Microorganism detection analyzer

Abstract

The invention relates to the technical field of microbiological detection, and discloses a microbiological detection analyzer, which comprises a central control seat, filter heads and filter cups, the filter heads are arranged in an array along the length direction of the central control seat, the filter heads are used for placing and fixing a filter membrane, the upper ends of the filter heads are provided with corresponding filter cups or corresponding spray rings, and the spray rings are arranged on the central control seat. According to the device, purified water is sprayed firstly to fully soak a filter membrane, and then suction filtration operation is performed, so that activation of surface characteristics of the filter membrane is ensured, the microorganism interception effect is improved, and the accuracy of a detection result is enhanced; by means of the design of the spraying ring, even in places without ideal conditions, uniform infiltration of the filter membrane can be easily achieved, dependence on external conditions is reduced, the feasibility and efficiency of detection work are improved, applicability is high, and the device has the advantages of being high in practicability and convenient and fast to infiltrate the filter membrane.

Description

Technical Field

[0001] The present invention relates to the technical field of microorganism detection, in particular to a microorganism detection analyzer. Background Art

[0002] Microbial detection analyzers are a type of instrument used to evaluate and analyze the content, type and activity of microorganisms in samples. With the continuous improvement of product quality control requirements in the food, pharmaceutical, cosmetics and other industries, as well as the enhancement of environmental protection awareness, the importance of microbial detection has become increasingly prominent. Microbial limit meters, as an important branch of microbial detection analyzers, are mainly used to detect whether the number of microorganisms in liquid or solid samples meets the prescribed standards, especially in the pharmaceutical, food and beverage, cosmetics and other industries.

[0003] Microbial limit analyzers play a vital role in the medical industry, especially in drug production, medical device disinfection verification, and hospital infection control. Their main function is to ensure that the microbial content in medical products and the environment meets strict safety standards, thereby protecting patient health and medical safety.

[0004] Microbial limit analyzers usually work based on membrane filtration. Microorganisms in liquid or dissolved solid samples are retained on a microporous filter membrane through negative pressure filtration. The filter membrane is then transferred to an appropriate culture medium for cultivation, and the number of microorganisms is calculated based on the colonies formed. This method is not only easy to operate, but also can effectively remove interfering substances to ensure the accuracy of the results.

[0005] In the prior art, the filter membrane needs to be soaked in purified water before use, and then placed on the top of the filter head using tweezers. Since the adhesion of the filter membrane will increase after soaking, it will be tightly attached to the top of the filter head. However, in some rapid on-site detection or resource-limited environments, such as mobile laboratories or field operations, it may not be practical to carry a large amount of purified water to soak the filter membrane. The filter membrane cannot be soaked, resulting in inaccurate microbial detection results. Therefore, it is very necessary to design a microbial detection analyzer that is highly practical and convenient to soak the filter membrane. Summary of the invention

[0006] The object of the present invention is to provide a microorganism detection analyzer to solve the problems raised in the above background technology.

[0007] In order to solve the above technical problems, the present invention provides the following technical solutions: a microbial detection analyzer, comprising a central control seat, a filter head and a filter cup, the filter heads are arranged in an array along the length direction of the central control seat, the filter heads are used to place and fix the filter membrane, the upper end of the filter head is provided with the corresponding filter cup or the corresponding spray ring, the filter cup is used to store the detection sample, the spray ring is used to seal and store pure water, the inner wall of the spray ring is provided with a spray member, the spray members are arranged in a circular array on the inner wall of the spray ring, the spray members are used to spray pure water on the filter membrane, a connecting member is movably installed at the lower end of the spray ring, a driving member is fixedly installed at the lower end of the spray ring, the spray ring is driven by the driving member to rotate in a circle, so as to facilitate the uniform penetration of the pure water into the filter membrane, the outer wall of the filter head is provided with a sleeve ring, the sleeve ring and the connecting member are docked and fixed to provide a fixing platform for the connecting member.

[0008] According to the above technical solution, the spray ring includes an annular body, a liquid inlet pipe, a liquid outlet pipe, a liquid inlet valve and a liquid outlet valve. An annular cavity is opened in the annular body. The annular cavity is arranged as a closed cavity and pure water is stored inside. A pure water residual monitoring device is provided in the annular cavity. A liquid inlet pipe and a liquid outlet pipe are fixedly installed at one end of the annular body. The liquid inlet pipe is located directly above the liquid outlet pipe. The liquid inlet valve and the liquid outlet valve are fixedly installed on the liquid inlet pipe and the liquid outlet pipe.

