Composition for improving blood flow and composition for improving cold symptom

Abstract

The invention relates to a composition for improving blood flow and a composition for improving cold symptoms. Provided is a composition which promotes peripheral blood flow and has an excellent improvement effect on cold disorders. A composition for improving cold symptom, which contains spina date seed and perilla leaf as active ingredients.

Description

Technical Field

[0001] The present invention relates to a composition for improving blood flow and a composition having a cold symptom improving effect. Background Art

[0002] Cold syndrome is one of the self-reported symptoms caused by autonomic nervous system disorders, etc. It is a symptom confirmed in a wide range of generations from young to old, and is particularly common in women, with one in every two women said to suffer from cold syndrome.

[0003] It is believed that one of the main causes of cold syndrome is that stress and irregular lifestyle lead to decreased function of the autonomic nervous system, constriction of peripheral blood vessels, and decreased blood flow. In addition to organic symptoms such as headaches and back pain, cold syndrome may also cause systemic symptoms such as fatigue and decreased immunity.

[0004] Various studies have been conducted on ingredients that have the effect of improving cold symptoms. For example, cinnamon extract (Patent Document 1), black ginger rhizome extract (Patent Document 2), Indian gooseberry fruit (Patent Document 3), and piperine spike extract (Patent Document 4) have been reported to be effective in promoting peripheral blood flow and improving cold symptoms.

[0005] However, there is still a need for an ingredient that has a sufficient effect on improving cold symptoms.

[0006] Prior art literature

[0007] Patent Literature

[0008] Patent Document 1: Japanese Patent Application Publication No. 2009-106245

[0009] Patent Document 2: Japanese Patent Application Publication No. 2009-67731

[0010] Patent Document 3: Japanese Patent Application Publication No. 2006-335713

[0011] Patent Document 4: Japanese Patent No. 448266 Summary of the invention

[0012] Problem that the invention aims to solve

[0013] An object of the present invention is to provide a composition which promotes peripheral blood flow and has an excellent effect of improving cold symptoms.

[0014] Solutions for solving problems

[0015] The present inventors conducted intensive research to solve the above-mentioned problems and found that the combination of Chinese jujube seeds and perilla leaves showed a synergistic blood flow improvement effect. By taking these substances, excellent improvement effects can be obtained on cold symptoms, thereby completing the present invention.

[0016] That is, the present invention relates to a composition for improving cold symptoms, which contains spiny jujube seeds and perilla leaves as active ingredients.

[0017] In addition, the present invention relates to a composition for improving blood flow, which contains spiny jujube seeds and perilla leaves as active ingredients.

[0018] Effects of the Invention

[0019] The composition of the present invention has a synergistic blood flow promoting effect when ingested, thereby achieving an excellent cold symptom improving effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The GCPR test of Experimental Example 1 shows the effect of adenosine A on the single administration of Ziziphus jujuba seeds. 2A Graph of receptor agonist activity.

[0021] Figure 2 The results show that adenosine A is produced by administering perilla leaves alone in the GCPR test of Test Example 1. 2A Graph of receptor agonist activity.

[0022] Figure 3 The results show that adenosine A is produced by combining spiny jujube seeds and perilla leaves in the GCPR test of Test Example 1. 2A Graph of receptor agonist activity.

[0023] Figure 4 This is a graph showing the evaluation results of coldness of fingers based on VAS in Test Example 2.

[0024] Figure 5 This is a graph showing the evaluation results of coldness of the toes based on VAS in Test Example 2. DETAILED DESCRIPTION

[0025] The composition of the present invention uses spiny jujube seeds and perilla leaves as active ingredients.

[0026] The Chinese date seed is a crude drug based on the seeds of the rhamnaceae Chinese date (Ziziphus jujubaMiller var.spinosAHu exH.F.Chow). The Chinese date seed used in the present invention can be a crude drug directly, or a crude drug extract obtained by shredding or crushing the crude drug or extracting the crude drug with a solvent such as water or ethanol. The crude drug extract can be a concentrated soft extract or a dried extract powder.

