Skin-friendly fabric, sanitary napkin, method and application for simultaneously and continuously releasing vitamin B5, vitamin C and collagen peptide

Abstract

The present invention relates to the technical field of sanitary napkins, and in particular to skin-friendly fabrics, sanitary napkins, methods and applications that simultaneously and continuously release vitamin B5, vitamin C and collagen peptides. The present invention obtains a skin-friendly fabric by treating a nano slow-release composition loaded with vitamin B5, vitamin C and collagen peptides into pure cotton fabric, and prepares a sanitary napkin using the skin-friendly fabric as an inner layer material. The skin-friendly fabric and the sanitary napkin have the function of simultaneously and slowly and continuously releasing vitamin B5, vitamin C and collagen peptides, and can continuously maintain the protective effects of vitamin B5, vitamin C and collagen peptides on vaginal health, and maintain vaginal health in a long-term manner.

Description

Technical Field

[0001] The present invention relates to the technical field of sanitary napkins, and particularly to a skin-friendly fabric, a sanitary napkin, a method and an application for simultaneously and continuously releasing vitamin B5, vitamin C and collagen peptides. Background Art

[0002] A sanitary napkin is a female sanitary product with good absorbency. The main materials are cotton, non-woven fabric, pulp or a high molecular polymer and a high molecular polymer composite paper formed by the above material composites. The design of the side is mainly used to prevent side leakage. The back glue of the sanitary napkin is a water-impermeable material, which can retain menstrual blood in the sanitary napkin and is used to absorb the menstrual blood flowing out of the vagina during a woman's menstrual period. The surface layer of the existing sanitary napkin is generally a non-woven fabric or a cotton glue mixed layer, with a relatively low overall structural cost and a relatively simple assembly process. Summary of the Invention

[0003] In view of this, the present invention provides a sanitary napkin for simultaneously and continuously releasing vitamin B5, vitamin C and collagen peptides and a preparation method thereof. The present invention obtains a skin-friendly fabric by treating a nano-sustained release composition loaded with vitamin B5, vitamin C and collagen peptides into pure cotton fabric, and uses the skin-friendly fabric as an inner layer material to prepare a sanitary napkin. The skin-friendly fabric and the sanitary napkin have the function of simultaneously and slowly releasing vitamin B5, vitamin C and collagen peptides, and can continuously maintain the protective effect of vitamin B5, vitamin C and collagen peptides on vaginal health, and long-term maintain vaginal health.

[0004] One object of the present invention is to provide a preparation method of a skin-friendly fabric, including: preparing a nano-sustained release composition, the nano-sustained release composition being calcium carbonate particles encapsulating vitamin B5, vitamin C and collagen peptides; treating pure cotton fabric with glutaraldehyde; and laying the nano-sustained release composition between two layers of pure cotton fabric treated with glutaraldehyde, and obtaining the skin-friendly fabric by rolling.

[0005] Specifically, the steps of preparing the nano-sustained release composition include: adding sodium polyaspartate to a calcium chloride solution, and after reaction, dropping a sodium carbonate solution; after the dropping is completed, adding an inclusion complex solution, performing ultrasonic treatment, and continuously performing adsorption treatment under stirring; after the adsorption is completed, centrifuging the solution, washing with ethanol and centrifuging, and freeze-drying to constant weight to obtain the nano-particles; preparing an aqueous solution containing the nano-particles and reacting with stearic acid at room temperature for at least 48 h by wet method, washing with ethanol and centrifuging, and freeze-drying to constant weight to obtain the nano-sustained release composition.

[0006] Specifically, the steps for preparing the nano-sustained release composition specifically include: taking 20 mL each of 0.1 M sodium carbonate solution and 0.1 M calcium chloride solution, adding 666 mg of sodium polyaspartate to the calcium chloride solution and stirring for crosslinking for 30 min, and then dropwise adding the sodium carbonate solution to the calcium chloride solution added with sodium polyaspartate at a rate of 10 mL / min under stirring; after the dropping is completed, adding 50 mL of 30 mg / mL vitamin B5 aqueous solution, or an aqueous solution containing 10 mg / mL vitamin C and 30 mg / mL vitamin B5, or a suspension containing 5 mg / mL linseed collagen peptide, 10 mg / mL vitamin C and 30 mg / mL vitamin B5 and ultrasonicating for 30 min; then continuously adsorbing at a rotation speed of 1000 rpm for 24 h; after the reaction is completed, centrifuging the suspension at a rotation speed of 12000 rpm for 30 min, washing with ethanol, centrifuging, and then freeze-drying to constant weight to obtain nanoparticles; mixing an aqueous solution containing 80 wt% nanoparticles with stearic acid, standing at room temperature for 72 h after wet method, taking out, centrifuging at a rotation speed of 12000 rpm for 30 min, washing with ethanol, centrifuging, and then freeze-drying to constant weight to obtain the nano-sustained release composition.

[0007] Specifically, the addition amount of stearic acid is 1.0 - 1.25% of the mass of the nanoparticles.

