Organic material deodorization leavening agent and preparation method thereof

Abstract

The invention relates to the technical field of environmental engineering, and discloses an organic material deodorization leavening agent and a preparation method thereof, and the preparation method comprises the following preparation steps: step 1, preparation work; step 2, preparing a microbial engineering and photoresponse material; step 3, microbial inoculation and culture; step 4, fermentation process control and optimization; and step 5, harvesting and drying a product, selecting a microbial raw material which has relatively strong metabolic activity and can degrade odor, preparing a photosensitive enzyme, a photosensitive polymer and photosensitive algae as photosensitive materials, mixing the photosensitive materials with a microbial culture medium, and changing a metabolic pathway of microorganisms by adjusting light response activity. The mixing proportion of the photosensitive enzyme and the microbial culture medium can significantly improve the metabolic activity of microorganisms under ultraviolet irradiation, thereby accelerating the degradation process of odor. By optimizing the ratio of the photosensitive material to the culture medium, the decomposition capability of microorganisms on harmful substances can be enhanced, and the effect of the organic fertilizer deodorization leavening agent is remarkably improved.

Description

Technical Field

[0001] The invention relates to the technical field of environmental engineering, and in particular to an organic material deodorizing fermentation agent and a preparation method thereof. Background Art

[0002] With the acceleration of urbanization and the development of industrialized agriculture, the use of organic fertilizers is becoming increasingly popular. However, the fermentation process of organic fertilizers is often accompanied by the generation of a large amount of odor, which not only pollutes the environment, but also may have a negative impact on human health and the quality of life of surrounding residents.

[0003] Traditional odor control methods, such as physical adsorption and chemical adsorption, can effectively remove odor, but they have problems such as high cost, low efficiency, and secondary pollution to the environment.

[0004] In recent years, the application of microbial fermentation technology in organic waste treatment has received extensive attention. Microorganisms can effectively degrade odor molecules through their metabolism, achieving the effect of purifying the air and improving the environment.

[0005] At present, the odor degradation rate of microbial fermentation technology is relatively slow. The degradation rate of most microorganisms under normal conditions cannot meet the needs of large-scale organic waste treatment, especially in the large-scale organic fertilizer fermentation process, where the amount of odor generated is huge, and the degradation capacity of a single microorganism is often unable to quickly and efficiently cope with the large amount of odor release.

[0006] For this reason, we propose a kind of organic material deodorizing fermentation agent and preparation method thereof. Summary of the invention

[0007] The present invention mainly solves the above technical problems and provides an organic material deodorizing fermentation agent and a preparation method thereof.

[0008] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme, a deodorizing fermentation agent for organic materials, characterized in that it includes microbial raw materials, light-responsive materials and culture medium components, the microbial raw materials include a weight ratio of 0.5% to 2% of a complex bacillus group, 0.5% to 2% of a lactic acid bacteria group, 0.5% to 1% of a yeast group, 0.5% to 2% of a nitrifying bacteria, 0.5% to 1.5% of an actinomycete group, 0.5% to 1% of an anaerobic bacteria group, and 0.5% to 1% of a phosphate-solubilizing bacteria; the light-responsive material includes a weight ratio of 0.001%-0.1% of a photosensitizer, 1%-5% of a photosensitive polymer, and 0.1%-1% of a photosensitive algae; the culture medium components include a carbon source with a concentration range of 1%-3% (w / v) and a nitrogen source with a concentration range of 0.1%-1% (w / v).

[0009] A method for preparing an organic material deodorizing fermentation agent comprises the following preparation steps: Step 1: Preparation; Step 2: Microbial engineering and preparation of light-responsive materials; Step 3: Microbial inoculation and cultivation; Step 4: Fermentation process control and optimization; Step 5: Product harvesting and drying. Select microbial raw materials with strong metabolic activity and the ability to degrade odor, enhance their responsiveness to light activation through genetic engineering, prepare photosensitive enzymes, photosensitive polymers and photosensitive algae as photosensitive materials, mix the photosensitive materials with the microbial culture medium, and change the metabolic pathway of the microorganisms by adjusting the light-responsive activity.

