Seed germination method of immature citron fruits
Through the method of collecting, processing and spraying water-soluble fertilizers of immature citron fruit seeds, the problem of lack of seed germination methods for immature fruit seeds in the prior art is solved, and efficient germination of citron seeds and expansion of seed sources are achieved.
Patent Information
- Application Number
- CN202510114094.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-24
- Publication Date
- 2025-03-07
AI Technical Summary
The prior art mainly uses seeds of mature fruits for citrus seed germination, and lacks methods for seed germination of immature fruits, which limits the source of test materials for citron seed reproduction and seed selection.
Use the seeds of immature citron fruits, collect, peel, clean and remove the outer seed coat, and spray Huaduoduo No. 1 water-soluble fertilizer on the moist filter paper until the seeds germinate.
The efficient germination of immature citron seeds has been achieved, and the source of seeds has been expanded for the reproduction and selection of citron seeds, providing more test materials.
Smart Images
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of plant propagation, and in particular relates to a seed germination method for immature citron fruits. Background Art
[0002] citron( Citrus medica L.) is a fruit tree of the genus Citrus in the Rutaceae family, which has rich nutritional and medicinal value. Citron seeds are monoembryonic seeds, which develop into sexual embryos through pollination and fertilization. After sowing, each individual plant is a hybrid with rich variation types. Therefore, sowing with citron seeds can be used for seed selection, and the seeds are generally virus-free.
[0003] At present, there are many studies on citrus seed germination, and usually the seeds of mature fruits are used. The present invention uses the seeds of immature citron fruits as materials to establish a citron seed germination method, which can expand the source of seeds for citron seed propagation and seed selection. Summary of the invention
[0004] The purpose of the present invention is to provide a method for germinating seeds of immature citron fruits, so as to provide more test materials for carrying out research on seed propagation and seed selection.
[0005] To achieve the above object, the present invention adopts the following technical solution: A method for germinating seeds of immature citron fruits, comprising the following steps: (1) Collecting immature citron fruits; (2) Peel the fruit, take out the seeds, wash off the attachments on the surface of the seeds with clean water, and then remove the outer seed coat; (3) Spread the seeds with the outer seed coat removed on three layers of moist filter paper and cover them with two layers of moist filter paper. Spray them with Huaduoduo No. 1 water-soluble fertilizer once every 7 days until the seeds germinate.
[0006] Furthermore, in step (1), citron fruits with yellow-green peel are collected in late September.
[0007] Furthermore, in step (2), the method for removing the exocarp is to spread the seeds on moist filter paper for 2 to 3 days and then remove the exocarp after it softens.
[0008] Furthermore, in step (3), the seeds spread on the wet filter paper should be spaced at least 0.1 cm apart.
[0009] Furthermore, the Huaduoduo No. 1 water-soluble fertilizer in step (3) is specifically 2000~3000 times diluted.
[0010] The significant advantages of the present invention are: The present invention establishes a seed germination method for immature citron fruits for the first time, and realizes efficient germination of immature citron seeds, thereby expanding the source of seeds for citron seed propagation and seed selection. BRIEF DESCRIPTION OF THE DRAWINGS
[0011] Figure 1 Sample photos of unripe citron fruits (left) and ripe citron fruits (right).
[0012] Figure 2 Sample pictures of citron seeds without the outer seed coat removed (left) and citron seeds with the outer seed coat removed (right).
[0013] Figure 3 This is a sample photo of germination of immature citron fruit seeds in Example 1 (the arrow in the figure indicates a 0.5 cm radicle). DETAILED DESCRIPTION
[0014] In order to make the contents of the present invention easier to understand, the technical solution of the present invention is further described below in conjunction with specific implementation methods, but the present invention is not limited thereto. Example
[0015] A method for germinating seeds of immature citron fruits, comprising the following steps: (1) In late September, collect immature citron fruits with yellow-green skin; (2) Peel the fruit, take out the seeds, wash the attachments on the surface of the seeds with clean water, and then spread the seeds flat on moist filter paper for 2 days. After the outer seed coat softens, remove the outer seed coat; (3) Spread the seeds with the outer seed coat removed on three layers of moist filter paper. There should be at least 0.1 cm between seeds and cover them with two layers of moist filter paper. Spray with 2000 times diluted Huaduoduo No. 1 water-soluble fertilizer every 7 days until the seeds germinate.