[0008] One of the purposes of the present invention is to provide a preparation method of the sanitary napkin. The method includes: preparing a nano-sustained release composition, the nano-sustained release composition being calcium carbonate particles encapsulating vitamin B5, vitamin C and collagen peptide; treating pure cotton fabric with glutaraldehyde; laying the nano-sustained release composition between two layers of pure cotton fabric treated with glutaraldehyde, and obtaining a skin-friendly fabric through rolling; sewing the skin-friendly fabric or a plurality of the skin-friendly fabrics as the inner layer material into the outer layer material to obtain the sanitary napkin.

[0009] Specifically, the sanitary napkin includes an inner layer facing the body surface and an outer layer that is liquid-proof against leakage towards the clothing surface; wherein, the inner layer is sewn with a skin-friendly fabric containing vitamin B5, and the inner layer and the outer layer are sewn into a whole.

[0010] Specifically, the outer layer extends outward to completely cover the inner layer and extends outward to have two wings; wherein, the dividing part between the inner layer and the outer layer is sewn with elastic spandex filaments to divide the wings of the inner layer and the outer layer, and the inner layer is sewn at the middle position of the outer layer.

[0011] Specifically, elastic spandex filaments are embedded at the edges of both the inner layer and the outer layer.

[0012] Specifically, the inner layer includes multiple layers of the skin-friendly fabric.

[0013] Specifically, the treatment of pure cotton fabric with glutaraldehyde specifically includes: taking 200 mL of a glutaraldehyde aqueous solution with a concentration of 0.5 wt% and a pH of 5, adding 4 g of magnesium chloride crystals, stirring until completely dissolved, and then adding 200 cm 2 of high-count gauze. Finally, place the conical flask in a constant-temperature oscillator and treat it in a constant-temperature water bath oscillator at 60 °C for 1 h. Then take out the high-count gauze, wash or soak it in deionized water for 3 h, and then take it out and roll it with a mangle, with a liquor pick-up rate of 60%; wash it again with deionized water until no glutaraldehyde is detected in the residual liquid.

[0014] Specifically, the rolling specifically includes: laying the nano-sustained release composition at a ratio of 50 mg / cm 2 between two layers of pure cotton fabric treated with glutaraldehyde; taking it out and rolling it with a mangle, with a liquor pick-up rate of 60%; soaking it in deionized water for 3 h again, taking it out and rolling it with a mangle, with a liquor pick-up rate of 60%, and drying it at room temperature to obtain the skin-friendly fabric.

[0015] Application of the skin-friendly fabric provided by one of the purposes of the present invention in the preparation of sanitary napkins.

[0016] Beneficial effects of the present invention:

[0017] By preparing a nano-sustained release composition encapsulating vitamin B5, vitamin C and collagen peptides and rolling it into the treated pure cotton fabric, the present invention further improves the sustained release effect compared with the nano-sustained release composition, can continuously release vitamin B5, vitamin C and collagen peptides, and can continuously maintain the protective effect of vitamin B5, vitamin C and collagen peptides on vaginal health, and long-term maintain vaginal health.

[0018] By preparing a nano-sustained release composition and rolling it into the treated high-count gauze, the present invention obtains a skin-friendly fabric with closeness and comfort. This skin-friendly fabric can be used to prepare the inner layer directly facing the body surface and the sanitary napkin composed of the inner layer. It can not only achieve the effect of being close to the skin, but also avoid the generation of gaps between the inner layer and the human body when the user is in a moving state, improve the closeness of the inner layer, and thus ensure the absorption effect. Moreover, due to the function of continuously releasing vitamin B5, vitamin C and collagen peptides of this skin-friendly fabric, the inner layer made of this skin-friendly fabric can not only maintain the integrity of the cell membrane of vaginal mucosal cells, effectively resist the invasion of external pathogens, and maintain the balance of the vaginal microbial community.

[0019] Through cell experiments, the present invention finds that this skin-friendly fabric not only has no obvious toxic and side effects on vaginal mucosal epithelial cells, but also can maintain the integrity of the cell membrane of vaginal mucosal cells, effectively resist the invasion of external pathogens, and maintain the balance of the vaginal microbial community.

[0020] Through cell experiments, it is found in the present invention that the skin-friendly fabric and the sanitary napkin prepared based on it can not only activate the NLRP3 inflammasome to a certain extent within 12 hours to initiate an immune response against Candida albicans, but also prevent the lysis of vaginal epithelial cells due to the inflammatory response after 48 hours and prevent damage to the vaginal mucosa. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 Loss rate of vitamin B5 in steps 1) - 3) during the preparation of skin-friendly fabric 1.

[0022] Figure 2 Loss rates of vitamin B5 and vitamin C in steps 1) - 3) during the preparation of skin-friendly fabric 2.

[0023] Figure 3 Loss rates of vitamin B5, vitamin C and collagen peptide in steps 1) - 3) during the preparation of skin-friendly fabric 3.

