Burkholderia cepacia and its method for extracting rare earth elements from leaching waste

Abstract

This invention relates to the field of environmentally friendly bio-rare earth recycling technology, and particularly to Burkholderia cepacia and its method for leaching rare earth elements from waste. Specifically, this invention provides a method for leaching rare earth elements from waste using microorganisms and their metabolites, solving the problems of large amounts of chemical reagents, high production costs, and serious environmental pollution in the chemical leaching process of rare earth waste. The strain provided by this invention has low cultivation costs, fast growth rate, simple leaching conditions, and the ability to leach rare earth ions from various wastes, showing good application prospects in the microbial recycling and reuse of rare earth waste.

Description

Technical Field

[0001] This invention relates to the field of environmentally friendly biological rare earth recycling technology, and in particular to a method for extracting rare earth elements from Burkholderia cepacia and its leaching waste. Background Technology

[0002] Rare earth elements, including fifteen lanthanides and scandium and yttrium, are widely used as important strategic resources in cutting-edge research and high-tech industries, and are known as "industrial gold." However, due to their uneven distribution and limited reserves, with the widespread application of rare earth elements in permanent magnet materials, industrial catalysis, and other fields, it has become essential to extract rare earth elements from these secondary rare earth resources.

[0003] Traditional methods for recovering rare earth elements (REEs) from urban minerals primarily employ physicochemical leaching methods using strong acids and organic solvents. However, these methods are complex, energy-intensive, and cause significant environmental pollution. Therefore, there is an urgent need to develop a clean and efficient new leaching technology to ensure the leaching rate of rare earth elements while minimizing environmental pollution.

[0004] Bioleaching using microorganisms is an environmentally friendly alternative. This method primarily dissolves rare earth elements (REEs) from a solid matrix using organic acids (such as citric acid and lactic acid) produced by heterotrophic bacteria or fungi. However, commercially available microorganisms such as yeast and *Gluconobacterium oxysporum* are inefficient and time-consuming in recovering REEs from spent FCC catalysts and NdFeB magnets. This is mainly because these microorganisms have poor tolerance to high concentrations of REEs. Therefore, screening and culturing robust microorganisms from the microbial communities associated with rare earth mines and rare earth waste is crucial for improving the bioaccumulation efficiency of REEs. However, there are few reports on bioleaching based on such microorganisms. Therefore, providing a novel *Burkholderia cepacia* strain capable of efficiently leaching REEs from waste has significant practical implications, offering a new option and approach for biometallurgy. Summary of the Invention

[0005] In view of this, the present invention provides a novel Burkholderia cepacia strain capable of efficiently leaching rare earth ions from waste, offering a new option and approach for biometallurgy. The present invention also provides a method for leaching rare earth elements from waste using microorganisms and their metabolites, solving problems such as large quantities of chemical reagents, high production costs, and severe environmental pollution associated with the chemical leaching of rare earth waste.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0007] In a first aspect, the present invention provides Burkholderia contaminans AHA-5, which has the accession number CGMCC No. 30396.

[0008] Secondly, the present invention also provides a method for culturing Burkholderia contaminans AHA-5, wherein Burkholderia contaminans AHA-5 is picked and cultured in Luria-Bertani liquid medium supplemented with 60 g / L glucose or in nutrient-deficient Luria-Bertani liquid medium supplemented with 60 g / L glucose at 30°C and 200 rpm for 2 to 4 days.

[0009] In the nutrient-deficient Luria-Bertani liquid medium, the original components of the LB medium account for 0.05% to 50%.

[0010] Thirdly, the present invention also provides one or more of the inactivated bacterial cells, exosomes, or metabolites of Burkholderia contaminans AHA-5.

[0011] Fourthly, the present invention also provides a method for preparing the metabolites of Burkholderia contaminans AHA-5, wherein Burkholderia contaminans AHA-5 is picked and cultured in Luria-Bertani liquid medium supplemented with 60 g / L glucose or in nutrient-deficient Luria-Bertani liquid medium supplemented with 60 g / L glucose at 30°C and 200 rpm for 2-4 days, the culture medium is collected and centrifuged at 8000 rpm for 5 min, and the supernatant is collected;

[0012] In the nutrient-deficient Luria-Bertani liquid medium, the original components of the LB medium account for 0.05% to 50%.

[0013] Fifthly, the present invention also provides metabolites obtained by the preparation method described above.

[0014] In a sixth aspect, the present invention also provides the application of any of the following in rare earth elements in leaching waste;

[0015] (I) Burkholderia contaminans AHA-5;

[0016] (II) One or more of the inactivated bacterial cells, exosomes or metabolites mentioned above;

[0017] (III) The metabolites mentioned above.