[0027] Perilla leaf is a crude drug based on the dried leaves of Perilla frutescens Britton var. crispAW. Deane of the family Lamiaceae. The perilla leaf used in the present invention may be a crude drug directly, or may be a crude drug powder obtained by shredding or crushing the crude drug or extracting the crude drug with a solvent such as water or ethanol. The crude drug extract may be a concentrated soft extract or a dried extract powder.

[0028] The mass ratio of the Chinese jujube seeds and the perilla leaves in the composition of the present invention (in the case of a crude drug extract, the crude drug converted weight) is not particularly limited, but from the viewpoint of improving blood flow and improving cold symptoms, it is preferably 10:1 to 10.55:1 (Chinese jujube seeds: perilla leaves), and more preferably 10.13:1 to 10.42:1.

[0029] Adenosine receptors (A1, A 2A , A2B, A3) are widely distributed in the body and have a variety of physiological effects. Among them, adenosine A 2A When the receptors are activated, it will cause the dilation of coronary arteries or peripheral arteries and increase the blood flow of brain capillaries. 2A receptor agonist activity, but combined use of them can show synergistic adenosine A 2A The composition of the present invention has the activity of a receptor agonist, thereby dilating peripheral blood vessels and promoting blood flow, and can obtain an excellent effect of improving cold symptoms. The uses of the composition of the present invention include blood flow improvement uses, cold symptoms improvement uses, etc. There is no particular limitation on the parts of the blood flow improvement, for example, the extremities such as fingers and toes, the upper arm, the thigh, the brain, etc., but it is suitable for cold symptoms at the extremities such as fingers and toes. In the present invention, the above-mentioned improvement uses also include its preventive use.

[0030] The composition of the present invention includes medicines, quasi-drugs, foods, feeds, etc. for humans or animals. As dosage forms of medicines and quasi-drugs, for example, tablets, capsules, granules, powders, chewables, syrups, etc. can be cited. When preparing these preparations, in addition to the active ingredients of Semen Ziziphi Spinosae and Folium Perillae, pharmaceutically acceptable excipients, binders, extenders, disintegrants, surfactants, lubricants, dispersants, buffers, preservatives, flavoring agents, spices, coating agents, carriers, diluents, etc. can be appropriately combined as needed, and manufactured by conventional methods.

[0031] The dosage of the composition of the present invention is not particularly limited and can be appropriately set. For example, the daily dosage for an adult is about 1.7 to 2.0 g of Ziziphus jujuba seeds and 0.16 to 0.20 g of Perilla frutescens leaves (in the case of a crude drug extract, the crude drug equivalent weight).

[0032] As the form when the composition of the present invention is made into food, except various foods such as food or nutritious food such as jelly, hard candy, soft candy, gummy candy (gummycandy), chewing gum, wafer biscuit, biscuit (biscuits), chocolate, bread, noodles, sausage, etc., it is also possible to enumerate the nutritional supplement composition of the same form (tablet, capsule, chewing agent, syrup, etc.) as the above-mentioned medicines, etc. When preparing these foods, except as the Chinese date seed and perilla leaf of effective ingredients, other food materials or sweet seasoning, gelling agent, developer, mildewproof agent, acid seasoning, seasoning, pH adjusting agent, swelling agent, solvent, softener, oil, emulsifier, preservative, aromatic family, stabilizer, coloring agent, antioxidant, humectant, thickener, etc. can also be appropriately combined as required, and manufacture according to a conventional method. Food includes functional food, specific health food, functional expression food, etc.

[0033] The composition of the present invention may be accompanied by a display for preventing / improving cold symptoms, improving a constitution that is prone to catching cold, improving blood flow and maintaining body temperature, dilating blood vessels and maintaining blood flow, or promoting blood circulation. The act of accompanying the display includes: recording it on the body, container, package, etc. of the product containing the composition of the present invention; recording it in documents such as instructions, attachments, brochures, and other printed materials that disclose product information; recording it in advertisements used for promotion including various leaflets and the Internet; etc.

[0034] The present invention will be described in more detail below with reference to Examples and Test Examples, but the present invention is not limited to these Examples and the like.

[0035] Example

[0036] Test Example 1

[0037] Adenosine A 2A Receptor agonist activity test:

[0038] The reporter cells were cultured in DMEM supplemented with 10% FCS and penicillin / streptomycin. The cells were freed using trypsin-EDTA and suspended in DMEM containing 10% dialyzed FCS. The suspended cells were seeded in a 96-well white microplate at 24,000 cells per well. The cells were cultured at 37°C for 1 night in the presence of 5% CO2 to allow the seeded cells to adhere to the bottom of the microplate.

