A microbial culture storage device and a culture storage method

Abstract

The present invention discloses a microbial culture storage device and a culture storage method, belonging to the technical field of microbial culture. The microbial culture storage device includes a box body, and a clamping mechanism for clamping culture dishes is arranged inside the box body. The clamping mechanism includes a mounting plate, a plurality of card slots for placing culture dishes are arranged on the mounting plate, a clamping structure is arranged on the side wall of the card slot, and a transmission structure for driving the clamping structure to act is arranged between the fixing plate in the incubator and the mounting plate; a stirring structure for stirring the culture solution and performing ventilation is arranged inside the shell of the culture dish, and a power structure for driving the stirring structure to rotate is arranged inside the box body. By using the microbial culture storage device and the culture storage method of the present invention, the problems that it is not easy to detect metabolic substances during the process of microbial culture using culture dishes and the culture dishes are not stably fixed can be solved.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial culture, and particularly relates to a microbial culture storage device and a culture storage method. Background Art

[0002] The biosynthesis of microorganisms refers to the process in which microorganisms use simple organic or inorganic substances as raw materials and synthesize complex organic molecules or bioactive substances through a series of enzymatic reactions. Microorganisms convert simple precursor substances into complex products through specific metabolic pathways to obtain synthetic organisms. Microorganisms precisely control the biosynthesis process through various mechanisms such as gene expression regulation and enzyme activity regulation. The biosynthesis of microorganisms has extensive applications in the fields of biotechnology, pharmaceuticals, food industry, etc. Antibiotics, enzyme preparations, vitamins, etc. produced by microorganisms are widely used in the medical field. Organic acids, amino acids, enzyme preparations, etc. produced by microorganisms are used for food processing and preservation. Organic acids, biopolymers, etc. synthesized by microorganisms are used for the production of various chemicals and materials. Microorganisms can be used in environmental governance fields such as wastewater treatment and soil remediation. Biofuels produced by microbial fermentation can replace traditional fossil fuels and reduce environmental pollution.

[0003] The biosynthesis of microorganisms is carried out during the cultivation process of microorganisms, and the cultivation of microorganisms plays a relatively important role in the biosynthesis process. Existing cultivation of microorganisms is generally carried out in petri dishes. Since the petri dish is a simple closed box structure, it is not convenient to detect and analyze the metabolites during the cultivation process.

[0004] The existing patent CN202310904767.6 discloses a microbial incubator integrating liquid replacement and sampling, including a box body. Inside the box body, several triangular culture bottles are sequentially placed. One side of the box body is rotatably connected with a sealed box door. One side of the top of the box body is provided with a material bin. Inside the box body below the material bin, a gun head slide rail assembly is installed. A movable gun head is slidably connected to the middle of the gun head slide rail assembly. Several elastic connecting tubes are respectively connected to the middle side wall of the movable gun head. By adopting the design of the movable gun head in cooperation with the gun head slide rail assembly, the multifunctional combination of injecting culture medium, replacing culture medium, and sampling is realized. Compared with manual operation, the steps are simpler. After the above operations are completed, the steps of cleaning the gun head can be repeated and carried out at any time, which can not only reduce the occurrence of the movable gun head being polluted by the external environment, but also avoid the pollution of the inside of the incubator by the residual liquid inside. Although the above patent can realize the replacement of the culture medium, it is only applicable to culture bottles with an open structure and cannot be applied to petri dishes with a closed structure; and the culture bottles are directly placed in the box body, and the fixing effect on the culture bottles is relatively poor. Therefore, it is very necessary to develop a device suitable for petri dishes and convenient for metabolite detection. Summary of the Invention

[0005] The object of the present invention is to provide a microbial culture storage device and a culture storage method, which solve the problems that it is not easy to detect metabolites during the microbial culture process using a culture dish, and the culture dish is not stably fixed.

[0006] To achieve the above object, the present invention provides a microbial culture storage device, including an incubator. Inside the box body of the incubator, a culture dish for culturing microorganisms is placed. A clamping mechanism for clamping the culture dish is arranged inside the box body. The clamping mechanism includes a mounting plate. A number of card slots for placing the culture dish are arranged on the mounting plate. A clamping structure is arranged on the side wall of the card slot. A transmission structure for driving the clamping structure to act is arranged between the fixed plate and the mounting plate inside the incubator. Inside the shell of the culture dish, a stirring structure for stirring the culture solution and performing air exchange is arranged. A power structure for driving the stirring structure to rotate is arranged inside the box body.

[0007] Preferably, the clamping structure includes an arc-shaped clamping plate. The middle of the clamping plate is connected to a vertically arranged transmission plate through a connecting plate. The mounting plate is provided with a limiting hole for limiting the sliding of the transmission plate. The transmission plate slides along the horizontal line passing through the central axis of the card slot. A connecting hole for the connecting plate to pass through is arranged between the limiting hole and the card slot. The connecting plate slides horizontally along the connecting hole. An avoidance groove communicated with the connecting hole is arranged on the side wall of the card slot. The clamping plate is located in the avoidance groove. A sliding structure for driving the transmission plate to slide is arranged at the bottom of the mounting plate. The sliding structure is connected to the transmission structure.

[0008] Preferably, the sliding structure includes a rotating plate. The rotating shaft at the center of the rotating plate is rotatably connected to the mounting plate. The rotating plate is connected to the rotating shaft through a support rod. An arc-shaped sliding groove inclined outward is arranged on the rotating plate. The bottom end of the transmission plate is provided with a fixed pin. The fixed pin is located in the sliding groove and slides along the sliding groove. The mounting plate is provided with a fixed frame. The fixed frame is located outside the rotating plate. The fixed frame is rotatably connected to the rotating plate. The rotating plate located in the middle of the mounting plate is connected to the transmission structure. The adjacent rotating plates are connected through a transmission component. The transmission component includes a driven gear. The driven gear is rotatably arranged on the mounting plate. The driven gear is located between two rotating plates. Tooth teeth meshing with the driven gear are arranged on the side wall of the rotating plate. The adjacent rotating plates are driven by the driven gear. A through hole for the driven gear to pass through is arranged on the fixed frame.

