Novel fluorescent dyes and polymers from dihydrophenanthrene derivatives

Abstract

The present disclosure provides novel dihydrophenanthrene (DHP)-cyanine and DHP-squarylium cyanine fluorescent compounds, and water soluble polymers thereof. DHP-cyanine and DHP-squarylium cyanine fluorescent compounds and polymers can be excited using UV, purple, blue, yellow, green, red, or NIR wavelengths. The fluorescent dye can be conjugated to an antibody for detecting a target analyte in a biological sample, and is suitable for flow cytometry analysis.

Description

[0001] This application was filed as a PCT international patent application on June 30, 2023, and claims the benefit and priority of U.S. Provisional Patent Application Serial No. 63 / 357,980 filed on July 1, 2022 and U.S. Provisional Patent Application Serial No. 63 / 490,921 filed on March 17, 2023. The entire disclosure of the above-mentioned U.S. Provisional Patent Application is incorporated herein by reference in its entirety. Background Art

[0002] There is an increasing demand for a wide range of fluorescent dyes for use in current flow cytometers and spectroscopic flow instruments. Water-soluble fluorescent compounds and their conjugates can be used in a variety of biological applications by generating signals that can be monitored in real time and provide a simple and rapid method for detecting biological targets and events, for example in diagnostic kits, in microscopy, in cytometry or in drug screening.

[0003] Molecular recognition involves the specific binding of two molecules. Molecules with binding specificity for a target biomolecule can be used in a variety of research and diagnostic applications, such as labeling and separation of analytes, flow cytometry, in situ hybridization, enzyme-linked immunosorbent assay (ELISA), Western blot analysis, magnetic cell separation, and chromatography. Target biomolecules can be detected by labeling with fluorescent dyes.

[0004] Current polymer dyes based on dihydrophenanthrene have strong excitation in the ultraviolet and violet regions between 350 and 450 nm. U.S. Patent No. 11,208,527 describes fluorescent polymer dyes based on water-soluble dihydrophenanthrene (DHP), for example, which exhibit an excitation maximum between 395 and 415 nm, with emission between about 415 and 475 nm. U.S. Patent No. 11,584,825 describes violet excitable polymers and tandem dyes based on water-soluble DHP.

[0005] The demand for multicolor panels for both conventional and spectral flow cytometry has increased, which require additional fluorescent dyes that can be excited with other lasers (e.g., 488nm, 563nm, 638nm, and 808nm). Parameters that users consider when selecting fluorescent dyes may include the excitation wavelength maximum, the emission wavelength maximum, the brightness of the dye, and the fluorescence lifetime. The brightness of a dye is the overall contribution from the extinction coefficient (ε, a measure of the amount of light absorbed at a specific wavelength) and the fluorescence quantum yield (Φ, a measure of the light emitted as radiation from its singlet excited state).

[0006] Attempts to further move the excitation range to the red region by incorporating modifier unit monomers into existing DHP purple excitable polymer backbones result in retention of absorption from acceptor dyes and the original absorption of polymers. Direct excitation of the resulting DHP modifier unit polymer dye results in strong UV and violet emission from the core. Attempts to use other aromatic cyclic molecules as monomers to design red shifted core polymers have encountered synthetic challenges. Therefore, more careful design is needed to move the excitation maximum to the blue, green and red regions of the spectrum and further regions.

[0007] Organic fluorescent dyes and water-soluble polymer dyes that can be excited using UV, violet, blue, yellow, green, red, and near infrared (NIR) wavelengths are desirable. Summary of the invention

[0008] The present disclosure generally provides dihydrophenanthrene (DHP)-cyanine (Cy) and DHP-squaraine compounds and polymer dyes, water-soluble DHP-cyanine and DHP-squaraine compounds and water-soluble fluorescent polymers conjugated to specific binding partners, their complexes, and methods of using complexes comprising water-soluble DHP-Cy and DHP-squaraine fluorescent compounds or polymers conjugated to binding partners for detecting analytes in samples. Tandem dyes comprising DHP-Cy and DHP-squaraine fluorescent compounds, polymers or labeled specific binding partners according to the present disclosure are also provided. DHP-Cy and DHP-squaraine compounds, polymers, labeled specific binding partners and tandem dyes according to the present disclosure are used in biological applications, including for detecting target analytes and for diagnostic kits, etc. The kit may comprise a DHP-Cy compound, a DHP-squaraine compound, a polymer, a labeled specific binding partner and / or a tandem dye according to the present disclosure, optionally with a conjugated label.

[0009] The present disclosure provides fluorescent compounds comprising a structure according to formula (I): in: D is aryl, heteroaryl, J is aryl, heteroaryl or and at least one of D or J is independently selected from wherein the optional Ar and the optional are each independently selected from substituted or unsubstituted benzene, benzene derivatives, monocyclic aromatic groups, polycyclic aromatic groups, monocyclic heteroaromatic groups and polycyclic heteroaromatic groups, wherein at least one of D or J is present and contains or a derivative thereof, said derivative comprising an additional aryl or heteroaryl fused at any available position, wherein: each T is independently selected from C, C(R 1 ), N, P, O, S, and Si(R 1 ); each U is independently selected from NR 10 , O, P, and S; each V is independently selected from NR 11 , CR 11 , C(R 11 )2, S, SO2, O, and Si(R 11 )2; each V’ is independently SO2, SO, S, NR 11 , CR 11 , C(R 11 )2, O, Si(R 11 )2, >C=O, >Se=O, -CH=CH-, or -N=CH-; each X is independently CR 1 R 2 or SiR 1 R 2 ; each Y is independently CR 8 R 9 or SiR 8 R 9 ; wherein each R 1 , R 2 , R 8 , and R 9 is independently selected from a water-soluble moiety, a linker moiety, a linked E, hydrogen, hydroxy, halogen, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, haloalkyl, aryl, heteroaryl, (hetero)aryloxy, (hetero)arylamino, sulfonamide-PEG, phosphonamide-PEG, alkylammonium salt, alkyloxyammonium salt, carbonyl, acyl, oligomeric ether ammonium salt, alkylsulfonate, alkoxysulfonate, oligomeric ether sulfonate, sulfonamido oligomeric ether, sulfonamide, sulfinamide, phosphonamidate, phosphinate, alkoxysulfonamide PEG, alkoxysulfonate, alkylsulfonate, alkylsulfonate, optionally, wherein R 1 and R 8 together form an unsubstituted or substituted cycloalkyl, cycloalkenyl, heterocycloalkyl, heterocycloalkenyl, cycloalkoxy, aryl, or heteroaryl having 3 to 9 ring members; each R 3independently selected from H, alkyl, alkene, alkyne, cycloalkyl, haloalkyl, alkoxy, (hetero)aryloxy, aryl, (hetero)arylamino, a water soluble moiety, a chromophore, a functional moiety, a binding partner, and a PEG group; Each Q is independently a bond, NH, NR 4 、C1-C 12 Alkylene, CHR 4 and CH2 CH2, CHR 4 , O, NR 4 or NH; Each Z is independently CH2, CHR 4 , O, NR 4 or NH; Each W 1 independently a water-soluble portion; L 1 , L 2 and L 3 are each independently selected linker moieties; Each E is independently selected from a chromophore, a functional moiety, a substrate, and a binding partner; Each R 4 independently selected from H, alkyl, PEG, a water soluble moiety, a linker moiety, a chromophore, a functional group, a conjugated tag, a carboxylic acid amide, an amine, a carbamate, a carboxylic acid, a carboxylate, a maleimide, an activated ester, an N-hydroxysuccinimidyl group, a hydrazine, a hydrazone, an azide, an alkyne, an aldehyde, and a thiol, or a protected group thereof, Each R 7 Independently selected from H, hydroxyl, C1-C 12 Alkyl, C2-C 12 Olefins, C2-C 12 Alkynes, C3-C 12 Cycloalkyl, C1-C 12 Halogenated alkyl, C1-C 12 Alkoxy, C2-C 18 (Hetero)aryloxy, C2-C 18 (Hetero)arylamino, C2-C 12 Carboxylic acid, C2-C 12 Carboxylic acid esters and C1-C 12 Alkoxy; Each R 10 independently selected from hydrogen, a linker moiety, an attached reactive group, an attached ionic group, an attached chromophore, an attached water-soluble moiety, a water-soluble moiety, a chromophore, a binding partner, a attached binding partner; Each R 11Independently selected from hydrogen, a linker moiety, a linked reactive group, a linked ionic group, a linked chromophore, a linked water-soluble moiety, a water-soluble moiety, a chromophore, a binding partner, and a linked binding partner; Each R 12 and R 13 are independently selected from hydrogen, C1-C6 alkyl, C1-C6 alkyl, C3-C 10 cycloalkyl, substituted or unsubstituted aryl, and substituted or unsubstituted heteroaryl; or R 12 and R 13 together form an unsubstituted or substituted unsaturated cycloalkyl having 3 to 8 ring members, or a substituted or unsubstituted heterocycloalkyl having 3 to 8 ring members, which is optionally substituted with O; Each K is independently a covalent bond, O, S, P, or CH2 Each f is independently an integer from 0 to 50. Each m is independently 0 or 1; Each n is independently 0, 1, 2, 3, or 4 Each s is independently 1 or 2; and Each t is independently 0, 1, 2, or 3.

[0010] The present disclosure provides a fluorescent compound comprising a structure according to formula (II): Wherein: Each is independently selected from substituted or unsubstituted benzene, benzene derivatives, monocyclic aryl, polycyclic aryl, monocyclic heteroaryl, and polycyclic heteroaryl, wherein at least one comprises or a derivative thereof, wherein the derivative optionally comprises an additional aryl or heteroaryl fused at any available position, wherein: Each V’ is independently SO2, SO, S, NR 11 , CR 11 , C(R 11 )2, O, Si(R 11 )2, >C=O, >Se=O, -CH=CH-, or -N=CH-; Each T is independently selected from C, C(R 1 ), N, P, O, S, and Si(R 1 ); Each U is independently selected from NR 10 , O, P, Se, Te, and S; Each V is independently selected from NR 11 , CR 11, C(R 11 )2, S, SO2, O, Se, Te and Si(R 11 )2; Each X is independently CR 1 R 2 or SiR 1 R 2 ; Each Y is independently CR 8 R 9 or SiR 8 R 9 ; in: Each R 1 , R 2 , R 8 and R 9 independently selected from a water soluble moiety, a linked water soluble moiety, a linker moiety, a linked E, a reactive group, a linked reactive group, a binding partner, a linked binding partner, a functional group, hydrogen, hydroxyl, halogen, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, haloalkyl, aryl, heteroaryl, (hetero)aryloxy, (hetero)arylamino, sulfonamide-PEG, phosphoamide-PEG, alkylammonium salt, alkyloxyammonium salt, oligoether ammonium salt, carbonyl, acyl, alkylsulfonate, alkoxysulfonate, oligoethersulfonate, sulfonamidooligoether, sulfonamide, sulfenamide, phosphoamidate, phosphinamide, alkoxysulfonamide PEG, alkoxysulfonate, alkylsulfonate, alkylsulfonate, alkylsulfonate, Optionally, wherein R 1 and R 8 together form an unsubstituted or substituted cycloalkyl, cycloalkenyl, heterocycloalkyl, heterocycloalkenyl, cycloalkoxy, aryl or heteroaryl group having 3 to 9 ring members; Each R 3 independently selected from H, alkyl, alkene, alkyne, cycloalkyl, haloalkyl, alkoxy, (hetero)aryloxy, aryl, (hetero)arylamino, a water soluble moiety, a chromophore, a functional moiety, a binding partner, and a PEG group; Each Q is independently a bond, NH, NR 4 、C1-C 12 Alkylene, CHR 4 or CH2; Each Z is independently CH2, CHR 4 , O, NR 4 or NH; Each W 1 independently a water-soluble portion; L 1 , L 2 and L3 are each independently selected linker moieties; Each E is independently selected from a chromophore, a functional moiety, a substrate, and a binding partner; Each R 4 independently selected from H, alkyl, PEG, a water soluble moiety, a linker moiety, a chromophore, a functional group, a carboxylic acid amide, an amine, a carbamate, a carboxylic acid, a carboxylic acid ester, a maleimide, an activated ester, an N-hydroxysuccinimidyl group, a hydrazine, a hydrazone, an azide, an alkyne, an alkene, a tetrazine, an aldehyde, and a thiol, or a protected group thereof, Each R 7 Independently selected from H, hydroxyl, C1-C 12 Alkyl, C2-C 12 Olefins, C2-C 12 Alkynes, C3-C 12 Cycloalkyl, C1-C 12 Halogenated alkyl, C1-C 12 Alkoxy, C2-C 18 (Hetero)aryloxy, C2-C 18 (Hetero)arylamino, C2-C 12 Carboxylic acid, C2-C 12 Carboxylic acid esters and C1-C 12 Alkoxy; Each R 10 independently selected from hydrogen, a linker moiety, an attached reactive group, an attached ionic group, an attached chromophore, an attached water-soluble moiety, a water-soluble moiety, a chromophore, a binding partner, a attached binding partner; Each R 11 independently selected from hydrogen, a linker moiety, an attached reactive group, an attached ionic group, an attached chromophore, an attached water-soluble moiety, a chromophore, a binding partner, and an attached binding partner; Each R 12 and R 13 independently selected from hydrogen, halogen, substituted or unsubstituted C1-C6 alkyl, substituted or unsubstituted C1-C6 heteroalkyl, substituted or unsubstituted C1-C6 alkene, substituted or unsubstituted C3-C 10 cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, and substituted or unsubstituted heteroaryl; or R 12 and R 13 together form an unsubstituted or substituted unsaturated cycloalkyl having 3 to 8 ring members, or a substituted or unsubstituted heterocycloalkyl having 3 to 8 ring members, which is optionally substituted with O; Each K is independently a covalent bond, O, S, P, NR 1 , Se, Te, CR1 R 2 or CH2; Each f is independently an integer from 0 to 50; Each m is independently 0 or 1; Each n is independently 0, 1, 2, 3 or 4; each s is independently 1 or 2; and Each t is independently 0, 1, 2 or 3.

[0011] Included in any available Another aryl or heteroaryl group fused to The derivative may optionally contain Where V' is SO2, SO, S, NR 11 , CR 11 , C(R 11 )2、O、Si(R 11 )2, >C=O, >Se=O, -CH=CH- or -N=CH-, and T, V, X, Y and m are as defined above.

[0012] The present disclosure provides DHP-cyanine and DHP-squarylium cyanine compounds selected from Formula (IIa), (IIb), (IIc), (IId), (IIe), (IIf), (IIg), (IIh), (IIi), (IIj), (Ik), (IIl), (IIm), (IIn), (IIo), (IIp), (IIq), (IIr), (IIs), (IIt), (IIu), (IIv), (IIw), (IIx), (IIy), and (IIz):

[0013] The present disclosure provides DHP-cyanine and DHP-squarylium cyanine compounds selected from Formula (IIIa), (IIIb), (IIIc), (IIId), (IIIe), (IIIf), (IIIg), (IIIh), (IIIi), (IIIj), (IIIk), (IIIl), (IIIm), (IIIn), (IIIo), (IIIp), (IIIq), (IIIr), (IIIs), (IIIt), (IIIu), (IIIv), (IIIw), (IIIx), (IIIy), (IIIz), (IIIaa), (IIIbb), (IIIcc), (IIIdd), and (IIIee):

[0014] In some embodiments, D and J are The group contains the same or different group or its derivatives.

[0015] In some embodiments, D and J are Groups contain the same group or its derivatives.

[0016] In some embodiments, D and J The group contains different group or its derivatives.

[0017] In some embodiments, D is D yes or its derivatives; and J is aryl, heteroaryl or Among them, J It is an unsubstituted or substituted benzene, a benzene derivative containing a fused monocyclic aromatic group, a polycyclic aromatic group, a monocyclic heteroaromatic group or a polycyclic heteroaromatic group.

[0018] In some embodiments, J is Among them, J is unsubstituted or substituted benzene or unsubstituted or substituted naphthalene; U is N; and V is N, O or S.

[0019] In some embodiments, J is unsubstituted or substituted quinoline, benzo Azoles, benzothiazoles, benzimidazoles, indoles, benzindole, pyridine Benzopyran Thiopyran Fluorene, 9H-fluorene, phenanthrene, dihydrophenanthrene, 9,10-dihydrophenanthrene, 6,8-dihydro-5H-naphtho[2,1-f]indole, 4,5-dihydro-3H-naphtho[2,1-e]indole, 6,7-dihydro-3H-naphtho[2,1-g]indole, 5,6-dihydrophenanthrene [3,2-d]thiazole, 4,5-dihydrophenanthro[2,1-d]thiazole, 6,7-dihydrophenanthro[4,3-d]thiazole, 5,6-dihydrophenanthro[3,2-d]thiazole oxadiazole, 4,5-dihydrophenanthro[2,1-d] oxadiazole, 6,7-dihydrophenanthro[4,3-d] oxadiazole, 5,6-dihydronaphtho[2,1-g]quinoline, 7,8-dihydronaphtho[2,1-h]quinoline, 5,6-dihydronaphtho[2,1-f]quinoline, 5,6-dihydro-8λ2-phenanthro[3,2-d]imidazole, 4,5-dihydro-3λ2-phenanthro[2,1-d]imidazole, 6,7-dihydro-3λ2-phenanthro[4,3-d]imidazole, 5,6-dihydronaphtho[1,2-g]quinoxaline, 5,6-dihydronaphtho[2,1-f]quinoxaline, 7,8-dihydronaphtho[1,2-f]quinoxaline, phenyl, 1H-benzo[e]indole-3- 1H-Benzo[e]indole and 9,10-dihydro-8H-thieno[2',3',4',5':4,5]phenanthro[2,1-b]pyrrole 4,4-dioxide.

[0020] In some embodiments, D is aryl, heteroaryl or Among them, D is unsubstituted or substituted benzene, a benzene derivative, a monocyclic aryl group, a polycyclic aryl group, a monocyclic heteroaryl group or a polycyclic heteroaryl group; and J is Among them, J yes or its derivatives.

[0021] In some embodiments, D is Among them, D is unsubstituted or substituted benzene or unsubstituted or substituted naphthalene; U is N; and V is N, O or S.

[0022] In some embodiments, D is selected from unsubstituted or substituted quinoline, benzo Azoles, benzothiazoles, benzimidazoles, indoles, benzindole, pyridine Benzopyran Thiopyran Fluorene, 9H-fluorene, phenanthrene, dihydrophenanthrene, 9,10-dihydrophenanthrene, 6,8-dihydro-5H-naphtho[2,1-f]indole, 4,5-dihydro-3H-naphtho[2,1-e]indole, 6,7-dihydro-3H-naphtho[2,1-g]indole, 5,6-dihydrophenanthrene [3,2-d]thiazole, 4,5-dihydrophenanthro[2,1-d]thiazole, 6,7-dihydrophenanthro[4,3-d]thiazole, 5,6-dihydrophenanthro[3,2-d]thiazole oxadiazole, 4,5-dihydrophenanthro[2,1-d] oxadiazole, 6,7-dihydrophenanthro[4,3-d] oxadiazole, 5,6-dihydronaphtho[2,1-g]quinoline, 7,8-dihydronaphtho[2,1-h]quinoline, 5,6-dihydronaphtho[2,1-f]quinoline, 5,6-dihydro-8λ2-phenanthro[3,2-d]imidazole, 4,5-dihydro-3λ2-phenanthro[2,1-d]imidazole, 6,7-dihydro-3λ2-phenanthro[4,3-d]imidazole, 5,6-dihydronaphtho[1,2-g]quinoxaline, 5,6-dihydronaphtho[2,1-f]quinoxaline, 7,8-dihydronaphtho[1,2-f]quinoxaline, phenyl, 3,5,6,8-tetrahydrophenanthro[2,3-e:7,6-e']diindole, 5,6-dihydropentaphenanthro[3,4-d:10,9-d']bis( azole), 5,6-dihydropentaphenanthro[3,4-d:10,9-d']bis(thiazole), 6,7-dihydrophenanthro[2,3-f:7,6-f']biquinoline, 5,6-dihydropentaphenanthro[3,4-d:10,9-d']bis(thiazole), [3,2,1-cd:10,11,12-c'd']diindole, 2,6,7,11-tetrahydrobenzo[1,2-g:4,3-g']dichromene, 2,6,7,11-tetrahydrobenzo[1,2-g:4,3-g']bis(thiochromene) and 9,10-dihydro-8H-thieno[3,2,1-cd:10,11,12-c'd']diindole, 2,6,7,11-tetrahydrobenzo[1,2-g:4,3-g']dichromene [2',3',4',5':4,5]phenanthro[2,1-b]pyrrole 4,4-dioxide.

[0023] In some embodiments, D is Or J is At least one of yes A group or a derivative thereof, wherein the derivative Any available Location and Condensed. The derivatives may be included in any available The additional aryl or heteroaryl group fused to Where V' is SO2, SO, S, NR 11 , CR 11 , C(R 11 )2、O、Si(R 11 )2, >C=O, >Se=O, -CH=CH- or -N=CH-, and T, V, X, Y and m are as defined above.

[0024] In some embodiments, D is and The group or its derivatives Any available Location and Condensed.

[0025] In some embodiments, D is selected from:

[0026] In some embodiments, D is Among them, D yes A group or a derivative thereof, wherein the derivative Any available Location and Condensed.

[0027] In some embodiments, J is in The group is A group or a derivative thereof, wherein the derivative Any available Location and Condensed. The derivatives may be included in any available The additional aryl or heteroaryl group fused to Where V' is SO2, SO, S, NR 11 , CR 11 , C(R 11 )2、O、Si(R 11 )2, >C=O, >Se=O, -CH=CH- or -N=CH-, and T, V, X, Y and m are as defined above.

[0028] In some embodiments, J is selected from:

[0029] In some embodiments, at least one The group or its derivatives are selected from: Each R 5 independently selected from halogen, hydroxyl, C1-C 12 Alkyl, C2-C 12 Olefins, C2-C 12 Alkynes, C3-C 12 Cycloalkyl, C1-C 12 Halogenated alkyl, C1-C 12Alkoxy, C2-C 18 (Hetero)aryl, C2-C 18 (Hetero)aryloxy, C2-C 18 (Hetero)arylamino, carboxylic acid, carboxylate, (CH2) x’ (OCH2-CH2) y’ OCH3 and (CH2) x’ (OCH2-CH2) y’ OCF3, wherein each x' is independently an integer from 0 to 20, and each y' is independently an integer from 0 to 50.

[0030] In some embodiments, D is selected from:

[0031] In some embodiments, J is selected from:

[0032] In some embodiments, R 10 selected from the group consisting of a water soluble moiety, a linker moiety, a linked water soluble moiety, hydrogen, an alkyl group, an alkene group, an alkyne group, a cycloalkyl group, a haloalkyl group, a (hetero)aryloxy group, an aryl group, a heteroaryl group, a (hetero)arylamino group, a sulfonamide-PEG, a phosphoamide-PEG, an alkylammonium salt, an alkyloxyammonium salt, an oligoether ammonium salt, an alkylsulfonate, an alkoxysulfonate, an oligoethersulfonate, a sulfonamidooligoether, a sulfonamide, a sulfenamide, a phosphoamidoester, a phosphinamide, an alkoxysulfonamide PEG, an alkoxysulfonate, an alkylsulfonate, an alkylsulfonate,

[0033] In some cases, a fluorescent dye is provided that comprises a structure according to any of the following:

[0034] In some cases, an acceptor dye is provided that includes a DHP-cyanine compound having a structure according to formula (II).

[0035] The present disclosure provides polymeric dyes comprising a monomer having a structure according to formula (IV) or (V): Where each of (IV) or (V) is the point of attachment to the polymer dye backbone; each D 1 and D 2 are independently selected from aryl, heteroaryl and Each of these are independently selected from substituted or unsubstituted benzene, benzene derivatives, monocyclic aromatic groups, polycyclic aromatic groups, monocyclic heteroaromatic groups and polycyclic heteroaromatic groups, wherein D 1 or D 2 At least one of in yes or its derivatives, said derivatives being included in any available fused to another aryl or heteroaryl group, and wherein The group or its derivatives Any available Location and fused; and T, U, V, X, Y, R 1 , R 2 , R 3 , R 4 , R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , R 13 ,Q,Z,W1,L 1 , L 2 , L 3 Each of , E, k, f, n, m, n, s, t is as described herein.

[0036] The derivatives may be included in any available The additional aryl or heteroaryl group fused to Where V' is SO2, SO, S, NR 11 , CR 11 , C(R 11 )2、O、Si(R 11 )2, >C=O, >Se=O, -CH=CH- or -N=CH-, and T, V, X, Y and m are as defined above.

[0037] In some embodiments, the monomer of formula (IV) or (V) comprises a structure selected from formula (A1), (A2), (A3), (A4), (A5), (A6), (A7), (A8), (A9), (A10), (A11), (A12), (A13), (A14), (A15), (A16), (A17), (A18), and (A19):

[0038] The present disclosure provides polymeric dyes comprising a structure according to formula (VI) or (VII): Each D 1 and D 2 are independently selected from aryl, heteroaryl and Each of these are independently selected from substituted or unsubstituted benzene, benzene derivatives, monocyclic aromatic groups, polycyclic aromatic groups, monocyclic heteroaromatic groups and polycyclic heteroaromatic groups, wherein D 1 or D 2 At least one of in yes or its derivatives, said derivatives being included in any available fused to another aryl or heteroaryl group, and wherein The group or its derivatives Any available Location and condense; Each J is independently aryl, heteroaryl, in is substituted or unsubstituted benzene, benzene derivatives, monocyclic aromatic groups, polycyclic aromatic groups, monocyclic heteroaromatic groups, and polycyclic heteroaromatic groups; and p is an integer from 2 to 1,000; 5 to 500 or 10 to 100. Optionally, at least one J group is in The group is group or its derivatives, said derivatives being included in any available fused to another aryl or heteroaryl group, and wherein The group or its derivatives Any available Location and Condensed.

[0039] The present disclosure provides polymeric dyes having the structure of formula (VI'): in A is a monomer comprising a structure according to formula (IV) or (V) wherein each of formula (IV) or (V) is the point of attachment to the polymer dye backbone; each D 1 and D 2 are independently selected from aryl, heteroaryl and Each of these are independently selected from substituted or unsubstituted benzene, benzene derivatives, monocyclic aromatic groups, polycyclic aromatic groups, monocyclic heteroaromatic groups and polycyclic heteroaromatic groups, wherein D 1 or D 2 At least one of in yes or its derivatives, said derivatives being included in any available fused to another aryl or heteroaryl group, and wherein The group or its derivatives Any available Location and condense; Each optional M is a polymer modification unit uniformly or randomly distributed along the polymer backbone and is optionally substituted by one or more optionally substituted R 1 , R 2 , R 3 or R 4 group substitution; Each optional L is a linker; G 1 and G 2 are each independently selected from an unmodified polymer terminus and a modified polymer terminus, optionally conjugated to E; a, c, and d define the mol% of each unit within the structure, each of which may be repeated uniformly or randomly, and wherein each a is from 10% to 100% mol%, each c is from 0% to 90% mol%, and each d is from 0% to 25% mol%; Each b is independently 0 or 1; p is an integer from 1 to about 10,000; and T, U, V, X, Y, R 1 , R 2 , R 3 , R 4 , R 7 , R 8 , R9 , R 10 , R 11 , R 12 , R 13 ,Q,Z,W1,L 1 , L 2 , L 3 Each of , E, k, f, n, m, n, s, t is as described herein.