[0010] Preferably, in step 2, a microbial community with strong metabolic activity and capable of degrading odor is selected, including a complex bacillus community, a lactic acid bacteria community, a yeast community, a nitrifying bacteria community, an actinomycete community, an anaerobic bacteria community and a mixture of phosphate-solubilizing bacteria, and its responsiveness to light activation is enhanced by genetic engineering, and a photosensitive material is prepared, including a photosensitive enzyme with a concentration of 0.001%-0.1%, a photosensitive polymer with a concentration of 1%-5%, and a photosensitive algae with a concentration of 0.1%-1%, and a microbial culture medium is prepared, including a carbon source with a concentration range of 1%-3% (w / v), and a nitrogen source with a concentration range of 0.1%-1% (w / v). By combining photosensitive materials with microbial flora, their light response activity can be adjusted under the irradiation of specific light sources, thereby changing the metabolic pathway of the microorganisms.

[0011] Preferably, in step 2, the mixing ratio of the photosensitive material and the microbial culture medium is obtained through a mixing experiment to obtain an optimal mixing ratio, and the mixing experiment steps include: S1: Mix the photosensitive material with the culture medium, prepare the photosensitive enzyme, photosensitive polymer and photosensitive algae solution according to the predetermined concentration, adjust the ratio to ensure that each photosensitive material is fully dissolved or evenly dispersed, prepare the culture medium and add appropriate concentrations of carbon source (1%-3%) and nitrogen source (0.1%-1%) according to the needs of the selected microorganisms, and adjust the culture medium composition according to the characteristics of different strains to meet their nutritional needs; S2: Mix the photosensitizing material and the culture medium, set the ratio, mix according to different mass ratios of the photosensitizing material and the culture medium, select several different ratios for experiments, 1:10 (photosensitive material: culture medium), 1:50 (photosensitive material: culture medium), 1:100 (photosensitive material: culture medium), and fully mix the photosensitizing material solution and the culture medium.

[0012] Preferably, the step 2, the mixing experiment step further comprises: S3: Experimental group and control group were set up. One experimental group was set up for each mixing ratio to ensure that the mixing ratio of the photosensitive material and the culture medium in each group was consistent. A control group without photosensitive material was set up. The experimental results showed that the photosensitive enzyme showed a significant increase in metabolic activity, significant changes in acid content and stable gas production within the concentration range of 0.001%-0.1% (w / v) at a mixing ratio of 1:50, making it the best photosensitive material; S4: Setting of lighting conditions: According to the response characteristics of the photosensitive material, select the appropriate light source, set the irradiation intensity and irradiation time of the light source, and adjust them according to the reactions of different experimental groups. By comparing the fermentation effects of different light sources (ultraviolet light, visible light and blue light) on the mixture ratio of photosensitive enzyme and culture medium of 1:50, the selection of light source and light intensity can be optimized.

[0013] Preferably, the step 2 includes a lighting experiment, and the lighting condition setting is determined by the lighting experiment. The lighting experiment step includes: S10: Prepare experimental materials; S20: Light source preparation; S30: medium inoculation; S40: Lighting condition setting; S50: Metabolite detection.

[0014] Preferably, in the illumination experiment, according to the optimal mixing ratio of 1:50, a photosensitive enzyme solution and a microbial culture medium are prepared, and appropriate concentrations of carbon source (1%-3%) and nitrogen source (0.1%-1%) are added to ensure that the culture medium meets the growth requirements of the microorganisms. The photosensitive enzyme solution and the culture medium are mixed in a mass ratio of 1:50. Three light sources are selected in the experiment, including ultraviolet light, visible light and blue light, and the light intensities are set to 10 μmol / m² / s, 50μmol / m² / s and 20μmol / m² / s, respectively.

[0015] Preferably, in the illumination experiment, the experimental group places the culture dish or culture bottle in different illumination environments, and the ultraviolet light, visible light and blue light correspond to different illumination intensities, respectively, and the control group is placed in a no-light environment as a basal metabolic control to ensure the comparison of illumination effects, metabolite detection and result analysis The experimental results show that ultraviolet light has a significant effect on the increase in acid content, can effectively activate photosensitive enzymes and promote degradation reactions, and enhance the removal of harmful odors. Although visible light has a more balanced effect, with both acid and gas production increasing, blue light has little effect on metabolic reactions and does not significantly promote changes in acid and gas production. The no-light group showed a slowed or incomplete metabolic reaction, indicating the importance of light in this experiment.

