Skin treatment device and skin treatment method

Abstract

The skin treatment device and the skin treatment method of the present invention are suitable for effectively suppressing melanin generation in the irradiated skin part of a person. A skin treatment device and a skin treatment method according to the present invention are provided with a light source that generates prescribed light having a central wavelength in a wavelength range of 490 nm to 525 nm or less, and are capable of irradiating the prescribed light from the light source to the skin. It is preferable that the prescribed light has a central wavelength of approximately 505 nm.

Description

Technical Field

[0001] The present invention relates to a skin treatment device and a skin treatment method. Background Art

[0002] There is known a skin wound healing device including an ultra-narrow-band light irradiation unit that generates ultra-narrow-band green light having a peak wavelength region of 500 nm to 540 nm and a half-value width of 10 nm or less.

[0003] Prior art literature

[0004] Patent Literature

[0005] Patent Document 1: WO2011 / 067941 Pamphlet Summary of the invention

[0006] Problems to be solved by the invention

[0007] However, in the above-mentioned conventional techniques, it is difficult to effectively suppress the production of melanin in the skin area of ​​a person to be irradiated.

[0008] Therefore, an object of the present invention is to effectively suppress melanin production in an irradiated human skin area.

[0009] Means for solving problems

[0010] In one aspect, a skin treatment device is disclosed, comprising: a light source that generates predetermined light having a central wavelength in a wavelength range longer than 490 nm and not more than 525 nm, and can irradiate the predetermined light from the light source onto skin.

[0011] Effects of the Invention

[0012] According to the present invention, melanin production in the irradiated skin area of ​​a person can be effectively suppressed. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a perspective view showing the appearance of the light irradiation device according to the present embodiment.

[0014] Figure 2 This is a diagram for explaining the characteristics of light (predetermined light) generated from an LED.

[0015] Figure 3 This is a diagram schematically showing a control system of the light irradiation device.

[0016] Figure 3A This is a diagram showing an example of the hardware configuration of the control device.

[0017] Figure 4AThis is a diagram showing the test results (part 1) of the melanin production inhibitory effect by green LED light irradiation.

[0018] Figure 4B This is a diagram showing the test results (part 2) of the melanin production inhibitory effect by green LED light irradiation.

[0019] Figure 5A This is a diagram showing the test results (part 3) of the melanin production inhibitory effect by green LED light irradiation.

[0020] Figure 5B This is a graph showing the test results (part 4) of the melanin production inhibitory effect by green LED light irradiation.

[0021] Figure 5C This is a graph showing the test results (part 5) of the melanin production inhibitory effect by green LED light irradiation.

[0022] Figure 5D This is a graph showing the test results (part 6) of the melanin production inhibitory effect by green LED light irradiation.

[0023] Figure 5E is from FIG. 5A to FIG. 5E Table of evaluation results obtained from the test results.

[0024] Fig. 6A This is a graph showing the test results (part 7) of the melanin production inhibitory effect by green LED light irradiation.

[0025] Figure 6B This is a graph showing the test results (part 8) of the melanin production inhibitory effect by green LED light irradiation.

[0026] Fig. 7A This is a graph showing the test results (part 9) of the melanin production inhibitory effect by green LED light irradiation.

[0027] Figure 7B This is a graph showing the test results (part 10) of the melanin production inhibitory effect by green LED light irradiation.

[0028] Figure 8 This is a table showing the relationship between the radiation intensity of green LED light and the effect (the effect of suppressing melanin production).

[0029] Fig. 9 This is a graph showing the relationship between the radiation intensity of green LED light and the effect (Part 1).

[0030] Fig.10 This is a graph showing the relationship between the radiation intensity of green LED light and the effect (part 2).

[0031] Fig.11 This is a graph showing the relationship between the radiation intensity of green LED light and the effect (part 3).

[0032] Fig.11A This is a graph showing other test results related to the relationship between the radiation intensity and effect of green LED light.

[0033] Fig.12 This is a graph showing the test results (part 1) of the difference in the melanin production inhibitory effect caused by the difference in wavelength.

[0034] Fig.13 This is a graph showing the test results (part 2) of the difference in the melanin production inhibitory effect caused by the difference in wavelength.

[0035] Fig.14 This is a graph showing the test results (part 3) of the difference in the melanin production inhibitory effect caused by the difference in wavelength.

[0036] Fig.15 This is a graph showing the test results related to the expression level of keratin 10. DETAILED DESCRIPTION

[0037] Hereinafter, each embodiment will be described in detail with reference to the drawings.

[0038] Figure 1 It is a perspective view showing the light irradiation device 1 of this embodiment.

