Bamboo and rattan associated forest carbon sink enhancing method based on biotechnology

Abstract

The invention relates to the technical field of ecological engineering, and discloses a bamboo and rattan associated forest carbon sink enhancing and stabilizing method based on biotechnology, which comprises the following steps: sequentially applying a soil conditioner, a PGPR preparation and a mycorrhizal fungus agent to soil, and planting bamboo and rattan plants in the soil; wherein the microorganisms in the PGPR preparation comprise streptomyces ferulae and bacillus mucilaginosus; microorganisms in the mycorrhizal fungi fungicide comprise hypocrea rubrofusca and globosporium albus. According to the bamboo and rattan associated forest carbon sink enhancing and stabilizing method, through scientific and reasonable soil improvement and microbial preparation application, the plant growth environment is optimized, the stable carbon storage capacity of plants and soil is improved, and therefore the carbon sink of the bamboo and rattan associated forest is obviously enhanced and stabilized.

Description

Technical Field

[0001] The invention relates to the technical field of ecological engineering, and in particular to a method for enhancing and stabilizing carbon sinks in bamboo and rattan associated forests based on biotechnology. Background Art

[0002] In the context of global climate change, the enhancement and stabilization of carbon sinks has become one of the important means to address climate change. As an ecosystem with a high carbon storage capacity, bamboo and rattan forests have unique advantages in carbon sink function. However, traditional bamboo and rattan forest management methods often ignore the potential of biotechnology in improving carbon sink capacity. Existing methods mainly focus on physical and chemical means, such as soil improvement and water management, while the application of biotechnology is relatively small.

[0003] The application of biotechnology in agriculture and forestry has achieved remarkable results, such as improving soil quality through microbial technology. However, the application of these technologies to enhance and stabilize carbon sinks in bamboo and rattan forests is still a relatively new research field. Summary of the invention

[0004] In view of this, the present invention proposes a method for enhancing and stabilizing carbon sinks in bamboo and rattan associated forests based on biotechnology, aiming to improve the carbon stable storage capacity of bamboo and rattan associated forests through biotechnology means, thereby enhancing and stabilizing its carbon sink function.

[0005] The present invention proposes a method for enhancing and stabilizing carbon sinks in bamboo and rattan associated forests based on biotechnology, comprising:

[0006] After applying soil conditioner, PGPR preparation and mycorrhizal fungi agent to the soil in sequence, bamboo and rattan plants were planted in the soil;

[0007] Wherein, the microorganisms in the PGPR preparation include Bacillus subtilis and Bacillus colloids;

[0008] The microorganisms in the mycorrhizal fungal agent include Hypocrea jecorina and White Spherical Cyst.

[0009] Preferably, the soil conditioner comprises biochar, organic fertilizer, mineral additives and water retaining agent.

[0010] Preferably, the mineral additives are bentonite and vermiculite;

[0011] And / or, the water retaining agent is potassium polyacrylate.

[0012] Preferably, the preparation method of the PGPR preparation comprises:

[0013] The fermentation broths of Streptomyces ferulae and Bacillus mucilaginosus are mixed, and then stabilizers and preservatives are added to obtain the PGPR preparation.

[0014] Preferably, the culture medium of Streptomyces ferulae comprises glucose, yeast extract, and inorganic salts;

[0015] And / or, the culture medium of the Bacillus subtilis includes sucrose, peptone and sodium chloride.

[0016] Preferably, the culture conditions of Hypocrea jecorina and Sphaerocystis albus include:

[0017] The temperature is 30±3°C, the pH value is 7.0±0.2, the shaking speed is 150-200 revolutions per minute, and the culture time is between 24-48 hours.

[0018] Preferably, the preparation method of the mycorrhizal fungal agent comprises:

[0019] After Hypocrea jecorina and White Spherical Sporangium are cultured separately, the mycelia are collected and mixed, and a stabilizer and a preservative are added to obtain the mycorrhizal fungal agent.

