Culture dish equipment for collecting mesenchymal stem cell factors

Abstract

The invention belongs to the technical field of mesenchymal stem cell culture, and discloses a culture dish device for collecting mesenchymal stem cell factors, the culture dish device comprises a culture assembly, one end of the culture assembly is provided with a connecting cavity, the connecting cavity is internally provided with a connecting assembly, one end, far away from the culture assembly, of the connecting assembly is connected with a protective assembly, and the protective assembly is provided with a protective cover. At least one vibration spring is connected between the connecting cavity and the protection assembly. Through mutual cooperation of the arranged protection assembly and the connecting assembly, the position of the vessel can be fixed, so that a user can use the vessel conveniently, discomfort caused by vessel operation is reduced, the rotation angle of the vessel can be increased, the use performance of the user on the vessel is enhanced, and the practicability of the vessel is improved. Meanwhile, through cooperation with the vibration spring, a user is assisted in uniformly mixing the solution in the vessel, the solution is in full contact with cells in the vessel, and the culture efficiency of the culture assembly is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of mesenchymal stem cell culture, in particular to a culture dish device for collecting mesenchymal stem cell factors. Background Art

[0002] A culture dish is a laboratory vessel used for microbial or cell culture. It consists of a flat disc-shaped bottom and a cover, and is generally made of glass or plastic. The materials of culture dishes are basically divided into two categories, mainly plastic and glass. Glass can be used for plant materials, microbial culture, and animal cell adherent culture. Mesenchymal stem cells are a type of pluripotent stem cells that have all the common properties of stem cells, namely self-renewal and multidirectional differentiation capabilities. They are also the most widely used in clinical applications. Combined with hematopoietic stem cells, they can improve the success rate of transplantation and accelerate hematopoietic reconstruction. The cultured cells have the basic characteristics of mesenchymal stem cells, which provides a theoretical basis for the establishment of a mesenchymal stem cell bank and clinical application.

[0003] The patent with application number CN201710351962.5 discloses a device for culturing human gingival mesenchymal stem cells, including a first traceless solid film, a roller, a wheel frame, a screw barrel, a screw, a nut, a slide rail, a first plate support, a pillar, a slider, a limit block, a second plate support, a suction cup, a cover, a second traceless solid film, a through hole, a push plate, a spring, a push rod, a notch and a 96-well ELISA plate. A wheel frame is installed on the left side of the first support plate, and a roller is installed on the wheel frame. The first traceless solid film is wound on the roller. The upper surface and right side surface of the first plate support are smooth structures. The first traceless solid film can be adhered to the upper surface and the right side surface of the first plate support. The advantages of the above technical features are: the upper and lower layers of 96-well ELISA plates are arranged overlappingly.

[0004] At the same time, during the collection and cultivation process, the user is required to hold the culture dish to a certain position for operation according to the operation requirements. At this time, the user is required to hold the operating component in one hand and the culture dish in the other hand for operation. It is very easy to collide, causing the culture dish to fall or the operating component to be contaminated, affecting the cultivation effect of mesenchymal stem cells and increasing the difficulty of operation for the user. Summary of the invention

[0005] The purpose of the present invention is to solve the above problems. The present invention provides a culture dish device for collecting mesenchymal stem cell factors, which has the advantages of simple operation and high stability.

[0006] To achieve the above object, the present invention provides the following technical solution: a culture dish device for collecting mesenchymal stem cell factors, comprising a culture component, one end of the culture component is provided with a connecting cavity, a connecting component is arranged in the connecting cavity, an end of the connecting component away from the culture component is connected with a protective component, and at least one vibration spring is connected between the connecting cavity and the protective component;

[0007] The connection assembly comprises a fixed sleeve, one end of which is fixedly connected in the connection cavity, and one end of the fixed sleeve away from the culture assembly is slidably provided with a movable rod;

[0008] The protection component comprises a suction cup, one side of the suction cup is connected with a connecting sleeve, one end of the connecting sleeve away from the suction cup is connected with a fixing part, and the fixing part is movably connected to the connecting component.

[0009] Preferably, the culture component comprises a vessel, the connecting cavity is located at one end of the vessel away from the opening, and a reinforcing ring is connected to the end of the vessel away from the connecting cavity.

[0010] Preferably, the suction cup, the connecting sleeve and the fixing part are all made of silicone material, and the fixing part is an annular body, wherein the diameter of the inner ring of the fixing part close to the culture component is larger than the diameter of the fixing part away from the culture component.