[0039] The light irradiation device 1 generates light that can be irradiated to human skin. The light may also have a beauty-related effect. In this case, the beauty-related effect is arbitrary and may include hair removal, skin beautification, elimination of sagging, tightening, fat burning, lifting, face reduction, improvement of skin elasticity, luster, moisturizing, or any combination of more than one of them. In addition, the beauty-related effect may be an effect that can be quantified or an effect that cannot be quantified.

[0040] The functions of the light irradiation device 1 can be realized by the computer in the light irradiation device 1 alone, or by the combination of the computer in the light irradiation device 1 and an external server and / or a user terminal. In addition, in this case, the computer reading program that realizes the functions of the light irradiation device 1 can be executed by the computer in the light irradiation device 1, or by the combination of the computer in the light irradiation device 1 and an external server and / or a user terminal.

[0041] also, Figure 1 The light irradiation device 1 shown is a portable device that can be held by a user's hand, but can also be applied to a movable type that is movably supported by a fixed device via an arm or the like.

[0042] The light irradiation device 1 includes a grip portion 2 and a head 3. In this case, the user can locally irradiate the desired part with light from the light irradiation device 1 by gripping the grip portion 2 and directing a skin-facing portion 3a (described later) of the head 3 toward a desired part of the user's face or body.

[0043] The grip portion 2 has a shape that is easy to be gripped by the user's hand. The grip portion 2 may include: an input portion (not shown), which includes various buttons such as a power on / off button, a mode switching button, and an intensity adjustment button. In addition, the various buttons may be mechanical buttons or touch switches. In addition, a display portion (not shown) that displays the state of the light irradiation device 1 may also be provided on the grip portion 2.

[0044] The head 3 is provided at the end of the grip 2. The head 3 may be fixed to the grip 2, may be detachable, or may be movable relative to the grip 2. In addition, a plurality of detachable attachments may be provided.

[0045] The head 3 may have a skin-relative portion 3a that is roughly flat or curved (a curved surface with a relatively large radius of curvature). The shape of the skin-relative portion 3a when observed from the front (the shape when observed in a direction perpendicular to the skin-relative portion 3a) is any shape such as a rectangle, a circle, an ellipse, a polygon, etc. The skin-relative portion 3a may be formed of any material through which light can pass, such as glass. In the present embodiment, the skin-relative portion 3a is formed by a light emitting surface. In addition, the skin-relative portion 3a is in a shape in which the light emitting surface is exposed, but it may also be covered by a lens, etc. Preferably, the skin-relative portion 3a is in a concave shape that is concave relative to the skin in the up and down directions. In this case, cosmetics containing an object that is a penetration object facing the skin can be contained in the concave portion, and skin treatment based on light irradiation can be performed, which can improve the skin treatment effect.

[0046] A light irradiation unit 30 is embedded in the head 3 (in Figure 1 Not shown in the figure, refer to Figure 3 ). The light irradiation unit 30 generates light to be irradiated to the skin via the skin-facing portion 3a. That is, the light irradiation unit 30 irradiates light to the skin via the skin-facing portion 3a. In addition, an optical system such as a light guide may be arranged between the light irradiation unit 30 and the skin-facing portion 3a, or an optical system may not be arranged.

[0047] In this embodiment, the light irradiation unit 30 includes an LED (Light Emitting Diode) 36. Only one LED 36 may be provided, or a plurality of LEDs 36 may be provided. When a plurality of LEDs 36 are provided, the plurality of LEDs 36 may be provided, for example, in a plane substantially parallel to the skin-facing portion 3a.

[0048] In addition, in the present embodiment, the light irradiation device 1 is portable, but it can also be a fixed type, and the form is arbitrary. In addition, the light irradiation device 1 can also be in the form of a mask worn on the face, or in a form that can be wrapped around the body for use. In addition, the irradiation range of the light irradiation device 1 is also arbitrary, and the skin can be irradiated in various ways such as a wide range irradiation method and a point irradiation method. In addition, the light irradiation device 1 has a head 3 that is different from the light irradiation unit 30, but it can also be a structure in which the head 3 and the light irradiation unit 30 are integrated.

[0049] Figure 2 This is a diagram for explaining the characteristics of light (predetermined light) generated from the LED 36 , with the wavelength being the horizontal axis and the intensity being the vertical axis, and shows an example of the characteristics.

[0050] Preferably, LED 36 is a light source that generates a prescribed light having a central wavelength in a wavelength range longer than 490 nm and less than 525 nm. The basis (technical significance) of the superiority of this wavelength range (and the wavelength of approximately 505 nm therein) will be referred to Figure 4A , Figure 4B ,as well as Fig.12 In addition, 505 nm and its vicinity are strictly blue-green wavelengths, and 525 nm and its vicinity are strictly green wavelengths, but the wavelength range longer than 490 nm and below 525 nm may be expressed as "green" below.

