Cleaning device and cleaning method

Abstract

The invention discloses a cleaning device and a cleaning method, and relates to the technical field of medical instruments, the cleaning device comprises a base, a cleaning tank is arranged on the top surface of the base, and an ultrasonic vibrator is arranged at the bottom of the cleaning tank; a cleaning head is arranged at the top of the cleaning tank, a plurality of liquid suction needles and liquid injection needles are arranged at the bottom of the cleaning head, the cleaning head is connected with a lifting guide rail through a hanging plate, the lifting guide rail is fixedly arranged on the plate face of a mounting plate, and the end, close to the lifting guide rail, of the hanging plate is in threaded connection to a lead screw. The lead screw is connected to an output shaft of a first motor, and the first motor is fixedly arranged at the bottom of the base. A microplate carrier is arranged on one side of the cleaning tank, a microplate is arranged on the microplate carrier, and the microplate carrier is connected to the transverse guide rail in a sliding mode; according to the ultrasonic cleaning device, impurities and crystals attached to the interior of the steel needle of the cleaning head are dissolved and broken through the cavitation effect of ultrasonic waves, and then blocked parts or residual parts are dredged and cleaned through normal liquid injection and liquid suction.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a cleaning device and a cleaning method. Background Art

[0002] The plate washer is mainly used in the ELISA experiment to wash away unbound antigens or antibodies and impurities so that only the antigen-antibody complex formed by specific binding remains on the solid phase carrier. The washing effect will affect the accuracy of subsequent test results.

[0003] The main component of washing is the cleaning head. Because the aperture of its injection needle and aspiration needle is small, it is easy to get clogged during use. The cleaning solution is generally a phosphate solution. After the filling is completed, the injection needle is in a full state. During the static process, the front end of the injection needle evaporates and crystallizes, resulting in narrow aperture or even blockage. The blockage position is generally within 2mm of the front end of the injection needle. During the aspiration process of the aspiration needle, the residual flocculent protein will agglomerate at the end of the needle. After the aspiration is completed, the inside of the aspiration needle is completely in contact with the air, and the agglutinated protein will dry up into blocks, resulting in narrow aperture or even blockage. The blockage position is generally located around the root of the end of the injection needle. When the injection needle and the aspiration needle are blocked, no injection or aspiration will occur, which will cause mutual contamination between the holes, making it impossible to obtain accurate results in the test.

[0004] When the cleaning head is clogged, the conventional solution is to remove the cleaning head and use a steel wire of corresponding diameter to mechanically clear it to make it smooth. However, the mechanical clearing process will cause scratches inside the needle, increasing the risk of subsequent impurity accumulation, resulting in repeated clearing and increasing the uncertainty of experimental results. Summary of the invention

[0005] In view of the above deficiencies in the prior art, the object of the present invention is to provide a cleaning device and a cleaning method, which can solve the problem that the mechanical dredging method used in the prior art may cause scratches inside the needle and increase the risk of subsequent impurity accumulation.

[0006] In order to achieve the above object, the present invention provides the following technical solutions: A cleaning device comprises a base, a cleaning tank is provided on the top surface of the base, an ultrasonic vibrator is provided at the bottom of the cleaning tank; a cleaning head is provided on the top of the cleaning tank, a plurality of aspiration needles and injection needles are provided at the bottom of the cleaning head, the cleaning head is connected to a lifting guide rail through a hanging plate, the lifting guide rail is fixed on the plate surface of a mounting plate, one end of the hanging plate close to the lifting guide rail is threadedly connected to a screw rod, the screw rod is connected to the output shaft of a motor, and the motor is fixed at the bottom of the base; a microplate carrier is provided on one side of the cleaning tank, a microplate is provided on the microplate carrier, and the microplate carrier is slidably connected to a transverse guide rail.

[0007] Preferably, a second motor is provided at the bottom of the base, a first pulley is connected to the output shaft of the second motor, the first pulley is connected to a second pulley via a belt, the second pulley is provided on the base, and the microplate carrier is fixedly connected to the belt.

