Turmeric meal fermented feed as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of fermentation engineering, and particularly relates to turmeric meal fermented feed as well as a preparation method and application thereof. Iron, magnesium and zinc elements are added into fermentation base materials such as turmeric meal and soybean meal, and the mixture is fermented through a bacterium-enzyme synergistic fermentation inoculant to generate the turmeric meal fermented feed, wherein the fermentation inoculant comprises bacillus subtilis, lactobacillus plantarum and saccharomycetes. The prepared turmeric meal fermented feed can be applied to feed feeding of laying hens or broilers. Results of the embodiment of the invention show that the turmeric meal fermented feed prepared by the invention not only can effectively inhibit harmful bacteria in vitro, but also can remarkably improve the chicken production performance, the eggshell quality and the yolk color, and effectively improve the chicken quality of yellow feather broilers.

Description

Technical Field

[0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a turmeric meal fermented feed and a preparation method and application thereof. Background Art

[0002] Turmeric is a common Chinese medicinal material used for both medicine and food. It is the dried rhizome of the perennial herb of the genus Curcuma in the ginger family. Its main active ingredient is curcumin. The Chinese Pharmacopoeia records that turmeric has the effects of breaking blood and promoting qi, relieving pain through menstruation, and clearing the heart and relieving depression. Modern medical research has shown that turmeric has multiple biological functions such as antioxidant, anti-tumor, regulating fat metabolism and enhancing immunity. It can improve the disease resistance, immune system function and growth performance of livestock and poultry, and has a wide range of preventive effects on diseases. Turmeric meal is the product of turmeric after curcumin is extracted through technological technology. Although the pharmacological effect is relatively weakened, it has a low price as a feed raw material and has more application advantages.

[0003] Curcumin and its derivatives have antibacterial effects on Streptococcus pneumoniae, Staphylococcus aureus, Escherichia coli, Helicobacter pylori, Salmonella, etc. Curcumin can inhibit the generation of free radicals in animals, remove the generated free radicals, enhance the activity of various antioxidant enzymes in the body, enhance the body's antioxidant capacity, and reduce oxidative stress. Turmeric meal is a by-product of extracting curcumin from turmeric. It can be used as a feed-grade raw material. It has a low crude protein content and a crude fiber content of about 15%. However, the turmeric meal raw material has a pungent odor, which affects the feed intake. It is rarely added directly to livestock and poultry feed. In the prior art, turmeric meal is mostly directly added to livestock and poultry feed, and the amount used is only 1%-2%.

[0004] Therefore, it is urgent to develop a feed produced by utilizing turmeric meal. Summary of the invention

[0005] The purpose of the present invention is to provide a turmeric meal fermented feed. The fermented feed provided by the present invention can not only reduce the fiber content in the feed through the synergistic fermentation of bacteria and enzymes, but also significantly increase the amount of the feed directly added to livestock and poultry feed with a mellow smell.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] The invention provides a turmeric meal fermented feed, which is characterized in that the turmeric meal fermented feed is fermented by fermentation base material through fermentation bacteria agent; the fermentation base material is a crushed material of 30-80% by weight of turmeric meal, 5-20% by weight of soybean meal, 5-20% by weight of corn and 10-30% by weight of bran; the fermentation bacteria agent comprises bacillus subtilis, lactobacillus plantarum and yeast.

[0008] Preferably, the Bacillus subtilis is deposited in the China General Microbiological Culture Collection Center (CGMCC) with a deposit number of CGMCC NO.31665; the Lactobacillus plantarum is deposited in the China Industrial Microbiological Culture Collection Center with a deposit number of CICC 24936.

[0009] The present invention also provides a method for preparing the fermented feed, comprising adding ferrous sulfate, zinc sulfate, manganese sulfate, complex enzyme and purified water to the fermentation base material, inoculating a fermentation agent, mixing and fermenting to generate the fermented feed;

[0010] The complex enzyme comprises alkaline protease, xylanase and cellulase.

