Antioxidant composition, firming and rejuvenating cream, preparation and application

Abstract

The present invention relates to the field of daily chemical technology, and more particularly to a firming and rejuvenating cream, its preparation, and its use. The composition comprises a solvent, a moisturizer, an emulsifier, a preservative, a thickener, an antioxidant, a skin conditioner, and a vegetable oil. The cream provided by the present invention instantly hydrates the skin and improves fine lines caused by dryness. It contains signal peptides and plant extracts, which can help restore skin structural support and enhance the firmness of facial contours. The vegetable oil can repair a damaged skin barrier, lock in moisture, and improve dryness and roughness.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemicals, and in particular to a firming and rejuvenating cream, and its preparation and application. Background Art

[0002] Wrinkles develop because the skin's structure and function change with aging or due to external factors. Collagen and elastin are key substances that maintain skin's firmness and elasticity. With aging, their production decreases, leading to sagging skin and the appearance of wrinkles. The epidermis gradually thins, making the skin more susceptible to external damage and wrinkling. Loss of subcutaneous fat in the face reduces the skin's support, making wrinkles more noticeable.

[0003] In addition, long-term exposure to sunlight can damage collagen and elastic fibers in the skin, leading to photoaging, which is one of the main external factors in the formation of wrinkles. The excessive production of free radicals can damage skin cells and accelerate the aging process.

[0004] The core goal of wrinkle skin care products is to improve existing wrinkles and delay the formation of new wrinkles, which is usually achieved by increasing skin moisturization, promoting collagen production, improving skin elasticity and fighting free radical damage.

[0005] Vitamin A derivatives (retinol, retinoic acid) can promote collagen synthesis, accelerate cell renewal, and improve fine lines and wrinkles. Peptides can stimulate collagen production and enhance skin elasticity. Collagen and elastin provide the structural support needed by the skin, but their absorption is limited when applied topically, making them more suitable as auxiliary ingredients.

[0006] The stability of cream-based skincare products is a crucial factor influencing their quality and efficacy. This stability encompasses physical, chemical, and microbiological stability, ensuring that the product remains effective and safe during use without significant changes in texture, color, or odor. The stability of creams is susceptible to environmental factors and changes in ingredients.

[0007] To address this, the present invention provides a firming and rejuvenating cream, its preparation and application. Summary of the Invention

[0008] In order to solve the above technical problems, the present invention provides a firming and rejuvenating cream, the raw materials for preparing the firming and rejuvenating cream include: a solvent, a moisturizer, an emulsifier, a preservative, a thickener, and an antioxidant composition.

[0009] As an embodiment of the present invention, the antioxidant composition comprises an antioxidant, a skin conditioner and plant oil.

[0010] As an embodiment of the present invention, the vegetable oil comprises sunflower (HELIANTHUS ANNUUS) seed oil, white meadowfoam (LIMNANTHES ALBA) seed oil, jojoba (SIMMONDSIA CHINENSIS) seed oil, wild soybean (GLYCINESOJA) oil, wrinkled carrageenan (CHONDRUS CRISPUS) powder, vetiver (VETIVERIA ZIZANIOIDES) root oil, and redwood (BIXA ORELLANA) seed oil.

[0011] As an embodiment of the present invention, the vegetable oil is prepared by fermentation.

[0012] As an embodiment of the present invention, the skin conditioner comprises tetradecylaminobutyrylvalinamidobutyric acid urea trifluoroacetate.

[0013] As an embodiment of the present invention, the raw materials for preparing the firming and rejuvenating cream include, in parts by mass:

[0014] Water 40-60 parts, Glycerin 4-8 parts, Isononyl Isononanoate 4-9 parts, Caprylic / Capric Triglyceride 4-9 parts, Triethylhexanoin 3-7 parts, Butylene Glycol 3-6 parts, Dimethicone 2-7 parts, Cetearyl Alcohol 3-6 parts, Tribehenin PEG-20 Esters 1.5-3.5 parts, Hydrogenated Coco-Glycerides 1-2.8 parts, Macrogol-8 1.5-3.5 parts, phytosterol / octyldodecanol lauroyl glutamate 0.8-2.5 parts, cetyl palmitate 0.6-1.8 parts, trehalose 0.5-2 parts, beeswax 0.8-3 parts, methyl methacrylate crosspolymer 0.5-2 parts, glyceryl stearate 0.45-0.9 parts, PEG-100 stearate 0.45-1.2 parts, cocoyl glucoside 0.2-1.2 parts, tocopheryl acetate Phenol acetate 0.3-1.2 parts, xylitol glucoside 0.25-0.55 parts, phenoxyethanol 0.3-0.6 parts, parahydroxyacetophenone 0.2-0.6 parts, polydimethylsiloxane / vinyl polydimethylsiloxane crosspolymer 0.3-0.8 parts, dehydrated xylitol 0.2-0.5 parts, triethanolamine 0.2-0.4 parts, carbomer 0.2-0.4 parts, sunflower (HELIANTHUS ANNUUS) seed oil 0.15-0.45 parts, allantoin 0.12-0.45 parts, bisabolol 0.15-0.3 parts, (daily use) flavor 0.15-0.3 parts, xanthan gum 0.12-0.2 parts, white meadowfoam (LIMNANTHES ALBA) seed oil 0.12-0.2 parts, jojoba (SIMMONDSIA CHINENSIS) seed oil 0.1-2 parts, xylitol 0.1-1 parts, dipotassium glycyrrhizate 0.05-0.15 parts, ammonium acryloyldimethyltaurate / VP copolymer 0.05-0.2 parts, disodium EDTA 0.05-1 parts, sodium PCA 0.02-0.4 parts, ethylhexylglycerin 0.02-0.1 parts, erythritol 0.02-1 parts, wild soybean (GLYCINE 0.01-0.1 part of SOJA) oil, 0.01-0.2 part of CHONDRUS CRISPUS powder, 0.01-0.05 part of carnosine, 0.005-1 part of sodium hyaluronate, 0.005-0.1 part of hydrolyzed collagen, 0.005-0.03 part of vetiver (VETIVERIAZIZANIOIDES) root oil, 0.002-0.02 part of benzoic acid, 0.001-0.1 part of sorbic acid, 0.001-0.2 part of tetradecylaminobutyryl valinamidobutyric acid uron trifluoroacetate, 0.001-0.12 part of hexapeptide-9, 0.0005-0.001 part of redwood (BIXA ORELLANA) seed oil, 0.0001-0.001 part of tocopherol (vitamin E), and 0.0001-0.001 part of magnesium chloride.