[0051] like Figure 2 As shown, more preferably, the prescribed light has a central wavelength of approximately 505 nm. If the central wavelength is such, melanin production in the irradiated user's skin portion can be suppressed as described later compared to a case where such a central wavelength is not such. For example, the prescribed light can be irradiated in a manner having a good melanin production suppression effect of 5% or more, more preferably 10% or more, in the irradiated user's skin portion compared to a case where the prescribed light is not irradiated.

[0052] Preferably, the half-value half-width of the light is specified (see Figure 2 ) is less than ±20nm, more preferably about ±10nm. Thus, the inhibitory effect of melanin production in the irradiated user's skin area can be maximized.

[0053] The light irradiation unit 30 preferably emits 0.5 mW / cm 2 Above and 62mW / cm 2 The radiation intensity is in the range of 11.5 mW / cm 2 Above 30mW / cm 2 The following range of radiation intensity is used to irradiate the specified light. For relevant test results, refer to Figure 8 The following figures will be described later.

[0054] In addition, the light irradiation unit 30 preferably provides 0.09 J / cm 2 Above and 30J / cm 2 The irradiation energy is in the range of 0.09 J / cm 2 Above and 11J / cm 2 The prescribed light is irradiated at the irradiation energy in the following range.

[0055] In addition, it is also possible to mount multiple LEDs 36 on one chip. Alternatively, LED 36 may be combined with other LEDs having other central wavelengths to form a single chip. For example, when LED 36 and a red LED are formed into a single chip, the ratio of the number of green LEDs and red LEDs in one chip may be appropriately matched.

[0056] Figure 3 It is a diagram schematically showing a control system of the light irradiation device 1 .

[0057] The light irradiation device 1 includes a control device 100, and a power supply 90 and an LED 36 are electrically connected to the control device 100. The control device 100 operates based on the power from the power supply 90 and controls the LED 36. The LED 36 operates based on the power from the power supply 90 under the control of the control device 100. The power supply 90 may include an external power supply and / or an internal power supply. In addition, the internal power supply may be a rechargeable battery.

[0058] The control device 100 irradiates the skin with the prescribed light via the LED 36. At this time, the control device 100 can realize continuous light irradiation for more than 1 minute, with the irradiation time of the prescribed light accounting for more than 1 / 2. In this case, light of other wavelengths may be irradiated during the time other than the irradiation time of the prescribed light, or no light may be irradiated (that is, the irradiation of the prescribed light may be intermittent).

[0059] In addition, the control device 100 can control the irradiation of light from the head 3 in one or more action modes. The one or more action modes may include a prescribed action mode corresponding to the melanin production inhibitory effect, or a prescribed action mode corresponding to an effect related to the melanin production inhibitory effect. In this case, the control device 100 outputs prescribed light from the head 3 in the prescribed action mode.

[0060] Figure 3A 1 is a diagram showing an example of the hardware configuration of the control device 100. Figure 3A , the control object 60 is schematically illustrated in association with the hardware configuration of the control device 100 .

[0061] The control device 100 includes a CPU (Central Processing Unit) 11, a RAM (Random Access Memory) 12, a ROM (Read Only Memory) 13, an auxiliary storage device 14, a drive device 15 and a communication interface 17 connected via a bus 19, and a wired transceiver 25 and a wireless transceiver 26 connected to the communication interface 17.

[0062] The auxiliary storage device 14 is, for example, a HDD (Hard Disk Drive) or an SSD (Solid State Drive), and is a storage device that stores data related to application software and the like.

[0063] The wired transceiver 25 includes a transceiver capable of communicating using a wired network. The wired transceiver 25 is connected to a control object 60. Part or all of the control object 60 may be connected to the bus 19 or to the wireless transceiver 26.

[0064] The wireless transceiver 26 is a transceiver that can communicate using a wireless network. The wireless network may include a wireless communication network of a mobile phone, the Internet, a VPN (Virtual Private Network), a WAN (Wide Area Network), etc. In addition, the wireless transceiver 26 may also include a near field communication (NFC: Near Field Communication) unit, a Bluetooth (Bluetooth, registered trademark) communication unit, a Wi-Fi (Wireless-Fidelity) transceiver unit, an infrared transceiver unit, etc. In addition, the control device 100 may also communicate with a server (not shown) via the wireless transceiver 26 to obtain various information.

[0065] In addition, the control device 100 may also be connected to a recording medium 16. The recording medium 16 stores a predetermined program. The program stored in the recording medium 16 is installed in the auxiliary storage device 14 of the control device 100 via the drive device 15. The installed predetermined program can be executed by the CPU 11 of the control device 100. For example, the recording medium 16 may be a recording medium that records information optically, electrically or magnetically, such as a CD (Compact Disc)-ROM, a floppy disk, a magneto-optical disk, etc., a semiconductor memory that records information electrically, such as a ROM, a flash memory, etc. In addition, the recording medium 16 does not contain a carrier wave.