[0009] According to the above technical solution, the spraying member includes a delivery pipe, a spray head and a delivery pump. The delivery pump is fixedly installed on the inner wall of the spray ring. The spray head is fixedly installed on one end of the delivery pump through the delivery pipe. The spray head is inclined toward the filter head. The other end of the delivery pump extends to the bottom of the annular cavity through the delivery pump.

[0010] According to the above technical solution, the connecting part includes a connecting ring one, a T-shaped slip ring, a connecting ring two, a connecting ring three and a connecting ring four. The connecting ring one is fixedly installed at the lower end of the spray ring and the T-shaped slip ring is fixedly installed at the lower end. The connecting ring four is arranged at the lower end of the spray ring and the outer diameter of the connecting ring four is the same as the outer diameter of the spray ring. The upper end of the connecting ring four is provided with a T-shaped slide groove, and the T-shaped slip ring is slidably installed in the T-shaped slide groove. The connecting ring two is fixedly installed at the lower end of the spray ring and the outer diameter of the connecting ring two is smaller than the outer diameter of the connecting ring one. The connecting ring three is fixedly installed at the upper end of the connecting ring four. The outer diameter of the connecting ring three is the same as the outer diameter of the connecting ring two and the two are slidably connected. The cross-section of the sleeve ring is arranged in a regular polygon. The lower end of the connecting ring four is provided with a docking area arranged corresponding to the sleeve ring, and the upper half of the sleeve ring is fitly connected in the docking area.

[0011] According to the above technical solution, the connecting ring 1 includes an internal thread connecting ring and an external thread connecting ring. The internal thread connecting ring is fixedly installed at the lower end of the spray ring, and the external thread connecting ring is fixedly installed at the upper end of the T-shaped slip ring. The internal thread connecting ring and the external thread connecting ring are threadedly connected.

[0012] According to the above technical solution, the driving part includes a servo motor, a gear and a rack. The servo motor is fixedly mounted on the lower end surface of the spray ring. The servo motor is located between the connecting ring one and the connecting ring two. The gear is fixedly mounted on the output end of the servo motor. The rack is fixedly mounted on the outer wall of the connecting ring three. The gear and the rack are meshingly connected.

[0013] According to the above technical solution, a magnetic ring 1 is embedded and installed on the upper wall of the docking area, and a magnetic ring 2 is embedded and installed on the upper end surface of the sleeve ring, and the magnetic ring 1 and the magnetic ring 2 are attracted and connected to each other.

[0014] According to the above technical solution, the residual quantity monitoring component includes a guide rod, a float plate, a magnet, a reed switch and an alarm. The guide rod is fixedly installed in the annular cavity, the float plate is movably mounted on the guide rod, the magnet is fixedly installed on the float plate, the reed switch is embedded in the bottom of the annular cavity, the alarm is fixedly installed on the outer wall of the spray ring, and the alarm and the reed switch are electrically connected through a wire.

[0015] According to the above technical solution, the filter head includes a pump head, a sealing ring 1, a filter plate, a sealing ring 2 and a sleeve. The sleeve is fixedly installed on the upper end of the central control seat, the pump head is fixedly installed in the sleeve, and the sealing ring 1, the filter plate and the sealing ring 2 are sequentially arranged on the upper end of the pump head.

[0016] According to the above technical solution, the filter cup includes a cup body, a buckle cover, a screw ring, a limit ring and a sealing gasket. The cup body is arranged at the upper end of the second sealing ring and the lower end face is in contact with the second sealing ring. The buckle cover is arranged at the upper end of the cup body. The limit ring is fixedly sleeved on the outside of the cup body and the sealing gasket is fixedly installed on the upper end of the limit ring. The screw ring is movably sleeved on the outside of the cup body and is threadedly connected to the upper end of the filter head.