[0011] Preferably, the connecting assembly further comprises a movable plate, the movable plate is movably connected to the inner ring of the fixing portion, the movable plate abuts against the inner ring of the fixing portion, and the movable plate will not be separated from the fixing portion.

[0012] Preferably, the connecting assembly further comprises a ball joint, a fixed end of the ball joint is connected to one end of the movable rod, and a movable end of the ball joint is connected to the movable plate.

[0013] Preferably, a docking sleeve is connected to the end of the suction cup away from the connecting sleeve, and a docking groove is opened on the inner side of the connecting sleeve. The docking groove is close to the connection position between the connecting sleeve and the suction cup, wherein the docking sleeve is adapted to the docking groove.

[0014] Preferably, a fold is provided at the connection position between the connecting sleeve and the suction cup.

[0015] Preferably, a movable cavity is provided in the fixing sleeve, an elastic reset device is connected to the movable cavity, and the elastic reset device is in contact with the movable rod.

[0016] Preferably, one end of the vibration spring is connected to the cavity wall of the connecting cavity, and the other end of the vibration spring is connected to the connecting sleeve.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1. Through the cooperation between the protective components and the connecting components, the position of the vessel can be fixed, so that it is convenient for users to use the vessel and reduce the discomfort caused by operating the vessel. The rotation angle of the vessel can be increased to enhance the performance of the vessel for users. At the same time, it cooperates with the vibration spring to assist users to evenly mix the solution in the vessel and fully contact the cells inside the vessel, thereby improving the culture efficiency of the culture component.

[0019] 2. Through the cooperation between the fixed sleeve, the movable rod and the movable plate, the height of the vessel can be fixed while the adsorption between the suction cup and the plane can be controlled, thereby improving the convenience of using the culture dish equipment and reducing the possibility of the vessel falling off when the user operates the cells in the vessel.

[0020] 3. Through the protection components, when it is necessary to operate the cells inside the vessel, the suction cup and the connecting sleeve are used to fix the position of the vessel to maintain the position of the vessel unchanged. When the vessel enters the moving or culturing state, the suction cup and the connecting sleeve are used to wrap the vessel, thereby reducing direct damage to the vessel body when the vessel collides or falls, thereby improving the safety of the vessel. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the three-dimensional structure of the overall device of the present invention;

[0022] Figure 2 It is a structural schematic diagram of another angle of the present invention;

[0023] Figure 3 A schematic diagram of the connection structure between the protection component and the connection component of the present invention;

[0024] Figure 4 It is a schematic cross-sectional structure diagram of the overall device of the present invention;

[0025] Figure 5 This is a schematic diagram of the use structure of the protection component and the cultivation component of the present invention;

[0026] Figure 6 for Figure 5 A schematic diagram of the cross-section of the overall structure;

[0027] Figure 7 It is a schematic diagram of the connection structure between the fixing part and the connecting sleeve of the present invention;

[0028] Figure 8 It is a schematic diagram of the connection structure of the docking groove of the present invention;

[0029] Fig. 9 It is a schematic diagram of the position of the docking groove of the present invention;

[0030] Fig.10 It is a schematic diagram of the splicing structure of the overall structure of the present invention.

[0031] Description of the drawings: 1. Culture component; 101. Vessel; 102. Reinforcement ring; 103. Connecting cavity; 2. Connecting component; 201. Fixed sleeve; 202. Movable rod; 203. Ball joint; 204. Movable plate; 3. Protective component; 301. Suction cup; 302. Connecting sleeve; 3021. Docking groove; 303. Docking sleeve; 304. Fixed part; 4. Vibration spring. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] like Figure 1-Figure 3 As shown, a culture dish device for collecting mesenchymal stem cell factors includes a culture component 1, wherein the culture component 1 can carry and cultivate mesenchymal stem cells, and during the cultivation process, the cultivation status of the mesenchymal stem cells can be detected and judged by observing the cell state in the culture component 1. A connecting cavity 103 is provided at one end of the culture component 1. The connection cavity 103 is set so that the connection height of the connecting component 2 is reduced during the connection process, thereby facilitating the use of the culture dish device. A connecting component 2 is provided in the connecting cavity 103, and a protective component 3 is connected to the end of the connecting component 2 away from the culture component 1. The culture component 1 and the protective component 3 are connected in series through the connecting component 2, thereby ensuring that the protective component 3 is connected to the culture component 1 when it is used. During the process, it can be effectively protected, and the protection component 3 is set, on the one hand, it is convenient for the stability of the culture component 1 during use, and on the other hand, it can ensure the safety of the culture component 1 during movement, and at least one vibration spring 4 is connected between the connecting cavity 103 and the protection component 3. When multiple vibration springs 4 are provided, the multiple vibration springs 4 are distributed between the connecting cavity 103 and the protection component 3 in a circular array with the fixed sleeve 201 as the center. In this scheme, there are two vibration springs 4 between the connecting cavity 103 and the protection component 3. When it is necessary to mix the solution in the culture component 1, the culture component 1 can be shaken at this time, so that the vibration spring 4 can reciprocate and retract, so that the solution in the culture component 1 can be mixed evenly.

