Functional food for improving four highs based on combination of homology of medicine and food and probiotics

Abstract

The invention relates to the field of functional food, in particular to functional food for improving four highs based on combination of homology of medicine and food and probiotics. The functional food comprises the following components in parts by weight: 20 parts of white hyacinth beans, 35 parts of cortex lycii radicis, 25 parts of peanut coats, 30 parts of rhizoma smilacis glabrae, 25 parts of mulberry leaves, 10 parts of agilawood, 20 parts of Chinese prickly ash, 60 parts of smoked plums, 50 parts of rhizoma polygonati, 15 parts of fennel and bifidobacterium longum CCFM752 with the viable count of 1 * 10 < 8 > CFU / g. The viable count of the lactobacillus plantarum CW006 is 8 * 10 < 8 > CFU / g; and the viable count of the lactobacillus plantarum N13 is 15 * 10 < 8 > CFU / g. On the basis of network pharmacology and animal experiments, the composition can effectively act on metabolism signal channels related to four highs, and can regulate blood pressure, blood sugar, blood fat and uric acid levels so as to improve human health. In addition, the raw material components of the formula also have the characteristics of easiness in obtaining, low cost, easiness in production and convenience in taking, so that the formula has popularization value.

Description

Technical Field

[0001] The present invention relates to the field of functional foods, and in particular to functional foods based on the combination of medicine and food and probiotics for improving the levels of four highs. Background Art

[0002] With the changes in people's diet and living habits, the prevalence of hypertension, hyperlipidemia, hyperglycemia and hyperuric acid (the four highs) in my country's common metabolic syndrome has attracted much attention. Now the medical field uses lifestyle changes and drug treatment to improve or cure patients' diseases. In addition to exercise, one aspect of improving lifestyle that needs to be emphasized is the diet structure. Therefore, choosing natural foods suitable for high-risk patients and people with the four highs will bring more welfare to our country.

[0003] Although there are many drugs for the treatment of the four highs and related diseases, these drugs can only be used to stabilize the condition or relieve symptoms, and in most cases, patients need to take medication for a long time, which may cause damage to the patient's metabolism and other aspects and increase the economic burden. Now, the principle of health is prevention-oriented, combining prevention and treatment, and giving equal importance to Chinese and Western medicine, so that medicinal and edible foods, health foods and probiotic foods have become more and more popular among the people. So far, a large number of studies have confirmed that the active ingredients of single or compound Chinese herbal medicines have good regulatory ability on the four highs, and some regulatory mechanisms have also been elucidated. In addition, the human microecological community is also an important factor in the occurrence and development of diseases.

[0004] A Chinese patent document with authorization announcement number CN109805235B discloses that wet-granulating soybean peptide powder, soybean protein isolate powder, inulin, celery seed extract, barley powder, yeast powder, bonito extract, bitter melon powder, and olive powder and then using them for drinking can improve the four high levels of SD rats and some patients.

[0005] The main problems with foods for people with high cholesterol, high blood sugar and high blood pressure are low active ingredient content, poor bioavailability and high cost. In addition, there are currently no foods that are food-based and use probiotics to regulate the high cholesterol, high blood sugar and high blood pressure levels based on cost-effectiveness and network pharmacology screening. Therefore, developing a food combination that combines food-based and medicine-based herbs and probiotics to prevent and improve the high cholesterol, high blood sugar and high blood pressure levels has great economic and social benefits. Summary of the invention

[0006] In response to the problems existing in the background technology, a functional food based on the combination of medicine and food and probiotics to improve the levels of four highs is proposed. The raw material composition can effectively act on the metabolic signal pathways related to the four highs, and can regulate blood pressure, blood sugar, blood lipids and uric acid levels, thereby improving human health.

[0007] The present invention proposes a functional food based on the combination of medicine and food and probiotics to improve the four high levels. The weight proportions of the components include 20-40 parts of white hyacinth bean, 20-40 parts of Chinese wolfberry bark, 20-40 parts of peanut skin, 20-40 parts of smilax glabra, 20-30 parts of mulberry leaves, 10-30 parts of agarwood, 10-20 parts of pepper, 30-60 parts of black plum, 30-50 parts of polygonatum, 15-25 parts of fennel, and the number of viable bacteria is 1-15×10 8 CFU / g of Bifidobacterium longum CCFM752, and the number of viable bacteria is 1-15×10 8 CFU / g of Lactobacillus plantarum CW006 and the number of viable bacteria is 1-15×10 8 CFU / g of Lactobacillus plantarum N13.