[0020] Preferably, the culture medium of Hypocrea jecorina is PDA culture medium;

[0021] And / or, the culture medium of the white spherical cysts is MMM culture medium.

[0022] Preferably, the culture conditions of Hypocrea jecorina and Sphaerocystis albus include:

[0023] The temperature is 25±2°C, the pH is 6.0±0.2, the culture method is static culture, and the culture time is 8-14 days.

[0024] Preferably, the bamboo and rattan plants are root pruned before planting; the root pruning is performed in a manner of retaining the main root and removing the lateral roots.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] 1. It improves the carbon sequestration capacity of the soil. The use of soil conditioners such as biochar increases the organic matter content of the soil, while the symbiosis between mycorrhizal fungi and plant roots can promote the accumulation of organic carbon in the soil, thereby enhancing the carbon sink function of the soil.

[0027] 2. The growth rate and biomass of bamboo and rattan plants have been improved. By applying soil conditioners, PGPR preparations and mycorrhizal fungi agents, the soil fertility and microbial environment have been improved, thereby promoting the growth and absorption capacity of plant roots and increasing the plant's ability to absorb nutrients and water.

[0028] 3. Enhanced the stress resistance of bamboo and rattan plants. The microorganisms in PGPR preparations can produce plant growth hormones and stress resistance substances, helping plants resist diseases and environmental pressures, and improving plant survival rates and growth stability.

[0029] 4. Optimized resource utilization efficiency. The present invention reduces the use of chemical fertilizers and pesticides and reduces production costs through scientifically proportioned microbial preparations and improvers, while increasing the yield and quality of bamboo and rattan plants, achieving a win-win situation for ecology and economy.

[0030] In summary, the present invention provides an efficient, environmentally friendly and sustainable method for enhancing and stabilizing carbon sinks in bamboo and rattan associated forests, which can not only improve the growth and yield of bamboo and rattan plants, but also effectively enhance the carbon sequestration capacity of the soil, providing a new technical approach for responding to climate change and protecting the ecological environment. DETAILED DESCRIPTION

[0031] The exemplary embodiments of the present disclosure will be described in more detail below. Although the exemplary embodiments of the present disclosure are shown below, it should be understood that the present disclosure can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided in order to enable a more thorough understanding of the present disclosure and to be able to fully convey the scope of the present disclosure to those skilled in the art. It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other. The present invention will be described in detail below in conjunction with the embodiments.

[0032] Example 1 Preparation of microbial preparations

[0033] 1. Preparation of PGPR preparations

[0034] 1. Microorganisms

[0035] Streptomyces ferulae NRRL 8165, Paenibacillus sp. Bio-29306.

[0036] 2. Culture medium formula

[0037] Streptomyces ferulae: glucose 20 g / L, yeast extract 5 g / L, inorganic salt solution 10 mL / L.

[0038] Bacillus subtilis: sucrose 15g / L, peptone 10g / L, sodium chloride 5g / L.

[0039] 3. Culture conditions

[0040] Temperature: 30℃; pH: 7.0; Shaking speed: 150rpm; Cultivation time: 24-48 hours. During the cultivation process, the growth of microorganisms and the content of metabolites are regularly tested to ensure the best fermentation effect.

[0041] 4. Fermentation broth treatment

[0042] After the fermentation, the fermentation broth of each microorganism was centrifuged at 8000 rpm for 10 minutes to remove the bacteria and collect the supernatant. Then, the supernatants of different microorganisms were mixed in a ratio of 1:1, and 3% of stabilizer (kaolin) and 0.2% of preservative (sodium benzoate) were added to improve the storage stability of the preparation.

[0043] 5. Drying and molding of preparations

[0044] The mixed liquid is made into a powdered preparation by spray drying. The drying conditions are an inlet temperature of 130°C and an outlet temperature of 70°C. The obtained PGPR preparation has good fluidity, solubility and stability, and is suitable for rhizosphere application of bamboo and rattan plants, which can significantly improve the growth rate and disease resistance of crops.

