Skin repairing composition and preparation method thereof

Abstract

The invention belongs to the technical field of skin repair, and particularly relates to a skin repair composition and a preparation method thereof.The skin repair composition is characterized by being prepared from carbomer, stearic acid, VitE, hydrolyzed pearl, bisabolol, olive oil emulsifying wax, glycerin, centella asiatica extract, vaseline, ethylparaben, methylparaben and water according to the mass ratio of (0.1-0.5): (1-10): (1-5): (1-5): (0.1-0.5): (1-5): (1-10): (0.1-1): (1-10): (0.1-1): (0.1-1): 1. The composition has the effects of repairing skin barriers and reducing redness, the skin irritation degree caused by external irritation is relieved, and the good soothing and repairing effects are achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of skin repair, and in particular to a skin repair composition and a preparation method thereof. Background Art

[0002] Sun exposure and climate change are unavoidable factors in our daily lives. As we age, our skin loses moisture and collagen, which can cause varying degrees of damage to the skin. For example, long-term ultraviolet radiation can cause solar keratinization of the skin, which may cause erythema and peeling. It can also cause aging of collagen fibers in the dermis, resulting in spots and wrinkles. In recent years, due to the development of industrial economic activities, land erosion and desertification, extreme weather has become frequent, and the skin is often in a state of alternating cold and warm, causing skin sensitivity. It is important to use skin care products to help the skin repair and restore the skin barrier.

[0003] With the improvement of living standards, cosmetics have become an indispensable daily necessity, and people's requirements for cosmetics are constantly increasing. There are many types of skin repair skin care products on the market, but the effects are not satisfactory and the resulting effects are single. Most products directly add a large amount of synthetic reagents to meet the needs. While repairing the skin, they also cause other problems such as skin sensitivity, redness, dullness, etc., which makes it difficult to meet people's growing demand for high-quality skin care products. In summary, the existing repair products have the problem of causing skin sensitivity and redness. Summary of the invention

[0004] The purpose of the present invention is to provide a composition with skin repairing effect to solve the problem of allergic redness.

[0005] A skin repairing composition is prepared from carbomer, stearic acid, VitE, hydrolyzed pearls, bisabolol, olive oil emulsified wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben and water in a mass ratio of 0.1-0.5:1-10:1-5:1-5:0.1-0.5:1-5:1-10:0.1-1:1-10:0.1-1:0.1-1:1.

[0006] Preferably, the mass ratio of carbomer, stearic acid, VitE, hydrolyzed pearls, bisabolol, olive oil emulsified wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water is 0.2-0.3:4-8:2-3:2-3:0.2-0.3:2-3:3-7:0.3-0.6:4-8:0.3-0.6:0.4-0.7:1.

[0007] The method for preparing the skin repairing composition comprises the following steps:

[0008] Weigh: weigh carbomer, stearic acid, VitE, hydrolyzed pearls, bisabolol, olive oil emulsified wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water according to the mass ratio of 0.1-0.5:1-10:1-5:1-5:0.1-0.5:1-5:1-10:0.1-1:1-10:0.1-1:0.1-1;

[0009] First mixing: mix the weighed water, glycerin, Centella asiatica extract, bisabolol, carbomer, hydrolyzed pearls, ethylparaben and methylparaben, and heat for the first time to obtain mixture 1;

[0010] Second mixing: Stir and mix the weighed stearic acid, VitE, vaseline, and olive oil emulsified wax, stir and homogenize after mixing, and heat for the second time and then stir and homogenize for the first time to obtain mixture 2; it plays a role in reducing the size of the dispersed matter and improving the uniformity of the dispersed matter distribution

[0011] Homogenization and emulsification: Add mixture 1 to mixture 2, heat for the third time, and stir for the second time to homogenize;

[0012] After emulsification, stir when the temperature drops to 35℃~40℃;

[0013] The temperature of the first heating is higher than the temperature of the third heating, and the temperature of the third heating is higher than the temperature of the second heating;

[0014] The homogenizing speed during the second stirring and homogenizing is greater than the homogenizing speed during the first stirring and homogenizing.