[0016] Preferably, in step three, a liquid culture medium suitable for the growth of microorganisms is prepared, and a carbon source, a nitrogen source and trace elements are added to support the growth of microorganisms such as complex Bacillus groups and lactic acid bacteria groups, the microorganisms are evenly inoculated into the culture medium according to a preset strain ratio, and according to the mixing ratio of the photosensitizer and the culture medium determined in step two, ultraviolet irradiation conditions are used to activate the photosensitizer to promote metabolic changes in the microorganisms.

[0017] Preferably, in step 4, the oxygen supply is adjusted according to the type of microorganism to ensure the growth requirements of aerobic and anaerobic bacteria, the pH value and metabolites in the fermentation liquid are regularly tested, and the fermentation process is optimized according to the test results.

[0018] The present invention provides an organic material deodorizing fermentation agent and a preparation method thereof. It has the following beneficial effects: (1) The organic material deodorizing fermentation agent and its preparation method select strains with strong metabolic ability under specific conditions, including complex Bacillus group, lactic acid bacteria group, yeast group, nitrifying bacteria, actinomycetes group, anaerobic bacteria group, and phosphate-solubilizing bacteria. The above microorganisms can more effectively decompose odor molecules under light conditions. By combining photosensitive materials with microorganisms, the mixing ratio of photosensitizer and microbial culture medium (such as 1:50) will affect the metabolic rate of microorganisms, ensuring that the metabolic activity of microorganisms is improved under ultraviolet irradiation, thereby accelerating the degradation process of odor. By improving the metabolic activity of microorganisms, the decomposition rate of harmful substances is significantly increased, thereby enhancing the deodorizing effect of the fermentation process.

[0019] (2) This kind of organic material deodorizing fermentation agent and its preparation method, through the experimental verification of mixing photosensitive materials with microbial culture medium, optimizes the light response ability in the fermentation process and enhances the metabolic activity of microorganisms. The experimental results show that at a mixing ratio of 1:50, the metabolic activity of the photosensitizer is significantly enhanced, the acid content changes significantly, and the gas production remains stable, indicating that the photosensitizer can exert the best light response effect at this ratio, ensuring that the interaction between the photosensitive material and the microorganism can effectively enhance the degradation ability of the microorganism, thereby improving the odor removal effect.

[0020] (3) This kind of organic material deodorizing fermentation agent and its preparation method, through irradiation experiments with different light sources, effectively evaluates the effects of different lighting conditions on microbial metabolic activity and fermentation effect. The experimental results show that ultraviolet light source exhibits the most significant effect at an intensity of 10μmol / m² / s, especially in terms of the increase in acid content, indicating that ultraviolet light can significantly activate photosensitive enzymes and promote their degradation reactions, thereby enhancing the generation of acidic metabolites and effectively removing harmful odors. By selecting an appropriate light source, the odor removal effect during the fermentation process can be further improved.

[0021] (4) The organic material deodorizing fermentation agent and its preparation method are applicable to organic waste treatment scenarios such as feces fermentation organic fertilizer, sludge fermentation organic fertilizer, and organic compost. For example, the feces produced by farms (such as pigs, cattle, sheep, chickens, ducks, etc.) are fermented and the use of organic material deodorizing fermentation agents can effectively reduce odor and promote the production of organic fertilizers, air deodorization and odor control. The sludge produced by sewage treatment plants can be deodorized and fermented by adding the fermentation agent and converted into organic fertilizer. Agricultural waste or garden waste is composted and the use of this fermentation agent can effectively accelerate the composting process while suppressing the generation of odor.