[0040] In some embodiments, G 1 and G 2 independently selected from hydrogen, halogen, alkyne, optionally substituted aryl, optionally substituted heteroaryl, aryl substituted by halogen, silyl, diazonium salt, triflate, acetoxy, azide, sulfonate, phosphate, boronic acid substituted aryl, boronic ester substituted aryl, borate, boronic acid, optionally substituted tetrahydropyrene (THP), optionally substituted fluorene, optionally substituted dihydrophenanthrene (DHP), aryl or heteroaryl, substituted with one or more side chains, the side chains being terminated with a functional moiety selected from amine, carbamate, carboxylic acid, carboxylate, maleimide, activated ester, N-hydroxysuccinimidyl, hydrazine, hydrazide, hydrazone, azide, alkyne, aldehyde, thiol and protected groups thereof, optionally conjugated to a substrate or binding partner.

[0041] In some embodiments, D 1 and D 2 In some embodiments, D 1 and D 2 are different.

[0042] In some embodiments, R 1 , R 2 , R 3 , R 4 , R 8 , R 9 , R 10 and R 11 At least one or at least both of the compounds comprise a water-soluble portion or a linked water-soluble portion.

[0043] The present disclosure provides a labeled specific binding partner comprising a fluorescent compound or polymer according to the present disclosure; and a specific binding partner covalently linked to the fluorescent compound or polymer. The specific binding partner can be selected from proteins, peptides, affinity ligands, antibodies, antibody fragments, carbohydrates, lipids, nucleic acids and aptamers. The specific binding partner can be an antibody. The specific binding partner can be an antibody specific for the target analyte.

[0044] The present disclosure provides tandem dyes comprising: a fluorescent compound, polymer or labeled specific binding partner according to the present disclosure; and an acceptor chromophore covalently linked to the fluorescent compound, polymer or labeled specific binding partner. In some cases, the fluorescent compound of the present invention is an acceptor dye.

[0045] The fluorescent compound, polymer, labeled specific binding partner or tandem dye may be water soluble.

[0046] A method for detecting a target analyte in a sample is provided, comprising: providing a sample suspected of containing the analyte; and contacting the sample with a specific binding partner conjugated to a water-soluble fluorescent compound, polymer or tandem dye according to the present invention, wherein the binding partner is capable of interacting with the target analyte.

[0047] The method may include one or more of the following: wherein the method is configured for flow cytometry; the water-soluble fluorescent polymer is bound to a substrate; the analyte is a protein expressed on the cell surface; the method is configured as an immunoassay; and / or the method further includes providing additional binding partners for simultaneously detecting additional analytes.

[0048] The present disclosure provides a kit comprising at least one of a fluorescent compound, a polymer, a labeled specific binding partner, or a tandem dye according to the present disclosure. The compound, polymer, or tandem dye according to the present disclosure may comprise a conjugated tag. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 The method for producing DHP-indole, DHP-thiazole, DHP- Synthesis scheme 1 of DHP-indole, DHP-thiazole, DHP- Oxazole and DHP-quinoline intermediates can be prepared from the bromo-DHP core molecule and converted to DHP-cyanine or DHP-squarylium compounds. The dotted circle represents the heterocyclic ring portion of the intermediate precursor.

[0050] Figure 2 Synthetic Scheme 2 for the modification of dihydrophenanthrene and subsequent synthesis of DHP-Cy5 type monomeric dyes is shown.

[0051] Figure 3 Synthesis Scheme 3 for the preparation of DHP-cyanine polymer dyes from a dihydrophenanthrene core is shown. DHP-cyanine and DHP-squarylium polymer dyes can exhibit excitation λmax in the range of 420 nm to 900 nm.

[0052] Figure 4Scheme 4 shows the synthesis of the modification of dihydrophenanthrene followed by the synthesis of polymeric DHP-Cy5 dye.

[0053] Figure 5 Shown are synthetic scheme 5 for preparing asymmetric DHP-squarylium monomer dyes from a dihydrophenanthrene-type core and semisquarylium; and synthetic scheme 6 for preparing symmetric DHP-squarylium monomer dyes from a dihydrophenanthrene-type core and squarylium.

[0054] Figure 6 The intermediate compound DHP-indole for preparing N-propyl sulfonate from dihydrophenanthrene is shown. 21 Synthesis Scheme 7.

[0055] Figure 7 The intermediate compound DHP-indole from -propyl sulfonate is shown 21 Preparation of Sulfoxide-bridged DHP Indocyanine Dyes 25 Synthetic Schemes 8 and 9.

[0056] Figure 8 Shown is a DHP indocyanine dye bridged by sulfoxide 25 Preparation of sulfoxide-bridged DHP indocyanine dye-antibody conjugates 27 Synthesis Scheme 10.

[0057] Fig. 9 Exemplary synthetic schemes 12 and 13 for the preparation and purification of DHP-cyanine-antibody conjugates or polymer-antibody conjugates are shown, respectively.

[0058] Fig.10 The intermediate compound DHP-indole from N-propyl sulfonate is shown 33 (which is made of chloroaminobenzoic acid 28 Preparation of sulfoxide-bridged DHP indole cyanine dyes 36 Exemplary synthetic scheme 14. DETAILED DESCRIPTION I. Overview

[0059] The present disclosure provides new DHP-cyanine and DHP-squaryl cyanine fluorescent compounds and polymers thereof. In some embodiments, the DHP-cyanine and DHP-squaryl cyanine fluorescent compounds and polymer dyes are designed to be water-soluble. The present disclosure also provides labeled specific binding partners comprising the DHP-cyanine and DHP-squaryl cyanine fluorescent compounds and polymers thereof. The present disclosure also provides tandem dyes comprising the DHP-cyanine and DHP-squaryl cyanine fluorescent compounds and polymers thereof. The DHP-cyanine and DHP-squaryl cyanine fluorescent compounds can be receptor dyes.

[0060] Methods for detecting target analytes in samples using fluorescent DHP-cyanine and DHP-squaraine compounds or polymers conjugated to binding partners are provided. Various DHP-cyanine and DHP-squaraine fluorescent compounds and polymers of the present disclosure exhibit water solubility and can be excited using UV, violet, blue, yellow, green, red or NIR wavelengths. There is an increasing demand for a variety of fluorescent dyes used in current flow cytometers and spectroscopic flow instruments.

[0061] In the present disclosure, dihydrophenanthrene monomers are modified to contain fused heterocyclic rings. For example, DHP monomers are modified to electron-rich indoles containing DHP-indole moieties using the Fischer indole synthesis, which produces aromatic heterocyclic indoles from substituted phenylhydrazines and aldehydes or ketones under acidic conditions. This can be performed in two steps using bromodihydrophenanthrene as a starting material, for example, as shown in Scheme 2. Classical cyanine dye synthesis is then performed on the DHP-indole ring to allow the formation of DHP-cyanine dyes, which can be excited using UV, violet, blue, green, red, or NIR light, depending on the number of carbon atoms in the linker molecule used for the cyanine synthesis.

[0062] A variety of chemical modifications of the core DHP-cyanine molecule allow the preparation of dyes that can be excited using different lasers. Both small organic dyes and polymeric dyes have been prepared using this method. Other families of dyes, such as squarylium, have also been prepared from electron-rich DHP ring cores. Symmetrical as well as asymmetric dyes are prepared using this method, which allows fine tuning of the absorption and emission of the dyes. For monomeric dyes, DHP-cyanine and DHP-squarylium dyes according to the present disclosure exhibit excitation maxima in the range of about 400 to 900 nm.