[0018] In some specific embodiments of the present invention, the waste includes one or more of waste FCC catalyst, waste NdFeB sludge, or waste NdFeB powder.

[0019] In some specific embodiments of the present invention, the main framework of the spent FCC catalyst is aluminosilicate, containing rare earth elements and / or transition elements, wherein the content of the rare earth elements and / or transition elements is 0.01% to 10%; the rare earth elements include, but are not limited to, lanthanum and / or cerium; the transition elements include, but are not limited to, nickel and / or vanadium.

[0020] The main component of the waste NdFeB sludge is NdFeB (N2Fe). 14 B) Magnets containing 1% to 30% lubricating oil;

[0021] The main component of the waste NdFeB powder is NdFeB (Nd2Fe). 14 B) Originates from various stages of industrial production of NdFeB magnets, including cutting, molding, or sintering processes.

[0022] In a seventh aspect, the present invention also provides an article of manufacture comprising any one of the following:

[0023] (I) Burkholderia contaminans AHA-5;

[0024] (II) One or more of the inactivated bacterial cells, exosomes or metabolites mentioned above;

[0025] (III) The aforementioned metabolites;

[0026] The products include microecological products, probiotic products, synbiotic products and / or postbiotic products.

[0027] Eighthly, the present invention also provides a method for leaching rare earth elements from leaching waste, comprising mixing any one of the following with the waste and incubating it;

[0028] (I) Burkholderia contaminans AHA-5;

[0029] (II) One or more of the inactivated bacterial cells, exosomes or metabolites mentioned above;

[0030] (III) The aforementioned metabolites;

[0031] (IV) The aforementioned articles.

[0032] In some specific embodiments of the present invention, the waste includes one or more of waste FCC catalyst, waste NdFeB sludge, or waste NdFeB powder;

[0033] Preferably, the main framework of the spent FCC catalyst is aluminosilicate, containing rare earth elements and / or transition elements, wherein the content of the rare earth elements and / or transition elements is 0.01% to 10%; the rare earth elements include, but are not limited to, lanthanum and / or cerium; the transition elements include, but are not limited to, nickel and / or vanadium.

[0034] The main component of the waste NdFeB sludge is NdFeB (N2Fe). 14 B) Magnets containing 1% to 30% lubricating oil;

[0035] The main component of the waste NdFeB powder is NdFeB (Nd2Fe). 14 B) Originates from various stages of industrial production of NdFeB magnets, including cutting, molding, or sintering processes.

[0036] Preferably, the liquid-to-solid ratio of the Burkholderia contaminans AHA-5 to the spent FCC catalyst is 1:(2-500);

[0037] The liquid-to-solid ratio of the Burkholderia contaminans AHA-5 to the waste NdFeB sludge is 1:(4-500);

[0038] The liquid-to-solid ratio of the Burkholderia contaminans AHA-5 to the waste NdFeB powder is 1:(4-500);

[0039] Preferably, the liquid-to-solid ratio of the metabolite to the spent FCC catalyst is 1:(2-500);

[0040] The liquid-to-solid ratio of the metabolites to the waste NdFeB sludge is 1:50.

[0041] The liquid-to-solid ratio of the metabolite to the waste NdFeB powder is 1:50.

[0042] Preferably, the incubation conditions are 30°C and 200 rpm for 2 to 8 days.

[0043] This invention provides a method for leaching rare earth elements from waste using microorganisms and their metabolites, solving problems such as large amounts of chemical reagents, high production costs, and severe environmental pollution associated with the chemical leaching of rare earth waste. The strains provided in this invention have low cultivation costs, rapid growth rates, and simple leaching conditions, and are capable of leaching rare earth ions from various types of waste, showing promising application prospects in the microbial recycling and reuse of rare earth waste.

[0044] Biological Preservation Instructions

[0045] Strain: AHA-5; deposit date: April 22, 2024; deposit number: CGMCC No. 30396; classification name: Burkholderia contaminans; depositary institution: China General Microbiological Culture Collection Center; depositary address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing. Attached Figure Description

[0046] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below.

[0047] Figure 1 The leaching amount of rare earth elements in waste FCC catalyst by strain AHA-5 is shown.