[0009] Preferably, the transmission structure includes a driving gear. The driving gear is rotatably connected to the mounting plate. A first rack meshing with the driving gear is arranged on the fixed plate. An intermediate gear is arranged on the wheel shaft of the driving gear. The intermediate gear meshes with a transmission gear arranged on the rotating shaft of the rotating plate. The wheel diameter of the intermediate gear is smaller than that of the driving gear. The wheel diameter of the transmission gear is larger than that of the intermediate gear. The diameter of the rotating plate is larger than that of the transmission gear. A guide rail for guiding the sliding of the mounting plate is arranged on the fixed plate. A handle for easily pulling the mounting plate out of the box body is arranged at the end of the mounting plate.

[0010] Preferably, a locking structure is provided between the fixing plate and the mounting plate. The locking structure includes a plurality of locking blocks provided on the mounting plate. The locking blocks are provided at one end of the mounting plate inside the box body. Locking holes are provided on the locking blocks. A locking pin adapted to the locking holes is slidably provided on the fixing plate. A slider is provided at the bottom of the locking pin. An installation groove is provided inside the fixing plate. The slider is located in the installation groove and is slidably connected to the installation groove up and down. A through hole for the locking pin to pass through is provided on the installation groove. A first spring for applying an upward thrust to the locking pin is provided between the bottom of the installation groove and the slider; an inclined transmission hole is provided on the slider. A transmission pin is provided on a push rod on one side of the slider. The transmission pin is located in the transmission hole and is slidably connected to the transmission hole. A sliding hole for the push rod to pass through is provided on the fixing plate. The sliding hole communicates with the installation groove and is vertically provided. A push plate is provided at the end of the push rod. A second spring for applying an outward thrust to the push rod is provided between the push plate and the fixing plate.

[0011] Preferably, the stirring structure includes a stirring component and a ventilation component. The stirring component and the ventilation component are coaxially arranged; the ventilation component includes a central tube. The central tube is located at the inner center of the stirring component and is rotationally connected to the stirring component. An air inlet cavity is provided inside the central tube. The top of the central tube is connected to an external air inlet hose through a rotary joint. A plurality of air inlet manifolds are provided at the bottom of the central tube. Air inlet branch pipes are provided on both sides of the air inlet manifold. The air inlet branch pipes are communicated with the air inlet cavity through the air inlet manifold.

[0012] Preferably, the stirring component includes an outer tube. The outer tube is sleeved outside the central tube. The outer tube is rotationally connected to both the central tube and the housing. A partition is provided inside the outer tube. The partition divides the inner cavity of the outer tube into a closed liquid cavity and an exhaust cavity. Stirring rods are provided at the bottom of the outer tube. Liquid inlet ports for allowing liquid to enter the liquid cavity inside the outer tube are provided on the stirring rods. An exhaust port is provided on the side wall of the outer tube. The exhaust port communicates with the exhaust cavity. The exhaust port is located in the upper part inside the housing for discharging metabolic gases; a fixed cover is provided at the top of the housing. The fixed cover is located outside the outer tube and is rotationally connected to the outer tube in a sealed manner. A baffle for dividing the fixed cover into two closed cavities is provided inside the fixed cover. The upper closed cavity of the fixed cover is communicated with the liquid cavity through a liquid outlet. The lower closed cavity of the fixed cover is communicated with the exhaust cavity through an air outlet. A liquid discharge pipe provided on the upper closed cavity of the fixed cover is connected to an external liquid pump. An exhaust pipe provided on the lower closed cavity of the fixed cover is connected to an external air pump.

[0013] Preferably, the power structure includes a slide plate located above the housing. A lead screw is provided on the box body to drive the slide plate to slide along the length direction of the box body. A motor for driving the lead screw to rotate is provided on the box body. A guide rod for guiding the sliding of the slide plate is provided on the box body. A second rack is provided on the slide plate. A third gear meshing with the second rack is provided outside the top of the central tube. A first gear is provided on the central tube. The first gear meshes with a toothed ring provided on the inner wall of the outer tube through a second gear. The second gear is rotatably arranged on the fixed cover.

[0014] Preferably, a heat insulation layer is provided on the box body. A heat-insulating box door for closing the box body is provided at one end of the box body. A heating element and a lighting element are provided inside the box body. Both the heating element and the lighting element are electrically connected to a controller on the box body. A control panel on the box body is electrically connected to the controller.

[0015] Based on the above-mentioned culture storage method of the microorganism culture storage device, it includes the following steps:

[0016] S1. Open the box door, press the push plate, the second spring is compressed, the push plate drives the push rod to slide inwards, the push rod pushes the slider to slide down along the installation groove through the transmission pin and the transmission hole, the first spring is compressed, the slider drives the locking pin to slide down synchronously, and the locking pin slides out of the locking hole;

[0017] S2. Pull the installation plate outwards, the installation plate slides outwards along the guide rail, the driving gear on the installation plate rotates under the action of the first rack on the fixed plate, the driving gear drives the transmission gear to rotate through the intermediate gear, the transmission gear drives the rotating plate to rotate, the rotating plate drives the adjacent rotating plate to rotate synchronously through the driven gear, the rotating plate drives the transmission plate to slide horizontally along the limiting hole through the chute and the fixing pin, the transmission plate drives the clamping plate to slide towards the side wall of the clamping groove through the connecting plate, and after the installation plate is pulled out of the box body, the clamping plate slides into the avoidance groove;