[0008] Preferably, the weight proportions of the components include 20 parts of white hyacinth bean, 35 parts of Lycium bark, 25 parts of peanut skin, 30 parts of Smilax glabra, 25 parts of mulberry leaf, 10 parts of agarwood, 20 parts of pepper, 60 parts of black plum, 50 parts of polygonatum, 15 parts of fennel, and the number of viable bacteria is 1×10 8 CFU / g of Bifidobacterium longum CCFM752, and the number of viable bacteria was 8×10 8 CFU / g of Lactobacillus plantarum CW006 and the number of viable bacteria was 15×10 8 CFU / g of Lactobacillus plantarum N13.

[0009] Preferably, the weight proportions of the components include 30 parts of white hyacinth bean, 35 parts of Lycium bark, 20 parts of peanut skin, 40 parts of Smilax glabra, 20 parts of mulberry leaves, 20 parts of agarwood, 10 parts of pepper, 30 parts of black plum, 35 parts of Polygonatum sibiricum, 18 parts of fennel, and the number of viable bacteria is 8×10 8 CFU / g of Bifidobacterium longum CCFM752, and the number of viable bacteria was 1×10 8 CFU / g of Lactobacillus plantarum CW006 and the number of viable bacteria was 10×10 8 CFU / g of Lactobacillus plantarum N13.

[0010] Preferably, the weight proportions of the components include 25 parts of white hyacinth bean, 40 parts of Lycium bark, 40 parts of peanut skin, 25 parts of Smilax glabra, 28 parts of mulberry leaves, 25 parts of agarwood, 18 parts of pepper, 50 parts of black plum, 40 parts of polygonatum, 20 parts of fennel, and the number of viable bacteria is 10×10 8 CFU / g of Bifidobacterium longum CCFM752, and the number of viable bacteria was 15×10 8 CFU / g of Lactobacillus plantarum CW006 and the number of viable bacteria was 1×10 8 CFU / g of Lactobacillus plantarum N13.

[0011] Preferably, the weight proportions of the components include 40 parts of white hyacinth bean, 20 parts of Lycium bark, 30 parts of peanut skin, 20 parts of Smilax glabra, 30 parts of mulberry leaves, 30 parts of agarwood, 15 parts of Zanthoxylum bungeanum, 40 parts of black plum, 40 parts of Polygonatum sibiricum, 25 parts of fennel, and Bifidobacterium longum CCFM752 (with a viable count of 15×10 8 CFU / g), Lactobacillus plantarum CW006 (viable count was 10×10 8 CFU / g) and Lactobacillus plantarum N13 (viable count was 8×10 8 CFU / g).

[0012] Preferably, it also includes 5-20 parts of filler and 0.1-2 parts of lubricant.

[0013] Preferably, the filler is starch, mannitol, 13% stevia or erythrose and 26% microcrystalline cellulose.

[0014] Preferably, the lubricant is talc or magnesium stearate.

[0015] Preferably, the preparation method is as follows: remove impurities from the raw materials of each component, purify, dry, crush, and pass through a 120-mesh sieve, mix the powders according to weight proportion, and then add a filler and mix evenly; then granulate, dry, and granulate to obtain mixed granules; add a lubricant to the mixed granules, mix evenly, and prepare into corresponding dosage forms as needed.

[0016] Preferably, any suitable dosage form of oral preparation can be prepared according to needs.

[0017] Compared with the prior art, the present invention has the following beneficial technical effects:

[0018] The weight proportions of the components of the functional food for improving the four high levels based on the combination of medicine and food and probiotics proposed by the present invention include 20 parts of white hyacinth bean, 35 parts of Lycium bark, 25 parts of peanut skin, 30 parts of Smilax glabra, 25 parts of mulberry leaf, 10 parts of agarwood, 20 parts of pepper, 60 parts of black plum, 50 parts of polygonatum, 15 parts of fennel, and the number of viable bacteria is 1×10 8 CFU / g of Bifidobacterium longum CCFM752, and the number of viable bacteria was 8×10 8 CFU / g of Lactobacillus plantarum CW006 and the number of viable bacteria was 15×10 8 CFU / g of Lactobacillus plantarum N13. Based on network pharmacology and animal experiments, it is shown that the above composition can effectively act on the metabolic signal pathways related to the four highs, and can regulate blood pressure, blood sugar, blood lipids and uric acid levels, thereby improving human health. In addition, the raw material components of this prescription are also easy to obtain, low in cost, easy to produce, and convenient to take, so it has promotion value. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1This is a Venn diagram of the four high-grade medicinal materials screened based on the Symap database. There are 15 drugs that relieve the four highs;