[0034] like Figure 1As shown, the connecting component 2 includes a fixed sleeve 201, which is used to limit the moving path of the movable rod 202. One end of the fixed sleeve 201 is fixedly connected to the bottom of the connecting cavity 103, so that the height of the fixed sleeve 201 is reduced by utilizing the depth of the connecting cavity 103, ensuring that when the user uses the culture dish component, its use height will not affect the user's operation and comfort during use, and the movable rod 202 is slidably provided at one end of the fixed sleeve 201 away from the culture component 1, wherein the movable rod 202 can be telescopically moved in the fixed sleeve 201, thereby realizing the adjustment of the height of the connecting component 2, thereby realizing the adjustment of the height and function of the protection component 3, wherein the movable rod 202 does not conflict with the fixed portion 304, thereby ensuring that when the protection component 3 is fixed, the culture component 1 can move.

[0035] like Figure 4 As shown, the protection component 3 includes a suction cup 301, and a connecting sleeve 302 is connected to one side of the suction cup 301. The suction cup 301 and the connecting sleeve 302 cooperate with each other to ensure the connection between the suction cup 301 and the connecting sleeve 302 and the smooth area, so as to ensure that the protection component 3 can fix the position of the culture component 1 during the use of the culture component 1, reduce the number of times the user manually holds the culture component 1, and improve the user's convenience of operation. The end of the connecting sleeve 302 away from the suction cup 301 is connected to a fixing portion 304, and the fixing portion 304 strengthens the connection between the connecting sleeve 302 and the connecting component 2 The connection stability is ensured to ensure that the connecting sleeve 302 will not break during the use of the protection component 3. The fixing portion 304 is movably connected to the connecting component 2. After the protection component 3 fixes the position of the culture dish device, the movably connected between the fixing portion 304 and the connecting component 2 ensures that the vessel 101 can be angularly deflected. When the user operates the vessel 101, the angle of the vessel 101 can be adjusted according to different operating methods. After the protection component 3 fixes the position of the vessel 101, the operating convenience of the culture dish device is improved by changing the angle of the vessel 101.

[0036] During use, the protection component 3 can not only ensure the stability of the position by adsorption with the ground, but also can be turned over so that the suction cup 301 wraps the position of the container 101 in the opposite direction, and the connecting sleeve 302 covers the bottom of the container 101, thereby improving the safety of the container 101 during use and reducing the possibility of damage to the container 101 due to collision.

[0037] It should be noted that the smooth area where the suction cup 301 and the connecting sleeve 302 are connected includes but is not limited to a clean desktop, glass, or other flat surface that can be adsorbed by the protective component 3 .

[0038] Furthermore, the culture component 1 includes a vessel 101, which supports the mesenchymal stem cells. The connecting cavity 103 is located at one end of the vessel 101 away from the opening, and a reinforcement ring 102 is connected to the end of the vessel 101 away from the connecting cavity 103. The opening direction of the vessel 101 is reinforced by the reinforcement ring 102, thereby reducing the possibility of debris falling into the vessel 101 during the friction of the culture component 1 and affecting the cultivation of cells. At the same time, the setting of the connecting cavity 103 will not affect the interior of the vessel 101, thereby ensuring that the vessel 101 can meet the cultivation requirements of mesenchymal stem cells.

[0039] It should be noted that when the vessel 101 is flipped to the maximum angle, the solution of standard depth inside the vessel 101 will not spill, that is, the wall height of the vessel 101 and the elastic change of the vibration spring 4 correspond to each other, ensuring that during the process of vibrating the vessel 101 using the vibration spring 4, the vessel 101 will not leak the solution inside it.

