Application of isoalantolactone as pesticide synergist in aphid control

Abstract

The invention relates to application of isoalantolactone or a derivative thereof as a pesticide synergist in aphid prevention and control, low-concentration isoalantolactone has a remarkable synergistic effect on chemical pesticides, 2 mu g / ml isoalantolactone and 25% thiamethoxam are mixed for use, the lethal medium concentration of thiamethoxam on four aphids can be remarkably reduced, and the killing effect of the thiamethoxam on the aphids is remarkably improved. The insecticidal activity of thiamethoxam on aphids is improved. Field efficacy tests also show that after 50% recommended dosage of 48% clothianidin or 22% sulfoxaflor is mixed with 3 [mu] g / ml of isoalantolactone for use, the insect decline rate and the control effect on corn aphids and rhopalosiphum padi are equivalent to those of 100% recommended dosage of the 48% clothianidin or 22% sulfoxaflor respectively, and after the 48% clothianidin or 22% sulfoxaflor is mixed with 3 [mu] g / ml of isoalantolactone. Therefore, the isoalantolactone not only remarkably improves the field insecticidal activity of the pesticide to the corn aphid and the rhopalosiphum padi, but also reduces the pesticide consumption by 50%, achieves the purposes of pesticide reduction and synergism, and has a positive effect on comprehensive prevention and control of field pests.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticide synergists, and in particular to the application of isoinulin lactone or its derivatives as pesticide synergists in aphid control. Background Art

[0002] Aphids mainly gather on the back of leaves, tender stems and other tender parts of plants as adults and nymphs, sucking sap, causing the leaves to turn yellow, curl and deform, grow poorly, and young leaves to be deformed. The large amount of molting produced by aphids during their growth and their excrement (honeydew) can induce sooty mold, directly affecting the photosynthesis of the plants, causing slow growth or death of the plants.

[0003] Chemical control is currently the most commonly used method. This method is quick and suitable for large-scale use. Commonly used pesticides include thiamethoxam, fluphenazine, clothianidin, imidacloprid, pirimicarb, cypermethrin, fenvalerate, abamectin, and fenitrothion.

[0004] However, long-term use of a single drug can easily lead to drug resistance. On the other hand, sufficient liquid medicine must be applied to ensure the prevention and control effect, with a focus on spraying the back of leaves, young leaves, young buds, young shoots, etc. of crops, which requires a large amount of liquid medicine.

[0005] Therefore, it is of great significance to find effective synergists to improve the effectiveness of pesticides in controlling aphids while reducing the dosage of pesticides.

[0006] Inula is a perennial herb belonging to the genus Inula of the Asteraceae family. Its dried root can be used for medicinal purposes. It is bitter and warm in nature. It has the effects of strengthening the spleen and stomach, promoting qi and relieving pain, calming the fetus, and antibacterial. Isoalantolactone is a sesquiterpene lactone compound present in Inula. It is one of the main active ingredients of Inula, and has the effects of expelling ascarids, antiprotozoa, and antibacterial. However, there has been no report on the research and application of isoalantolactone in the integrated management of agricultural pests. Summary of the invention

[0007] Based on this, the present invention provides an application of isoinulin lactone or its derivatives as pesticide synergists in aphid control. Mixing isoinulin lactone or its derivatives with pesticides can enhance the control effect of the agent and reduce the amount of the agent used.

[0008] Specifically, the present invention relates to the use of isoinulin lactone or its derivatives as pesticide synergists in aphid control, wherein the pesticides include thiamethoxam, sulfoxaflor, clothianidin, imidacloprid, pirimicarb, deltamethrin, cypermethrin, malathion, avermectin, and fenitrothion, and the concentration of isoinulin lactone or its derivatives is not less than 2 μg / ml, and the preferred concentration of isoinulin lactone or its derivatives is 2 μg / ml to 10 μg / ml, and the aphids include corn aphids, cereal aphids, pea aphids or cotton aphids, etc.