[0011] Preferably, the total amount of ferrous sulfate, zinc sulfate and manganese sulfate added is 0.4% of the weight of the fermentation base material, and the addition ratio of ferrous sulfate, zinc sulfate and manganese sulfate is 2:3:3.

[0012] Preferably, the addition amount of the complex enzyme is 0.2% of the weight of the fermentation base; the enzyme activity content ratio of protease, cellulose and xylanase in the complex enzyme is 1:1:1; the alkaline protease is 100,000 U / g alkaline protease, the xylanase is 100,000 U / g xylanase, and the cellulase is 8000 U / g cellulase; the weight ratio of the pure water to the fermentation base is 10:(4-5).

[0013] Preferably, the total inoculation amount of Bacillus subtilis and plant lactic acid bacteria in the fermentation agent is 0.4%-1%, and the inoculation ratio is 1:1; the inoculation amount of yeast is calculated as active dry yeast, and the inoculation amount is 5×10 6 CFU / g.

[0014] Preferably, the fermentation culture temperature is 35-37°C, the culture period is 3-7 days, and the effective viable bacteria count of the fermented feed is 5.0×10 8 -1.0×10 10 cfu / g.

[0015] The present invention also provides the use of the turmeric meal fermented feed or the fermented feed prepared by the preparation method in feeding laying hens or broilers; the fermented feed can be fed directly or made into granules or powder for feeding, and the amount of the turmeric meal fermented feed added in the feeding is 2%-10%.

[0016] The present invention also provides application of the turmeric meal fermented feed or the fermented feed prepared by the preparation method in improving the production performance of laying hens, eggshell quality or yolk color.

[0017] The present invention also provides the use of the turmeric meal fermented feed or the fermented feed prepared by the above preparation method in improving the meat quality of yellow-feathered broilers.

[0018] Beneficial effects of the present invention:

[0019] 1. The turmeric meal in the turmeric meal fermented feed of the present invention can not only reduce the fiber content in the feed through the synergistic fermentation of bacteria and enzymes, but also has a mellow and non-pungent smell, and at the same time enhances the inhibitory effect on harmful bacteria such as Clostridium perfringens and Escherichia coli.

[0020] 2. The turmeric meal fermented feed prepared by the present invention is applied to feed for laying and broiler poultry, which can improve the egg production rate of laying hens, reduce the rate of soft and broken eggs, increase egg weight and yolk color, improve the quality of yellow-feathered broilers, and reduce the mortality rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0022] Figure 1 The results of in vitro antibacterial tests of turmeric meal fermented feed prepared in Comparative Examples 1-3 and Example 2 (indicator bacteria: Clostridium perfringens), the first row from left to right are Comparative Example 1 and Comparative Example 2, and the second row from left to right are Comparative Example 3 and Example 2;

[0023] Figure 2 The results of in vitro antibacterial tests of turmeric meal fermented feed prepared in Comparative Examples 1-3 and Example 2 (indicator bacteria: Escherichia coli) are shown in the first row from left to right: Comparative Example 1 and Comparative Example 2, and the second row from left to right: Comparative Example 3 and Example 2. DETAILED DESCRIPTION

[0024] The invention provides a turmeric meal fermented feed, which is prepared by fermenting turmeric meal as a fermentation raw material through bacterial enzymes and a synergistic fermentation agent.

[0025] The invention prepares 30-80% of turmeric meal, 5-20% of soybean meal, 5-20% of corn and 10-30% of bran, preferably 60% of turmeric meal, 7% of soybean meal, 8% of corn and 25% of bran, mixes and grinds them into a fineness of 2.00 mm as a fermentation base material, adds ferrous sulfate, manganese sulfate and zinc sulfate, and inoculates a complex enzyme and a fermentation bacterial agent to obtain turmeric meal fermented feed through fermentation.