[0018] S3. Place the housing containing the culture solution and inoculated with microorganisms into the clamping groove, insert the bottom plate into the inside of the clamping groove, connect the top end of the central tube to the air inlet hose through a rotary joint, connect the drain pipe to the liquid pump through a hose, and connect the exhaust pipe to the air pump through a hose;

[0019] S4. Push the installation plate inwards, the driving gear rotates in the reverse direction under the action of the first rack, the clamping plate clamps on the outer wall of the housing, and the bottom end of the clamping plate abuts above the bottom plate; the locking pin slides into the locking hole along the inclined surface of the locking block to lock the installation plate and the fixed plate; the third gear contacts and meshes with the second rack;

[0020] S5. Close the chamber door, set the temperature and light inside the chamber, and conduct the cultivation; Start the motor. The motor drives the slide plate to slide through the lead screw. The slide plate drives the second rack to slide. The second rack drives the central tube to rotate through the third gear. The central tube drives the intake main pipe at its bottom to rotate. The central tube drives the gear ring to rotate through the first gear and the second gear. The gear ring drives the outer tube to rotate. The outer tube drives the stirring rod to rotate to stir the culture solution.

[0021] S6. External gas enters the intake cavity through the rotary joint, then enters the culture solution through the intake main pipe and the intake branch pipe to provide gas for the cultivation of microorganisms; Under the action of the liquid pump, the culture solution enters the liquid cavity through the liquid inlet, enters the upper closed cavity of the fixed cover through the liquid outlet, and is then discharged through the drain pipe for metabolite detection; The waste gas generated by the metabolism of the housing enters the exhaust cavity through the exhaust port under the action of the air pump, enters the lower closed cavity of the fixed cover through the air outlet, and is then discharged through the exhaust pipe.

[0022] The advantages and positive effects of the microbial culture storage device and the culture storage method of the present invention are as follows:

[0023] 1. The present invention is provided with a clamping mechanism inside the chamber. The culture dish is clamped through the clamping structure on the clamping structure, improving the stability of the culture dish. The rotating plates between adjacent clamping structures are connected by driven gears, enabling the rotating plates to rotate synchronously, realizing the synchronous clamping of the culture dish by the clamping plates, and facilitating the clamping operation of the culture dish.

[0024] 2. The present invention is provided with a transmission structure between the mounting plate and the fixed plate. When the mounting plate is pulled out of the chamber, the rotating plate is driven to rotate under the action of the transmission structure, so as to realize the sliding of the clamping plate away from the culture dish; When the mounting plate is slid into the chamber, under the action of the transmission structure and the sliding structure, the mounting plate clamps the culture dish, realizing the clamping and fixing of the culture dish, and the operation is convenient.

[0025] 3. The present invention is provided with a locking structure between the mounting plate and the fixed plate. The locking structure improves the connection stability between the mounting plate and the fixed plate, and positions the mounting plate, improving the position stability of the mounting plate; By pressing the push plate, the push rod drives the slider and the locking pin to slide downward under the action of the transmission hole and the transmission pin, facilitating the separation of the fixed plate from the mounting plate. The locking and separation operations between the mounting plate and the fixed plate are convenient.

[0026] 4. The present invention is provided with a ventilation component, enabling external oxygen or the gas required for the reproduction of microorganisms to enter the intake cavity through the intake hose and the rotary joint, and then enter the culture solution through the intake main pipe and the intake branch pipe, increasing the dissolved amount of gas in the culture solution, and being beneficial to improving the reproduction speed of microorganisms.

[0027] 5. By providing a stirring assembly in the present invention, the culture medium is pumped out by a liquid pump, facilitating the detection of the culture medium. Moreover, the metabolic gases generated by the reproduction of microorganisms can be discharged from the culture dish, reducing the inhibitory effect of the metabolic gases on the reproduction of microorganisms and being conducive to improving the reproduction speed of microorganisms. The stirring rod stirs the culture medium, which is conducive to enhancing the uniformity of the distribution of microorganisms and the distribution of oxygen, etc., and is conducive to increasing the reproduction speed.

[0028] 6. In the present invention, a power structure for driving the rotation of the stirring structure is provided inside the box body. The central tube is driven to rotate by the power structure, and the central tube drives the outer tube to rotate in the opposite direction through the first gear, the second gear, and the toothed ring, providing a stirring effect on the culture medium.

[0029] The technical solution of the present invention will be further described in detail below with reference to the drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 is a three-dimensional structural schematic diagram of an embodiment of the present invention;

[0031] Figure 2 is a front view structural schematic diagram of an embodiment of the present invention;

[0032] Figure 3 is a front three-dimensional structural schematic diagram of a clamping mechanism of an embodiment of the present invention;

[0033] Figure 4 is a back three-dimensional structural schematic diagram of a clamping mechanism of an embodiment of the present invention;

[0034] Figure 5 is a three-dimensional structural schematic diagram of a transmission structure of an embodiment of the present invention;

[0035] Figure 6 is a front view structural schematic diagram of a transmission structure of an embodiment of the present invention;

[0036] Figure 7 is a cross-sectional structural schematic diagram of a clamping structure of an embodiment of the present invention;

[0037] Figure 8 is a schematic diagram of a locking structure of an embodiment of the present invention;

[0038] Figure 9 is a three-dimensional structural schematic diagram of a culture dish of an embodiment of the present invention;

[0039] Figure 10 is a cross-sectional structural schematic diagram of a culture dish of an embodiment of the present invention;

[0040] Figure 11 is an attachment Figure 10 in enlarged view A;

[0041] Figure 12For attachment Figure 10 Enlarged view of B in the figure.