[0009] Preferably, the pesticide is 25% thiamethoxam, 48% clothianidin or 22% sulfoxaflor.

[0010] The preferred concentration of isoinulactone is 2 μg / mL and the pesticide is 25% thiamethoxam.

[0011] The preferred concentration of isoinulactone is 10 μg / mL and the pesticide is 25% thiamethoxam.

[0012] The preferred concentration of isoinulactone is 3 μg / mL and the pesticide is 48% clothianidin.

[0013] The preferred concentration of isoinulactone is 3 μg / mL and the pesticide is 22% sulfoxaflor.

[0014] The present invention identifies the biological activity of isoinulin lactone against aphids, and finds that even at a high concentration of 480 μg / ml, the mortality rates of isoinulin lactone against corn aphids, cereal aphids, pea aphids and cotton aphids are only 6.67±3.33%, 11.67±4.41%, 15.00±2.89% and 10.00±7.64%, respectively, indicating that isoinulin lactone itself has relatively low insecticidal activity against corn aphids, cereal aphids, pea aphids and cotton aphids. Indoor toxicity assays found that a mixture of 2 μg / mL isoinulactone and 25% thiamethoxam could significantly reduce the median lethal concentration (LC50) of thiamethoxam to corn aphids, cereal aphids, pea aphids and cotton aphids, with synergistic ratios of 3.382, 3.039, 4.097 and 2.266, respectively; a mixture of 0.4 μg / mL isoinulactone and 25% thiamethoxam could not enhance the insecticidal activity of thiamethoxam to corn aphids, cereal aphids and pea aphids; a mixture of 10 μg / mL isoinulactone and 25% thiamethoxam could significantly enhance the insecticidal activity of thiamethoxam to cereal aphids, with a synergistic ratio of 1.465, but the insecticidal activity to corn aphids and pea aphids was not significantly enhanced. Field efficacy tests showed that when 48% clothianidin 3.5 ml / mu (50% recommended dosage) was mixed with 3 μg / ml isoindolactone, the insect population reduction rate and control effect on corn aphids were increased by 7.44% and 9.76% respectively 7 days after application compared with 48% clothianidin 3.5 ml / mu alone; the insect population reduction rate and control effect on cereal aphids were increased by 17.00% (significant level) and 16.58% respectively compared with 48% clothianidin 3.5 ml / mu alone. 22% sulfoxaflor 5ml / mu (50% recommended dosage) was mixed with 3μg / ml isoinulin lactone. Seven days after the application, the insect population reduction rate and control effect of corn aphids were significantly increased by 6.74% and 8.88% respectively compared with the single 22% sulfoxaflor 5ml / mu; the insect population reduction rate and control effect of cereal aphids were significantly increased by 15.12% and 12.22% respectively compared with the single 22% sulfoxaflor 5ml / mu. Whether clothianidin or sulfoxaflor, the insect population reduction rate and control effect of corn aphids and cereal aphids after 50% recommended dosage and 3μg / ml isoinulin lactone were equivalent to 100% recommended dosage, indicating that 3μg / mL isoinulin lactone significantly improved the field control effect of the pesticides on aphids, while also reducing the actual field dosage of these pesticides. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the invention and, together with the description, serve to explain the principles of the invention.

[0016] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0017] Figure 1 It is the insecticidal effect of isoinulactone against corn aphids;

[0018] Figure 2 The insecticidal effect of isoinulactone on the cereal aphid;

[0019] Figure 3 It is the insecticidal effect of isoinulactone against pea aphids;

[0020] Figure 4 It is the insecticidal effect of isoinulactone against cotton aphids. DETAILED DESCRIPTION

[0021] The present invention is further described below by way of examples. It should be understood that the methods described in the examples of the present invention are only used to illustrate the present invention, rather than to limit the present invention, and simple improvements to the present invention under the premise of the concept of the present invention are within the scope of protection claimed by the present invention.