[0026] The biological deposit information of Bacillus subtilis 1 is: deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, the deposit time is: August 19, 2024, the address is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the unit abbreviation is: CGMCC, the biological deposit number is CGMCC No.31665, and the classification name is: Bacillus subtilis.

[0027] Adding ferrous sulfate to fermented feed can supplement iron and promote the synthesis of hemoglobin in animals, thereby improving the health of animals. Adding manganese sulfate to feed can supplement manganese and promote the health and growth of animals. Adding zinc sulfate to feed can supplement zinc and promote the health and growth of animals. Zinc assists in the synthesis of biological enzymes, maintains the integrity of cell membranes, participates in the synthesis of sex hormones in the body, and promotes the metabolism of vitamin A. The total amount of ferrous sulfate, zinc sulfate and manganese sulfate added is 0.4% of the weight of the fermentation base, and the addition ratio of ferrous sulfate, zinc sulfate and manganese sulfate is 2:3:3; the content of iron, zinc and manganese in ferrous sulfate, manganese sulfate and zinc sulfate is greater than 30%.

[0028] In the bacterial-enzyme synergistic fermentation, the amount of compound enzyme added is 0.2% of the weight of the fermentation base material. The compound enzyme used includes 100,000 U / g alkaline protease, 100,000 U / g xylanase and 8000 U / g cellulase, and the enzyme activity content ratio of the three added amounts is 1:1:1. The fermentation bacteria agent includes Bacillus subtilis, plant lactic acid bacteria and yeast. The total inoculation amount of Bacillus subtilis agent and plant lactic acid bacteria agent is 0.4%-1%, preferably 0.6%, and the inoculation ratio is 1:1. The yeast inoculation amount is calculated as active dry yeast, and the inoculation amount is 5×10 6 CFU / g. The weight ratio of pure water to fermentation base is 10:(4-5), preferably 5:2. The fermentation temperature is 35-37°C, and the culture is 3-7 days, preferably 37°C, and the culture is 3 days; the effective viable bacteria count of the fermented feed is 5.0×10 8 -1.0×10 10 cfu / g

[0029] The fermented turmeric meal feed prepared by the present invention and the comparative example were subjected to an in vitro antibacterial test for comparison. The results of the example show that the fermented turmeric meal feed prepared by the present invention can significantly inhibit the growth of harmful bacteria.

[0030] The turmeric meal fermented feed prepared by the present invention can be applied to egg-laying poultry and broiler feed. The results of the embodiments of the present invention show that the turmeric meal fermented feed prepared by the present invention can improve the egg production rate of laying hens, reduce the rate of soft and broken eggs, increase egg weight and yolk color, improve the quality of yellow-feathered broiler meat, and reduce the mortality rate.

[0031] The turmeric meal fermented feed of the present application decomposes or transforms macromolecular substances and anti-nutritional factors in the feed through the metabolism and bacterial reproduction of microorganisms. In this process, a variety of organic acids, biological enzymes, vitamins, amino acids and other unknown growth factors will be produced, which are more conducive to animal feeding and utilization. The turmeric meal raw material is subjected to microbial fermentation treatment, the crude fiber is degraded to a certain extent, the neutral detergent fiber is reduced, and the palatability is enhanced. At the same time, the cell wall structure of the turmeric meal is destroyed by microorganisms, and the residual curcumin is released to the maximum extent, and the antioxidant and antibacterial properties are enhanced. The pungent smell of the turmeric meal raw material is improved through microbial fermentation, and the amount of turmeric meal in livestock and poultry feed is increased, which reduces costs.

[0032] In order to further illustrate the present invention, the technical solution provided by the present invention is described in detail below in conjunction with the accompanying drawings and embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0033] Bacillus subtilis 1 of the present invention is deposited in the General Microbiological Center of China Microorganism Culture Collection Committee (CGMCC) with a deposit number of CGMCCNO.31665 and a classification name of Bacillus subtilis; Lactobacillus plantarum was purchased from China Industrial Microorganism Culture Collection Center with a number of CICC 24936. Yeast was purchased from Angel Yeast Co., Ltd. with a product number of 82000657; Bacillus subtilis 2 of the present invention was purchased from Guangdong Microorganism Culture Collection Center (GDMCC) with a number of 1.3819.