[0048] Figure 2 A bar chart showing the leaching amount of rare earth elements from waste NdFeB sludge by strain AHA-5;

[0049] Figure 3 The bar chart shows the leaching amount of rare earth elements from waste NdFeB powder by strain AHA-5. Detailed Implementation

[0050] This invention discloses a method for extracting rare earth elements from Burkholderia cepacia and its leaching waste. Those skilled in the art can refer to this document and appropriately modify the process parameters to achieve the desired result. It is particularly important to note that all similar substitutions and modifications are obvious to those skilled in the art and are considered to be included in this invention. The methods and applications of this invention have been described through preferred embodiments. Those skilled in the art can clearly modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit, and scope of this invention to realize and apply the technology of this invention.

[0051] The present invention adopts the following technical solution:

[0052] (1) Screening of mineral-leaching strains: Strains were screened using silica-solubilizing medium solid plates. Burkholderia contaminans AHA-5 (CGMCC NO.: 30396), which could produce obvious silica-solubilizing transparent rings on silica-solubilizing medium solid plates, was selected for further study.

[0053] (2) Microbial culture: Burkholderia cepacia AHA-5 was cultured at 30°C and 200 rpm for 2-4 days in Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose or in nutrient-deficient Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose (the original component content of LB medium was 0.05-50%).

[0054] (3) Co-cultivation of rare earth elements in leaching waste FCC catalyst (the main framework is aluminosilicate, containing rare earth elements such as lanthanum and cerium, as well as transition elements such as nickel and vanadium, with a content of 0.01%-10%): Burkholderia cepacia AHA-5 is co-incubated with waste FCC catalyst at a liquid-solid ratio of 1:2-1:500 in Luria-Bertani (LB) liquid medium with 60 g / L glucose or in nutrient-deficient Luria-Bertani (LB) liquid medium with 60 g / L glucose (the original component content of LB medium is 0.05-50%). The culture is carried out at 30℃ and 200 rpm for 2-4 days.

[0055] (4) Co-cultivation and leaching of waste NdFeB sludge (main component is NdFeB (Nd2Fe) 14 B) Rare earth elements in magnets (containing 1%-30% lubricating oil): Burkholderia cepacia AHA-5 is co-incubated with waste NdFeB sludge at a liquid-to-solid ratio of 1:4-1:500 in Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose or in nutrient-deficient Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose (the original component content of LB medium is 0.05-50%). The culture is carried out at 30℃ and 200 rpm for 2-8 days.

[0056] (5) Co-cultivation and leaching of waste NdFeB powder (main component is NdFeB (Nd2Fe) 14B) Rare earth elements generated in various stages of industrial production of NdFeB magnets, such as cutting, molding, and sintering processes: Burkholderia cepacia AHA-5 is co-incubated with waste NdFeB sludge at a liquid-to-solid ratio of 1:4 to 1:500 in Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose or in nutrient-deficient Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose (the original component content of LB medium is 0.05-50%). The culture is carried out at 30°C and 200 rpm for 2-8 days.

[0057] (6) Preparation of microbial culture metabolites: Centrifuge the microbial culture medium from step (2) at 8000 rpm for 5 min and collect the supernatant.

[0058] (7) Leaching of rare earth elements from waste FCC catalyst (the main framework is aluminosilicate, containing rare earth elements such as lanthanum and cerium, as well as transition elements such as nickel and vanadium, with a content of 0.01%-10%) from microbial culture metabolites: Dilute the supernatant of the leachate in step (5) with ultrapure water (minimum supernatant content 2%), then mix the original solution or diluted solution with the waste FCC catalyst at a liquid-solid ratio of 1:2-1:500, and leach at 30℃ and 200rpm for 2-4 days to obtain the leachate.

[0059] (8) Leaching of waste NdFeB sludge from microbial culture metabolites (main component is NdFeB (Nd2Fe) 14 B) Rare earth elements in magnets containing 1%-30% lubricating oil: Dilute the supernatant of the leachate in step (5) with ultrapure water (minimum supernatant content 2%), then mix the original solution or diluted solution with waste NdFeB sludge at a liquid-to-solid ratio of 1:50, and leach at 30°C and 200 rpm for 2 days to obtain the leachate.

[0060] (9) Leaching of waste NdFeB powder from microbial culture metabolites (main component is NdFeB (Nd2Fe) 14 B), rare earth elements generated in various stages of industrial production of NdFeB magnets, such as cutting, molding, sintering, etc.: dilute the supernatant of the leachate in step (5) with ultrapure water (minimum supernatant content 2%), mix the original solution or diluted solution with waste NdFeB sintering or molding powder at a liquid-solid ratio of 1:50, leach at 30°C and 200 rpm, and obtain leachate after 2 days.

[0061] (10) The leaching capacity of AHA-5 was determined by detecting the content and type of rare earth elements in steps (3)(4)(5)(7)(8) using ICP-OES and ICP-MS.