[0042] Reference numerals

[0043] 1. Incubator; 11. Box body; 12. Fixed plate; 13. Guide rail; 14. First rack; 15. Slide plate; 16. Second rack; 17. Lead screw; 18. Guide rod; 19. Motor; 110. Locking pin; 111. Slide block; 112. Installation groove; 113. Transmission hole; 114. Transmission pin; 115. First spring; 116. Second spring; 117. Push rod; 118. Push plate;

[0044] 2. Clamping mechanism; 21. Mounting plate; 22. Card slot; 23. Clamping plate; 24. Connecting plate; 25. Transmission plate; 26. Fixed pin; 27. Limit hole; 28. Avoidance groove; 29. Rotating plate; 210. Chute; 211. Fixed frame; 212. Driving gear; 213. Intermediate gear; 214. Transmission gear; 215. Support rod; 216. Driven gear; 217. Locking block; 218. Locking hole;

[0045] 3. Petri dish; 31. Shell; 32. Bottom plate; 33. Central tube; 34. Outer tube; 35. Total intake pipe; 36. Intake branch pipe; 37. Intake cavity; 38. Stirring rod; 39. Liquid inlet; 310. Liquid cavity; 311. Partition board; 312. Exhaust port; 313. Exhaust cavity; 314. Fixed cover; 315. Baffle; 316. Liquid outlet; 317. Gas outlet; 318. Drain pipe; 319. Exhaust pipe; 320. First gear; 321. Second gear; 322. Tooth ring; 323. Third gear. Detailed implementation manners

[0046] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "upper", "lower", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the product of the present invention is usually placed during use. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention. In the description of the present invention, it should also be noted that unless otherwise clearly specified and defined, the terms "set", "installed", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0047] The following will describe in detail the implementation manners of the present invention with reference to the drawings.

[0048] As Figure 1 、 Figure 2 shown. A microbial culture storage device includes an incubator 1. The incubator 1 is a rectangular box body 11. An opening for easily placing a culture dish 3 is provided at the front end of the incubator 1, and a box door is hinged at the opening. A heat insulation layer is provided on the box body 11, and the box door is a heat-insulating box door to improve the heat preservation effect of the box body 11. A heating element and a lighting element are provided inside the box body 11. The heating element can be a heating wire, a heating rod or a heating lamp to provide a heat source for the cultivation of microorganisms. The lighting element is a lighting lamp. A temperature sensor and a light intensity sensor are provided inside the box body 11. Both the temperature sensor and the light intensity sensor are electrically connected to the controller on the box body 11 according to existing technologies as needed, and are used to control the temperature and light conditions inside the box body 11. The control panel on the box body 11 is electrically connected to the controller according to existing technologies as needed. The control panel is used to display parameters such as the temperature inside the box body 11 and set the parameters. A storage battery can be set inside the box as needed or directly connected to the mains through a plug. The fixing plate 12 inside the box body 11 can also be set as a heat-insulating plate, so as to divide the box body 11 into several independent culture spaces, and different temperatures and lights are set for different culture spaces through the heating element and the lighting element, so as to study the influence of different culture environments on the cultivation of microorganisms.

[0049] As Figure 3 、 Figure 4 、 Figure 7 shown. A clamping mechanism 2 for clamping the culture dish 3 is provided inside the box body 11. The clamping mechanism 2 includes a mounting plate 21. A number of card slots 22 for placing the culture dish 3 are provided on the mounting plate 21. The diameter of the card slot 22 is slightly larger than the diameter of the bottom plate 32 of the culture dish 3. A clamping structure is provided on the side wall of the card slot 22. The clamping structure includes an arc-shaped clamping plate 23 for clamping on the outer wall of the housing 31. The middle part of the clamping plate 23 is fixedly connected to a vertically arranged transmission plate 25 through a connecting plate 24. A limiting hole 27 for limiting the sliding of the transmission plate 25 is provided on the mounting plate 21. The width of the limiting hole 27 is slightly larger than the width of the transmission plate 25, so that the transmission plate 25 slides along the horizontal line passing through the central axis of the card slot 22, and cannot slide and rotate in other directions, ensuring that the clamping plate 23 slides along the horizontal line passing through the central axis of the card slot 22. A connecting hole for the connecting plate 24 to pass through is provided between the limiting hole 27 and the card slot 22. The connecting plate 24 slides horizontally along the connecting hole. An avoidance groove 28 communicating with the connecting hole is provided on the side wall of the card slot 22. The clamping plate is located in the avoidance groove 28 to prevent the clamping plate from affecting the placement of the culture dish 3 into the card slot 22.

[0050] As Figure 5 、 Figure 6As shown in the figure. A sliding structure for driving the sliding of the transmission plate 25 is provided at the bottom of the mounting plate 21, and the sliding structure is connected to the transmission structure. The sliding structure includes a rotating plate 29. The rotating shaft at the center of the rotating plate 29 is rotatably connected to the mounting plate 21 through a bearing. The rotating plate 29 is fixedly connected to the rotating shaft through a support rod 215. An arc-shaped chute 210 that slopes outward is provided on the rotating plate 29. A fixing pin 26 is fixedly provided at the bottom end of the transmission plate 25, and the fixing pin 26 is located in the chute 210 and slides along the chute 210. The rotating plate 29 drives the transmission plate 25 to slide horizontally through the chute 210 and the fixing pin 26. A fixing frame 211 is fixedly provided on the mounting plate 21. The fixing frame 211 is located outside the rotating plate 29, and the fixing frame 211 is rotatably connected to the rotating plate 29 through a bearing. The rotating plate 29 located in the middle of the mounting plate 21 is connected to the transmission structure.