[0034] The basic daily diet for laying hens was purchased from Tangrenshen Co., Ltd.'s Jiale 623 laying hen feed; the basic daily diet for broilers was purchased from Tangrenshen Co., Ltd.'s Jiale 613 broiler complete feed.

[0035] The production processes, experimental methods or detection methods involved in the embodiments of the present invention, unless otherwise specified, are all conventional methods in the prior art, and their names and / or abbreviations are all conventional names in the field, and are very clear and unambiguous in the relevant application fields. Technical personnel in the field can understand the conventional process steps based on the names and apply the corresponding equipment, and implement them according to conventional conditions or the conditions recommended by the manufacturer.

[0036] The various instruments, equipment, raw materials or reagents used in the embodiments of the present invention are not particularly limited in terms of their sources, and are all conventional products that can be purchased through regular commercial channels, or can be prepared according to conventional methods well known to those skilled in the art.

[0037] Example 1 Preparation of fermentation agent

[0038] 1. Preparation of Bacillus subtilis:

[0039] S1. Preparation of seed solution: Pick activated Bacillus subtilis colonies and inoculate them into sterile LB medium. The culture conditions are 37°C, aerobic, constant temperature shaking incubator for 10h, speed 200r / min, to prepare seed solution;

[0040] S2. Liquid fermentation: LB culture medium and organosilicon defoamer are added to the fermenter for sterilization, and the mass ratio of organosilicon defoamer to culture medium is 100:0.05. The seed liquid prepared in step S1 is inoculated into a sterilized LB culture medium fermenter at an inoculum amount of 3% (volume ratio of seed liquid to culture medium) for culture, and the fermentation conditions are: tank pressure 0.05Mpa, air flow 15L / min, rotation speed 225r / min, temperature 37°C, fermentation culture for 48h, and obtain Bacillus subtilis 1.

[0041] 2. Preparation of plant lactobacillus agent:

[0042] S1. Seed solution preparation: Take the purified Lactobacillus plantarum slant and inoculate it into sterilized MRS broth medium, culture it at 37°C, anaerobic, constant temperature for 12h to prepare seed solution;

[0043] S2. Liquid fermentation: Add MRS liquid culture medium and organosilicon defoamer to the fermenter for sterilization, and the mass ratio of organosilicon defoamer to culture medium is 100:0.05. The seed liquid cultured in step S1 is inoculated into the fermenter at a volume ratio of 1.0% of the seed liquid to the culture medium and cultured for 48 hours until the pH is lower than 4.0. The fermentation conditions are: tank pressure 0.01Mpa, ventilation volume 0L / min, rotation speed 0r / min, temperature 37°C, and obtain plant lactobacillus.

[0044] The comparative example Bacillus subtilis 2 was purchased from Guangdong Microbial Culture Collection Center (GDMCC) with the serial number 1.3819. The preparation process of Bacillus subtilis 2 and Lactobacillus plantarum used in the comparative example was the same as above.

[0045] Example 2 Preparation of turmeric meal fermented feed

[0046] S1. Mix 60% by weight of turmeric meal, 7% by weight of soybean meal, 8% by weight of corn and 25% by weight of bran, and grind them into a fermentation base material with a fineness of 2.0 mm.

[0047] S2. Add 0.1% ferrous sulfate, 0.15% zinc sulfate and manganese sulfate, and 0.2% complex enzyme to the fermentation base material, then add purified water at a ratio of 5:2 to the fermentation base material, and inoculate the fermentation bacteria Bacillus subtilis 1, implant lactobacillus and yeast.

[0048] S3, mixing and stirring the above mixture evenly, placing it at a temperature of 37° C., and fermenting it for 3 days to obtain turmeric meal fermented feed.