[0022] 1. Test reagents

[0023] Test insects: Corn aphids and cereal aphids were collected from corn fields in Shangbamiao Town, Enyang District, Bazhong, Sichuan in June 2024, and were continuously raised indoors with barley seedlings to maintain the population. Pea aphids and cotton aphids were both long-term indoor populations, and were continuously raised with pea seedlings and cotton seedlings, respectively, to maintain the population. The indoor breeding environment conditions were: temperature 26±1℃, relative humidity 50-60%, and photoperiod L:D=16:8.

[0024] Test reagents: isoinulin lactone (Shanghai Aladdin Biochemical Technology Co., Ltd., purity 98%), 25% thiamethoxam water dispersible granules (Shandong Bainongsida Biotechnology Co., Ltd.), anhydrous ethanol (analytical grade) and Tween-20 (Sinopharm Chemical Reagent Co., Ltd.), emulsifier 500# (Jiangsu Hai'an Petrochemical Plant), 48% clothianidin (Qingdao Haina Biotechnology Co., Ltd.), 22% sulfoxaflor (Dow AgroSciences (China) Co., Ltd.).

[0025] 2. Test methods

[0026] 2.1 Indoor toxicity test of isoindulenolide

[0027] Using the immersion method, the isoinulin lactone powder was first dissolved in anhydrous ethanol to prepare a 40 mg / ml stock solution, and then diluted with a 0.02% emulsifier (emulsifier 500#: Tween-20=1:2) aqueous solution to five concentration gradients of 480, 240, 120, 60 and 30 μg / ml, and a 0.02% emulsifier (emulsifier 500#: Tween-20=1:2) aqueous solution was used as a control. Select fresh hydroponic barley seedlings (about 4 cm in height, for corn aphids and cereal aphids) or pea seedlings (about 4 cm in height, for pea aphids) or fresh cotton leaves (a 3 cm diameter leaf disc cut by a hole puncher, for cotton aphids) with uniform growth, soak them in the above solution for 10 seconds, dry them in a cool place, place the dried seedlings or leaf discs (back side up) in a 55 cm diameter and 3 cm high plastic box (2% agar gel is added to the box and filter paper is covered to keep it moist), inoculate 20 wingless aphids of the same size in each hole, close the plastic box to prevent the aphids from escaping, and then place it in the aphid culture room. Set 3 independent biological replicates for each concentration, and evaluate the mortality rate after 24 hours.

[0028] Data analysis: SPSS22.0 software was used to perform the LSD (Least Significant Difference) method to compare and analyze the mortality rate under treatments with different concentrations of isoinulactone.

[0029] 2.2 Indoor toxicity test of isoinulactone and thiamethoxam mixture

[0030] A total of four treatments were set up for corn aphids: ① 25% thiamethoxam (2.5, 5, 10, 20, 30, 40μg / ml), ② 25% thiamethoxam (5, 10, 20, 30, 40μg / ml) + 0.4μg / ml isoinulactone, ③ 25% thiamethoxam (1, 2, 4, 8, 16μg / ml) + 2μg / ml isoinulactone, ④ 25% thiamethoxam (2.5, 5, 10, 15, 20, 25μg / ml) + 10μg / ml isoinulactone.

[0031] A total of four treatments were set up for the cereal aphid: ① 25% thiamethoxam (10, 20, 30, 40, 50, 60μg / ml), ② 25% thiamethoxam (10, 20, 30, 40, 50, 60μg / ml) + 0.4μg / ml isoinulactone, ③ 25% thiamethoxam (2.5, 5, 10, 15, 20, 25μg / ml) + 2μg / ml isoinulactone, ④ 25% thiamethoxam (5, 10, 20, 30, 40μg / ml) + 10μg / ml isoinulactone.