[0022] (5) The organic material deodorizing fermentation agent proposed by the present invention has a wide range of applications and can be used in multiple fields, such as farms, slaughterhouses, feather processing plants, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the implementation of the present invention or the technical solution in the prior art, the following is a brief introduction to the drawings required for the implementation or the prior art description. Obviously, the drawings described below are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0024] The structures, proportions, sizes, etc. disclosed in this specification are only used to match the contents disclosed in the specification so as to facilitate understanding and reading by persons familiar with the technology. They are not used to limit the conditions under which the present invention can be implemented, and therefore have no substantial technical significance. Any structural modification, change in proportion or adjustment of size should still fall within the scope of the technical contents disclosed in the present invention without affecting the effects and purposes that can be achieved by the present invention.

[0025] Figure 1 This is a diagram showing the effect of the experimental group in which the ratio of the photosensitive enzyme to the culture medium is 1:10; Figure 2 This is a diagram showing the effect of the experimental group in which the ratio of the sensitive enzyme to the culture medium is 1:50; Figure 3 This is a diagram showing the effect of the experimental group in which the ratio of the photosensitive polymer to the culture medium is 1:10; Figure 4 This is a diagram showing the effect of the experimental group in which the ratio of the photosensitive polymer to the culture medium is 1:50; Figure 5 This is a diagram showing the effect of the experimental group in which the ratio of photosensitive algae to culture medium is 1:10; Figure 6 This is a diagram showing the effect of the experimental group in which the ratio of photosensitive algae to culture medium is 1:50; Figure 7This is a diagram showing the effects of different light sources on the mixing ratio of photosensitive enzyme to culture medium of 1:50; Figure 8 The present invention is a flow chart for preparing an organic material deodorizing fermentation agent. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] Embodiment 1: An organic material deodorizing fermentation agent, comprising microbial raw materials, light-responsive materials and culture medium components, wherein the specific microbial raw materials include a complex bacillus group, a lactic acid bacteria group, a yeast group, a nitrifying bacteria group, an actinomycete group, an anaerobic bacteria group and a phosphate-solubilizing bacteria group; Photoresponsive materials include photosensitive enzymes, photosensitive polymers, and photosensitive algae; The components of the culture medium include carbon source and nitrogen source. The carbon source provides the energy required by microorganisms, and the nitrogen source is a necessary substance for microorganisms to synthesize proteins, enzymes and other cell structures.

[0028] Embodiment 2: A method for preparing an organic material deodorizing fermentation agent, wherein the organic fertilizer deodorizing fermentation agent mainly comprises a composite bacillus group, a lactic acid bacteria group, a yeast group, a nitrifying bacteria group, an actinomycete group, a carbon source, an anaerobic bacteria group and a phosphate-solubilizing bacteria group, and is intended to effectively reduce the odor of the organic fertilizer and accelerate its fermentation process through the synergistic effect of beneficial microorganisms; Step 1: Preparation Raw material preparation and equipment cleaning and disinfection. All equipment that comes into contact with the fermentation agent (such as agitators, containers, metering equipment, etc.) must be thoroughly cleaned and disinfected to ensure that there is no external contamination.

[0029] Step 2: Microbial engineering and photoresponsive material preparation Microbial selection and cultivation: Select microbial raw materials with strong metabolic activity and the ability to degrade odor, including complex Bacillus groups, lactic acid bacteria groups, yeast groups, nitrifying bacteria, actinomycetes groups, anaerobic bacteria groups, and phosphate-solubilizing bacteria. Through genetic engineering directed evolution, improve their response to light activation.