[0020] Figure 2 The target network diagram of the Chinese medicine and food homologous components of the present invention, the selected targets are FDR (BH) < 0.05;

[0021] Figure 3 The KEGG pathways related to the four high diseases enriched according to the targets involved in the prescription of the present invention and FDR (BH) < 0.05;

[0022] Figure 4 The serum uric acid level of each group in the animal experiment of the present invention is shown in Figure 2. The variance analysis shows that the difference between the groups is p=3.9e -71 ;

[0023] Figure 5 This is the heart rate level diagram of each group in the animal experiment of the present invention. The variance analysis shows that the difference between the groups is p=2.0e -7 ;

[0024] Figure 6 The systolic blood pressure level of each group in the animal experiment of the present invention is shown in Figure 2. The variance analysis shows that the difference between the groups is p=2.5e -32 ;

[0025] Figure 7 The diastolic blood pressure level diagram of each group in the animal experiment of the present invention, the variance analysis shows that the difference between the groups is p = 1.4e -29 ;

[0026] Figure 8 This is a graph of blood glucose levels in each group of the animal experiment of the present invention. The variance analysis shows that the difference between the groups is p=2.5e -59 ;

[0027] Fig. 9 This is a graph of HDL-C levels in each group of the animal experiment of the present invention. Variance analysis shows that the difference between the groups is p=5.4e -31 ;

[0028] Fig.10 The LDL-C level diagram of each group in the animal experiment of the present invention, the variance analysis shows that the difference between the groups is p = 1.2e -20 ;

[0029] Fig.11 The TG level diagram of each group in the animal experiment of the present invention, the variance analysis shows that the difference between the groups is p = 4.9e -26 ;

[0030] Fig.12 The TC level diagram of each group in the animal experiment of the present invention, the variance analysis shows that the difference between the groups is p = 1.3e -44 . DETAILED DESCRIPTION

[0031] The raw material components involved in the present invention are as follows:

[0032] White lentil is the dried mature seed of Dolichos lablab L., a plant of the leguminous family. Nature and flavor and meridians: sweet, slightly warm. It enters the spleen and stomach meridians. Functions and indications: Strengthen the spleen and eliminate dampness, harmonize the middle and relieve heat. It is used for spleen and stomach weakness, loss of appetite, loose stools, excessive leucorrhea, vomiting and diarrhea due to summer heat and dampness, chest tightness and abdominal distension.

[0033] Rehmannia root bark is the root bark of Lycium chinense Mill. Nature and flavor and meridians: sweet; cold. Enters the lung and kidney meridians. Functions and indications: clears away heat; drains lung fire; cools blood. Treats yin deficiency and overwork fever; bone steaming and night sweats; fever due to malnutrition in children; cough and wheezing due to lung heat; vomiting blood; epistaxis; hematuria; thirst.

[0034] Peanut skin is the dried seed coat of the leguminous plant Arachis hypogaea L. Nature and flavor and meridians: sweet, astringent, flat. It enters the spleen and lung meridians. Functions and indications: stop bleeding, disperse blood stasis, and reduce swelling. It is used for hemophilia, hemophilia-like disease, primary and secondary thrombocytopenic purpura, hemorrhagic disease of liver disease, postoperative bleeding, cancer bleeding, bleeding in the stomach, intestines, lungs, uterus, etc.

[0035] Smilax glabra Roxb. is the dried rhizome of the plant Smilax glabra Roxb. of the Liliaceae family. Nature and flavor and meridians: sweet, light, flat. It enters the liver and stomach meridians. Functions and indications: remove dampness, detoxify, and open up the joints. It is used for damp-heat stranguria, leucorrhea, carbuncle, scrofula, scabies, syphilis, and limb cramps and muscle and bone pain caused by mercury poisoning.

[0036] Mulberry leaf is the dried leaf of Morus alba L., a plant of the Moraceae family. Nature and flavor and meridians: sweet, bitter, cold. It enters the lung and liver meridians. Functions and indications: dissipate wind-heat, clear the lungs and moisten dryness, clear the liver and improve eyesight. It is used for wind-heat cold, lung heat and dry cough, dizziness and headache, red and blurred eyes.