[0063] Also provided is a kit comprising at least one of a fluorescent DHP-cyanine compound, a DHP-squarylium compound or polymer thereof, a labeled specific binding partner, or a tandem dye according to the present disclosure. The fluorescent DHP-cyanine compound, the DHP-squarylium compound or polymer thereof, or the tandem dye may comprise a conjugated label. II. Definitions Abbreviations used herein have their conventional meanings in the fields of chemistry and biology. Reference will now be made in detail to certain embodiments of the disclosed subject matter, some examples of which are partially illustrated in the accompanying drawings.While the disclosed subject matter will be described in conjunction with the recited claims, it will be understood that the illustrated subject matter is not intended to limit the claims to the disclosed subject matter. Throughout this document, values ​​expressed in range format should be interpreted in a flexible manner to include not only the numerical values ​​explicitly recorded as limits of the range, but also all individual numerical values ​​or sub-ranges encompassed within the range, as if each numerical value and sub-range were explicitly recorded. For example, a range of "about 0.1% to about 5%" or "about 0.1% to 5%" should be interpreted to include not only about 0.1% to about 5%, but also individual values ​​(e.g., 1%, 2%, 3% and 4%) and sub-ranges (e.g., 0.1% to 0.5%, 1.1% to 2.2%, 3.3% to 4.4%) within the indicated range. Unless otherwise noted, the expression "about X to Y" has the same meaning as "about X to about Y". Similarly, unless otherwise noted, the expression "about X, Y or about Z" has the same meaning as "about X, about Y or about Z". In this document, unless the context clearly states otherwise, nouns modified by quantifiers are used to include one / kind or more / kind. Unless otherwise indicated, the term "or / or" is used to refer to a non-exclusive "or / or". The expression "at least one of A and B" or "at least one of A or B" has the same meaning as "A, B, or A and B". In addition, it should be understood that the words or terms used in this document (and not otherwise defined) are for description purposes only and not for limitation purposes. The use of any section headings is intended to help read the document and is not to be interpreted as limiting; information related to the section headings may appear in or outside the particular section. All publications, patents, and patent documents cited in this document are incorporated herein by reference as a whole, as if separately incorporated by reference. In the event of inconsistency between the usage of this document and those documents incorporated by reference, the usage in the incorporated references should be considered to be a supplement to the usage of this document; for irreconcilable inconsistencies, the usage in this document shall prevail. In the methods described herein, except when time or operation sequence is clearly listed, otherwise the action can be performed in any order without departing from the principle of the present disclosure. In addition, unless the clear claim language states that the specified action is performed separately, it can be performed simultaneously. For example, the action of performing X and the action of performing Y as claimed can be performed simultaneously in a single operation, and the resulting process will fall within the literal scope of the process as claimed. The term "about / approximately" used herein can allow a certain degree of variability of value or range, for example, within 10%, 5% or 1% of the value or the range limit, and includes the exact value or range. The term "substantially" used herein refers to most or most, such as at least about 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, 99.9%, 99.99%, or at least about 99.999% or greater, or 100%. As used herein, the term "substantially free" may mean having no or trace amounts such that the amount of a substance present does not affect the material properties of the composition containing the substance, such that from about 0% by weight to about 5% by weight, or from about 0% by weight to about 1% by weight, or about 5% by weight or less, or less than or equal to about 4.5%, 4%, 3.5%, 3%, 2.5%, 2%, 1.5%, 1%, 0.9%, 0.8%, 0.7%, 0.6%, 0.5%, 0.4%, 0.3%, 0.2%, 0.1%, 0.01%, or about 0.001% by weight or less, or about 0% by weight of the composition is the substance. The term "reactive group" refers to a functional group that can selectively react with another compatible functional group to form a covalent bond after optionally activating one of the functional groups in a certain case. Chemically selective functional groups of interest include, but are not limited to, thiols, maleimides, halogenated maleimides, iodoacetamides, amines, alkyl carboxylates, alkyl sulfonates, carboxylic acid amines, carbamates, carboxylates, N-hydroxysuccinimidyl (NHS), imidoesters, halogens, boronates, boronic acids, hydrazones, carboxylic acids or their active esters, and groups that can react with each other through click chemistry, such as azides and alkyne groups (e.g., cyclooctyne groups), tetrazines and olefin groups (e.g., cyclooctenyl groups), dienes and dienophiles, sulfur (VI) fluoride exchange chemistry (SuFEX), sulfonyl fluorides, and hydroxyls, hydrazides, hydrazines, aldehydes, ketones, azides, alkynes, phosphines, epoxides, etc., or protected groups thereof. The reactive group may be a conjugation tag. The chemoselective functional group may be protected or unprotected. The term "amine reactive group" refers to any group that forms a chemical bond with a primary amine. Interested amine reactive groups include, but are not limited to, isothiocyanates, isocyanates, acyl azides, NHS esters, imidoesters, sulfonyl chlorides, aldehydes, glyoxal, epoxides, oxiranes, carbonates, aryl halides, imidoesters, carbodiimides, anhydrides, and fluorophenyl esters. The amine reactive group can be an NHS ester or an imidoester. In some cases, non-covalent attachment may involve specific binding between two target moieties (e.g., two affinity moieties, such as a hapten and an antibody or a biotin moiety and streptavidin, etc.) In some cases, non-covalent attachment may involve absorption of a substrate. Referring to compounds of the present disclosure, the term "symmetrical" refers to compounds wherein each terminal heterocyclic ring system is identical, and the substituents may be the same or different. In some symmetrical compounds, n=2. With reference to compounds of the present disclosure, the term "asymmetric" refers to compounds wherein each terminal heterocyclic ring system is different. In some asymmetric compounds, n=3. The term "counter ion" refers to an ion that balances the charge of a fluorescent compound according to the present disclosure. The counter ion can be a cation. The counter ion can be an anion. In some cases, the counter ion can be a halide ion, a perchlorate ion, a PF 20 ion, a fluorescein ... 6- , phosphate ions, sulfate ions, etc. The counter ion can be F - , Cl - Br - ,I - 、ClO4 - CF3CO2- 、CH3CO2 - PO4 3- 、SO4 2- 、BF4 - In some cases, the counterion may be Na + , K + Mg ++ , Ca ++ wait. For example, DHP-cyanine dyes or polymer-labeled antibodies according to the present disclosure can be used in flow cytometry as reagents for displaying fluorescent signals. In addition, orthogonal "functional groups" can be included that can be used for bioconjugation of binding partners to the receptor signaling chromophore in the donor-receptor tandem dye or attachment of the receptor signaling chromophore in the donor-receptor tandem dye. As used herein, the term "organic group" refers to any carbon-containing functional moiety. Some examples may include: oxygen-containing groups, such as alkoxy, aryloxy, aralkoxy, oxo (carbonyl) groups; amino groups, including alkylamine amine esters and sulfonamide groups; carboxyl groups, including carboxylic acids, carboxylates and carboxylate esters; sulfur-containing groups, such as alkyl and aryl sulfide groups, thiols, thiol-reactive groups and sulfone groups; maleimide; iodoacetamide; azide groups; alkyne groups; and other heteroatom-containing groups. Some non-limiting examples of organic groups include OR, OOR, OC(O)N(R)2, CN, CF3, OCF3, R, C(O)R, methylenedioxy, ethylenedioxy, N(R)2, N3, S(H)R, SOR, S02R, SO2N(R)2, S03R, C(O)R, C(O)C(O)R, C(O)CH2C(O)R, C(S)R, C(O)OR, OC(O)R, C(O)N(R)2, OC(O)N(R)2, C(S)N(R)2, (CH2) 0-2 N(R)C(O)R,(CH2) 0-2 N(R)N(R)2, N(R)N(R)C(O)R, N(R)N(R)C(O)OR, N(R)N(R)CON(R)2, N(R)SO2R, N(R)SO2N(R)2, N(R)C(O)OR, N(R)C(O)R, N (R)C(S)R, N(R)C(O)N(R)2, N(R)C(S)N(R)2, N(COR)COR, N(OR)R, C(=NH)N(R)2, C(O)N(OR)R, C(=NOR)R and substituted or unsubstituted (C1-C 100 ) hydrocarbyl, wherein R can be hydrogen (in instances comprising other carbon atoms) or a carbon-based moiety, and wherein the carbon-based moiety can be substituted or unsubstituted. The term "heteroatom" as used herein refers to any suitable non-carbon atom inserted between adjacent carbon atoms in an organic group, such as N, O, S, Se, P, B, Al, Si and Ge. The organic group can be cyclic, aromatic or straight or branched chain groups (such as alkyl or olefin). More than one heteroatom (e.g., 1, 2, 3, 4 or 5 heteroatoms) can be inserted between adjacent carbon atoms. The heteroatom can be oxidized to form parts such as, but not limited to, -S(O)- and S(O)2-, sulfinates / esters, sulfonamides. The term "substituted" used herein in conjunction with a molecule or organic group defined herein refers to a state in which one or more hydrogen atoms contained therein are replaced by one or more non-hydrogen atoms (e.g., alkyl, aryl, or functional groups). A "substituted" group may include one or more groups selected from halogen, hydroxy, amino, alkylamino, acylamino, acyl, nitro, cyano, and alkoxy. As used herein, the term "functional group", "functional moiety" or "substituent" refers to a group that can be substituted or substituted onto a molecule or an organic group. Some examples of substituents or functional groups include, but are not limited to, halogens (e.g., F, Cl, Br, and I); alkenes; cycloalkenes; alkynes; cycloalkynes; oxygen atoms in groups such as hydroxyl, alkoxy, aryloxy, aralkyloxy, oxo (carbonyl) groups, carboxyl groups (including carboxylic acids, carboxylates, and carboxylates); sulfur atoms in groups such as thiol groups, alkyl and aryl sulfide groups, sulfoxide groups, sulfone groups, sulfonyl groups, and sulfonamide groups; nitrogen atoms in groups such as amines, hydroxylamines, nitriles, nitro groups, N-oxides, hydrazides, azides, imides, and enamines; and other heteroatoms in a variety of other groups. Some non-limiting examples of substituents that may be bonded to the substituted carbon (or other) atom include F, Cl, Br, I, OR, OC(O)N(R)2, CN, NO, NO2, ONO2, azide, CF3, OCF3, R, O(oxo), S(thiono), C(O), S(O), methylenedioxy, ethylenedioxy, N(R)2, SR, SOR, S02R, S02N(R)2, S03R, C(O)R, C(O)C(O)R, C(O)CH2C(O)R, C(S)R, C(O)OR, OC(O)R, C(O)N(R)2, OC(O)N(R)2, C(S)N(R)2, (CH2) 0-2 N(R)C(O)R,(CH2) 0-2N(R)N(R)2, N(R)N(R)C(O)R, N(R)N(R)C(O)OR, N(R)N(R)CON(R)2, N(R)SO2R, N(R)SO2N(R)2, N(R)C(O)OR, N(R)C(O)R, N(R)C(S)R, N(R)C(O)N(R)2, N(R)C(S)N(R)2, N(COR)COR, N(OR)R, C(=NH)N(R)2, C(O)N(OR)R, and (=NOR)R, wherein R can be hydrogen or a carbon-based moiety; for example, R can be hydrogen, (C1-C 100) hydrocarbon, alkyl, acyl, cycloalkyl, aryl, aralkyl, heterocyclyl, heteroaryl or heteroarylalkyl; or wherein two R groups bonded to a nitrogen atom or adjacent nitrogen atoms may form a heterocyclyl together with one or more nitrogen atoms. The subject fluorescent compound may contain one or more "functional groups" (also referred to as "conjugation tags") that provide for bioconjugation of the dye. In some cases, such functionality can be used to covalently link a biomolecule or binding partner, such as a protein, peptide, affinity ligand, antibody, antibody fragment, polynucleotide or aptamer. In some cases, the functional group or conjugation tag can be selected from amines, carbamates, carboxylates, carboxylates, maleimides, activated esters, N-hydroxysuccinimidyl groups, hydrazines, hydrazides, hydrazones, isothiocyanates, azides, alkynes, cycloalkynes (e.g., alkenes, cycloalkenes (e.g., cyclooctene), tetrazines, aldehydes, thiols, and protected groups thereof for conjugation with substrates, acceptor dyes, functional moieties, or binding partners. The functional group can be protected or unprotected. The functional group can be a reactive or chemoselective functional group that can react with another group by copper-free click chemistry, including strain-promoted azide-alkynecycloaddition (SPAAC) and inverse-electron-demand Diels-Alder (iEDDA) reactions, which enable rapid and specific chemical conjugation. See Kim et al. al., Chem. Sci., 2019, 10, 7835; and Davis et al., J. Org. Chem. 2016, 81, 6816-6819, both of which are incorporated herein by reference in their entirety. The functional group or conjugated tag can be, for example, a cycloalkene (e.g., cyclooctene); an alkyne; a cycloalkyne (e.g., a cyclooctene group, such as, for example, bicyclo[6.1.0]nonyne (BCN)), dibenzocyclooctyne (DBCO); a cycloalkene (e.g., a cyclooctene group, such as, for example, trans-cyclooctene (trans-cyclooctene, TCO); an azide group; or a tetrazine group. As used herein, the term "activated ester" or "activated ester" refers to a carboxyl activating group used in peptide chemistry to promote the facile condensation of a carboxyl group with the free amino group of an amino acid derivative. Descriptions of these carboxyl activating groups can be found in common textbooks of peptide chemistry; for example, KD Kopple, "Peptides and Amino Acids", WA Benjamin, Inc., New York, 1966, pages 50 to 51; and E. Schroder and K. Lubke, "The Peptides"; Vol. 1, Academic Press, New York, 1965, pages 77 to 128. As used herein, the term "ammonium" by itself or as part of another substituent refers to a radical having the formula NHR3 + The cation of wherein each R group is independently hydrogen or a substituted or unsubstituted alkyl, aryl, aralkyl or alkoxy group. Preferably, each R group is hydrogen. As used herein, the term "hydrocarbon" or "hydrocarbyl" refers to a molecule or functional group containing carbon and hydrogen atoms. The term may also refer to a molecule or functional group that typically contains both carbon and hydrogen atoms but in which some or all of the hydrogen atoms are replaced by another functional group. The term "hydrocarbyl" refers to a functional group derived from a straight chain, branched chain, or cyclic hydrocarbon, and may be an alkyl, alkenyl, alkynyl, aryl, cycloalkyl, acyl, or any combination thereof. A hydrocarbyl group may be represented by (C a -C b )alkyl, wherein a and b are integers and means having any number of carbon atoms from a to b. For example, (C1-C4)alkyl means that the alkyl group can be methyl (C1), ethyl (C2), propyl (C3) or butyl (C4), and (C0-C b ) hydrocarbyl means in certain embodiments that there is no hydrocarbyl group. A hydrocarbylene group is a divalent hydrocarbon, for example, a hydrocarbon bonded at two positions. The term "alkyl" used herein refers to a straight or branched saturated aliphatic group having the number of carbon atoms shown, either by itself or as part of another substituent. Alkyl can be an optionally substituted alkyl. For example, C1-C6 alkyl includes but is not limited to methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, isopentyl, hexyl, etc. Other alkyls include but are not limited to heptyl, octyl, nonyl, decyl, etc. Alkyl can contain any number of carbons, such as 1 to 2, 1 to 3, 1 to 4, 1 to 5, 1 to 6, 1 to 7, 1 to 8, 1 to 9, 1 to 10, 2 to 3, 2 to 4, 2 to 5, 2 to 6, 3 to 4, 3 to 5, 3 to 6, 4 to 5, 4 to 6, and 5 to 6 carbons. Alkyl can also refer to an alkyl with up to 20 carbon atoms, such as but not limited to heptyl, octyl, nonyl, decyl, etc. Alkyl can be substituted or unsubstituted. Unless otherwise indicated, a "substituted alkyl" group may be substituted with one or more groups selected from halogen, hydroxy, amino, alkylamino, amido, acyl, nitro, cyano and alkoxy. Alkyl groups are typically monovalent, but may also be divalent, for example when the alkyl group links two moieties together. The term "alkylene" as used herein refers to an alkyl group as defined above to which at least two additional groups are linked (ie, a divalent alkyl group). The two moieties linked to the alkylene group may be linked to the same carbon atom or different carbon atoms of the alkylene group. As used herein, the term "alkoxy" by itself or as part of another substituent refers to an alkyl group as defined above having an oxygen atom connecting the alkyl group to a point of attachment. Alkoxy groups include, for example, methoxy, ethoxy, propoxy, isopropoxy, butoxy, 2-butoxy, isobutoxy, sec-butoxy, tert-butoxy, pentyloxy, hexyloxy, etc. Alkoxy groups may be further substituted with a variety of substituents as described herein. For example, alkoxy groups may be substituted with halogen to form a "halo-alkoxy" group. As used herein, the term "alkene" or "alkenyl" by itself or as part of another substituent refers to a straight chain, branched or cyclic hydrocarbon having at least one double bond between two carbon atoms. Some examples of alkenyl include, but are not limited to, vinyl, propenyl, isopropenyl, 1-butenyl, 2-butenyl, isobutenyl, butadienyl, 1-pentenyl, 2-pentenyl, isopentenyl, 1,3-pentadienyl, 1,4-pentadienyl, 1-hexenyl, 2-hexenyl, 3-hexenyl, 1,3-hexadienyl, 1,4-hexadienyl, 1,5-hexadienyl, 2,4-hexadienyl or 1,3,5-hexatrienyl. Alkenyl is typically monovalent, but may also be divalent, such as when an alkenyl group connects two moieties together. The term "alkyne" or "alkynyl" as used herein, by itself or as part of another substituent, refers to a straight or branched hydrocarbon having at least one triple bond between two carbon atoms. Some examples of alkynyl include, but are not limited to, ethynyl, propynyl, 1-butynyl, 2-butynyl, isobutynyl, sec-butynyl, butadiynyl, 1-pentynyl, 2-pentynyl, isopentenyl, 1,3-pentadiynyl, 1,4-pentadiynyl, 1-hexynyl, 2-hexynyl, 3-hexynyl, 1,3-hexadiynyl, 1,4-hexadiynyl, 1,5-hexadiynyl, 2,4-hexadiynyl or 1,3,5-hexatriynyl. Alkynyl is typically monovalent, but may also be divalent, such as when an alkynyl group connects two moieties together. The term "acyl" as used herein refers to a group comprising a carbonyl moiety, wherein the group is bonded by a carbonyl carbon atom. The carbonyl carbon atom is bonded to a hydrogen to form a "formyl" group, or to another carbon atom (which may be a part of an alkyl, aryl, aralkylcycloalkyl, cycloalkylalkyl, heterocyclyl, heterocyclylalkyl, heteroaryl, heteroarylalkyl, etc.). The acyl group may contain 0 to about 12, 0 to about 20, or 0 to about 40 additional carbon atoms bonded to the carbonyl group. The acyl group may contain a double bond or a triple bond within the meaning of this article. The acyl group may optionally also contain heteroatoms within the meaning of this article. Some examples of acyl groups include, but are not limited to, nicotinoyl (pyridyl-3-carbonyl) acetyl, benzoyl, phenylacetyl, pyridylacetyl, cinnamoyl, and acryloyl, etc. When the group containing the carbon atom bonded to the carbonyl carbon atom contains a halogen, the group is referred to as a "haloacyl" group. An example is trifluoroacetyl. As used herein, the term "aldehyde" by itself or as part of another substituent refers to a compound having a -CHO group. As used herein, the term "aryl" by itself or as part of another substituent refers to a cyclic aromatic hydrocarbon radical that does not contain heteroatoms in the aromatic ring assembly. An "aryl" group can be a monocyclic or fused bicyclic, tricyclic, tetracyclic, pentacyclic or larger aromatic ring assembly containing 6 to 22, 14 to 22, 17 to 22, or 6 to 16 ring carbon atoms. For example, aryl can be, but is not limited to, phenyl, azulenyl, heptalenyl, biphenyl, indacenyl, fluorenyl, phenanthrenyl, triphenylene, pyrenyl, naphthacenyl, The aryl group may include substituted aryl groups. The substituted aryl groups include, for example but not limited to, naphthyl or phenyl, optionally mono- or di-substituted by alkoxy, phenyl, halogen, alkyl or trifluoromethyl, hydroxy, C1-C 12 Alkyl, C2-C 12 Olefins, C2-C 12Alkynes, C3-C 12 Cycloalkyl, C1-C 12 Halogenated alkyl, C1-C 12 Alkoxy, C2-C 18 (Hetero)aryloxy, C2-C 18 (Hetero)arylamino, carboxylate / ester, carboxylic acid, C2-C 12 Alkyl carboxylic acid, C2-C 12 Alkyl carboxylates, C2-C 12 Alkyl carboxylates, C1-C 12 Alkoxy, water-solubilizing group (WSG), functional group, sulfonic acid, sulfonate / ester, C1-C 12 Alkyl sulfonate. In some cases, substituted aryl, such as naphthyl or phenyl, may be mono- or di-substituted by a functional group, WSG (WSG optionally comprising a functional group, alkoxy, halogen or trifluoromethyl). WSG may be a branched WSG, which optionally comprises a functional group, such as, for example, a WSG comprising PEG and a functional group. Preferred aryl is naphthyl, phenyl or phenyl mono- or di-substituted by alkoxy, phenyl, halogen, alkyl or trifluoromethyl, in particular phenyl or phenyl mono- or di-substituted by alkoxy, halogen or trifluoromethyl, and in particular phenyl. The term "monocyclic heteroaryl" refers to an unsubstituted or substituted heteroaryl ring system containing one aryl ring, and wherein the monocyclic ring system contains one or more, two or more, three or more, or four or more heteroatoms. Some non-limiting examples of monocyclic heteroaryl are substituted or unsubstituted pyridinyl, pyranyl, thienyl, furanyl, pyrrolyl, pyrazolyl, imidazolyl, thiazolyl, Azolyl. The term "polycyclic aryl" refers to an unsubstituted or substituted polycyclic ring system comprising 2 to 9, 2 to 8, or 2 to 6 aryl rings with or without fused cycloalkyl or cycloalkenyl rings. Some examples of polycyclic aryl can include fluorene, 9H-fluorene, phenanthrene, dihydrophenanthrene, 9,10-dihydrophenanthrene, naphthalene, anthracene, tetracene, pentacene, and the like. The term "polycyclic heteroaryl" refers to an unsubstituted or substituted polycyclic ring system containing 2 to 9, 2 to 8, or 2 to 6 aryl rings with or without fused cycloalkyl or cycloalkenyl rings, and wherein the polycyclic ring system contains one or more, two or more, three or more, or four or more heteroatoms. Some non-limiting examples of polycyclic heteroaryl systems can include quinoline, benzophenone ... Azoles, benzothiazoles, benzimidazoles, indoles, benzindole, pyridine Benzopyran Thiopyran 6,8-dihydro-5H-naphtho[2,1-f]indole, 4,5-dihydro-3H-naphtho[2,1-e]indole, 6,7-dihydro-3H-naphtho[2,1-g]indole, 5,6-dihydrophenanthro[3,2-d]thiazole, 4,5-dihydrophenanthro[2,1-d]thiazole, 6,7-dihydrophenanthro[4,3-d]thiazole, 5,6-dihydrophenanthro[3,2-d] oxadiazole, 4,5-dihydrophenanthro[2,1-d] oxadiazole, 6,7-dihydrophenanthro[4,3-d] oxadiazole, 5,6-dihydronaphtho[2,1-g]quinoline, 7,8-dihydronaphtho[2,1-h]quinoline, 5,6-dihydronaphtho[2,1-f]quinoline, 5,6-dihydro-8λ2-phenanthro[3,2-d]imidazole, 4,5-dihydro-3λ2-phenanthro[2,1-d]imidazole, 6,7-dihydro-3λ2-phenanthro[4,3-d]imidazole, 5,6-dihydronaphtho[1,2-g]quinoxaline, 5,6-dihydronaphtho[2,1- f]quinoxaline, 7,8-dihydronaphtho[1,2-f]quinoxaline, 5,6-dihydropentaphenanthro[3,2,1-cd:10,11,12-c'd']diindole, 3,8-dihydrophenanthro[2,3-e:7,6-e']diindole, 3,5,6,8-tetrahydrophenanthro[2,3-e:7,6-e']diindole, 1,2,3,5,6,8-hexahydrophenanthro[2,3-e:7,6-e']diindole-1,10-dihydro salt, 3,5,6,8-tetrahydrophenanthro[2,3-e:7,6-e']diindole-ethane (1 / 1), 5,6-dihydropentaphenanthro[3,4-d:10,9-d']bis( azole), 1,2,5,6,9,10-hexahydropentaphen[3,4-d:10,9-d']bis(thiazole)-1,10-dioxadiazole Pentafen[3,4-d:10,9-d']bis(thiazole), 1,2,9,10-tetrahydropentafen[3,4-d:10,9-d']bis(thiazole)-1,10-di 6,7-Dihydrophenanthro[2,3-f:7,6-f']diquinoline-1,12- 6,7-Dihydrophenanthro[2,3-f:7,6-f']diquinoline, 2,6,7,11-tetrahydrobenzo[1,2-g:4,3-g']dichromene, 2,11-dihydrobenzo[1,2-g:4,3-g']dichromene, 5,10-dihydro-6H-naphtho[2,1-g]chromene, 10H-naphtho[2,1-g]chromene, 2,6,7,11-tetrahydrobenzo[1,2-g:4,3-g']bis(thiochromene), 2,11-dihydrobenzo[1,2-g:4,3-g']bis(thiochromene), and the like. The term "arylene" refers to a divalent group derived from an aryl group. The aryl group may be monosubstituted, disubstituted or trisubstituted by one, two or three groups selected from alkyl, alkoxy, aryl, hydroxyl, halogen, cyano, amino, amino-alkyl, trifluoromethyl, alkylenedioxy and oxy-C2-C3-alkylene; all of which are optionally further substituted, for example as defined above; or the aryl group may be 1- or 2-naphthyl; or 1- or 2-phenanthrenyl. Alkylenedioxy is a divalent substituent attached to two adjacent carbon atoms of phenyl, such as methylenedioxy or ethylenedioxy. Oxy-C2-C3-alkylene is also a divalent substituent attached to two adjacent carbon atoms of phenyl, such as oxyethylene or oxypropylene. An example of oxy-C2-C3-alkylene-phenyl is 2,3-dihydrobenzofuran-5-yl. Polycyclic heteroaryl groups may be substituted or unsubstituted. As used herein, the term "aryloxy" by itself or as part of another substituent refers to an O-aryl group, wherein aryl is as defined above. Aryloxy may be unsubstituted or substituted with one or two suitable substituents. The term "phenoxy" refers to an aryloxy group in which the aryl moiety is a benzene ring. As used herein, the term "(hetero)aryloxy" means -O-heteroaryl, wherein heteroaryl is as defined below. The term "(hetero)aryloxy" is used to indicate that the moiety is an aryloxy or heteroaryloxy group. The term "aralkyl" as used herein refers to an alkyl group as defined herein in which a hydrogen bond or carbon bond of the alkyl group is replaced by a bond of an aryl group as defined herein. Representative aralkyl groups include benzyl and phenethyl groups and fused (cycloalkylaryl)alkyl groups, such as 4-ethyl-indanyl. Aralkenyl groups are alkenyl groups as defined herein in which a hydrogen bond or carbon bond of the alkyl group is replaced by a bond of an aryl group as defined herein. The term "amine" as used herein, by itself or as part of another substituent, refers to an alkyl group as defined herein having one or more amino groups. The amino group may be a primary amino group, a secondary amino group, or a tertiary amino group. The alkylamine may be further substituted with a hydroxyl group. Amines useful in the present disclosure include, but are not limited to, ethylamine, propylamine, isopropylamine, ethylenediamine, and ethanolamine. The amino group may connect the point of attachment of the alkylamine to the rest of the compound, be located at the ω position of the alkyl group, or connect at least two carbon atoms of the alkyl group together. It will be appreciated by those skilled in the art that other alkylamines may be useful in the present disclosure. The term "amino" as used herein refers to substituents of the form: -NH2, -NHR, -NR2, -NR3 + (wherein each R is independently selected), and the protonated form of each, except -NR3 +In addition, it cannot be protonated. Therefore, any compound substituted with an amino group can be considered an amine. "Amino" in the sense of this article can be a primary amino group, a secondary amino group, a tertiary amino group or a quaternary amino group. "Alkylamino" groups can include monoalkylamino, dialkylamino or trialkylamino. The term "amide" refers to a functional group having a carbonyl group attached to an amine group, having the general formula RC(=O)NR'R", wherein R, R' and R" represent organic groups or hydrogen atoms. The term "amido" refers to a substituent containing an amide group. As used herein, the term "carbamate" by itself or as part of another substituent refers to a functional group having the structure -NR"CO2R', wherein R' and R" are independently selected from hydrogen, (C1-C8)alkyl and heteroalkyl, unsubstituted aryl and heteroaryl, (unsubstituted aryl)-(C1-C4)alkyl, and (unsubstituted aryl)oxy-(C1-C4)alkyl. Some examples of carbamates include t-Boc, Fmoc, benzyloxy-carbonyl, alloc, methyl carbamate, ethyl carbamate, 9-(2-sulfo)fluorenylmethylcarbamate, 9-(2,7-dibromo)fluorenylmethylcarbamate, Tbfmoc, Climoc, Bimoc, DBD-Tmoc, Bsmoc, Troc, Teoc, 2-phenylethylcarbamate, Adpoc, 2-chloroethylcarbamate, 1,1-dimethyl-2-haloethylcarbamate, DB-t-BOC, TCBOC, Bpoc, t-Bumeoc, Pyoc, Bnpeoc, V-(2-pivaloylamino)-1,1-dimethylethylcarbamate, NpSSPeoc. As used herein, the term "carboxylic acid" by itself or as part of another substituent refers to the structure R-COOH, where R is a carbon-containing atom group. As used herein, the term "carboxylate" by itself or as part of another substituent refers to the conjugate base of a carboxylic acid, which can generally be represented by the formula RCOO. - Represents. For example, the term "magnesium carboxylate" refers to the magnesium salt of a carboxylic acid. The term "carboxylate" as used herein, by itself or as part of another substituent, refers to a compound derived from a carboxylic acid, which can generally be represented by the formula RCOOR', where R' can be an alkyl, alkene, alkyne, haloalkyl, heteroalkyl, cycloalkyl, aryl, heteroaryl, (unsubstituted aryl) alkyl and (unsubstituted aryl) oxygen-alkyl or other carbon-containing atom groups. R' may optionally contain a functional group. The term "cycloalkyl" as used herein by itself or as part of another substituent refers to a saturated or partially unsaturated monocyclic, fused bicyclic or bridged polycyclic ring assembly containing 3 to 12 ring atoms or the indicated number of atoms. Cycloalkyl can contain any number of carbon atoms, such as C3-6 , C 4-6 , C 5-6 , C 3-8 , C 4-8 , C 5-8 , C 6-8 , C 3-9 , C 3-10 , C 3-11 and C 3-12 . Saturated monocyclic cycloalkyl rings include, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and cyclooctyl. Saturated bicyclic and polycyclic cycloalkyl rings include, for example, norbornane, [2.2.2]bicyclooctane, decalin, and adamantane. Cycloalkyl groups may also be partially unsaturated, having one or more double or triple bonds in the ring. Representative partially unsaturated cycloalkyl groups include, but are not limited to, cyclobutene, cyclopentene, cyclohexene, cyclohexadiene (1,3- and 1,4-isomers), cycloheptene, cycloheptadiene, cyclooctene, cyclooctadiene (1,3-, 1,4-, and 1,5-isomers), norbornene, and norbornadiene. When the cycloalkyl group is a saturated monocyclic C 3-8 When cycloalkyl is a saturated monocyclic ring C 3-6 When a cycloalkyl group is present, some exemplary groups include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl. The cycloalkyl group may be substituted or unsubstituted. Unless otherwise indicated, a "substituted cycloalkyl" group may be substituted by one or more groups selected from halogen, hydroxy, amino, alkylamino, acylamino, acyl, nitro, cyano, and alkoxy. The term "low cycloalkyl" refers to a cycloalkyl group having three to seven carbon atoms, including, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and cycloheptyl. Monocyclic rings include, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and cyclooctyl. Bicyclic and polycyclic rings include, for example, norbornane, decalin, and adamantane. For example, C 3-8 Cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cyclooctyl and norbornane.The polycyclic ring systems may be substituted or unsubstituted. The term "cycloalkylene" as used herein refers to a cycloalkyl group as defined above (ie, a divalent cycloalkyl group) that is linked to at least two additional groups. The two moieties linked to the cycloalkylene group may be linked to the same atom or different atoms of the cycloalkylene group. The term "haloalkyl" as used herein, by itself or as part of another substituent, refers to an alkyl group as defined above in which some or all of the hydrogen atoms are replaced by halogen atoms. Halogen (halogen) (halo) preferably means chloro or fluoro, but may also be bromo or iodo. For example, haloalkyl includes trifluoromethyl, fluoromethyl, 1,2,3,4,5-pentafluoro-phenyl, etc. The term "perfluoro" defines a compound or group having at least two available hydrogens replaced by fluorine. For example, perfluorophenyl refers to 1,2,3,4,5-pentafluorophenyl, perfluoromethane refers to 1,1,1-trifluoromethyl, and perfluoromethoxy refers to 1,1,1-trifluoromethoxy. As used herein, the term "halogen," by itself or as part of another substituent, refers to fluorine, chlorine, bromine, and iodine. As used herein, the term "heteroaryl" by itself or as part of another substituent refers to a monocyclic or fused polycyclic, such as a bicyclic, tricyclic, tetracyclic or pentacyclic aromatic ring assembly, for example, containing 5 to 22, 14 to 22, 17 to 22, 6 to 16 or 5 to 16 ring atoms, of which 1 to 4 ring atoms may be heteroatoms, such as N, O or S. Additional heteroatoms may also be useful, including but not limited to B, Al, Si or P. Heteroaryl may be substituted or unsubstituted. Substituted heteroaryl may contain one or more KR 13 , halogen, OC 1-6 Alkyl, SC 1-6 Alkyl, O-aryl, S-aryl, NHC 1-6 Alkyl, Ph-NCS, Ph-CO2H, Ph-(CH2) 1-4 CO2H substituents. The heteroatoms may be oxidized to form moieties such as, but not limited to, -S(O)- and -S(O)2-. For example, heteroaryl groups may include pyridyl, indolyl, indazolyl, quinoxalinyl, quinolyl, isoquinolyl, benzothiophenyl, benzofuranyl, furanyl, pyrrolyl, thiazolyl, benzothiazolyl, Azolyl, iso Oxyl, triazolyl, tetrazolyl, pyrazolyl, imidazolyl, thienyl or any other substituted group, especially a group monosubstituted or disubstituted by, for example, alkyl, nitro or halogen. Pyridyl may represent 2-, 3- or 4-pyridyl, advantageously 2- or 3-pyridyl. Thienyl may represent 2- or 3-thienyl. Quinolyl may preferably represent 2-, 3- or 4-quinolyl. Isoquinolyl may preferably represent 1-, 3- or 4-isoquinolyl. Benzopyranyl, benzothiopyranyl preferably represent 3-benzopyranyl or 3-benzothiopyranyl, respectively. Thiazolyl may preferably represent 2- or 4-thiazolyl, and most preferably, 4-thiazolyl. Triazolyl may preferably represent 1-, 2- or 5-(1,2,4-triazolyl). Tetrazolyl is preferably 5-tetrazolyl. The heteroaryl group may include an aryloxy group or an arylamino group. In some embodiments, the heteroaryl group is pyridyl, indolyl, quinolyl, pyrrolyl, thiazolyl, isothioyl, oxazolyl, triazolyl, tetrazolyl, pyrazolyl, imidazolyl, thienyl, furyl, benzothiazolyl, benzofuranyl, isoquinolyl, benzothiophenyl, oxazolyl, indazolyl or any substituted group, especially mono- or di-substituted groups. As used herein, the term "heteroalkyl" or "heteroalkoxy" by itself or as part of another substituent refers to an alkyl or alkoxy group, preferably a C1-C 12 Alkyl or C1-C 12 Alkoxy, wherein C is substituted with a heteroatom (e.g., N, O, or S). For example, heteroalkyl or heteroalkoxy may include ethers, thioethers, and alkylamines. Additional heteroatoms may also be useful, including but not limited to B, Al, Si, or P. Heteroatoms may be oxidized to form moieties such as, but not limited to, -S(O)-, -S(O)2-, sulfinic acid / salt esters, and sulfonamides. The heteroatom moiety of the heteroalkyl may replace the hydrogen atoms of the alkyl to form hydroxyl, thio, or amino. Alternatively, the heteroatom moiety may be a linking atom, or may be inserted between two carbon atoms. The term "heteroalkylene" as used herein refers to a heteroalkylene group as defined above (ie, a divalent heteroalkylene group) that is linked to at least two additional groups. The two moieties linked to the heteroalkylene group may be linked to the same atom or different atoms of the heteroalkylene group. As used herein, the term "(hetero)arylamino" by itself or as part of another substituent refers to an amine group substituted with an aryl group (e.g., -NH-aryl). An arylamino group can also be an aryl group substituted with an amine group (e.g., -aryl-NH2). An arylamino group can be substituted or unsubstituted. In some embodiments, the substituents for the aryl, heteroaryl, and heteroalkylene groups are different and are selected from: -halogen, -OR', -OC(O)R', -C(O)R', -NR'R", -SR', -R', -CN, -NO2, -CO2R', -CONR'R", -C(O)R', -OC(O)NR'R", -NR"C(O)R', -NR"C(O)2R', -NR'-C(O)NR"R"', -NH-C(NH2)=NH, -NR'C(NH2)=NH, - NH-C(NH2)=NR', -S(O)R', -S(O)2R', -S(O)2NR'R", -N3, -CH(Ph)2, perfluoro(C1-C4)alkoxy and perfluoro(C1-C4)alkyl, ranging from zero to the total number of open valences on the aromatic ring system; and wherein R', R" and R'" are independently selected from hydrogen, (C1-C5)alkyl and heteroalkyl, unsubstituted aryl and heteroaryl, (unsubstituted aryl)-(C1-C4)alkyl and (unsubstituted aryl)oxy-(C1-C4)alkyl. Two substituents on adjacent atoms of an aryl or heteroaryl ring may optionally be replaced by groups of the formula -TC(O)-(CH2) q -U-, wherein T and U are independently -NH-, -O-, -CH2- or a single bond, and q is an integer from 0 to 2. Alternatively, two substituents on adjacent atoms of the aryl or heteroaryl ring may be optionally replaced by a substituent of the formula -A-(CH2) r -B-, wherein A and B are independently -CH2-, -O-, -NH-, -S-, -S(O)-, -S(O)2-, -S(O)2NR'- or a single bond, and r is an integer from 1 to 3. One of the single bonds of the new ring thus formed may optionally be replaced by a double bond. Alternatively, two substituents on adjacent atoms of the aryl or heteroaryl ring may optionally be replaced by a double bond of the formula -(CH2) s -X'-(CH2) t -, wherein s and t are independently integers from 0 to 3, and X' is -O-, -NR'-, -S-, -S(O)-, -S(O)2- or -S(O)2NR'-. The substituent R' in -NR'- and -S(O)2NR'- is selected from hydrogen or unsubstituted (C1-C6) alkyl. As used herein, the term "oligoether" is understood to mean an oligomer comprising a structural repeating unit having ether functionality. As used herein, "oligomer" is understood to mean a molecule comprising one or more identifiable structural repeating units of the same or different formula. As used herein, the term "polyethylene glycol", "PEG", "polyethylene oxide" or "PEO" refers to a polyethylene glycol having the formula -(CH2-CH2-O-)n - or its derivatives described ethylene glycol monomer units of the biocompatible water-soluble straight-chain polymer family. In some embodiments, "n" is 5000 or less, such as 1000 or less, 500 or less, 200 or less, 100 or less, 50 or less, 40 or less, 30 or less, 20 or less, 15 or less, such as 3 to 15 or 10 to 15. It should be understood that the PEG polymeric group can have any convenient length and can contain a variety of end groups and / or other substituent groups, including but not limited to alkyl, alkoxy, aryl, hydroxyl, amino, acyl, carboxylic acid, carboxylate, acyloxy and amido end groups and / or substituent groups. The PEG groups used herein include but are not limited to PEG, modified PEG, connected PEG, amide PEG, sulfonamide PEG, phosphoamido-PEG, alkylsulfonamide-PEG and alkoxysulfonamide-PEG. It will be appreciated that the PEG polymeric moiety may be of any convenient length and may contain a variety of terminal and / or other substituent groups, including but not limited to alkyl, aryl, hydroxy, amino, acyl, acyloxy, and amido terminal and / or substituent groups. PEG groups suitable for use in the subject compounds include those described by S. Zalipsky in "Functionalized poly(ethylene glycol) for preparation of biologically relevant conjugates", Bioconjugate Chemistry 1995, 6(2), 150-165; by Zhu et al in "Water-Soluble Conjugated Polymers for Imaging, Diagnosis, and Therapy", Chem. Rev., 2012, 112(8), pp. 4687 to 4735; by JM Harris in "Poly(ethylene glycol) Chemistry: Biotechnical and Biomedical Applications", Plenum Press, New York, NY (1992) and by JM Harris and S Zalipsky in "Poly(ethylene glycol) Chemistry and Biological Applications", ACS (1997). In some cases, PEG and modified PEG moieties may be those taught, for example, in the following international patent applications: WO90 / 13540,WO92 / 00748,WO92 / 16555,WO 94 / 04193,WO 94 / 14758,WO 94 / 17039,WO94 / 18247,WO94 / 28937,WO95 / 11924,WO96 / 00080,WO96 / 23794,WO 98 / 07713,WO98 / 41562,WO98 / 48837,WO99 / 30727,WO99 / 32134,WO99 / 33483,WO 99 / 53951,WO 01 / 26692, WO95 / 13312, WO96 / 21469, WO97 / 03106, WO99 / 45964 U.S. Patent No. 4,179,337; 5,075,046; 5,089,261, 5,100,992, 5,134,192, 5,166,309; 5,171,264; 5,213,891, 5,219,564; 5,275,838; 5,281, 698; 5,298,643; 5,312,808; 5,321,095; 5,324,844; 5,349,001; 5,352,756; 5,405,877; 5,455,027; 5,446,090; 5,470,829; 5,478,805; 5,567,422; 5,605,976; 5,612,460; 5,614,549; 5,618,528; 5,672,662; 5,637,749; 5,643,575; 5,650,388; 5,681,567; 5,686,110; 5,730,990; 5,739,208; 5,756,593; 5,808,096; 5,824,778; 5,824,784; 5,840,900; 5,874,500; 5,880,131; 5,900,461; 5,90 2,588; 5,919,442; 5,919,455; 5,932,462; 5,965,119; 5,965,566; 5,985,263; 5,990,237; 6,011,042; 6,013,283; 6,077,939; 6,113,906; 6,127,355; 6,177,087; 6,180,095; 6,194,580; 6,214,966 each of which is incorporated herein by reference). As used herein, the term "sulfonate functional group" or "sulfonate" by itself or as part of another substituent refers to both the free sulfonate anion (-S(=O)2O-) and its salts. Thus, the term sulfonate encompasses sulfonates such as sodium sulfonate, lithium sulfonate, potassium sulfonate, and ammonium sulfonate. The term "sulfonamide" used herein itself or as a part of another substituent refers to a group of formula -SO2NR2, wherein each R can be independently such as a water-soluble moiety, hydrogen, alkyl, alkene, alkyne, cycloalkyl, haloalkyl, aryl or functional group, and can include a carboxylic acid group. R can be a water-soluble polymer, including but not limited to a polymer comprising 6 or more monomeric units, a nonionic water-soluble polymer, such as PEG, a modified PEG such as carboxylic acid, carboxylate or alkoxy (such as O methyl or O ethyl) end-capped. "Sulfonamide" is connected to another molecule by a joint or a key. "Sulfonamide" can be such as sulfonamide-PEG, alkylsulfonamide, alkoxysulfonamide, alkylsulfonamide PEG, alkoxysulfonamide PEG, alkylsulfonamide PEG carboxylate, alkoxysulfonamide PEG carboxylate. The term "sulfonylamino" as used herein refers to a group of formula -SO2NR- by itself or as part of another substituent, wherein R can be, for example, a water-soluble moiety, hydrogen, an alkyl, an alkene, an alkyne, a cycloalkyl, a haloalkyl, an aryl or a functional group, and can include a carboxylic acid group. R can be a water-soluble polymer, including but not limited to a polymer comprising 6 or more monomeric units, a nonionic water-soluble polymer, such as PEG, a modified PEG terminated with a carboxylic acid or a carboxylate. "Sulfonylamino" can be connected to another molecule by a joint or a bond. "Sulfonylamino" can be, for example, sulfonylamino-PEG, alkylsulfonylamino, alkoxysulfonylamino, alkylsulfonylamino PEG, alkoxysulfonylamino PEG, alkylsulfonylamino PEG carboxylate, alkoxysulfonylamino PEG carboxylate. The term "sulfenamide" used herein itself or as a part of another substituent refers to a group of formula -SONR2, wherein each R can be independently such as a water-soluble moiety, hydrogen, an alkyl, an alkene, an alkyne, a cycloalkyl, a haloalkyl, an aryl or a functional group, and can include a carboxylic acid group. R can be a water-soluble polymer, including but not limited to a polymer comprising 6 or more monomeric units, a nonionic water-soluble polymer, such as PEG, a modified PEG terminated with a carboxylic acid or a carboxylate." sulfenamide " can be connected to another molecule by a joint or a key." sulfenamide " can be such as sulfenamide-PEG, alkylsulfenamide, alkoxysulfenamide, alkylsulfenamide PEG, alkoxysulfenamide PEG, alkylsulfenamide PEG carboxylate, alkoxysulfenamide PEG carboxylate. As used herein, the terms "hydrazine" and "hydrazide" by themselves or as part of another substituent refer to compounds containing singly bonded nitrogens, one of which is a primary amine functional group. For example, the term "hydrazine" refers to a moiety having a -NHNH2 structure. As used herein, the term "thiol" by itself or as part of another substituent refers to a compound containing a functional group consisting of a sulfur-hydrogen bond. The general chemical structure of a thiol functional group is R-SH, where R represents an alkyl, alkene, aryl or other carbon-containing atom group. The term "silyl" as used herein, by itself or as part of another substituent, refers to Si(R z )3, where each R z are independently alkyl, aryl or other carbon-containing atom groups. As used herein, the term "diazonium salt" by itself or as part of another substituent refers to a diazonium salt having the structure R-N2 + X' - A radical of an organic compound wherein R can be any organic radical (eg, an alkyl or aryl radical) and X' is an inorganic or organic anion (eg, a halogen). As used herein, the term "triflate" by itself or as part of another substituent also refers to trifluoromethanesulfonate, a group having the formula CF3SO3. As used herein, the term "boronic acid" by itself or as part of another substituent refers to the structure -B(OH)2. Those skilled in the art recognize that at different stages of the quencher synthesis, boronic acid may exist as a boric acid ester. Boric acid is intended to include such esters. As used herein, the term "boronic ester" or "boronate ester" refers to a boronic acid ester comprising -B(Z 1 )(Z 2 ) moiety, wherein Z 1 and Z 2 Together they form a moiety wherein the atom to which boron is attached in each case is an oxygen atom. In some embodiments, the borate ester moiety is a 5-membered ring. In some other embodiments, the borate ester moiety is a 6-membered ring. In some other embodiments, the borate ester moiety is a mixture of 5-membered and 6-membered rings. As used herein, the term "maleimide" by itself or as part of another substituent refers to the structure Wherein R can be, for example, a water-soluble moiety, hydrogen, an alkyl group, an alkene, an alkyne, a cycloalkyl group, a haloalkyl group, an aryl group or other groups, and can include a carboxylic acid group. R can be a water-soluble polymer, including but not limited to a polymer comprising 6 or more monomeric units, a nonionic water-soluble polymer, PEG, a modified PEG terminated with a carboxylic acid or a carboxylate. As used herein, the term "hydrazone" by itself or as part of another substituent refers to the structure Wherein R can be, for example, a water-soluble moiety, hydrogen, an alkyl group, an alkene, an alkyne, a cycloalkyl group, a haloalkyl group, an aryl group or other groups, and can include a carboxylic acid group. R can be a water-soluble polymer, including but not limited to a polymer comprising 6 or more monomeric units, a nonionic water-soluble polymer, PEG, a modified PEG terminated with a carboxylic acid or a carboxylate. As used herein, the term "azide" by itself or as part of another substituent refers to the structure -N3. The term "tetrazine" as used herein, by itself or as part of another substituent, refers to a compound having the molecular formula C2H2N4, which consists of a six-membered heteroaromatic ring structure having four nitrogen atoms. The term "tetrazine" includes all isomers thereof, i.e., 1,2,3,4-tetrazine, 1,2,3,5-tetrazine and 1,2,4,5-tetrazine. As used herein, the term "N-hydroxysuccinimidyl" by itself or as part of another substituent refers to the structure Wherein R can be, for example, a water-soluble moiety, hydrogen, an alkyl group, an alkene, an alkyne, a cycloalkyl group, a haloalkyl group, an aryl group or other groups, and can include a carboxylic acid group. R can be a water-soluble polymer, including but not limited to a polymer comprising 6 or more monomeric units, a nonionic water-soluble polymer, PEG, a modified PEG terminated with a carboxylic acid or a carboxylate. As used herein, the term "phosphamide" by itself or as part of another substituent refers to the structure Wherein R can be, for example, a water-soluble moiety, hydrogen, an alkyl group, an alkene, an alkyne, a cycloalkyl group, a haloalkyl group, an aryl group or other groups, and can include a carboxylic acid group. R can be a water-soluble polymer, including but not limited to a polymer comprising 6 or more monomeric units, a nonionic water-soluble polymer, PEG, a modified PEG terminated with a carboxylic acid or a carboxylate. As used herein, the term "phosphonamidate" by itself or as part of another substituent refers to the structure In some embodiments, the present invention relates to a phosphonoacid ester, wherein R can be, for example, a water-soluble moiety, hydrogen, an alkyl group, an alkene group, an alkyne group, a cycloalkyl group, a haloalkyl group, an aryl group or other groups, and can comprise a carboxylic acid group. R can be a water-soluble polymer, and it includes but is not limited to a polymer, a nonionic water-soluble polymer, a PEG, a modified PEG with a carboxylic acid or a carboxylate end-capped polymer comprising 6 or more monomeric units." phosphonoacid esters" can be connected with another molecule by a joint or a key." phosphonoacid esters" can be, for example, phosphonoacid ester-PEG, alkyl phosphonoacid esters, alkoxy phosphonoacid esters, alkyl phosphonoacid esters PEG, alkoxy phosphonoacid esters PEG, alkyl phosphonoacid esters PEG carboxylates, alkoxy phosphonoacid esters PEG carboxylates. As used herein, the term "phosphinamide" by itself or as part of another substituent refers to the structure Wherein R can be for example water-soluble part, hydrogen, alkyl, alkene, alkynes, cycloalkyl, halogenated alkyl, aryl or other groups, and can comprise carboxylic acid radical.R can be water-soluble polymer, it includes but not limited to comprise the polymer of 6 or more monomeric units, nonionic water-soluble polymer, PEG, with the modified PEG of carboxylic acid or carboxylate end-blocking." phosphinamide " can be connected with another molecule by joint or key." phosphinamide " can be for example phosphinamide-PEG, alkyl phosphinamide, alkoxy phosphinamide, alkyl phosphinamide PEG, alkoxy phosphinamide PEG, alkyl phosphinamide PEG carboxylate, alkoxy phosphinamide PEG carboxylate. As used herein, the term "fluorescence" refers to a compound that emits light of (usually) a different wavelength when the compound is irradiated with light of the wavelength that it absorbs. Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. In most cases, the emitted light has a longer wavelength than the absorbed light. The term "absorbance maximum" or "Absλmax" or "maxλabs" refers to the wavelength of maximum absorbance as measured by UV-visible spectroscopy. The term "excitation wavelength" or "λex" refers to the wavelength at which a compound can be excited to induce fluorescence emission, which is not necessarily necessarily at Absλmax. The term "chromophore" refers to a molecular entity or portion thereof consisting of an atom or group of atoms in which the electronic transitions responsible for a given spectral band are approximately located. In some cases, the "chromophore" itself can be fluorescent. The terms "fluorescent chromophore" and "fluorescent dye" used herein are used interchangeably and refer to a compound having a structure that can collect light with a specific absorption maximum wavelength and convert it into emitted light of a longer emission maximum wavelength. The chromophore may have a reactive group (e.g., a carboxylate / ester moiety, an amino moiety, a haloalkyl moiety, etc.) to which it can be covalently bonded. Some examples of suitable chromophores are, but are not limited to, those described in U.S. Patent Nos. 7,687,282; 7,671,214; 7,446,202; 6,972,326; 6,716,979; 6,579,718; 6,562,632; 6,399,392; 6,316,267; 6,162,931; 6,130,101; 6,005,113 ; 6,004,536; 5,863,753; 5,846,737; 5,798,276; 5,723,218; 5,696,157; ​​5,658,751; 5,656,449; 5,582,977; 5,576,424; 5,573,909; and 5,187,288, which are incorporated herein by reference in their entirety. The term "moiety" refers to a group that is part of a molecule, which can be a functional group, or a part of a molecule having multiple groups that share common structural and / or functional aspects. Some examples of groups or moieties include, but are not limited to, linker moieties, functional groups, water-soluble moieties, PEG moieties according to the present disclosure. The term "joint", "connection" or "linkage" refers to a connecting portion that connects two groups and has a main chain length of 100 atoms or less. The joint or connector can be a covalent bond connecting two groups or a chain length of 1 to 100 atoms, such as a chain length of 1, 2, 3, 4, 5, 6, 8, 10, 12, 14, 16, 18, 20 or more carbon atoms, wherein the joint can be straight, branched, cyclic or a single atom. In some embodiments, the joint is a branched joint, which refers to a connecting portion that connects three or more groups. In some embodiments, the joint backbone comprises a connecting functional group, such as an ether, a thioether, an amino group, an amide, a carbonyl group, an acyl group, a sulfonamide, a sulfenamide, a disulfonamide, a disulfenamide, a sultam, an amide, a secondary amine, a phosphamide, a phosphinamide, a phosphinamide ester, a selenoamide, a selenonamide, a carbamate, a thiocarbamate, a urea, a thiourea, an ester, a thioester or an imine. In some embodiments, the joint main chain comprises a connection functional group, such as amino, amide, carbonyl, sulfonamide, sulfenamide, disulfonamide, disulfenamide, sultam, amide, secondary amine, phosphamide, phosphinamide, phosphoamidate, selenoamide or selenamide. In some cases, one, two, three, four or five or more carbon atoms of the joint main chain can be optionally replaced by sulfur, nitrogen or oxygen heteroatoms. In some embodiments, the joint main chain comprises a connection functional group, such as ether, thioether, amino, amide, carbonyl, acyl, sulfonamide, carbamate / ester, thiocarbamate / ester, urea, thiourea, ester, thioester or imines. The key between the main chain atoms can be saturated or unsaturated, and in some cases, there is no more than one, two or three unsaturated bonds in the joint main chain. The joint can include one or more substituent groups, such as alkyl, aryl or alkenyl. The linker may include, but is not limited to, polyethylene glycol, ethers, thioethers, tertiary amines, alkyl groups, which may be linear or branched, such as methyl, ethyl, n-propyl, 1-methylethyl (isopropyl), n-butyl, n-pentyl, 1,1-dimethylethyl (tert-butyl), etc. The linker backbone may contain a cyclic group, such as an aryl, heterocyclic or cycloalkyl group, wherein 2 or more atoms of the cyclic group, such as 2, 3 or 4 atoms, are contained in the backbone. The linker may be cleavable or non-cleavable. The linker moiety can be connected to "L" or "A" as taught in U.S. Patent No. 11,584,825B2, which is incorporated herein by reference in its entirety. The linker moiety can comprise a covalent bond, an alkoxyl group, a sulfonamide, a disulfonamide, a selenoamide, a sulfenamide, a sultam, a disulfenamide, an amide, a carbonyl group, a selenite, a phosphamide, a phosphinamide, a phosphinamide ester, or a secondary amine. As described therein, and as each refers to a linker moiety, the term "sulfonamide" refers to the moiety -S(O)2NR-; the term "disulfonamide" refers to the moiety -S(O)2NRS(O)2-; the term "selenoamide" refers to the moiety -Se(O)2NR-; the term "sulfenamide" refers to the moiety -S(O)NR2; the term "disulfenamide" refers to the moiety -S(O)NRS(O)-; the term "selenonamide" refers to the moiety -Se(O)NR-; the term "phosphamide" refers to the moiety -NR-PR(O)NR-; the term "phosphinamide" refers to the moiety -PR(O)NR-; and the term "phosphonamidate" refers to the moiety -O-PR(O)NR-; and the term "sultainamide" refers to a cyclic sulfonamide (e.g., wherein the R group is bonded to the sulfur atom through an alkylene moiety); wherein for each term, the R group is independently H, alkyl, haloalkyl, or aryl. The subject water-soluble fluorescent polymer is characterized in that the end on the polymer chain of conjugation may include a functional group provided for biological conjugation. In some cases, such functionality is called an end joint or end group. With these end joints, a covalent bond can be formed to connect biomolecules (such as proteins, peptides, affinity ligands, antibodies, antibody fragments, polynucleotides or aptamers). For example, it is found that antibodies labeled with polymer dyes are used as reagents showing high brightness in flow cytometry. In addition, orthogonal functional groups can be installed along the polymer chain of conjugation, which can be used for biological conjugation or connection of receptor signaling chromophores in donor receptor polymer tandem dyes. The phrase "conjugated water-soluble fluorescent polymer" refers to a water-soluble fluorescent polymer having a binding partner conjugated thereto. In the chemical structure, Represents a single bond or a double bond. In the chemical structure, represents an optional aryl group. The phrase "binding partner" or "binding member" refers to any molecule or molecular complex that is capable of specifically binding to a target analyte. Binding partners of the present disclosure include, for example, proteins (e.g., antibodies or antibody fragments), carbohydrates (e.g., polysaccharides), oligonucleotides, polynucleotides, lipids, affinity ligands, aptamers, and the like. In some embodiments, the binding partner is an antibody or a fragment thereof. In the context of the present disclosure, specific binding refers to a binding reaction that determines the presence of a target analyte in the presence of a heterogeneous population. Thus, under certain assay conditions, a specific binding partner preferentially binds to a specific protein or an isoform of a specific protein, and does not bind to other proteins or other isoforms present in the sample in significant amounts. In some cases, the antibody comprises intravenous immunoglobulin (IVIG) and / or an antibody from (e.g., enriched from, purified from (e.g., affinity purified from)) IVIG. IVIG is a blood product that contains IgG (immunoglobulin G) pooled from the plasma (e.g., in some cases without any other proteins) of many (e.g., sometimes more than 1,000 to 60,000) normal and healthy blood donors. IVIG is commercially available. For example, some aspects of IVIG are described in U.S. Patent Application Publication Nos. 2010 / 0150942; 2004 / 0101909; 2013 / 0177574; 2013 / 0108619 and 2013 / 0011388. In some cases, the antibody is a monoclonal antibody of a defined subclass (e.g., IgG1, IgG2, IgG3, or IgG4). If a combination of antibodies is used, the antibodies may be from the same subclass or from different subclasses. For example, the antibody may be an IgG1 antibody. In some embodiments, the monoclonal antibody is humanized. The phrase "water-soluble fluorescent polymer complex" refers to a water-soluble fluorescent polymer of the present disclosure conjugated to a binding partner. The phrase "protected group" (also referred to as "protecting group") refers to a reversibly formed derivative of an existing functional group in a molecule that is attached to reduce reactivity so that the protected functional group does not react under the synthetic conditions to which the molecule is subjected. Some examples of amine protecting groups include, but are not limited to, benzyloxycarbonyl; 9-fluorenylmethoxycarbonyl (Fmoc); tert-butyloxycarbonyl (Boc); allyloxycarbonyl (Alloc); p-toluenesulfonyl (Tos); 2,2,5,7,8-pentamethylchroman-6-sulfonyl (Pmc); 2,2,4,6,7-pentamethyl-2,3-dihydrobenzofuran-5-sulfonyl (Pbf); mesityl-2-sulfonyl (Mts); 4-methoxy-2,3,6-trimethylphenylsulfonyl (Mtr); acetamido; phthalimido, and the like. It can be used, for example, as described by Green and Wuts (Protective Groups in Organic Synthesis, 4 th These and other protecting groups for amines, carboxylic acids, alcohols, and other functional groups are added to and removed from the polymers of the present disclosure using known techniques described in . The term "sample" refers to a material or material mixture comprising one or more target analytes, in some cases in liquid form. In some embodiments, the term refers to any plant, animal or bacterial material comprising cells or producing cell metabolites when used in its broadest sense, such as tissue or fluid (including but not limited to plasma, serum, cerebrospinal fluid, lymph, tears, saliva and tissue sections) separated from an individual or tissue or fluid separated from an in vitro cell culture component, and samples from the environment. The term "sample" may also refer to a "biological sample". The term "biological sample" used herein refers to a subgroup (such as body fluids, including but not limited to blood, mucus, lymph, synovial fluid, cerebrospinal fluid, saliva, amniotic fluid, amniotic cord blood, urine, vaginal fluid and semen) of a whole organism or its tissues, cells or components. "Biological sample" can also refer to a homogenate, lysate or extract prepared from a whole organism or a subset of its tissues, cells or components or a fraction or part thereof, including but not limited to plasma, serum, spinal fluid, lymph fluid, exocrine secretions of the skin, respiratory tract, intestinal tract and urogenital tract, tears, saliva, milk, blood cells, tumors and organs. In certain embodiments, the sample has been taken out from an animal or plant. The biological sample may include cells. The term "cell" is used in its conventional sense to refer to the basic structural unit of a living organism (both eukaryotic and prokaryotic) having at least a nucleus and a cell membrane. In certain embodiments, the cell includes a prokaryotic cell, such as a prokaryotic cell from a bacterium. In other embodiments, the cell includes a eukaryotic cell, such as a cell obtained from a biological sample from an animal, a plant or a fungus. The term "substrate" refers to a solid material having a variety of configurations. The substrate can be, for example, a sheet, a bead, or other structure, such as a plate with holes, a polymer, a particle, a semiconductor surface, a nanotube, a fiber mesh, a hydrogel, a porous matrix, a needle, a microarray surface, a chromatography support, etc. In some cases, the substrate is selected from a particle, a flat solid substrate, a fiber mesh, a hydrogel, a porous matrix, a needle, a microarray surface, and a chromatography support. The term "water" as used herein refers to any aqueous solution that is mainly water and compatible with physiological conditions. In some cases, the aqueous solution comprises more than 50% water, such as more than 60% water, more than 70% water, more than 80% water, more than 90% water or more than 95% water. The term "water" includes, for example, biological buffers and other aqueous solutions, which may include additives such as salts, detergents, stabilizers and other water-soluble components (such as sugars, proteins, amino acids and nucleotides). In some cases, "water" can be an aqueous solution comprising up to 10% miscible organic solvents (for example, up to 10% DMSO in water). The term "water" does not include pure solvents or solvent combinations different from water, such as pure alcohols, such as pure methanol or ethanol; pure ethers, such as pure ether or tetrahydrofuran; or any other pure solvents that may be miscible with water or not miscible with water. As used herein, the term "water-soluble moiety" by itself or as part of another group refers to any hydrophilic group that is fully solvated in an aqueous environment (e.g., such as under physiological conditions) and is capable of increasing the water solubility of the molecule to which it is attached. The increase in water solubility of the molecule may vary depending on the portion to which it is attached. In some cases, the increase in water solubility (compared to the solubility of the molecule without the attached portion) is 2 times or more, 5 times or more, 10 times or more, 25 times or more, 50 times or more, or 100 times or more. "Water-soluble moieties" include the following moieties, such as, but not limited