[0062] The strain provided by this invention has low cultivation cost, fast growth rate, simple leaching conditions, and the ability to leach rare earth ions from various wastes. It has good application prospects in the microbial recycling and reuse of rare earth waste.

[0063] The raw materials and reagents used in the method for extracting rare earth elements from Burkholderia cepacia and its leaching waste provided by this invention are all commercially available.

[0064] The present invention will be further illustrated below with reference to the embodiments:

[0065] Example 1: Screening of rare earth leaching strains and determination of their ability to dissolve insoluble silicates

[0066] A single colony of purified Burkholderia contaminans AHA-5 was picked from LB solid medium containing 1.5-3% agar and added to 4 mL of LB liquid medium. The culture was incubated overnight at 30°C and 200 rpm in a constant temperature shaking incubator. 2 μL of the bacterial culture was then added to a silica-dissolving solid culture plate. After 10 days of incubation, the results were observed and recorded. The appearance of a clear ring was recorded as a positive result, indicating that the strain has the ability to dissolve insoluble silicates. The culture was then biologically preserved and the preservation number was CGMCC No. 30396.

[0067] Example 2: Rare earth elements in waste FCC catalyst leaching by AHA-5 microbial co-culture

[0068] A single colony of purified AHA-5 (CGMCC No. 30396) was picked from LB solid medium containing 1.5-3% agar and transferred to 4 mL of LB liquid medium. The culture was incubated overnight at 30°C and 200 rpm in a shaker until the OD value reached 0.8-1.2, serving as the seed culture. 1% (v / v) of the seed culture (final OD value 0.008-0.012) was inoculated into LB liquid medium containing 60 g / L glucose. This medium contained spent FCC catalyst (main framework of aluminosilicate, containing rare earth elements such as lanthanum and cerium, and transition elements such as nickel and vanadium, at a solid-liquid ratio of 1:2-1:500, with a content of 0.01%-10%). The culture was incubated at 30°C and 200 rpm in a shaker for 2-4 days. The supernatant was collected by centrifugation at 8000 rpm for 5 min.

[0069] After the solution becomes clear and transparent, dilute and bring to a final volume to achieve a target element content of 1 ppb-10 ppm. Then, perform quantitative analysis of rare earth elements using ICP-OES and ICP-MS (determined according to the national standard GB / T18115-2021, Chemical Analysis Methods for Rare Earth Impurities in Rare Earth Metals and Their Oxides).

[0070] Table 1

[0071] solid-liquid ratio Leaching rate 1:2 21.34％ 1:5 42.84％ 1:10 95.49％ 1:50 97.03％ 1:100 99.78％ 1:500 99.98％

[0072] Example 3: Rare earth elements in waste FCC catalyst leaching by AHA-5 microbial co-culture

[0073] A single colony of purified AHA-5 (CGMCC No. 30396) was picked from LB solid medium containing 1.5-3% agar and added to 4 mL of LB liquid medium. The culture was incubated overnight at 30°C and 200 rpm in a shaker until the OD value reached 0.8-1.2, serving as the seed culture. 1% (v / v) of the seed culture (final OD value 0.008-0.012) was inoculated into a nutrient-deficient Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose (the original LB medium composition was 0.05-50%). This medium contained spent FCC catalyst (main framework of aluminosilicate, containing rare earth elements such as lanthanum and cerium, and transition elements such as nickel and vanadium, at a liquid-to-solid ratio of 1:2-1:500, with a content of 0.01%-10%). The culture was incubated at 30°C and 200 rpm in a shaker for 2-4 days. The supernatant was collected by centrifugation at 8000 rpm for 5 min.

[0074] Example 4: AHA-5 co-cultivation leaching of rare earth elements from waste NdFeB sludge

[0075] A single colony of purified AHA-5 (CGMCC No. 30396) was picked from LB solid medium containing 1.5-3% agar and added to 4 mL of LB liquid medium. The culture was incubated overnight at 30°C and 200 rpm in a shaker until the OD value reached 0.8-1.2, serving as the seed culture. A 1% (v / v) portion of the seed culture (final OD value 0.008-0.012) was inoculated into LB liquid medium containing 60 g / L glucose. This medium also contained waste NdFeB sludge (mainly composed of Nd2Fe) with a liquid-to-solid ratio of 1:4-1:500. 14 B) Magnets containing 1%-30% lubricating oil. Incubate at 30℃ and 200rpm in a constant temperature shaking incubator for 2-8 days. Collect the supernatant by centrifugation at 8000rpm for 5 minutes.