[0015] As an embodiment of the present invention, the raw materials for its preparation include, in parts by mass:

[0016] Water 40.3277 parts, Glycerin 6 parts, Isononyl Isononanoate 4 parts, Caprylic / Capric Triglyceride 6 parts, Triethylhexanoin 4 parts, Butylene Glycol 4.5 parts, Dimethicone 2.8 parts, Cetearyl Alcohol 3.2 parts, Tribehenin PEG-20 Esters 3 parts, Hydrogenated Coco-Glycerides 2.5 parts, Macrogol-8 3 parts, phytosterol / octyldodecanol lauroyl glutamate 2.3 parts, cetyl palmitate 1.6 parts, trehalose 1.5 parts, beeswax 2 parts, methyl methacrylate crosspolymer 1.5 parts, glyceryl stearate 1.8 parts, PEG-100 stearate 1.6 parts, cocoyl glucoside 1 part, tocopheryl acetate 1 part, xylitol glucoside 0.5 parts, phenoxyethanol 0.4 parts, parahydroxyacetophenone 0.4 parts, polydimethylsiloxane / vinyl polydimethylsiloxane crosspolymer 0.5 parts, dehydrated xylitol 0.33 parts, triethanolamine 0.3 parts, carbomer 0.3 parts, sunflower (HELIANTHUSANNUUS) seed oil 0.28 parts, allantoin 0.13 parts, bisabolol 0.16 parts, (daily use) essence 0.17 parts, xanthan gum 0.16 parts, white meadowfoam (LIMNANTHES ALBA) seed oil 0.15 parts, jojoba (simmondsiachinensis) seed oil 0.6 parts, xylitol 0.5 parts, dipotassium glycyrrhizate 0.1 parts, ammonium acryloyldimethyltaurate / VP copolymer 0.15 parts, disodium EDTA 0.3 parts, sodium PCA 0.2 parts, ethylhexylglycerin 0.05 parts, erythritol 0.06 parts, soybean (glycine soja) oil 0.03 parts, chondrus crispus (chondrus crispus) powder 0.1 parts, carnosine 0.04 parts, sodium hyaluronate 0.3 parts, hydrolyzed collagen 0.02 parts, vetiver (vetivella zizanioides) root oil 0.02 parts, benzoic acid 0.015 parts, sorbic acid 0.08 parts, tetradecylaminobutyryl valinamidobutyric acid uron trifluoroacetate 0.015 parts, hexapeptide-9 0.01 parts, annatto (Bixaorella) seed oil 0.0009 parts, tocopherol (vitamin E) 0.0008 parts, magnesium chloride 0.0006 parts.

[0017] The present invention provides a method for preparing the firming and rejuvenating cream, the steps of which are as follows:

[0018] S1. Mix water, glycerin, butylene glycol, polyethylene glycol-8, trehalose, xylitol glucoside, parahydroxyacetophenone, phenoxyethanol, anhydroxylitol, carbomer, allantoin, xanthan gum, xylitol, dipotassium glycyrrhizate, ammonium acryloyldimethyltaurate / VP copolymer, sodium hyaluronate, disodium EDTA, and ethylhexylglycerin; mix the above ingredients evenly, heat to 75-80°C and set aside to prepare phase A;

[0019] S2. Isononyl isononanoate, caprylic / capric triglyceride, triethylhexanoin, dimethicone, cetearyl alcohol, tribehenin PEG-20 esters, hydrogenated coconut glycerides, phytosteryl / octyldodecanol lauroyl glutamate, cetyl palmitate, beeswax, methyl methacrylate crosspolymer, glyceryl stearate, PEG-100 stearate, cocoyl glucoside, tocopheryl acetate, sunflower (Helianthus annuus) seed oil, bisabolol, meadowfoam (Limnanthes alba) seed oil, redwood (Bixa orellana) seed oil, soybean (Glycine soja) oil, tocopherol (vitamin E), and jojoba (Simmondsia chinensis) seed oil; mix the above ingredients evenly, heat to 75-80° C. and set aside to prepare Phase B.

[0020] S3. Add phase B to phase A and emulsify and homogenize for 5 minutes. After emulsification and homogenization, stir and cool to obtain phase A+B.

[0021] S4, triethanolamine is phase C, after phase A+B cools down to 60-65℃, add and continue stirring and cooling to form phase A+B+C;

[0022] S5. Evenly mix the (daily use) flavor, erythritol, Chondrus Crispus powder, carnosine, hydrolyzed collagen, Vetiveria zizzanioides root oil, benzoic acid, sorbic acid, tetradecylaminobutyryl valinamidobutyric acid urea trifluoroacetate, hexapeptide-9, sodium PCA, and magnesium chloride to form phase D.

[0023] S6. After the temperature of phase A+B+C is lowered to below 45°C, add phase D and stir evenly to obtain phase A+B+C+D.

[0024] S7, the product is discharged and filled.

[0025] As an embodiment of the present invention, in step S3, both phase A and phase B need to be heated to 75-80° C. and then mixed.

[0026] As an embodiment of the present invention, the firming and rejuvenating cream is used in the field of daily chemicals.

[0027] By adopting the above technical solution, the present invention has the following beneficial effects:

[0028] The facial cream provided by the present invention can instantly hydrate the skin and improve fine lines caused by dryness.

[0029] Contains signal peptides and plant extracts to help restore skin structural support and enhance the firmness of facial contours.

[0030] Contains plant oils that can repair damaged skin barriers, lock in moisture, and improve dryness and roughness. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0032] Figure 1 This is a photo of the under-eye wrinkles of VISIA-CR before testing the product in Example 1;

[0033] Figure 2 This is a photo of the under-eye wrinkles of VISIA-CR after testing the product in Example 1;

[0034] Figure 3 Skin redness image before product testing in Example 1;

[0035] Figure 4 Skin redness after product testing in Example 1. DETAILED DESCRIPTION

[0036] The technical solution of the present invention will be described clearly and completely below. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0037] The present invention provides the firming and rejuvenating cream, the raw materials for its preparation include:

[0038] Water 40.3277 parts, Glycerin 6 parts, Isononyl Isononanoate 4 parts, Caprylic / Capric Triglyceride 6 parts, Triethylhexanoin 4 parts, Butylene Glycol 4.5 parts, Dimethicone 2.8 parts, Cetearyl Alcohol 3.2 parts, Tribehenin PEG-20 Esters 3 parts, Hydrogenated Coco-Glycerides 2.5 parts, Macrogol-8 3 parts, phytosterol / octyldodecanol lauroyl glutamate 2.3 parts, cetyl palmitate 1.6 parts, trehalose 1.5 parts, beeswax 2 parts, methyl methacrylate crosspolymer 1.5 parts, glyceryl stearate 1.8 parts, PEG-100 stearate 1.6 parts, cocoyl glucoside 1 part, tocopheryl acetate 1 part, xylitol glucoside 0.5 parts, phenoxyethanol 0.4 parts, parahydroxyacetophenone 0.4 parts, polydimethylsiloxane / vinyl polydimethylsiloxane crosspolymer 0.5 parts, dehydrated xylitol 0.33 parts, triethanolamine 0.3 parts, carbomer 0.3 parts, sunflower (HELIANTHUSANNUUS) seed oil 0.28 parts, allantoin 0.13 parts, bisabolol 0.16 parts, (daily use) essence 0.17 parts, xanthan gum 0.16 parts, white meadowfoam (LIMNANTHES ALBA) seed oil 0.15 parts, jojoba (simmondsiachinensis) seed oil 0.6 parts, xylitol 0.5 parts, dipotassium glycyrrhizate 0.1 parts, ammonium acryloyldimethyltaurate / VP copolymer 0.15 parts, disodium EDTA 0.3 parts, sodium PCA 0.2 parts, ethylhexylglycerin 0.05 parts, erythritol 0.06 parts, soybean (glycine soja) oil 0.03 parts, Chondrus crispus (chondrus crispus) powder 0.1 parts, carnosine 0.04 parts, sodium hyaluronate 0.3 parts, hydrolyzed collagen 0.02 parts, vetiver (vetiva zizanioides) root oil 0.02 parts, benzoic acid 0.015 parts, sorbic acid 0.08 parts, tetradecylaminobutyryl valinamidobutyric acid uron trifluoroacetate 0.015 parts, hexapeptide-9 0.01 parts, annatto (Bixaorella) seed oil 0.0009 parts, tocopherol (vitamin E) 0.0008 parts, magnesium chloride 0.0006 parts.

[0039] In the present invention, the preparation method of the vegetable oil (including sunflower (Helianthus annuus) seed oil, white meadowfoam (Limnanthes alba) seed oil, jojoba (Simmondsia chinensis) seed oil, wild soybean (Glycine soja) oil, vetiver (Vetiveria zizanioides) root oil, and redwood (Bixa orellana) seed oil) is as follows:

[0040] (1) Wash and dry sunflower seeds, meadowfoam seeds, jojoba seeds, wild soybeans, vetiver roots, and mahogany seeds, grind and mix them (particle size 0.5-1 mm);

[0041] (2) microwave the pulverized mixture obtained in step (1) for 10 minutes and then cool it down;

[0042] (3) The crushed mixture and culture medium were mixed in a mass ratio of 1:1, inoculated with the bacteria, and fermented at 30°C for 24 h; during the fermentation, the mixture was turned over every 8 h;

[0043] (4) Subsequently, 9 times the mass of culture medium (based on the mass of the crushed mixture) and 0.5 times the mass of lecithin (based on the mass of the crushed mixture) were added, and fermentation was continued for 72 h with stirring at 50 rpm;

[0044] (5) After fermentation is complete, the fermentation broth is centrifuged at low speed (3000 rpm, 15 minutes) to separate the oil-water mixture and the solid residue. The oil-water mixture is further centrifuged at high speed (10000 rpm, 20 minutes) to separate the crude oil phase; the crude oil phase is then degummed, deodorized, and decolorized; and the crude oil is pasteurized at low temperature to obtain the vegetable oil.

[0045] The components of the culture medium in step (3) are: sucrose 30.0 g, NaNO3 3.0 g, MgSO4·7H2O 0.5 g, KCl 0.5 g, FeSO4·4H2O 0.01 g, K2HPO4 1.0 g, and distilled water 1000.0 mL.

[0046] The strain is: Aspergillus niger 40273), Saccharomyces cerevisiae 32883), and the inoculation ratio was 2:1; the comprehensive inoculation amount was 9%.

[0047] In the present invention, the preparation method of the Chondrus Crispus powder is as follows:

[0048] Dried Chondrus crispus is ground into 80-100 mesh size, mixed with water at a ratio of 1:10 (Cornus crispus powder:water), and stirred to form a Chondrus crispus culture solution. Subsequently, 20 g / L glucose, 8 g / L yeast extract, and 12 g / L potassium dihydrogen phosphate are added, followed by the addition of activated bacteria at a 5% (v / v) inoculum. The fermentation temperature is 37°C, and the dissolved oxygen ventilation rate is 1.0 vvm. The fermentation is continued for 72 hours (25 g / L glutamic acid is added after 24 hours of fermentation). After fermentation, the solution is centrifuged at 8000 rpm for 20 minutes to separate the fermentation supernatant and bacterial precipitate. The supernatant is dried to obtain the resulting powder, which is Chondrus crispus powder.

[0049] The strain is Bacillus subtilis, and the activation method is as follows: the Bacillus subtilis is cultured in LB medium (tryptone 10g / L, yeast powder 5g / L, NaCl 10g / L, pH 7.0). The culture is shaken at 37°C and 150 rpm for 12 hours to obtain a highly active bacterial solution (concentration of 1*10 9 CFU / mL).

[0050] In the present invention, the polydimethylsiloxane is a mixture of 1.5 viscosity silicone oil and 350 viscosity silicone oil in a mass ratio of 1:9. 1.5 viscosity silicone oil: RH-201-1.5, Zhejiang Runhe Silicone New Materials Co., Ltd.; 350 viscosity silicone oil: XIAMETER TM PMX-200Silicone Fluid 350cSt, Dow (Zhangjiagang) Investment Co., Ltd.

[0051] Tetradecylaminobutyrylvalinamidobutyric acid urea trifluoroacetate is effective, but it is relatively sensitive to ultraviolet light, heat, and acid-base environments. High-humidity ingredients (such as glycerol, propylene glycol, and hyaluronic acid) may affect the stability of tetradecylaminobutyrylvalinamidobutyric acid urea trifluoroacetate. High-humidity ingredients are highly hygroscopic and increase the proportion of free water in the formula. Excessive moisture weakens the hydrophobic interactions between molecules, making the structure of tetradecylaminobutyrylvalinamidobutyric acid urea trifluoroacetate more susceptible to disruption. The present invention ferments Chondrus crispus powder and limits the molecular weight of polysiloxane, which can form weak hydrogen bonds with tetradecylaminobutyrylvalinamidobutyric acid urea trifluoroacetate, stabilizing the molecular structure. It also reduces the possibility of tetradecylaminobutyrylvalinamidobutyric acid urea trifluoroacetate being exposed to free water, inhibiting hydrolysis.

[0052] Chondrus crispus powder interacts with cationic components or sodium PCA to form gel or flocs; therefore, fermenting the Chondrus crispus powder can improve this phenomenon.

[0053] The present invention will be further explained below with reference to specific embodiments.