[0037] Aquilaria sinensis (Lour.) Gilg, a plant of the Thymelaeaceae family, contains resinous wood. Nature and flavor and meridians: pungent, bitter, slightly warm. It enters the spleen, stomach, and kidney meridians. Functions and indications: promotes qi and relieves pain, warms the middle and stops vomiting, and relieves asthma. It is used for chest and abdominal distension and pain, vomiting and hiccups due to stomach cold, and asthma due to kidney deficiency and qi reversal.

[0038] Zanthoxylum schinifoliumSieb.et Zucc. or Zanthoxylum bungeanumMaxim., a plant of Rutaceae, is a medicine made from the dried and mature peel (Zanthoxylum) and seeds (Zanthoxylum bungeanumMaxim. Nature and flavor and meridians: pungent, warm. It enters the spleen, stomach, and kidney meridians. Functions and indications: warming the middle and relieving pain, killing insects and relieving itching. It is used for cold pain in the abdomen, vomiting and diarrhea, abdominal pain caused by worms, and ascariasis; external treatment of eczema and itching.

[0039] Wumei is the dried nearly mature fruit of Prunus mume (Sieh.) Sieb.et Zucc. of the Rosaceae family. Nature and flavor and meridians: sour, astringent, neutral. It enters the liver, spleen, lung, and large intestine meridians. Functions and indications: astringent to the lungs, astringent to the intestines, promoting the production of body fluids, and calming ascariasis. It is used for chronic cough due to lung deficiency, chronic diarrhea and loose bowels, deficiency-heat and thirst, ascariasis due to vomiting and abdominal pain, and biliary ascariasis.

[0040] Polygonatum sibiricum is the dried rhizome of Polygonatum kingianum Coll.et Hemsl., Polygonatum sibiricum Red. or Polygonatum cyrtonema Hua of the Liliaceae family. Nature and flavor and meridians: sweet, neutral. It enters the spleen, lung and kidney meridians. Functions and indications: replenishing qi and nourishing yin, strengthening the spleen, moistening the lungs and benefiting the kidneys. It is used for spleen and stomach weakness, fatigue, dry mouth and poor appetite, dry cough due to lung deficiency, insufficient essence and blood, and internal heat and thirst.

[0041] Fennel is the dried mature fruit of Foeniculum vulgare Mill., a plant of the Umbelliferae family. Nature and flavor and meridians: pungent, warm. It enters the liver, kidney, spleen, and stomach meridians. Functions and indications: dispelling cold and relieving pain, regulating qi and harmonizing the stomach. It is used for abdominal pain caused by cold hernia, testicular prolapse, dysmenorrhea, cold pain in the lower abdomen, abdominal distension and pain, loss of appetite, vomiting and diarrhea, and testicular hydrocele. Salted fennel warms the kidneys, dispels cold and relieves pain. It is used for abdominal pain caused by cold hernia, testicular prolapse, and abdominal pain caused by cold menstruation.

[0042] Bifidobacterium longum is a strictly anaerobic Gram-positive bacterium of the genus Bifidobacterium, which is widely present in the digestive tract, vagina and oral cavity of humans and animals. It has multiple health functions, which can regulate intestinal flora, enhance immune function and relieve allergic reactions. Without changing cell viability, the supernatant of Bifidobacterium longum CCFM752 increased intracellular antioxidant capacity, enhanced intracellular catalase activity and reduced NADPH oxidase activation. As a colonizing bacterium in the intestine, Bifidobacterium longum has been shown to relieve colitis in mice and can be used as an alternative or auxiliary method for treating IBD.

[0043] Lactobacillus plantarum belongs to the genus Lactobacillus in the family Lactobacillaceae and is Gram-positive. Lactobacillus plantarum is a probiotic bacteria group in the human gastrointestinal tract. Its metabolism can produce a variety of natural antibacterial substances such as organic acids, bacteriocins, hydrogen peroxide, and diacetylphthalide. It has multiple functions such as maintaining the balance of intestinal flora, lowering cholesterol levels, improving the body's immunity, and promoting the absorption of nutrients. Lactobacillus plantarum CW006 has proteolytic activity and produces angiotensin-converting enzyme inhibitory peptides by fermenting raw milk to achieve anti-hypertensive effects. Therefore, it can be used as a starting bacteria in the dairy industry and applied to various health products and added to pharmaceutical products as a therapeutic substance. Lactobacillus plantarum N13 has acid resistance and bile salt resistance, has high adhesion to intestinal epithelial cell line HT-29 cells, and has the ability to inhibit EIEC growth and inhibit EIEC adhesion and invasion of intestinal epithelial cell line HT-29 cells. Lactobacillus plantarum N13 may be used to prepare sour soybean milk, fermented oatmeal beverages, fermented vegetables, fermented silage, lactic acid bacteria milk beverages and fermented milk.