[0016] Comparative Example 1 (1) In late October, collect ripe citron fruits with yellow skin; (2) Peel the fruit, take out the seeds, wash the attachments on the surface of the seeds with clean water, and then spread the seeds flat on moist filter paper for 2 days. After the outer seed coat softens, remove the outer seed coat; (3) Spread the seeds with the outer seed coat removed on three layers of moist filter paper. There should be at least 0.1 cm between seeds and cover them with two layers of moist filter paper. Spray with 2000 times diluted Huaduoduo No. 1 water-soluble fertilizer every 7 days until the seeds germinate.
[0017] Table 1 Comparison of germination effect between immature fruit and mature fruit
[0018] As shown in Table 1, there is no significant difference in the effect of seed germination using immature fruits and mature fruits as materials. Therefore, using seeds from immature citron fruits can provide more seed sources for citron seed propagation and seed selection.
[0019] Comparative Example 2 (1) In late September, collect immature citron fruits with yellow-green skin; (2) Peel the fruit, take out the seeds, and wash off any attachments on the surface of the seeds with clean water; (3) Spread the washed seeds flat on three layers of moist filter paper, with at least 0.1 cm between seeds. Cover them with two layers of moist filter paper and spray with 2000 times diluted Huaduoduo No. 1 water-soluble fertilizer every 7 days until the seeds germinate.
[0020] Table 2 Comparison of germination effects after removal of the outer seed coat
[0021] As shown in Table 2, removing the outer seed coat can effectively shorten the germination days and improve the germination potential, germination potential and germination speed index.
[0022] Comparative Example 3 (1) In late September, collect immature citron fruits with yellow-green skin; (2) Peel the fruit, take out the seeds, wash the attachments on the surface of the seeds with clean water, and then spread the seeds flat on moist filter paper for 2 days. After the outer seed coat softens, remove the outer seed coat; (3) Spread the seeds with the outer seed coat removed on three layers of moist filter paper. There should be at least 0.1 cm between seeds and cover them with two layers of moist filter paper. Spray different water-soluble fertilizers every 7 days until the seeds germinate.
[0023] Table 3 Comparison of germination effects of different water-soluble fertilizers
[0024] It can be seen from Table 3 that compared with the application of clear water and Stanley, spraying 2000~3000 times diluted Huaduoduo No. 1 water-soluble fertilizer can shorten the germination days and improve the germination potential, germination potential and germination speed index.
[0025] The above description is only a preferred embodiment of the present invention. All equivalent changes and modifications made according to the scope of the patent application of the present invention should fall within the scope of the present invention.
Claims
1. A method for germinating seeds of immature citron fruits, characterized in that: The following steps are involved: (1) Collecting immature citron fruits; (2) Peel the fruit, take out the seeds, wash off the attachments on the surface of the seeds with clean water, and then remove the outer seed coat; (3) Spread the seeds with the outer seed coat removed on three layers of moist filter paper and cover them with two layers of moist filter paper. Spray them with Huaduoduo No. 1 water-soluble fertilizer once every 7 days until the seeds germinate.
2. The method for germinating seeds of immature citron fruits according to claim 1, characterized in that: In step (1), specifically, citron fruits with yellow-green peel are collected in late September.
3. The method for germinating seeds of immature citron fruits according to claim 1, characterized in that: The method for removing the exocarp in step (2) is to spread the seeds flat on moist filter paper for 2 to 3 days and then remove the exocarp after it softens.
4. The method for germinating seeds of immature citron fruits according to claim 1, characterized in that: In step (3), the seeds spread on the moistened filter paper should be spaced at least 0.1 cm apart.
5. The method for germinating seeds of immature citron fruits according to claim 1, characterized in that: The Huaduoduo No. 1 water-soluble fertilizer described in step (3) is specifically 2000~3000 times diluted.