to: PEG groups, carboxyl groups (including but not limited to carboxylic acids and carboxylates), polyvinyl alcohol, ethylene glycol, peptides, polyphosphates, polyols, sulfonates, phosphonates, borates, amines, ammonium, sulfonium, Alcohol, polyol, oxazolines, zwitterionic derivatives, carbohydrates, nucleotides, polynucleotides, substituted PEG groups, substituted carboxyl groups (including but not limited to substituted carboxylic acids and substituted carboxylates), substituted glycols, substituted peptides, substituted polyphosphates, substituted polyols, substituted sulfonates, substituted phosphonates, substituted borates, substituted amines, substituted ammoniums, substituted sulfoniums, substituted alcohols, substituted zwitterionic derivatives, substituted carbohydrates, substituted nucleotides, substituted polynucleotides, and combinations thereof. As used herein, the term "water-soluble portion" or "water-soluble group" (WSG or W) refers by itself or as part of another group to any hydrophilic group that is fully solvated in an aqueous environment (e.g., such as, under physiological conditions) and is capable of increasing the water solubility of the molecule to which it is attached. Any convenient WSG may be included in the dyes described herein to provide increased water solubility. The water-soluble portion may increase the solubility of the compound in a predominantly aqueous solution compared to a control compound lacking a water-soluble portion. The water-soluble portion may be any convenient hydrophilic portion that is fully solvated in an aqueous environment. In some cases, the water-soluble portion may be capable of imparting a solubility in water (e.g., an aqueous buffer) of >1 mg / mL, >2 mg / mL, >3 mg / mL, >4 mg / mL, >5 mg / mL, >6 mg / mL, >7 mg / mL, >8 mg / mL, >9 mg / mL, or >10 mg / mL. In some cases, the water-soluble portion can be capable of conferring a solubility in water of >10 mg / mL, >20 mg / mL, >30 mg / mL, >40 mg / mL, >50 mg / mL, >60 mg / mL, >70 mg / mL, >80 mg / mL, >90 mg / mL, or >100 mg / mL. The increase in water solubility of a molecule can vary depending on the part connected. In some cases, the increase in water solubility (compared to the solubility of the molecule without the connected part) is 2 times or more, 5 times or more, 10 times or more, 25 times or more, 50 times or more, or 100 times or more. In some cases, the water-soluble part is charged, such as a hydrophilic part with a positive charge or a negative charge. In some cases, the water-soluble part is a neutral hydrophilic part. In some cases, the water-soluble part is branched (for example, as described herein). In some cases, the water-soluble part is linear. The water-soluble part includes but is not limited to those taught in U.S. Patent Publication No. 2022 / 0348770, which is incorporated herein by reference in its entirety. A "water-soluble compound" may exhibit a solubility in water (e.g., an aqueous buffer) of >1 mg / mL, >2 mg / mL, >3 mg / mL, >4 mg / mL, >5 mg / mL, >6 mg / mL, >7 mg / mL, >8 mg / mL, >9 mg / mL, or >10 mg / mL at ambient room temperature. In some cases, a water-soluble compound may exhibit a solubility in water of >10 mg / mL, >20 mg / mL, >30 mg / mL, >40 mg / mL, >50 mg / mL, >60 mg / mL, >70 mg / mL, >80 mg / mL, >90 mg / mL, and / or >100 mg / mL at ambient room temperature. Any convenient WSG may be included in the dyes described herein to provide enhanced water solubility. The WSG may be, but is not limited to, carboxylates, phosphonates, phosphates, sulfonates, sulfates, sulfinates, sulfonium, esters, polyethylene glycol (PEG) and modified PEGs, linear PEG groups, branched PEG groups, hydroxyls, amines, amino acids, ammonium, guanidines, Salt, pyridine Polyamines and sulfoniums, polyols, linear or cyclic sugars, primary, secondary, tertiary or quaternary amines and polyamines, phosphonate groups, phosphinate groups, ascorbate groups, glycols including polyethers, zwitterionic derivatives, peptide sequences, nucleotides (DNA and RNA), peptoids, carbohydrates, Oxazoline, polyol, dendron, dendritic polyglycerol, cellulose, chitosan, -COOM', -SO3M', -PO3M', -NR 3 + 、Y'、(CH2CH2O) pR, and mixtures thereof, wherein Y' can be any halogen, sulfate, sulfonate or oxyanion, p can be from 1 to 500, each R can independently be H or an alkyl group (e.g., methyl) and M' can be a cationic counterion or hydrogen, -(CH2CH2O) yy CH2CH2XR yy 、-(CH2CH2O) yy CH2CH2X-、-X(CH2CH2O) yy CH2CH2-, ethylene glycol and polyethylene glycol, wherein yy is selected from 1 to 1000, and X is selected from O, S and NR ZZ , and R ZZ and R YY Independently selected from H and C 1-3 Alkyl, and combinations or derivatives thereof. In some cases, WSG includes, but is not limited to, PEG, modified PEG, peptide sequences, peptoids, carbohydrates, Oxazoline, polyol, dendrimer, dendritic polyglycerol, cellulose, chitosan or a derivative thereof. WSG may be unsubstituted or substituted. In some cases, WSG can be a hydrophilic polymer. For example, hydrophilic polymers that can be used for WSG include, but are not limited to, polyalkylene oxide-based polymers containing the formula -(CH2-CH2-O) n -or-(O-CH2-CH2) n -ethylene oxide repeating units, such as, for example, PEG, polyamide alkylene oxide or its derivatives. Other examples of target polymers include polyamides with a molecular weight greater than 1,000 Daltons, whose formula is -[C(O)-XC(O)-NH-Y-NH] n -or-[NH-Y-NH-C(O)-XC(O)] n -, wherein X and Y are divalent groups which may be the same or different and may be branched or linear, and n is a discrete integer from 2 to 100 (e.g., 2 to 50), and wherein one or both of X and Y comprise biocompatible, substantially non-antigenic, water-soluble repeating units which may be linear or branched. The number of such water-soluble repeating units may vary significantly, wherein the number of such units is 2 to 500, 2 to 400, 2 to 300, 2 to 200, 2 to 100, 6 to 100, e.g., 2 to 50 or 6 to 50. An example of one embodiment is an example where one or both of X and Y are selected from: -((CH2) n1 -(CH2-CH2-O) n2 -(CH2)- or -((CH2) n1 -(O-CH2-CH2) n2 -(CH2) n1-), wherein n1 is 1 to 6, 1 to 5, 1 to 4 or 1 to 3, and wherein n2 is 2 to 50, 2 to 25, 2 to 15, 2 to 10, 2 to 8 or 2 to 5. In some cases, the water-soluble polymer is a group of 1 to 50 monomer units, such as 1 to 40, 1 to 30, 1 to 20, 2 to 24, 2 to 20, 2 to 10 or 2 to 6 monomer units. Another example of an embodiment is an example where X is -(CH2-CH2)-, and where Y is -(CH2-(CH2-CH2-O)3-CH2-CH2-CH2)- or -(CH2-CH2-CH2-(O-CH2-CH2)3-CH2)-. In some cases, any of the formulae described herein may be substituted with a water-soluble moiety that is a dendrimer, as known in the art. In some cases, the hydrophilic polymer can be, for example, PEG, a peptide sequence, a peptoid, a carbohydrate, Oxazoline, polyol, dendrimer, dendrimer polyglycerol, cellulose, chitosan or derivatives thereof. In some cases, WSG is (CH2) x (OCH2CH2) y OCH3, wherein each x is independently an integer from 0 to 20 and each y is independently an integer from 0 to 50. In some cases, the water soluble polymer is a PEG group or modified PEG polymer of 6 to 24 monomer units, such as 10 to 30, 10 to 24, 10 to 20, 12 to 24, 12 to 20, 12 to 16, or 16 to 20 monomer units. In some cases, WSG includes a nonionic polymer (e.g., a PEG polymer) substituted at the terminus with an ionic group (e.g., a sulfonate). In some embodiments of the formula, WSG includes a group selected from (CH2) x (OCH2CH2) y OCH3, wherein each x is independently an integer from 0 to 20, each y is independently an integer from 0 to 50; and the benzyl group is optionally substituted by one or more halogen, hydroxyl, C1-C 12 Alkoxy or (OCH2CH2) z OCH3 substituted, wherein each z is independently an integer from 0 to 50. In some cases, WSG is (CH2)3(OCH2CH2) 11 In some embodiments, one or more of the substituents is replaced by at least one selected from (CH2) x (OCH2CH2) yA benzyl group substituted with a WSG group (e.g., one or two WSG groups) of OCH3, wherein each x is independently an integer from 0 to 20, and each y is independently an integer from 0 to 50. It should be understood that hydroxyl-terminated polymer chains (e.g., PEG chains) rather than methoxy-terminated polymer chains (e.g., PEG chains) can be used in any water-soluble portion. The term modified polymer, such as modified PEG, refers to a water-soluble polymer that is modified or derivatized at one or both ends, for example, to include a terminal substituent (e.g., a terminal alkyl, substituted alkyl, alkoxy, or substituted alkoxy, etc.) and / or a terminal connecting functional group (e.g., an amino or carboxylic acid group suitable for connecting via amide bond formation) suitable for connecting the polymer to a molecule of interest (e.g., connecting to a light-harvesting chromophore via a branching group). The subject water-soluble polymer may be suitable for including any convenient connecting group. It should be understood that in some cases, depending on the preparation and / or purification methods of the polymer starting material, the water-soluble polymer may include some dispersity relative to the polymer length. In some cases, the water-soluble polymer is monodisperse. The water-soluble polymer may include one or more spacers or linkers. Some examples of spacers or linkers include linear or branched moieties comprising one or more repeating units employed in the water-soluble polymer, diamino and or diacid units, natural or non-natural amino acids or derivatives thereof, and aliphatic moieties including alkyl, aryl, heteroalkyl, heteroaryl, alkoxy, etc., which may contain, for example, up to 18 carbon atoms or even additional polymer chains. The water-soluble polymer portion, or one or more spacers or linkers of the polymer portion (when present), may include biostable or biodegradable polymer chains or units. For example, polymers with repeated connections have varying degrees of stability under physiological conditions, depending on the instability of the bond. Based on the known hydrolysis rates of low molecular weight analogs, polymers with such bonds can be classified by their relative hydrolysis rates under physiological conditions, for example, from less stable to more stable: for example, polyurethane (-NH-C(O)-O-)> polyorthoester (-OC((OR)(R'))-O-)> polyamide (-C(O)-NH-). Similarly, the connection system connecting the water-soluble polymer to the target molecule can be biostable or biodegradable, for example, from less stable to more stable: carbonate (-OC(O)-O-)> ester (-C(O)-O-)> carbamate (-NH-C(O)-O-)> orthoester (-OC((OR)(R'))-O-)> amide (-C(O)-NH-). In general, it may be desirable to avoid the use of sulfated polysaccharides based on the instability of the sulfate groups. In addition, it may be less desirable to use polycarbonates and polyesters. These bonds are provided by way of example only and are not intended to limit the type of bonds employed in the linkage system or polymer chain of the water-soluble polymers useful in the WSG disclosed herein. In some cases, water soluble moieties include, but are not limited to, hydroxyl, alkoxy, (hetero)aryloxy, (hetero)arylamino, PEG, linked PEG, amide-PEG, sulfonamide-PEG, phosphoamide-PEG, alkylammonium salts, alkyloxyammonium salts, oligoether ammonium salts, carbonyl, acyl, sulfonate, alkyl sulfonate, alkyl carboxylate, alkyl sulfonate, alkoxy sulfonate, oligoether sulfonate, sulfonamido oligoether, sulfonamide, sulfenamide, phosphonamic acid ester, phosphinamide, alkoxysulfonamide PEG, alkyl carboxylate, alkylamide, alkoxy sulfonate, alkyl sulfonate, alkyl sulfonate, In some cases, the subject compound may include multiple water-soluble moieties connected to a single position in the subject compound, for example, via a branched linker (e.g., such as an aralkyl substituent further disubstituted with a water-soluble group). Thus, in some cases, the branched linker group is a substituent of a dye that connects the dye to two or more water-soluble groups. In some cases, multiple water-soluble moieties may be connected to the subject compound via a group having, for example, the following formula: , where X 1 , X 2 is the branch point, L 1 , L 2 , L 3is a linker, m' is an integer of 1, 2 or 3; W 1 It is the water-soluble part. In some cases, one or more water-soluble moieties may be connected to the subject compound via a group comprising a linker according to the present disclosure, for example, as taught in U.S. Published Application No. 2020 / 0190253A1, which is incorporated herein by reference in its entirety. The linker moiety may be connected to the cyanine bridge or heterocyclic aromatic group of the fluorescent compound of the present disclosure. The linker may be cleavable or non-cleavable. One or more water-soluble moieties may also be attached to the subject compound via a group comprising a linker, such as, but not limited to, the following linker formula (VIe): -(L 3 ) m -(X 1 ) m’ -((L 1 ) m” -(W 1 ) s ) t -R 3 (VIe) in: Each optional L 1 and L 3 is an independently selected linker moiety; Optional X 1 is a branch point; W 1 is a water-soluble portion, including but not limited to a water-soluble polymer comprising 2 to 50, 4 to 30, or 6 to 24 monomer units; Each m is independently 0 or 1; each m' is independently 0 or 1; each m" is independently 0 or 1; Each s is independently 1 or 2; each t is independently 0, 1, 2 or 3; and R 3 As defined herein. In some cases, L 1 , L 3 and X does not exist, and W 1 The water-soluble portion is a water-soluble polymer comprising, for example, 2 to 50, 4 to 30, or 6 to 24 monomer units, such as 10 to 30, 10 to 24, 10 to 20, 12 to 24, 12 to 20, 12 to 16, or 16 to 20 monomer units. In some cases, the water-soluble portion can be a linear water-soluble portion. For example, L 1 and X may not exist, L 3 is a linker (e.g., as disclosed herein), and W 1It is the water-soluble part. In some cases, L 1 , L 2 and / or L 3 At least one, at least two or all three of the above can be selected from alkyl or substituted alkyl joints, alkenyl or substituted alkenyl joints, alkynyl or substituted alkynyl joints, alkoxy or substituted alkoxy joints, PEG joints, sulfonamido-alkyl or substituted sulfonamido-alkyl joints, amido-alkyl or substituted amido-alkyl joints and alkyl-amido-alkyl or substituted alkyl-amido-alkyl joints. In some cases, the joint comprises a carbonyl. The joint moiety can be a covalent bond, alkoxy, sulfonamide, disulfonamide, selenoamide, sulfenamide, sultam, disulfenamide, amide, carbonyl, selenamide, phosphamide, phosphinamide, phosphinamide ester or secondary amine. In some cases, L 2 and L 3 Can be linker moieties independently selected from the following: covalent bond, C 1-8 Alkylene, 2 to 8 membered heteroalkylene, and chains of 2 to 200 backbone atoms in length, wherein the chain contains linear chains, branched chains, and / or cyclic portions. In some cases, L 1 It may be a sulfonamide, sulfenamide, disulfonamide, disulfenamide, sultam, amide, secondary amine, phosphamide, phosphinamide, phosphonamidate, selenoamide or selenamide. In some cases, L 3 may be a linker having a backbone length of 20 atoms or less, and W 1 is a water-soluble portion (e.g., as described herein). 3 It can be selected from alkyl or substituted alkyl linkers, alkenyl or substituted alkenyl linkers, alkynyl or substituted alkynyl linkers, acyl or substituted acyl, alkoxy or substituted alkoxy linkers, PEG linkers, sulfonamido-alkyl or substituted sulfonamido-alkyl linkers, amido-alkyl or substituted amido-alkyl linkers, and alkyl-amido-alkyl or substituted alkyl-amido-alkyl linkers. In some cases, L 3 can be a bond. In some cases, L 3 R may be an alkyl or substituted alkyl linker, an alkenyl or substituted alkenyl linker, an alkynyl or substituted alkynyl linker, an alkoxy or substituted alkoxy linker, and X may be an aryl group. In some cases, L 1 and L 3 Each independently selected from C1-C 12 Alkyl or substituted alkyl linker, C1-C 12Alkenyl or substituted alkenyl linker, C1-C 12 Alkynyl or substituted alkynyl linker, C1-C 12 Acyl or substituted acyl linker, C1-C 12 Alkoxy or substituted alkoxy linker, C1-C 12 Amido-alkyl or substituted amido-alkyl linker, C1-C 12 alkyl-amido-alkyl or substituted alkyl-amido-alkyl linkers, sulfonamides, sulfenamides, disulfonamides, disulfenamides, sultams, amides, secondary amines, phosphonamides, phosphinamides, phosphonamidates, selenoamides and selenonamides. In some cases, L 3 contains a carbonyl group or an alkoxy group, and L 1 It is C1-C 12 alkyl or substituted alkyl, sulfonamide, sulfenamide, disulfonamide, disulfenamide, sultam, amide, secondary amine, phosphinamide, phosphinamide, phosphinamate, selenoamide and selenonamide. In some cases, L 3 may be an alkoxy or substituted alkoxy linker, X may be absent, and L 1 It may be a sulfonamide, sulfenamide, disulfonamide, disulfenamide, sultam, amide, secondary amine, phosphamide, phosphinamide, phosphonamidate, selenoamide or selenamide. In some cases, the branch point X 1 is selected from N, CR', C(=O)N, SO2N, a trisubstituted aryl moiety (e.g., 1,3,5-phenyl), a tetrasubstituted aryl moiety (e.g., 1,3,4,5-phenyl), and a trisubstituted heteroaryl. In some cases, the branch point X 1 is a nitrogen atom. In other cases, the branch point X 1 is CR', wherein R' is selected from hydrogen, alkyl, substituted alkyl or -L 3 -W 1 (e.g., as described herein). When referring to polymers used herein, the term "water-soluble" refers to polymers that have a solubility in "water" as used herein of 1 mg / mL or greater, such as 3 mg / mL or greater, 10 mg / mL or greater, 20 mg / mL or greater, 30 mg / mL or greater, 40 mg / mL or greater, 50 mg / mL or greater, 60 mg / mL or greater, 70 mg / mL or greater, 80 mg / mL or greater, 90 mg / mL or greater, 100 mg / mL or greater, or even greater. It should be understood that under certain conditions, water-soluble polymers can form discrete water-solvated nanoparticles in aqueous systems and can resist aggregation. The fluorescent compounds of the present disclosure can be water-soluble. The fluorescent polymers of the present disclosure can be water-soluble. As used herein, the term "cyanine" refers to a substituted or unsubstituted bridging unit that allows delocalization across the molecules or monomers of the invention. In some embodiments, "cyanine" is a substituted or unsubstituted methine or polymethine unit, such as a tri-, penta-, or hepta-methine unit. For example, in some embodiments, "cyanine" refers to substituted and unsubstituted groups, such as the following: In some embodiments, cyanine is In other embodiments, cyanine is In yet other embodiments, cyanine is As used herein, the term "squarylium" refers to a substituted or unsubstituted cyclic group, such as a 4-membered ring. In addition, the molecules and monomers of the present invention include other "squarylium"-like cyclic groups and heterocyclic groups, including substituted or unsubstituted five-membered or six-membered cyclic groups and heterocyclic groups. For example, squarylium and squarylium-like groups include, but are not limited to, substituted or unsubstituted cyclic groups and heterocyclic groups, such as the following: wherein C is a four-membered, five-membered, or six-membered cyclic group. In some embodiments, the cyclic squarylium group is In some embodiments, the cyclic squarylium-like ring group is In some embodiments, the cyclic squarylium-like ring group is In other embodiments, the cyclic squarylium-like ring group is In yet other embodiments, the cyclic squarylium-like ring group is III. DHP-cyanine and DHP-squarylium cyanine monomers The present disclosure provides DHP-cyanine and DHP-squaryl cyanine organic dye monomer compounds, polymers and preparation methods. Both small organic dyes and polymeric dyes can be prepared according to the method provided in the present disclosure. For monomeric dyes, the DHP-cyanine and DHP-squaryl cyanine dyes of the gained show excitation maximum in the range of about 420 to 900nm. Except the specific structures disclosed herein, also include the structural isomers of the disclosed structure. The DHP-cyanine and DHP-squarylium compounds of the present disclosure each comprise at least one 9,10-dihydrophenanthrene (DHP) moiety or a derivative thereof. The DHP-cyanine or DHP-squarylium compound according to the present disclosure may comprise a structure according to formula (I): in: D is aryl, heteroaryl, J is aryl, heteroaryl or and At least one of D or J is independently selected from: Wherein optional Ar and optional Are each independently selected from substituted or unsubstituted benzene, benzene derivatives, monocyclic aryl, polycyclic aryl, monocyclic heteroaryl and polycyclic heteroaryl, wherein at least one of D or J Must be present and contain a DHP structure Or a derivative thereof, said derivative containing an additional aryl or heteroaryl fused at any available Position. In some cases, D is J is And Is Or a derivative thereof, said derivative containing an additional aryl or heteroaryl fused at any available Position, optionally wherein said derivative is In some cases, D is J is In D Is Or a derivative thereof, said derivative containing an additional aryl or heteroaryl fused at any available Position, optionally wherein said derivative is And in J Is substituted or unsubstituted benzene, or a derivative thereof. In some cases, D is J is In D Is substituted or unsubstituted naphthalene, and in J Is Or a derivative thereof, said derivative containing an additional aryl or heteroaryl fused at any available Position, optionally wherein said derivative is Can be fused with the DHP structure Or a derivative thereof at any available Position, for example at the 1,2-, 2,3-, 3,4-, 5,6-, 6,7- and 7,8- positions of the DHP structure or a derivative thereof. Each T can be independently selected from C(R 1 )、N、P、O、S and Si(R 1 ). Each T can be independently C(R 1 ). or N. Each U can be independently selected from NR 10 、O、P and S. Each U can be independently NR 10 . Each V can be independently selected from NR 11 , CR 11 , C(R 11 )2, S, O and Si(R 11 )2. Each V can be independently CR 11 , C(R 11 )2, S or O. Each V can be independently CR 11 or C(R 11 )2. Each V' may be independently selected from SO2, SO, S, NR 11 , CR 11 , C(R 11 )2,O,Si(R 11 )2, >C=O, >Se=O, -CH=CH-, and -N=CH-. Each V' may be independently selected from SO2, SO and S. Each X can be independently CR 1 R 2 or SiR 1 R 2 In some cases, each X may independently be CR 1 R 2 . Each Y can independently be CR 8 R 9 or SiR 8 R 9 In some cases, each Y can independently be CR 8 R 9 . Each R 1 , R 2 , R 8 and R 9 can be independently selected from a water-soluble moiety, a linker moiety, a linked E, hydrogen, hydroxyl, halogen, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, haloalkyl, aryl, heteroaryl, (hetero)aryloxy, (hetero)arylamino, sulfonamide-PEG, phosphoamide-PEG, alkylammonium salts, alkyloxyammonium salts, oligoether ammonium salts, carbonyl, acyl, alkylsulfonate, alkoxysulfonate, oligoethersulfonate, sulfonamidooligoether, sulfonamide, sulfenamide, phosphoamidate, phosphinamide, alkoxysulfonamide PEG, alkoxysulfonate, alkylsulfonate, alkylsulfonate, alkylsulfonate, , or R 1 and R 8 Together they form an unsubstituted or substituted cycloalkyl, cycloalkenyl, heterocycloalkyl, heterocycloalkenyl, cycloalkoxy, aryl or heteroaryl group having 3 to 9 ring members. Each R 3The moiety may be independently selected from H, alkyl, alkene, alkyne, cycloalkyl, haloalkyl, alkoxy, (hetero)aryloxy, aryl, (hetero)arylamino, a water soluble moiety, a chromophore, a functional moiety, a binding partner and a PEG group. Each Q can independently be a bond, NH, NR 4 、C1-C 12 Alkylene, CHR 4 and CH2CH2, CHR 4 , O, NR 4 or NH; each Z is independently CH2, CHR 4 , O, NR 4 or NH. Each W 1 May independently be a water-soluble portion. Each L 1 , L 2 and L 3 Each E is independently a selected linker moiety; each E is independently selected from a chromophore, a functional moiety, a substrate, and a binding partner. Each R 4 The groups can be independently selected from H, alkyl, PEG, a water soluble moiety, a linker moiety, a chromophore, a carboxylic acid amide, an amine, a carbamate, a carboxylic acid, a carboxylic acid ester, a maleimide, an activated ester, an N-hydroxysuccinimide group, a hydrazine, a hydrazone, an azide, an alkyne, an aldehyde, and a thiol, or a protected group thereof. Each R 7 Can be independently selected from H, hydroxyl, C1-C 12 Alkyl, C2-C 12 Olefins, C2-C 12 Alkynes, C3-C 12 Cycloalkyl, C1-C 12 Halogenated alkyl, C1-C 12 Alkoxy, C2-C 18 (Hetero)aryloxy, C2-C 18 (Hetero)arylamino, C2-C 12 Carboxylic acid, C2-C 12 Carboxylic acid esters and C1-C 12 alkoxy, water soluble moiety, PEG moiety, functional group, chemoselective functional group, conjugated tag, attached conjugated tag, linker, sulfonic acid, sulfonate, C1-C 12 Alkyl sulfonate / ester, sulfonamide. In some cases, each R7 can be independently selected from H, hydroxyl, C1-C 12 Alkyl, C2-C 12 Olefins, C2-C 12 Alkynes, C3-C 12 Cycloalkyl, C1-C 12Halogenated alkyl, C1-C 12 Alkoxy, C2-C 18 (Hetero)aryloxy, C2-C 18 (Hetero)arylamino, C2-C 12 Carboxylic acid, C2-C 12 Carboxylic acid esters and C1-C 12 In some cases, each R 7 can be independently selected from a functional group, a chemoselective functional group, a conjugated tag, a linked conjugated tag, a linker, a sulfonic acid, a sulfonate, a C1-C 12 Alkyl sulfonates and sulfonamides. Each R 10 Can be independently selected from hydrogen, a linker moiety, a linked reactive group, a linked ionic group, a linked chromophore, a linked water-soluble moiety, a water-soluble moiety, a chromophore, a binding partner, a linked binding partner. Each R 11 Can be independently selected from hydrogen, a linker moiety, a linked reactive group, a linked ionic group, a linked chromophore, a linked water-soluble moiety, a water-soluble moiety, a chromophore, a binding partner, and a linked binding partner. Each R 12 and R 13 can be independently selected from hydrogen, C1-C6 alkyl, C1-C6 alkyl, C3-C 10 cycloalkyl, substituted or unsubstituted aryl, and substituted or unsubstituted heteroaryl; or R 12 and R 13 Together they form an unsubstituted or substituted unsaturated cycloalkyl having 3 to 8 ring members or a substituted or unsubstituted heterocycloalkyl having 3 to 8 ring members, which is optionally substituted with O. Each K may independently be a covalent bond, O, S, P or CH2. Each f may independently be an integer from 0 to 50, 1 to 40, 2 to 30, 3 to 25, or 4 to 20. Each m can independently be 0 or 1. Each n can independently be 0, 1, 2, 3 or 4. Each s can independently be 1 or 2. Each t can independently be 0, 1, 2 or 3. In some instances, R 1 , R 2 , R 3 , R 4 , R 8 , R 9 , R 10 and R 11 At least one of the comprises a water-soluble portion or a linked water-soluble portion. Contained in any available derivatives of additional aryl or heteroaryl fused at optionally may contain where each V' is independently selected from SO2, SO, S, NR 11 , CR 11 , C(R 11 )2, O, Si(R 11 )2, >C=O, >Se=O, -CH-CH-, or -N=CH-, and T, V, X, Y, and m are as defined above. The DHP-cyanine or DHP-squarylium compounds according to the present disclosure may contain a structure according to formula (II): wherein: each is independently selected from substituted or unsubstituted benzene, benzene derivatives, monocyclic aryl, polycyclic aryl, monocyclic heteroaryl, and polycyclic heteroaryl, wherein at least one contains or its derivatives, wherein the derivatives optionally contain additional aryl or heteroaryl fused at any available position. In some cases, the derivatives of are 11 where each V' is independently SO2, SO, S, NR 11 , CR 11 , C(R 11 )2, O, Si(R each T is independently selected from C, C(R 1 ), N, P, O, S, and Si(R 1 ); each U is independently selected from NR 10 , O, P, Se, Te, and S; each V is independently selected from NR 11 , CR 11 , C(R 11 )2, S, SO2, O, Se, Te, and Si(R 11 )2; each X is independently CR 1 R 2 or SiR 1 R 2 ; each Y is independently CR 8 R 9 or SiR 8 R 9;as well as Each m is independently 0 or 1. In some cases, each Independently wherein each T is independently selected from C and C(R 1 ); Each U is independently selected from NR 10 , O, P, Se, Te and S; Each V is independently selected from NR 11 , CR 11 , C(R 11 )2, S, SO2, O, Se, Te and Si(R 11 )2; Each X is independently CR 1 R 2 or SiR 1 R 2 ; Each Y is independently CR 8 R 9 or SiR 8 R 9 ;as well as Each m is independently 0 or 1. In some cases, each Independently wherein each T is independently selected from C and C(R 1 ); Each U is independently NR 10 ; Each V is independently selected from CR 11 and C(R 11 )2; Each X is independently CR 1 R 2 ; Each Y is independently CR 8 R 9 ;as well as Each m is independently 0 or 1. In some cases, a The group is substituted or unsubstituted benzene, a benzene derivative, or substituted or unsubstituted naphthalene, or a naphthalene derivative, and the other Groups include or a derivative thereof, wherein the derivative is optionally contained in any available The derivative is optionally wherein each T is independently selected from C and C(R 1 ); Each U is independently NR 10 ; Each V is independently selected from CR 11 and C(R 11 )2; Each V' is independently selected from SO2, SO and S; Each X is independently CR 1 R 2 ; Each Y is independently CR 8 R 9 ;as well as Each m is independently 0 or 1. In some cases, V is CR 11 or C(R 11 )2. Each R 1 , R 2 , R 8 and R 9 can be independently selected from a water soluble moiety, a linked water soluble moiety, a linker moiety, a linked E, a reactive group, a linked reactive group, a binding partner, a linked binding partner, a functional group, hydrogen, hydroxyl, halogen, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, haloalkyl, aryl, heteroaryl, (hetero)aryloxy, (hetero)arylamino, sulfonamide-PEG, phosphoamide-PEG, alkylammonium salt, alkyloxyammonium salt, oligoether ammonium salt, carbonyl, acyl, alkylsulfonate, alkoxysulfonate, oligoethersulfonate, sulfonamidooligoether, sulfonamide, sulfenamide, phosphoamidate, phosphinamide, alkoxysulfonamide PEG, alkoxysulfonate, alkylsulfonate, alkylsulfonate, alkylsulfonate, In some cases, each R 1 , R 2 , R 8 and R 9 R is independently selected from a water soluble moiety, a linked water soluble moiety, a linker moiety, a linked E, a reactive group, a linked reactive group, a binding partner, a linked binding partner, a functional group, hydrogen, hydroxyl, halogen, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, and haloalkyl. In some cases, each R 1 , R 2 , R 8 and R 9 Can be independently selected from watrsulfonamide-PEG, phosphonamide-PEG, alkylammonium salts, alkyloxyammonium salts, oligoether ammonium salts, carbonyl, acyl, alkyl sulfonate, alkoxy sulfonate, oligoether sulfonate, sulfonamido oligoether, sulfonamide, sulfenamide, phosphonamidate, phosphinamide, alkoxysulfonamide PEG, alkoxy sulfonate, alkyl sulfonate, alkyl sulfonate, In some cases, each R 1 , R 2 , R 8 and R 9 R can be independently selected from a water soluble moiety, a linked water soluble moiety, a linker moiety, a linked E, a reactive group, a linked reactive group, a binding partner, a linked binding partner, a functional group. In some cases, each R 1 , R 2 , R 8 and R 9 The radicals may be independently selected from hydrogen, hydroxy, halogen, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl and haloalkyl. In some cases, R 1 and R 8 Together they form an unsubstituted or substituted cycloalkyl, cycloalkenyl, heterocycloalkyl, heterocycloalkenyl, cycloalkoxy, aryl or heteroaryl group having 3 to 9 ring members. In some cases, each R 3 R is independently selected from H, alkyl, alkene, alkyne, cycloalkyl, haloalkyl, alkoxy, (hetero)aryloxy, aryl, (hetero)arylamino, a water soluble moiety, a chromophore, a functional moiety, a binding partner, and a PEG group. 