[0054] Example 1

[0055] This embodiment provides the firming and rejuvenating cream, the raw materials for its preparation include:

[0056] Water 40.3277 parts, Glycerin 6 parts, Isononyl Isononanoate 4 parts, Caprylic / Capric Triglyceride 6 parts, Triethylhexanoin 4 parts, Butylene Glycol 4.5 parts, Dimethicone 2.8 parts, Cetearyl Alcohol 3.2 parts, Tribehenin PEG-20 Esters 3 parts, Hydrogenated Coco-Glycerides 2.5 parts, Macrogol-8 3 parts, phytosterol / octyldodecanol lauroyl glutamate 2.3 parts, cetyl palmitate 1.6 parts, trehalose 1.5 parts, beeswax 2 parts, methyl methacrylate crosspolymer 1.5 parts, glyceryl stearate 1.8 parts, PEG-100 stearate 1.6 parts, cocoyl glucoside 1 part, tocopheryl acetate 1 part, xylitol glucoside 0.5 parts, phenoxyethanol 0.4 parts, parahydroxyacetophenone 0.4 parts, polydimethylsiloxane / vinyl polydimethylsiloxane crosspolymer 0.5 parts, dehydrated xylitol 0.33 parts, triethanolamine 0.3 parts, carbomer 0.3 parts, sunflower (HELIANTHUSANNUUS) seed oil 0.28 parts, allantoin 0.13 parts, bisabolol 0.16 parts, (daily use) essence 0.17 parts, xanthan gum 0.16 parts, white meadowfoam (LIMNANTHES ALBA) seed oil 0.15 parts, jojoba (SIMMONDSIA CHINENSIS) seed oil 0.6 parts, xylitol 0.5 parts, dipotassium glycyrrhizate 0.1 parts, ammonium acryloyldimethyltaurate / VP copolymer 0.15 parts, disodium EDTA 0.3 parts, sodium PCA 0.2 parts, ethylhexylglycerin 0.05 parts, erythritol 0.06 parts, soybean (GLYCINE SOJA) oil 0.03 parts, Chondrus Crispus powder 0.1 parts, carnosine 0.04 parts, sodium hyaluronate 0.3 parts, hydrolyzed collagen 0.02 parts, vetiver (VETIVERIA ZIZANIOIDES) root oil 0.02 parts, benzoic acid 0.015 parts, sorbic acid 0.08 parts, tetradecylaminobutyryl valinamidobutyric acid uron trifluoroacetate 0.015 parts, hexapeptide-9 0.01 parts, annatto (Bixaorella) seed oil 0.0009 parts, tocopherol (vitamin E) 0.0008 parts, magnesium chloride 0.0006 parts.

[0057] In this embodiment, the preparation method of the vegetable oil (including sunflower (Helianthus annuus) seed oil, white meadowfoam (Limnanthes alba) seed oil, jojoba (Simmonsia chinensis) seed oil, wild soybean (Glycine soja) oil, vetiver (Vetiveria zizanioides) root oil, and redwood (Bixa orellana) seed oil) is as follows:

[0058] (1) Wash and dry sunflower seeds, meadowfoam seeds, jojoba seeds, wild soybeans, vetiver roots, and mahogany seeds, grind and mix them (particle size 0.5-1 mm);

[0059] (2) microwave the pulverized mixture obtained in step (1) for 10 minutes and then cool it down;

[0060] (3) Mix the crushed mixture and culture medium in a mass ratio of 1:1, inoculate the bacteria, and ferment at 30°C for 24 hours; turn the mixture over every 8 hours during fermentation;

[0061] (4) Subsequently, 9 times the mass of culture medium (based on the mass of the crushed mixture) and 0.5 times the mass of lecithin (based on the mass of the crushed mixture) were added, and fermentation was continued for 72 h with stirring at 50 rpm;

[0062] (5) After fermentation is complete, the fermentation broth is centrifuged at low speed (3000 rpm, 15 minutes) to separate the oil-water mixture and the solid residue. The oil-water mixture is further centrifuged at high speed (10000 rpm, 20 minutes) to separate the crude oil phase; the crude oil phase is then degummed, deodorized, and decolorized; and the crude oil is pasteurized at low temperature to obtain the vegetable oil.

[0063] The components of the culture medium in step (3) are: sucrose 30.0 g, NaNO3 3.0 g, MgSO4·7H2O 0.5 g, KCl 0.5 g, FeSO4·4H2O 0.01 g, K2HPO4 1.0 g, and distilled water 1000.0 mL.

[0064] The strain is: Aspergillus niger 40273), Saccharomyces cerevisiae 32883), and the inoculation ratio was 2:1; the comprehensive inoculation amount was 9%.

[0065] In this embodiment, the preparation method of the Chondrus Crispus powder is as follows:

[0066] Dried Chondrus crispus is ground into 80-100 mesh size, mixed with water at a ratio of 1:10 (Cornus crispus powder:water), and stirred to form a Chondrus crispus culture solution. Subsequently, 20 g / L glucose, 8 g / L yeast extract, and 12 g / L potassium dihydrogen phosphate are added, followed by the addition of activated bacteria at a 5% (v / v) inoculum. The fermentation temperature is 37°C, and the dissolved oxygen ventilation rate is 1.0 vvm. The fermentation is continued for 72 hours (25 g / L glutamic acid is added after 24 hours of fermentation). After fermentation, the solution is centrifuged at 8000 rpm for 20 minutes to separate the fermentation supernatant and bacterial precipitate. The supernatant is dried to obtain the resulting powder, which is Chondrus crispus powder.

[0067] The strain is Bacillus subtilis, and the activation method is as follows: the Bacillus subtilis is cultured in LB medium (tryptone 10g / L, yeast powder 5g / L, NaCl 10g / L, pH 7.0). The culture is shaken at 37°C and 150 rpm for 12 hours to obtain a highly active bacterial solution (concentration of 1*10 9 CFU / mL).

[0068] In this embodiment, the polydimethylsiloxane is a mixture of 1.5 viscosity silicone oil and 350 viscosity silicone oil in a mass ratio of 1:9. 1.5 viscosity silicone oil: RH-201-1.5, Zhejiang Runhe Silicone New Materials Co., Ltd.; 350 viscosity silicone oil: XIAMETER TM PMX-200Silicone Fluid 350cSt, Dow (Zhangjiagang) Investment Co., Ltd.