[0032] For pea aphid, four treatments were also set up: ① 25% thiamethoxam (5, 10, 20, 30, 40μg / ml), ② 25% thiamethoxam (5, 10, 20, 30, 40μg / ml) + 0.4μg / ml isoinulactone, ③ 25% thiamethoxam (1, 2, 4, 8, 16μg / ml) + 2μg / ml isoinulactone, ④ 25% thiamethoxam (2.5, 5, 10, 15, 20, 25μg / ml) + 10μg / ml isoinulactone.

[0033] For cotton aphid, two treatments were set up: ① 25% thiamethoxam (2.5, 5, 10, 20, 30, 40 μg / ml), ② 25% thiamethoxam (1, 2, 4, 8, 16 μg / ml) + 2 μg / ml isoindolactone.

[0034] The immersion method was also used, with 3-4 independent biological replicates for each concentration, and mortality was assessed after 24 hours.

[0035] Data analysis: The PROBIT model in SPSS Statistics 22.0 software was used to analyze the test data, and the toxicity regression equation, LC50 and 95% confidence interval of each treatment were calculated. Synergistic ratio = LC50 of insecticide against aphids / LC50 of insecticide (+ synergist) against aphids.

[0036] 2.3 Field efficacy test of isoinulactone mixed with pesticides against aphids

[0037] Experimental location: Baozang Town, Jiangcheng County, Yunnan Province.

[0038] Corn experimental variety: Xinda 901ZL (containing Cry1Ab+Vip3Aa+epsps)

[0039] Test date: September 13, 2024

[0040] Test agent: 48% clothianidin, 22% sulfoxaflor

[0041] Test method: Spray the artificial stems and leaves evenly, with a liquid volume of 40 liters per mu

[0042] Plot area: 100 square meters for each treatment (7 treatments in total, with three replicates for each treatment)

[0043] The experimental drug treatments were as follows:

[0044] (1) The recommended field dosage of 48% clothianidin is 7 ml / mu;

[0045] (2) 48% clothianidin dosage: 3.5 ml / mu;

[0046] (3) 48% clothianidin dosage 3.5 ml / mu + isoinulactone (final concentration 3 μg / ml);

[0047] (4) The recommended field dosage of 22% sulfoxaflor is 10 ml / mu;

[0048] (5) 22% sulfoxaflor dosage: 5 ml / mu;

[0049] (6) 22% sulfoxaflor nitrile dosage 5 ml / mu + isocarpin (final concentration 3 μg / ml);

[0050] (7) Clean water control.

[0051] First, dissolve the isoinulin lactone powder in anhydrous ethanol to prepare a 30mg / ml stock solution. When applied in the field, calculate according to the plot area and the amount of water per mu of spraying the agent to ensure that the final concentration of isoinulin lactone is 3μg / ml. Each treatment group and control group are set up with 3 plots (repeated), and 10 corn plants are surveyed in each plot (marked with a sign). During the survey, visually inspect the whole plant and record the number of aphids (corn aphids and cereal aphids) observed in the whole plant. Investigate the base number of aphids on the corn plants before applying the medicine, and investigate the amount of residual insects once 1, 3, and 7 days after applying the medicine. Compare with the base number before applying the medicine to calculate the insect population reduction rate and prevention effect. The formula for calculating the efficacy is as follows:

[0052] Pest reduction rate (%) = (number of insects before application - number of insects after application) / number of insects before application × 100%

[0053] Control effect (%) = (insect population reduction rate in the area treated with the pesticide - insect population reduction rate in the blank control area) / (1 - insect population reduction rate in the blank control area) × 100%.

[0054] 3. Test results

[0055] 3.1 Insecticidal activity of isoinulactone itself against aphids

[0056] The insecticidal effect of isoinulactone on four species of aphids Figure 1-Figure 4 The data in the figure are mean ± standard error. Different lowercase letters on the columns represent significant differences among different treatments (P < 0.05) (LSD least significant difference method).