[0015] Preferably, the temperature of the first heating is 85°C to 95°C.

[0016] Preferably, the temperature is maintained at 85°C to 95°C for 10 to 20 minutes.

[0017] Preferably, the temperature of the second heating is 40°C to 50°C.

[0018] Preferably, the temperature of the third heating is 60°C to 80°C.

[0019] Preferably, during the first stirring and homogenizing, the homogenizing speed is 1200 rpm to 1500 rpm, the stirring speed is 30 rpm to 40 rpm, and the time is 3 min to 5 min.

[0020] Preferably, during the second stirring and homogenizing, the homogenizing speed is 2000 rpm to 2500 rpm, the stirring speed is 30 rpm to 40 rpm, and the time is 10 min to 15 min.

[0021] Preferably, when the temperature drops to 35° C. to 40° C., the stirring speed is 20 rpm to 25 rpm, and the stirring time is 15 min to 20 min.

[0022] Hydrolyzed pearls can soothe and tighten the skin, improve skin condition, improve skin roughness and aging, and effectively remove wrinkles. They are anti-inflammatory and antioxidant, improve skin cell activity, regulate skin moisture balance, and inhibit melanin production. They are safe and efficient whitening active substances. Hydrolyzed pearls have a strong ability to scavenge DPPH and ABTS free radicals.

[0023] Bisabolol is one of the main active ingredients of chamomile, a plant of the Asteraceae family. It has multiple biological properties such as antibacterial and antioxidant properties. It has obvious anti-inflammatory, relieving and anti-allergic functions, and has skin care effects on allergic skin or children's skin.

[0024] Centella asiatica extract can inhibit the expression of transforming growth factor, reduce the release of mast cell-dependent inflammatory mediators, and has an anti-allergic effect. It can also downregulate the level of inflammatory factors, inhibit skin inflammatory reactions, and play a positive role in the treatment of inflammatory reactive skin. In addition, Centella asiatica extract also has the effects of anti-oxidation, promoting epidermal regeneration, promoting wound healing, inhibiting melanin activity, and regulating cortical metabolism.

[0025] Stearic acid has moisturizing, antibacterial and anti-inflammatory, and anti-aging effects. It has antibacterial and anti-inflammatory effects, can reduce bacteria on the skin surface, and relieve skin inflammation to a certain extent. It can also be antioxidant, can reduce skin free radical damage, prevent premature wrinkles on the skin, and slow down the aging process.

[0026] Vaseline can relieve redness, swelling, itching, moisturize, protect the skin and replenish water. It forms a protective film locally to prevent the body's moisture from evaporating, which has the benefit of moisturizing the skin. Vaseline has a certain anti-inflammatory and bactericidal effect. After application, it can inhibit the reproduction and proliferation of local bacteria, thereby improving the symptoms of discomfort.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] The skin repair composition provided in the present application has the effects of repairing the skin barrier, reducing moisture loss of the skin, reducing redness, and can also reduce the degree of skin irritation caused by external stimuli, achieving a good soothing and repairing effect.

[0029] The composition of the present invention cooperates with each other, and the hydrolyzed pearls, bisabolol, Centella asiatica extract, Centella asiatica extract, and stearic acid cooperate synergistically. Although each raw material has its own effect, when the synergistic effect of the four disappears, even the lack of any one raw material will cause a significant decrease in the effect, and when these raw materials are compounded with other commonly used cosmetic raw materials, there is no significant effect of reducing the degree of skin irritation caused by external stimuli, such as hyaluronic acid. Therefore, the composition of the present invention has a significant improvement.

[0030] The present invention dissolves bisabolol, hydrolyzed pearls, and Centella asiatica extract in a solvent, and the two are coordinated to significantly reduce the expression of inflammatory factors, reduce skin inflammation, soothe skin redness, and reduce skin burning and irritation, resist oxidation, promote epidermal regeneration, and effectively improve the scavenging effect of cells on scavenging DPPH free radicals, hydroxyl free radicals, superoxide anion free radicals, and ABTS free radicals. While resisting inflammation and oxidation, the skin surface can also be kept hydrated, and the production and accumulation of melanin can be reduced. DETAILED DESCRIPTION

[0031] The specific embodiments of the present invention are described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the protection scope of the present invention. The experimental methods described in the embodiments of the present invention are conventional methods unless otherwise specified.