[0017] Compared with the prior art, the beneficial effects achieved by the present invention are: (1) High detection accuracy: By spraying pure water to fully saturate the filter membrane before performing the filtration operation, the surface characteristics of the filter membrane are activated, the microbial retention effect is improved, and the accuracy of the test results is enhanced; (2) Simplified operation process: The design of the spray ring makes it easy to achieve uniform penetration of the filter membrane even in places where ideal conditions are not met, reducing dependence on external conditions, improving the feasibility and efficiency of detection work, and having high applicability; (3) Resource saving: Compared with the traditional soaking method, the spray ring only needs a small amount of pure water to complete the soaking of the filter membrane, which is particularly suitable for resource-constrained environments such as mobile laboratories or field operations; (4) High portability: The entire system is compact and lightweight, and does not require a large amount of pure water. It ensures the accuracy of the test results and is easy to carry. It is very suitable for use in emergency response vehicles, temporary laboratories in remote areas, and other occasions. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings: Figure 1 is a first stereoscopic schematic diagram of the present invention; Figure 2 is a second stereoscopic schematic diagram of the present invention; Figure 3 is a first partial stereoscopic schematic diagram of the present invention; Figure 4 is a second partial stereoscopic schematic diagram of the present invention; Figure 5 is a third partial stereoscopic schematic diagram of the present invention; Figure 6 is a fourth partial stereoscopic schematic diagram of the present invention; Figure 7 is a fifth partial stereoscopic schematic diagram of the present invention; Figure 8 The present invention Figure 3 The enlarged schematic diagram of point A in the middle; Fig. 9 The present invention Figure 3 The enlarged schematic diagram of point B in the middle; Fig.10 The present invention Figure 3 The enlarged schematic diagram of the center C; Fig.11 The present invention Figure 4 The enlarged schematic diagram of point D in the middle; Fig.12 The present invention Figure 4 The enlarged schematic diagram of point E in the middle; In the figure: 1-central control seat, 2-filter head, 21-pump head, 22-sealing ring 1, 23-filter plate, 24-sealing ring 2, 25-sleeve, 3-filter cup, 31-cup body, 32-buckle cover, 33-screw ring, 34-limiting ring, 35-sealing pad, 4-spray ring, 41-annular body, 411-annular cavity, 42-liquid inlet pipe, 43-liquid outlet pipe, 44-liquid inlet valve, 45-liquid outlet valve, 5-spraying part, 51-delivery pipe, 52-spraying head, 53-delivery pump, 6-connection Parts, 61-connecting ring 1, 611-internal thread connecting ring, 612-external thread connecting ring, 62-T-shaped slip ring, 63-connecting ring 2, 64-connecting ring 3, 65-connecting ring 4, 651-T-shaped slide groove, 652-docking area, 7-driving part, 71-servo motor, 72-gear, 73-rack, 8-sleeve ring, 9-residue monitoring part, 91-guide rod, 92-floating plate, 93-magnet, 94-reed switch, 95-alarm, 10-magnetic ring 1, 11-magnetic ring 2. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] See also Figure 1-12 The present invention provides a technical solution: a microbial detection analyzer, comprising a central control seat 1, a filter head 2 and a filter cup 3, wherein the filter head 2 is arranged in an array along the length direction of the central control seat 1, and the filter head 2 is used to place and fix the filter membrane, and the upper end of the filter head 2 is provided with the corresponding filter cup 3 or the corresponding spray ring 4, and the filter cup 3 is used to store the test sample, and the spray ring 4 is used to seal and store pure water, and the inner wall of the spray ring 4 is provided with a spray member 5, and the spray member 5 is arranged on the inner wall of the spray ring 4 in a circular array, and the spray member 5 is used to spray the pure water on the filter membrane, and a connecting member 6 is movably installed at the lower end of the spray ring 4, and a driving member 7 is fixedly installed at the lower end of the spray ring 4, and the spray ring 4 is driven by the driving member 7 to rotate in a circle, so as to facilitate the uniform penetration of the pure water into the filter membrane, and a sleeve ring 8 is provided on the outer wall of the filter head 2, and the sleeve ring 8 and the connecting member 6 are fixedly connected to provide a fixing platform for the connecting member 6; The present application is composed of a central control seat 1, a filter head 2 and a filter cup 3. Its components are similar to those of a common microbial limit analyzer, ensuring that the basic functions of the microbial detection instrument are realized. The central control seat 1 serves as the control center of the entire device and is responsible for coordinating the operation of each filter head 2. It also provides a stable support platform so that the instrument can operate safely and reliably, supports the simultaneous operation of multiple filter heads 2, and improves the detection efficiency. The number of filter heads 2 can be flexibly set according to demand. In the present application, the number of filter heads 2 is set to three, and they are respectively shown as a state without installing components, a state with a spray ring 4 installed, and a state with a filter cup 3 installed. Each filter head 2 is started or stopped independently, and the filter cup 3 is used to hold the liquid sample to be detected to ensure that the sample is in a sterile state during the entire filtration process; The spray ring 4 is a major additional component, which provides a necessary pretreatment step for the filter membrane, that is, to soak the filter membrane with pure water to remove possible soluble impurities and activate the surface characteristics of the filter membrane. It is mainly used in some on-site rapid detection or resource-limited environments, such as in mobile laboratories or field operations. It may not be realistic to carry a large amount of purified water to soak the filter membrane. The traditional soaking method requires a certain amount of pure water and the operation is relatively complicated, which is impractical under resource-limited conditions. The design of the spray ring 4 allows the use of a smaller volume of pure water to evenly soak the filter membrane, thereby reducing the need for a large amount of pure water. The fine and uniform spraying by the spraying member 5 in the spray ring 4 can ensure that the filter membrane is completely wetted, thereby ensuring the reliability of the test results. At the same time, the design of the spray ring 4 is also suitable for application scenarios with strict space and weight restrictions, such as emergency response vehicles, temporary laboratories in remote areas, etc., which