[0008] Preferably, a spring block is provided on the corner of one end of the microplate carrier away from the cleaning tank, and the spring block is connected to the microplate carrier through a driving screw. A torsion spring is sleeved on the driving screw, and the torsion spring is located between the microplate carrier and the spring block. A limit toggle screw is provided at the bottom of the spring block, and the limit toggle screw cooperates with the limit column on the top surface of the base to realize the opening of the spring block.

[0009] Preferably, the screw rod and the suspension plate are connected via a screw rod nut, and the suspension plate and the cleaning head are connected via bolts.

[0010] Preferably, a plurality of channels are opened in the cleaning head, a plurality of aspiration needle holes and injection needle holes are arranged at the bottom of the channels, aspiration needles and injection needles are respectively installed in the aspiration needle holes and injection needle holes, and the aspiration needles and injection needles are connected to their corresponding channels.

[0011] Preferably, the ultrasonic vibrator adopts 40KHz, the liquid aspiration needle is 1.5mm-2mm away from the bottom of the cleaning tank, and the liquid injection needle is immersed in the cleaning liquid for 1.5mm-2mm.

[0012] A cleaning method using a cleaning device comprises the following steps: S1, the microplate carrier moves to the right under the drive of motor 2 until the limit toggle screw contacts the limit column, and the spring block opens under the action of the torsion spring, placing the microplate on the microplate carrier; S2, motor 2 is activated again, driving the microplate carrier to move to the top of the cleaning tank. At this time, motor 1 is activated to drive the screw to rotate, thereby driving the suspension plate to move downward until the aspiration needle and injection needle at the bottom of the cleaning head are inserted into the reaction cup on the microplate, and cleaning is performed through liquid circuit control; S3. After the reaction cup is cleaned, the cleaning head moves up, and the microplate carrier moves to the right along the transverse guide rail driven by the second motor until the limit toggle screw contacts the limit column again, and the spring block opens under the action of the torsion spring, and the microplate can be taken away; S4. When cleaning the aspiration needle and the injection needle, the cleaning head is lowered into the cleaning tank, the aspiration needle of the cleaning head is 1.5mm-2mm away from the bottom of the cleaning tank, and distilled water is injected into the cleaning tank so that the injection needle of the cleaning head is immersed in the distilled water for 1.5mm-2mm; S5, then the aspiration needle aspirates for 1 second, at which time the aspiration needle and the internal channel of the cleaning head are soaked with distilled water, and then the ultrasonic vibrator is turned on to vibrate and clean the aspiration needle and the injection needle; S6. After cleaning for a period of time, the blockages in the aspiration needle and the injection needle can be cleaned out. Finally, the injection needle injects liquid for 10 seconds, and the aspiration needle absorbs liquid at the same time until the liquid in the cleaning tank is sucked away.

[0013] Compared with the prior art, the present invention has the following beneficial effects: the present invention uses ultrasonic waves to clean the aspiration needle and the injection needle. In the case of phosphate crystallization in the injection needle, the front end is soaked in liquid with a diameter of 1.5mm-2mm, and the high-frequency vibration of ultrasonic waves is used to accelerate the dissolution, so that the injection needle can be quickly restored to a smooth state; in the case of protein agglutination in the aspiration needle, the aspiration needle is soaked in liquid, and the cavitation effect of ultrasonic waves is used to crush the aggregates, and the aspiration and discharge are performed, so that the aspiration needle remains smooth. Compared with the mechanical dredging in the prior art, internal scratches are avoided and the hidden danger of accumulation of impurities is reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0015] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 is a cross-sectional view of the present invention; Figure 3 for Figure 2 Enlarged view of point A in the middle; Figure 4 It is a schematic diagram of the structure of the microplate carrier of the present invention; Figure 5 It is a schematic diagram of the internal structure of the cleaning head of the present invention; Figure 6 It is a schematic diagram of the placement structure of the aspiration needle and the injection needle of the present invention during the frequency test; Figure 7 It is a schematic diagram of the placement structure of the liquid aspiration needle of the present invention during height testing.