[0032] Example 1

[0033] A skin repairing composition is prepared from the following raw materials in parts by weight: carbomer, stearic acid, VitE, hydrolyzed pearls, bisabolol, olive oil emulsified wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water;

[0034] The mass ratio of carbomer, stearic acid, VitE, hydrolyzed pearl, bisabolol, olive oil emulsifying wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water is 0.3:6:2:3:0.2:2:7:0.4:8:0.5:0.6:1.

[0035] A method for preparing a skin repairing composition comprises the following steps:

[0036] (1) Weigh: Weigh carbomer, stearic acid, Vitamin E, hydrolyzed pearls, bisabolol, olive oil emulsifying wax, glycerin, Centella asiatica extract, petrolatum, ethylparaben, methylparaben, and water in a mass ratio of 0.3:6:2:3:0.2:2:7:0.4:8:0.5:0.6:1.

[0037] (2) First mixing: Stir and dissolve the weighed water, glycerin, Centella asiatica extract, bisabolol, carbomer, hydrolyzed pearls, ethylparaben, and methylparaben, and heat to 90° C. and keep warm for 15 minutes to obtain a mixture 1.

[0038] (3) Second mixing: Mix the weighed stearic acid, VitE, vaseline and olive oil emulsified wax, heat to 50° C., and stir to homogenize at a stirring speed of 30 rpm and a homogenizing speed of 1500 rpm for 4 min to obtain a second mixture.

[0039] (4) Homogenization and emulsification: Add mixture 1 to mixture 2, heat to 80° C., and stir to homogenize at a stirring speed of 30 rpm and a homogenization speed of 2500 rpm for 15 minutes.

[0040] (5) After emulsification, when the temperature drops to 38°C, stirring is performed again at a speed of 20 rpm for 15 min.

[0041] Example 2

[0042] A skin repairing composition is prepared from the following raw materials in parts by weight: carbomer, stearic acid, VitE, hydrolyzed pearls, bisabolol, olive oil emulsified wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water;

[0043] The mass ratio of carbomer, stearic acid, VitE, hydrolyzed pearl, bisabolol, olive oil emulsifying wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water is 0.2:8:3:2:0.3:1:3:0.6:4:0.3:0.7:1.

[0044] A method for preparing a skin repairing composition comprises the following steps:

[0045] (1) Weigh: Weigh carbomer, stearic acid, Vitamin E, hydrolyzed pearls, bisabolol, olive oil emulsifying wax, glycerin, Centella asiatica extract, petrolatum, ethylparaben, methylparaben, and water in a mass ratio of 0.2:8:3:2:0.2:2:3:0.6:4:0.3:0.7:1.

[0046] (2) First mixing: Stir and dissolve the weighed water, glycerin, Centella asiatica extract, bisabolol, carbomer, hydrolyzed pearls, ethylparaben, and methylparaben, and heat to 85° C. and keep warm for 15 minutes to obtain a mixture 1.

[0047] (3) Second mixing: Mix the weighed stearic acid, VitE, vaseline and olive oil emulsified wax, heat to 40° C., and stir to homogenize at a stirring speed of 30 rpm and a homogenizing speed of 1300 rpm for 4 min to obtain a second mixture.

[0048] (4) Homogenization and emulsification: Add mixture 1 to mixture 2, heat to 60° C., and stir to homogenize at a stirring speed of 40 rpm and a homogenization speed of 2000 rpm for 10 minutes.

[0049] (5) After emulsification, when the temperature drops to 38°C, stirring is performed again at a speed of 20 rpm for 15 min.

[0050] Example 3

[0051] A skin repairing composition is prepared from the following raw materials in parts by weight: carbomer, stearic acid, VitE, hydrolyzed pearls, bisabolol, olive oil emulsified wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water;

[0052] The mass ratio of carbomer, stearic acid, VitE, hydrolyzed pearl, bisabolol, olive oil emulsifying wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water is 0.5:1:5:5:0.1:1:10:0.1:10:0.1:1:1.