3 independently selected from a water-soluble moiety, a chromophore, a functional moiety, a binding partner, and a PEG group (e.g., (PEG) f -OMe and (PEG) f -OEt). In some cases, each Q is independently a bond, NH, NR 4 、C1-C 12 Alkylene, CHR 4 or CH2. In some cases, each Q is independently a bond, NH or NR 4 . In some cases, each Z is independently CH2, CHR 4 , O, NR 4 In some cases, Z is O. In some cases, each W 1 Independently is the water-soluble portion. In some cases, L 1 , L 2 and L 3 are each independently selected linker moieties. In some cases, each E is independently selected from a chromophore, a functional moiety, a conjugated tag, a substrate, and a binding partner. In some cases, each R 4Independently selected from H, alkyl, PEG, a water soluble moiety, a linker moiety, a chromophore, a linked chromophore, a functional group, a conjugated tag, a carboxylic acid amide, an amine, a carbamate, a carboxylic acid, a carboxylate, a maleimide, an activated ester, an N-hydroxysuccinimidyl group, a hydrazine, a hydrazone, an azide, an alkyne, a cycloalkyne, an alkene, a cycloalkene, a tetrazine, an aldehyde, and a thiol, or a protected group thereof. In some cases, each R 7 Independently selected from H, hydroxyl, C1-C 12 Alkyl, C2-C 12 Olefins, C2-C 12 Alkynes, C3-C 12 Cycloalkyl, C1-C 12 Halogenated alkyl, C1-C 12 Alkoxy, C2-C 18 (Hetero)aryloxy, C2-C 18 (Hetero)arylamino, C2-C 12 Carboxylic acid, C2-C 12 Carboxylic acid esters and C1-C 12 Alkoxy. In some cases, each R 10 Independently selected from hydrogen, a linker moiety, a linked reactive group, a linked ionic group, a linked chromophore, a linked water-soluble moiety, a water-soluble moiety, a chromophore, a binding partner, a linked binding partner. In some cases, each R 11 Independently selected from hydrogen, a linker moiety, a linked reactive group, a linked ionic group, a linked chromophore, a linked water soluble moiety, a chromophore, a binding partner, and a linked binding partner. In some cases, each R 12 , R 13 and R 14 independently selected from hydrogen, halogen, substituted or unsubstituted C1-C6 alkyl, substituted or unsubstituted C1-C6 heteroalkyl, substituted or unsubstituted C1-C6 alkene, substituted or unsubstituted C3-C 10 cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl and substituted or unsubstituted heteroaryl, CO2R 1 ,CONR 1 R 2 、-O-CH2CH2-PEG-R 7 、-S-CH2CH2-PEG-R 7 、-N-CH2CH2-PEG-R 7 , O-aryl, S-aryl, N-aryl, -O-alkyl, S-alkyl, N-alkyl, wherein each alkyl or aryl group may be optionally replaced by one or more R7 , PEG, PEG-R 7 or a linking group, wherein each R 12 , R 13 and R 14 Independently by R 7 Group substitution; or R 10 , R 11 , R 12 , R 13 and R 14 At least two of the 10 , R 11 , R 12 , R 13 and R 14 Two, three or four of the above) together form an unsubstituted or substituted unsaturated or partially unsaturated C3-C 10 Cycloalkyl, unsubstituted or substituted, unsaturated or partially unsaturated C3-C 10 heterocycloalkyl, which is optionally substituted by O, unsubstituted or substituted unsaturated or partially unsaturated cycloalkyl having 3 to 8 ring members, or substituted or unsubstituted heterocycloalkyl having 3 to 8 ring members, which is optionally substituted by O; In some cases, each K is independently a covalent bond, O, S, P, NR 1 , Se, Te, CR 1 R 2 or CH2. In some cases, each f is independently an integer from 0 to 50. In some cases, each m is independently 0 or 1. In some cases, each n is independently 0, 1, 2, 3, or 4. In some cases, each s is independently 1 or 2. In some cases, each t is independently 0, 1, 2, or 3. In some examples, the DHP-cyanine and DHP-squarylium compounds of the present disclosure can be selected from formula (IIa), (IIb), (IIc), (IId), (IIe), (IIf), (IIg), (IIh), (IIi), (IIj), (Ik), (IIl), (IIm), (IIn), (IIo), (IIp), (IIq), (IIr), (IIs), (IIt), (IIu), (IIv), (IIw), (IIx), (IIy), or (IIz): In some examples, the DHP-cyanine and DHP-squarylium cyanine compounds of the present disclosure can be selected from formula (IIIa), (IIIb), (IIIc), (IIId), (IIIe), (IIIf), (IIIg), (IIh), (IIIi), (IIIj), (IIIk), (IIIl), (IIm), (IIIn), (IHo), (IIIp), (IIq), (IIIr), (IIIs), (IIIt), (IIIu), (IIIv), (IIIw), (IIIx), (IIIy), (IIIz), (IIIaa), (IIIbb), (IIIcc), (IIIdd), or (IIIee): In some instances, D and J The group may contain the same or different In some examples, D and J are Groups contain the same In some examples, D and J are The group contains different group or its derivatives. In some embodiments, D is Among them, D yes or its derivatives; and J is aryl, heteroaryl or Among them, J It is an unsubstituted or substituted benzene, a benzene derivative containing a fused monocyclic aromatic group, a polycyclic aromatic group, a monocyclic heteroaromatic group or a polycyclic heteroaromatic group. In some instances, J is Among them, J is unsubstituted or substituted benzene or unsubstituted or substituted naphthalene; U is N; and V is N, O or S. In some embodiments, J is unsubstituted or substituted quinoline, benzo Azoles, benzothiazoles, benzimidazoles, indoles, benzindole, pyridine Benzopyran Thiopyran Fluorene, 9H-fluorene, phenanthrene, dihydrophenanthrene, 9,10-dihydrophenanthrene, 6,8-dihydro-5H-naphtho[2,1-f]indole, 4,5-dihydro-3H-naphtho[2,1-e]indole, 6,7-dihydro-3H-naphtho[2,1-g]indole, 5,6-dihydrophenanthro[3,2-d]thiazole, 4,5-dihydrophenanthro[2,1-d]thiazole, 6,7-dihydrophenanthro[4,3-d]thiazole, 5,6-dihydrophenanthro[3,2-d] oxadiazole, 4,5-dihydrophenanthro[2,1-d] oxadiazole, 6,7-dihydrophenanthro[4,3-d] oxadiazole, 5,6-dihydronaphtho[2,1-g]quinoline, 7,8-dihydronaphtho[2,1-h]quinoline, 5,6-dihydronaphtho[2,1-f]quinoline, 5,6-dihydro-8λ2-phenanthro[3,2-d]imidazole, 4,5-dihydro-3λ2-phenanthro[2,1-d]imidazole, 6,7-dihydro-3λ2-phenanthro[4,3-d]imidazole, 5,6-dihydronaphtho[1,2-g]quinoxaline, 5,6-dihydronaphtho[2,1-f]quinoxaline, 7,8-dihydronaphtho[1,2-f]quinoxaline, phenyl, 1H-benzo[e]indole-3- and 1H-benz[e]indole. In some embodiments, D is aryl, heteroaryl or Among them, D is substituted or unsubstituted benzene, benzene derivatives, monocyclic aromatic groups, polycyclic aromatic groups, monocyclic heteroaromatic groups, and polycyclic heteroaromatic groups; and J is Among them, J yes or its derivatives. In some embodiments, D is Among them, D is unsubstituted or substituted benzene or unsubstituted or substituted naphthalene; U is N; and V is N, O or S. In some embodiments, D is selected from unsubstituted or substituted quinoline, benzo Azoles, benzothiazoles, benzimidazoles, indoles, benzindole, pyridine Benzopyran Thiopyran Fluorene, 9H-fluorene, phenanthrene, dihydrophenanthrene, 9,10-dihydrophenanthrene, 6,8-dihydro-5H-naphtho[2,1-f]indole, 4,5-dihydro-3H-naphtho[2,1-e]indole, 6,7-dihydro-3H-naphtho[2,1-g]indole, 5,6-dihydrophenanthro[3,2-d]thiazole, 4,5-dihydrophenanthro[2,1-d]thiazole, 6,7-dihydrophenanthro[4,3-d]thiazole, 5,6-dihydrophenanthro[3,2-d] oxadiazole, 4,5-dihydrophenanthro[2,1-d] oxadiazole, 6,7-dihydrophenanthro[4,3-d] oxadiazole, 5,6-dihydronaphtho[2,1-g]quinoline, 7,8-dihydronaphtho[2,1-h]quinoline, 5,6-dihydronaphtho[2,1-f]quinoline, 5,6-dihydro-8λ2-phenanthro[3,2-d]imidazole, 4,5-dihydro-3λ2-phenanthro[2,1-d]imidazole, 6,7-dihydro-3λ2-phenanthro[4,3-d]imidazole, 5,6-dihydronaphtho[1,2-g]quinoxaline, 5,6-dihydronaphtho[2,1-f]quinoxaline, 7,8-dihydronaphtho[1,2-f]quinoxaline, phenyl, 3,5,6,8-tetrahydrophenanthro[2,3-e:7,6-e']diindole, 5,6-dihydropentaphenanthro[3,4-d:10,9-d']bis( azole), 5,6-dihydropentaphenanthro[3,4-d:10,9-d']bis(thiazole), 6,7-dihydrophenanthro[2,3-f:7,6-f']diquinoline, 5,6-dihydropentaphenanthro[3,2,1-cd:10,11,12-c'd']diindole, 2,6,7,11-tetrahydrobenzo[1,2-g:4,3-g']dichromene, or 2,6,7,11-tetrahydrobenzo[1,2-g:4,3-g']bis(thiochromene). In some embodiments, D is Or J is in: At least one yes A group or a derivative thereof, wherein the derivative Any available Location and Condensed. In some embodiments, D is and The group or its derivatives Any available Location and Condensed. In some examples, D can be selected from: In some embodiments, D is Among them, D yes A group or a derivative thereof, wherein the derivative Any available Location and Condensed. In some instances, J is in The group is A group or a derivative thereof, wherein the derivative Any available Location and Condensed. In some examples, J is selected from: In some instances, at least one The group or its derivatives are selected from: Each R 5 independently selected from halogen, hydroxyl, C1-C 12 Alkyl, C2-C 12 Olefins, C2-C 12 Alkynes, C3-C 12 Cycloalkyl, C1-C 12 Halogenated alkyl, C1-C 12 Alkoxy, C2-C 18 (Hetero)aryl, C2-C 18 (Hetero)aryloxy, C2-C 18 (Hetero)arylamino, carboxylic acid, carboxylate, (CH2) x’ (OCH2-CH2) y’ OCH3 and (CH2) x’ (OCH2-CH2) y’ OCF3, wherein each x' is independently an integer from 0 to 20, and each y' is independently an integer from 0 to 50. In some examples, D is selected from: In some examples, J is selected from: In some examples, each R 10 and each R 11independently selected from a water soluble moiety, a linker moiety, a linked water soluble moiety, hydrogen, an alkyl, an alkene, an alkyne, a cycloalkyl, a haloalkyl, a (hetero)aryloxy, an aryl, a heteroaryl, a (hetero)arylamino, a sulfonamide-PEG, a phosphoamide-PEG, an alkylammonium salt, an alkyloxyammonium salt, an oligoether ammonium salt, an alkylsulfonate, an alkoxysulfonate, an oligoethersulfonate, a sulfonamidooligoether, a sulfonamide, a sulfenamide, a phosphoamidoester, a phosphinamide, an alkoxysulfonamide PEG, an alkoxysulfonate, an alkylsulfonate, an alkylsulfonate, a In some cases, each R 10 and each R 11 independently comprising a moiety selected from the group consisting of sulfonamide-PEG, phosphonamide-PEG, alkylammonium salts, alkyloxyammonium salts, oligoether ammonium salts, alkyl sulfonates, alkoxy sulfonates, oligoether sulfonates, sulfonamido oligoethers, sulfonamides, sulfenamides, phosphonamidates, phosphinamides, alkoxysulfonamide PEGs, alkoxy sulfonates, alkyl sulfonates, alkyl sulfonates, In some cases, each R 10 and each R 11 independently comprising a moiety selected from the group consisting of sulfonamide-PEG, sulfonamido oligoether, sulfonamide, sulfenamide, alkoxysulfonamide PEG, The DHP-cyanine and DHP-squarylium compounds of the present disclosure may include any of the following compounds: The DHP-cyanine and DHP-squarylium compounds of the present disclosure may be water-soluble. Also provided are methods of preparing the DHP-cyanine and DHP-squarylium compounds of the present disclosure. Figure 1 An exemplary synthetic scheme 1 is shown with selected steps for the synthesis of DHP-cyanines and DHP-squarylium cyanines from modified dihydrophenanthrene (DHP) cores. DHP-indole (Va), DHP-thiazole (Vb), DHP- The oxazole (Vc) and DHP-quinoline (Vd) intermediates can be prepared from the DHP core molecule by treatment with appropriate aldehyde diphenylamine hydrochloride or squaric acid to give DHP-cyanine compounds of formula (VI) (where n=0, 1, 2 or 3) or DHP-squarylium compounds of formula (VII), respectively. Figure 2 An exemplary synthesis scheme 2 is shown for modifying dihydrophenanthrene and subsequently synthesizing a DHP-Cy5 type monomeric dye. Commercially available 3-bromophenanthrene-9,10-dione can be used. 6 Reduction with sodium borohydride NaBH4 in water-ethanol provides the 3-bromo-9,10-dihydrophenanthrene-9,10-diol intermediate 7 In the presence of sodium hydride NaH, DHP-diol 7 Treatment with, for example, an alkyl iodide provides 3-bromo-DHP-OR 1 Intermediates 8 In the presence of sodium tert-butoxide, Pd(OAc)2, (±)-BINAP, 8 Treatment with benzophenone hydrazone in toluene provides the DHP-hydrazine intermediate 9 Under acidic conditions, 9 Treatment with methyl isopropyl ketone provided the DHP-indole intermediate 10 .Will 10 Treatment with 1,3-propane sultone affords the 3-sulfopropyl-naphthoindole intermediate 11 In the presence of acetic anhydride, sodium acetate and DIPEA, 11 Treatment with an appropriate aldehyde diphenylamine hydrochloride (e.g., glutaric acid aldehyde diphenylamine hydrochloride) in CH3CN / CH2Cl2 affords DHP-cyanine dyes of structure (VIII) according to the present disclosure. This scheme and its variations can be used to prepare compounds 1 to 5 shown in Table 1. Figure 5 Shown is a synthetic scheme 5 for the preparation of asymmetric DHP-squarylium monomer dyes of formula (X) from a dihydrophenanthrene-type core and a semisquarylium. Figure 5 Also shown is synthetic Scheme 6 for the preparation of symmetrical DHP-squarylium cyanine monomeric dyes of formula (XI) from a dihydrophenanthrene-type core and squaric acid. Table 1 shows exemplary symmetrical and asymmetrical DHP-cyanine compounds 1 to 5 of the present disclosure and their fluorescence properties, including maximum excitation wavelength (λex) and emission wavelength (λem). Table 1. Exemplary DHP-cyanine dyes with fluorescent properties IV. DHP-cyanine dyes and DHP-squarylium cyanine polymers The present disclosure provides DHP-cyanine polymers and DHP-squaryl cyanine polymers and methods of preparation. DHP-cyanine and DHP-squaryl cyanine polymeric dyes can exhibit excitation λmax in the range of 420 to 900 nm. Dihydrophenanthrene with substituted bromine at positions 3 and 6 will produce dyes different from those prepared using previously synthesized monomer A, wherein the bromine atoms are substituted at positions 2 and 7. Thus, several options for preparing different dyes are available from this technology. The polymeric dyes provided in the present disclosure comprise monomers having a structure according to formula (IV) or (V): Where each of (IV) or (V) It is the point of attachment to the polymer dye backbone. Each D 1 and D 2 are independently selected from aryl, heteroaryl and Each of these are independently selected from substituted or unsubstituted benzene, benzene derivatives, monocyclic aromatic groups, polycyclic aromatic groups, monocyclic heteroaromatic groups and polycyclic heteroaromatic groups, wherein D 1 or D 2 At least one of in yes or its derivatives, said derivatives being included in any available fused to another aryl or heteroaryl group, and wherein The group or its derivatives Any available Location and Condensed. Each T,U,V,X,Y,R 1 ,R 2 ,R 3 , R 4 , R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , R 13 ,Q,Z,W1,L 1 , L 2 , L 3 , E, k, f, n, m, n, s, t are as described above. Included in any available Another aryl or heteroaryl group fused to The derivatives may optionally contain Where V' is SO2, SO, S, NR 11 , CR 11 , C(R 11 )2、O、Si(R 11 )2, >C=O, >Se=O, -CH=CH- or -N=CH-, and T, V, X, Y and m are as defined above. In some examples, the monomer of formula (IV) or (V) comprises a structure selected from formula (A1), (A2), (A3), (A4), (A5), (A6), (A7), (A8), (A9), (A10), (A11), (A12), (A13), (A14), (A15), (A16), (A17), (A18), and (A19): In some examples, the present disclosure provides DHP-cyanine or DHP-squarylium cyanine polymer dyes according to formula (VI) or (VII): in: Each D 1 and D 2 are independently selected from aryl, heteroaryl and Each of these are independently selected from substituted or unsubstituted benzene, benzene derivatives, monocyclic aromatic groups, polycyclic aromatic groups, monocyclic heteroaromatic groups and polycyclic heteroaromatic groups, wherein D 1 or D 2 At least one of in yes or its derivatives, said derivatives being included in any available fused to another aryl or heteroaryl group, and wherein The group or its derivatives Any available Location and condense; Each J is independently aryl, heteroaryl, in is substituted or unsubstituted benzene, benzene derivatives, monocyclic aromatic groups, polycyclic aromatic groups, monocyclic heteroaromatic groups, and polycyclic heteroaromatic groups, and p is an integer from 2 to 1,000, 5 to 500, or 10 to 100. In some embodiments, at least one J group is in The group is group or its derivatives, said derivatives being included in any available fused to another aryl or heteroaryl group, and wherein The group or its derivatives Any available Location and Condensed. In some examples, the present disclosure provides DHP-cyanine or DHP-squarylium cyanine polymer dyes according to formula (VI'): wherein A is a monomer comprising a structure according to formula (IV) or (V) wherein each of formula (IV) or (V) It is the point of attachment of the polymer dye backbone; Each D 1 and D 2 are independently selected from aryl, heteroaryl and Each of these are independently selected from substituted or unsubstituted benzene, benzene derivatives, monocyclic aromatic groups, polycyclic aromatic groups, monocyclic heteroaromatic groups and polycyclic heteroaromatic groups, wherein D 1 or D 2 At least one of in yes or its derivatives, said derivatives being included in any available fused to another aryl or heteroaryl group, and wherein The group or its derivatives Any available Location and condense; Each optional M is a polymer modification unit uniformly or randomly distributed along the polymer backbone and is optionally substituted by one or more optionally substituted R 1 , R 2 , R 3 or R 4 Group substitution; Each optional L is a linker; G 1 and G 2 are each independently selected from an unmodified polymer terminus and a modified polymer terminus, optionally conjugated to E; a, c and d define the mol% of each unit within the structure, each of the units may be uniformly or randomly repeated, and wherein each a is from 10% to 100% mol%, each c is from 0% to 90% mol%, and each d is from 0% to 25% mol%; Each b is independently 0 or 1; and p is an integer from 1 to about 10,000; wherein each of T, U, V, X, Y, R 1 ,R 2 ,R 3 ,R 4 ,R 7 ,R 8 ,R 9 ,R 10 ,R 11 ,R 12 ,R 13 ,Q,Z,W1,L 1 , L 2 , L 3 , E, k, f, n, m, n, s, t are as described above. Included in any available Another aryl or heteroaryl group fused to The derivatives may optionally contain Where V' is SO2, SO, S, NR 11 , CR 11 , C(R 11 )2、O、Si(R 11 )2, >C=O, >Se=O, -CH=CH- or -N=CH-, and T, V, X, Y and m are as defined above. In some examples, the monomer of formula (IV) or (V) comprises a structure selected from formula (A1), (A2), (A3), (A4), (A5), (A6), (A7), (A8), (A9), (A10), (A11), (A12), (A13), (A14), (A15), (A16), (A17), (A18), and (A19) as shown herein. In some examples, each G 1 and G 2independently selected from hydrogen, halogen, alkyne, optionally substituted aryl, optionally substituted heteroaryl, aryl substituted with halogen, silyl, diazonium salt, triflate, acetoxy, azide, sulfonate, phosphate, boronic acid substituted aryl, borate substituted aryl, borate, boronic acid, optionally substituted tetrahydropyrene (THP), optionally substituted fluorene, optionally substituted dihydrophenanthrene (DHP), aryl or heteroaryl substituted with one or more side chains terminated with a functional moiety selected from amine, carbamate, carboxylic acid, carboxylate, maleimide, activated ester, N-hydroxysuccinimidyl, hydrazine, hydrazide, hydrazone, azide, alkyne, aldehyde, thiol and protected groups thereof, optionally for conjugation with a substrate or binding partner. In some instances, D 1 and D 2 are the same or different. In some instances, D 1 and D 2 In some instances, D 1 and D 2 are different. The polymers according to the present disclosure may be water soluble. Also provided are methods of preparing the DHP-cyanine polymers and DHP-squarylium polymers of the disclosure. Figure 3 An exemplary synthetic scheme for producing DHP-cyanine polymers according to the present disclosure is shown. Starting with 3,6-dibromo-9,10-substituted-9,10-dihydrophenanthrene 14 Can be produced according to U.S. Patent No. 11,208,527, which is incorporated herein by reference in its entirety. DHP-diindole intermediate 16 The generation of can be carried out by any suitable scheme. For example, DHP-diindole can be produced in a manner similar to Scheme 2 16 Treatment with the appropriate aldehyde diphenylamine hydrochloride in the presence of DIEA and NaOAc results in the formation of DHP-cyanine polymers of formula (IXa) as shown in Scheme 3, wherein p can be an integer from 2 to 1,000, 5 to 500, or 10 to 100. Figure 4 A synthetic scheme 4 for providing DHP-cyanine polymers according to formula (IXb) is shown. Commercially available 3,6-dibromophenanthrene-9,10-dione 12 Reduction with sodium borohydride NaBH4 in water-ethanol provides the 3,6-dibromo-9,10-dihydrophenanthrene-9,10-diol intermediate 13 In the presence of sodium hydride NaH, dibromo-DHP-diol 13Treatment with, for example, an alkyl iodide to provide 3,6-dibromo-DHP-OR 1 Intermediates 14 In the presence of sodium tert-butoxide, Pd(OAc)2, (±)-BINAP, 14 Treatment with benzophenone hydrazone in toluene provides the DHP-dihydrazine intermediate 15 Under acidic conditions, 15 Treatment with methyl isopropyl ketone provides the DHP-diindole intermediate 16 .Will 16 Treatment with 1,3-propane sultone affords the disulfopropyl-naphthoindole intermediate 17 In the presence of acetic anhydride, sodium acetate and DIPEA, 17 Treatment with the appropriate aldehyde diphenylamine hydrochloride (eg, pentadienaldehyde diphenylamine hydrochloride) in CH3CN / CH2Cl2 affords DHP-cyanine dyes of structure (IXb), wherein p can be an integer from 2 to 1,000, 5 to 500, or 10 to 100. V. Capping Unit The linker and the end-capping unit can be conjugated to the fluorescent polymer backbone of the present disclosure by a similar mechanism as previously described. For example, the bromoester and boronate of the end-capping unit can be used to attach one or both ends of the polymer. Both ends of the polymer will be attached using the bromoester and boronate of the end-capping unit. Only one form, i.e., the bromoester or boronate of the end-capping unit, will be attached only to those ends that terminate with their respective complements, and for symmetrical polymerization, can be used to statistically modify only one end of the polymer. For asymmetric polymers, this method is used to chemically ensure that the polymer is modified only at the end of a single strand. The end-capping unit can also be attached asymmetrically by first reacting the bromo end-capping unit with a polymer having a Y end, and then reacting the polymer with a boronate end-capping unit. For example, the capping agents of the present disclosure can be prepared as shown in Scheme (VII): Scheme (VII): VI. Binding Partner A "binding partner" or "specific binding partner" of the present disclosure can be any molecule or molecular complex that is capable of specifically binding to a target analyte. Binding partners of the present disclosure include, for example, proteins, small organic molecules, carbohydrates (including polysaccharides), oligonucleotides, polynucleotides, lipids, affinity ligands, antibodies, antibody fragments, aptamers, and the like. In some embodiments, the binding partner is an antibody or a fragment thereof. In the context of the present disclosure, specific binding refers to a binding reaction that determines the presence of a target analyte in the presence of a heterogeneous population. Thus, under specified assay conditions, a specific binding partner preferentially binds to a specific protein or an isoform of a specific protein, and does not bind to other proteins or other isoforms present in the sample in significant amounts. When the binding partner is an antibody, it can be a monoclonal or polyclonal antibody. The term antibody as used herein refers to immunoglobulin molecules and immunologically active portions of immunoglobulin (Ig) molecules. Such antibodies include, but are not limited to, polyclonal, monoclonal, monospecific polyclonal antibodies, antibody mimics, chimeric, single-chain, Fab, Fab' and F(ab')2 fragments, Fv and Fab expression libraries. Generally, the water-soluble fluorescent compounds and polymers of the present disclosure can be conjugated to a binding partner to form a conjugated water-soluble fluorescent compound or polymer complex using techniques known to those skilled in the art or using methods known in the art in combination with methods described herein. Provided is a labeled specific binding partner comprising a fluorescent compound or polymer according to the present disclosure; and a specific binding partner covalently linked to the fluorescent compound or polymer. The specific binding partner can be an antibody. The specific binding partner can be specific for a target analyte. In some embodiments, the fluorescent compounds or water-soluble fluorescent polymers of the present disclosure can be conjugated to a binding partner using the methods for direct modification of the core polymer described in US2020 / 0190253 (which is incorporated herein by reference in its entirety). For example, the fluorescent compounds or water-soluble fluorescent polymers of the present disclosure can be conjugated to a binding partner according to Scheme 12 ( Fig. 9 ) is shown in the general scheme to prepare DHP-cyanine dye-antibody conjugates. As another example, the DHP-cyanine dye-antibody conjugates can be prepared according to Scheme 13 ( Fig. 9 ) are used to prepare polymer-antibody conjugates. As another example, the polymer-antibody conjugates may be prepared as in Scheme 10 ( Figure 8 ) as shown in the preparation of sulfoxide-bridged DHP-cyanine dye antibody conjugates. For example, the preparation of polymer NHS esters can be carried out as follows. Using a clean vial, 5 mg of polymer is dissolved in 1 mL of dry CH3CN. 15 mg of N, N, N', N'-tetramethyl-O-(N-succinimidyl) uranium tetrafluoroborate (TSTU) is added thereto and stirred for more than 2 minutes. 100 μL of N, N-diisopropylethylamine (DIPEA) is added thereto and stirring is continued overnight, and the lid is sealed with a sealing film. Afterwards, the organic solvent in the reaction mixture is evaporated. The crude NHS is dissolved in about 750 μL of 1×BBS buffer (pH 8.8) by rapid vortexing and transferred to a Zeba column 40K MWCO. The sample is centrifuged (spin-down) at 2200 RPM for 2 minutes, and the polymer NHS is used immediately. Conjugation of polymeric NHS to anti-CD4 antibodies can be performed as follows. Take polymeric NHS in 1× BBS (about 800 μL), centrifuge, add it to 0.6 mg CD4, and mix with 100 μL 0.5 M borate buffer (pH 9.0). Vortex quickly for 30 seconds and allow to mix in a coulter mixer for 3 to 4 hours. Purification of polymer-antibody conjugates by Histrap HP columns can be performed as follows. Method 1: After the crude reaction, the conjugate is purified using a Histrap HP column. The sample is loaded using 1× PBS buffer and the unbound fraction is collected. This can be done using 20CV of buffer. Afterwards, the buffer is changed to wash the bound fraction with both the conjugate and the free antibody. This can be done using 1× PBS containing 0.25M imidazole with a 10CV run. Method 2: SP Sepharose FF column. Use 20mM citrate buffer at pH 3.5 to equilibrate and load the sample, and collect the unbound fraction. This can be done using 20CV of buffer. Afterwards, change the buffer to elute the bound fraction with both conjugate and free antibody. This can be done using 20mM Tris buffer at pH 8.5 with a 20CV run. Method 3: The crude conjugate was loaded in a tangential flow filtration system equipped with a 300K MWCO membrane. The conjugate was washed using 1× PBS until the filtrate showed no absorption at 405 nm or 355 nm. Afterwards, the compound was concentrated. Purification of polymer-antibody conjugates by SEC column can be performed as follows. The crude conjugate containing free antibody is loaded onto a size exclusion column using 1×PBS. After checking the absorption spectrum, the tubes are combined and concentrated in an Amicon Ultra-15 with a 30 KDa MWCO centrifugal concentrator. Purification of polymer-antibody conjugates by Nuvia HR-S columns (Bio-Rad Laboratories, Inc.) can be performed as follows. Using a biological buffer having a pH of about 2 to about 14 and a conductivity of less than 3mS / cm, a crude polymer-antibody conjugate mixture is loaded into a Nuvia HR-S column. Due to the charge-charge interaction between the matrix and the biomolecule, the polymer-antibody conjugate will be bound to the resin, and the free polymer dye will not interact with the resin and will flow through the resin. The charge-charge interaction between the matrix and the polymer-antibody conjugate is destroyed by using a salt (e.g., NaCl, KCl, phosphate, etc.) having a concentration of about 0.1 to 2M. The salt concentration can be reduced by adjusting the pH of the elution buffer. For example, biological buffer and about 100 to 1000mM salt (e.g., NaCl, KCl) concentration gradient can be used to elute the conjugate at a pH of about 6 to about 10. Purification of the conjugate by Nuvia cPrime column (Bio-Rad Laboratories, Inc.) can be performed as follows. Using a biological buffer having a pH of about 2 to about 14 and a salt concentration (e.g., NaCl, KCl) of 0 to about 1M, the crude polymer-antibody conjugate mixture is loaded into the Nuvia cPrime column. Unreacted polymer will flow through the column, and the polymer-antibody conjugate will be combined with the column. The polymer-antibody conjugate is eluted by increasing the pH of the elution buffer. For example, the crude polymer-antibody conjugate can be loaded into the Nuvia cPrime column using a biological buffer of pH 5.0, 5mM NaCl, and eluted with a biological buffer of pH 7.0 and a salt concentration gradient of 5 to 500mM. Purification of the conjugate by anti-mouse anti-H+L antibody-agarose beads can be performed as follows. At room temperature, the crude polymer-antibody conjugate mixture is mixed with anti-mouse anti-H+L antibody-agarose beads in a biological buffer having a pH of about 6 to about 8 for about 30 minutes. Anti-mouse anti-H+L antibody-agarose beads will bind to the polymer-antibody conjugate. Unreacted polymer is removed by washing with the above-mentioned biological buffer at a speed of 300g for 3 minutes using a table centrifuge. Repeat the washing process at least three times. In order to elute the polymer-antibody conjugate, an IgG elution buffer having a pH of about 2 to about 4 is applied to the washed antibody-agarose beads and incubated for about 10 to 15 minutes. Centrifugation is performed to collect the flow-through containing the polymer-antibody conjugate. VII. Methods for Detecting Analytes The present disclosure provides a method for detecting a target analyte in a sample, the method comprising: providing a sample suspected of containing a target analyte; and The sample is contacted with a specific binding partner conjugated to a fluorescent compound or polymer of the disclosure, wherein the binding partner is capable of interacting with the target analyte. A light source that excites the fluorescent compound or polymer can be applied to the sample; and light emitted from the conjugated fluorescent compound or polymer complex is detected. In a typical assay, the water-soluble fluorescent compound or polymer of the present disclosure can be excited at a wavelength of about 420 nm to about 900 nm, and the emitted light is typically about 450 nm to about 1000 nm. Alternatively, the wavelength of the excitation light may be about 500 nm to about 850 nm, and the wavelength of the emitted light may be about 550 nm to about 950 nm. Depending on the design of the compound or polymer, the fluorescent compounds and polymers of the present disclosure may have excitation spectra tuned to UV, violet, blue, yellow, green, red, and NIR or another laser. In the methods of the present disclosure, the fluorescent compound or polymer may be any water-soluble fluorescent compound or polymer of the present disclosure as disclosed herein. A method for detecting a target analyte in a sample is provided, comprising: providing a sample suspected of containing an analyte; and contacting the sample with a specific binding partner conjugated to a fluorescent compound, polymer, or tandem dye according to the present disclosure, wherein the binding partner is capable of interacting with the target analyte. The binding partner can be a protein, a peptide, an affinity ligand, an antibody, an antibody fragment, a carbohydrate, a lipid, a nucleic acid, or an aptamer. When the binding partner is an antibody, the method can be configured