[0044] Embodiment 1

[0045] The functional food proposed by the present invention improves the four high levels based on the combination of medicine and food and probiotics, and the weight proportions of its components include 20 parts of white hyacinth bean, 35 parts of Lycium bark, 25 parts of peanut skin, 30 parts of Smilax glabra, 25 parts of mulberry leaf, 10 parts of agarwood, 20 parts of pepper, 60 parts of black plum, 50 parts of polygonatum, 15 parts of fennel, and the number of viable bacteria is 1×10 8 CFU / g of Bifidobacterium longum CCFM752, and the number of viable bacteria was 8×10 8 CFU / g of Lactobacillus plantarum CW006 and the number of viable bacteria was 15×10 8 CFU / g of Lactobacillus plantarum N13.

[0046] Embodiment 2

[0047] The functional food proposed by the present invention improves the four high levels based on the combination of medicine and food and probiotics, and the weight proportions of its components include 30 parts of white hyacinth bean, 35 parts of Lycium bark, 20 parts of peanut skin, 40 parts of Smilax glabra, 20 parts of mulberry leaves, 20 parts of agarwood, 10 parts of Zanthoxylum bungeanum, 30 parts of black plum, 35 parts of Polygonatum sibiricum, 18 parts of fennel, and the number of viable bacteria is 8×10 8 CFU / g of Bifidobacterium longum CCFM752, and the number of viable bacteria was 1×10 8 CFU / g of Lactobacillus plantarum CW006 and the number of viable bacteria was 10×10 8 CFU / g of Lactobacillus plantarum N13.

[0048] Embodiment 3

[0049] The functional food proposed by the present invention is based on the combination of medicine and food with probiotics to improve the four high levels. The weight proportions of its components include 25 parts of white hyacinth bean, 40 parts of Lycium bark, 40 parts of peanut skin, 25 parts of Smilax glabra, 28 parts of mulberry leaves, 25 parts of agarwood, 18 parts of pepper, 50 parts of black plum, 40 parts of polygonatum, 20 parts of fennel, and the number of viable bacteria is 10×10 8 CFU / g of Bifidobacterium longum CCFM752, and the number of viable bacteria was 15×10 8 CFU / g of Lactobacillus plantarum CW006 and the number of viable bacteria was 1×10 8 CFU / g of Lactobacillus plantarum N13.

[0050] Embodiment 4

[0051] The functional food proposed by the present invention improves the four high levels based on the combination of medicine and food and probiotics, and its components include 40 parts of white hyacinth bean, 20 parts of Lycium bark, 30 parts of peanut skin, 20 parts of Smilax glabra, 30 parts of mulberry leaves, 30 parts of agarwood, 15 parts of Zanthoxylum bungeanum, 40 parts of black plum, 40 parts of polygonatum, 25 parts of fennel, Bifidobacterium longum CCFM752 (the number of viable bacteria is 15×10 8 CFU / g), Lactobacillus plantarum CW006 (viable count was 10×10 8 CFU / g) and Lactobacillus plantarum N13 (viable count was 8×10 8 CFU / g).

[0052] Embodiment 5

[0053] Based on Examples 1, 2, 3 and 4, this example proposes a method for preparing a functional food based on the combination of medicine and food and probiotics to improve the four high levels, and the steps are as follows: remove impurities from the raw materials of each component, purify, dry, crush, and pass through a 120-mesh sieve, mix the powders according to weight proportion, and then add 5-20 parts of filler (starch, mannitol, 13% stevioside or erythrose and 26% microcrystalline cellulose) and mix evenly; then granulate, dry, and granulate to obtain mixed granules; add 0.1-2 parts of lubricant (talc, magnesium stearate) to the mixed granules, mix evenly, and prepare into corresponding dosage forms as needed.

[0054] It should be further noted that any suitable dosage form of oral preparations can be prepared as required, including tablets, capsules, powders, granules, granules, effervescent tablets or sachets, etc.