[0049] Comparative Example 1 Preparation of fermented feed

[0050] S1. 17% by weight of soybean meal, 18% by weight of corn and 65% by weight of bran are mixed evenly, and then crushed into a fermentation base material with a fineness of 2.0 mm.

[0051] S2. Add 0.1% ferrous sulfate, 0.15% zinc sulfate and manganese sulfate, and 0.2% complex enzyme to the fermentation base, then add purified water at a ratio of 5:2 to the fermentation base liquid, and inoculate fermentation bacteria Bacillus subtilis 1, Lactobacillus plantarum and yeast.

[0052] S3, mixing and stirring the above mixture evenly, placing it at a temperature of 37° C., and fermenting it for 3 days to obtain turmeric meal fermented feed.

[0053] Comparative Example 2 Preparation of turmeric meal fermented feed

[0054] S1. Mix 60% by weight of turmeric meal, 7% by weight of soybean meal, 8% by weight of corn and 25% by weight of bran, and grind them into a fermentation base material with a fineness of 2.0 mm.

[0055] S2, adding 0.1% ferrous sulfate, 0.15% zinc sulfate and manganese sulfate, 0.2% complex enzyme to the fermentation base material, and then adding purified water with a ratio of 5:2 to the fermentation base material liquid, and inoculating fermentation bacteria Bacillus subtilis 2, plant lactobacillus and yeast. The plant lactobacillus and yeast used are the same as those used in Example 2.

[0056] S3, mixing and stirring the above mixture evenly, placing it at a temperature of 37° C., and fermenting it for 3 days to obtain turmeric meal fermented feed.

[0057] Comparative Example 3 Preparation of Unfermented Turmeric Meal

[0058] S1. Mix 60% by weight of turmeric meal, 7% by weight of soybean meal, 8% by weight of corn and 25% by weight of bran, and grind them into a fermentation base material with a fineness of 2.0 mm.

[0059] S2. Add 0.1% ferrous sulfate, 0.15% zinc sulfate and manganese sulfate, and 0.2% complex enzyme to the fermentation base material, and then add pure water in a ratio of 5:2 to the fermentation base material, and mix well.

[0060] Example 3 In vitro antibacterial test

[0061] The feed utilization indicator bacteria: Clostridium perfringens and Escherichia coli prepared in Example 2 of the present invention and Comparative Examples 1-3 were subjected to an in vitro antibacterial test.

[0062] The antibacterial effect was determined using the Oxford cup in vitro antibacterial test method.

[0063] In this antibacterial test, two parallel groups were set for each sample to be tested, and 150 μl of the sample treatment solution to be tested was added to each well. The indicator bacteria were Clostridium perfringens and Escherichia coli (concentration of 1×10 6 cfu / mL), cultured at 37℃, the experiment was terminated when the inhibition zone was clearly visible, the culture period was generally 7-9h. Use a vernier caliper to read the diameter of the inhibition zone and record the test results.

[0064] The results of the in vitro test with Clostridium perfringens as the indicator bacteria are as follows Figure 1 As shown. Figure 1 It can be seen that the in vitro antibacterial performance is as follows: Example 2>Comparative Example 1>Comparative Example 2>Comparative Example 3. In Comparative Example 1, no turmeric meal was added, and its antibacterial performance was weaker than that of Example 2; in Comparative Example 2, the amount of turmeric meal added was the same as that of Example 2, but the bacterial agent used was different from that of Example 2. The results showed that the antibacterial zone of Comparative Example 2 was smaller than that of Example 2, indicating that the fermentation effect of the bacterial agent used in the comparative example was lower than that of the present invention; the feed in Comparative Example 3 was not fermented, and the in vitro antibacterial performance was significantly lower than that of Example 2.