[0066] Next, the effect of the above-mentioned predetermined light (the effect of suppressing melanin production) will be further described with reference to test results and the like.

[0067] Since visible light is less invasive to organisms, various studies have been conducted on the effects of visible light on organisms for the purpose of applying it to the medical field and the field of skin beauty (Imagawa et al., Japanese Journal of Physicians, 32, 444 (2012)). For example, there are also reports that red light plays an important role in preventing skin aging, and green and yellow light play an important role in inhibiting excessive cell activity.

[0068] Melanin, which is the cause of pigmentation in the skin, plays an important role in preventing DNA from being damaged by harmful ultraviolet rays generated in melanocytes, but on the other hand, it is also the cause of spots, and there is a high demand for improvement. Therefore, green LED light that inhibits cell activity also affects the activity of melanoma, and whether it can reduce the amount of melanin produced was verified.

[0069] The inventors of this application commissioned the following test to Toin Yokohama University to verify the melanin production inhibitory effect of green LED. The melanin production inhibitory effect was verified using mouse-derived B164A5 cells (B16 melanoma cells, RIKEN BRC) and human-derived melanoma cells (HMV-II cells, Keisei) obtained from the university. In addition, the light sources of green LEDs used USHIO Electron SMT525 (wavelength 525nm) and SMT505 (wavelength 505nm).

[0070] (Cell survival count of B16 melanoma cells)

[0071] B16 melanoma cells were expressed at 1×10 4 and 2×10 4 Cells / mL were inoculated into a 6-well plate, cultured at 37°C and 5% CO2 for 3 days, and then replaced with a culture medium without phenol red. Green LED irradiation was performed once a day for 3 days, and then cultured for 1 day. Then, Cell Counting Kit-8 (manufactured by Tongren Chemical Co., Ltd.) was added and cultured for 3 hours. After culture, the culture medium was dispensed, the absorbance at 450nm was measured, and the number of viable cells was calculated. The greater the absorbance at 450nm, the greater the number of viable cells.

[0072] (Evaluation of melanin production inhibition in B16 melanoma cells)

[0073] B16 melanoma cells were expressed at 1×10 4 and 2×10 4Cells / mL were seeded in a 6-well plate, cultured at 37°C and 5% CO2 for 3 days, and then replaced with a phenol red-free medium containing 100nM of the melanin synthesis inducer α-MSH. The green LED was irradiated once a day for 3 days, and after 1 day of culture, the cells were washed with PBS(-)1mL, and the cells were dissolved with a 2mol / L sodium hydroxide aqueous solution containing 10wt% dimethyl sulfoxide (DMSO), and the melanin production was measured by the absorbance at 405nm. In addition, the amount of cell-derived protein was measured using the RC DCTM protein assay (manufactured by BioRad). Based on the measurement results, the amount of melanin per unit of cell-derived protein was calculated.

[0074] (Melanin production inhibition effect caused by green LED light irradiation)

[0075] It was confirmed that when irradiated with green LED light of wavelengths of 505nm and 525nm, the number of B16 melanoma cells that survived decreased and the amount of melanin produced decreased. This tendency was more significant in the case of green LED light of 505nm. Figure 4A and Figure 4B The test results shown are clear. Figure 4A This is a graph showing the number of surviving B16 melanoma cells when irradiated with green LED light having a wavelength of 505 nm. Figure 4B This is a graph showing the number of B16 melanoma cells that survived when irradiated with green LED light of 525 nm wavelength. In this experiment, the initial cell concentration was 1×10 4 (Cells / mL) and 2×10 4 When the concentration of 50 μg / mL was 100 μg / mL, the concentration after 30 minutes and after 60 minutes was measured.

[0076] (Evaluation of the inhibition of melanin production by HMV-II derived from human melanoma cells)

[0077] It has been reported that mouse-derived B16 melanoma cells and human-derived HMV-II melanoma cells have different sensitivities to whitening agents. In the above evaluation of B16 cells, the inhibitory effect of 505nm green LED light on melanin production was more significant, so the wavelength was narrowed to 505nm in HMV-II cells to evaluate its effect.

[0078] Unlike B16 cells, the melanoma cell proliferation effect of adding the melanin synthesis inducer α-MSH to HMV-II cells was low. Therefore, in the evaluation of HMV-II cells, theophylline, which is an enhancer of MSH, was used as a melanin synthesis inducer.

[0079] 1×10 4Cells / mL of HMV-II cell dispersion was dispensed into 66-well plates at a rate of 1×10 4 Cells / mL were inoculated, cultured at 37°C, 5% CO2 for 3 days, and then replaced with 2mL of phenol red-free medium containing 25μL of Theophylline after culturing at 40mM. 505nm LED light was irradiated once a day, and after 3 days, cultured for 1 day, 2M NaOH solution containing 10wt% dimethyl sulfoxide (DMSO) was used to dissolve the cells with 2mol / L sodium hydroxide aqueous solution, and the melanin production was measured based on the absorbance at 405nm. In addition, the amount of cell-derived protein was measured using RC DCTM protein assay (manufactured by BioRad). Based on the measurement results, the amount of melanin per unit of cell-derived protein was calculated.