not only simplifies the operation process, but also improves portability and flexibility, so that high-quality microbial detection can be performed even in places without ideal conditions; The microbial detection operation steps of the present application are as follows: Installation and preparation: Installation routine operation steps are to place the filter membrane on the filter head 2 and ensure that it is correctly fixed. Since the filter membrane is not soaked at this time, the position of the filter membrane is easy to adjust. To ensure the accuracy of the filter membrane position, connect the spray ring 4 to the corresponding filter head 2 (pure water is sufficient under conventional laboratory conditions, and the filter cup 3 can be directly selected); Filter membrane soaking: After installing the spray ring 4, turn on the drive part 7 to make the spray ring 4 start to rotate. At the same time, start the spray part 5 to spray pure water onto the filter membrane. Since the inner wall of the spray ring 4 is provided with spray parts 5 distributed in a circular array, it can be ensured that each spray can cover the entire filter membrane surface to achieve an ideal soaking effect. The design of the spray ring 4 allows the use of a smaller volume of pure water to evenly soak the filter membrane, thereby reducing the need for a large amount of water. This process not only simplifies the operation The operation process also improves portability and flexibility. After spraying is completed, carefully check whether the filter membrane has been completely wetted through the opening at the upper end of the spray ring 4. If any unsoaked parts are found, the spraying program can be started again as needed until all areas are fully soaked; Connect the filter cup 3: When it is confirmed that the filter membrane has been fully soaked, remove the spray ring 4 and connect the filter cup 3 to the corresponding filter head 2. After installation, it should be checked to confirm that the installation is complete; Filtration operation: Once the filter cup 3 is connected, the high-performance diaphragm liquid pump built into the central control seat 1 can be started, and the microorganisms in the liquid can be trapped on the filter membrane by negative pressure filtration; Detection completion: After the filtration is completed, carefully remove the filter membrane and transfer it to the configured solid culture medium for further cultivation, observe and record the colony formation, and calculate the number of microorganisms in the test sample based on this; Specifically, the spray ring 4 includes an annular body 41, a liquid inlet pipe 42, a liquid outlet pipe 43, a liquid inlet valve 44 and a liquid outlet valve 45. An annular cavity 411 is provided in the annular body 41. The annular cavity 411 is provided with a closed cavity and pure water is stored therein. A pure water residual monitoring component 9 is provided in the annular cavity 411. The liquid inlet pipe 42 and the liquid outlet pipe 43 are fixedly installed at one end of the annular body 41. The liquid inlet pipe 42 is located directly above the liquid outlet pipe 43. The liquid inlet valve 44 and the liquid outlet valve 45 are fixedly installed on the liquid inlet pipe 42 and the liquid outlet pipe 43 respectively. The annular body 41 is the core structure of the spray ring 4, and an annular cavity 411 is provided inside for storing pure water. The annular cavity 411 is a closed cavity, which ensures that the pure water inside will not leak. A residual monitoring component 9 is provided inside the annular cavity 411, which can monitor the remaining amount of pure water in real time to ensure that there is enough water for spraying operation when needed; Specifically, the spraying member 5 includes a delivery pipe 51, a spray head 52 and a delivery pump 53. The delivery pump 53 is fixedly mounted on the inner wall of the spray ring 4. The spray head 52 is fixedly mounted on one end of the delivery pump 53 through the delivery pipe 51. The spray head 52 is tilted toward the filter head 2. The other end of the delivery pump 53 extends to the bottom of the annular cavity 411 through the delivery pump 53. The delivery pipe 51 connects the delivery pump 53 and the spray head 52, and is responsible for extracting the pure water in the annular cavity 411 from the bottom and delivering it to the spray head 52. The delivery pipe 51 is made of corrosion-resistant and anti-aging materials to adapt to different water quality conditions. The spray head 52 is installed at the end of the delivery pipe 51 and is tilted toward the filter head 2. This tilt angle is designed to ensure that the sprayed pure water can directly cover the surface of the filter membrane to achieve the purpose of uniform penetration. The spray head 52 is provided with a fine nozzle structure to improve the spraying efficiency and effect; Specifically, the connecting member 6 includes a connecting ring 1 61, a T-shaped slip ring 62, a connecting ring 2 63, a connecting ring 3 64 and a connecting ring 4 65. The connecting ring 1 61 is fixedly installed at the lower end of the spraying ring 4 and the T-shaped slip ring 62 is fixedly installed at the lower end. The connecting ring 4 65 is arranged at the lower end of the spraying ring 4 and the outer diameter of the connecting ring 4 65 is the same as the outer diameter of the spraying ring 4. The upper end of the connecting ring 4 65 is provided with a T-shaped slide groove 651, and the T-shaped slip ring 62 is slidably installed in the T-shaped slide groove 651. The second connecting ring 63 is fixedly mounted on the lower end of the spraying ring 4 and the outer diameter of the second connecting ring 63 is smaller than the outer diameter of the first connecting ring 61. The third connecting ring 64 is fixedly mounted on the upper end of the fourth connecting ring 65. The outer diameter of the third connecting ring 64 is the same as the outer diameter of the second connecting ring 63 and the two are slidably connected. The cross section of the sleeve ring 8 is a regular polygon. The lower end of the fourth connecting ring 65 is provided with a docking area 652 corresponding to the sleeve ring 8, and the upper half of the sleeve ring 8 is fitted and connected in the docking area 652. The connecting ring 1 61 is fixedly installed at the lower end of the spraying ring 4 as the upper supporting structure of the whole connecting system. The T-shaped sliding ring 62 is located at the lower end of the connecting ring 1 61 and is designed with a T-shaped cross section to ensure that it can slide smoothly in the T-shaped slide groove 651 of the connecting ring 4 65. This design not only provides good sliding performance with the connecting ring 4 65, but also enhances the stability of the connection to prevent accidental disengagement. The connecting ring 2 63 and the connecting ring 3 64 are correspondingly arranged, and their outer diameters are smaller than the outer diameter of the connecting ring 1 61, providing a placement