[0024] Figure 4 Loss rate of vitamin B5 during the water wash resistance test of skin-friendly fabrics 1 - 5.

[0025] Figure 5 Loss rates of vitamin C during the water wash resistance test of skin-friendly fabrics 2 - 5.

[0026] Figure 6 Loss rates of collagen peptide during the water wash resistance test of skin-friendly fabrics 3 - 5.

[0027] Figure 7 Cumulative release rate of vitamin B5 of vitamin B5 powder and sanitary napkins 1 - 5 in vaginal simulation fluid.

[0028] Figure 8 Cumulative release rate of vitamin C of vitamin C powder and sanitary napkins 2 - 5 in vaginal simulation fluid.

[0029] Figure 9 Cumulative release rate of collagen peptide of linseed collagen peptide product and sanitary napkins 3 - 5 in vaginal simulation fluid.

[0030] Figure 10 Results diagram of the effect of groups 1 - 5 on the activity of rabbit primary vaginal mucosal epithelial cells.

[0031] Figure 11 Results diagram of the LDH release rate of groups 1 - 5 on rabbit primary vaginal mucosal epithelial cells.

[0032] Figure 12 Results diagram of the 12h NLRP3 expression of groups 1 - 5 on rabbit primary vaginal mucosal epithelial cells treated with Candida albicans.

[0033] Figure 13 The figure shows the NLRP3 expression results of rabbit primary vaginal mucosal epithelial cells treated with Candida albicans in groups 1-5 for 48 hours.

[0034] Figure 14 The figure shows the schematic structure of the sanitary napkin provided by the present invention.

[0035] Figure 15 The figure shows the schematic side view of the sanitary napkin provided by the present invention. Detailed implementation manners

[0036] In order to make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below in combination with groups. It should be understood that the specific groups described herein are only used to explain the present invention and are not used to limit the present invention. The reagents not described in detail and separately in the present invention are all conventional reagents and can be obtained through commercial channels; the methods not described in detail and specifically are all conventional experimental methods and can be learned from the prior art.

[0037] Example 1: Preparation of skin-friendly fabric

[0038] 1) Preparation of nano-sustained release composition

[0039] Take 20 mL each of 0.1 M sodium carbonate solution and 0.1 M calcium chloride solution. Add 666 mg of sodium polyaspartate to the calcium chloride solution and stir for crosslinking for 30 min. Then, dropwise add the sodium carbonate solution to the calcium chloride solution added with sodium polyaspartate at a rate of 10 mL / min under stirring. After the addition is completed, add 50 mL of 30 mg / mL vitamin B5 aqueous solution and ultrasonicate for 30 min, and then continuously adsorb at a rotation speed of 1000 rpm for 24 h. After the reaction is completed, centrifuge the suspension at a rotation speed of 12000 rpm for 30 min, wash with ethanol, centrifuge, and then freeze-dry to constant weight to obtain nanoparticles;

[0040] Prepare an aqueous solution containing 80 wt% of nanoparticles, then add 1.25% of stearic acid based on the mass of the nanoparticles. Take it out after standing at room temperature for 72 h by the wet method, centrifuge at a rotation speed of 12000 rpm for 30 min, wash with ethanol, centrifuge, and then freeze-dry to constant weight to obtain the nano-sustained release composition.

[0041] The average particle size of the nano-sustained release composition in the suspension after the reaction was measured by a nano laser particle size analyzer to be 369 nm, and the polydispersity index was 0.142.

[0042] 2) Treat the pure cotton fabric with glutaraldehyde

[0043] Take 200 mL of 0.5 wt% glutaraldehyde aqueous solution with a pH of 5, add 4 g of magnesium chloride crystals, stir to completely dissolve it, and then add 200 cm 2High-count gauze (80S, density specification 120×80), after being treated in a constant temperature water bath shaker at 60°C for 1 h, take out the high-count gauze, wash or soak it in deionized water for 3 h, then take it out and roll it with a mangle, with a liquor pickup rate of 60%. Wash it again with deionized water until no glutaraldehyde is detected in the residual liquid.

[0044] 3) Rolling

[0045] Lay the nano-sustained release composition obtained in step 1) at a ratio of 50 mg / cm 2 between two layers of pure cotton fabrics obtained in step 2). Take it out and roll it with a mangle, with a liquor pickup rate of 60%. Soak it in deionized water again for 3 h, take it out and roll it with a mangle, with a liquor pickup rate of 60%, and dry it at room temperature to obtain the skin-friendly fabric 1.

[0046] Furthermore, in the above step 1), prepare the nano-sustained release composition with an aqueous solution containing 10 mg / mL vitamin C and 30 mg / mL vitamin B5, and the other steps are the same as the above examples to prepare the skin-friendly fabric 2.

[0047] Furthermore, in the above step 1), prepare a suspension containing 5 mg / mL linseed collagen peptide (Jinmu Group), 10 mg / mL vitamin C and 30 mg / mL vitamin B5, and prepare the nano-sustained release composition after ultrasonic re-dispersion. The other steps are the same as the above examples to prepare the skin-friendly fabric 3.