[0080] (Survival rate of HMV-II cells)

[0081] HMV-II cells were cultured at 1×10 4 Cells / mL were inoculated into a 6-well plate, cultured at 37°C and 5% CO2 for 3 days, and then replaced with a culture medium without phenol red. 505nm LED irradiation was performed once a day, and after 3 days, cultured for 1 day. Then, CellCounting Kit-8 (manufactured by Tongren Chemical Co., Ltd.) was added and cultured for 3 hours. After culture, the culture medium was dispensed and the absorbance at 450nm was measured. After the measurement, the survival rate was calculated as a relative value to the absorbance at 450nm without LED light irradiation.

[0082] (Melanin production inhibition effect caused by green LED light irradiation)

[0083] It was confirmed that the survival rate of HMV-II cells decreased and the amount of melanin produced decreased when irradiated with 505 nm LED light.

[0084] It was verified that green LED light irradiation is effective in inhibiting melanin production in both B16 cells and HMV-II cells. FIG. 5A to FIG. 5D Other test results are shown. FIG. 5A to FIG. 5D The other tests shown are also consistent with the reference Figure 4A and Figure 4B The above-mentioned test was carried out in the same manner. In addition, Figure 5E is from FIG. 5A to FIG. 5D The table of evaluation results obtained from the test results of . It can be seen from these that the melanin production inhibitory effect of 505nm is higher than that of 525nm, and 20 minutes is more significant than 10 minutes. In addition, inhibition was also observed at 30 minutes and 60 minutes for 505nm, but no inhibitory effect was observed at 30 minutes for 525nm, but it was inhibited at 60 minutes.

[0085] This example confirmed the effect of suppressing the production of melanin, which is the cause of spots, under green LED light, especially at a wavelength of 505nm. FIG. 6A to FIG. 7B The test results shown are clear. Fig. 6A and Figure 6B Represent the results of the first and second tests, respectively. Fig. 7A and Figure 7B Represent the results of the first and second tests respectively. FIG. 6A to FIG. 7B The other tests shown are also consistent with the reference Figure 4A and Figure 4B The above-mentioned experiments were carried out in the same manner.

[0086] Figures 8 to 11 is a graph showing other test results. Figure 8 This is a table showing the relationship between the radiation intensity of green LED light and the effect (the effect of inhibiting melanin production). Figures 9 to 11 This is a graph showing the relationship between the radiation intensity of green LED light and the effect. Fig.11A This is a graph showing other test results related to the relationship between the radiation intensity and effect of green LED light.

[0087] Figures 8 to 11 The other experiments shown were performed as follows.

[0088] B16 melanoma 4A5 used the substance provided by RIKEN BRC. In the content of this experiment, the term "cell" shown below refers to this point. The cells and cell culture described in the following steps S1 to S3 were performed, and the following culture medium was used as the culture medium.

[0089] Dulbecco's Modified Eagle Medium (DMEM, Cat No. 10566-016, Gibco, USA) containing 10.0% (v / v) Fetal Bovine Serum (FBS, Cat No. SH30071.03, Hyclone (registered trademark), UK) and 1.0% (v / v) antifungal agent (Antibiotic-Antimycotic 100X, Cat No. 15240-062, Invitrogen, USA) was used. The culture medium was prepared by mixing DMEM containing 10% FBS and 100 nM α-Melanocyte stimulating hormone (α-MSH, Cat No. M4135, Sigma-Aldrich, USA) and 100 μM theophylline (Cat No. T1633, Sigma-Aldrich, USA).

[0090] Step S1: Cell culture and passaging

[0091] In a 60 mm dish (Cat No. 353002, Falcon (registered trademark), USA), 3.0 × 10 5 The cells were inoculated at a density of 10 cells / dish and cultured in a CO2 incubator (CO2 concentration = 5%, 37°C) for 24 hours.

[0092] Step S2: Irradiate green LED light

[0093] The culture medium was removed, washed with phosphate buffer saline (PBS (-), Cat No. 198601, Nissui, Japan), and then replaced with 8 mL of Hanks balanced salt solution (+) (HBSS (+), Cat No. 084-08965, Wako, Japan), and then the irradiation equipment was applied according to the irradiation conditions. HBSS (+) was further removed and replaced with 3 mL of test culture medium, and cultured for 72 hours. In addition, regarding the irradiation conditions, the irradiation time was set to 3 minutes.