space for the subsequent driving member 7, ensuring the compactness of the overall structure, and providing additional support for the rotation of the spraying ring 4, increasing the stability of the rotation of the spraying ring 4, and the cross section of the sleeve ring 8 is arranged in a regular polygon, and the lower half is fitted and connected in the docking area 652 of the connecting ring 4 65. This polygonal design increases the stability of the connection and prevents unnecessary displacement or vibration during the rotation process. Specifically, the connecting ring 1 61 includes an internal thread connecting ring 611 and an external thread connecting ring 612, wherein the internal thread connecting ring 611 is fixedly mounted on the lower end of the spray ring 4, and the external thread connecting ring 612 is fixedly mounted on the upper end of the T-shaped slip ring 62, and the internal thread connecting ring 611 and the external thread connecting ring 612 are threadedly connected; The external thread connection ring 612 is fixedly mounted on the upper end of the T-shaped slip ring 62, and is threadedly connected to the internal thread connection ring 611 to ensure a firm connection between the two. This threaded connection not only provides good mechanical strength, but also facilitates the disassembly and assembly between the spray ring 4 and the connection member 6, and facilitates the subsequent regular inspection and maintenance of the internal drive member 7. Specifically, the driving member 7 includes a servo motor 71, a gear 72 and a rack 73. The servo motor 71 is fixedly mounted on the lower end surface of the spray ring 4. The servo motor 71 is located between the connecting ring 1 61 and the connecting ring 2 63. The gear 72 is fixedly mounted on the output end of the servo motor 71. The rack 73 is fixedly mounted on the outer wall of the connecting ring 3 64. The gear 72 and the rack 73 are meshed and connected. When the position of the spray ring 4 needs to be adjusted, the servo motor 71 is started to drive the gear 72 to rotate. Since the gear 72 is meshed with the rack 73, the rotation of the gear 72 is converted into the linear motion of the rack 73. The linear motion of the rack 73 drives the connecting ring 3 64 to slide along the connecting ring 2 63, thereby realizing the uniform rotation of the spray ring 4 and evenly spraying and soaking the filter membrane through the spraying member 5. Specifically, a magnetic ring 10 is embedded and installed on the upper wall of the docking area 652, and a magnetic ring 11 is embedded and installed on the upper end surface of the sleeve ring 8, and the magnetic ring 10 and the magnetic ring 11 are attracted and connected to each other; The attraction between the magnetic ring 10 and the magnetic ring 2 11 can effectively prevent the spray ring 4 from being displaced or offset during the rotation process, ensuring the uniformity and consistency of the spraying process, while facilitating disassembly. The magnetic connection also has a certain buffering effect, which can absorb mechanical vibration to a certain extent and reduce noise and wear. Specifically, the residual monitoring component 9 includes a guide rod 91, a floating plate 92, a magnet 93, a reed switch 94 and an alarm 95. The guide rod 91 is fixedly installed in the annular cavity 411, the floating plate 92 is movably mounted on the guide rod 91, the magnet 93 is fixedly installed on the floating plate 92, the reed switch 94 is embedded in the bottom of the annular cavity 411, and the alarm 95 is fixedly installed on the outer wall of the spray ring 4. The alarm 95 and the reed switch 94 are electrically connected through a wire. When the water level in the annular cavity 411 is within the normal range, the floating plate 92 is at a higher position, and the magnet 93 is away from the reed switch 94. At this time, the reed switch 94 is in a disconnected state, and the alarm 95 does not work. As the spraying operation proceeds, the amount of water in the annular cavity 411 gradually decreases, and the floating plate 92 also drops accordingly. When the water level drops to the preset minimum limit, the magnet 93 is just close to the reed switch 94, triggering its internal contacts to close. After the reed switch 94 is closed, the current is transmitted to the alarm 95 through the wire, causing it to start and sound an alarm. At this time, the user can hear or see an obvious prompt message, reminding them to immediately replenish pure water to avoid the problem of incomplete membrane penetration due to lack of water. After the user replenishes an appropriate amount of pure water, the floating plate 92 rises again, the magnet 93 is away from the reed switch 94, and the latter returns to a disconnected state, the alarm 95 stops working, and the system resumes normal operation; Specifically, the filter head 2 includes a pump head 21, a sealing ring 1 22, a filter plate 23, a sealing ring 24 and a sleeve 25, wherein the sleeve 25 is fixedly mounted on the upper end of the central control seat 1, the pump head 21 is fixedly mounted in the sleeve 25, and the sealing ring 1 22, the filter plate 23 and the sealing ring 24 are sequentially arranged on the upper end of the pump head 21; The sleeve 25 is fixedly mounted on the upper end of the central control seat 1. As the outer shell of the entire filter head 2, the sleeve 25 not only provides physical support for the internal components, but also plays a protective role to prevent external impurities from entering the system. The pump head 21 is fixedly mounted in the sleeve 25 and is one of the core components of the filter head 2. The pump head 21 is responsible for sucking the fluid from the inlet and filtering it through the filter plate 23 before discharging it. The pump head 21, the sealing ring 1 22, the filter plate 23, the sealing ring 24 and the sleeve 25 are all common components in the microbial detector, and will not be described in detail here. Specifically, the filter cup 3 includes a cup body 31, a buckle cover 32, a screwing ring 33, a limit ring 34 and a sealing gasket 35. The cup body 31 is arranged on the upper end of the sealing ring 24 and the lower end surface is in contact with the sealing ring 24. The buckle cover 32 is arranged on the upper end of the cup body 31. The limit ring 34 is fixedly sleeved outside the cup body 31 and the sealing gasket 35 is fixedly installed on the upper end of the limit ring 34. The screwing ring 33 is movably sleeved outside the cup body 31 and is threadedly connected to the upper end of the filter head 2. When installing the filter cup 3, first place the cup body 31 on the upper end of the sealing ring 24 to ensure close contact between the two. The limiting ring 34 is fixedly sleeved onto the outside of the cup body 31, and the cup body 31 is fixed on the filter head 2 by screwing the screw ring 33. The limiting ring 34 limits the screw ring 33, and the sealing gasket 35 plays a role in strengthening the seal, completing the entire installation process.