[0040] like Figure 4 As shown, further, in order to ensure that the protection component 3 can be used normally and also to ensure the service life of the protection component 3, the suction cup 301, the connecting sleeve 302 and the fixing portion 304 are all made of silicone material, thereby ensuring that the protection component 3 can be effectively connected to the smooth plane, and the fixing portion 304 is an annular body, ensuring the connection and fixation between the fixing portion 304 and the connecting component 2, and at the same time, by setting the fixing portion 304 into a ring shape, when it is necessary to cancel the position fixation of the protection component 3, the suction cup 301 and the connecting sleeve 302 are inflated through the inner ring of the fixing portion 304, thereby reducing the adsorption force between the protection component 3 and the smooth surface, so as to contact the fixing of the protection component 3, and realize Now, the culture component 1 is moved, wherein the diameter of the inner ring of the fixing part 304 close to the culture component 1 is larger than the diameter of the fixing part 304 away from the culture component 1, thereby ensuring that when the protection component 3 is adsorbed and fixed, the movable plate 204 can seal the inner ring of the fixing part 304, ensuring that a sealed cavity can be formed when the suction cup 301, the connecting sleeve 302 and the fixing part 304 are in contact with the smooth plane, thereby achieving the position fixation of the protection component 3. When the protection component 3 needs to cancel the restriction on the smooth plane, the movable plate 204 is moved so that the external gas can enter the protection component 3 through the inner ring of the fixing part 304, so as to cancel the position fixation of the culture dish equipment by the protection component 3.

[0041] like Figure 6As shown, further, in order to quickly realize the fixing and canceling of the protection component 3, the connecting component 2 also includes a movable plate 204, wherein the movable plate 204 is a colloid material, and the movable plate 204 is movably connected to the inner ring of the fixed part 304, that is, the movable plate 204 can slide in the inner ring of the fixed part 304, and at the same time, the movable plate 204 can be clamped on the inner ring of the fixed part 304, so that the inner ring of the fixed part 304 forms a sealing state, and the movable plate 204 is in conflict with the inner ring of the fixed part 304, and the movable plate 204 will not be separated from the fixed part 304, and then the closing and opening of the inner ring of the fixed part 304 are realized by the movement of the movable plate 204.

[0042] like Figure 6 As shown, further, in order to facilitate the adjustment of the angle of the culture component 1 after the position of the protection component 3 is fixed, and when necessary, the angle of the vessel 101 can also be driven to be adjusted according to the elastic change of the vibration spring 4, the connection component 2 further includes a ball joint 203, and the movable rod 202 and the protection component 3 are rotatably connected through the connection of the ball joint 203. The fixed end of the ball joint 203 is connected to one end of the movable rod 202, and the movable end of the ball joint 203 is connected to the movable plate 204. At the same time, the friction force of the ball joint 203 during rotation can be adjusted. Therefore, the friction force of the ball joint 203 is adjusted according to the use environment of the vessel 101 to ensure that the culture component 1 can meet the use requirements. That is, when the vessel 101 is in the process of culturing and injecting liquid, the friction force of the ball joint 203 is relatively large, so that the vessel 101 will not rotate during use. When the cells in the vessel 101 need to be mixed, the friction force of the ball joint 203 is adjusted to be relatively small, so that when the vibration spring 4 expands and contracts, it can effectively drive the vessel 101 to shake, ensuring that the solution is in full contact with the mesenchymal stem cells.

[0043] The friction force adjustment method of the ball joint 203 is preferably knob control, and the friction force of the ball joint 203 is changed by controlling the change of the resistance force between the knob and the ball joint 203, so as to improve the adjustment efficiency of the friction force of the ball joint 203, thereby meeting the use requirements of the culture dish equipment. The adjustment method is the existing technology and the process will not be described in detail here.

[0044] like Figure 8-Figure 9As shown, further, in order to facilitate the stacking of multiple culture dish devices during storage, reduce space occupancy and improve stacking stability, a docking sleeve 303 is connected to the end of the suction cup 301 away from the connecting sleeve 302, and a docking groove 3021 is opened on the inner side of the connecting sleeve 302, and the docking groove 3021 is close to the connection position between the connecting sleeve 302 and the suction cup 301, wherein the docking sleeve 303 is adapted to the docking groove 3021, and then when the suction cup 301 covers the edge of the vessel 101, when multiple culture dish devices need to be stacked for culture, the docking groove 3021 on one culture dish device can correspond to the docking sleeve 303 on another culture dish device, so that the docking sleeve 303 is embedded in the docking groove 3021, and so on, thereby ensuring that the multiple culture dish devices are docked with each other, and when the culture dish devices are stacked, the bottom thereof is the connecting sleeve 302, and thus has an anti-slip effect when in contact with a plane.