[0055] Based on Examples 1, 2, 3 and 4, Comparative Examples 1-2 are proposed as follows:

[0056] (1) Comparative Example 1: 20 parts of white hyacinth bean, 35 parts of Chinese wolfberry bark, 25 parts of peanut skin, 30 parts of smilax glabra, 25 parts of mulberry leaves, 10 parts of agarwood, 20 parts of Zanthoxylum bungeanum, 60 parts of black plum, 50 parts of polygonatum and 15 parts of fennel, wherein the difference between Comparative Example 1 and Example 1 is the different ingredients, that is, Comparative Example 1 does not contain probiotics, and the preparation method is the same as above.

[0057] (2) Comparative Example 2: 35 parts of Lycium bark, 30 parts of Rhizoma Smilacis Glabrae, 10 parts of Aquilaria sinensis, 50 parts of Polygonatum sibiricum, and Bifidobacterium longum CCFM752 (viable count of 10 8 CFU / g), Lactobacillus plantarum CW006 (viable count of 10 8 CFU / g) and Lactobacillus plantarum N13 (viable count was 10 8 CFU / g). The difference between Comparative Example 2 and Example 1 is that drugs with less effect on target genes related to metabolic pathways are removed, including white lentil, peanut skin, mulberry leaf, prickly ash, black plum and cumin.

[0058] In this experiment, 6-week-old KM mice were selected, both male and female, SPF grade, and weighing (28±2) g. The male and female mice were randomly divided into hyperuricemia animal model group, hypertension animal model group, hyperlipidemia animal model group, hyperglycemia animal model group and blank control group.

[0059] In the blank control group, 10 mice were selected and fed with normal feed after 3 days of adaptive feeding.

[0060] The model group was modeled as follows:

[0061] 1. Animal model of hyperuricemia. Several mice were intraperitoneally injected with hypoxanthine (0.3 g kg -1 ) + subcutaneous injection of potassium oxonate (0.05 g kg -1 ), the model was replicated. On the 7th day of modeling, the model mice were tested to see whether the model was successful, and 10 mice in each group with successful modeling were taken for the experiment.

[0062] 2. Animal model of hypertension. Several mice were gavaged with L-NNA (700 mg kg -1 ) for 20 consecutive days to replicate the model. On the 7th day of modeling, the model mice were tested to see if the model was successful, and 10 mice in each group with successful modeling were tested.

[0063] 3. Animal model of hyperlipidemia. Several mice were fed a high-fat diet and gavaged with 1 million IU kg daily. -1 VitD3 (dissolved in rapeseed oil, gavage volume 20mL·kg -1 ), the model was replicated. On the 7th day of modeling, the model mice were tested to see whether the model was successful, and 10 mice in each group with successful modeling were taken for the experiment.

[0064] 4. Hyperglycemia animal model. Several mice were fed with a high-fat and high-sugar diet for 2 weeks, fasted for 7 hours, and intraperitoneally injected with 100 mg / kg of streptozotocin (STZ). -1 , model replication. On the 7th day of modeling, the model mice were tested to see whether the modeling was successful, and 10 mice in each group with successful modeling were selected for the experiment.

[0065] The mice in each group with successful modeling were selected and the functional food was mixed with boiling water according to the ratio of the embodiment and the comparative example and gavage was performed. The blank group was gavage with an equal amount of normal saline. The drug was administered once a day at a dose of 300 mg / kg. After continuous administration for 14 days, blood was collected from the eye sockets 1 hour after administration, and the blood was allowed to stand at room temperature for 30 minutes, centrifuged at 4°C, and the upper serum was taken.

[0066] The serum uric acid level of the hyperuricemia animal model group was determined using a fully automatic biochemical analyzer. The high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), and total cholesterol (TC) in the serum of the hyperlipidemia animal model group mice were determined using the Nanjing Jiancheng blood lipid determination kit. The mice were placed in a sphygmomanometer stand, and the heart rate (HR), systolic blood pressure (SBP), and diastolic blood pressure (DBP) of the hypertensive animal model group mice were measured by tail pressure method. Each mouse was measured 5 times in a row and the average value was taken. The tail vein blood of the mice was collected, and the blood glucose level of the hyperglycemia animal model group was determined using a Roche blood glucose meter (MY-20ACCU-CHEK activity, Roche Diagnostic Products Co., Ltd., Shanghai, China).

[0067] All experimental data were expressed as mean ± standard deviation, and the data were statistically processed using SPSS26.0 statistical analysis software. One-way analysis of variance was used for comparison between groups, and Turkey HSD post hoc test was used for two comparisons. The test level was set at α = 0.05, P>0.05 was insignificant, and P<0.05 was significant.