[0030] Preparation of photosensitive materials: Photosensitive enzyme at a concentration of 0.001%-0.1% (w / v) Photopolymer concentration ranges from 1% to 5% (w / v) Concentration range is 0.1%-1% (w / v) photosensitive algae Light-responsive materials (such as photosensitive polymers, photosensitive algae, and photosensitive enzymes) combined with microbial flora can change the metabolic pathways of microorganisms under the irradiation of specific light sources; The photosensitive material is mixed with the microbial culture medium, and its light response activity is adjusted according to different light sources and the characteristics of the photosensitive material; Preparation of microbial culture media: Carbon source concentration ranges from 1% to 3% (w / v) Nitrogen source with a concentration range of 0.1%-1% (w / v) Mixing Experiment Steps: The ratio of mixing the photosensitive material with the microbial culture medium is generally 1:10 to 1:100 (mass ratio of the photosensitive material to the culture medium). The most suitable ratio is screened out. In order to screen out the best mixing ratio, the following method steps are used for verification: S1: Mixing photosensitive material with culture medium Prepare solutions of photosensitive enzyme, photosensitive polymer, and photosensitive algae according to predetermined concentrations, and the specific ratios can be adjusted based on their respective solubilities to ensure that each photosensitive material can be fully dissolved or evenly dispersed; Prepare the culture medium according to the needs of the selected microorganisms and add appropriate concentrations of carbon source (1%-3%) and nitrogen source (0.1%-1%). You can also adjust its composition according to the characteristics of different strains to ensure that their nutritional needs are met; S2: Mixing photosensitive materials and culture medium Ratio setting: Mix according to different mass ratios of photosensitizer and culture medium (1:10 to 1:100), and select several different ratios for experiments: 1:10 (photosensitive material: culture medium), 1:50 (photosensitive material: culture medium), 1:100 (photosensitive material: culture medium); Mix the photosensitive material solution with the culture medium and use a magnetic stirrer or oscillation to ensure a uniform solution; S3: Experimental and control group settings Experimental group: set up one experimental group for each mixing ratio to ensure that the mixing ratio of photosensitive material and culture medium in each group is consistent; Control group: A control group without photosensitive materials was set up to compare its metabolic activity and light response ability; Experiments have shown that the photosensitive enzyme exhibits a significant increase in metabolic activity in the concentration range of 0.001%-0.1% (w / v), especially at a mixing ratio of 1:50, where the acid content changes significantly and the gas production remains stable, making it suitable as the best photosensitive material. When the concentration of photosensitive polymer is 1%-5%, it shows a weak effect, the acid amount increases relatively steadily, and the gas output does not change significantly. Its concentration and mixing ratio need to be further optimized; Although photosensitive algae can cause an increase in acid at a concentration of 0.1%-1%, the effect is relatively weak and may be suitable for conditions with lower light intensity or long-term exposure. According to the above experimental results, the experimental group with a mixing ratio of photosensitive enzyme to culture medium of 1:50 showed the best effect, which served as the basis for developing an organic fertilizer deodorizing fermentation agent.

[0031] S4: Lighting Condition Settings Light source selection: According to the response characteristics of the photosensitive material, select the appropriate light source, set the irradiation intensity and irradiation time of the light source, and adjust it according to the response of different experimental groups; By comparing the effects of different light sources (ultraviolet light, visible light and blue light) on the fermentation of photosensitizer and culture medium at a mixing ratio of 1:50, the choice of light source and light intensity was optimized; Detailed steps of the illumination experiment: S10: Prepare experimental materials According to the optimal mixing ratio (1:50) obtained from the mixing ratio experiment, prepare the photosensitive enzyme solution, prepare an appropriate amount of microbial culture medium, add appropriate concentrations of carbon source (1%-3%) and nitrogen source (0.1%-1%), and ensure that the culture medium meets the growth requirements of the microorganisms selected for the experiment.

[0032] Mix the photosensitive enzyme solution and culture medium in a mass ratio of 1:50. Ensure that the mixture is evenly mixed. Use a magnetic stirrer or oscillate to mix.

[0033] S20: Light source preparation Choose different light sources (ultraviolet light, visible light, blue light, etc.) to conduct experiments, each light source corresponds to a different light intensity; UV light: light intensity was set at 10 μmol / m² / s; Visible light: light intensity was set at 50 μmol / m² / s; Blue light: Light intensity was set at 20 μmol / m² / s.

[0034] Adjust the light intensity according to the light source type to ensure that the lighting conditions of the experimental group meet the preset intensity; S30: Culture medium inoculation Inoculate the prepared photosensitive enzyme and culture medium mixture into the culture dishes or culture bottles of the experimental group and the control group respectively. Each group uses the same volume of culture medium and mixed solution.

[0035] Label each experimental group and ensure that the mixture is evenly distributed within each group.