[0045] 2. Preparation of mycorrhizal fungi preparations

[0046] 1. Use mycorrhizal fungi

[0047] Hypocrea rubra YSRI B IO-J1209 and Sphaerocystis albus YIM 48782T.

[0048] 2. Culture medium formula

[0049] Hypocrea jecorina: PDA medium.

[0050] White spherical cysts: MMM medium.

[0051] 3. Culture conditions

[0052] Temperature: 25℃; pH: 6.0; Cultivation method: static culture; Cultivation time: 14 days. During the cultivation process, the growth and infection ability of mycorrhizal fungi were regularly tested to ensure the best cultivation effect.

[0053] 4. Mycelium treatment

[0054] After the culture is completed, the mycelia of the mycorrhizal fungi are collected by filtering through gauze, and the culture medium residue is removed. The mycelia of different mycorrhizal fungi are mixed in a ratio of 1:1, and 3% of a stabilizer and 0.2% of a preservative are added to improve the storage stability of the preparation.

[0055] 5. Drying and molding of preparations

[0056] The mixture is made into a powdered preparation by freeze drying. The drying conditions are pre-freezing at -40°C and a vacuum degree of 0.05 mbar. The obtained mycorrhizal fungus preparation has good fluidity, solubility and stability, is suitable for rhizosphere application of bamboo and rattan plants, and can significantly improve the absorption capacity and stress resistance of plants.

[0057] 3. Verify the effect of mycorrhizal fungi preparations

[0058] In order to verify the actual effect of mycorrhizal fungi preparations, field trials and greenhouse trials were designed. Field trials were conducted in bamboo and rattan plant planting areas in different regions to evaluate the application effect of the preparations under different environmental conditions. Greenhouse trials were conducted under controlled conditions to observe and record data more accurately.

[0059] Bambusa oldhamii Munro and Daemonorops jenkinsiana (Gr iff.) Mart. were selected as the experimental bamboo and rattan plant species. The experimental group was fertilized with mycorrhizal fungi preparations, while the control group was fertilized with conventional fertilizers without any mycorrhizal fungi preparations.

[0060] The mycorrhizal fungi preparation was mixed with the soil thoroughly and applied at a rate of 500 grams per mu. The application time was selected at the early stage of plant growth to ensure that the preparation could fully exert its effect. The control group was given an equal amount of conventional fertilizer.

[0061] During the experiment, plant growth indicators were measured regularly, including plant height, stem diameter, number of leaves and leaf area. At the same time, the root development of the plants was tested, such as root length, root weight and root activity. In addition, the disease resistance and stress resistance of the plants were recorded, such as resistance to drought, salinity and disease.

[0062] The differences between the experimental group and the control group were compared by statistical analysis. Analysis of variance (ANOVA) was used to test the significant differences of the data of each group, and multiple comparison methods (such as LSD method) were used to determine the specific differences between different treatments.

[0063] The test results showed that the bamboo and rattan plants treated with mycorrhizal fungi preparations were significantly better than the control group in terms of growth rate, root development and stress resistance. Specifically, the plants were taller, the stems were thicker, the number of leaves increased, the root system was more developed, and the resistance to diseases and adversity was stronger. These results show that mycorrhizal fungi preparations can significantly improve the growth performance and health of bamboo and rattan plants, and have important application value and promotion potential.

[0064] 4. Economic Benefit Analysis

[0065] In order to evaluate the economic benefits of mycorrhizal fungi preparations in actual production, the yield and quality of the experimental group and the control group were recorded and analyzed in detail. By comparing the yield, quality and input cost of the two groups of plants, the economic benefits of applying mycorrhizal fungi preparations were calculated.

[0066] The results showed that the plants treated with mycorrhizal fungi preparations not only had higher yields, but also improved quality. For example, the bamboo shoot yield of green bamboo increased by 15.3%, and the number of inflorescences and flowering period of yellow rattan were extended, improving the ornamental and economic value. In addition, because the preparation can improve the stress resistance of plants, it reduces the use of pesticides and irrigation, further reducing production costs.