[0016] Description of reference numerals: 1-base, 2-cleaning tank, 3-cleaning head, 4-pipetting needle, 5-injection needle, 6-hanging plate, 7-lifting guide rail, 8-mounting plate, 9-screw, 10-motor 1, 11-microplate carrier, 12-microplate, 13-lateral guide rail, 14-motor 2, 15-pulley 1, 16-belt, 17-pulley 2, 18-spring, 19-plug screw, 20-torsion spring, 21-limit toggle screw, 22-limit column, 23-screw nut, 24-bolt, 25-channel. DETAILED DESCRIPTION

[0017] The following is a detailed description of the invention by way of examples in conjunction with the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the invention, rather than all of the embodiments. Embodiment 1

[0018] like Figures 1 to 5 As shown, the present invention discloses a cleaning device, including a base 1, a cleaning tank 2 is provided on the top surface of the base 1, an ultrasonic vibrator is provided at the bottom of the cleaning tank 2, and the ultrasonic vibrator adopts 40KHz; a cleaning head 3 is provided on the top of the cleaning tank 2, a plurality of channels 25 are opened in the cleaning head 3, a plurality of liquid suction pin holes and liquid injection pin holes are provided at the bottom of the channel 25, a liquid suction pin hole and a liquid injection pin hole are respectively installed in the liquid suction pin hole and the liquid injection pin hole, and the liquid suction pin 4 and the liquid injection pin 5 are both connected to their corresponding channels 25. During cleaning, the liquid suction pin 4 is 1.5mm-2mm away from the bottom of the cleaning tank 2, and the liquid injection pin 5 is immersed in the cleaning liquid for 1.5mm-2mm.

[0019] Furthermore, in this embodiment, the cleaning head 3 is connected to the lifting rail 7 through the hanging plate 6, the hanging plate 6 and the cleaning head 3 are connected by bolts 24, the lifting rail 7 is fixed on the plate surface of the mounting plate 8, the end of the hanging plate 6 close to the lifting rail 7 is threadedly connected to the screw 9, the screw 9 and the hanging plate 6 are connected by a screw nut 23, the screw 9 is connected to the output shaft of the motor 10, and the motor 10 is fixed at the bottom of the base 1; by setting the motor 10 and the screw 9, it is convenient to drive the hanging plate 6 to move up and down, and setting the lifting rail 7 is convenient to guide the movement of the hanging plate 6. A microplate carrier 11 is provided on one side of the cleaning tank 2, and a microplate 12 is provided on the microplate carrier 11, and the microplate carrier 11 is slidably connected to the transverse guide rail 13.

[0020] Furthermore, in this embodiment, a second motor 14 is provided at the bottom of the base 1, and a pulley 15 (not shown in the figure) is connected to the output shaft of the second motor 14, and the pulley 15 is connected to the second pulley 17 through a belt 16, and the second pulley 17 is provided on the base 1, and the microplate carrier 11 is fixedly connected to the belt 16. By providing the second motor 14, the first pulley 15, the belt 16 and the second pulley 17, it is beneficial to drive the microplate carrier 11 to move and realize the plate washing operation.

[0021] Furthermore, in the present embodiment, a spring block 18 is provided on the corner of one end of the microplate carrier 11 away from the cleaning tank 2, and the spring block 18 is connected to the microplate carrier 11 through a plug screw 19, and a torsion spring 20 is sleeved on the plug screw 19. The torsion spring 20 is located between the microplate carrier 11 and the spring block 18, and a limit-shifting screw 21 is provided at the bottom of the spring block 18. The limit-shifting screw 21 cooperates with the limit column 22 on the top surface of the base 1 to realize automatic opening of the spring block 18, thereby facilitating the disassembly and assembly of the microplate 12.

[0022] The present invention uses ultrasonic cavitation to loosen, dissolve and break impurities, crystals and protein coagulation attached to the steel needles of the cleaning head 3 under the action of liquid compression, and then dredges and cleans the blocked or residual parts through normal liquid injection and liquid suction. During the working process, it is necessary to ensure that the cleaning head 3 is filled with distilled water so that the cavitation can reach the inside of the cleaning head 3 to achieve thorough cleaning.