[0068] Compared with the blank group, the uric acid / blood pressure / blood lipid / blood sugar levels in the model group were significantly increased (P<0.05), indicating that the model was successfully established, as shown in Table 1. Compared with the model group, the uric acid / blood pressure / blood lipid / blood sugar levels in the Example 1-4 groups were significantly decreased (P<0.01), indicating that the composition prepared in this application can effectively improve the four high levels, among which the effect of Example 4 is the best, as shown in Tables 1 and Figure 4-12 Among them, changing the components will affect the efficacy of the composition. For Comparative Example 1 and Comparative Example 2, the efficacy of the composition obtained is significantly weakened, as shown in Tables 1 and Figure 4-12.

[0069] Table 1 Effects of different prescriptions on uric acid / blood pressure / blood lipid / blood sugar levels in mice (X±s, n=10)

[0070]

[0071]

[0072] #Compared with the blank control group: P < 0.05; *Compared with the model control group: P < 0.05; △Compared with the Example 1 group: P < 0.05, ※Compared with the Example 4 group: P < 0.05.

[0073] Traditional Chinese medicine has the characteristics of multiple components, multiple targets, and holistic effects. Network pharmacology is similar to the efficacy of traditional Chinese medicine. It has become a trend to use network pharmacology technology to study the pharmacodynamic basis and molecular mechanism of traditional Chinese medicine. Network pharmacology technology can help researchers focus on the proteins, genes and pathways that need to be studied, and has become a commonly used screening tool for researchers. This patent uses the Symap database (http: / / www.symmap.org) to retrieve traditional Chinese medicine related to hyperglycemia, hyperlipidemia, hypertension and hyperuricemia, and then takes the intersection of traditional Chinese medicine related to the four diseases, and selects the medicines recorded in the catalog of medicinal and edible materials for prescription and analysis. Prescriptions are formulated by retrieving probiotics related to hyperglycemia, hyperlipidemia, hypertension and hyperuricemia in the Pubmed database. KEGG pathway and protein interaction network analysis is performed on prescription-related targets based on big data.

[0074] The 10 medicinal materials involved in the present invention are from the Symap database and the catalog of medicinal and edible homology. Figure 1 The Venn diagram of the four high-dose medicinal materials is shown. The intersection of these 10 medicinal materials with the catalog of medicinal and edible homologous materials is used to obtain a food combination consisting of 10 foods, namely white hyacinth bean, lycium bark, peanut skin, smilax glabra, mulberry leaf, agarwood, prickly ash, black plum, polygonatum, and cumin. Then, based on the conditions of FDR (BH) < 0.05 and Symmap value > 1, the protein-protein interaction network of the targets of the 10 foods screened by the Symmap database that exert pharmacological activity is constructed. It can be seen that the action network is mainly enriched in the Ras (Rasassociated with Diabetes, RAD) and MutS homolog (MutS Homolog, MSH) family genes, see Figure 2The RAD family mainly regulates myocardium, skeletal muscle and diabetes, while the MSH family mainly participates in DNA base mismatch repair. In order to obtain the KEGG pathways involved in the targets of traditional Chinese medicine, the pathways with FDR (BH) < 0.05 were used as the screening criteria. The results showed that these targets were enriched in the diabetes pathway, hypertrophic cardiomyopathy, calcium signaling pathway, platelet activation and other KEGG metabolic pathways related to the four high diseases, see Figure 3 From the perspective of bioinformatics analysis, the rationality of the present invention's formula and its potential to assist in preventing or improving the four highs are fully demonstrated. Figure 3 .

[0075] Literature search shows that the probiotics Bifidobacterium longum CCFM752, Lactobacillus plantarum CW006 and Lactobacillus plantarum N13 not only have the functions of regulating intestinal flora, but also can increase the body's antioxidant capacity to further prevent various diseases, such as the four highs, aortic disease, cancer, etc.

[0076] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited thereto, and various changes can be made within the knowledge scope of technicians in the relevant technical field without departing from the purpose of the present invention.

Claims

1. A functional food based on the combination of medicine and food and probiotics to improve the four high levels, characterized by: The weight proportions of the components include 20-40 parts of white hyacinth bean, 20-40 parts of Chinese wolfberry bark, 20-40 parts of peanut skin, 20-40 parts of smilax glabra, 20-30 parts of mulberry leaf, 10-30 parts of agarwood, 10-20 parts of pepper, 30-60 parts of black plum, 30-50 parts of polygonatum, 15-25 parts of fennel, and the number of viable bacteria is 1-15×10 8 CFU / g of Bifidobacterium longum CCFM752, and the number of viable bacteria is 1-15×10 8 CFU / g of Lactobacillus plantarum CW006 and the number of viable bacteria is 1-15×10 8 CFU / g of Lactobacillus plantarum N13.