[0067] V. Environmental Impact Assessment

[0068] In addition to economic benefits, the impact of mycorrhizal fungi preparations on the environment is also an important aspect of evaluation. By monitoring the changes in the physical and chemical properties of the soil before and after the application of the preparations, the long-term impact of the preparations on soil health can be evaluated.

[0069] Studies have found that mycorrhizal fungi preparations can improve soil structure, increase soil organic matter content, and improve soil microbial diversity. These changes contribute to the sustainable use of soil, enhance the self-repairing ability of soil, reduce dependence on chemical fertilizers and pesticides, and thus reduce pollution to the environment.

[0070] In addition, the use of mycorrhizal fungi preparations also promotes the absorption of carbon dioxide by bamboo and rattan plants, increases the biomass of bamboo and rattan plants and the stability of soil organic carbon, and thus helps to mitigate the greenhouse effect. Therefore, mycorrhizal fungi preparations not only have a positive impact on soil carbon storage and bamboo and rattan plant productivity, but also have important significance for environmental protection and ecological balance.

[0071] In summary, mycorrhizal fungal preparations show significant advantages in improving the growth performance of bamboo and rattan plants, increasing soil carbon storage and its stability, increasing economic benefits and improving the environment.

[0072] Example 2 Preparation of soil conditioner

[0073] 1. Material preparation

[0074] Preparation of biochar: Use bamboo chips or rice husks as raw materials, wash and dry them until the moisture content is less than 10%. Use a crusher to crush the dried bamboo chips or rice husks to a particle size of 0.5-1 cm, remove particles larger than 1 cm, put them into a rotary furnace, and perform anoxic pyrolysis at 550°C for 2 hours with an oxygen supply of 10%; after the pyrolysis is completed, wait for the temperature in the furnace to naturally drop to room temperature, and then take out the biochar.

[0075] Organic fertilizer fermentation: The farmyard manure is composted and fermented to ensure that its maturity reaches more than 60% to reduce harmful bacteria and insect eggs. During the fermentation period, the temperature is controlled at 55±2℃, and the pile is turned regularly to maintain ventilation. The fermentation time is 40 days.

[0076] The volume ratio of biochar, organic fertilizer and mineral additives is 7:5:4; the amount of water retainer added is 3% of the total weight of biochar, organic fertilizer and mineral additives. Among them, the mineral additive is a mixture of bentonite and vermiculite, and the volume ratio of bentonite and vermiculite is 2:1; the biochar is made of bamboo scraps or rice husks, and is prepared by pyrolysis under low oxygen conditions at 550℃; the organic fertilizer is decomposed farmyard manure; the water retainer is potassium polyacrylate.

[0077] 2. Preparation process

[0078] During the preparation process, first mix the bentonite and vermiculite in proportion, then add the biochar and organic fertilizer, and stir thoroughly to ensure that the various ingredients are evenly distributed. Then, mix the mineral additives with the biochar and organic fertilizer mixture evenly, and finally add the water retaining agent and continue stirring until it is completely mixed. The entire mixing process should be carried out under well-ventilated conditions to avoid dust from affecting the operator.

[0079] 3. Verify the effect of soil conditioners

[0080] In order to verify the actual effect of soil conditioners, field tests and greenhouse tests were designed. Field tests were conducted on different types of soil to evaluate the application effect of conditioners under different soil conditions. Greenhouse tests were conducted under controlled conditions to observe and record data more accurately.

[0081] Three different types of soil were selected: sandy soil, loam and clay. Soil conditioners were applied to the test group, while no conditioner was applied to the control group. Soil conditioners were evenly applied to the test group soil at a rate of 500 ± 10 g per square meter. The application time was chosen at the early growth stage of the plant (wheat) to ensure that the conditioner could fully function. The control group remained as it was without any treatment.