[0053] A method for preparing a skin repairing composition comprises the following steps:

[0054] (1) Weigh: Weigh carbomer, stearic acid, Vitamin E, hydrolyzed pearls, bisabolol, olive oil emulsifying wax, glycerin, Centella asiatica extract, petrolatum, ethylparaben, methylparaben, and water in a mass ratio of 0.5:1:5:5:0.1:1:10:0.1:10:0.1:1:1.

[0055] (2) First mixing: Stir and dissolve the weighed water, glycerin, Centella asiatica extract, bisabolol, carbomer, hydrolyzed pearls, ethylparaben, and methylparaben, and heat to 95° C. and keep warm for 10 minutes to obtain a mixture 1.

[0056] (3) Second mixing: Mix the weighed stearic acid, VitE, vaseline and olive oil emulsified wax, heat to 45° C., and stir to homogenize at a stirring speed of 30 rpm and a homogenizing speed of 1200 rpm for 4 min to obtain a second mixture.

[0057] (4) Homogenization and emulsification: Add mixture 1 to mixture 2, heat to 70° C., and stir to homogenize at a stirring speed of 30 rpm and a homogenization speed of 2500 rpm for 10 minutes.

[0058] (5) After emulsification, when the temperature drops to 38°C, stirring is performed again at a speed of 25 rpm for 20 min.

[0059] Example 4

[0060] A skin repairing composition is prepared from the following raw materials in parts by weight: carbomer, stearic acid, VitE, hydrolyzed pearls, bisabolol, olive oil emulsified wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water;

[0061] The mass ratio of carbomer, stearic acid, VitE, hydrolyzed pearl, bisabolol, olive oil emulsifying wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water is 0.1:10:1:1:0.5:5:1:1:1:1:0.1:1.

[0062] A method for preparing a skin repairing composition comprises the following steps:

[0063] (1) Weighing: Weigh carbomer, stearic acid, Vitamin E, hydrolyzed pearls, bisabolol, olive oil emulsifying wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water in a mass ratio of 0.1:10:1:1:0.5:5:1:1:1:1:0.1:1.

[0064] (2) First mixing: Stir and dissolve the weighed water, glycerin, Centella asiatica extract, bisabolol, carbomer, hydrolyzed pearls, ethylparaben, and methylparaben, and heat to 90° C. and keep warm for 20 minutes to obtain a mixture 1.

[0065] (3) Second mixing: Mix the weighed stearic acid, VitE, vaseline and olive oil emulsified wax, heat to 50° C., and stir to homogenize at a stirring speed of 30 rpm and a homogenizing speed of 1500 rpm for 4 min to obtain a second mixture.

[0066] (4) Homogenization and emulsification: Add mixture 1 to mixture 2, heat to 80° C., and stir to homogenize at a stirring speed of 30 rpm and a homogenization speed of 2000 rpm for 15 minutes.

[0067] (5) After emulsification, when the temperature drops to 38°C, stirring is performed again at a speed of 20 rpm for 15 min.

[0068] Comparative Example 1

[0069] The difference between Comparative Example 1 and Example 1 is that the raw materials do not contain hydrolyzed pearls, as follows:

[0070] A skin repairing composition is prepared from the following raw materials in parts by weight: carbomer, stearic acid, VitE, bisabolol, olive oil emulsifying wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water;

[0071] The mass ratio of carbomer, stearic acid, VitE, bisabolol, olive oil emulsifying wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben and water is 0.3:6:2:0.2:2:7:0.4:8:0.5:0.6:1.

[0072] A method for preparing a skin repairing composition comprises the following steps:

[0073] (1) Weigh: Weigh carbomer, stearic acid, Vitamin E, hydrolyzed pearls, bisabolol, olive oil emulsifying wax, glycerin, Centella asiatica extract, petrolatum, ethylparaben, methylparaben, and water in a mass ratio of 0.3:6:2:0.2:2:7:0.4:8:0.5:0.6:1.