[0021] Working principle: The present application is composed of a central control seat 1, a filter head 2 and a filter cup 3. Its components are similar to those of a common microbial limit analyzer, ensuring that the basic functions of the microbial detection instrument are realized. The central control seat 1 serves as the control center of the entire device and is responsible for coordinating the operation of each filter head 2. It also provides a stable support platform so that the instrument can operate safely and reliably, supports the simultaneous operation of multiple filter heads 2, and improves the detection efficiency. The number of filter heads 2 can be flexibly set according to demand. In the present application, the number of filter heads 2 is set to three, and they are respectively displayed as a state without installing components, a state with a spray ring 4 installed, and a state with a filter cup 3 installed. Each filter head 2 is started or stopped independently, and the filter cup 3 is used to hold the liquid sample to be detected to ensure that the sample is in a sterile state during the entire filtration process; The spray ring 4 is a major additional component, which provides a necessary pretreatment step for the filter membrane, that is, to soak the filter membrane with pure water to remove possible soluble impurities and activate the surface characteristics of the filter membrane. It is mainly used in some on-site rapid detection or resource-limited environments, such as in mobile laboratories or field operations. It may not be realistic to carry a large amount of purified water to soak the filter membrane. The traditional soaking method requires a certain amount of pure water and the operation is relatively complicated, which is impractical under resource-limited conditions. The design of the spray ring 4 allows the use of a smaller volume of pure water to evenly soak the filter membrane, thereby reducing the need for a large amount of pure water. The fine and uniform spraying by the spraying member 5 in the spray ring 4 can ensure that the filter membrane is completely wetted, thereby ensuring the reliability of the test results. At the same time, the design of the spray ring 4 is also suitable for application scenarios with strict space and weight restrictions, such as emergency response vehicles, temporary laboratories in remote areas, etc., which not only simplifies the operation process, but also improves portability and flexibility, so that high-quality microbial detection can be performed even in places without ideal conditions; The microbial detection operation steps of the present application are as follows: Installation and preparation: Installation routine operation steps are to place the filter membrane on the filter head 2 and ensure that it is correctly fixed. Since the filter membrane is not soaked at this time, the position of the filter membrane is easy to adjust. To ensure the accuracy of the filter membrane position, connect the spray ring 4 to the corresponding filter head 2 (pure water is sufficient under conventional laboratory conditions, and the filter cup 3 can be directly selected); Filter membrane soaking: After installing the spray ring 4, turn on the drive part 7 to make the spray ring 4 start to rotate. At the same time, start the spray part 5 to spray pure water onto the filter membrane. Since the inner wall of the spray ring 4 is provided with spray parts 5 distributed in a circular array, it can be ensured that each spray can cover the entire filter membrane surface to achieve an ideal soaking effect. The design of the spray ring 4 allows the use of a smaller volume of pure water to evenly soak the filter membrane, thereby reducing the need for a large amount of water. This process not only simplifies the operation The operation process also improves portability and flexibility. After spraying is completed, carefully check whether the filter membrane has been completely wetted through the opening at the upper end of the spray ring 4. If any unsoaked parts are found, the spraying program can be started again as needed until all areas are fully soaked; Connect the filter cup 3: When it is confirmed that the filter membrane has been fully soaked, remove the spray ring 4, and connect the filter cup 3 to the corresponding filter head 2. After installation, it should be checked to confirm that the installation is complete; Filtration operation: Once the filter cup 3 is connected, the high-performance diaphragm liquid pump built into the central control seat 1 can be started, and the microorganisms in the liquid are trapped on the filter membrane by negative pressure filtration; Detection completion: After the filtration is completed, carefully remove the filter membrane and transfer it to the configured solid culture medium for continued cultivation, observe and record the colony formation, and calculate the number of microorganisms in the test sample based on this.