[0048] Calculate the loss rate of vitamin B5, vitamin C and collagen peptide in each step. Among them, the loss rate of vitamin B5 in step 1) is the percentage of the weight of vitamin B5 in the nano-sustained release composition suspension to the total weight of the input vitamin B5. The loss rate of vitamin B5 in step 2) is the percentage of the weight of vitamin B5 in the washing aqueous solution to the total weight of the input vitamin B5. The loss rate of vitamin B5 in step 3) is the percentage of the weight of vitamin B5 in the elution aqueous solution to the total weight of the input vitamin B5.

[0049] As Figure 1 shown, the loss rate of vitamin B5 in steps 1) to 3) of the preparation process of the skin-friendly fabric 1 decreases successively, and finally the nano-sustained release composition is stably rolled between the pure cotton fabrics to obtain the skin-friendly fabric 1.

[0050] As Figure 2 shown, the loss rates of vitamin B5 and vitamin C in steps 1) to 3) of the preparation process of the skin-friendly fabric 2 decrease successively, and finally the nano-sustained release composition is stably rolled between the pure cotton fabrics to obtain the skin-friendly fabric 2.

[0051] As Figure 3As shown, the loss rates of pantothenic acid, vitamin C, and collagen peptides in steps 1) to 3) of the preparation process of the skin-friendly fabric 3 decrease successively. Although the loss of collagen peptides in step 1) is relatively large, the nano-sustained release composition is finally stably rolled between pure cotton fabrics, thereby obtaining the skin-friendly fabric 3.

[0052] Comparative Example 1: Preparation of skin-friendly fabric

[0053] Referring to CN112334101A, a material formed by wrapping a mixture of pulp fibers and superabsorbent polymers with two layers of cotton paper on the top and bottom (unit area weight: 200 g / m 2 , the mass ratio of pulp fibers to superabsorbent polymers is 10:1, and the thickness is 2.0 mm) constitutes an absorbent body. A suspension containing 5 mg / mL linseed collagen peptides (Jinmu Group), 10 mg / mL vitamin C, and 30 mg / mL pantothenic acid is coated on the absorbent body at a coating amount of 50 mg / cm 2 . The two layers of absorbent bodies are laminated, the expression rate is 60%, and it is air-dried at room temperature to prepare the skin-friendly fabric 4.

[0054] Comparative Example 2: Preparation of skin-friendly fabric

[0055] Referring to CN112334101A, a material formed by wrapping a mixture of pulp fibers and superabsorbent polymers with two layers of cotton paper on the top and bottom (unit area weight: 200 g / m 2 , the mass ratio of pulp fibers to superabsorbent polymers is 10:1, and the thickness is 2.0 mm) constitutes an absorbent body. The same mass of linseed collagen peptides, vitamin C, and pantothenic acid raw materials as those contained in the skin-friendly fabric 3 are laminated between the two layers of absorbent bodies by pressing. It is taken out and rolled by a rolling mill, the expression rate is 60%, and it is air-dried at room temperature to obtain the skin-friendly fabric 5.

[0056] Test Example 1: Wash resistance test of skin-friendly fabric

[0057] The skin-friendly fabrics 1 to 5 prepared in the above Example 1, Comparative Example 1, and Comparative Example 2 were respectively subjected to a wash resistance test using a household washing machine. The washing program was 4N, that is, washing at 40 °C for 15 min and hanging to dry. Referring to the above method, the loss rates of pantothenic acid, vitamin C, and collagen peptides in the skin-friendly fabrics 1 to 5 before and after washing were calculated.

[0058] As Figure 4 shown, the loss rate of pantothenic acid in the skin-friendly fabrics 1 to 3 is significantly lower than that in the skin-friendly fabrics 4 and 5. As Figure 5 shown, the loss rate of vitamin C in the skin-friendly fabrics 2 to 3 is significantly lower than that in the skin-friendly fabrics 4 and 5. As Figure 6 shown, the loss rate of collagen peptides in the skin-friendly fabric 3 is significantly lower than that in the skin-friendly fabrics 4 and 5.

[0059] This shows that the skin-friendly fabric 1-3 prepared by the method provided by the present invention can enable pantothenic acid, vitamin C and collagen peptides to form a deep binding effect with the pure cotton fabric. For example, a covalent bond binding effect is formed on the surface of cellulose, which can prevent pantothenic acid, vitamin C and collagen peptides from being easily released from the surface of the pure cotton fabric. Compared with the ordinary coating methods of Comparative Examples 1 and 2, the binding is tighter and the washing resistance is stronger, providing a basis for its repeated use. In addition, due to its deep binding effect with the pure cotton fabric, it provides a basis for its preparation into a sanitary napkin to continuously release and produce a bioactive effect in an environment containing secretions and excreta at the vaginal orifice.