[0051] Adjacent rotating plates 29 are connected through a transmission component. The transmission component includes a driven gear 216. The driven gear 216 is rotatably arranged on the mounting plate 21 through a bearing. The driven gear 216 is located between two rotating plates 29. Teeth meshing with the driven gear 216 are provided on the side wall of the rotating plate 29. Adjacent rotating plates 29 are driven through the driven gear 216. A through hole for the driven gear 216 to pass through is provided on the fixing frame 211. The rotating plates 29 are meshed through the driven gear 216, so that the rotating plates 29 rotate synchronously, and the clamping plates 23 clamp the culture dish 3 synchronously.

[0052] A transmission structure for driving the clamping structure to act is provided between the fixing plate 12 in the incubator 1 and the mounting plate 21. The transmission structure includes a driving gear 212. The driving gear 212 is rotatably connected to the mounting plate 21 through a bearing. A first rack 14 meshing with the driving gear 212 is fixedly provided on the fixing plate 12. The first rack 14 is arranged along the sliding direction of the mounting plate 21. An intermediate gear 213 is fixedly provided on the axle of the driving gear 212. The intermediate gear 213 meshes with a transmission gear 214 fixedly provided on the rotating shaft of the rotating plate 29. The diameter of the intermediate gear 213 is smaller than the diameter of the driving gear 212, the diameter of the transmission gear 214 is larger than the diameter of the intermediate gear 213, and the diameter of the rotating plate 29 is larger than the diameter of the transmission gear 214, realizing the decelerated rotation of the rotating plate 29. A guide rail 13 for guiding the sliding of the mounting plate 21 is fixedly provided on the fixing plate 12. A handle for conveniently pulling the mounting plate 21 out of the box body 11 is provided at the end of the mounting plate 21.

[0053] When the mounting plate 21 is pulled out of the box body 11, the rotating plate 29 is driven to rotate under the action of the transmission structure, so as to realize the sliding of the clamping plate 23 in a direction away from the culture dish 3; when the mounting plate 21 is slid into the box body 11, under the action of the transmission structure and the sliding structure, the mounting plate 21 clamps the culture dish 3, realizing the clamping and fixing of the culture dish 3, and the operation is convenient.

[0054] As shown in Figure 8 Figure. A locking structure is provided between the fixing plate 12 and the mounting plate 21. The locking structure includes a plurality of locking blocks 217 provided on the mounting plate 21. The locking blocks 217 are fixedly arranged at one end of the mounting plate 21 inside the box body 11. A locking hole 218 is provided on the locking block 217, and a locking pin 110 adapted to the locking hole 218 is slidably arranged on the fixing plate 12. An inclined surface facilitating the sliding of the locking pin 110 into the locking hole 218 is provided on the locking block 217. A slider 111 is fixedly arranged at the bottom of the locking pin 110. An installation groove 112 is provided inside the fixing plate 12. The slider 111 is located in the installation groove 112 and is slidably connected to the installation groove 112 up and down. A through hole through which the locking pin 110 passes is provided on the installation groove 112. The locking pin 110 is slidably connected to the through hole, and the locking pin 110 can only slide up and down under the action of the through hole, and cannot perform horizontal sliding and rotation. A first spring 115 applying an upward thrust to the locking pin 110 is provided between the bottom of the installation groove 112 and the slider 111.

[0055] An inclined transmission hole 113 is provided on the slider 111. A transmission pin 114 is fixedly arranged on a push rod 117 on one side of the slider 111. The transmission pin 114 is located in the transmission hole 113 and is slidably connected to the transmission hole 113. The push rod 117 and the locking pin 110 are arranged in one-to-one correspondence. A sliding hole through which the push rod 117 passes is provided on the fixing plate 12. The sliding hole is communicated with the installation groove 112 and is vertically arranged. A push plate 118 is fixedly arranged at the end of the push rod 117. The push plate 118 fixedly connects the push rods 117 together. A second spring 116 applying an outward thrust to the push rod 117 is provided between the push plate 118 and the fixing plate 12.

[0056] The locking structure improves the connection stability between the mounting plate 21 and the fixing plate 12, and positions the mounting plate 21 to improve the position stability of the mounting plate 21; by pressing the push plate 118, the push rod 117 drives the slider 111 and the locking pin 110 to slide downward under the action of the transmission hole 113 and the transmission pin 114, facilitating the separation of the fixing plate 12 from the mounting plate 21. The locking and separation operations between the mounting plate 21 and the fixing plate 12 are convenient.

[0057] As shown in Figure 9 , Figure 10 , Figure 11 , Figure 12As shown in the figure. Inside the housing 31 of the culture dish 3, there is a stirring structure for stirring the culture medium and ventilating. The stirring structure includes a stirring component and a ventilation component, and the stirring component and the ventilation component are coaxially arranged. The ventilation component includes a central tube 33, which is located at the inner center of the stirring component and is rotatably connected to the stirring component through a bearing. An air inlet cavity 37 is arranged inside the central tube 33, and the top end of the central tube 33 is connected to an external air inlet hose through a rotary joint. A plurality of air inlet main pipes 35 are arranged at the bottom end of the central tube 33, and a plurality of air inlet branch pipes 36 are arranged on both sides of the air inlet main pipe 35. The air inlet branch pipes 36 are communicated with the air inlet cavity 37 through the air inlet main pipe 35.

[0058] The stirring component includes an outer tube 34, which is sleeved outside the central tube 33. The outer tube 34 is rotatably connected to both the central tube 33 and the housing 31 through bearings. A partition 311 is arranged inside the outer tube 34, and the partition 311 divides the inner cavity of the outer tube 34 into a closed liquid cavity 310 and an exhaust cavity 313. A stirring rod 38 is arranged at the bottom of the outer tube 34, and a liquid inlet 39 for allowing liquid to enter the liquid cavity 310 inside the outer tube 34 is arranged on the stirring rod 38. The liquid inlet 39 is located below the liquid level of the culture medium. An exhaust port 312 is arranged on the side wall of the outer tube 34, and the exhaust port 312 is communicated with the exhaust cavity 313. The exhaust port 312 is located in the upper part inside the housing 31 and above the culture medium, and is used to discharge metabolic gases, reducing the inhibitory effect of metabolic gases on the reproduction of microorganisms.