[0076] Example 5: AHA-5 co-cultivation leaching of rare earth elements from waste NdFeB sludge

[0077] A single colony of purified AHA-5 (CGMCC No. 30396) was picked from LB solid medium containing 1.5-3% agar and added to 4 mL of LB liquid medium. The culture was incubated overnight at 30°C and 200 rpm in a shaker until the OD value reached 0.8-1.2, serving as the seed culture. A 1% (v / v) portion of the seed culture (final OD value 0.008-0.012) was inoculated into a nutrient-deficient Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose (the original LB medium composition was 0.05-50%) containing waste NdFeB sludge (mainly composed of Nd2Fe) at a liquid-to-solid ratio of 1:4-1:500. 14 B) Magnets containing 1%-30% lubricating oil. Incubate at 30℃ and 200rpm in a constant temperature shaking incubator for 2-8 days. Collect the supernatant by centrifugation at 8000rpm for 5 minutes.

[0078] Example 6: AHA-5 co-cultivation leaching of rare earth elements in waste NdFeB powder

[0079] A single colony of purified AHA-5 (CGMCC No. 30396) was picked from LB solid medium containing 1.5-3% agar and added to 4 mL of LB liquid medium. The culture was incubated overnight at 30°C and 200 rpm in a shaker until the OD value reached 0.8-1.2, serving as the seed culture. A 1% (v / v) portion of the seed culture (final OD value 0.008-0.012) was inoculated into LB liquid medium containing 60 g / L glucose. This medium also contained waste NdFeB powder (mainly composed of Nd2Fe) at a liquid-to-solid ratio of 1:4-1:500. 14 B), generated in various stages of industrial NdFeB magnet production, such as cutting, molding, and sintering processes. Incubate at 30℃ and 200rpm in a constant-temperature shaking incubator for 2-8 days. Collect the supernatant by centrifugation at 8000rpm for 5 minutes.

[0080] Example 7: AHA-5 co-culture leaching of rare earth elements in waste NdFeB powder

[0081] A single colony of purified AHA-5 (CGMCC No. 30396) was picked from LB solid medium containing 1.5-3% agar and added to 4 mL of LB liquid medium. The culture was incubated overnight at 30°C and 200 rpm in a shaker until the OD value reached 0.8-1.2, serving as the seed culture. A 1% (v / v) portion of the seed culture (final OD value 0.008-0.012) was inoculated into a nutrient-deficient Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose (the original LB medium composition was 0.05-50%) containing waste NdFeB powder (main component being Nd2Fe) at a liquid-to-solid ratio of 1:4-1:500.14 B), generated in various stages of industrial NdFeB magnet production, such as cutting, molding, and sintering processes. Incubate at 30℃ and 200rpm in a constant-temperature shaking incubator for 2-8 days. Collect the supernatant by centrifugation at 8000rpm for 5 minutes.

[0082] Example 8: Preparation of AHA-5 Microbial Extract

[0083] A single colony of purified AHA-5 (CGMCC No. 30396) was picked from LB solid medium containing 1.5-3% agar and added to 4 mL of LB liquid medium. The culture was incubated overnight at 30°C with a shaking incubator at 200 rpm until an OD value of 0.8-1.2 was reached, serving as the seed culture. A 1% (v / v) portion of the seed culture (final OD value 0.008-0.012) was inoculated into Luria-Bertani (LB) liquid medium supplemented with 60 g / L glucose and incubated at 30°C with a shaking incubator at 200 rpm until the logarithmic growth phase. The supernatant was collected by centrifugation at 8000 rpm for 5 min.

[0084] Example 9: Preparation of AHA-5 Microbial Extract

[0085] A single colony of purified AHA-5 (CGMCC No. 30396) was picked from LB solid medium containing 1.5-3% agar and added to 4 mL of LB liquid medium. The culture was incubated overnight at 30°C with a shaking incubator at 200 rpm until an OD value of 0.8-1.2 was reached, serving as the seed culture. A 1% (v / v) portion of the seed culture (final OD value 0.008-0.012) was inoculated into nutrient-deficient Luria-Bertani (LB) liquid medium (original LB medium composition 0.05-50%) and incubated at 30°C with a shaking incubator at 200 rpm until the logarithmic growth phase. The supernatant was collected by centrifugation at 8000 rpm for 5 min.

[0086] Example 10: Leaching rare earth elements from FCC using AHA-5 microbial culture supernatant

[0087] The original or diluted supernatant of the microbial culture from Example 8 (diluted with ultrapure water, with a minimum supernatant content of 2%) was added to the waste FCC catalyst (the main framework is aluminosilicate, containing rare earth elements such as lanthanum and cerium, as well as transition elements such as nickel and vanadium, with a content of 0.01%-10%), and mixed at a liquid-to-solid ratio of 1:2-1:500. The mixture was placed in a constant temperature shaking incubator at 30°C and 200 rpm, and the leachate was obtained after 2 days.