[0069] This embodiment provides a method for preparing the firming and rejuvenating cream, and the steps are as follows:

[0070] S1. Mix water, glycerin, butylene glycol, polyethylene glycol-8, trehalose, xylitol glucoside, parahydroxyacetophenone, phenoxyethanol, anhydroxylitol, carbomer, allantoin, xanthan gum, xylitol, dipotassium glycyrrhizate, ammonium acryloyldimethyltaurate / VP copolymer, sodium hyaluronate, disodium EDTA, and ethylhexylglycerin; mix the above ingredients evenly, heat to 75-80°C and set aside to prepare phase A;

[0071] S2. Isononyl isononanoate, caprylic / capric triglyceride, triethylhexanoin, dimethicone, cetearyl alcohol, tribehenin PEG-20 esters, hydrogenated coconut glycerides, phytosteryl / octyldodecanol lauroyl glutamate, cetyl palmitate, beeswax, methyl methacrylate crosspolymer, glyceryl stearate, PEG-100 stearate, cocoyl glucoside, tocopheryl acetate, sunflower (Helianthus annuus) seed oil, bisabolol, meadowfoam (Limnanthes alba) seed oil, redwood (Bixa orellana) seed oil, soybean (Glycine soja) oil, tocopherol (vitamin E), and jojoba (Simmondsia chinensis) seed oil; mix the above ingredients evenly, heat to 75-80° C. and set aside to prepare Phase B.

[0072] S3. Add phase B to phase A and emulsify and homogenize for 5 minutes. After emulsification and homogenization, stir and cool to obtain phase A+B.

[0073] S4, triethanolamine is phase C, after phase A+B cools down to 60-65℃, add and continue stirring and cooling to form phase A+B+C;

[0074] S5. Evenly mix the daily flavor, erythritol, Chondrus Crispus powder, carnosine, hydrolyzed collagen, Vetiver (Vetivetia Zizanioides) root oil, benzoic acid, sorbic acid, tetradecylaminobutyryl valinamidobutyric acid urea trifluoroacetate, hexapeptide-9, sodium PCA, and magnesium chloride to form Phase D.

[0075] S6. After the temperature of phase A+B+C is lowered to below 45°C, add phase D and stir evenly to obtain phase A+B+C+D.

[0076] S7, the product is discharged and filled.

[0077] Example 2

[0078] The difference between this embodiment and embodiment 1 is that the preparation method of the vegetable oil (including sunflower (Helianthus annuus) seed oil, white meadowfoam (Limanthus alba) seed oil, jojoba (Simmonsia chinensis) seed oil, wild soybean (Glycine soja) oil, vetiver (Vetiveria zizanioides) root oil, and redwood (Bixa orellana) seed oil) is as follows:

[0079] (1) Wash and dry sunflower seeds, meadowfoam seeds, jojoba seeds, wild soybeans, vetiver roots, and mahogany seeds, then grind them into powder (particle size 0.5-1 mm).

[0080] (2) microwave the pulverized products obtained in step (1) for 10 min and then cool them down;

[0081] (3) The microwave-treated pulverized material was mixed with the culture medium at a mass ratio of 1:1, inoculated with the bacteria, and fermented at 30°C for 24 hours; during the fermentation, the mixture was turned over every 8 hours;

[0082] (4) Then, 9 times the weight of culture medium (based on the weight of each crushed material) and 0.5 times the weight of lecithin (based on the weight of each crushed material) were added, and fermentation was continued for 72 h with stirring at 50 rpm;

[0083] (5) After fermentation is complete, the fermentation broth is centrifuged at low speed (3000 rpm, 15 minutes) to separate the oil-water mixture and the solid residue. The oil-water mixture is further centrifuged at high speed (10000 rpm, 20 minutes) to separate the crude oil phase; the crude oil phase is then degummed, deodorized, and decolorized; and the crude oil is pasteurized at low temperature to obtain the respective vegetable oils.

[0084] The components of the culture medium in step (3) are: sucrose 30.0 g, NaNO3 3.0 g, MgSO4·7H2O 0.5 g, KCl 0.5 g, FeSO4·4H2O 0.01 g, K2HPO4 1.0 g, and distilled water 1000.0 mL.

[0085] The strain is: Aspergillus niger 40273), Saccharomyces cerevisiae 32883), and the inoculation ratio was 2:1; the comprehensive inoculation amount was 9%.

[0086] The vegetable oils described in this example were fermented separately to obtain independent sunflower (HELIANTHUS ANNUUS) seed oil, white meadowfoam (LIMNANTHES ALBA) seed oil, jojoba (SIMMONDSIA CHINENSIS) seed oil, wild soybean (GLYCINESOJA) oil, vetiver (VETIVERIA ZIZANIOIDES) root oil, and redwood (BIXA ORELLANA) seed oil.

[0087] Example 3

[0088] The difference between Example 3 and Example 1 is that the preparation method of the Chondrus crispus powder is as follows: dried Chondrus crispus is crushed into 80-100 meshes to obtain the powder.

[0089] Example 4

[0090] The difference between Example 4 and Example 1 is that in this example, the polydimethylsiloxane is 350 viscosity silicone oil; 350 viscosity, silicone oil: XIAMETER TM PMX-200Silicone Fluid 350cSt, Dow (Zhangjiagang) Investment Co., Ltd.

[0091] Example 5

[0092] The difference between Example 5 and Example 1 is that this example provides a method for preparing the firming and rejuvenating cream, and the steps are as follows:

[0093] S1. Combine water, glycerin, butylene glycol, polyethylene glycol-8, trehalose, xylitol glucoside, parahydroxyacetophenone, phenoxyethanol, anhydroxylitol, carbomer, allantoin, xanthan gum, xylitol, dipotassium glycyrrhizate, ammonium acryloyldimethyltaurate / VP copolymer, sodium hyaluronate, disodium EDTA, ethylhexylglycerin, and Chondrus crispus powder; mix the above ingredients evenly and heat to 75-80° C. for later use. This is phase A.

[0094] S2. Isononyl isononanoate, caprylic / capric triglyceride, triethylhexanoin, dimethicone, cetearyl alcohol, tribehenin PEG-20 esters, hydrogenated coconut glycerides, phytosteryl / octyldodecanol lauroyl glutamate, cetyl palmitate, beeswax, methyl methacrylate crosspolymer, glyceryl stearate, PEG-100 stearate, cocoyl glucoside, tocopheryl acetate, sunflower (Helianthus annuus) seed oil, bisabolol, meadowfoam (Limnanthes alba) seed oil, redwood (Bixa orellana) seed oil, soybean (Glycine soja) oil, tocopherol (vitamin E), jojoba (Simmondsia chinensis) seed oil, and vetiver (Vetiveria zizanioides) root oil; mix the above ingredients evenly, heat to 75-80° C. and set aside to prepare Phase B.

[0095] S3. Add phase B to phase A and emulsify and homogenize for 5 minutes. After emulsification and homogenization, stir and cool to obtain phase A+B.