[0082] During the experiment, the physical and chemical properties of the soil were measured regularly, including pH value, organic matter content, nitrogen, phosphorus and potassium content, soil compactness and water retention capacity. At the same time, the growth of wheat was observed, including plant height, stem diameter, number of leaves and leaf area. In addition, the disease resistance and stress resistance of wheat (resistance to drought, salinity and disease) were recorded.

[0083] The differences between the experimental group and the control group were compared by statistical analysis. The variance analysis was used to test the significant differences of the data of each group, and the specific differences between different treatments were determined by multiple comparison methods (such as LSD method).

[0084] The test results showed that the soil treated with soil conditioners was significantly better than the control group in terms of physical and chemical properties and plant growth indicators. Specifically, the soil pH value was closer to neutral, the organic matter content increased significantly, the content of nutrients such as nitrogen, phosphorus and potassium increased, the soil compactness decreased, and the water retention capacity increased. The growth rate of wheat was accelerated, the plant height, stem thickness, leaf number and leaf area increased, and the disease resistance and stress resistance were also better.

[0085] These results show that soil conditioners can effectively improve soil structure and nutritional status, promote healthy growth of wheat, and have significant agricultural application value. The application of conditioners not only improves wheat yield and quality, but also reduces the use of fertilizers and pesticides, thereby reducing production costs and environmental pollution.

[0086] In summary, soil conditioners have shown significant advantages in improving soil quality, promoting wheat growth, increasing economic benefits and protecting the environment. They have broad application prospects and provide strong support for achieving sustainable agricultural development.

[0087] Example 3 Method for enhancing and stabilizing carbon sinks in bamboo and rattan associated forests

[0088] 1. Selection and preparation of bamboo and rattan plants

[0089] When choosing bamboo plants, green bamboo should be given priority. The root system of green bamboo should be properly pruned, retaining the main root and removing the lateral roots to facilitate the growth of the main root and improve its absorption function. Be sure to choose healthy plants that are free of pests and diseases to ensure that they have excellent growth potential.

[0090] 2. Soil improvement and application of microbial preparations

[0091] On the basis of soil improvement, the prepared plant growth promoting bacteria (PGPR) preparation and mycorrhizal fungi preparation should be evenly applied to the soil according to the recommended dosage. The specific operation steps are as follows:

[0092] 1. Application of soil conditioner: Spread the soil conditioner evenly on the soil surface at a dosage of 500±10 grams per mu, and perform deep tillage to promote full mixing of the conditioner and soil.

[0093] 2. Application of PGPR preparation: After applying the soil conditioner, mix the PGPR preparation with an appropriate amount of water at a dosage of 130±10 grams per mu of land, spray it evenly on the soil surface, and perform shallow tillage to ensure that the preparation is fully integrated with the soil.

[0094] 3. Application of mycorrhizal fungi preparations: After applying the PGPR preparation, mix the mycorrhizal fungi preparation with an appropriate amount of water at a dosage of 180±10 grams per mu of land, apply it directly into the soil, and perform shallow tillage to ensure that the mycorrhizal fungi preparation is in contact with the plant roots.

[0095] After applying microbial preparations and soil conditioners, appropriate irrigation should be carried out to promote the effectiveness of the preparations.

[0096] 3. Planting and management of bamboo and rattan plants

[0097] 1. Planting time: It is advisable to choose spring or autumn for planting. The climatic conditions in these two seasons are conducive to the growth and survival of bamboo and rattan plants.

[0098] 2. Planting density: According to the growth characteristics of green bamboo, the planting density is reasonably arranged, usually 130 plants are planted per acre to ensure that there is enough growth space between plants.

[0099] 3. Fertilization management: During the growth period of the plants, organic and inorganic fertilizers should be applied in a timely manner according to the soil fertility and the growth conditions of the plants. When applying fertilizers, avoid direct contact between the fertilizers and the plant roots to prevent root burn.

[0100] 4. Pest and disease control: Regularly check the growth of plants, and promptly detect and deal with pest and disease problems. For common pests and diseases, a combination of biological control and chemical control can be used for prevention and control.