[0088] Example 11: Leaching rare earth elements from waste NdFeB sludge using AHA-5 microbial culture supernatant.

[0089] The original or diluted supernatant of the microbial culture from Example 8 (diluted with ultrapure water, minimum supernatant content 2%) was added to waste NdFeB sludge (the main component of which is NdFeB). 14 B) Mix the magnet (containing 1%-30% lubricating oil) with a liquid-solid ratio of 1:2-1:500, place it in a constant temperature shaking incubator at 30℃ and 200rpm, and obtain the leachate after 2 days.

[0090] Example 12: Leaching rare earth elements from waste NdFeB powder using AHA-5 microbial culture supernatant.

[0091] The original or diluted supernatant of the microbial culture from Example 8 (diluted with ultrapure water, minimum supernatant content 2%) was added to waste NdFeB powder (the main component of which is NdFeB (Nd2Fe)). 14 B), produced in various stages of industrial production of NdFeB magnets, such as cutting, molding, sintering, etc., is mixed in a liquid-to-solid ratio of 1:2-1:500 and placed in a constant temperature shaking incubator at 30℃ and 200rpm for 2 days to obtain the leachate.

[0092] Example 13: Leaching rare earth elements from FCC using AHA-5 microbial culture supernatant

[0093] The original or diluted supernatant of the microbial culture from Example 9 (diluted with ultrapure water, with a minimum supernatant content of 2%) was added to the waste FCC catalyst (the main framework is aluminosilicate, containing rare earth elements such as lanthanum and cerium, as well as transition elements such as nickel and vanadium, with a content of 0.01%-10%), and mixed at a liquid-to-solid ratio of 1:2-1:500. The mixture was placed in a constant temperature shaking incubator at 30°C and 200 rpm, and the leachate was obtained after 2 days.

[0094] Example 14: Leaching rare earth elements from waste NdFeB sludge using AHA-5 microbial culture supernatant.

[0095] The original or diluted supernatant of the microbial culture from Example 9 (diluted with ultrapure water, minimum supernatant content 2%) was added to waste NdFeB sludge (the main component of which is NdFeB). 14 B) Mix the magnet (containing 1%-30% lubricating oil) with a liquid-solid ratio of 1:2-1:500, place it in a constant temperature shaking incubator at 30℃ and 200rpm, and obtain the leachate after 2 days.

[0096] Example 15: Leaching rare earth elements from waste NdFeB powder using AHA-5 microbial culture supernatant.

[0097] The original or diluted supernatant of the microbial culture from Example 9 (diluted with ultrapure water, minimum supernatant content 2%) was added to waste NdFeB powder (the main component of which is NdFeB (Nd2Fe)). 14B), produced in various stages of industrial production of NdFeB magnets, such as cutting, molding, sintering, etc., is mixed in a liquid-to-solid ratio of 1:2-1:500 and placed in a constant temperature shaking incubator at 30℃ and 200rpm for 2 days to obtain the leachate.

[0098] Example 16: ICP-MS detection of the leaching capacity of AHA-5

[0099] The leachates from Examples 2-7 and 10-15 were filtered through a 0.22 μm needle filter membrane, and the filtrate was collected. Digestion was performed using a nitric acid-perchloric acid (10:1) mixed acid system on a 210°C hot plate. After the solution became clear and transparent, it was diluted to a final volume to achieve a target element concentration of 1 ppb-10 ppm. Quantitative analysis of rare earth elements was then performed using ICP-OES and ICP-MS (determined according to the national standard GB / T 18115-2021, Chemical Analysis Methods for Rare Earth Impurities in Rare Earth Metals and Their Oxides). The experimental results are shown in [Figure Number]. Figures 1-3 Tables 2-4.

[0100] Table 2. Leaching amount of rare earth elements from spent FCC catalyst by strain AHA-5

[0101]

[0102] Co-culture under normal conditions vs. microbial metabolites (Example 10) p = 0.005;

[0103] Co-culture under normal conditions vs. microbial metabolites (Example 13) p = 0.001;

[0104] Co-culture under normal conditions vs. co-culture under nutrient-deficient conditions: p<0.001;

[0105] Microbial metabolites (Example 10) vs. co-culture under nutrient-deficient conditions p = 0.450;

[0106] Microbial metabolites (Example 10) vs. microbial metabolites (Example 13) p = 0.959;

[0107] Microbial metabolites (Example 13) vs. co-culture under nutrient-deficient conditions p = 0.114.