[0074] (2) First mixing: Stir and dissolve the weighed water, glycerin, Centella asiatica extract, bisabolol, carbomer, ethylparaben and methylparaben, and heat to 90° C. for 15 minutes to obtain a mixture 1.

[0075] (3) Second mixing: Mix the weighed stearic acid, VitE, vaseline and olive oil emulsified wax, heat to 50° C., and stir to homogenize at a stirring speed of 30 rpm and a homogenizing speed of 1500 rpm for 4 min to obtain a second mixture.

[0076] (4) Homogenization and emulsification: Add mixture 1 to mixture 2, heat to 80° C., and stir to homogenize at a stirring speed of 30 rpm and a homogenization speed of 2500 rpm for 15 minutes.

[0077] (5) After emulsification, when the temperature drops to 38°C, stirring is performed again at a speed of 20 rpm for 15 min.

[0078] Comparative Example 2

[0079] The difference between Comparative Example 2 and Example 1 is that the raw materials do not contain bisabolol, as follows:

[0080] A skin repairing composition is prepared from the following raw materials in parts by weight: carbomer, stearic acid, VitE, hydrolyzed pearls, olive oil emulsified wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water;

[0081] The mass ratio of carbomer, stearic acid, VitE, hydrolyzed pearl, olive oil emulsified wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water is 0.3:6:2:3:2:7:0.4:8:0.5:0.6:1.

[0082] A method for preparing a skin repairing composition comprises the following steps:

[0083] (1) Weigh: Weigh carbomer, stearic acid, Vitamin E, hydrolyzed pearls, bisabolol, olive oil emulsifying wax, glycerin, Centella asiatica extract, petrolatum, ethylparaben, methylparaben, and water in a mass ratio of 0.3:6:2:3:2:7:0.4:8:0.5:0.6:1.

[0084] (2) First mixing: Stir and dissolve the weighed water, glycerin, Centella asiatica extract, carbomer, hydrolyzed pearls, ethylparaben, and methylparaben, and heat to 90° C. and keep warm for 15 minutes to obtain a mixture 1.

[0085] (3) Second mixing: Mix the weighed stearic acid, VitE, vaseline and olive oil emulsified wax, heat to 50° C., and stir to homogenize at a stirring speed of 30 rpm and a homogenizing speed of 1500 rpm for 4 min to obtain a second mixture.

[0086] (4) Homogenization and emulsification: Add mixture 1 to mixture 2, heat to 80° C., and stir to homogenize at a stirring speed of 30 rpm and a homogenization speed of 2500 rpm for 15 minutes.

[0087] (5) After emulsification, when the temperature drops to 38°C, stirring is performed again at a speed of 20 rpm for 15 min.

[0088] Comparative Example 3

[0089] The difference between Comparative Example 3 and Example 1 is that the raw materials do not contain Centella asiatica extract, which is as follows:

[0090] A skin repairing composition is prepared from the following raw materials in parts by weight: carbomer, stearic acid, VitE, hydrolyzed pearls, bisabolol, olive oil emulsified wax, glycerin, vaseline, ethylparaben, methylparaben, and water;

[0091] The mass ratio of carbomer, stearic acid, VitE, hydrolyzed pearl, bisabolol, olive oil emulsifying wax, glycerin, vaseline, ethylparaben, methylparaben and water is 0.3:6:2:3:0.2:2:7:8:0.5:0.6:1.

[0092] A method for preparing a skin repairing composition comprises the following steps:

[0093] (1) Weigh: Weigh carbomer, stearic acid, Vitamin E, hydrolyzed pearls, bisabolol, olive oil emulsifying wax, glycerin, Centella asiatica extract, petrolatum, ethylparaben, methylparaben, and water in a mass ratio of 0.3:6:2:3:0.2:2:7:8:0.5:0.6:1.

[0094] (2) First mixing: Stir and dissolve the weighed water, glycerin, bisabolol, carbomer, hydrolyzed pearls, ethylparaben, and methylparaben, and heat to 90° C. and keep warm for 15 minutes to obtain a mixture 1.