[0096] S4, triethanolamine is phase C, after phase A+B cools down to 60-65℃, add and continue stirring and cooling to form phase A+B+C;

[0097] S5. Evenly mix the (daily use) flavor, erythritol, carnosine, hydrolyzed collagen, benzoic acid, sorbic acid, tetradecylaminobutyryl valinamidobutyric acid urea trifluoroacetate, hexapeptide-9, sodium PCA, and magnesium chloride to form phase D.

[0098] S6. After the temperature of phase A+B+C is lowered to below 45°C, add phase D and stir evenly to obtain phase A+B+C+D.

[0099] S7, the product is discharged and filled.

[0100] Example 6

[0101] The difference between Example 6 and Example 1 is that this example provides the firming and rejuvenating cream, and the raw materials for its preparation include:

[0102] Water 40.3277 parts, Glycerin 6 parts, Isononyl Isononanoate 4 parts, Caprylic / Capric Triglyceride 6 parts, Triethylhexanoin 4 parts, Butylene Glycol 4.5 parts, Cetearyl Alcohol 1.8 parts, Tribehenin PEG-20 Esters 2.9 parts, Hydrogenated Coco-Glycerides 2.5 parts, Macrogol-8 3 parts, phytosterol / octyldodecanol lauroyl glutamate 2.3 parts, cetyl palmitate 1.6 parts, trehalose 1.5 parts, beeswax 2 parts, methyl methacrylate crosspolymer 1.5 parts, glyceryl stearate 1.8 parts, PEG-100 stearate 4.9 parts, cocoyl glucoside 1 part, tocopheryl acetate 1 part, xylitol glucoside 0.5 parts, phenoxyethanol 0.4 parts, parahydroxyacetophenone 0.4 parts, polydimethylsiloxane / vinyl polydimethylsiloxane crosspolymer 0.5 parts, dehydrated xylitol 0.33 parts, triethanolamine 0.3 parts, carbomer 0.3 parts, sunflower (HELIANTHUS ANNUUS) seed oil 0.28 parts, allantoin 0.13 parts, bisabolol 0.16 parts, (daily use) essence 0.17 parts, xanthan gum 0.16 parts, white meadowfoam (LIMNANTHES ALBA) seed oil 0.65 parts, simmondsia chinensis (jojoba) seed oil 0.6 parts, xylitol 0.5 parts, dipotassium glycyrrhizate 0.1 parts, ammonium acryloyldimethyltaurate / VP copolymer 0.15 parts, disodium EDTA 0.3 parts, sodium PCA 0.2 parts, ethylhexylglycerin 0.05 parts, erythritol 0.06 parts, soybean (glycine soja) oil 0.53 parts, chondrus crispus (chondrus crispus) powder 0.1 parts, carnosine 0.04 parts, sodium hyaluronate 0.3 parts, hydrolyzed collagen 0.02 parts, vetiver (vetiva zizanioides) root oil 0.02 parts, benzoic acid 0.015 parts, sorbic acid 0.08 parts, tetradecylaminobutyryl valinamidobutyric acid uron trifluoroacetate 0.015 parts, hexapeptide-9 0.01 parts, 0.0009 parts of redwood (BIXA ORELLANA) seed oil, 0.0008 parts of tocopherol (vitamin E), and 0.0006 parts of magnesium chloride.

[0103] Example 7

[0104] The difference between Example 7 and Example 1 is that this example provides the firming and rejuvenating cream, and the raw materials for its preparation include:

[0105] Water 40.3277 parts, Glycerin 6 parts, Isononyl Isononanoate 4 parts, Caprylic / Capric Triglyceride 6 parts, Triethylhexanoin 4 parts, Butylene Glycol 4.5 parts, Dimethicone 6.6 parts, Cetearyl Alcohol 2.5 parts, Tribehenin PEG-20 Esters 2 parts, Hydrogenated Coco-Glycerides 2.5 parts, Macrogol-8 3 parts, phytosterol / octyldodecanol lauroyl glutamate 2.3 parts, cetyl palmitate 1.6 parts, trehalose 1.5 parts, beeswax 2 parts, methyl methacrylate crosspolymer 1.5 parts, glyceryl stearate 1.8 parts, cocoyl glucoside 1 part, tocopheryl acetate 1 part, xylitol glucoside 0.5 parts, phenoxyethanol 0.4 parts, p-hydroxyacetophenone 0.4 parts, dehydrated xylitol 0.33 parts, triethanolamine 0.3 parts, carbomer 0.3 parts, sunflower (HELIANTHUS ANNUUS) seed oil 0.28 parts, allantoin 0.13 parts, bisabolol 0.16 parts, (daily use) essence 0.17 parts, xanthan gum 0.16 parts, white meadow flower (LIMNANTHES ALBA) seed oil 0.15 parts, jojoba (SIMMONDSIA 0.6 parts of Glycyrrhizic Acid (CHINENSIS) Seed Oil, 0.5 parts of Xylitol, 0.1 parts of Dipotassium Glycyrrhizate, 0.15 parts of Ammonium Acryloyldimethyltaurate / VP Copolymer, 0.3 parts of Disodium EDTA, 0.2 parts of Sodium PCA, 0.05 parts of Ethylhexylglycerin, 0.06 parts of Erythritol, 0.03 parts of Glycine Soja (Soybean) Oil, 0.1 parts of Chondrus Crispus Powder, 0.04 parts of Carnosine, 0.3 parts of Sodium Hyaluronate, 0.02 parts of Hydrolyzed Collagen, 0.02 parts of Vetiver (VETIVERIA ZIZANIOIDES) Root Oil, 0.015 parts of Benzoic Acid, 0.08 parts of Sorbic Acid, 0.015 parts of Tetradecylaminobutyryl Valylamidobutyric Acid Urea Trifluoroacetate, Hexapeptide-9 0.01 parts, annatto (Bixaorella) seed oil 0.0009 parts, tocopherol (vitamin E) 0.0008 parts, magnesium chloride 0.0006 parts.

[0106] Performance Testing

[0107] Test 1: The creams prepared in Examples 1 to 7 were placed at 5°C, 25°C, and 40°C for 90 days, and the product properties and color were checked. The test results are shown in Table 1.

[0108] Table 1 Test results of performance test 1

[0109]

[0110]

[0111] Test 2: Hyaluronidase inhibition assay

[0112] The test is based on the HMC-W1-029 hyaluronidase inhibition rate.

[0113] Example 1-7: Dilute with pure water to a sample concentration of 0.2%;

[0114] Positive control (dipotassium glycyrrhizate, purity ≥98%): Dilute with water to a positive control concentration of 3%;

[0115] Negative control: pure water.