[0060] Example 2: Preparation of sanitary napkin

[0061] See Figure 14 、 Figure 15 A sanitary napkin of a specific embodiment includes an inner layer 12 facing the body surface and an outer layer 11 that is liquid-proof on the surface facing the clothing. Among them, the inner layer 12 is sewn from the skin-friendly fabric provided in the above Example 1. The inner layer 12 and the outer layer 11 are sewn into a whole.

[0062] Among them, the outer layer 11 extends outward to completely cover the inner layer 12 and extends outward to have two wings 120 to prevent side leakage. Among them, the entire inner layer 12 is sewn with the skin-friendly fabric. The dividing part between the inner layer 12 and the outer layer is sewn with elastic spandex 113 and divides the wings 120 of the inner layer 12 and the outer layer 11. The inner layer 12 is roughly sewn in the middle position of the outer layer 11. Further, elastic spandex is embedded at the edges of both the inner layer and the outer layer.

[0063] In a preferred embodiment, as Figure 14 ,the inner layer 12 includes multiple layers of the skin-friendly fabric 1-skin-friendly fabric 5 provided in the above Example 1, such as 2-5 layers, and the inner layer 12 can be formed by further pressing or sewing.

[0064] The sanitary napkin designed in this way can not only achieve the effect of being close to the skin, but also prevent gaps from being generated between the inner layer 12 and the human body when the user is in a moving state, improve the closeness of the inner layer 12 to the body, and thus ensure the absorption effect.

[0065] During implementation, the material of the outer layer 11 can be a waterproof and breathable micro-nano fiber non-woven fabric film, a composite of ordinary PP non-woven fabric, polyethylene non-woven fabric, etc. An adhesive layer can be provided on the lower surface of the outer layer 11 to facilitate bonding and fixing to the inner side of a female's underwear; the material of the upper surface layer 114 of the outer layer 11 can be a skin-friendly non-woven fabric made of skin-friendly PP material or a skin-friendly cotton cloth with a cotton texture. The material of the lower surface of the outer layer 11 can be a skin-friendly non-woven fabric, a skin-friendly cotton cloth with a cotton texture, a micro-nano fiber non-woven fabric film, a composite of ordinary PP non-woven fabric, etc. The four peripheries of the upper and lower surface layers are fixedly connected by a high-temperature pressing method. The spandex 113 has the reputation of the second skin of the human body, is safe and comfortable to use, has good elasticity, and can also be replaced by other skin-friendly elastic flexible threads during manufacturing, and is embedded inside the corresponding peripheries when the four peripheries of the upper and lower surface layers are pressed and connected.

[0066] Example 3: Simulation test of sanitary napkins

[0067] Prepare a vaginal simulation fluid according to the method of "Owen DH, Katz DF. A vaginal fluid stimulant [J]. Contraception, 1999, 59(2): 91-95". Use the skin-friendly fabrics 1-5 prepared in Example 1 above as raw materials to prepare sanitary napkins 1-5 respectively. Immerse the sanitary napkins 1-5 completely in the vaginal simulation fluid for 24 hours. Use conventional methods to detect the contents of vitamin B5, vitamin C, and collagen peptide in the solution after soaking, and calculate the cumulative release rates of vitamin B5, vitamin C, and collagen peptide in the sanitary napkins 1-5. The cumulative release rate is the percentage of the mass of vitamin B5, vitamin C, and collagen peptide in the solution after soaking in the total weight input in the sanitary napkins 1-5. In addition, directly put the same mass of vitamin B5 powder into the vaginal simulation fluid to test the cumulative release rate in the solution. In addition, directly put the same mass of vitamin C powder into the vaginal simulation fluid to test the cumulative release rate in the solution. In addition, directly put the linseed collagen peptide product into the vaginal simulation fluid to test the cumulative release rate in the solution.

[0068] As Figure 7 shown, the cumulative release rates of vitamin B5 in sanitary napkins 4 and 5 are significantly higher than those in sanitary napkins 1-3 in the first 14 hours. As Figure 8 shown, the cumulative release rates of vitamin C in sanitary napkins 4 and 5 are significantly higher than those in sanitary napkins 2 and 3 in the first 18 hours. As Figure 9 shown, the cumulative release rates of collagen peptide in sanitary napkins 4 and 5 are significantly higher than those in sanitary napkins 2 and 3 in the first 16 hours.

[0069] This shows that the sanitary napkins 1-3 prepared by the present invention are respectively prepared by using the skin-friendly fabrics 1-3. The skin-friendly fabrics 1-3 can enable pantothenic acid, vitamin C and collagen peptides to form a deep binding effect with the pure cotton fabric, so that pantothenic acid, vitamin C and collagen peptides will not be easily released from the surface of the pure cotton fabric, and their sustained release effect in the vaginal simulation fluid is obvious. The skin-friendly fabrics 4 and 5 only adopt the ordinary coating method, and their sustained release effect is inferior to that of the sanitary napkins 1-3 prepared by using the skin-friendly fabrics 1-3 provided by the present invention.