[0059] A fixing cover 314 is arranged at the top of the housing 31. The fixing cover 314 is located outside the outer tube 34 and is rotatably and sealedly connected to the outer tube 34 through a bearing and a sealing ring. A baffle 315 that divides the fixing cover 314 into two closed cavities is fixedly arranged inside the fixing cover 314. The upper closed cavity of the fixing cover 314 is communicated with the liquid cavity 310 through a liquid outlet 316, and a drain pipe 318 fixedly arranged on the upper closed cavity of the fixing cover 314 is connected to an external liquid pump. The lower closed cavity of the fixing cover 314 is communicated with the exhaust cavity 313 through an air outlet 317, and an exhaust pipe 319 fixedly arranged on the lower closed cavity of the fixing cover 314 is connected to an external air pump.

[0060] Solenoid valves are arranged on the air inlet branch pipes 36, the liquid inlets 39, and the exhaust ports 312 as required. The solenoid valves are electrically connected to a controller using existing technology and are used to control the opening or closing of the pipelines. A corresponding gas concentration sensor can be arranged inside the housing 31 as required, which is used to detect the oxygen or metabolic gas concentration inside the housing 31, so as to automatically supplement oxygen or discharge metabolic gases into the housing 31.

[0061] By setting up the air exchange component, oxygen from the outside or gases required for the reproduction of microorganisms enter the intake cavity 37 through the intake hose and the rotary joint, and then enter the culture solution through the intake main pipe 35 and the intake branch pipe 36, increasing the dissolved amount of gases in the culture solution and facilitating the improvement of the reproduction speed of microorganisms.

[0062] By setting up the stirring component, the culture solution is pumped out by the liquid pump, facilitating the detection of the culture solution; and the metabolic gases generated by the reproduction of microorganisms can be discharged from the culture dish 3, reducing the inhibitory effect of the metabolic gases on the reproduction of microorganisms and facilitating the improvement of the reproduction speed of microorganisms. The stirring rod 38 stirs the culture solution, facilitating the improvement of the uniformity of the distribution of microorganisms and the uniformity of the distribution of oxygen, etc., and facilitating the improvement of the reproduction speed.

[0063] Both the liquid pump and the air pump are electrically connected to the controller according to needs using existing technologies.

[0064] Inside the box body 11, there is a power structure that drives the stirring structure to rotate. The power structure includes a sliding plate 15, and the sliding plate 15 is located above the housing 31. On the box body 11, there is a lead screw 17 that drives the sliding plate 15 to slide along the length direction of the box body 11. The lead screw 17 is rotatably connected to the box body 11 through a bearing, and the lead screw 17 is threadedly connected to the sliding plate 15. Outside the box body 11, there is a motor 19 that drives the lead screw 17 to rotate. The motor 19 is electrically connected to the controller according to needs using existing technologies. On the box body 11, there is a guide rod 18 that guides the sliding of the sliding plate 15.

[0065] On the sliding plate 15, there is a second rack 16 fixedly arranged. Outside the top of the central tube 33, there is a third gear 323 fixedly arranged that meshes with the second rack 16. On the central tube 33, there is a first gear 320 fixedly arranged. The first gear 320 meshes with a gear ring 322 fixedly arranged on the inner wall of the outer tube 34 through a second gear 321. The second gear 321 is rotatably arranged on the fixed cover 314 through a bearing. The central tube 33 drives the outer tube 34 to rotate in the opposite direction through the first gear 320, the second gear 321, and the gear ring 322, providing a stirring effect on the culture solution.

[0066] Based on the above-mentioned culture storage method of the microorganism culture storage device, it includes the following steps:

[0067] S1. Open the box door, press the push plate 118, the second spring 116 is compressed, the push plate 118 drives the push rod 117 to slide inward, the push rod 117 pushes the slider 111 to slide downward along the installation groove 112 through the transmission pin 114 and the transmission hole 113, the first spring 115 is compressed, and the slider 111 drives the locking pin 110 to slide downward synchronously. The locking pin 110 slides out of the locking hole 218.

[0068] S2. Pull the mounting plate 21 outward. The mounting plate 21 slides outward along the guide rail 13. The driving gear 212 on the mounting plate 21 rotates under the action of the first rack 14 of the fixed plate 12. The driving gear 212 drives the transmission gear 214 to rotate through the intermediate gear 213. The transmission gear 214 drives the rotating plate 29 to rotate. The rotating plate 29 drives the adjacent rotating plate 29 to rotate synchronously through the driven gear 216. The rotating plate 29 drives the transmission plate 25 to slide horizontally along the limiting hole 27 through the sliding groove 210 and the fixing pin 26. The transmission plate 25 drives the clamping plate 23 to slide towards the side wall of the clamping groove 22 through the connecting plate 24. After the mounting plate 21 is pulled out of the box body 11, the clamping plate 23 slides into the avoidance groove 28.

[0069] S3. Place the housing 31 containing the culture medium and inoculated with microorganisms into the clamping groove 22, and insert the bottom plate 32 into the inside of the clamping groove 22. Connect the top end of the central tube 33 to the intake hose through a rotary joint, connect the drain pipe 318 to the liquid pump through a hose, and connect the exhaust pipe 319 to the air pump through a hose. The hose meets the length change requirements when the mounting plate 21 slides.