[0057] The results of the indoor toxicity test of isoinulactone against corn aphids showed that even at the highest concentration of 480 μg / ml, the mortality rate was only 6.67±3.33%, which was not significantly different from the control group (1.67±1.67%) and the mortality rate at other concentrations (P>0.05) ( Figure 1). For the cereal aphid, the mortality rate at the highest concentration of 480μg / ml was 11.67±4.41%, which was not significantly different from the control group (5.00±2.89%) and the mortality rates at other concentrations (P>0.05) ( Figure 2 ). For pea aphids, the mortality rates of isoalelopathic acid from low to high concentrations (0-480 μg / ml) were 3.33±1.67%, 1.67±1.67%, 5.00±2.89%, 3.33±3.33%, 6.67±4.41% and 15.00±2.89%, respectively. The mortality rate at the highest concentration of 480 μg / ml was significantly higher than that at 30, 60 and 120 μg / ml and the control group (P<0.05), but there was no significant difference between the mortality rate and that at 240 μg / ml (P=0.070)( Figure 3 ). For cotton aphid, the mortality rate at the highest concentration of 480μg / ml was 10.00±7.64%, which was not significantly different from the control group (0.00±0.00%) and the mortality rates at other concentrations (P>0.05) ( Figure 4 ). These results suggest that isoinulactone itself has low insecticidal activity against corn aphid, cereal aphid, pea aphid, and cotton aphid.

[0058] 3.2 Indoor synergistic effect of isoinulactone on thiamethoxam

[0059] 3.2.1 Effect of a mixture of isoinulactone and thiamethoxam on corn aphids

[0060] Table 1 Insecticidal activity of the mixture of isoinulactone and thiamethoxam against corn aphids

[0061]

[0062] The results of indoor bioassay showed that the median lethal concentration (LC50 value) of 25% thiamethoxam water dispersible granules to corn aphids was 14.267 (11.605-17.632) μg / ml. When 0.4 μg / ml, 2 μg / ml and 10 μg / ml of isoinulactone were added, the LC50 values ​​were 15.373 (12.866-18.120), 4.218 (3.456-5. 104) and 10.225 (7.045-14.407) μg / ml, with synergistic ratios of 0.928, 3.382 and 1.395, respectively. The LC50 value of adding 2 μg / ml isoinulactone was significantly lower than the LC50 value without adding isoinulactone, and the 95% confidence intervals of the two did not overlap, indicating that the insecticidal activity of thiamethoxam against corn aphids can be significantly improved by mixing with 2 μg / ml isoinulactone (Table 1).

[0063] 3.2.2 Effect of a mixture of isoinulactone and thiamethoxam on Psoralea graminearum

[0064] Table 2 Insecticidal activity of the mixture of isoinulactone and thiamethoxam against Rhizopus graminearum

[0065]

[0066] It can be found from Table 2 that the lethal median concentration (LC50 value) of 25% thiamethoxam water dispersible granules to cereal aphids is 32.230 (27.448-37.644). When mixed with 0.4 μg / ml, 2 μg / ml and 10 μg / ml isoinulactone, the LC50 values ​​are 28.514 (24.747-32.203), 10.604 (8.314-13.464) and 21.998 (18.312-26.663) μg / ml, respectively. ml, the synergistic ratios were 1.130, 3.039 and 1.465 respectively, among which the LC50 values ​​of adding 2μg / ml and 10μg / ml isoinulactone were significantly lower than the LC50 value without adding isoinulactone, and the 95% confidence intervals of the three did not overlap, indicating that the insecticidal activity of thiamethoxam against cereal aphids can be significantly improved by mixing with 2μg / ml and 10μg / ml isoinulactone, and the enhancement effect of 2μg / ml is better than that of 10μg / ml (Table 2).