[0101] 5. Pruning management: During the growth of the plant, prune it in a timely manner to remove dead branches, diseased branches and overly dense branches to promote ventilation and light transmission of the plant and improve the growth quality and yield of the plant.

[0102] Through the above methods, the carbon sequestration capacity of bamboo and rattan companion forests can be effectively improved, the healthy growth of bamboo and rattan plants can be promoted, and soil fertility can be enhanced, achieving both ecological and economic benefits.

[0103] 4. Carbon sink enhancement verification method and results

[0104] In order to verify the effect of the carbon sink enhancement and stabilization method of bamboo and rattan associated forests, the present invention designed a series of monitoring and evaluation schemes. First, by regularly measuring the biomass of bamboo and rattan plants, including the dry weight of the aboveground and underground parts, the changes in their carbon stable storage capacity were evaluated. In addition, the soil carbon storage determination method was used to determine the organic carbon content in the soil to evaluate the effects of soil improvement and the application of microbial preparations on the stable storage of soil carbon.

[0105] 1. Determination of bamboo and rattan plant biomass: At different growth stages, randomly select bamboo and rattan plants, harvest and dry their above-ground and underground parts, and measure their dry weight. By comparing the changes in plant biomass before and after improvement, the carbon sink enhancement and stabilization effects can be evaluated.

[0106] 2. Determination of soil stable organic carbon content: Soil samples were collected at different locations in the test site to determine their stable organic carbon content. By comparing the changes in soil stable organic carbon content before and after improvement, the effects of soil conditioners and microbial preparations on the stable storage capacity of soil carbon were evaluated.

[0107] 3. Comprehensive evaluation of carbon sink enhancement and stabilization effects: Combining the results of bamboo and rattan plant biomass determination and soil stable organic carbon content determination, the carbon balance model is used to calculate the carbon sink capacity of the entire bamboo and rattan companion forest system. By comparing the changes in carbon sink capacity before and after improvement, the effect of carbon sink enhancement methods is comprehensively evaluated. Among them, the carbon balance model is a calculation tool based on the principle of ecosystem carbon cycle, which can simulate and predict the impact of different management measures on carbon sink capacity. The model takes into account multiple carbon cycle processes such as photosynthesis, respiration, soil organic carbon decomposition and vegetation growth. By inputting vegetation growth data before and after improvement, soil physical and chemical properties change data, and climate conditions change data, the model can output changes in carbon sink capacity before and after improvement.

[0108] In order to ensure the accuracy of the evaluation results, the present invention also carried out long-term field monitoring, recording the growth rate of bamboo and rattan plants, biomass accumulation, and dynamic changes in soil stability organic carbon content. By comparing the model prediction results with the actual monitoring data, the carbon balance model was calibrated and verified to ensure that it can accurately reflect the carbon sequestration capacity of the bamboo and rattan associated forest system.

[0109] The comprehensive assessment results show that the carbon sequestration capacity of bamboo and rattan forests has been significantly improved through measures such as improving soil structure, increasing organic matter input and optimizing bamboo and rattan planting structure. After the improvement, the biomass of bamboo and rattan plants increased by about 20%, and the soil stable organic carbon content also increased by more than 10%. This shows that through scientific and reasonable management measures, the bamboo and rattan forest system has huge carbon sequestration potential and can play an important role in responding to climate change.

[0110] In summary, through scientific and reasonable soil improvement and application of microbial agents, the carbon sink of bamboo and rattan companion forests can be effectively enhanced and stabilized, the carbon cycle of the ecosystem can be promoted, and strong support can be provided for responding to climate change and protecting the ecological environment.