[0094] Step S3: After culturing, wash with PBS, then dilute alamarBlue (registered trademark) (Cat No. DAL1100, Invitrogen (registered trademark), USA) 10 times with serum-free DMEM, treat the cells with 2 mL of the obtained alamarBlue solution, and culture at 37°C in a CO2 incubator for 2 hours. Recover the alamarBlue solution, add 200 μL to a 96-well plate (Cat No. 9017, costar, USA), and use a microplate reader (SPARK (registered trademark) 10M, TECAN, Switzerland) to measure the absorbance (OD570, OD600) at 570nm and 600nm. Use the alamarBlue solution as a blank. Remove the alamarBlue solution from the 60mm dish, wash with PBS (-), and in order to solubilize the melanin, add 1 mL of a 1M sodium hydroxide aqueous solution containing 10% DMSO and incubate at 85°C for 10 minutes. Add 100 μL of melanin dissolution solution to a 96-well plate, and use a microplate reader to measure the absorbance at 405 nm (OD405). Set the OD570-600 of the control to 100%, and calculate the cell survival rate of the LED application group. In addition, set the OD405 of the control to 100%, and calculate the melanin production rate. Moreover, the OD405 of the control and LED irradiation application groups is divided by the OD570-600 measured with alamarBlue to calculate the melanin production rate per unit cell. The significance difference test is performed on the control and LED application groups using an uncorresponding t-test. The significance level is set to less than 5% on both sides of the test.

[0095] Depend on Figures 8 to 11 It can be seen that when B16 melanoma cells were irradiated with green LED (505 nm), the melanin production rate showed significantly lower values ​​than the control at any output, confirming that melanin production was inhibited. 2 ~11.5mW / cm 2 In the highest output of 11.5mW / cm 2 In the application, the melanin production rate per unit cell also showed a significantly lower value compared to the control, and the highest inhibitory tendency with production ability was output under this condition. Therefore, as described above, the radiation intensity of the prescribed light from the light irradiation unit 30 is preferably 0.5 mW / cm 2 The range is more preferably 11.5 mW / cm 2 above.

[0096] in addition, Fig.11AThe figure shows the difference in the effect when irradiating green LED light with a wavelength of 520nm with different radiation intensities. Fig.11A The test shown is also based on Figure 8 The same method is used to implement it. Fig.11A Here, for comparison, 9mW / cm 2 and 62W / cm 2 Graphs A, B, and C correspond to the cell survival rate, melanin production rate, and melanin production rate per unit cell, respectively.

[0097] from Fig.11A It is known that if the radiation intensity of the predetermined light from the light irradiation unit 30 is higher than a certain level, the effect will not be significantly increased (i.e., saturated). Therefore, as described above, the radiation intensity of the predetermined light from the light irradiation unit 30 is preferably 0.5 mW / cm 2 Above and 62mW / cm 2 The following range, more preferably 11.5 mW / cm 2 Above and 62mW / cm 2 In addition, from the perspective of power consumption, it can also be said that it is preferable to set the 2 The upper limit value is around 62mW / cm 2 .

[0098] In addition, Figures 8 to 11A In the test shown, the irradiation energy of the predetermined light from the light irradiation unit 30 is 0.09 J / cm 2 Above and 11J / cm 2 In addition, other test results disclosed in this specification also show that the irradiation energy of the predetermined light from the light irradiation unit 30 is 0.09 J / cm 2 Above and 45J / cm 2 Furthermore, even at a relatively low irradiation energy of 0.09 J / cm 2 Above and 11J / cm 2 Effective effects were also confirmed within the following ranges.

[0099] In addition, the light irradiation device 1 can irradiate the above-mentioned green LED light to the dry skin of the user, but can also irradiate the green LED light to the skin to which a gel or liquid containing a penetration object facing the skin is applied. In this case, the object of the penetration object is arbitrary. The object is a substance that can be applied to human skin, and typically, it can be a substance that can expect various effects such as beauty effects.

[0100] Next, refer to Fig.12The following drawings further illustrate the preferred wavelength range of light irradiated onto the skin.

[0101] In reference to the above Figure 4A and Figure 4B The above-mentioned experiment showed that when irradiating green LED light with wavelengths of 505nm and 525nm, the number of viable B16 melanoma cells decreased and the amount of melanin produced decreased.

[0102] Fig.12 2 is a graph showing other test results, showing the test results when irradiating with LED light of wavelengths of 450nm, 520nm and 850nm (near infrared wavelength). Fig.12 In the experiment, the cell survival rate, melanin production rate and melanin production rate per unit cell are shown when the wavelength of LED 36 is 450nm, 520nm and 850nm. Fig.12 In the figure, the comparison shows the test results without any irradiation.

[0103] also, Fig.12 The test results shown are compared with those mentioned above. Figure 4A and Figure 4B The above tests were carried out by different agencies, but the test methods were essentially the same.

