp beads, using the device according to any one of claims 1 to 5 for evaluation, characterized in that: The steps include: S1. Fix the UV irradiance meter's photosensitive surface to the irradiance test port on the slide limiter platform. Use the fixed positioning rod of the lamp support rod to align the UV irradiance meter's photosensitive surface with the 0 scale position of the lamp support rod. S2. Adjust the lamp limit fixture to the specified scale, turn on the lamp using the required test current, measure the lamp irradiance under the experimental conditions, and record it. S3. Place the concave disc slide on the slide limit platform and use the slide positioning rod to align the concave disc slide upper surface with the 0 scale position of the lamp support rod; S4. Align the center of the concave disc with the center of the lamp bead; Drop the microbial liquid into the concave dish and use a flat plate to remove excess liquid; S5. Turn on the lamp beads to perform the inactivation experiment; S6. After the experiment is over, turn off the lamp beads, draw a certain amount of inactivated microbial liquid, and then perform culture or biological tissue infection experiments to confirm the microbial inactivation rate of the lamp beads; safely dispose of materials that have come into contact with the microbial liquid.

8. The method for evaluating the sterilization efficiency of ultraviolet LED lamp beads according to claim 7, wherein: In step S4, the volume of the microbial liquid dripped into the concave dish is at least 5% larger than the volume of the concave dish, ensuring that the microbial liquid fills the concave dish; the excess liquid is scraped into the liquid collection tank of the concave dish slide.

9. The method for evaluating the sterilization efficiency of ultraviolet LED lamp beads according to claim 7, wherein: In step S3, the detachable positioning rod on the lamp bead support rod is first removed, the concave disc type slide is placed on the slide limiting platform, and the slide positioning rod is used to make the upper surface of the concave disc type slide flush with the upper surface of the fixed positioning rod.

10. The method for evaluating the sterilization efficiency of ultraviolet LED lamp beads according to claim 7, wherein: The flat liquid plate is a disposable flat liquid plate made of a hydrophobic material.

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