[0111] 5. Principle and Mechanism Analysis

[0112] The principle mechanism of carbon sequestration enhancement method of bamboo and rattan associated forest mainly involves the following aspects:

[0113] 1. The role of plant growth promoting bacteria (PGPR): The PGPR of the present invention is a group of beneficial microorganisms that can coexist with plant roots and promote plant growth. They promote plant growth through a variety of mechanisms, including by producing plant hormones, fixing nitrogen, dissolving phosphorus and other nutrients, and inhibiting the growth of pathogenic microorganisms. The application of PGPR can significantly increase the biomass of plants, thereby increasing carbon storage capacity. Among them, the synergistic mechanism of Streptomyces ferulae and Bacillus colloids is that they can form a complex microbial network that acts together on the plant rhizosphere microenvironment. Streptomyces ferulae can produce antibiotics and enzymes to inhibit the growth of pathogens and promote the synthesis of plant growth hormones. Bacillus colloids can convert nitrogen in the atmosphere into a form that can be absorbed by plants, thereby reducing the use of chemical fertilizers. For example, under harsh conditions such as drought and salinity, PGPR can help plants better absorb water and nutrients and improve their survival rate. In addition, the use of PGPR also helps to reduce the use of chemical pesticides and fertilizers, thereby reducing the negative impact of agricultural production on the environment and achieving the development of sustainable agriculture.

[0114] 2. Symbiotic effect of mycorrhizal fungi: The mycorrhizal fungi of the present invention form a symbiotic relationship with the plant root system, and by expanding the absorption area of ​​the plant root system, the plant's absorption capacity for water and nutrients is improved. Mycorrhizal fungi can also enhance the resistance of plants to diseases and promote healthy growth of plants. The application of mycorrhizal fungi helps to improve the carbon fixation efficiency of plants, thereby enhancing and stabilizing the carbon sink capacity. Among them, the synergistic mechanism of rubropoda and white spherical cysts lies in their unique biological characteristics. Rubropoda can decompose organic matter, provide additional carbon sources and nutrients for plants, and improve soil structure at the same time, and promote the diversity and activity of soil microorganisms. White spherical cysts have a strong root expansion ability, can be closely integrated with the plant root system, form a huge root network, and significantly improve the plant's absorption efficiency of water and mineral elements. This synergistic effect not only enhances the growth potential of plants, but also improves the adaptability of plants to adverse environments, such as drought, salinity, etc., thereby further enhancing the carbon sink effect.

[0115] 3. The role of soil conditioners: Soil conditioners are chemical substances or biological agents widely used in agriculture and horticulture. Their main purpose is to optimize the physical, chemical and biological properties of the soil to create an environment more conducive to plant growth. By using soil conditioners, the structure of the soil can be significantly improved, making it more loose and breathable, which is conducive to the extension of plant roots and the absorption of nutrients. At the same time, these conditioners can also effectively increase the organic matter content in the soil. Organic matter is an important component of soil fertility and plays a vital role in soil water retention, nutrient circulation and microbial activity. In addition to directly improving the physical and chemical properties of the soil, soil conditioners can also indirectly improve soil quality by affecting the soil microbial environment. They provide a suitable living environment for microorganisms in the soil and promote the growth and reproduction of beneficial microorganisms. These microorganisms play an important role in decomposing organic matter, converting nutrients and inhibiting pathogens, thereby enhancing the carbon storage capacity of the soil and helping to maintain the long-term health and productivity of the soil. In summary, soil conditioners have significant effects in improving soil structure, increasing organic matter content, improving water and fertilizer retention capacity, and optimizing the microbial environment. They are one of the important means for the sustainable development of modern agriculture.

[0116] 4. Carbon sequestration capacity of bamboo and rattan plants: Bamboo and rattan plants have a high growth rate and biomass accumulation capacity, and can quickly absorb carbon dioxide from the atmosphere and fix it in the plant body. Through reasonable planting and management, the carbon sequestration efficiency of bamboo and rattan plants can be further improved, thereby enhancing and stabilizing the carbon sink of the entire associated forest ecosystem.

[0117] 5. Optimization of ecosystem carbon cycle: Through scientific and reasonable soil improvement and application of microbial agents, the carbon cycle process of the bamboo and rattan forest ecosystem can be optimized. Soil improvement and application of microbial agents can improve soil stability and enhance the soil's carbon storage capacity, while promoting the healthy growth of plants and improving the carbon fixation efficiency of plants. Through these measures, the carbon sequestration capacity of the bamboo and rattan forest system can be maximized.