[0116] Test operation steps:

[0117] Design the embodiment group, positive control group and negative control group, and set up 3 parallels for each group. Add different reagent solutions respectively, shake well, and let stand at room temperature for 30 minutes for color development. Use ultraviolet spectrophotometer to measure the absorbance value at a wavelength of 528nm.

[0118] Calculation formula

[0119] Hyaluronidase inhibition rate (%) = (1-(CD) / (AB)) × 100

[0120] Wherein, A is the absorbance of the reaction solution without sample; B is the absorbance of the reaction solution without sample and enzyme; C is the absorbance of the reaction solution with sample and enzyme; D is the absorbance of the reaction solution with sample and no enzyme.

[0121] Statistical analysis software was used in SPSS. Comparisons of hyaluronidase inhibition rates among the test samples, positive controls, and negative controls were performed using independent t-tests. All statistical analyses were two-tailed, with a significance level of α = 0.05. A P > 0.05 indicated no significant difference between the two groups, and a P < 0.05 indicated a significant difference between the two groups.

[0122] Table 2 Test results

[0123] name Experimental results P-value Example 1 70.315±0.427 ＜0.05 Example 2 66.742±1.252 ＜0.05 Example 3 58.431±1.043 ＜0.05 Example 4 59.326±0.932 ＜0.05

[0124]

[0125] The hyaluronidase inhibition rate was rounded to three decimal places, and P < 0.05 indicated that there were significant differences between the sample and the positive control compared with the negative control.

[0126] Test 3: Elastase inhibition rate

[0127] Examples 1 to 7: Dilute with pure water to a sample concentration of 5%;

[0128] Positive control (epigallocatechin gallate): Dilute with water to a positive control concentration of 0.1%;

[0129] Negative control: pure water.

[0130] Test operation steps:

[0131] Design sample group, sample background group, solvent group and solvent background group. Set up 3 parallel groups for each group. Add different reagent solutions into the 96-well plate, shake gently, incubate at 25℃ for 15 minutes, place it in the enzyme reader, and measure the absorbance at 410nm.

[0132] Calculation formula: Elastase inhibition rate (%) = (1-(CD) / (AB)) × 100

[0133] Wherein, A is the absorbance of the reaction solution without sample; B is the absorbance of the reaction solution without sample and enzyme; C is the absorbance of the reaction solution with sample and enzyme; D is the absorbance of the reaction solution with sample and no enzyme.

[0134] Statistical analysis software was used in SPSS. Comparisons of elastase inhibition rates between the test samples, positive controls, and negative controls were performed using independent sample t-tests. All statistical analyses were two-tailed, with a significance level of α = 0.05. A P > 0.05 indicated no significant difference between the two groups, and a P < 0.05 indicated a significant difference between the two groups.

[0135] Table 3 Test results

[0136]

[0137]

[0138] The elastase inhibition rate was rounded to three decimal places, and P < 0.05 indicated that there were significant differences between the sample and the positive control compared with the negative control.

[0139] Test 4: Human anti-wrinkle and firming efficacy evaluation test

[0140] Take an appropriate amount of this product and apply it evenly to the skin around the eyes. Gently massage along the skin texture around the eyes until absorbed. Frequency of use: once a day in the morning and once in the evening.

[0141] Number of subjects: 30 people participated, 30 people were actually included, and the effectiveness rate was 100%.

[0142] Gender of subjects: 4 males and 26 females.

[0143] Age of subjects: 32 to 65 years old, average 43.15 years old.

[0144] Test area: corner of eye.

[0145] Test environment: temperature 21±1℃, relative humidity 50±10%RH.

[0146] Testing period: 28 days.

[0147] Before using the sample

[0148] One week before the test, the subjects stopped using facial cosmetics or topical medications. The night before the test, the subjects washed their faces at home and did not apply any skincare products. On the morning of the test, the subjects washed their faces with clean water at home and did not apply any skincare products. Qualified subjects completed the informed consent form.

[0149] The subjects cleaned their faces with water and rested in a constant temperature and humidity chamber (temperature 21 ± 1°C, relative humidity 50 ± 10% RH) for 30 minutes;

[0150] Conduct PRIMOS-CR photography test;

[0151] Use VISIA-7 to take photos;

[0152] Use the Cutometer to measure the skin elasticity R2 value and skin firmness F4 value; distribute test samples and instructions for use.

[0153] After using the sample for 28 days

[0154] After cleaning their faces with water, the subjects rested for 30 minutes in a constant temperature and humidity chamber (temperature 21 ± 1°C, relative humidity 50 ± 10% RH);

[0155] Conducted PRIMOS-CR test; took photos using VISIA-7;

[0156] The skin elasticity tester Cutometer was used to measure the skin elasticity R2 value and skin firmness F4 value;

[0157] Average depth test of crow's feet: average improvement rate = (average value before use - average value after use) / (average value before use) * 100%.

[0158] Crow's feet wrinkle number test: average improvement rate = (average value before use - average value after use) / (average value before use) * 100%.

[0159] Crow's feet volume test: average improvement rate = (average value before use - average value after use) / (average value before use) * 100%.

[0160] Crow's feet length test: average improvement rate = (average value before use - average value after use) / (average value before use) * 100%.

[0161] Skin elasticity R2 value test: average improvement rate = (average value after use - average value before use) / (average value before use) * 100%.

[0162] Skin firmness F4 value test: average improvement rate = (average value before use - average value after use) / (average value before use) * 100%.

[0163] Table 4 Test results

[0164]

[0165] In the accompanying drawings, Figure 1 This is a photo of the under-eye wrinkles of VISIA-CR before testing the product in Example 1; Figure 2 This is a photo of the under-eye wrinkles of VISIA-CR after testing the product in Example 1; Figure 3 Skin redness image before product testing in Example 1; Figure 4 Skin redness after product testing in Example 1.