[0023] 1. Frequency selection test Test sample: three aspiration needles and three injection needles for the cleaning head Test steps: Use a 100,000-point balance to weigh the initial weight of the aspiration needle and injection needle. After soaking in the washing solution for 30 minutes, put them into the drying box for drying, and then Figure 6 Place them separately as shown, adjust different frequencies for cleaning, the cleaning time is 3 minutes, and put them into the oven for drying again after cleaning, and weigh the data; determine the residual amount based on the data difference.

[0024]

[0025] 2. Height Test Test sample: Six cleaning head aspiration needles Test steps: Place the tip of the pipette needle Figure 7 As shown, they are fixed in sequence, and the tail end of the pipette needle is wound with absorbent paper. After ultrasonic cleaning (40KHz) for 3 minutes, the amount of liquid ejected by "cavitation" is weighed using a 100,000-micrometer balance.

[0026]

[0027] It can be seen that the cleaning effect is best when the ultrasonic vibrator is selected at 40KHz, the liquid aspiration needle is 1.5mm-2mm away from the bottom of the cleaning tank, and the injection needle is immersed in distilled water for 1.5mm-2mm.

[0028] Embodiment 2 The present invention also discloses a cleaning method using the cleaning device, comprising the following steps: S1, the microplate carrier 11 moves to the right under the drive of the motor 2 14 until the limit toggle screw 21 contacts the limit column 22, and the spring block 18 opens under the action of the torsion spring 20, placing the microplate 12 on the microplate carrier 11; S2, the motor 2 14 is activated again, driving the microplate carrier 11 to move above the cleaning tank 2, at which time the motor 10 is activated, driving the screw 9 to rotate, thereby driving the suspension plate 6 to move downward, until the aspiration needle 4 and the injection needle 5 at the bottom of the cleaning head 3 extend into the reaction cup on the microplate 12, and cleaning is performed through liquid circuit control; S3, after the reaction cup is cleaned, the cleaning head 3 moves upward, and the microplate carrier 11 moves to the right along the transverse guide rail 13 driven by the motor 2 14 until the limit toggle screw 21 contacts the limit column 22 again, and the spring block 18 opens under the action of the torsion spring 20, and the microplate 12 can be taken away; S4, when cleaning the liquid suction needle 4 and the liquid injection needle 5, the cleaning head 3 is lowered into the cleaning tank 2, the liquid suction needle 4 of the cleaning head 3 is 1.5mm-2mm away from the bottom of the cleaning tank 2, and distilled water is injected into the cleaning tank 2, so that the liquid injection needle 5 of the cleaning head 3 is immersed in the distilled water for 1.5mm-2mm; S5, then the aspiration needle 4 aspirates the liquid for 1 second, at which time the aspiration needle 4 and the internal channel 25 of the cleaning head 3 are soaked with distilled water, and then the ultrasonic vibrator is turned on to vibrate and clean the aspiration needle 4 and the injection needle 5; S6. After cleaning for a period of time, the blockages in the liquid aspiration needle 4 and the liquid injection needle 5 can be cleaned out. Finally, the liquid injection needle 5 injects liquid for 10 seconds, and the liquid aspiration needle 4 absorbs liquid at the same time until the liquid in the cleaning tank 2 is sucked away.

[0029] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solution and application concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A cleaning device, comprising a base (1), characterized in that: A cleaning tank (2) is provided on the top surface of the base (1), and an ultrasonic vibrator is provided at the bottom of the cleaning tank (2); a cleaning head (3) is provided on the top of the cleaning tank (2), and a plurality of liquid aspiration needles (4) and liquid injection needles (5) are provided at the bottom of the cleaning head (3); the cleaning head (3) is connected to a lifting guide rail (7) via a hanging plate (6); the lifting guide rail (7) is fixed on the plate surface of a mounting plate (8); one end of the hanging plate (6) close to the lifting guide rail (7) is threadedly connected to a screw rod (9); the screw rod (9) is connected to an output shaft of a motor (10); and the motor (10) is fixed at the bottom of the base (1); a microplate carrier (11) is provided on one side of the cleaning tank (2); a microplate (12) is provided on the microplate carrier (11), and the microplate carrier (11) is slidably connected to a transverse guide rail (13).