[0108] As shown in Table 2:

[0109] The leaching rate of the co-culture group under normal conditions provided in Example 2 was 59.08%;

[0110] The leaching rate of the microbial metabolite group provided in Example 10 was 91.96%, which was significantly different from the co-culture group under normal conditions provided in Example 2 (P < 0.01).

[0111] The leaching rate of the microbial metabolite group provided in Example 13 was 93.72%, which was significantly different from the co-culture group under normal conditions provided in Example 2 (P < 0.01).

[0112] The leaching rate of the co-culture group under nutrient-deficient conditions provided in Example 3 was 97.03%, which was significantly different from that of the co-culture group under normal conditions provided in Example 2 (P < 0.01).

[0113] There was no significant difference between the nutrient-deficient co-culture group provided in Example 3 and the microbial metabolite group provided in Example 10 (P > 0.05).

[0114] There was no significant difference between the nutrient-deficient co-culture group provided in Example 3 and the microbial metabolite group provided in Example 13 (P > 0.05).

[0115] The leaching rate of the microbial metabolite group provided in Example 10 was 91.96%, and the leaching rate of the microbial metabolite group provided in Example 13 was 93.72%. There was no significant difference between the microbial metabolite group provided in Example 10 and the microbial metabolite group provided in Example 13 (P>0.05).

[0116] Table 3. Bar chart showing the leaching amount of rare earth elements from waste NdFeB sludge by strain AHA-5.

[0117]

[0118] Co-culture under normal conditions vs. microbial metabolites (Example 11) p = 0.032;

[0119] Co-culture under normal conditions vs. microbial metabolites (Example 14) p = 0.569;

[0120] Co-culturing under normal conditions vs. co-culturing under nutrient-deficient conditions: p = 0.041;

[0121] Microbial metabolites (Example 11) vs. co-culture under nutrient-deficient conditions p<0.001;

[0122] Microbial metabolites (Example 11) vs. microbial metabolites (Example 14) p = 0.015;

[0123] Microbial metabolites (Example 14) vs. co-culture under nutrient-deficient conditions p = 0.007.

[0124] As shown in Table 3:

[0125] The leaching rate of the co-culture group under normal conditions provided in Example 4 was 83.14%;

[0126] The leaching rate of the microbial metabolite group provided in Example 11 was 66.33%, which was significantly different from the co-culture group under normal conditions provided in Example 4 (P < 0.05).

[0127] The leaching rate of the microbial metabolite group provided in Example 14 was 80.35%. There was no significant difference between the microbial metabolite group provided in Example 14 and the co-culture group under normal conditions provided in Example 4 (P>0.05).

[0128] The leaching rate of the co-culture group under nutrient-deficient conditions provided in Example 5 was 96.00%, which was significantly different from the co-culture group under normal conditions provided in Example 4 (P < 0.05).

[0129] The co-culture group under nutrient-deficient conditions provided in Example 5 showed a highly significant difference compared to the microbial metabolite group provided in Example 11 (P < 0.01).

[0130] The co-culture group under nutrient-deficient conditions provided in Example 5 showed a highly significant difference compared to the microbial metabolite group provided in Example 14 (P < 0.01).

[0131] The leaching rate of the microbial metabolite group provided in Example 11 was 66.33%, while the leaching rate of the microbial metabolite group provided in Example 14 was 80.35%. The microbial metabolite group provided in Example 11 showed a significant difference compared with that provided in Example 14 (P < 0.05).

[0132] Table 4. Bar chart showing the leaching amount of rare earth elements from waste NdFeB powder by strain AHA-5.

[0133]

[0134] Co-culture under normal conditions vs. microbial metabolites (Example 12) p = 0.01;

[0135] Co-culture under normal conditions vs. microbial metabolites (Example 15) p<0.001;

[0136] Co-culture under normal conditions vs. co-culture under nutrient-deficient conditions: p<0.001;

[0137] Microbial metabolites (Example 12) vs. co-culture under nutrient-deficient conditions p = 0.003;

[0138] Microbial metabolites (Example 12) vs. microbial metabolites (Example 15) p<0.001;

[0139] Microbial metabolites (Example 14) vs. co-culture under nutrient-deficient conditions p = 0.15.

[0140] As shown in Table 4:

[0141] The leaching rate of the co-culture group under normal conditions provided in Example 6 was 56.80%;

[0142] The leaching rate of the microbial metabolite group provided in Example 12 was 69.44%, which was significantly different from that of the co-culture group under normal conditions provided in Example 6 (P < 0.05).