[0067] 3.2.3 Effect of a mixture of isoinulactone and thiamethoxam on pea aphids

[0068] The results of bioassay showed that the median lethal concentration (LC50 value) of 25% thiamethoxam water dispersible granules to pea aphids was 23.167 (20.482-26.158) μg / ml. When 0.4, 2 and 10 μg / ml of isoinulactone were added, the LC50 values ​​were 24.250 (20.159-30.163), 5.655 (4.734-6.803) and 19. 585 (16.396~25.060) μg / ml, and the synergistic ratios were 0.955, 4.097 and 1.183, respectively. The LC50 value of adding 2 μg / ml isoinulactone was significantly lower than the LC50 value without adding isoinulactone, and there was no overlapping area in the 95% confidence intervals of the two, indicating that the insecticidal activity of thiamethoxam against pea aphids can be significantly improved by mixing with 2 μg / ml isoinulactone (Table 3).

[0069] Table 3 Insecticidal activity of the mixture of isoinulactone and thiamethoxam against pea aphids

[0070]

[0071] 3.2.4 Effect of a mixture of isoinulactone and thiamethoxam on cotton aphids

[0072] Table 4 Insecticidal activity of the mixture of isoinulactone and thiamethoxam against cotton aphids

[0073]

[0074] The median lethal concentration (LC) of 25% thiamethoxam water dispersible granules to cotton aphids 50 value) is 13.425

[0075] (8.429~20.053), when mixed with 2μg / ml isoindulenolide, its LC 50 The value dropped to 5.924 (4.627-7.851) μg / ml, and the 95% confidence intervals of the two did not overlap, indicating that the addition of 2 μg / ml

[0076] Isoinulin can significantly enhance the insecticidal activity of thiamethoxam against cotton aphids, with a synergistic ratio of 2.266

[0077] (Table 4).

[0078] 3.3 Field synergistic effect of isoinulactone on clothianidin and sulfoxaflor

[0079] 3.3.1 Effect of a mixture of isoinulactone and clothianidin on aphid control

[0080] The field efficacy results of the mixture of clothianidin and isoinulin lactone on corn aphids showed that 4 days and 7 days after application, the insect population reduction rate and control effect of treatment 1 (recommended dosage of clothianidin 7 ml / mu) were significantly higher than those of treatment 2 (3.5 ml / mu of clothianidin). 4 days after application, the insect population reduction rate and control effect of treatment 3 (3.5 ml / mu of clothianidin + 3ug / ml isoinulin lactone) increased by 9.94% and 6.44% respectively compared with treatment 2; 7 days after application, the insect population reduction rate and control effect increased by 7.44% and 9.76% respectively, indicating that 3ug / ml isoinulin lactone can improve the field control effect of clothianidin on corn aphids (Table 5).

[0081] Table 5 Field efficacy of a mixture of clothianidin and isoinulactone against corn aphids

[0082]

[0083] The field efficacy results of the mixture of clothianidin and isoinulin against the aphid showed that 7 days after the application, the insect population reduction rates of treatment 1 (recommended dosage of clothianidin 7 ml / mu) and treatment 3 (3.5 ml / mu of clothianidin + 3 ug / ml isoinulin) were significantly higher than those of treatment 2 (3.5 ml / mu of clothianidin), and there was no significant difference between treatment 1 and treatment 3; the insect population reduction rate of treatment 3 was significantly increased by 17.00% compared with treatment 2. 7 days after the application, although the control effects of the three treatments were not statistically significant, treatment 3 was also increased by 16.58% compared with treatment 2. These results show that 3 ug / ml isoinulin can significantly improve the field control effect of clothianidin against the aphid, thereby reducing the actual field dosage of clothianidin (Table 6).