[0065] The results of the in vitro test with Escherichia coli as the indicator bacteria are as follows Figure 2 As shown. Figure 2 It can be seen that the in vitro antibacterial performance is as follows: Example 2>Comparative Example 1>Comparative Example 2=Comparative Example 3. In Comparative Example 1, no turmeric meal is added, and its antibacterial performance is weaker than that of Example 2; Comparative Examples 2 and 3 do not produce obvious antibacterial circles against Escherichia coli, indicating that the fermentation effect of the bacterial agent used in the comparative example is lower than that of the present invention.

[0066] In summary, Figure 1 and Figure 2 The test results fully demonstrate that the fermented turmeric meal feed prepared using the bacterial agent of the present invention has the best inhibitory effect on harmful bacteria.

[0067] Example 4 Experiment on the effect of fermented turmeric meal on laying hen production performance

[0068] CK: Basic daily diet (Jiale 623 laying hen feed produced by Tangrenshen Co., Ltd.)

[0069] T1: basic diet + fermented feed of Example 2, the mass ratio of the basic diet to the fermented feed of Example 2 is 16:1.

[0070] T2: basic diet + fermented feed of comparative example 1, the mass ratio of basic diet to fermented feed of comparative example 1 is 16:1.

[0071] T3: basic diet + fermented feed of comparative example 2, the mass ratio of basic diet to fermented feed of comparative example 2 is 16:1.

[0072] A total of 1080 healthy Hy-Line brown laying hens of 58 weeks of age and with an egg production rate of about 85% were selected and randomly divided into four treatment groups, with three replicates in each treatment and 90 hens in each replicate. The experiment lasted for 21 days.

[0073] The test material of this test case is designed based on the basic diet formula, and its nutritional ratio and basic diet feeding amount are implemented in accordance with the chicken feeding standard of the agricultural industry standard of the People's Republic of China "NY / T33-2004".

[0074] Test methods

[0075] Before the experiment, the inside and outside of the chicken house and all feeding utensils were cleaned and disinfected, and the chicken house was sealed and used after one week. CK, T1, T2, and T3 were fed in separate pens, and the mixing method was to mix the basic diet with the fermented feed in proportion and feed it immediately after mixing.

[0076] During the trial, a special person was assigned to be responsible for feeding. The experimental chickens were fed twice a day, and the recommended feed intake was about 115g, which was adjusted appropriately according to the actual situation. Free drinking water was provided. During the trial, the lighting was adjusted according to the feeding management regulations. The chicken house was well ventilated, disinfected regularly, and kept clean and hygienic. Health care was done to keep the laying hens in good health.

[0077] Evaluation indicators: egg production rate, egg weight, soft egg breakage rate, feed intake, feed-egg ratio, yolk color

[0078] The test results are shown in Table 1

[0079] Table 1 Determination of laying hen production indicators

[0080] Group CK T1 T2 T3 P-value Egg production rate% 85.81±1.85 86.73±3.02 86.59±1.35 85.87±2.05 0.371 Feed intake g 135.33±1.77 136.36±0.92 138.75±0.07 136.39±1.55 0.478 Egg weight g 61.51±0.54 62.84±0.40 62.22±0.57 62.28±0.02 0.047 Feed to egg ratio% 2.20±0.079 2.17±0.015 2.23±0.015 2.19±0.040 0.678 Soft broken egg rate % 0.607±0.163 0.310±0.104 0.427±0.127 0.587±0.086 0.017 Yolk color 9.16±0.31 10.01±1.72 9.45±0.47 9.21±0.92 0.019

[0081] From Table 1, we can see that:

[0082] 1. The use of the product of the embodiment of the present invention can significantly increase egg weight and improve egg production rate. The egg weight of TI increased by 2.16% compared with CK and increased by 0.90% compared with T3.

[0083] 2. The fermented feed prepared in Example 2 of the present invention was used to feed laying hens. The T1 result showed that the feed-egg ratio decreased from 2.20 to 2.17.