[0070] S4. Push the mounting plate 21 inward. The driving gear 212 rotates reversely under the action of the first rack 14. The clamping plate 23 clamps on the outer wall of the housing 31, and the bottom end of the clamping plate 23 abuts above the bottom plate 32. The locking pin 110 slides into the locking hole 218 along the inclined surface of the locking block 217 to lock the mounting plate 21 and the fixed plate 12. The third gear 323 contacts and meshes with the second rack 16.

[0071] S5. Close the box door, set the temperature and light in the box body 11, and start the cultivation. Start the motor 19. The motor 19 drives the sliding plate 15 to slide through the lead screw 17. The sliding plate 15 drives the second rack 16 to slide. The second rack 16 drives the central tube 33 to rotate through the third gear 323. The central tube 33 drives the intake main pipe 35 at its bottom to rotate. The central tube 33 drives the ring gear 322 to rotate through the first gear 320 and the second gear 321. The ring gear 322 drives the outer tube 34 to rotate. The outer tube 34 drives the stirring rod 38 to rotate to stir the culture medium.

[0072] S6. The external gas enters the intake cavity 37 through the rotary joint, and then enters the culture medium through the intake main pipe 35 and the intake branch pipe 36 to provide gas for the cultivation of microorganisms. Under the action of the liquid pump, the culture medium enters the liquid cavity 310 through the liquid inlet 39, enters the upper closed cavity of the fixed cover 314 through the liquid outlet 316, and is then discharged through the drain pipe 318 for metabolite detection. The waste gas generated by the metabolism of the housing 31 enters the exhaust cavity 313 through the exhaust port 312 under the action of the air pump, enters the lower closed cavity of the fixed cover 314 through the air outlet 317, and is then discharged through the exhaust pipe 319.

[0073] Therefore, by using the microbial culture storage device and the culture storage method of the present invention, it is possible to solve the problems that it is not easy to detect metabolic substances during the microbial culture process using petri dishes, and the petri dishes are not stably fixed.

[0074] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that they can still modify or equivalently replace the technical solutions of the present invention, and these modifications or equivalent replacements cannot make the modified technical solutions deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A microbial culture storage device, including an incubator, wherein a culture dish for culturing microorganisms is placed inside the box body of the incubator, and it is characterized in that: Inside the box body, there is a clamping mechanism for clamping the culture dish. The clamping mechanism includes a mounting plate. A number of slots for placing the culture dish are provided on the mounting plate. A clamping structure is provided on the side wall of the slot. A transmission structure for driving the clamping structure to act is provided between the fixed plate in the incubator and the mounting plate. Inside the shell of the culture dish, there is a stirring structure for stirring the culture solution and performing air exchange. A power structure for driving the stirring structure to rotate is provided inside the box body. The clamping structure includes an arc-shaped clamping plate. The middle of the clamping plate is connected to a vertically arranged transmission plate through a connecting plate. The mounting plate is provided with a limiting hole for limiting the sliding of the transmission plate. The transmission plate slides along the horizontal line passing through the central axis of the slot. A connecting hole for the connecting plate to pass through is provided between the limiting hole and the slot. The connecting plate slides horizontally along the connecting hole. An avoidance groove communicated with the connecting hole is provided on the side wall of the slot. The clamping plate is located in the avoidance groove. A sliding structure for driving the transmission plate to slide is provided at the bottom of the mounting plate. The sliding structure is connected to the transmission structure. The sliding structure includes a rotating plate. The rotating shaft at the center of the rotating plate is rotatably connected to the mounting plate. The rotating plate is connected to the rotating shaft through a support rod. An arc-shaped chute inclined outward is provided on the rotating plate. A fixed pin is provided at the bottom end of the transmission plate. The fixed pin is located in the chute and slides along the chute. A fixed frame is provided on the mounting plate. The fixed frame is located outside the rotating plate. The fixed frame is rotatably connected to the rotating plate. The rotating plate located in the middle of the mounting plate is connected to the transmission structure. The adjacent rotating plates are connected through a transmission component. The transmission component includes a driven gear. The driven gear is rotatably arranged on the mounting plate. The driven gear is located between two rotating plates. Teeth meshing with the driven gear are provided on the side wall of the rotating plate. The adjacent rotating plates are driven by the driven gear. A through hole for the driven gear to pass through is provided on the fixed frame. A locking structure is provided between the fixed plate and the mounting plate. The locking structure includes a number of locking blocks provided on the mounting plate. The locking blocks are provided at one end of the mounting plate inside the box body. Locking holes are provided on the locking blocks. A locking pin adapted to the locking hole is slidably arranged on the fixed plate. A slider is provided at the bottom of the locking pin. An installation groove is provided inside the fixed plate. The slider is located in the installation groove and is slidably connected to the installation groove up and down. A through hole for the locking pin to pass through is provided on the installation groove. A first spring for applying an upward thrust to the locking pin is provided between the bottom of the installation groove and the slider. An inclined transmission hole is provided on the slider. A transmission pin is provided on a push rod on one side of the slider. The transmission pin is located in the transmission hole and is slidably connected to the transmission hole. A sliding hole for the push rod to pass through is provided on the fixed plate. The sliding hole is communicated with the installation groove and is vertically arranged. A push plate is provided at the end of the push rod. A second spring for applying an outward thrust to the push rod is provided between the push plate and the fixed plate.

2. The microbial culture storage device according to claim 1, characterized in that: The transmission structure includes a driving gear which is rotatably connected to the mounting plate. A first rack meshing with the driving gear is provided on the fixing plate. An intermediate gear is provided on the axle of the driving gear. The intermediate gear meshes with a transmission gear provided on the rotating shaft of the rotating plate. The diameter of the intermediate gear is smaller than that of the driving gear, and the diameter of the transmission gear is larger than that of the intermediate gear. The diameter of the rotating plate is larger than that of the transmission gear. A guide rail for guiding the sliding of the mounting plate is provided on the fixing plate. A handle for easily pulling the mounting plate out of the box body is provided at the end of the mounting plate.