[0022] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0023] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A microbial detection analyzer, comprising a central control seat (1), a filter head (2) and a filter cup (3), characterized in that: The filter heads (2) are arranged in an array along the length direction of the central control seat (1); the filter heads (2) are used to place and fix the filter membrane; the upper end of the filter head (2) is provided with a corresponding filter cup (3) or a corresponding spray ring (4); the filter cup (3) is used to store the test sample; the spray ring (4) is used to seal and store pure water; the inner wall of the spray ring (4) is provided with a spray element (5); the spray element (5) is arranged in a circular array on the inner wall of the spray ring (4). The spraying member (5) is used for spraying pure water on the filter membrane. A connecting member (6) is movably mounted on the lower end of the spraying ring (4). A driving member (7) is fixedly mounted on the lower end of the spraying ring (4). The spraying ring (4) is driven by the driving member (7) to rotate in a circle so as to facilitate uniform penetration of the pure water into the filter membrane. A sleeve ring (8) is provided on the outer wall of the filter head (2). The sleeve ring (8) and the connecting member (6) are butt-jointed and fixed to provide a fixing platform for the connecting member (6).

2. A microbial detection analyzer according to claim 1, characterized in that: The spray ring (4) comprises an annular body (41), a liquid inlet pipe (42), a liquid outlet pipe (43), a liquid inlet valve (44) and a liquid outlet valve (45); an annular cavity (411) is provided in the annular body (41); the annular cavity (411) is arranged as a closed cavity and stores pure water; a pure water residual monitoring component (9) is provided in the annular cavity (411); a liquid inlet pipe (42) and a liquid outlet pipe (43) are fixedly mounted on one end of the annular body (41); the liquid inlet pipe (42) is located directly above the liquid outlet pipe (43); and a liquid inlet valve (44) and a liquid outlet valve (45) are fixedly mounted on the liquid inlet pipe (42) and the liquid outlet pipe (43), respectively.