[0104] Depend on Fig.12 It can be seen that melanin production inhibition is preferentially confirmed at wavelengths of 520nm and 450nm. That is, it is shown that the number of survival of B16 melanoma cells decreases and the amount of melanin produced decreases. In addition, it can be seen that the melanin production inhibition effect of 520nm may be improved compared with the wavelength of 450nm. In addition, some inhibition trends were observed at a wavelength of 850nm, but there was no significant difference, and the result was the lowest.

[0105] Fig.13 : is a graph showing another test result, which is a graph showing the difference in the melanin production inhibitory effect caused by the difference in three wavelengths. Fig.13 In the figure, the melanin production rate per unit cell is shown, and the test results are shown when the wavelengths of LED 36 are 505nm, 525nm and 630nm (red wavelength). Fig.13 In the example, the comparison also shows the results of a test without any irradiation.

[0106] also, Fig.13 The test results shown are compared with those mentioned above. Figure 4A and Figure 4B The above tests were carried out by different agencies, but the test methods were essentially the same.

[0107] Depend on Fig.13It is found that at wavelengths of 525 nm and 630 nm, no significant suppression of melanin production is observed as at a wavelength of 505 nm.

[0108] Fig.14 : is a graph showing another test result, which is a graph showing the difference in the melanin production inhibitory effect caused by the difference in four wavelengths. Fig.14 In the figure, the cell survival rate is shown as the test results when the wavelength of LED 36 is 470nm (blue wavelength), 490nm, 505nm and 525nm. Fig.14 In the figure, the comparison also shows the test results without any irradiation.

[0109] also, Fig.14 The test results shown are compared with those mentioned above. Figure 4A and Figure 4B The above tests were carried out by different agencies, but the test methods were essentially the same.

[0110] Depend on Fig.14 It can be seen that the survival rate of B16 melanoma cells is lower at wavelengths of 470 nm, 505 nm, and 525 nm than at a wavelength of 490 nm, that is, the superiority of wavelengths of 470 nm, 505 nm, and 525 nm to a wavelength of 490 nm (superiority of the melanin production inhibitory effect) is shown.

[0111] Fig.15 : is a graph showing the test results on the expression level of keratin 10. Fig.15 , regarding the expression level of keratin 10, test results when the wavelengths of LED 36 were irradiated at 470 nm, 505 nm, and 590 nm (yellow wavelength), respectively, are shown.

[0112] An outline of the test method is as follows.

[0113] (Step S1) Cell pre-culture

[0114] Human epidermal keratinocytes (NHEK) were dormant in a T-75 flask using culture medium and cultured in a CO2 incubator (5% CO2, 37°C, humidified). When the cells reached about 80% confluence, they were passaged in a T-225 flask and cultured until the necessary number of cells was obtained before use in subsequent experiments. The cell passage method is as follows: After washing the cells with PBS (- / -), 0.05% Trypsin-EDTA was used to peel off the cells, and trypsin neutralizing solution was added to neutralize the trypsin. Next, the cell suspension was recovered into a centrifuge tube and centrifuged (room temperature, 180xg, 5min). The supernatant was removed, and the culture medium was added again to suspend the cells, and the number of cells was counted. The cells were suspended to the target cell density using culture medium and inoculated into the culture vessel used in the experiment.

[0115] (Step S2) Cell Treatment

[0116] The cells were inoculated in a 60 mm dish at 900,000 cells / dish / 3 mL. On the second day of inoculation, 5 mL of culture medium was added, making the total 8 mL, and the machine treatment was performed for 30 seconds. The treatment was performed every 24 hours ± 1 hour, and the beauty device treatment was performed 3 times in total. In the first machine treatment, the culture medium was set to 8 mL and calcium chloride was added. Then, the treatment was performed for 72 hours.

[0117] (Step S3) RNA extraction

[0118] Using cells 24 hours after the final machine treatment, RNA was extracted using the RNeasy Plus Mini Kit. The method followed the kit protocol. After RNA extraction, the concentration was measured using NanoDrop Eight and stored at -80°C.

[0119] (Step S4) Quantitative PCR

[0120] One Step real-time RT-PCR was performed using the QuantiTect Probe RT-PCR Kit attached to the Fast Lane Cell Probe Kit with the composition in the table below. The RNA sample was diluted to about 100 ng / μL before use. The RT-PCR reaction was performed under the conditions of 50°C, 30 min-95°C, 15 min-(94°C, 15 sec-60°C, 60 sec) × 40 cycles. The GAPDH gene was used as the internal standard gene.

[0121] Depend on Fig.15It can be seen that the expression amount of keratin 10 is significantly increased at a wavelength of 505 nm compared to wavelengths of 470 nm and 590 nm, that is, the superiority of the wavelength of 505 nm over the wavelengths of 470 nm and 590 nm (the superiority of the expression effect of keratin 10) is shown.