[0095] (3) Second mixing: Mix the weighed stearic acid, VitE, vaseline and olive oil emulsified wax, heat to 50° C., and stir to homogenize at a stirring speed of 30 rpm and a homogenizing speed of 1500 rpm for 4 min to obtain a second mixture.

[0096] (4) Homogenization and emulsification: Add mixture 1 to mixture 2, heat to 80° C., and stir to homogenize at a stirring speed of 30 rpm and a homogenization speed of 2500 rpm for 15 minutes.

[0097] (5) After emulsification, when the temperature drops to 38°C, stirring is performed again at a speed of 20 rpm for 15 min.

[0098] Comparative Example 4

[0099] The difference between Comparative Example 4 and Example 1 is that the raw material does not contain stearic acid, as follows:

[0100] A skin repairing composition is prepared from the following raw materials in parts by weight: carbomer, VitE, hydrolyzed pearls, bisabolol, olive oil emulsified wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water;

[0101] The mass ratio of carbomer, VitE, hydrolyzed pearl, bisabolol, olive oil emulsified wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water is 0.3:2:3:0.2:2:7:0.4:8:0.5:0.6:1.

[0102] A method for preparing a skin repairing composition comprises the following steps:

[0103] (1) Weigh: Weigh carbomer, VitE, hydrolyzed pearls, bisabolol, olive oil emulsified wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water in a mass ratio of 0.3:2:3:0.2:2:7:0.4:8:0.5:0.6:1.

[0104] (2) First mixing: Stir and dissolve the weighed water, glycerin, Centella asiatica extract, bisabolol, carbomer, hydrolyzed pearls, ethylparaben, and methylparaben, and heat to 90° C. and keep warm for 15 minutes to obtain a mixture 1.

[0105] (3) Second mixing: Mix the weighed VitE, vaseline and olive oil emulsified wax, heat to 50° C., and stir to homogenize at a stirring speed of 30 rpm and a homogenizing speed of 1500 rpm for 4 min to obtain a second mixture.

[0106] (4) Homogenization and emulsification: Add mixture 1 to mixture 2, heat to 80° C., and stir to homogenize at a stirring speed of 30 rpm and a homogenization speed of 2500 rpm for 15 minutes.

[0107] (5) After emulsification, when the temperature drops to 38°C, stirring is performed again at a speed of 20 rpm for 15 min.

[0108] Efficacy Experiment

[0109] 1. Human skin fibroblast HSF proliferation assay (CCK-8 method)

[0110] The activity of human skin fibroblasts was determined by CCK-8. HSF was cultured at 5 × 10 3 The cells were inoculated at a density of 10 cells / well in a 96-well culture plate, 90 μL was inoculated in each well, and cultured at 37° C., 5% CO2 for 24 h. After the cells adhered to the wall, they were taken out and divided into an experimental group, a negative control group (containing an equal amount of cell resuspension) and a blank group (containing an equal amount of DMEM culture medium). 10 μL / well of the skin repair composition of Examples 1-4 of the present invention was added to the experimental group, and an equal amount of DMEM culture medium was added to the negative control group. The cells were cultured in a standard incubator and taken out after 48 h. Subsequently, 10 μL of CCK-8 buffer was added to each well, and the plates were placed in an incubator and incubated in the dark for 1 h. The absorbance (OD) of each well was detected at a wavelength of 450 nm using an enzyme reader.

[0111] Calculate the cell proliferation rate (%) = (experimental group OD-blank group OD) / (negative control group OD-blank control group OD) x 100%. The results are shown in Table 1 below.

[0112] Table 1 Cell proliferation rate of the examples

[0113]

[0114] As shown in Table 1, the calculated cell proliferation rate was 97.2%.

[0115] The cell proliferation rates of the comparative examples are shown in Table 2.