3. A microbial detection analyzer according to claim 2, characterized in that: The spraying member (5) comprises a delivery pipe (51), a spray head (52) and a delivery pump (53); the delivery pump (53) is fixedly mounted on the inner wall of the spray ring (4); one end of the delivery pump (53) is fixedly mounted with the spray head (52) via the delivery pipe (51); the spray head (52) is arranged to be inclined toward the filter head (2); and the other end of the delivery pump (53) extends through the delivery pump (53) to the bottom of the annular cavity (411).

4. A microbial detection analyzer according to claim 2, characterized in that: The connecting member (6) comprises a connecting ring 1 (61), a T-shaped slip ring (62), a connecting ring 2 (63), a connecting ring 3 (64) and a connecting ring 4 (65); the connecting ring 1 (61) is fixedly mounted on the lower end of the spraying ring (4) and the T-shaped slip ring (62) is fixedly mounted on the lower end; the connecting ring 4 (65) is arranged on the lower end of the spraying ring (4) and the outer diameter of the connecting ring 4 (65) is the same as the outer diameter of the spraying ring (4); a T-shaped slide groove (651) is formed on the upper end of the connecting ring 4 (65), and the T-shaped slip ring (62) is slidably mounted in the T-shaped slide groove (651); The second connecting ring (63) is fixedly mounted on the lower end of the spraying ring (4) and the outer diameter of the second connecting ring (63) is smaller than the outer diameter of the first connecting ring (61). The third connecting ring (64) is fixedly mounted on the upper end of the fourth connecting ring (65). The outer diameter of the third connecting ring (64) is the same as the outer diameter of the second connecting ring (63) and the two are slidably connected. The cross section of the sleeve ring (8) is arranged in a regular polygonal shape. The lower end of the fourth connecting ring (65) is provided with a docking area (652) arranged corresponding to the sleeve ring (8), and the upper half of the sleeve ring (8) is fitted and connected in the docking area (652).

5. A microbial detection analyzer according to claim 4, characterized in that: The connecting ring 1 (61) comprises an internally threaded connecting ring (611) and an externally threaded connecting ring (612); the internally threaded connecting ring (611) is fixedly mounted on the lower end of the spraying ring (4); the externally threaded connecting ring (612) is fixedly mounted on the upper end of the T-shaped slip ring (62); the internally threaded connecting ring (611) and the externally threaded connecting ring (612) are threadedly connected.

6. A microbial detection analyzer according to claim 4, characterized in that: The driving member (7) comprises a servo motor (71), a gear (72) and a rack (73); the servo motor (71) is fixedly mounted on the lower end surface of the spray ring (4); the servo motor (71) is located between the first connecting ring (61) and the second connecting ring (63); the gear (72) is fixedly mounted on the output end of the servo motor (71); the rack (73) is fixedly mounted on the outer wall of the third connecting ring (64); and the gear (72) and the rack (73) are meshingly connected.

7. A microbial detection analyzer according to claim 4, characterized in that: A magnetic ring 1 (10) is embedded and installed on the upper wall of the docking area (652), and a magnetic ring 2 (11) is embedded and installed on the upper end surface of the sleeve ring (8); the magnetic ring 1 (10) and the magnetic ring 2 (11) are attracted and connected to each other.

8. A microbial detection analyzer according to claim 2, characterized in that: The residual quantity monitoring component (9) comprises a guide rod (91), a floating plate (92), a magnet (93), a reed switch (94) and an alarm (95); the guide rod (91) is fixedly mounted in the annular cavity (411); the floating plate (92) is movably sleeved on the guide rod (91); the magnet (93) is fixedly mounted on the floating plate (92); the reed switch (94) is embedded in the bottom of the annular cavity (411); the alarm (95) is fixedly mounted on the outer wall of the spray ring (4); and the alarm (95) and the reed switch (94) are electrically connected via a wire.

9. A microbial detection analyzer according to claim 1, characterized in that: The filter head (2) comprises a pump head (21), a sealing ring 1 (22), a filter plate (23), a sealing ring 2 (24) and a sleeve (25); the sleeve (25) is fixedly mounted on the upper end of the central control seat (1); the pump head (21) is fixedly mounted in the sleeve (25); the sealing ring 1 (22), the filter plate (23) and the sealing ring 2 (24) are sequentially arranged on the upper end of the pump head (21).

10. A microbial detection analyzer according to claim 9, characterized in that: The filter cup (3) comprises a cup body (31), a buckle cover (32), a screwing ring (33), a limiting ring (34) and a sealing gasket (35); the cup body (31) is arranged on the upper end of the second sealing ring (24) and the lower end surface is in contact with the second sealing ring (24); the buckle cover (32) is arranged on the upper end of the cup body (31); the limiting ring (34) is fixedly sleeved outside the cup body (31) and the sealing gasket (35) is fixedly installed on the upper end of the limiting ring (34); the screwing ring (33) is movably sleeved outside the cup body (31) and is threadedly connected to the upper end of the filter head (2).

Images