for flow cytometry; the water-soluble fluorescent polymer can be bound to a substrate; the analyte can be a protein expressed on the surface of a cell; the method can be configured as an immunoassay; or the method can also include providing additional specific binding partners for simultaneous detection of additional analytes. (VIII) Tandem Dyes The compounds, polymers and labeled specific binding partners of the present disclosure are capable of transferring energy to an attached acceptor chromophore or an attached donor chromophore. The compounds, polymers and labeled specific binding partners of the present disclosure can be covalently linked to an acceptor or donor chromophore in the vicinity of the energy receiving so that excitation of the donor results in energy transfer to and emission from the covalently linked acceptor signaling chromophore. Energy transfer mechanisms between the compounds, polymers and labeled specific binding partners of the present disclosure and the attached donor or acceptor chromophore include, for example, resonance energy transfer (e.g., (or fluorescence) resonance energy transfer ( The term "acceptor chromophore" and "acceptor fluorophore" are used interchangeably herein. The term "donor chromophore" and "donor fluorophore" are used interchangeably herein. Therefore, the present disclosure provides tandem dyes, which include fluorescent compounds, polymers or labeled specific binding partners according to the present disclosure, and acceptor chromophores or donor chromophores covalently linked to fluorescent compounds, polymers or labeled specific binding partners. In some embodiments, the fluorescent compounds, polymers or water-soluble fluorescent compounds or polymers and conjugates thereof of the present disclosure are donor dyes. In some cases, the fluorescent compounds, polymers or water-soluble fluorescent compounds or polymers and conjugates thereof of the present disclosure are acceptor dyes. In some cases, the fluorescent compounds, polymers or water-soluble fluorescent compounds or polymers and conjugates thereof of the present disclosure include other acceptor dyes (e.g., fluorophores or chromophores) connected to donor dyes with the structure of the present disclosure, or other donor dyes (e.g., fluorophores or chromophores) connected to acceptor dyes with the structure of the present disclosure. When the light source excites the donor compound or polymer backbone, the fluorophore, acceptor dye or chromophore can absorb energy of the appropriate wavelength and emit or transfer energy. The fluorophore (FP), chromophore, donor or acceptor dye connected to the fluorescent dye of the present invention can have an absorption or emission spectrum that overlaps to a certain extent with the absorption or emission spectrum of the DHP cyanine compound or polymer of the present disclosure. The FP, chromophore, donor or acceptor dye connected to the fluorescent dye of the present invention can be a fluorescent dye with a maximum absorption longer than 405nm or 575nm and a maximum emission longer than 428nm, 450nm or 600nm, and optionally can exhibit a fluorescence quantum yield greater than 2%, 3%, 4%, 5%, 6%, 7%, 8% or 10%. Any convenient fluorescent dye may be used as the acceptor chromophore or the donor chromophore in the tandem dye. The chromophore and fluorophore may be selected from coumarins, fluorescein, rhodamine, cyanine, bodipy or other polycyclic aromatic hydrocarbons. Many fluorophores are commercially available and may be selected from, but not limited to, any dye available from Beckman Coulter, Inc., including but not limited to SuperNova polymer dyes; any dye available from Becton Dickinson Biosciences, including but not limited to BD Horizon Brilliant TMPolymeric dyes; any dye available from ThermoFisher Scientific, including but not limited to Super Bright polymeric dyes and Alexa Fluor dyes, including but not limited to: Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 568, Alexa Fluor 594, Alexa Fluor 633, Alexa Fluor 647, Alexa Fluor 660, Alexa Fluor 680; ATTO 390, ATTO 465, ATTO 488, ATTO 495, ATTO 514, ATTO 532, ATTO 550, ATTO 565, ATTO 590, ATTO 594, ATTO 610, ATTO 620, ATTO 633, ATTO 647, ATTO 647N, ATTO 655, ATTO 665, ATTO 680, ATTO 700, ATTO 725, ATTO 740 5-Carboxy-2,7-dichlorofluorescein, 5-carboxyfluorescein (5-FAM), 5-carboxynaphthylfluorescein, 5-carboxytetramethylrhodamine (5-TAMRA), 5-FAM (5-carboxyfluorescein), 5-ROX, 6-TAMRA, 6-carboxyrhodamine 6G, 6-CR6G, 6-JOE, 6-FAM, 6-ROX, Bodipy 492 / 515, Bodipy 493 / 503, Bodipy 500 / 510, Bodipy 505 / 515, Bodipy530 / 550, Bodipy 542 / 563, Bodipy 558 / 568, Bodipy 564 / 570, Bodipy 576 / 589, Bodipy581 / 591, Bodipy 630 / 650-X, Bodipy 650 / 665-X,Bodipy 665 / 676,Bodipy Fl,Bodipy R6G,Bodipy TMR,Bodipy TR,CF 488A,CF 555,CF 568,CF 594ST,CF 633,CF640R,CF 647,CF 660C,CF 680,CF680R,CF 750,CF 770,CF 790,CL-NERF,CMFDA,Cy2,Cy3,Cy3.5,Cy5,Cy5.5, Cy7, DDAO, DiA, DiD, DiI, DyLight 488, DyLight 550, DyLight 594, DyLight 633, DyLight 650, DyLight 680, DyLight755, DyLight 800, DiO, DiR, DM-NERF, DsRed, DTAF, DY-490, DY-495, DY-505, DY-530, DY-547, DY-548, DY-549, DY-549P1, DY-550, DY-554, DY-555, DY-556, DY-560, DY-590, DY-591, DY-594, DY-605, DY-610, DY-615, DY-630, DY-631, DY-632, DY-633, DY-634, DY-635, DY-636, DY-647, DY-648, DY-649, DY-649P1, DY-650, DY-651, DY-652, DY-654, DY-675, DY-676, DY-677, DY-678, DY-679, DY-679P1, DY-680, DY-681, DY-682, DY-700, DY-701, DY-703, DY-704, DY-730, DY-731, DY-732, DY-734, DY-749, DY-750, DY-751, DY-752, DY-754, DY-776, DY-777, DY-778, DY-780, DY-781, DY-782, DY-800, DY-831, Eosin, Erythrosin, FITC, Fluo-3, Fluo-4, Fluor-Ruby, FluorX, FM 1-43, FM 1-46, iFluor 488, iFluor 555, iFluor 594, iFluor647, iFluor 680, iFluor 700, iFluor 750, iFluor 780, Lyso Tracker Green, Lyso Tracker Yellow, Mitotracker Green, Mitotracker Orange, Mitotracker Red, NBD, Oregon Green488, Oregon Green 514, PKH26, PKH67, Resorufin, RH 414, Rhod-2, Rhodamine, Rhodamine 110, Rhodamine 123, Rhodamine 6G, Rhodamine B, Rhodamine Green, Rhodamine Red, Rose Bengal, Spectrum Green, Spectrum Orange, Spectrum Red. ,SYTO 11,SYTO 12,SYTO 13,SYTO 14,SYTO 15,SYTO 16,SYTO 17,SYTO 18,SYTO20,SYTO 21,SYTO 22,SYTO 23,SYTO 24,SYTO 25,SYTO 40,SYTO 41,SYTO 42,SYTO 43,SYTO 44,SYTO 45,SYTO 59,SYTO 60,SYTO 61,SYTO 62,SYTO 63,SYTO 64,SYTO 80,SYTO81,SYTO 82,SYTO 83,SYTO 84,SYTO 85,SYTOX Blue,SYTOX Green,SYTOX Orange,TexasRed,Tide Fluor 2(TF2),Tide Fluor 2WS(TF2WS),Tide Fluor 3(TF3),Tide Fluor 3WS (TF3WS), Tide Fluor 4 (TF4), Tide Fluor 5WS (TF5WS), Tide Fluor 6WS (TF6WS), Tide Fluor 7WS (TF7WS), Tide Fluor 8WS (TF8WS), TRITC, and XTRITC. Acceptor dyes useful in the present disclosure may include, for example, cyanine dyes, xanthene dyes, coumarin dyes, thiazine dyes, acridine dyes, FITC, Cy3B, Cy55, Alexa 488, Texas Red, Cy5, Cy7, Alexa 750, Cy55, CY3B, Cy3.5, Alexa 750, 800CW, Biotium CF 555, diethylcoumarin, DY705 (Dyomics), DY431, DY485XL, DY500XL, DY610, DY640, DY654, DY 682, DY 700, DY 701, DY 704, DY 730, DY 731, DY732, DY 734, DY 752, DY 778, DY 782, DY The tandem dye may comprise a DHP cyanine or DHP squarylium compound or polymer according to the present disclosure comprising one or more, two or more, three or more, 1 to 30, 2 to 20 or 2.5 to 10 acceptor dye moieties. The tandem dye may comprise a donor dye and one or more, two or more, three or more, 1 to 30, 2 to 20 or 2.5 to 10 acceptor DHP cyanine or DHP squarylium acceptor dye compounds according to the present disclosure. Fluorescent tandem dyes can be prepared using techniques known to those skilled in the art or using methods known in the art in combination with the methods described herein. The tandem dyes can be water soluble. In some embodiments, rather than being attached to a compound or polymer backbone, acceptor dyes, chromophores, fluorophores, functional moieties, and binding partners may be attached to a compound or polymer of the present disclosure via a linker moiety using methods for direct modification of core polymers as described in U.S. Pat. No. 11,584,825, which is incorporated herein by reference in its entirety. In some embodiments, the present disclosure provides a tandem dye comprising: a fluorescent compound, polymer, or labeled specific binding partner according to the present disclosure; and an acceptor chromophore covalently linked to the fluorescent compound, polymer, or labeled specific binding partner. sample The sample in the method of the present disclosure can be, for example, blood, bone marrow, spleen cells, lymphocytes, bone marrow aspirates (or any cells obtained from the bone marrow), urine (lavage fluid), serum, saliva, cerebrospinal fluid, urine, amniotic fluid, interstitial fluid, feces, mucus or tissue (e.g., tumor samples, decomposed tissues, decomposed solid tumors). In certain embodiments, the sample is a blood sample. In some embodiments, the blood sample is whole blood. Whole blood can be obtained from a subject using standard clinical procedures. In some embodiments, the sample is a subpopulation of one or more cells of whole blood (e.g., red blood cells, white blood cells, lymphocytes (e.g., T cells, B cells or NK cells), phagocytes, monocytes, macrophages, granulocytes, basophils, neutrophils, eosinophils, platelets or any cells with one or more detectable markers). In some embodiments, the sample can be from a cell culture. The subject can be a human (e.g., a patient suffering from a disease), a commercially important mammal, including, for example, a monkey, a cow, or a horse. The sample can also be obtained from a household pet, including, for example, a dog or a cat. In some embodiments, the subject is a laboratory animal used as an animal model for disease or for drug screening, such as a mouse, a rat, a rabbit, or a guinea pig. Analytes As used herein, "analyte" or "target analyte" refers to a substance, such as a molecule, whose abundance / concentration is determined by some analytical procedure. For example, in the present disclosure, an analyte can be a protein, peptide, nucleic acid, lipid, carbohydrate small molecule, or target-related biomolecule. The target analyte can be, for example, a nucleic acid (DNA, RNA, mRNA, tRNA or rRNA), a peptide, a polypeptide, a protein, a lipid, an ion, a monosaccharide, an oligosaccharide, a polysaccharide, a lipoprotein, a glycoprotein, a glycolipid or a fragment thereof. In some embodiments, the target analyte is a protein, and can be, for example, a structural microfilament, a microtubule and an intermediate filament protein, an organelle-specific marker, a proteasome, a transmembrane protein, a surface receptor, a nucleoporin, a protein / peptide translocase, a protein folding partner, a signaling scaffold, an ion channel, etc. The protein can be an activatable protein or a protein differentially expressed or activated in a diseased or abnormal cell, including but not limited to a transcription factor, a DNA and / or RNA binding protein and a modified protein, a nuclear import and export receptor, an apoptosis or survival regulator, etc. Compounds, polymers, labeled specific binding partners or tandem dyes according to the present disclosure may be water-soluble fluorescent dyes. Fluorescent compounds, labeled specific binding partners or tandem dyes according to the present disclosure may exhibit a solubility in water of >1 mg / mL, >2 mg / mL, >3 mg / mL, >4 mg / mL, >5 mg / mL, >6 mg / mL, >7 mg / mL, >8 mg / mL, >9 mg / mL, >10 mg / mL, >20 mg / mL, >30 mg / mL, >40 mg / mL, >50 mg / mL, >80 mg / mL or >10 mg / mL at ambient room temperature. Compounds, polymers, labeled specific binding partners, or tandem dyes according to the present disclosure may exhibit an excitation maximum wavelength (λex) of >400 nm, >500 nm, >600 nm, >700 nm, >800 nm, >850 nm, or in the range of about 400 nm to about 1,000 nm or about 600 nm to about 950 nm. DHP cyanine compounds, polymers, labeled specific binding partners, or DHP cyanine acceptor dyes comprising DHP cyanine compounds according to the present disclosure may exhibit an emission maximum (λem) of >550 nm, >650 nm, >750 nm, >850 nm, or >900 nm, or in the range of about 600 to about 1200, or about 550 to about 1050 nm, or about 650 to about 1050 nm. Determination DHP cyanine dyes, tandem dyes, labeled specific binding partners, compositions, methods and systems as described herein can be used in a variety of applications, including diagnostic and research applications, where it is desirable to label, detect and / or analyze a target of interest. Such applications include, for example, methods such as cytometry, microscopy, immunoassays (e.g., competitive or non-competitive), fluorescence in situ hybridization (FISH), cell tracing, receptor labeling, fluorescence spectroscopy, free analyte evaluation, receptor binding ligand evaluation, and the like. The compositions, systems and methods described herein can be useful in analyzing any of a number of samples, including, but not limited to, biological fluids, cell culture samples, and tissue samples. In certain aspects, the compositions, systems and methods described herein can be used in methods where a fluorescent marker is used to detect an analyte (if present) in a sample, such as for fluorescence-activated cell sorting or analysis, immunoassays, immunostaining, and the like. In some cases, the compositions and methods can be used in applications where the presence of a target analyte of interest in a sample is evaluated. In some cases, the methods and compositions can be used for any assay for detecting and / or analyzing a target from a sample of interest, including but not limited to flow cytometry, fluorescence microscopy, in situ hybridization, enzyme-linked immunosorbent assay (ELISA), western blot analysis, magnetic cell separation assays, and fluorescent dye purification chromatography. In some cases, the methods and compositions can be used for any application in which a target molecule of interest is fluorescently labeled. The subject composition can be suitable for any convenient application in which paired specific binding members, such as biotin-streptavidin and hapten-antihapten antibodies, can be used. Assay systems that utilize binding partners and fluorescent labels to quantify binding molecules are well known. Some examples of such systems include flow cytometry, scanning cytometry, imaging cytometry, fluorescence microscopy, and confocal fluorescence microscopy. In some embodiments, flow cytometry is used to detect fluorescence. Many devices suitable for this use are available and known to those skilled in the art. Some examples include BCI Navios, Gallios, Aquios, and CytoFLEX flow cytometers. In other embodiments, an assay is used. The assay may be an immunoassay. Some examples of immunoassays that may be used in the present disclosure include, but are not limited to, fluoroluminescence assays (FLA) and the like. The assay may also be performed on a protein array. When the binding partner is an antibody, an antibody or multi-antibody sandwich assay may also be used. A sandwich assay refers to the use of sequential recognition events to construct a layer of multiple binding partners and reporter elements to signal the presence of a specific analyte. Some examples of sandwich assays are disclosed in U.S. Pat. No. 4,486,530 and references cited therein. (IX) Kit The present disclosure provides kits comprising at least one DHP cyanine or DHP squaraine compound or polymer according to the present disclosure, a labeled specific binding partner or a tandem dye. Some aspects of the present invention also include kits for implementing the subject methods and compositions. The compositions of the present invention can be included in the kit as a reagent, as a starting material or provided for use in, for example, the above methods. The kit may include a DHP cyanine or DHP squaryl cyanine compound or polymer as described herein, a labeled specific binding partner or tandem dye, and a container. Any convenient container may be used, such as a tube, a bottle, or a hole in a porous strip or a porous plate, a box, a bag, an insulated container, etc. In some cases, the subject kit may include one or more components and / or instructions for use selected from the following: a DHP cyanine or DHP squaryl cyanine compound or polymer according to the present disclosure, a labeled specific binding partner or tandem dye, a fluorophore, a chromophore, a specific binding member, a specific binding member conjugate, a support-bound specific binding member, a cell, a support, a biocompatible aqueous elution buffer. In some embodiments of the kit, a DHP-cyanine or DHP-squaryl cyanine compound or polymer or tandem dye according to the present disclosure is covalently linked to a specific binding partner. In some cases, the subject kit may be a "labeling kit" comprising a DHP-cyanine or DHP-squaryl cyanine compound or polymer or a tandem dye according to the present disclosure, comprising a side chain chemoselective functional group (also referred to as a "conjugated tag"), such as, for example, an NHS ester of a DHP-cyanine or DHP-squaryl cyanine compound or polymer, to which any convenient target moiety of interest (e.g., a donor or acceptor dye, a fluorophore, a chromophore, a specific binding partner, a support) may be conjugated. The chemoselective functional group may include a reactive group (e.g., biotin) that targets a specific functional group on a biomolecule (e.g., a protein or antibody), such as, for example, a primary amine, a sulfhydryl, a carboxyl, or a carbohydrate. The chemoselective functional group may be a functional group for a "click chemistry" reaction. In some cases, the conjugated tag includes a maleimide functional group, and the target moiety includes a thiol functional group, or vice versa. In some cases, the conjugated tag includes an alkyne (e.g., a cyclooctyl group) functional group, and the target moiety includes an azide functional group, or vice versa, which can be conjugated by click chemistry. In some cases, the conjugated tag includes an olefin (e.g., a cyclooctyl group) functional group, and the target moiety includes a tetrazine functional group, or vice versa, which can be conjugated by a reverse demand Diels–Alder cycloaddition reaction. In some cases, the conjugated tag includes an amine-reactive chemical group, such as, for example, an NHS ester (N-hydroxysuccinimide ester) or an imidoester functional group, and the target moiety includes an NH2 functional group, or vice versa. In some cases, the conjugated tag includes a biotin binding protein (e.g., avidin, streptavidin, or neutravidin), and the target moiety includes a biotin molecule, or vice versa, which can interact non-covalently. Example Example 1. General Procedure for the Synthesis of DHP Indole-Cyanine Compounds 1 to 5 The synthetic procedures used to synthesize DHP indole-cyanine compounds are Figure 2 , as shown in Scheme 2. The synthetic procedures for preparing compounds 1 to 5 as shown in Table 1 are shown below. DHP-diol intermediate 7 Synthesis of 3-bromo-9,10-dihydrophenanthrene-9,10-diol: According to a previously published procedure, commercially available 3-bromoDHP-dione was reduced using NaBH4 in 85% yield (US11208527B2). DHP-OMe intermediate 8 Synthesis of 3-bromo-9,10-dimethoxy-9,10-dihydrophenanthrene: To the nitrogen-purged THF solution of DHP-diol (1mmol) in ice, NaH (4mmol) was added. After 5 minutes, iodomethane solution was added dropwise. The reactant was stirred at room temperature overnight. The next day, a few drops of water were used to quench the reaction. After evaporating THF, chloroform was used to extract the reaction mixture. After column chromatography, pure compound was obtained with a yield of 80%. DHP-hydrazone intermediate 9 Synthesis of 1-(9,10-dimethoxy-9,10-dihydrophenanthrene-3-yl)-2-(diphenylmethylene)hydrazine: Benzophenone hydrazone (1.50 mmol), Pd(OAc)2 (0.015 mmol), and (±)-BINAP (12.7 mg, 0.020 mmol; (2,2'-bis(diphenylphosphino)-1,1'-binaphthyl)) were suspended in toluene (2 mL). The reaction vessel was briefly purged with argon and stirred for 3 minutes. To the resulting purple solution were added the DHP derivative (1.36 mmol), NaOt-Bu (1.90 mmol), and toluene (0.4 mL). The reaction vessel was heated at 100 °C overnight. Afterwards, the reaction mixture was cooled to 25 °C and filtered through a short pad of diatomaceous earth. The clear filtrate was concentrated and the residue was purified by flash column chromatography with a yield of 60%. DHP-Indole intermediate 10 Synthesis of 5,6-dimethoxy-8,8,9-trimethyl-6,8-dihydro-5H-naphtho[2,1-f]indole: DHP-hydrazone (1.35 mmol), isopropyl methyl ketone (2.03 mmol) and TsOH*H2O (6.75 mmol) were heated to reflux in ethanol (20 mL) for 15 hours. The crude product was purified by flash column chromatography to give the DHP-indole derivative in 25% yield. DHP-Indolesulfonate intermediate 11 Synthesis of 3-(5,6-dimethoxy-8,8,9-trimethyl-6,8-dihydro-5H-1014-naphtho[2,1-f]indol-10-yl)propane-1-sulfonate: The DHP-indole intermediate (1 mmol) 10 was heated with 1,3-propane sultone at 120 degrees Celsius for 2 hours. Afterwards, the reaction mixture was cooled, washed with ether and dried to provide the sulfonated DHP-indole intermediate compound 11 . Synthetic compounds 1 to 3 General procedures Sulfonated DHP indole (1 mmol) 11The solution in MeCN is added to the reflux mixture of corresponding aldehyde diphenylamine hydrochloride (1.20mmol), Ac o (120 mL), DIEA (600 mL) and NaOAc (78 mg) in MeCN / DCM mixed solvent. The gained mixture was stirred for another 2 hours, then excessive sulfonated DHP indole (1.50 mmol) was added. The mixture was refluxed again for 2 hours. Reaction was quenched by water, and the solvent was removed by rotary evaporator. Residue was washed with EtOAc, MeCN, EtOAc and water successively, then purified by column chromatography, to obtain compounds 1 to 3 as shown in table 1. Synthetic compounds 4 to 5 General procedures Sulfonated DHP indole (1 mmol) 11 The solution in MeCN was added to a refluxing mixture of the corresponding aldehyde diphenylamine hydrochloride (1.20 mmol), Ac2O (120 mL), DIEA (600 μL) and NaOAc (78 mg) in a MeCN / DCM mixed solvent. The resulting mixture was stirred for another 2 hours before an excess of 1-(4-sulfobutyl)-2,3,3-trimethyl-4,5-benzindole was added. The mixture was refluxed for 2 h. The reaction was quenched with water and the solvent was removed by rotary evaporator. The residue was washed with EtOAc, MeCN, EtOAc and water in turn and then purified by column chromatography to obtain compound 4 or 5 . Compounds 1 to 5 can be derivatized with sulfonamide PEG side groups to improve water solubility. For example, any of compounds 1 to 5 can be treated with thionyl chloride in DMF to form a propanesulfonyl chloride intermediate, which can then be treated with NH2PEG in CH2Cl2. 550 OMe, TEA treated to form sulfonamide PEG side groups. Example 2. DHP-indolepropyl sulfonate intermediate 21 Synthesis Figure 6 The preparation of N-propyl sulfonate DHP-indole compound from dihydrophenanthrene is shown 21 Synthesis Scheme 7. 2-Nitro DHP intermediate 17 Synthesis of 2-nitro-9,10-dihydrophenanthrene: To 1 mmol 9,10-dihydrophenanthrene, 8 mL acetic acid and 2 mL fuming nitric acid (20%) were added. The mixture was stirred and the temperature was maintained at 30 degrees Celsius. After 2 hours, the red solution was poured into crushed ice. The precipitation was completed and the aqueous layer was decanted. It was washed with more cold water (500 mL). Dry and the obtained 2- and 4-nitro 9,10-dihydrophenanthrene 17 in Used in the next step without further purification. 9,10-Dihydrophenanthrene-2-amine intermediate 18 Synthesis of: Na2S (3 mmol) was dissolved in a minimum amount of hot water and mixed with 240 mg of Na2CO3. This was added to 2.2 mmol of nitro-9,10-dihydrophenanthrene 17 in methanol. The mixture was heated overnight. The next day, it was extracted with chloroform and dried. Chromatography was performed to separate 2- and 4-amino-9,10-dihydrophenanthrene. 18 . (9,10-Dihydrophenanthrene-2-yl)hydrazine intermediate 19 Synthesis of: 2-amino-9,10-dihydrophenanthrene 18 To an ice-cold suspension of (1 mmol) in 20% HCl (2 mL) was added sodium nitrite (100 mg in 250 μL) dropwise. Stirring was continued at 0 degrees Celsius for 1 hour. A small amount of urea was added to decompose the excess nitrous acid. The temperature was maintained at -5 degrees Celsius. To the diazonium salt solution was added SnCl2.2H2O (250 mg) in a minimum amount of concentrated HCl and stirred for 2 hours. Afterwards, the precipitate was filtered and dried. Afterwards, the precipitate was dissolved in chloroform and extracted with water containing 10% NaOH. The organic layer was dried and then chromatographed to obtain 19 . 2,3,3-Trimethyl-4,5-dihydro-3H-naphtho[2,1-e]indole intermediate 20 Synthesis of: Dissolve 2-hydrazino-9,10-dihydrophenanthrene in 5 mL of glacial acetic acid. 19 (1 mmol). It was mixed with isopropyl methyl ketone (2 mmol) and the reaction was carried out at 110 degrees Celsius overnight. The next day, ethyl acetate and water were extracted and then chromatographed to obtain 20 . DHP-Indolepropyl Sulfonate Intermediate 21 Synthesis of 3-(2,3,3-trimethyl-4,5-dihydro-3H-naphtho[2,1-e]indole-1- -1-yl) propane-1-sulfonate intermediate 21: The dihydrophenanthrenol derivative obtained in the previous step 20 (1 mmol) was heated with 1,3-propane sultone at 120 degrees Celsius for 2 hours. After cooling, the product was washed with ethyl acetate to remove any unreacted starting material to obtain 21 . Example 3. Sulfoxide-bridged DHP-indocyanine antibody 25 Synthesis Figure 7 The intermediate compound DHP-indole from -propyl sulfonate is shown 21 Preparation of Sulfoxide-bridged DHP Indocyanine Dyes 25 Synthetic Schemes 8 and 9. Sulfoxide-bridged DHP-indolepropylsulfonate intermediate 22 Synthesis of 2-((l1-oxoalkyl)dioxo-l6-sulfanyl)-6-(3-((l1-oxoalkyl)dioxo-l6-sulfanyl)propyl)-7,8,8-trimethyl-9,10-dihydro-8H-6l4-thieno[2',3',4',5':4,5]phenanthro[2,1-b]pyrrole 4,4-dioxide 22 : 2-Aminodihydrophenanthrene 18 was reacted using NaNO2 / HCl in an ice bath to convert into the corresponding diazonium salt. It formed 2-hydrazinodihydrophenanthrene (19) when reacted with SnCl2 / HCl at 0 to 5 degrees Celsius. The product was used in the next step without further purification. Thereafter, 19 was reacted with 3-methyl-2-butanone under Fisher indole synthesis conditions to produce the isomer of DHP indole (20). The formation of the indole ring can occur at the 1 and 2 positions as well as at the 2 and 3 positions of DHP. The two isomers were separated using flash chromatography. Take the indole formed at the 1,2-position of DHP and react it with 1,3-propanesultone to obtain the intermediate DHP-indole compound 21 , yield 100%. It was further treated with 20% oleum precooled at -20 degrees Celsius, and the reaction was continued at room temperature overnight. The crude product was precipitated with ether and then subjected to C18 reverse phase chromatography (100% water to 95% water 5% methanol) To obtain the compound 22 . Sulfoxide-bridged DHP-indocyanine dyes 25Synthesis of (Z)-2-((2E,4E)-5-(2-((l1-oxoalkyl)dioxo-l6-sulfanyl)-6-(3-((l1-oxoalkyl)dioxo-l6-sulfanyl)propyl)-8,8-dimethyl-4,4-dioxo-9,10-dihydro-8H-6l4-thieno[2',3',4',5':4,5]phenanthro[2,1-b]pyrrol-7-yl)penta-2,4-diene-1-ylidene)-1-(3-((l1-oxoalkyl)dioxo-l6-sulfanyl) (propyl)-3,3-dimethylindoline-5-carboxylic acid 25 : Sulfonated indole functionalized with carboxylic acid ( 23 )and 24 Treatment of sulfoxide-bridged DHP indole ( 22 After the reaction, the crude mixture was mixed with cold ether and the precipitate was separated. It was purified using C18 reverse phase chromatography (20% MeOH-80% water) to obtain DHP-sulfoxide Cy5 dye 25 It was found to exhibit a maximum Abs of 666 nm, a maximum emission of 686 nm and an extinction coefficient of 150450 nm. -1 cm -1 . Example 4. Sulfoxide-bridged DHP-indocyanine antibody conjugate 27 Synthesis Figure 8 Shown is a DHP indocyanine dye bridged by a sulfoxide 25 Preparation of sulfoxide-bridged DHP indocyanine dye-antibody conjugates 27 Synthesis Scheme 10. Sulfoxide-bridged DHP-indocyanine dye antibody conjugate 27 Synthesis of: DHP-sulfoxide Cy5 dye ( 25 ) into active NHS ester 26 It is purified by precipitation technique using diethyl ether. At pH about 7.5 to 8, the active ester 26 The unreacted excess dye was separated using a size exclusion spin column and the resulting pure conjugate ( 27 ) for flow cytometry studies. Example 5. DHP-indole intermediate 33Synthesis of: 1-(3-((λ1-oxoalkyl)dioxo-16-sulfanyl)propyl)-2,3,3,5,5-pentamethyl-4,5-dihydro-3H-1λ4-naphtho[2,1-e]indole 33 Fig.10 The preparation of N-propylsulfonate DHP-indole intermediate compound from chloroaminobenzoic acid 28 is shown 33 Exemplary synthetic scheme 14. Fig.10 Also shown is the DHP-indole intermediate compound from N-propyl sulfonate 33 Preparation of Sulfoxide-bridged DHP Indocyanine Dyes 36 Example routes. 2-Chloro-4-hydrazinobenzoic acid 29 Preparation At 0 degrees Celsius, chloroaminobenzoic acid 28 Mix with 20% HCl. Add NaNO2 (1 eq.) in water and stir for 1 hour. Then, add excess urea and stir for 5 minutes. The reaction mixture is further cooled to about -5 degrees Celsius. Add tin chloride (2.1 eq.) in concentrated HCl and stir for another 2 hours. Then, pour the reaction mixture into ice-cold water and filter to obtain hydrazine compound. 29 . Preparation of 5-chloro-2,3,3-trimethyl-3H-indole-4-carboxylic acid 30 Hydrazine compounds 29 Heated with 3-methyl-2-butanone at 120 degrees Celsius overnight The crude product precipitated the next day, which was washed repeatedly with EtOAc and then chromatographed on silica gel to give the indole product 30 as a mixture of isomers. Preparation of 2,3,3,5,5-pentamethyl-4,5-dihydro-3H-naphtho[2,1-e]indole 31 Under Schlenk conditions at 140 °C, in the presence of Pd(OAc)2, K2CO3, tri(o-tolyl)phosphine (P(p-tolyl)3) and tetrabutylammonium bromide in DMF, in the presence of indole derivatives 30 The reaction was carried out overnight with 1-(tert-butyl)-2-iodobenzene. The next day, the crude product was purified by column chromatography. 31 . Preparation of 1-(3-((11-oxoalkyl)dioxo-16-sulfanyl)propyl)-2,3,3,5,5-pentamethyl-4,5-dihydro-3H-114-naphtho[2,1-e]indole 33 Compound 31 With 1 equivalent of 1,3-propanesultone in THF 32 Mix in a sealed tube at 120 degrees Celsius for 90 minutes. 33 The precipitate was washed with cold ether. Sulfoxide-bridged DHP-indolepropyl sulfonate intermediate 34 The synthesis of 2-((l1-oxoalkyl)dioxo-l6- Sulfanyl)-6-(3-((l1-oxoalkyl)dioxo-l6-sulfanyl)propyl)-7,8,8,10,10-pentamethyl-9,10-di Hydrogen-8H-6l4-thieno[2',3',4',5':4,5]phenanthro[2,1-b]pyrrole 4,4-dioxide. The sulfoxide-bridged DHP-indolepropyl sulfonate intermediate compound 34 is prepared by reacting the intermediate compound 33 with the above-mentioned synthetic compound 22 Prepared in a similar manner. Synthesis of sulfoxide-bridged DHP-indocyanine dye 36, 2-((l1-oxoalkyl)dioxo-l6-sulfanyl)-7-((1E,3E)-5-((Z)-5-((l1-oxoalkyl)dioxo-l6-sulfanyl)-1-(5-carboxypentyl)-3,3-dimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-6-(3-((l1-oxoalkyl)dioxo-l6-sulfanyl)propyl)-8,8,10,10-tetramethyl-9,10-dihydro-8H-thieno[2',3',4',5':4,5]phenanthro[2,1-b]pyrrole-6-yl)- 4,4-Dioxide. In a mixture of acetic acid and acetic anhydride, a sulfonated indole ( 35 ) treatment of sulfoxide-bridged DHP indole ( 34 After the reaction, the crude mixture was mixed with cold ether and the precipitate was separated. It was purified using C18 reverse phase chromatography to obtain DHP-sulfoxide Cy5 dye 36 , it was found that it showed a maximum Abs of 663nm and a maximum emission of 683nm. The above description, examples and data provide a complete description of the manufacture and use of the composition of the present invention. Due to the many embodiments of the present invention that can be carried out without departing from the spirit and scope of the present invention, the present invention is included in the claims hereinafter attached.