[0070] Example 4: Influence of skin-friendly fabric on vaginal epithelial cells

[0071] 1. Cell line and cell culture medium

[0072] Primary rabbit vaginal mucosal epithelial cells (Bio-137458, purchased from Beijing BioVector NTCC Inc.) were cultured in keratinocyte medium (Gibco, USA) at 37°C and 5% CO 2 Under the condition, the cells grew in a monolayer adherent manner, and the cells in the logarithmic phase were taken for experiments.

[0073] 2. Influence on cell viability

[0074] The primary rabbit vaginal mucosal epithelial cells in the logarithmic growth phase were collected and inoculated into a 96-well cell culture plate, with about 5000 cells inoculated in each well. After the cells adhered, the culture medium was aspirated, washed once with PBS, and divided into groups 1, 2, 3, 4, and 5.

[0075] After adding the skin-friendly fabric 3 with a diameter of 3 mm to group 1, 200 μL of culture medium was added, and the cells were cultured for 24 h. Then, 20 μL of MTT working solution (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide working solution) was added, and the cells were cultured for 4 h. After centrifugation, the supernatant was discarded, 150 μL of dimethyl sulfoxide was added to dissolve the purple crystals, and the absorbance was measured at 490 nm with an enzyme-linked immunosorbent assay (ELISA) reader.

[0076] After adding the nano-sustained release composition with the same mass as that included in the skin-friendly fabric 3 with a diameter of 3 mm to group 2, the test steps were the same as those in group 1.

[0077] After adding the skin-friendly fabric 4 with a diameter of 3 mm to group 3, the test steps were the same as those in group 1.

[0078] After adding the same mass of vitamin C, pantothenic acid and collagen peptides as those included in the skin-friendly fabric 4 with a diameter of 3 mm to group 4, the test steps were the same as those in group 1.

[0079] After adding the skin-friendly fabric 5 with a diameter of 3 mm to group 5, the test steps were the same as those in group 1.

[0080] In addition, a control group was set up without adding the skin-friendly fabric or the nano-sustained release composition. The absorbance was measured at 490 nm, and the percentage of the difference in the absorbance measured at 490 nm in each group compared to the control group was calculated, which was the cell survival rate.

[0081] It can be seen from Figure 10 that the skin-friendly fabrics 1-5 and the nano-sustained release compositions of each group have no obvious toxic and side effects on rabbit primary vaginal mucosal epithelial cells.

[0082] 3. Effect on cell LDH (lactate dehydrogenase) release

[0083] Take 120 μL of the supernatant of the cells treated in each group above after 4 h and add it to the corresponding wells of a new 96-well plate. Add 60 μL of LDH working solution (lactate dehydrogenase cytotoxicity detection kit, Beyotime Biotechnology Research Institute), incubate in the dark at room temperature for 0.5 h, detect with an enzyme-linked immunosorbent assay (ELISA) reader, measure the absorbance at 490 nm, and calculate the percentage of the difference in the absorbance measured at 490 nm in each group compared to the control group, which is the cell LDH release rate.

[0084] It can be seen from Figure 11 that the LDH release rates of each group are relatively low, especially in groups 1 and 2. Under normal circumstances, LDH exists in the cytoplasm and cannot penetrate the cell membrane. When the cells are damaged, the cell membrane permeability changes, and LDH can be released from the cell interior to the extracellular space. This indicates that the skin-friendly fabric, nano-sustained release composition, vitamin C, vitamin B5, and collagen peptide provided by the present invention have a relatively low LDH release rate, indicating that their action on rabbit primary vaginal mucosal epithelial cells does not cause a change in the cell membrane permeability, that is, they have no obvious toxic and side effects on rabbit primary vaginal mucosal epithelial cells, and can not only maintain the integrity of the cell membrane of vaginal mucosal cells, but also effectively resist the invasion of external pathogens and maintain the balance of the vaginal microbial community.

[0085] Example 5: Regulatory effect of the skin-friendly fabric on NLRP3 inflammasome (NOD-like receptor protein 3 (NLRP3) inflammasome)

[0086] 1. Co-culture of vaginal epithelial cells and Candida albicans

[0087] Rabbit primary vaginal mucosal epithelial cells (Bio-137458, purchased from Beijing BioVector NTCC Inc.) were cultured in keratinocyte medium (Gibco, USA) at 37 °C and 5% CO 2 Under these conditions, the cells grew in a monolayer adherent manner. Logarithmic-phase cells were inoculated into a 96-well cell culture plate, with about 5000 cells inoculated in each well. After the cells adhered, the culture medium was aspirated, and the cells were washed once with PBS. The blank group, model group, groups 1, 2, 3, 4, and 5.

[0088] The model group was added with 200 μL of Candida albicans prepared with K-SFM culture medium (keratinocyte serum-free medium) at 1×10 6 CFU / mL ( Candida albicans , CMCC(F)98001, CMCC) at 6, 12, 24, and 48 h of culture respectively, and cultured in an incubator at 37°C and 5% CO 2 . After 48 h, the supernatant was collected simultaneously and stored in the dark at -80°C. The cells in the 24-well plate were washed with PBS and then fixed with 4% paraformaldehyde and stored in the refrigerator at 4°C.