[0036] S40: Lighting Condition Settings Experimental groups: Each group of cultures was placed in a different light environment, according to the experimental design: Experimental group 1 (UV light, 10 μmol / m² / s): Place the culture dish under the UV light source and adjust the irradiation intensity.

[0037] Experimental group 2 (visible light, 50 μmol / m² / s): Place the culture dish under a visible light source and adjust the irradiation intensity.

[0038] Experimental group 3 (blue light, 20 μmol / m² / s): Place the culture dish under the blue light source and adjust the irradiation intensity.

[0039] Control group: Set up no-light conditions and place the control group cultures in a no-light environment as a basal metabolic control.

[0040] S50: Metabolite Detection After the set illumination time, samples were collected and each group of samples was tested for metabolites. The illumination experiment showed that ultraviolet light showed the most significant effect, especially the increase in acid amount, indicating that ultraviolet light can effectively activate photosensitive enzymes, thereby promoting the degradation reaction of photosensitive enzymes, and can help remove harmful odors by enhancing the production of acidic metabolites.

[0041] The effect of visible light is relatively balanced, with both acid and gas production increasing to a certain extent, indicating that visible light promotes fermentation. The increase in gas production means that visible light may promote the release of certain volatile gases during metabolism, which is helpful for the ventilation and deodorization of organic fertilizers. The effect of blue light was relatively mild, and the changes in acid and gas production were not obvious, indicating that blue light may have little effect on the metabolic activity of microorganisms. Although blue light can regulate microbial growth under certain conditions, it is not the best choice in the fermentation process of photosensitive enzymes and culture media. The metabolites in the no-light group changed less, indicating that the lack of light can lead to incomplete or slowed metabolic reactions of microorganisms.

[0042] Step 3: Microbial inoculation and cultivation Inoculation preparation: Inoculate microorganisms such as complex Bacillus, lactic acid bacteria, yeast, nitrifying bacteria, actinomycetes, anaerobic bacteria, and phosphate-solubilizing bacteria. Prepare a suitable liquid culture medium according to the growth requirements of the microorganisms. Sufficient carbon sources (such as glucose and starch), nitrogen sources (such as amino acids and urea) and other trace elements need to be added to the culture medium to ensure the growth and metabolism of various microorganisms. Mixed strain inoculation: Inoculate various microbial communities into the culture medium according to the preset strain ratio and ensure uniform distribution. The inoculation amount of different microbial communities can be optimized according to their respective bacterial characteristics (such as proliferation rate, metabolites).

[0043] According to the mixing ratio of the photosensitive enzyme and the culture medium and the ultraviolet light intensity determined in step 2, maintain appropriate ultraviolet irradiation conditions, use an ultraviolet light source for irradiation, ensure the activation of the photosensitive enzyme, and then promote the changes in the metabolic pathways of the microorganisms, cultivate the microorganisms under specific light conditions, and monitor the growth, reproduction, and metabolite production of the microorganisms; According to the growth curve of the microorganisms, culture for 24-48 hours, monitor the activity of the bacterial flora, temperature, pH value, etc. during the fermentation process to ensure the normal growth and metabolism of the microorganisms.

[0044] Step 4: Fermentation process control and optimization Set the temperature and humidity environment of the fermentation tank to ensure that the temperature is within the appropriate range to promote the activity of microorganisms and fermentation efficiency; A stirring device is used to ensure uniform distribution of microorganisms and avoid bacterial sedimentation. The stirring speed is adjusted according to the type of microorganisms and the viscosity of the fermentation matrix.

[0045] Aeration equipment should be installed to provide sufficient oxygen supply to promote the metabolic activities of aerobic microorganisms. For anaerobic bacteria, the oxygen concentration should be controlled to create a suitable anaerobic environment. During the fermentation process, the pH value is regularly tested to ensure that it is maintained within a range suitable for microbial growth; Take samples regularly during the fermentation process to detect changes in metabolites (such as gas production, acids, ammonia, etc.) and the growth of microbial populations; Compare the test results at different time points, evaluate the progress of the fermentation process, and determine whether adjustments are needed.