[0039] Plasmids expressing GPCR, Gα protein, and luciferase reporter gene were mixed, and a total of 100 ng was transfected per well using a transfection reagent (FuGENE6, Promega), and cultured at 37°C overnight in the presence of 2.5% CO2.

[0040] 2 ml of DMSO was added to 180 mg of each of the powder of jujube seeds, perilla leaves, and mixed powder of jujube seeds and perilla leaves, and the solution was homogenized by ultrasonic treatment. After centrifugation (10,000 rpm, 10 minutes), the supernatant was recovered and filtered (0.45 μm) to prepare a crude drug extract.

[0041] The crude drug extract was diluted to 7 times the final test concentration using DMEM containing 10% dialyzed FCS, added to each well, and cultured at 37°C for 6 hours in the presence of 2.5% CO2. After aspirating the culture medium, a luciferase quantitative reagent (Steady-Glo, Promega) was added to the wells, and the luminescence of luciferase was measured using a microplate reader (Victor Nivo, Perkin Elmer).

[0042] Data analysis The Hill equation was approximated by nonlinear least squares method. The parameters were set as EC50, Hill coefficient, curve bottom, and curve top. The results are shown in Table 1. Figures 1 to 3 .

[0043] [Number 1]

[0044]

[0045] [Table 1]

[0046] Hill coefficient EC50(μg / mL) EC80(μg / mL) Chinese date seed 1.3 130 392 Perilla leaves 1.4 126 341 Ziziphus jujuba seeds + Perilla leaves 2.4 106 191

[0047] From these results, it was confirmed that the combination of wild jujube seeds and perilla leaves increased the Hill coefficient, which is a reactivity index, by about 80% compared with the use of each alone, showing a synergistic effect.

[0048] Example 1

[0049] Preparation of tablets containing spiny jujube seeds and perilla leaves

[0050] Add 10 times the amount of purified water to the cut raw materials of Chinese jujube seeds and Chinese perilla leaves, heat to 95-100°C under stirring, and extract for about 60 minutes. After the extraction, separate the extract into solid and liquid, concentrate the separated liquid under reduced pressure, and then spray dry to obtain dry Chinese jujube seeds and perilla leaves extract powder.

[0051] The obtained Ziziphus jujuba seed and Perilla leaf extract powders are mixed with lactose, microcrystalline cellulose and magnesium stearate, and tableted using a tablet press to produce tablets containing 1.85 g of Ziziphus jujuba seed and 0.18 g of Perilla leaf in terms of crude drug weight per 3 tablets (containing 52 mg in total as Ziziphus jujuba seed extract powder and Perilla leaf extract powder).

[0052] Test Example 2

[0053] Human trials

[0054] Thirty healthy women aged 35 to 50 were selected as subjects in each group. The tablets prepared in Example 1 were taken once a day for 12 weeks, and VAS (Visual Analogue Scale) was used to evaluate "coldness of fingers" and "coldness of toes" 4, 8, and 12 weeks after the start of intake (herbal medicine group). As a control, tablets without the extract powder of Ziziphus jujuba and Perilla leaf were taken and evaluated in the same way using VAS (control group). The results are shown in Figure 4 and Figure 5 .

[0055] As shown in this result, it was confirmed that the coldness of the extremities such as the hands, feet, and toes can be significantly improved by taking the wild jujube seeds and the perilla leaves together, and the effect is enhanced over time.

[0056] Industrial Applicability

[0057] By taking the composition of the present invention, peripheral blood flow is promoted and the effect of improving cold symptoms can be obtained, and it is useful as a medicine, food, etc. for improving cold symptoms.

Claims

1. A composition for improving cold symptoms, comprising Ziziphus jujuba seeds and Perilla leaves as active ingredients.

2. The composition for improving cold symptoms according to claim 1, wherein Cold syndrome refers to coldness in the fingers or toes.

3. A composition for improving blood flow, comprising Ziziphus jujuba seeds and Perilla frutescens leaves as active ingredients.

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