[0122] Here, the mechanism of metabolism is explained. The epidermis is arranged in four layers: basal layer, spinous layer, granular layer, and stratum corneum. Metabolism at the cellular level is carried out in four stages: cell proliferation in the basal layer, synthesis of keratin (K10) in the spinous layer, cell death (apoptosis and necrosis) in the granular layer, and cutting of the protease (KLK8) in the stratum corneum. In human skin, the process of generating epidermal cells in the basal layer, maturing while moving to the upper layer, and peeling off the stratum corneum is called metabolism.

[0123] If the synthesis of keratin decreases, the structure of the epidermis cannot be maintained, resulting in wrinkles and sagging. In rapid metabolism, immature epidermal cells are exposed to the outside world during the period when they are not keratinized. In addition, excessive metabolism can cause the pathological state of atopic dermatitis. In slow metabolism, keratin that should be peeled off as dirt will remain and become thick. Furthermore, abnormal delays can cause psoriasis, keratosis, etc. Therefore, indicators other than cell proliferation are also essential factors based on considering normal metabolism.

[0124] Thus, according to the mechanism of metabolism, keratin 10 is expressed in the stratum spinosum and is useful in the metabolic process. The expression of keratin 10 indicates that normal metabolism is taking place.

[0125] In addition, there is a paper reporting that the cell proliferation and collagen expression in human skin fibroblasts are inhibited by blocking the transmission pathway of TGF-β by irradiating blue light (Ge Ge et al., "Induced skin aging by blue-light irradiation in human skin fibroblasts via TGF-β, JNK and EGFRpathways", Journal of Dermatological Science). From this point of view, it can be strongly speculated that the wavelength of 505nm is more advantageous than the wavelength of 470nm.

[0126] According to the above Figure 4A , Figure 4B and Figures 12 to 15 The test results shown show that irradiating the skin with light having a central wavelength in a wavelength range longer than 490nm and below 525nm is beneficial from the perspective of the melanin production inhibitory effect and the keratin 10 expression effect, compared with irradiating the skin with light having a central wavelength in other wavelength ranges.

[0127] The above embodiments are described in detail, but are not limited to specific embodiments, and various modifications and changes can be made within the scope of the claims. In addition, all or multiple components of the above embodiments can be combined.

[0128] Description of Reference Numerals

[0129] 1. Light irradiation device (skin treatment device)

[0130] 2. Handle

[0131] 3 Head

[0132] 3a Skin relative area

[0133] 30 light irradiation part

[0134] 36LED(Light Emitting Diode)

[0135] 100 control device (control unit).

Claims

1. A skin treatment device, characterized in that: have: A light source that generates a predetermined light having a central wavelength in a wavelength range longer than 490 nm and less than 525 nm, The skin treatment device can irradiate the predetermined light from the light source onto the skin.

2. The skin treatment device according to claim 1, characterized in that The prescribed light has a central wavelength of approximately 505 nm.

3. The skin treatment device according to claim 1 or 2, characterized in that: At 0.5mW / cm 2 Above and 62mW / cm 2 The prescribed light is irradiated at the following radiation intensity.

4. The skin treatment device according to claim 1 or 2, characterized in that: At 0.09J / cm 2 Above and 45J / cm 2 The prescribed light is irradiated with an irradiation energy in the following range.

5. The skin treatment device according to claim 1 or 2, characterized in that: The light source includes an LED.

6. The skin treatment device according to claim 1, characterized in that: The predetermined light can be irradiated in a manner that suppresses melanin production in the skin area of ​​the irradiated person, compared to light having a central wavelength outside the wavelength range.

7. The skin treatment device according to claim 1 or 2, characterized in that: The predetermined light can be irradiated to the irradiated human skin portion so as to have a melanin production inhibitory effect of 5% or more compared to a case where the predetermined light is not irradiated.

8. The skin treatment device according to claim 1 or 2, characterized in that: The skin treatment device further includes: a control unit that controls the irradiation of light in one or more action modes. The one or more action modes include a predetermined action mode corresponding to the melanin production inhibitory effect, or a predetermined action mode corresponding to an effect related to the melanin production inhibitory effect. The control unit outputs the predetermined light in the predetermined operation mode.

9. The skin treatment device according to claim 1 or 2, characterized in that: Continuous light irradiation of 1 minute or more can be performed in which the ratio of the irradiation time of the predetermined light is 1 / 2 or more.

10. A skin treatment method, characterized in that: The skin treatment method includes irradiating human skin with predetermined light having a central wavelength in a wavelength range longer than 490 nm and not more than 525 nm.

11. The skin treatment method according to claim 10, characterized in that: The prescribed light has a central wavelength of approximately 505 nm.

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