[0046] Step 5: Product harvesting and drying When the metabolic activity of the microorganisms in the fermentation broth tends to be stable and the metabolites reach the ideal level, the fermentation process can be ended. At this time, the microbial population in the fermentation tank and the substances in the fermentation broth should be fully mixed to achieve the effect of deodorizing the organic fertilizer. The microbial culture medium is separated from the generated metabolites by centrifugation, filtration or sedimentation to obtain the clear liquid or solid part. For organic fertilizer fermentation agents, the fermentation broth and the generated organic matter are usually dried. The fermentation broth is dried using drying equipment (such as a spray dryer, freeze dryer, etc.) to obtain a powdered or granular organic fertilizer deodorizing fermentation agent.

[0047] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. An organic material deodorizing fermentation agent, characterized in that: The invention comprises microbial raw materials, light-responsive materials and culture medium components. The microbial raw materials comprise 0.5% to 2% by weight of a complex bacillus group, 0.5% to 2% of a lactic acid bacteria group, 0.5% to 1% of a yeast group, 0.5% to 2% of a nitrifying bacteria group, 0.5% to 1.5% of an actinomycete group, 0.5% to 1% of an anaerobic bacteria group and 0.5% to 1% of a phosphate-solubilizing bacteria; the light-responsive materials comprise 0.001%-0.1% by weight of a photosensitizer, 1% to 5% of a photosensitive polymer and 0.1% to 1% of a photosensitive algae; the culture medium components comprise a carbon source in a concentration range of 1% to 3% (w / v) and a nitrogen source in a concentration range of 0.1% to 1% (w / v).

2. A method for preparing an organic material deodorizing fermentation agent, characterized in that: The method comprises the following preparation steps: Step 1: Preparation; Step 2: Microbial engineering and preparation of light-responsive materials; Step 3: Microbial inoculation and cultivation; Step 4: Fermentation process control and optimization; Step 5: Product harvesting and drying. Select microbial raw materials with strong metabolic activity and the ability to degrade odor, enhance their responsiveness to light activation through genetic engineering, prepare photosensitive enzymes, photosensitive polymers and photosensitive algae as photosensitive materials, mix the photosensitive materials with the microbial culture medium, and change the metabolic pathway of the microorganisms by adjusting the light-responsive activity.

3. The method for preparing an organic material deodorizing fermentation agent according to claim 2, characterized in that: In the second step, a microbial community with strong metabolic activity and capable of degrading odor is selected, including 0.5% to 2% of a complex bacillus community, 0.5% to 2% of a lactic acid bacteria community, 0.5% to 1% of a yeast community, 0.5% to 2% of a nitrifying bacteria community, 0.5% to 1.5% of an actinomycete community, 0.5% to 1% of an anaerobic bacteria community, and 0.5% to 1% of a phosphate-solubilizing bacteria mixture, and the responsiveness to light activation is enhanced by genetic engineering, and a photosensitive material is prepared, including a photosensitive enzyme with a concentration of 0.001% to 0.1%, a photosensitive polymer with a concentration of 1% to 5%, and a photosensitive algae with a concentration of 0.1% to 1%, and a microbial culture medium is prepared, including a carbon source with a concentration range of 1% to 3% (w / v) and a nitrogen source with a concentration range of 0.1% to 1% (w / v); By combining photosensitive materials with microbial flora, their light response activity can be adjusted under the irradiation of specific light sources, thereby changing the metabolic pathway of the microorganisms.

4. The method for preparing an organic material deodorizing fermentation agent according to claim 2, characterized in that: In the step 2, the mixing ratio of the photosensitive material and the microbial culture medium is obtained through a mixing experiment to obtain the optimal mixing ratio, and the mixing experiment steps include: S1: Mix the photosensitive material with the culture medium, prepare the photosensitive enzyme, photosensitive polymer and photosensitive algae solution according to the predetermined concentration, adjust the ratio to ensure that each photosensitive material is fully dissolved or evenly dispersed, prepare the culture medium and add appropriate concentrations of carbon source (1%-3%) and nitrogen source (0.1%-1%) according to the needs of the selected microorganisms, and adjust the culture medium composition according to the characteristics of different strains to meet their nutritional needs; S2: Mix the photosensitizer and the culture medium, set the ratio, mix according to different mass ratios of the photosensitizer and the culture medium, select several different ratios for experiments, 1:10 (photosensitive material: culture medium), 1:50 (photosensitive material: culture medium), 1:100 (photosensitive material: culture medium), and fully mix the photosensitizer solution and the culture medium.