3. A microorganism culture storage device according to claim 2, characterized in that: The stirring structure includes a stirring component and a ventilation component, and the stirring component and the ventilation component are coaxially arranged. The ventilation component includes a central tube which is located at the inner center of the stirring component and is rotatably connected to the stirring component. An air inlet cavity is provided inside the central tube. The top of the central tube is connected to an external air inlet hose through a rotary joint. A plurality of air inlet manifolds are provided at the bottom of the central tube. Air inlet branch pipes are provided on both sides of the air inlet manifold, and the air inlet branch pipes are communicated with the air inlet cavity through the air inlet manifold.

4. The microbial culture storage device according to claim 3, characterized in that: The stirring component includes an outer tube which is sleeved outside the central tube. The outer tube is rotatably connected to both the central tube and the housing. A partition is provided inside the outer tube, and the inner cavity of the outer tube is divided into a closed liquid cavity and an exhaust cavity by the partition. A stirring rod is provided at the bottom of the outer tube, and a liquid inlet for allowing liquid to enter the liquid cavity inside the outer tube is provided on the stirring rod. An exhaust port is provided on the side wall of the outer tube, and the exhaust port is communicated with the exhaust cavity. The exhaust port is located in the upper part inside the housing for discharging metabolic gas. A fixing cover is provided at the top of the housing. The fixing cover is located outside the outer tube and is rotatably connected to the outer tube in a sealed manner. A baffle for dividing the fixing cover into two closed cavities is provided inside the fixing cover. The upper closed cavity of the fixing cover is communicated with the liquid cavity through a liquid outlet. The lower closed cavity of the fixing cover is communicated with the exhaust cavity through an air outlet. A liquid discharge pipe provided on the upper closed cavity of the fixing cover is connected to an external liquid pump. An exhaust pipe provided on the lower closed cavity of the fixing cover is connected to an external air pump.

5. The microbial culture storage device according to claim 4, characterized in that: The power structure includes a sliding plate which is located above the housing. A lead screw for driving the sliding plate to slide along the length direction of the box body is provided on the box body. A motor for driving the lead screw to rotate is provided on the box body. A guide rod for guiding the sliding of the sliding plate is provided on the box body. A second rack is provided on the sliding plate. A third gear meshing with the second rack is provided outside the top of the central tube. A first gear is provided on the central tube. The first gear meshes with a toothed ring provided on the inner wall of the outer tube through a second gear, and the second gear is rotatably arranged on the fixing cover.

6. A microbial culture storage device according to claim 5, characterized in that: The box body is provided with a heat insulation layer. A heat-insulating box door for closing the box body is provided at one end of the box body. A heating element and a lighting element are provided inside the box body. Both the heating element and the lighting element are electrically connected to a controller on the box body. A control panel on the box body is electrically connected to the controller.

7. The culture storage method of a microbial culture storage device according to claim 6, characterized in that, It includes the following steps: S1. Open the box door, press the push plate, the second spring is compressed, the push plate drives the push rod to slide inwards, the push rod pushes the slider to slide downwards along the installation groove through the transmission pin and the transmission hole, the first spring is compressed, the slider drives the locking pin to slide downwards synchronously, and the locking pin slides out of the locking hole; S2. Pull the mounting plate outwards. The mounting plate slides outwards along the guide rail. The driving gear on the mounting plate rotates under the action of the first rack on the fixed plate. The driving gear drives the transmission gear to rotate through the intermediate gear. The transmission gear drives the rotating plate to rotate. The rotating plate drives the adjacent rotating plate to rotate synchronously through the driven gear. The rotating plate drives the transmission plate to slide horizontally along the limiting hole through the chute and the fixed pin. The transmission plate drives the clamping plate to slide towards the side wall of the clamping groove through the connecting plate. After the mounting plate is pulled out of the box body, the clamping plate slides into the avoidance groove. S3. Place the housing containing the culture medium and inoculated with microorganisms into the clamping groove. The bottom plate is inserted into the interior of the clamping groove. Connect the top end of the central pipe to the intake hose through the rotary joint. Connect the drain pipe to the liquid pump through a hose. Connect the exhaust pipe to the air pump through a hose. S4. Push the mounting plate inwards. The driving gear rotates reversely under the action of the first rack. The clamping plate clamps on the outer wall of the housing. The bottom end of the clamping plate abuts above the bottom plate. The locking pin slides into the locking hole along the inclined surface of the locking block to lock the mounting plate and the fixed plate. The third gear comes into contact with and meshes with the second rack. S5. Close the box door, set the temperature and light in the box body, and conduct the cultivation. Start the motor. The motor drives the slide plate to slide through the lead screw. The slide plate drives the second rack to slide. The second rack drives the central pipe to rotate through the third gear. The central pipe drives the intake main pipe at its bottom to rotate. The central pipe drives the ring gear to rotate through the first gear and the second gear. The ring gear drives the outer pipe to rotate. The outer pipe drives the stirring rod to rotate to stir the culture medium. S6. The external gas enters the intake cavity through the rotary joint, and then enters the culture medium through the intake main pipe and the intake branch pipe to provide gas for the cultivation of microorganisms. Under the action of the liquid pump, the culture medium enters the liquid cavity through the liquid inlet, enters the upper closed cavity of the fixed cover through the liquid outlet, and is then discharged through the drain pipe for metabolite detection. The waste gas generated by the metabolism of the housing enters the exhaust cavity through the exhaust port under the action of the air pump, enters the lower closed cavity of the fixed cover through the air outlet, and is then discharged through the exhaust pipe.

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