[0118] In summary, the carbon sink enhancement and stabilization method of bamboo and rattan associated forests of the present invention optimizes the plant growth environment and improves the carbon storage capacity of plants and soil through scientific and reasonable soil improvement and application of microbial preparations, thereby significantly enhancing the carbon sink capacity of bamboo and rattan associated forests. This method not only helps to cope with climate change, but also promotes the sustainable development of ecosystems.

[0119] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in the relevant field should understand that the specific implementation methods of the present invention can still be modified or replaced by equivalents. Any modification or equivalent replacement that does not depart from the spirit and scope of the present invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. A method for enhancing and stabilizing carbon sinks in bamboo and rattan associated forests based on biotechnology, characterized in that: include: After applying soil conditioner, PGPR preparation and mycorrhizal fungi agent to the soil in sequence, bamboo and rattan plants were planted in the soil; Wherein, the microorganisms in the PGPR preparation include Streptomyces ferulae and Bacillus mucilaginosus; The microorganisms in the mycorrhizal fungal agent include Hypocrea jecorina and White Spherical Cyst.

2. The method for enhancing and stabilizing carbon sinks in bamboo and rattan associated forests based on biotechnology according to claim 1, characterized in that: The soil conditioner includes biochar, organic fertilizer, mineral additives and water retaining agent.

3. The method for enhancing and stabilizing carbon sinks in bamboo and rattan associated forests based on biotechnology according to claim 2, characterized in that: The mineral additives are bentonite and vermiculite; And / or, the water retaining agent is potassium polyacrylate.

4. The method for enhancing and stabilizing carbon sinks in bamboo and rattan associated forests based on biotechnology according to claim 1, characterized in that: The preparation method of the PGPR preparation comprises: The fermentation broths of Streptomyces ferulae and Bacillus mucilaginosus are mixed, and then stabilizers and preservatives are added to obtain the PGPR preparation.

5. The method for enhancing and stabilizing carbon sinks in bamboo and rattan associated forests based on biotechnology according to claim 4, characterized in that: The culture medium of Streptomyces ferulae includes glucose, yeast extract and inorganic salts; And / or, the culture medium of the Bacillus subtilis includes sucrose, peptone and sodium chloride.

6. The method for enhancing and stabilizing carbon sinks in bamboo and rattan associated forests based on biotechnology according to claim 4, characterized in that: The culture conditions of the streptomyces ferulae and the bacillus colloids include: The temperature is 30±3°C, the pH value is 7.0±0.2, the shaking speed is 150-200 revolutions per minute, and the culture time is between 24-48 hours.

7. The method for enhancing and stabilizing carbon sinks in bamboo and rattan associated forests based on biotechnology according to claim 1, characterized in that: The preparation method of the mycorrhizal fungal agent comprises: After Hypocrea jecorina and White Spherical Sporangium are cultured separately, the mycelia are collected and mixed, and a stabilizer and a preservative are added to obtain the mycorrhizal fungal agent.

8. The method for enhancing and stabilizing carbon sinks in bamboo and rattan associated forests based on biotechnology according to claim 7, characterized in that: The culture medium of the Hypocrea jecorina is PDA culture medium; And / or, the culture medium of the white spherical cysts is MMM culture medium.

9. The method for enhancing and stabilizing carbon sinks in bamboo and rattan associated forests based on biotechnology according to claim 7, characterized in that: The culture conditions of the jecorina fungus and the white spherical cyst fungus include: The temperature is 25±2°C, the pH is 6.0±0.2, the culture method is static culture, and the culture time is 8-14 days.

10. The method for enhancing and stabilizing carbon sinks in bamboo and rattan associated forests based on biotechnology according to claim 1, characterized in that: The bamboo and rattan plants are root pruned before planting; The root system is pruned in a manner of retaining the main root and removing the lateral roots.