[0116] Table 2 Cell proliferation rate of comparative example

[0117]

[0118] 2. Antioxidant Test

[0119] 1. Hydroxyl free radical scavenging experiment

[0120] Using Fenton reaction to generate hydroxyl radicals: H2O2+Fe 2+ = OH + H2O + Fe 3+ , salicylic acid is added to the reaction system, and the hydroxyl radicals generated by the Fenton reaction will react with salicylic acid to generate 2,3-dihydroxybenzoic acid with maximum absorbance at 510nm. Adding a test substance with the function of scavenging hydroxyl radicals to the reaction system will reduce the generation of corresponding colored compounds. The scavenging effect can be determined by measuring the absorbance of the reaction solution containing the test substance at 510nm and comparing it with the blank solution using the fixed reaction time method. The results are shown in Table 3.

[0121] 2. DPPH free radical scavenging rate

[0122] The reaction system comprises 0.1 mL of any composition obtained in Examples 1-4 or any composition obtained in Comparative Examples 1-4, 2 mL of 0.05 M acetate buffer solution, 1.9 mL of anhydrous ethanol, and 1 mL of 0.5 M DPPH ethanol solution, wherein the DPPH ethanol solution is added last. The reaction system is protected from light for 30 minutes, and finally detected at 517 nm. The results are shown in Table 3.

[0123] Clearance rate (%) = (Ab-(Abs-As)) / Ab*100%

[0124] Ab: Replace the sample solution with water

[0125] Abs: add sample and DPPH

[0126] As: add sample, DPPH is replaced by anhydrous ethanol

[0127] 3. ABTS free radical scavenging experiment

[0128] Accurately measure 3 ml of ABTS+ working solution, then use a pipette to accurately transfer 50 μL of sample solution, shake for 30 seconds to mix it thoroughly, and then let the mixed solution stand for 6 minutes at room temperature and in the dark, and then quickly use a UV spectrophotometer to measure the absorbance of the solution at 734 nm, recorded as Ai. Use 50 μL of methanol to replace the sample solution, and measure the absorbance at 734 nm as a blank control, recorded as A0, and use 3 mL of anhydrous ethanol to replace the ABTS+ working solution, and measure the absorbance of the sample background at 734 nm, recorded as Aj, and calculate the scavenging rate K2 of the sample solution for ABTS+ free radicals. The results are shown in Table 3.

[0129]

[0130] Table 3 Antioxidant test results

[0131]

[0132] 3. Test Experiment

[0133] 58 volunteers aged 30-46 with dark and sensitive faces were selected and randomly divided into 2 groups, 29 in each group, and the compositions of the experimental example and the control example were applied to the entire face with a thickness of about 0.5 mm, once in the morning and once in the evening every day for 21 days; the volunteers were tested in test examples 1-2.

[0134] Test Example 1: Moisture Test

[0135] After the volunteers washed their faces on the 0th and 21st days, they waited for 10 minutes at an ambient temperature of 23±1°C and a humidity of 50±10%. The German CKMoistureMap MM200 skin moisture distribution tester was used to test the moisture content of the volunteers' faces. Specifically, the values ​​of the same 8 places on the faces of different volunteers were taken, and the average value was taken. The data on the 0th day was used as the benchmark, and the data on the 21st day was compared with it to reflect the changes in the data before and after use;

[0136] Moisture content change = (moisture content on day 21 - moisture content on day 0) / moisture content on day 21;

[0137] The average value of the indicator data changes of 58 people is taken as the final result, and the final result is shown in Table 3.

[0138] Test Example 2: Sensitivity Test

[0139] (1) On day 0 and day 21, after washing their faces, volunteers rated their facial sensitivity (0-3 points) in an environment with a temperature of 23±1°C and a humidity of 50±10%;

[0140] Sensitivity scoring criteria: 2-3 points (no sensitivity); 1-2 points (mild sensitivity); 0-1 points (moderate sensitivity); 0 points (severe sensitivity); the average score of 29 people in each group was taken as the final result, and the results are shown in Table 1.

[0141] (2) On the 0th and 21st days, after washing their faces, the volunteers were placed in an environment with a temperature of 23±1°C and a humidity of 50±10% for 10 minutes. Then, photos were taken using the MC-880 five-spectrum skin detector. The same dermatologist evaluated the improvement in facial skin pigments, dark spots, acne, pores, and wrinkles for each volunteer before and after use. The average of the changes in the indicator data of the 29 people in each group was taken as the final result.