[0084] 3. In terms of reducing the soft egg breakage rate, the soft egg breakage rate of the poultry fed with Example 2 of the present invention was only 0.31%, and the CK and T3 mortality rates were both ≥0.5%, indicating that the fermented feed of the present invention can significantly reduce the egg breakage rate and improve the absorption and utilization rate of minerals in laying fowls.

[0085] 4. In terms of improvement of egg yolk color, the egg yolk color of laying hens fed with Example 2 of the present invention was significantly deepened, indicating that feeding the fermented turmeric meal of the present invention is more conducive to egg yolk coloring and improving egg quality.

[0086] Example 5 Experiment on the effect of fermented turmeric meal on the growth performance of broiler chickens

[0087] CK: Basic daily diet (Jia Le 613 broiler complete feed produced by Tangrenshen Co., Ltd.)

[0088] T1: basic diet + fermented feed of Example 2, the mass ratio of the basic diet to the fermented feed of Example 2 is 16:1.

[0089] T2: basic diet + fermented feed of comparative example 1, the mass ratio of basic diet to fermented feed of comparative example 1 is 16:1.

[0090] T3: basic diet + fermented feed of comparative example 2, the mass ratio of basic diet to fermented feed of comparative example 2 is 16:1.

[0091] A total of 2,000 healthy one-day-old yellow chickens with uniform body weight were randomly divided into four treatment groups, with five replicates in each group and 100 chickens in each replicate. The experiment lasted for 70 days.

[0092] The test material of this test case is designed based on the basic diet formula, and its nutritional ratio and basic diet feeding amount are implemented in accordance with the chicken feeding standard of the agricultural industry standard of the People's Republic of China "NY / T33-2004".

[0093] Test methods

[0094] Before the experiment, the inside and outside of the chicken house and all feeding utensils were cleaned and disinfected, and the chicken house was sealed and used after one week. CK, T1, T2, and T3 were fed in separate pens, and the mixing method was to mix the basic diet with the fermented feed in proportion and feed it immediately after mixing.

[0095] During the experiment, the chickens were raised by a dedicated person. The temperature was controlled within 28-35℃ and the relative humidity was controlled between 60% and 70% according to their age. The chickens were allowed to eat and drink freely every day, the chicken houses were naturally ventilated, and the pens were kept clean and sanitary.

[0096] Evaluation indicators: average daily weight gain, average daily feed intake, feed-to-meat ratio, mortality rate, slaughter rate, abdominal fat rate, and meat color.

[0097] The test results are shown in Table 2:

[0098] Table 2 Determination of broiler growth indexes

[0099] Group CK T1 T2 T3 P-value Average daily weight gain g 28.66±0.25 31.32±0.43 30.01±0.03 30.11±0.24 0.041 Average daily feed intake g 88.84±1.91 89.95±0.91 90.63±0.77 92.73±1.61 0.478 Feed to meat ratio 3.10±0.20 2.84±0.42 3.02±0.07 3.08±0.02 0.043 Slaughter rate % 92.24±0.33 92.77±0.29 91.79±0.54 91.91±0.24 0.858 Abdominal fat percentage 3.51±0.09 3.09±0.74 3.23±0.013 3.31±0.21 0.037 <![CDATA[Brightness L * > 48.01±3.31 50.01±2.32 49.91±2.37 48.81±1.92 0.123 <![CDATA[Red a * > 7.40±5.01 7.07±1.99 7.31±1.11 7.55±2.02 0.998 <![CDATA[Yellow b * > 9.26±1.21 12.74±5.32 10.97±4.87 10.66±5.02 0.008 Mortality rate % 1.60 0.20 0.20 1.50 —

[0100] From Table 2, we can see that:

[0101] 1. T1 can effectively improve the growth performance of yellow-feathered broilers. After using the product of the embodiment of the present invention, the average daily weight gain can be increased and the feed-to-meat ratio can be significantly reduced. TI is reduced by 8.38% compared with CK and by 7.7% compared with T3.