Claims

1. A fluorescent compound comprising a structure according to formula (II): in: Each are independently selected from substituted or unsubstituted benzene, benzene derivatives, monocyclic aromatic groups, polycyclic aromatic groups, monocyclic heteroaromatic groups and polycyclic heteroaromatic groups, At least one of Include or its derivatives, wherein the derivative is optionally contained in any available fused to an additional aryl or heteroaryl group, optionally wherein the derivative is in: Each V' is independently SO2, SO, S, NR 11 , CR 11 , C(R 11 )2、O、Si(R 11 )2. >C=O, >Se=O, -CH=CH- or -N=CH-; Each T is independently selected from C, C(R 1 )、N、N(R 1 )、P、O、S and Si(R 1 ); Each U is independently selected from NR 10 , O, P, Se, Te and S; Each V is independently selected from NR 11 , CR 11 , C(R 11 )2, S, SO2, O, Se, Te and Si(R 11 )2; Each X is independently CR 1 R 2 or SiR 1 R 2 ; Each Y is independently CR 8 R 9 or SiR 8 R 9 ; in: Each R 1 , R 2 , R 8 and R 9 independently selected from a water soluble moiety, a linked water soluble moiety, a linker moiety, a linked E, a reactive group, a linked reactive group, a binding partner, a linked binding partner, a functional group, hydrogen, hydroxyl, halogen, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, haloalkyl, aryl, heteroaryl, (hetero)aryloxy, (hetero)arylamino, sulfonamide-PEG, phosphoamide-PEG, alkylammonium salt, alkyloxyammonium salt, oligoether ammonium salt, carbonyl, acyl, alkylsulfonate, alkoxysulfonate, oligoethersulfonate, sulfonamidooligoether, sulfonamide, sulfenamide, phosphoamidate, phosphinamide, alkoxysulfonamide PEG, alkoxysulfonate, alkylsulfonate, alkylsulfonate, alkylsulfonate, Optionally, wherein R 1 and R 8 together form an unsubstituted or substituted cycloalkyl, cycloalkenyl, heterocycloalkyl, heterocycloalkenyl, cycloalkoxy, aryl or heteroaryl group having 3 to 9 ring members; Each R 3 independently selected from H, alkyl, alkene, alkyne, cycloalkyl, haloalkyl, alkoxy, (hetero)aryloxy, aryl, (hetero)arylamino, a water soluble moiety, a chromophore, a functional moiety, a binding partner, and a PEG group; Each Q is independently a bond, NH, NR 4 、C1-C 12 Alkylene, CHR 4 or CH2; Each Z is independently CH2, CHR 4 , O, NR 4 or NH; Each W 1 independently a water-soluble portion; L 1 , L 2 and L 3 are each independently selected linker moieties; Each E is independently selected from a chromophore, a functional moiety, a substrate, and a binding partner; Each R 4 independently selected from H, alkyl, PEG, a water soluble moiety, a linker moiety, a chromophore, a functional group, a conjugated tag, a carboxylic acid amide, an amine, a carbamate, a carboxylic acid, a carboxylate, a maleimide, an activated ester, an N-hydroxysuccinimidyl group, a hydrazine, a hydrazone, an azide, an alkyne, an alkene, a tetrazine, an aldehyde, and a thiol, or a protected group thereof, Each R 7 Independently selected from H, hydroxyl, C1-C 12 Alkyl, C2-C 12 Olefins, C2-C 12 Alkynes, C3-C 12 Cycloalkyl, C1-C 12 Halogenated alkyl, C1-C 12 Alkoxy, C2-C 18 (Hetero)aryloxy, C2-C 18 (Hetero)arylamino, C2-C 12 Carboxylic acid, C2-C 12 Carboxylic acid esters and C1-C 12 Alkoxy, functional group, chemoselective functional group, conjugated tag, attached conjugated tag, linker, sulfonic acid, sulfonate, C1-C 12 Alkyl sulfonates / esters, sulfonamides; Each R 10 independently selected from hydrogen, a linker moiety, an attached reactive group, a conjugated tag, an attached conjugated tag, an attached ionic group, an attached chromophore, an attached water-soluble moiety, a water-soluble moiety, a chromophore, a binding partner, a attached binding partner; Each R 11 independently selected from hydrogen, a linker moiety, an attached reactive group, an attached ionic group, an attached chromophore, an attached water-soluble moiety, a chromophore, a binding partner, and an attached binding partner; Each R 12 , R 13 and R 14 independently selected from hydrogen, halogen, substituted or unsubstituted C1-C6 alkyl, substituted or unsubstituted C1-C6 heteroalkyl, substituted or unsubstituted C1-C6 alkene, substituted or unsubstituted C3-C 10 cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, and substituted or unsubstituted heteroaryl, CO2R 1 ,CONR 1 R 2 、-O-CH2CH2-PEG-R 7 、-S-CH2CH2-PEG-R 7 、-N-CH2CH2-PEG-R 7 , O-aryl, S-aryl, N-aryl, -O-alkyl, S-alkyl, N-alkyl, wherein each alkyl or aryl group may be optionally replaced by one or more R 7 , PEG, PEG-R 7 or a linking group, optionally wherein each R 12 , R 13 and R 14 Independently by R 7 Group substitution; or R 10 , R 11 , R 12 , R 13 and R 14 At least two of the 10 Cycloalkyl, unsubstituted or substituted, unsaturated or partially unsaturated C3-C 10 heterocycloalkyl, which is optionally substituted by O, unsubstituted or substituted unsaturated or partially unsaturated cycloalkyl having 3 to 8 ring members, or substituted or unsubstituted heterocycloalkyl having 3 to 8 ring members, which is optionally substituted by O; Each K is independently a covalent bond, O, S, P, NR 1 , Se, Te, CR 1 R 2 or CH2; Each f is independently an integer from 0 to 50; Each m is independently 0 or 1; Each n is independently 0, 1, 2, 3 or 4; Each s is independently 1 or 2; and Each t is independently 0, 1, 2 or 3.

2. The compound of claim 1, which is selected from the group consisting of formula (IIa), (IIb), (IIc), (IId), (IIe), (IIf), (IIg), (IIk) and (IIl):

3. The compound according to claim 1 or 2, wherein Groups contain the same A group or a derivative thereof, optionally wherein The derivatives are included in any available The additional aryl or heteroaryl group fused to 4. The compound according to claim 1 or 2, wherein The group contains different A group or a derivative thereof, optionally wherein The derivatives are included in any available The additional aryl or heteroaryl group fused to 5. The compound of claim 1, wherein is unsubstituted or substituted benzene or unsubstituted or substituted naphthalene; U is N; and V is CR 11 or C(R 11 )2, N, O or S.

6. The compound according to any one of claims 1 to 5, wherein at least one The group or its derivatives are selected from: Each R 5 independently selected from halogen, hydroxyl, C1-C 12 Alkyl, C2-C 12 Olefins, C2-C 12 Alkynes, C3-C 12 Cycloalkyl, C1-C 12 Halogenated alkyl, C1-C 12 Alkoxy, C2-C 18 (Hetero)aryl, C2-C 18 (Hetero)aryloxy, C2-C 18 (Hetero)arylamino, carboxylic acid, carboxylate, (CH2) x’ (OCH2-CH2) y’ OCH3 and (CH2) x’ (OCH2-CH2) y’ OCF3, wherein each x' is independently an integer from 0 to 20, and each y' is independently an integer from 0 to 50.

7. A compound according to any one of claims 1 to 6, which is selected from the formula (IIIa), (IIIb), (IIIc), (IIId), (IIIe), (IIIf), (IIIy), (IIIbb), (IIIcc), (IIIdd) and (IIIee):

8. The compound of any one of claims 1 to 7, wherein each R 10 and each R 11 independently selected from a water soluble moiety, a linker moiety, a linked water soluble moiety, hydrogen, an alkyl, an alkene, an alkyne, a cycloalkyl, a haloalkyl, a (hetero)aryloxy, an aryl, a heteroaryl, a (hetero)arylamino, a sulfonamide-PEG, a phosphoamide-PEG, an alkylammonium salt, an alkyloxyammonium salt, an oligoether ammonium salt, an alkylsulfonate, an alkoxysulfonate, an oligoethersulfonate, a sulfonamidooligoether, a sulfonamide, a sulfenamide, a phosphoamidoester, a phosphinamide, an alkoxysulfonamide PEG, an alkoxysulfonate, an alkylsulfonate, an alkylsulfonate, a 9. The compound of any one of claims 1 to 8, comprising a structure according to any one of:

10. An acceptor dye comprising the structure according to any one of claims 1 to 9.

11. A polymeric dye comprising a monomer having a structure according to formula (IV) or (V): in: Each of (IV) or (V) It is the point of attachment to the polymer dye backbone; Each D 1 and D 2 are independently selected from aryl, heteroaryl and in Each are independently selected from substituted or unsubstituted benzene, benzene derivatives, monocyclic aromatic groups, polycyclic aromatic groups, monocyclic heteroaromatic groups and polycyclic heteroaromatic groups, wherein D 1 or D 2 At least one of in yes or its derivatives, said derivatives being included in any available Another aryl or heteroaryl group fused to Optionally, wherein the derivative thereof comprises And among them The group or its derivatives Any available Location and condense; Each V' is independently SO2, SO, S, NR 11 , CR 11 , C(R 11 )2、O、Si(R 11 )2. >C=O, >Se=O, -CH=CH- or -N=CH-; Each T is independently selected from C, C(R 1 ), N, P, O, S, and Si(R 1 ); Each U is independently selected from NR 10 , O, P, Se, Te and S; Each V is independently selected from NR 11 , CR 11 , C(R 11 )2, S, SO2, O, Se, Te and Si(R 11 )2; Each X is independently CR 1 R 2 or SiR 1 R 2 ; Each Y is independently CR 8 R 9 or SiR 8 R 9 ; in: Each R 1 , R 2 , R 8 and R 9 independently selected from a water soluble moiety, a linked water soluble moiety, a linker moiety, a linked E, a reactive group, a linked reactive group, a binding partner, a linked binding partner, a functional group, hydrogen, hydroxyl, halogen, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, haloalkyl, aryl, heteroaryl, (hetero)aryloxy, (hetero)arylamino, sulfonamide-PEG, phosphoamide-PEG, alkylammonium salt, alkyloxyammonium salt, oligoether ammonium salt, carbonyl, acyl, alkylsulfonate, alkoxysulfonate, oligoethersulfonate, sulfonamidooligoether, sulfonamide, sulfenamide, phosphoamidate, phosphinamide, alkoxysulfonamide PEG, alkoxysulfonate, alkylsulfonate, alkylsulfonate, alkylsulfonate, Optionally, wherein R 1 and R 8 together form an unsubstituted or substituted cycloalkyl, cycloalkenyl, heterocycloalkyl, heterocycloalkenyl, cycloalkoxy, aryl or heteroaryl group having 3 to 9 ring members; Each R 3 independently selected from H, alkyl, alkene, alkyne, cycloalkyl, haloalkyl, alkoxy, (hetero)aryloxy, aryl, (hetero)arylamino, a water soluble moiety, a chromophore, a functional moiety, a binding partner, and a PEG group; Each Q is independently a bond, NH, NR 4 、C1-C 12 Alkylene, CHR 4 or CH2; Each Z is independently CH2, CHR 4 , O, NR 4 or NH; Each W 1 independently a water-soluble portion; L 1 , L 2 and L 3 are each independently selected linker moieties; Each E is independently selected from a chromophore, a functional moiety, a substrate, and a binding partner; Each R 4 independently selected from H, alkyl, PEG, a water soluble moiety, a linker moiety, a chromophore, a functional group, a conjugated tag, a carboxylic acid amide, an amine, a carbamate, a carboxylic acid, a carboxylate, a maleimide, an activated ester, an N-hydroxysuccinimidyl group, a hydrazine, a hydrazone, an azide, an alkyne, an alkene, a tetrazine, an aldehyde, and a thiol, or a protected group thereof, Each R 7 Independently selected from H, hydroxyl, C1-C 12 Alkyl, C2-C 12 Olefins, C2-C 12 Alkynes, C3-C 12 Cycloalkyl, C1-C 12 Halogenated alkyl, C1-C 12 Alkoxy, C2-C 18 (Hetero)aryloxy, C2-C 18 (Hetero)arylamino, C2-C 12 Carboxylic acid, C2-C 12 Carboxylic acid esters and C1-C 12 alkoxy, water soluble moiety, PEG moiety, functional group, chemoselective functional group, conjugated tag, attached conjugated tag, linker, sulfonic acid, sulfonate, C1-C 12 Alkyl sulfonates / esters, sulfonamides; Each R 10 independently selected from hydrogen, a linker moiety, an attached reactive group, an attached ionic group, an attached chromophore, an attached water-soluble moiety, a water-soluble moiety, a chromophore, a binding partner, a attached binding partner; Each R 11 independently selected from hydrogen, a linker moiety, an attached reactive group, an attached ionic group, an attached chromophore, an attached water-soluble moiety, a chromophore, a binding partner, and an attached binding partner; Each R 12 and R 13 independently selected from hydrogen, halogen, substituted or unsubstituted C1-C6 alkyl, substituted or unsubstituted C1-C6 heteroalkyl, substituted or unsubstituted C1-C6 alkene, substituted or unsubstituted C3-C 10 cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, and substituted or unsubstituted heteroaryl; or R 12 and R 13 together form an unsubstituted or substituted unsaturated cycloalkyl having 3 to 8 ring members, or a substituted or unsubstituted heterocycloalkyl having 3 to 8 ring members, which is optionally substituted with O; Each K is independently a covalent bond, O, S, P, NR 1 , Se, Te, CR 1 R 2 or CH2; Each f is independently an integer from 0 to 50; Each m is independently 0 or 1; Each n is independently 0, 1, 2, 3 or 4; Each s is independently 1 or 2; and Each t is independently 0, 1, 2 or 3.

12. The polymer dye of claim 11, wherein the monomer of formula (IV) or (V) comprises a structure selected from the group consisting of formula (A1), (A2), (A3), (A4), (A5), (A6), (A7), (A8), (A9), (A10), (A11), (A12), (A13), (A14), (A15), (A16), (A17), (A18) and (A19):

13. A labeled specific binding partner comprising: The fluorescent compound or polymer according to any one of claims 1 to 12; and A specific binding partner is covalently linked to the fluorescent compound or polymer.

14. The labeled specific binding partner of claim 13, wherein the specific binding partner is selected from the group consisting of proteins, peptides, affinity ligands, antibodies, antibody fragments, carbohydrates, lipids, nucleic acids, and aptamers.

15. The labeled specific binding partner of claim 14, wherein the specific binding partner is an antibody.

16. A tandem dye comprising: The fluorescent compound, polymer or labeled specific binding partner according to any one of claims 1 to 15; and An acceptor chromophore or a donor chromophore covalently linked to the fluorescent compound, polymer or labeled specific binding partner.

17. The fluorescent compound, polymer, labeled specific binding partner or tandem dye of any one of claims 1 to 16, wherein R 1 , R 2 , R 3 , R 4 , R 8 , R 9 , R 10 and R 11 At least one of the comprises a water-soluble portion or a linked water-soluble portion.

18. The fluorescent compound, polymer, labeled specific binding partner, or tandem dye of claim 17, wherein the fluorescent compound, polymer, labeled specific binding partner, or tandem dye is water soluble.

19. A method for detecting a target analyte in a sample, the method comprising: Providing a sample suspected of containing the analyte; as well as The sample is contacted with a specific binding partner conjugated to a fluorescent compound, polymer or tandem dye as defined in any one of claims 1 to 18, wherein the specific binding partner is capable of interacting with the target analyte.

20. The method of claim 19, wherein the binding partner is a protein, a peptide, an affinity ligand, an antibody, an antibody fragment, a carbohydrate, a lipid, a nucleic acid, or an aptamer.

21. The method of claim 20, wherein the binding partner is an antibody, optionally wherein: a. The method is configured for flow cytometry; b. The water-soluble fluorescent polymer is bound to the substrate; c. The analyte is a protein expressed on the cell surface; d. The method is configured as an immunoassay; or e. The method further comprises providing an additional binding partner for simultaneously detecting an additional analyte.

22. A kit comprising at least one of the fluorescent compound, polymer, labeled specific binding partner or tandem dye according to any one of claims 1 to 21.

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