[0089] After adding the skin-friendly fabric 3 with a diameter of 3 mm to group 1, 200 μL of Candida albicans prepared with K-SFM culture medium at 1×10 6 CFU / mL was added at 6, 12, 24, and 48 h of culture respectively, and cultured in an incubator at 37°C and 5% CO 2 . Other steps were the same as those of the model group.

[0090] After adding the same mass of nano-sustained release composition encapsulated in the skin-friendly fabric 3 with a diameter of 3 mm to group 2, 200 μL of Candida albicans prepared with K-SFM culture medium at 1×10 6 CFU / mL was added at 6, 12, 24, and 48 h of culture respectively, and cultured in an incubator at 37°C and 5% CO 2 . Other steps were the same as those of the model group.

[0091] After adding the skin-friendly fabric 4 with a diameter of 3 mm to group 3, 200 μL of Candida albicans prepared with K-SFM culture medium at 1×10 6 CFU / mL was added at 6, 12, 24, and 48 h of culture respectively, and cultured in an incubator at 37°C and 5% CO 2 . Other steps were the same as those of the model group.

[0092] After adding the same mass of vitamin C, vitamin B5, and collagen peptide encapsulated in the skin-friendly fabric 4 with a diameter of 3 mm to group 4, 200 μL of Candida albicans prepared with K-SFM culture medium at 1×10 6 CFU / mL was added at 6, 12, 24, and 48 h of culture respectively, and cultured in an incubator at 37°C and 5% CO 2 . Other steps were the same as those of the model group.

[0093] After adding the skin-friendly fabric 5 with a diameter of 3 mm to group 5, 200 μL of Candida albicans prepared with K-SFM culture medium at 1×10 6 CFU / mL was added at 6, 12, 24, and 48 h of culture respectively, and cultured in an incubator at 37°C and 5% CO 2 . Other steps were the same as those of the model group.

[0094] The blank group does not add fabric samples and Candida albicans.

[0095] 2. Detection of NLRP3 inflammasome expression

[0096] For the detection of NLRP3 inflammasome expression in vaginal epithelial cells by immunofluorescence, cells fixed with 4% paraformaldehyde were taken, repaired with citrate repair solution, then permeabilized solution was added, washed with PBS, blocked with 5% bovine serum albumin at 37 °C, and then NLRP3 inflammasome antibody (diluted 1:100, AG-44B-0008, AdipoGen ® ) was added and incubated overnight at 4 °C. After rewarming, it was washed with PBS, and diluted fluorescent secondary antibody (diluted 1:500, Invitrogen) was added according to the instructions, incubated at 37 °C, DAPI (4’,6-diamidino-2-phenylindole, Invitrogen) was added for staining for 9 min, and after washing with PBS, it was observed and photographed under a fluorescence microscope. 7-8 pictures were selected from each group and fluorescence quantification was performed using Image Pro Plus software, and after fluorescence normalization according to the number of cell nuclei, statistical analysis was performed.

[0097] As an important member of the innate immune system, NLRP3 inflammasome can immediately initiate a protective adaptive immune response to resist pathogens in the early stage of infection. However, if NLRP3 inflammasome is over-activated, on the one hand, the inflammatory cytokines and chemokines induced can not only not fully kill fungi and reduce the damage of vaginal epithelial cells, but instead make the vaginal epithelium in a high-inflammatory state, leading to immunopathological inflammation. For example, Candida albicans in the model group causes pathological inflammation to vaginal epithelial cells, accelerating their lysis, which is likely to cause vaginal mucosal damage and pathological injury to the in vivo vaginal environment.

[0098] As Figure 12 and Figure 13 shown, after co-culturing vaginal epithelial cells with Candida albicans in the model group, the level of NLRP3 inflammasome expressed by vaginal epithelial cells began to increase from 6 h, reached the peak at 12 h and then gradually decreased. By 24 h, vaginal epithelial cells began to lyse, and by 48 h, cell rupture and death could be seen. The fluorescence then weakened, while in the blank group, the cell morphology remained intact throughout and the fluorescence intensity remained relatively stable.

[0099] As Figure 12 shown, at 12 h of culture, the normalized fluorescence intensity values of group 15 were significantly lower than those of the model group, and the normalized fluorescence intensity values of group 1 had no significant difference from those of the blank group. As Figure 13 shown, at 48 h of culture, the normalized fluorescence intensity value of the model group was significantly lower than that of the blank group, indicating that vaginal epithelial cells had lysed, while the normalized fluorescence intensity value of group 1 was still significantly higher than that of the model group, and the relative expression level of group 1 was correspondingly higher than that of groups 2-5.

[0100] This indicates that the skin-friendly fabric 3 provided by the present invention and the sanitary napkin prepared based on the same can not only activate the NLRP3 inflammasome to a certain extent within 12 hours to initiate an immune response against Candida albicans, but also prevent the lysis of vaginal epithelial cells due to the inflammatory response and prevent damage to the vaginal mucosa after 48 hours.