[0102] 2. The fermented feed prepared in Example 2 of the present invention was used to feed yellow-feathered broilers. The results of T1 showed that the average daily weight gain was about 31.32 g, which was 9.24% higher than that of CK and 4.01% higher than that of T3, and the improvement effect was quite obvious.

[0103] 3. In terms of reducing the mortality rate, the mortality rate of the yellow-feathered broilers fed with Example 2 of the present invention was only 0.2% during the test period, and the mortality rates of CK and T3 were both ≥1.5%, indicating that the fermented feed of the present invention has significant antibacterial and antiviral effects.

[0104] 4. In terms of improving meat quality, the abdominal fat rate of the yellow-feathered broilers fed with Example 2 of the present invention was significantly reduced, which was 0.42% lower than that of CK, the meat color brightness was improved, and the meat was more yellowish, indicating that feeding the fermented turmeric meal of the present invention is more conducive to pigment deposition and improves meat quality.

[0105] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A turmeric meal fermented feed, characterized in that: The turmeric meal fermented feed is made by fermenting the fermented base material with a fermentation agent; The fermentation base material is a crushed material of 30-80% by weight of turmeric meal, 5-20% by weight of soybean meal, 5-20% by weight of corn and 10-30% by weight of bran; The fermentation bacteria include Bacillus subtilis, Lactobacillus plantarum and yeast.

2. The fermented feed according to claim 1, characterized in that: The Bacillus subtilis is deposited in the General Microbiological Center of China Microorganism Culture Collection Administration CGMCC, and the deposit number is CGMCC NO.31665.

3. The method for preparing the fermented feed according to claim 1, characterized in that: The method comprises adding ferrous sulfate, zinc sulfate, manganese sulfate, complex enzyme and purified water to the fermentation base material of claim 1, inoculating a fermentation agent, mixing and fermenting to generate a fermented feed; The complex enzyme comprises alkaline protease, xylanase and cellulase.

4. The preparation method according to claim 3, characterized in that: The total amount of ferrous sulfate, zinc sulfate and manganese sulfate added is 0.4% of the weight of the fermentation base material, and the addition ratio of ferrous sulfate, zinc sulfate and manganese sulfate is 2:3:

3.

5. The preparation method according to claim 3, characterized in that: The addition amount of the complex enzyme is 0.2% of the weight of the fermentation base; the enzyme activity content ratio of protease, cellulose and xylanase in the complex enzyme is 1:1:1; the alkaline protease is 100,000 U / g of alkaline protease, the xylanase is 100,000 U / g of xylanase, and the cellulase is 8000 U / g of cellulase; the weight ratio of the pure water to the fermentation base is 10:(4-5).

6. The preparation method according to claim 3, characterized in that: The total inoculation amount of the Bacillus subtilis agent and the plant lactic acid bacteria agent in the fermentation agent is 0.4%-1%, the inoculation ratio is 1:1, and the yeast inoculation amount is calculated as active dry yeast, and the inoculation amount is 5×10 6 CFU / g.

7. The preparation process according to claim 3, characterized in that: The fermentation culture temperature is 35-37°C, the culture period is 3-7 days, and the effective viable bacteria count of the fermented feed is 5.0×10 8 -1.0×10 10 cfu / g.

8. Use of the turmeric meal fermented feed according to claim 1 or the fermented feed prepared by the preparation method according to claim 3 in feeding laying hens or broiler chickens; characterized in that: The fermented feed can be fed directly or made into granules or powder for feeding. The amount of turmeric meal fermented feed added in the feeding is 2%-10%.

9. Use of the turmeric meal fermented feed according to claim 1 or the fermented feed prepared by the preparation method according to claim 3 in improving the production performance of laying hens, eggshell quality or yolk color.

10. Use of the turmeric meal fermented feed according to claim 1 or the fermented feed prepared by the preparation method according to claim 3 in improving the meat quality of yellow-feathered broilers.

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