5. The method for preparing an organic material deodorizing fermentation agent according to claim 2, characterized in that: The step 2, the mixing experiment step also includes: S3: Experimental group and control group were set up. One experimental group was set up for each mixing ratio to ensure that the mixing ratio of the photosensitive material and the culture medium in each group was consistent. A control group without photosensitive material was set up. The experimental results showed that the photosensitive enzyme showed a significant increase in metabolic activity, significant changes in acid content and stable gas production within the concentration range of 0.001%-0.1% (w / v) at a mixing ratio of 1:50, making it the best photosensitive material; S4: Setting of lighting conditions: According to the response characteristics of the photosensitive material, select the appropriate light source, set the irradiation intensity and irradiation time of the light source, and adjust them according to the reactions of different experimental groups. By comparing the fermentation effects of different light sources (ultraviolet light, visible light and blue light) on the mixture ratio of photosensitive enzyme and culture medium of 1:50, the selection of light source and light intensity can be optimized.

6. The method for preparing an organic material deodorizing fermentation agent according to claim 2, characterized in that: The second step includes a lighting experiment. The lighting condition setting is determined by the lighting experiment. The lighting experiment steps include: S10: Prepare experimental materials; S20: Light source preparation; S30: medium inoculation; S40: Lighting condition setting; S50: Metabolite detection.

7. The method for preparing an organic material deodorizing fermentation agent according to claim 6, characterized in that: In the illumination experiment, according to the optimal mixing ratio of 1:50, a photosensitive enzyme solution and a microbial culture medium are prepared, and appropriate concentrations of carbon source (1%-3%) and nitrogen source (0.1%-1%) are added to ensure that the culture medium meets the growth requirements of the microorganisms. The photosensitive enzyme solution and the culture medium are mixed at a mass ratio of 1:

50. Three light sources are selected in the experiment, including ultraviolet light, visible light and blue light, and the light intensities are set to 10 μmol / m² / s, 50 μmol / m² / s and 20 μmol / m² / s, respectively.

8. The method for preparing an organic material deodorizing fermentation agent according to claim 7, characterized in that: In the illumination experiment, the experimental group placed the culture dish or culture bottle in different illumination environments, with ultraviolet light, visible light and blue light corresponding to different illumination intensities, and the control group was placed in a no-light environment as a basal metabolic control to ensure the comparison of illumination effects, metabolite detection and result analysis The experimental results show that ultraviolet light has a significant effect on the increase in acid content, can effectively activate photosensitive enzymes and promote degradation reactions, and enhance the removal of harmful odors. Although visible light has a more balanced effect, with both acid and gas production increasing, blue light has little effect on metabolic reactions and does not significantly promote changes in acid and gas production. The no-light group showed a slowed or incomplete metabolic reaction, indicating the importance of light in this experiment.

9. The method for preparing an organic material deodorizing fermentation agent according to claim 2, characterized in that: The step three is to prepare a liquid culture medium suitable for the growth of microorganisms, and add carbon sources, nitrogen sources and trace elements to support the growth of microorganisms such as complex Bacillus groups and lactic acid bacteria groups, and evenly inoculate the microorganisms into the culture medium according to a preset strain ratio. According to the mixing ratio of the photosensitizer and the culture medium determined in step two, the photosensitizer is activated under ultraviolet irradiation conditions to promote the metabolic changes of the microorganisms.

10. The method for preparing an organic material deodorizing fermentation agent according to claim 2, characterized in that: In the fourth step, the oxygen supply is adjusted according to the type of microorganisms to ensure the growth requirements of aerobic and anaerobic bacteria, the pH value and metabolites in the fermentation liquid are regularly tested, and the fermentation process is optimized according to the test results.

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