[0143] The leaching rate of the microbial metabolite group provided in Example 15 was 96.76%, which was significantly different from the co-culture group under normal conditions provided in Example 6 (P < 0.01).

[0144] The leaching rate of the co-culture group under nutrient-deficient conditions provided in Example 7 was 93.84%, which was significantly different from that of the co-culture group under normal conditions provided in Example 6 (P < 0.01).

[0145] The co-culture group under nutrient-deficient conditions provided in Example 7 showed a highly significant difference compared to the microbial metabolite group provided in Example 12 (P < 0.01).

[0146] The co-culture group under nutrient-deficient conditions provided in Example 7 showed a highly significant difference compared to the microbial metabolite group provided in Example 15 (P < 0.01).

[0147] The leaching rate of the microbial metabolite group provided in Example 12 was 69.44%, while the leaching rate of the microbial metabolite group provided in Example 15 was 96.76%. The microbial metabolite group provided in Example 12 showed a highly significant difference compared with the microbial metabolite group provided in Example 15 (P < 0.01).

[0148] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. Burkholderia contaminans AHA-5, characterized in that, Its accession number is CGMCC No.30396.

2. The method for culturing Burkholderia contaminans AHA-5 as described in claim 1, characterized in that, Burkholderia cepacia AHA-5 was selected and cultured for 2-4 days at 30°C and 200 rpm in Luria-Bertani liquid medium supplemented with 60 g / L glucose or in nutrient-deficient Luria-Bertani liquid medium supplemented with 60 g / L glucose. In the nutrient-deficient Luria-Bertani liquid medium, the original components of the LB medium account for 0.05-50%.

3. The method for preparing the metabolite of Burkholderia contaminans AHA-5 as described in claim 1, characterized in that, Burkholderia cepacia AHA-5 was selected and cultured in Luria-Bertani liquid medium supplemented with 60 g / L glucose or in nutrient-deficient Luria-Bertani liquid medium supplemented with 60 g / L glucose at 30°C and 200 rpm for 2-4 days. The culture medium was collected and centrifuged at 8000 rpm for 5 min, and the supernatant was collected. In the nutrient-deficient Luria-Bertani liquid medium, the original components of the LB medium account for 0.05-50%.

4. The application of Burkholderia contaminans AHA-5 as described in claim 1 in rare earth elements in leaching waste; wherein the waste includes one or more of waste FCC catalyst, waste NdFeB sludge, or waste NdFeB powder; The main framework of the spent FCC catalyst is aluminosilicate, containing rare earth elements and / or transition elements, with the content of rare earth elements and / or transition elements being 0.01% to 10%; the rare earth elements include lanthanum and / or cerium; the transition elements include nickel and / or vanadium. The main component of the waste NdFeB sludge is NdFeB (N2Fe). 14 B) Magnets containing 1% to 30% lubricating oil; The main component of the waste NdFeB powder is NdFeB (Nd2Fe). 14 B), which arises from various stages of industrial production of NdFeB magnets, including cutting, molding, or sintering processes.

5. A method for leaching rare earth elements from waste, characterized in that, Take the Burkholderia contaminans AHA-5 strain as described in claim 1 and mix it with the waste material, then incubate it. The waste includes one or more of the following: waste FCC catalyst, waste NdFeB sludge, or waste NdFeB powder; The main framework of the spent FCC catalyst is aluminosilicate, containing rare earth elements and / or transition elements, with the content of rare earth elements and / or transition elements being 0.01% to 10%; the rare earth elements include lanthanum and / or cerium; the transition elements include nickel and / or vanadium. The main component of the waste NdFeB sludge is NdFeB (N2Fe). 14 B) Magnets containing 1% to 30% lubricating oil; The main component of the waste NdFeB powder is NdFeB (Nd2Fe). 14 B), which arises from various stages of industrial production of NdFeB magnets, including cutting, molding, or sintering processes.

6. The method as described in claim 5, characterized in that, The liquid-to-solid ratio of Burkholderia contaminans AHA-5 to the spent FCC catalyst is 1:(2~500).

7. The method as described in claim 5, characterized in that, The liquid-to-solid ratio of the Burkholderia contaminans AHA-5 to the waste NdFeB sludge is 1:(4~500).

8. The method as described in claim 5, characterized in that, The liquid-to-solid ratio of the Burkholderia contaminans AHA-5 to the waste NdFeB powder is 1:(4~500).

9. The method as described in claim 5, characterized in that, The incubation conditions are 30°C and 200 rpm for 2-8 days.

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