[0045] like Figure 5-Figure 7 As shown, further, in order to facilitate the suction cup 301 to wrap the circumferential wall of the vessel 101, a fold is provided at the connection position between the connecting sleeve 302 and the suction cup 301, and the fold will not interfere with the adsorption of the protective component 3. At the same time, when the suction cup 301 and the connecting sleeve 302 are folded, the fold can prompt the connecting sleeve 302 to be folded to the bottom edge of the vessel 101, and the suction cup 301 wraps the edge of the vessel 101, thereby improving the folding efficiency.

[0046] like Figure 6 As shown, further, in order to facilitate the state change adjustment of the protection component 3, a movable cavity is opened in the fixed sleeve 201, and an elastic reset device is connected in the movable cavity, and the elastic reset device is in contact with the movable rod 202. One end of the vibration spring 4 is connected to the cavity wall of the connecting cavity 103, and the other end of the vibration spring 4 is connected to the connecting sleeve 302 to ensure that when the connecting sleeve 302 and the suction cup 301 cover the vessel 101, the movable rod 202 can be retracted into the fixed sleeve 201 without interfering with the connection position of the connecting sleeve 302. At the same time, when the protection component 3 is restored, when the suction cup 301 is about to be separated from the vessel 101, the elastic reset device can be used to reset the connecting sleeve 302.

[0047] During operation, the culture dish device is first moved to the target area. At this time, the friction force of the ball joint 203 is relatively large to maintain the angle between the connecting component 2 and the protective component 3 without changing, thereby ensuring the stable movement of the culture component 1. When the culture dish device is in the target position, the protective component 3 is brought into contact with and pressed against the target area, so that the suction cup 301 and the connecting sleeve 302 are fully in contact with and adsorbed on the target area. In this process, the movable plate 204 blocks the inner ring of the fixed portion 304, thereby fixing the position of the culture component 1, thereby facilitating the operator to operate and process the substances in the vessel 101.

[0048] When the solution inside the vessel 101 needs to be adsorbed and cleaned, the friction force of the ball joint 203 is maintained unchanged. The angle of the vessel 101 is adjusted and fixed by the user to adjust the angle of the vessel 101, so that the solution in the vessel 101 is gathered to one side, thereby reducing the time the user holds the vessel 101, improving the user's operability, and facilitating the absorption or addition of the solution in the vessel 101. After the solution in the vessel 101 is cleaned, new solution needs to be added to the vessel 101 to flush the cells. Wash and cultivate, and then in the process of filling, maintain the tilt angle of the vessel 101 unchanged to complete the filling, and after the vessel 101 is filled, reduce the friction of the ball joint 203 by turning the knob, and then lightly touch the edge of the vessel 101 to make the vessel 101 shake, thereby driving the vibration spring 4 to shake, and use the reciprocating elastic change of the vibration spring 4 to cause the vessel 101 to shake, so as to make the solution in the vessel 101 mix evenly, reduce the time consumed by manually shaking the vessel 101, and improve the use efficiency of the vessel 101.

[0049] After the operation on the cells in the vessel 101 is completed, the friction force of the ball joint 203 is increased to ensure that the vessel 101 can maintain a relatively static state with the suction cup 301. At the same time, the user pulls the vessel 101 to the end away from the plane, thereby driving the vessel 101 and the connecting component 2 to move to the end away from the protection component 3, thereby driving the movable plate 204 to move within the inner ring of the fixed portion 304, releasing the blockage of the movable plate 204 on the protection component 3, allowing gas to enter the protection component 3, thereby prompting the protection component 3 to release the connection with the plane, making it easier for the user to move the culture dish equipment.

[0050] It should be noted that when the ball joint 203 rotates, the ball joint 203 only rotates and does not cause the movable plate 204 and the fixed portion 304 to move relative to each other.

[0051] At the same time, when the culture dish device enters the culture stage, the user folds the protection component 3, and at the same time, the vibration spring 4 and the movable rod 202 are compressed and limited, so that the connecting sleeve 302 covers the bottom of the vessel 101, and then the suction cup 301 covers the edge of the vessel 101, thereby achieving full coverage of the vessel 101, protecting the vessel 101 from the risk of collision, and ensuring that the vessel 101 will not be damaged during the cultivation process.