[0101] The above are only the preferred specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed by the present invention should be covered within the protection scope of the present invention.

Claims

1. A method for preparing a skin-friendly fabric that simultaneously and continuously releases vitamin B5, vitamin C and collagen peptides, characterized in that: include: preparing a nano sustained-release composition, wherein the nano sustained-release composition is calcium carbonate particles encapsulating vitamin B5, vitamin C and collagen peptide; Treating pure cotton fabric with glutaraldehyde; The nano sustained-release composition was added at 50 mg / cm 2 The ratio was laid between two layers of pure cotton fabric treated with glutaraldehyde; The removal is carried out by rolling with a rolling mill, with a rolling rate of 60%; and The fabric was soaked in deionized water for 3 hours again, taken out and rolled with a rolling machine with a rolling rate of 60%, and dried at room temperature so that vitamin B5, vitamin C and collagen peptides were deeply combined with the pure cotton fabric to obtain the skin-friendly fabric.

2. The preparation method according to claim 1, characterized in that: The steps of preparing the nano sustained-release composition include: Add sodium polyaspartate to the calcium chloride solution, and after the reaction, dropwise add sodium carbonate solution; After the dropwise addition is completed, the inclusion compound solution is added, ultrasonic treatment is performed, and adsorption treatment is continued under stirring; After the adsorption is completed, the solution is centrifuged, washed with ethanol, centrifuged, and freeze-dried to a constant weight to obtain the nanoparticles; The aqueous solution containing the nanoparticles is prepared and reacted with stearic acid in a wet method at room temperature for at least 48 hours, and then washed with ethanol, centrifuged, and freeze-dried to a constant weight to obtain the nano sustained-release composition.

3. The preparation method according to claim 2, characterized in that: The steps of preparing the nano sustained-release composition specifically include: Take 20 mL of 0.1 M sodium carbonate solution and 20 mL of 0.1 M calcium chloride solution, add 666 mg of sodium polyaspartate to the calcium chloride solution and stir for crosslinking for 30 min, then add the sodium carbonate solution dropwise to the calcium chloride solution with sodium polyaspartate added at a rate of 10 mL / min under stirring; After the dropwise addition, 50 mL of a 30 mg / mL vitamin B5 aqueous solution, or an aqueous solution containing 10 mg / mL vitamin C and 30 mg / mL vitamin B5, or a suspension containing 5 mg / mL flaxseed collagen peptide, 10 mg / mL vitamin C and 30 mg / mL vitamin B5 was added and ultrasonicated for 30 min; Then the adsorption was continued for 24 h at a rotation speed of 1000 rpm; After the reaction, the suspension was centrifuged at 12000 rpm for 30 min, washed with ethanol, centrifuged, and freeze-dried to constant weight to obtain nanoparticles; After mixing an aqueous solution containing 80 wt% of nanoparticles with stearic acid, the mixture was placed at room temperature for 72 hours by a wet method, taken out, centrifuged at 12000 rpm for 30 minutes, washed with ethanol, centrifuged, and freeze-dried to constant weight to obtain a nano sustained-release composition.

4. The preparation method according to claim 3, characterized in that: The amount of stearic acid added is 1.0-1.25% of the mass of the nanoparticles.

5. The preparation method according to claim 1, characterized in that: The method of treating the pure cotton fabric with glutaraldehyde specifically comprises: Take 200 mL of 0.5 wt % glutaraldehyde aqueous solution with a pH of 5, add 4 g of magnesium chloride crystals, stir until it is completely dissolved, and then add 200 cm 2 The high-count gauze was treated in a constant temperature water bath oscillator at 60°C for 1 hour, then taken out and washed with deionized water or soaked for 3 hours, then taken out and rolled with a rolling car, with a rolling rate of 60%. No glutaraldehyde was detected in the residual liquid after washing with deionized water again.

6. A method for preparing a sanitary napkin, characterized in that: The sanitary napkin comprises an inner layer facing the body surface and an outer layer facing the clothing surface that is liquid-proof; wherein the inner layer is sewn from a skin-friendly fabric containing vitamin B5, vitamin C and collagen peptides, and the inner layer and the outer layer are sewn into a whole; the outer layer extends outward to completely cover the inner layer, and extends outward to have two side wings; wherein the dividing portion between the inner layer and the outer layer is sewn from elastic spandex yarns that divide the side wings of the inner layer and the outer layer, and the inner layer is sewn in the middle of the outer layer; the edges of the inner layer and the outer layer are both embedded with elastic spandex yarns; the inner layer comprises multiple layers of the skin-friendly fabric; The preparation method comprises: The preparation method according to any one of claims 1 to 5; and The skin-friendly fabric or a plurality of the skin-friendly fabrics are sewn into the outer layer material as the inner layer material to obtain the sanitary napkin.

7. Use of a skin-friendly fabric in preparing a sanitary napkin, characterized in that: The skin-friendly fabric is prepared by the preparation method according to any one of claims 1 to 5.

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