2. A cleaning device as claimed in claim 1, characterized in that: A second motor (14) is provided at the bottom of the base (1), a first pulley (15) is connected to the output shaft of the second motor (14), the first pulley (15) is connected to a second pulley (17) via a belt (16), the second pulley (17) is provided on the base (1), and the microplate carrier (11) is fixedly connected to the belt (16).

3. A cleaning device as claimed in claim 2, characterized in that: A spring block (18) is provided on the corner of one end of the microplate carrier (11) away from the cleaning tank (2). The spring block (18) is connected to the microplate carrier (11) via a plug screw (19). A torsion spring (20) is sleeved on the plug screw (19). The torsion spring (20) is located between the microplate carrier (11) and the spring block (18). A limit-shifting screw (21) is provided at the bottom of the spring block (18). The limit-shifting screw (21) cooperates with a limit column (22) on the top surface of the base (1) to realize the opening of the spring block (18).

4. A cleaning device as claimed in claim 1, characterized in that: The screw rod (9) and the suspension plate (6) are connected via a screw rod nut (23), and the suspension plate (6) and the cleaning head (3) are connected via a bolt (24).

5. A cleaning device as claimed in claim 3, characterized in that: A plurality of channels (25) are provided in the cleaning head (3), a plurality of liquid suction needle holes and liquid injection needle holes are provided at the bottom of the channels (25), a liquid suction needle (4) and a liquid injection needle (5) are installed in the liquid suction needle holes and the liquid injection needle holes, respectively, and the liquid suction needle (4) and the liquid injection needle (5) are both connected to their corresponding channels (25).

6. A cleaning device as claimed in claim 5, characterized in that: The ultrasonic vibrator uses 40KHz, the liquid aspiration needle (4) is 1.5mm-2mm away from the bottom of the cleaning tank (2), and the liquid injection needle (5) is immersed in the cleaning liquid for 1.5mm-2mm.

7. A cleaning method using the cleaning device as claimed in claim 6, characterized in that: The following steps are involved: S1, the microplate carrier (11) moves to the right under the drive of the second motor (14) until the limit toggle screw (21) contacts the limit column (22), and the spring block (18) opens under the action of the torsion spring (20), placing the microplate (12) on the microplate carrier (11); S2, the motor 2 (14) is activated again, driving the microplate carrier (11) to move to the top of the cleaning tank (2), at which time the motor 1 (10) is activated, driving the screw (9) to rotate, thereby driving the suspension plate (6) to move downward until the aspiration needle (4) and the injection needle (5) at the bottom of the cleaning head (3) extend into the reaction cup on the microplate (12), and cleaning is performed through liquid circuit control; S3. After the reaction cup is cleaned, the cleaning head (3) moves upward, and the microplate carrier (11) moves to the right along the transverse guide rail (13) driven by the second motor (14) until the limit toggle screw (21) contacts the limit column (22) again, and the spring block (18) opens under the action of the torsion spring (20), and the microplate (12) can be removed; S4, when cleaning the aspiration needle (4) and the injection needle (5), the cleaning head (3) is lowered into the cleaning tank (2), the aspiration needle (4) of the cleaning head (3) is 1.5 mm to 2 mm away from the bottom of the cleaning tank (2), and distilled water is injected into the cleaning tank (2) so that the injection needle (5) of the cleaning head (3) is immersed in the distilled water for 1.5 mm to 2 mm; S5, the aspiration needle (4) then aspirates the liquid for 1 second, at which time the aspiration needle (4) and the internal channel (25) of the cleaning head (3) are soaked in distilled water, and then the ultrasonic vibrator is turned on to vibrate and clean the aspiration needle (4) and the injection needle (5); S6. After cleaning for a period of time, the obstructions in the aspiration needle (4) and the injection needle (5) can be cleaned out. Finally, the injection needle (5) injects liquid for 10 seconds, while the aspiration needle (4) absorbs liquid until the liquid in the cleaning tank (2) is sucked away.

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