[0142] Scoring criteria: 0 points (normal, no improvement in facial skin surface, dark spots, acne, pores, and wrinkles); 0-1 points (mild, slight improvement in facial skin surface, dark spots, acne, pores, and wrinkles); 1-2 points (moderate, good improvement in facial skin surface, dark spots, acne, pores, and wrinkles); 2-3 points (severe, obvious improvement in facial skin surface, dark spots, acne, pores, and wrinkles); the results are shown in Table 3.

[0143] Table 3 Test results

[0144]

[0145]

[0146] It should be noted that when the claims of the present invention involve numerical ranges, it should be understood that the two endpoints of each numerical range and any numerical value between the two endpoints can be selected. In order to avoid redundancy, the present invention describes a preferred embodiment.

[0147] Although the preferred embodiments of the present invention have been described, those skilled in the art may make other changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0148] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A composition for skin repair, characterized in that: The invention is prepared from carbomer, stearic acid, VitE, hydrolyzed pearl, bisabolol, olive oil emulsified wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben and water in a mass ratio of 0.1-0.5:1-10:1-5:1-5:0.1-0.5:1-5:1-10:0.1-1:1-10:0.1-1:0.1-1:

1.

2. The composition according to claim 1, characterized in that The mass ratio of carbomer, stearic acid, VitE, hydrolyzed pearl, bisabolol, olive oil emulsified wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben and water is 0.2-0.3:4-8:2-3:2-3:0.2-0.3:2-3:3-7:0.3-0.6:4-8:0.3-0.6:0.4-0.7:

1.

3. The method for preparing a skin repairing composition according to claim 1, characterized in that: The following steps are involved: Weigh: weigh carbomer, stearic acid, VitE, hydrolyzed pearls, bisabolol, olive oil emulsified wax, glycerin, Centella asiatica extract, vaseline, ethylparaben, methylparaben, and water according to the mass ratio of 0.1-0.5:1-10:1-5:1-5:0.1-0.5:1-5:1-10:0.1-1:1-10:0.1-1:0.1-1; First mixing: mix the weighed water, glycerin, Centella asiatica extract, bisabolol, carbomer, hydrolyzed pearls, ethylparaben and methylparaben, and heat for the first time to obtain mixture 1; Second mixing: Stir and mix the weighed stearic acid, VitE, vaseline and olive oil emulsified wax, stir and homogenize after mixing, and heat for the second time and then stir and homogenize for the first time to obtain a second mixture; Homogenization and emulsification: Add mixture 1 to mixture 2, heat for the third time, and stir for the second time to homogenize; After emulsification, stir when the temperature drops to 35℃~40℃; The temperature of the first heating is higher than the temperature of the third heating, and the temperature of the third heating is higher than the temperature of the second heating; The homogenizing speed during the second stirring and homogenizing is greater than the homogenizing speed during the first stirring and homogenizing.

4. The preparation method according to claim 3, characterized in that: The temperature of the first heating is 85°C to 95°C.

5. The preparation method according to claim 4, characterized in that: Keep at 85℃~95℃ for 10min~20min.

6. The preparation method according to claim 3, characterized in that: The temperature of the second heating is 40°C to 50°C.

7. The preparation method according to claim 3, characterized in that: The temperature of the third heating is 60°C to 80°C.

8. The preparation method according to claim 3, characterized in that: When stirring and homogenizing for the first time, the homogenizing speed is 1200 rpm to 1500 rpm, the stirring speed is 30 rpm to 40 rpm, and the time is 3 min to 5 min.

9. The preparation method according to claim 3, characterized in that: During the second stirring and homogenizing, the homogenizing speed is 2000 rpm to 2500 rpm, the stirring speed is 30 rpm to 40 rpm, and the time is 10 min to 15 min.

10. The preparation method according to claim 3, characterized in that: When the temperature drops to 35°C to 40°C, the stirring speed is 20 rpm to 25 rpm, and the stirring time is 15 min to 20 min.

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