Model for monitoring Alzheimer's disease cell cycl-G2 / M period and construction method and application thereof
CN119534849APending Publication Date: 2025-02-28ZHENJIANG NO 4 PEOPLES HOSPITAL +2
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Patent Information
- Application Number
- CN202410452106.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-16
- Publication Date
- 2025-02-28
AI Technical Summary
Technical Problem
The prior art is difficult to effectively monitor and regulate G2/M phase block of neuronal cell cycle in Alzheimer's disease, making the treatment difficult to work.
Method used
APP/PS1 dual transgenic mice were hybridized with Fucci-Green (G2/M phase) reporter mice through gene recombination technology, and a G2/M phase-Fucci-Green reporter mouse model was constructed in the Alzheimer's disease cell cycle, and the number of cells in the G2/M phase was detected by immunofluorescence technology.
Benefits of technology
It has achieved efficient monitoring of the number of cells in the G2/M phase of the cell cycle of Alzheimer's disease, provided an important tool for screening therapeutic drugs and studying disease mechanisms, and is of good practicality.
✦ Generated by Eureka AI based on patent content.
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Abstract
The invention provides a model for monitoring Alzheimer's disease cell cycl-G2 / M period as well as a construction method and application thereof, and belongs to the technical field of biology. According to the invention, an APP / PS1 double transgenic mouse and a Fucci-Green (G2 / M stage) reporter gene mouse are hybridized through a gene recombination technology to obtain an Alzheimer's disease cell cycle G2 / M stage-Fucci-Green reporter gene mouse APP / PS1:: mAG-hGem( 1 / 110), namely a model mouse for monitoring the Alzheimer's disease cell cycle G2 / M stage; the model mouse for monitoring the cell cycle of the Alzheimer's disease can be used for screening Alzheimer's disease treatment drugs, has important significance on mechanism research of the Alzheimer's disease and targeted drug development, and has good practicability.
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