[0166] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. Firming and Rejuvenating Cream, characterized by: The raw materials for its preparation include: Water 40.3277 parts, Glycerin 6 parts, Isononyl Isononanoate 4 parts, Caprylic / Capric Triglyceride 6 parts, Triethylhexanoin 4 parts, Butylene Glycol 4.5 parts, Dimethicone 2.8 parts, Cetearyl Alcohol 3.2 parts, Tribehenin PEG-20 Esters 3 parts, Hydrogenated Coco-Glycerides 2.5 parts, Macrogol-8 3 parts, Phytosteryl / Octyldodecyl Lauroyl Glutamate 2.3 parts, Cetyl Palmitate 1.6 parts, Trehalose 1.5 parts, Beeswax 2 parts, Methyl Methacrylate Crosspolymer 1.5 parts, Glyceryl Stearate 1.8 parts, PEG-100 1.6 parts of stearate, 1 part of cocoyl glucoside, 1 part of tocopheryl acetate, 0.5 parts of xylitol glucoside, 0.4 parts of phenoxyethanol, 0.4 parts of hydroxyacetophenone, 0.5 parts of polydimethylsiloxane / vinyl polydimethylsiloxane crosspolymer, 0.33 parts of dehydrated xylitol, 0.3 parts of triethanolamine, 0.3 parts of carbomer, 0.28 parts of sunflower (HELIANTHUS ANNUUS) seed oil, 0.13 parts of allantoin, 0.16 parts of bisabolol, 0.17 parts of (daily use) fragrance, 0.16 parts of xanthan gum, 0.15 parts of white meadowfoam (LIMNANTHES ALBA) seed oil, 0.6 parts of jojoba (SIMMONDSIA CHINENSIS) seed oil, 0.5 parts of xylitol, 0.1 parts of dipotassium glycyrrhizate, 0.15 parts of ammonium acryloyldimethyltaurate / VP copolymer, EDTA Disodium EDTA 0.3 parts, Sodium PCA 0.2 parts, Ethylhexylglycerin 0.05 parts, Erythritol 0.06 parts, Glycine Soja (Soybean) Oil 0.03 parts, Chondrus Crispus (Corn) Powder 0.1 parts, Carnosine 0.04 parts, Sodium Hyaluronate 0.3 parts, Hydrolyzed Collagen 0.02 parts, Vetiver (Vetiver) Root Oil 0.02 parts, Benzoic Acid 0.015 parts, Sorbic Acid 0.08 parts, Tetradecylaminobutyryl Valylamidobutyric Acid Urea Trifluoroacetate 0.015 parts, Hexapeptide-9 0.01 parts, Bixa Orellana (Redwood) Seed Oil 0.0009 parts, Tocopherol (Vitamin E), Magnesium Chloride 0.0006 parts; The vegetable oils, including sunflower (Helianthus annuus) seed oil, white meadowfoam (Limanthus alba) seed oil, jojoba (Simmonsia chinensis) seed oil, soybean (Glycine soja) oil, vetiveria zizzanioides root oil, and redwood (Bixa orellana) seed oil, are prepared as follows: (1) Wash and dry sunflower seeds, meadowfoam seeds, jojoba seeds, wild soybeans, vetiver roots, and redwood seeds, grind and mix them; (2) microwave the pulverized mixture obtained in step (1) for 10 min and then cool it down; (3) Mix the crushed mixture and culture medium in a mass ratio of 1:1, inoculate the bacteria, and ferment at 30°C for 24 hours; turn the mixture over every 8 hours during fermentation; (4) Then, 9 times the weight of culture medium and 0.5 times the weight of lecithin were added, and fermentation was continued for 72 h with stirring at 50 rpm; (5) After the fermentation is completed, the fermentation liquid is centrifuged at low speed to separate the oil-water mixed phase and the solid residue; the oil-water mixed phase is further centrifuged at high speed to separate the crude oil phase; then degumming, deodorization, and decolorization are performed; and low-temperature pasteurization is performed to obtain the vegetable oil; The components of the culture medium in step (3) are: sucrose 30.0 g, NaNO3 3.0 g, MgSO4·7H2O 0.5 g, KCl 0.5 g, FeSO4·4H2O 0.01 g, K2HPO4 1.0 g, and distilled water 1000.0 mL; The strains are: Aspergillus niger CICC®40273 and Saccharomyces cerevisiae CICC®32883, and the inoculation ratio is 2:1; the total inoculation amount is 9%; The preparation method of the Chondrus Crispus powder is as follows: The dried Chondrus crispus was crushed into 80-100 mesh, mixed with water at a ratio of 1:10, and stirred to form a Chondrus crispus culture solution. Subsequently, 20 g / L glucose, 8 g / L yeast powder, and 12 g / L potassium dihydrogen phosphate were added, and the activated bacteria were added at a 5% v / v inoculum. The fermentation temperature was 37°C and the dissolved oxygen ventilation volume was 1.0 vvm. The fermentation time was 72 hours. After 24 hours of fermentation, 25 g / L glutamic acid was added. After fermentation, the supernatant and bacterial precipitate were separated by centrifugation at 8000 rpm for 20 minutes. The supernatant was dried to obtain the Chondrus crispus powder. The bacterial strain is Bacillus subtilis, and the activation method is as follows: Bacillus subtilis is cultured in LB medium at 37°C and 150 rpm for 12 hours to obtain a highly active bacterial solution.

2. The method for preparing the firming and rejuvenating cream according to claim 1, wherein: The steps are as follows: S1. Mix water, glycerin, butylene glycol, polyethylene glycol-8, trehalose, xylitol glucoside, parahydroxyacetophenone, phenoxyethanol, anhydroxylitol, carbomer, allantoin, xanthan gum, xylitol, dipotassium glycyrrhizate, ammonium acryloyldimethyltaurate / VP copolymer, sodium hyaluronate, disodium EDTA, and ethylhexylglycerin; mix the above ingredients evenly and heat to 75-80°C for later use; this is phase A. S2. Combine isononyl isononanoate, caprylic / capric triglyceride, triethylhexanoin, dimethicone, cetearyl alcohol, tribehenin PEG-20 esters, hydrogenated coconut glycerides, phytosteryl / octyldodecanol lauroyl glutamate, cetyl palmitate, beeswax, methyl methacrylate crosspolymer, glyceryl stearate, PEG-100 stearate, cocoyl glucoside, tocopheryl acetate, sunflower (Helianthus annuus) seed oil, bisabolol, meadowfoam (Limnanthes alba) seed oil, redwood (Bixa orellana) seed oil, soybean (Glycine soja) oil, tocopherol (vitamin E), and jojoba (Simmondsia chinensis) seed oil; mix the above ingredients evenly and heat to 75-80°C for later use. This is phase B. S3. Add phase B to phase A and emulsify and homogenize for 5 minutes. After emulsification and homogenization, stir and cool to obtain phase A+B. S4, triethanolamine is phase C, after phase A+B cools down to 60-65℃, add and continue stirring and cooling to form phase A+B+C; S5. Evenly mix the (daily use) flavor, erythritol, Chondrus Crispus powder, carnosine, hydrolyzed collagen, Vetiver (Vetivetia Zizanioides) root oil, benzoic acid, sorbic acid, tetradecylaminobutyryl valinamidobutyric acid urea trifluoroacetate, hexapeptide-9, sodium PCA, and magnesium chloride to form Phase D. S6. After the temperature of phase A+B+C is lowered to below 45°C, add phase D and stir evenly to obtain phase A+B+C+D. S7, the product is discharged and filled.

3. The firming and rejuvenating cream according to claim 1, characterized in that Used in daily chemical field.

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