2. The functional food based on the combination of medicine and food and probiotics to improve the levels of four highs according to claim 1, characterized in that: The weight proportions of the components include 20 parts of white hyacinth bean, 35 parts of Lycium bark, 25 parts of peanut skin, 30 parts of Smilax glabra, 25 parts of mulberry leaves, 10 parts of agarwood, 20 parts of pepper, 60 parts of black plum, 50 parts of polygonatum, 15 parts of fennel, and the number of viable bacteria is 1×10 8 CFU / g of Bifidobacterium longum CCFM752, and the number of viable bacteria was 8×10 8 CFU / g of Lactobacillus plantarum CW006 and the number of viable bacteria was 15×10 8 CFU / g of Lactobacillus plantarum N13.

3. The functional food for improving the four high levels based on the combination of medicine and food and probiotics according to claim 1, characterized in that: The weight proportions of the components include 30 parts of white hyacinth bean, 35 parts of Lycium bark, 20 parts of peanut skin, 40 parts of Smilax glabra, 20 parts of mulberry leaves, 20 parts of agarwood, 10 parts of Zanthoxylum bungeanum, 30 parts of black plum, 35 parts of Polygonatum sibiricum, 18 parts of fennel, and the number of viable bacteria is 8×10 8 CFU / g of Bifidobacterium longum CCFM752, and the number of viable bacteria was 1×10 8 CFU / g of Lactobacillus plantarum CW006 and the number of viable bacteria was 10×10 8 CFU / g of Lactobacillus plantarum N13.

4. The functional food for improving the four high levels based on the combination of medicine and food and probiotics according to claim 1, characterized in that: The weight proportions of the components include 25 parts of white hyacinth bean, 40 parts of Lycium bark, 40 parts of peanut skin, 25 parts of Smilax glabra, 28 parts of mulberry leaves, 25 parts of agarwood, 18 parts of pepper, 50 parts of black plum, 40 parts of polygonatum, 20 parts of fennel, and the number of viable bacteria is 10×10 8 CFU / g of Bifidobacterium longum CCFM752, and the number of viable bacteria was 15×10 8 CFU / g of Lactobacillus plantarum CW006 and the number of viable bacteria was 1×10 8 CFU / g of Lactobacillus plantarum N13.

5. The functional food for improving the four high levels based on the combination of medicine and food and probiotics according to claim 1, characterized in that: The weight proportions of the components include 40 parts of white hyacinth bean, 20 parts of Lycium bark, 30 parts of peanut skin, 20 parts of Smilax glabra, 30 parts of mulberry leaves, 30 parts of agarwood, 15 parts of Zanthoxylum bungeanum, 40 parts of black plum, 40 parts of Polygonatum sibiricum, 25 parts of fennel, Bifidobacterium longum CCFM752 (with a viable count of 15×10 8 CFU / g), Lactobacillus plantarum CW006 (viable count was 10×10 8 CFU / g) and Lactobacillus plantarum N13 (viable count was 8×10 8 CFU / g).

6. The functional food based on the combination of medicine and food and probiotics to improve the levels of four highs according to any one of claims 1 to 5, characterized in that: It also includes 5-20 parts of filler and 0.1-2 parts of lubricant.

7. The functional food for improving the four high levels based on the combination of medicine and food and probiotics according to claim 6, characterized in that: The fillers are starch, mannitol, 13% stevia or erythrose and 26% microcrystalline cellulose.

8. The functional food for improving the four high levels based on the combination of medicine and food and probiotics according to claim 7, characterized in that: Lubricants are talc and magnesium stearate.

9. The functional food for improving the four high levels based on the combination of medicine and food and probiotics according to claim 6, characterized in that: The preparation method is as follows: The raw materials of each component are removed from impurities, purified, air-dried, crushed, and passed through a 120-mesh sieve. The powders are mixed evenly according to weight proportions, and then fillers are added and mixed evenly. Then, granulation, drying, and granulation are performed to obtain mixed granules. A lubricant is added to the mixed granules and mixed evenly.

10. The functional food for improving the four high levels based on the combination of medicine and food and probiotics according to claim 9, characterized in that: Oral preparations can be prepared in any suitable dosage form as desired.

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