[0052] After the protective component 3 covers the edge of the container 101, the docking groove 3021 is located at the bottom of the container 101, and the docking sleeve 303 is located at the mouth of the container 101. Therefore, when multiple containers 101 are stacked, the docking sleeve 303 and the docking groove 3021 can correspond to each other, ensuring the stable position of the stacked containers 101.

[0053] By cooperating with the protective component 3 and the connecting component 2, the position of the vessel 101 can be fixed, thereby facilitating the use of the vessel 101 by the user and reducing the discomfort caused by operating the vessel 101. The rotation angle of the vessel 101 can be increased, so that the performance of the vessel 101 can be enhanced for the user. At the same time, the protective component 3 and the connecting component 2 cooperate with each other to assist the user to evenly mix the solution in the vessel 101 and fully contact the cells inside the vessel 101, thereby improving the culture efficiency of the culture component 1.

[0054] It should be noted that the terms "comprises," "includes," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements that are inherent to such process, method, article, or apparatus.

[0055] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A culture dish device for collecting mesenchymal stem cell factors, comprising a culture component (1), characterized in that: A connecting cavity (103) is provided at one end of the culture component (1), a connecting component (2) is provided in the connecting cavity (103), a protective component (3) is connected to the end of the connecting component (2) away from the culture component (1), and at least one vibration spring (4) is connected between the connecting cavity (103) and the protective component (3); The connection component (2) comprises a fixing sleeve (201), one end of the fixing sleeve (201) is fixedly connected in the connection cavity (103), and an end of the fixing sleeve (201) away from the culture component (1) is slidably provided with a movable rod (202); The protection component (3) comprises a suction cup (301), one side of the suction cup (301) is connected to a connecting sleeve (302), one end of the connecting sleeve (302) away from the suction cup (301) is connected to a fixing portion (304), and the fixing portion (304) is movably connected to the connecting component (2).

2. The culture dish device for collecting mesenchymal stem cell factors according to claim 1, characterized in that: The culture assembly (1) comprises a vessel (101), the connecting cavity (103) is located at one end of the vessel (101) away from the opening, and a reinforcing ring (102) is connected to one end of the vessel (101) away from the connecting cavity (103).

3. The culture dish device for collecting mesenchymal stem cell factors according to claim 1, characterized in that: The suction cup (301), the connecting sleeve (302) and the fixing portion (304) are all made of silicone material, and the fixing portion (304) is an annular body, wherein the diameter of the inner ring of the fixing portion (304) close to the culture component (1) is larger than the diameter of the fixing portion (304) away from the culture component (1).

4. The culture dish device for collecting mesenchymal stem cell factors according to claim 3, characterized in that: The connecting assembly (2) further comprises a movable plate (204), wherein the movable plate (204) is movably connected to the inner ring of the fixing portion (304), the movable plate (204) abuts against the inner ring of the fixing portion (304), and the movable plate (204) will not detach from the fixing portion (304).

5. The culture dish device for collecting mesenchymal stem cell factors according to claim 4, characterized in that: The connection assembly (2) further comprises a ball joint (203), the fixed end of the ball joint (203) being connected to one end of the movable rod (202), and the movable end of the ball joint (203) being connected to the movable plate (204).

6. The culture dish device for collecting mesenchymal stem cell factors according to claim 1, characterized in that: A docking sleeve (303) is connected to the end of the suction cup (301) away from the connecting sleeve (302), and a docking groove (3021) is provided on the inner side of the connecting sleeve (302), wherein the docking groove (3021) is close to the connection position between the connecting sleeve (302) and the suction cup (301), wherein the docking sleeve (303) is adapted to the docking groove (3021).

7. The culture dish device for collecting mesenchymal stem cell factors according to claim 6, characterized in that: A fold is provided at the connection position between the connecting sleeve (302) and the suction cup (301).

8. The culture dish device for collecting mesenchymal stem cell factors according to claim 1, characterized in that: An active cavity is provided in the fixed sleeve (201), an elastic reset device is connected to the active cavity, and the elastic reset device is in contact with the active rod (202).

9. The culture dish device for collecting mesenchymal stem cell factors according to claim 2, characterized in that: One end of the vibration spring (4) is connected to the cavity wall of the connection cavity (103), and the other end of the vibration spring (4) is connected to the connection sleeve (302).

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