[0084] Table 6 Field efficacy of the mixture of clothianidin and isoinulactone against Psoralea graminicola

[0085]

[0086] 3.3.2 Effect of a mixture of isoinulactone and sulfoxaflor on aphid control

[0087] The field efficacy results of the mixture of sulfoxaflor and isoinulactone on corn aphids showed that 7 days after application, the insect population reduction rate and control effect of treatment 1 (recommended dosage of sulfoxaflor 10 ml / mu) and treatment 3 (sulfoxaflor 5 ml / mu + isoinulactone 3ug / ml) were significantly higher than those of treatment 2 (sulfoxaflor 5 ml / mu), and there were no significant differences in the insect population reduction rate and control effect between treatment 1 and treatment 3; the insect population reduction rate and control effect of treatment 3 were significantly increased by 6.74% and 8.88% compared with treatment 2, respectively; this shows that 3ug / ml isoinulactone can significantly improve the field control effect of sulfoxaflor on corn aphids, thereby reducing the actual field dosage of sulfoxaflor (Table 7).

[0088] Table 7 Field efficacy of sulfoxaflor and isoinulactone mixed with corn aphid

[0089]

[0090] Table 8 Field efficacy of the mixture of sulfoxaflor and isoinulactone against Pseudomonas australis

[0091]

[0092] The field efficacy results of the mixture of sulfoxaflor and isoinulactone on the aphid of cereals and grains showed that: 7 days after application, the insect population reduction rate and control effect of treatment 1 (recommended dosage of sulfoxaflor 10 ml / mu) and treatment 3 (sulfoxaflor 5 ml / mu + isoinulactone 3ug / ml) were significantly higher than those of treatment 2 (sulfoxaflor 5 ml / mu), and there were no significant differences in the insect population reduction rate and control effect between treatment 1 and treatment 3; the insect population reduction rate and control effect of treatment 3 were significantly increased by 15.12% and 12.22% respectively compared with treatment 2; this shows that 3ug / ml isoinulactone can significantly improve the field control effect of sulfoxaflor on the aphid of cereals and grains, thereby reducing the actual field dosage of sulfoxaflor (Table 8).

[0093] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. Application of isoinulin lactone or its derivatives as pesticide synergists in aphid control.

2. The use of isoinulin lactone or its derivatives as pesticide synergist in aphid control according to claim 1, characterized in that: The pesticides include thiamethoxam, sulfoxaflor, clothianidin, imidacloprid, pirimicarb, deltamethrin, cypermethrin, marathon, avermectin, and fenitrothion.

3. Use of isoinulin lactone or its derivatives as pesticide synergist in aphid control according to claim 1, characterized in that: The concentration of isoinulin lactone or its derivative is not less than 2 μg / ml.

4. Use of isoinulin lactone or its derivatives as pesticide synergist in aphid control according to claim 3, characterized in that: The aphids include corn aphid, cereal aphid, pea aphid or cotton aphid.

5. Use of isoinulin lactone or its derivatives as pesticide synergist in aphid control according to claim 3, wherein the concentration of isoinulin lactone or its derivatives is 2 μg / ml to 10 μg / ml.

6. Use of isoinulin lactone or its derivatives as pesticide synergist in aphid control according to claim 2, characterized in that: The pesticide is 25% thiamethoxam, 48% clothianidin or 22% sulfoxaflor.

7. Use of isoinulin lactone or its derivatives as pesticide synergist in aphid control according to claim 2, characterized in that: The concentration of isoinulactone is 2 μg / mL, and the pesticide is 25% thiamethoxam.

8. Use of isoinulin lactone or its derivatives as pesticide synergist in aphid control according to claim 2, characterized in that: The concentration of isoinulactone is 10 μg / mL, and the pesticide is 25% thiamethoxam.

9. Use of isoinulin lactone or its derivatives as pesticide synergist in aphid control according to claim 2, characterized in that: The concentration of isoinulactone is 3 μg / mL, and the pesticide is 48% clothianidin.

10. Use of isoinulin lactone or its derivatives as pesticide synergist in aphid control according to claim 2, characterized in that: The concentration of isoinulactone is 3 μg / mL, and the pesticide is 22% sulfoxaflor.

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