Method for inhibiting compositae plant dead body pathogenic bacteria based on salicylic acid

Abstract

The invention provides a method for inhibiting compositae plant dead body pathogenic bacteria based on salicylic acid, and belongs to the technical field of compositae plant prevention and control. The method for inhibiting the compositae plant dead body pathogenic bacteria based on salicylic acid comprises the following steps: soaking seeds, namely soaking the seeds in a potassium permanganate solution for the first time, and then soaking the seeds in a first salicylic acid solution for the second time to obtain pretreated seeds; seed cultivation, wherein the pretreated seeds are cultivated; and salicylic acid cultivation: spraying a second salicylic acid solution on the potted plants with the similar growth vigor, and observing and recording the growth vigor of the potted plants. According to the method, the salicylic acid solution is adopted to pretreat the compositae plant seeds, so that the resistance of compositae plants in the growth process is improved; the growth of the compositae plants is promoted and the resistance of the compositae plants is improved by adopting a salicylic acid post-treatment mode, and meanwhile, the efficacy of salicylic acid is improved by adopting the combined action of jasmonic acid and salicylic acid, so that dead body pathogenic bacteria in the compositae plants are inhibited.

Description

Technical Field

[0001] The invention relates to the technical field of chrysanthemum plant prevention and control, and in particular to a method for inhibiting pathogenic bacteria of dead bodies of chrysanthemum plants based on salicylic acid. Background Art

[0002] There are two main types of plant induced disease resistance, which can be divided into systemic acquired resistance (SAR) and induced systemic resistance (ISR). Systemic acquired resistance is a broad-spectrum resistance response, which is caused by the local infection of plants by living or semi-living trophic pathogens or the treatment of local plant tissues with chemical inducers, resulting in the remote and uninfected parts of the plant being resistant to subsequent multiple pathogens; while induced systemic resistance is caused by some rhizosphere bacteria colonizing the roots of plants, which are not only non-pathogenic but also induce the plant to produce systemic resistance, and can produce resistance to necrotrophic pathogens. The production of SAR is accompanied by the accumulation of salicylic acid (SA).

[0003] Chrysanthemum is a perennial herb and flowering plant of the genus Chrysanthemum in the family Asteraceae. It is native to Asia and northeastern Europe. Most species are native to East Asia, and its diversity center is in China. It is the second most commercially valuable ornamental species in the world, second only to roses. Huai chrysanthemum (Chrysanthemum morifolium Ramat.'Huaihuang'), produced in the "Ancient Huaiqing Mansion" in Jiaozuo, Henan, not only has aesthetic characteristics such as beautiful flowers and pleasant fragrance, but is also one of the famous "Four Huai Medicines". It is a famous traditional medicinal plant that contains a variety of active ingredients and trace elements, such as flavonoids, organic acids, volatile oils, amino acids, carbohydrates, etc., and is believed to have the effects of "improving eyesight, purging the liver, and relieving fever".

[0004] With the development of society and the improvement of living standards, people's demands are getting higher and higher. Asteraceae plants are ornamental, medicinal, health-care, edible, and tea-use varieties, with a wide range of applications and a broad market. However, due to serious diseases, their yields and quality have decreased, which has seriously restricted the production and sales of Asteraceae plants. Therefore, it is urgent to solve the problem of diseases and pests.

[0005] Based on this, providing a method for inhibiting pathogens of dead bodies of Asteraceae plants based on salicylic acid has important practical significance. Summary of the invention

[0006] The purpose of the present invention is to provide a method for inhibiting pathogens of dead bodies of Asteraceae plants based on salicylic acid, aiming to solve the technical problem in the prior art that diseases and insect pests of Asteraceae plants seriously affect their quality and yield.

[0007] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0008] The present invention provides a method for inhibiting pathogenic bacteria of dead bodies of Asteraceae plants based on salicylic acid, comprising the following steps:

[0009] Seed soaking: taking a number of seeds, placing the seeds in a potassium permanganate solution for a first soaking, and after the soaking is completed, using a first salicylic acid solution for a second soaking to obtain pretreated seeds;

[0010] Seed cultivation: cultivating the pretreated seeds to obtain potted plants with similar growth potential;

[0011] Salicylic acid cultivation: spraying the second salicylic acid solution on the potted plants with similar growth potential, and observing and recording their growth potential;

[0012] Wherein, the seeds are seeds of Asteraceae plants.

[0013] Furthermore, the concentration of the potassium permanganate solution is 5% to 10%.

[0014] Furthermore, the first soaking time is 20 to 30 minutes.

[0015] Furthermore, the concentration of the first salicylic acid solution is 300-400 mg / L.

[0016] Furthermore, the second salicylic acid solution includes 500-800 mg / L of salicylic acid solution and 500-800 mg / L of jasmonic acid solution.

[0017] Furthermore, the second soaking time is 24 to 48 hours.

[0018] Furthermore, the spraying is performed every 7 to 10 days, and the number of spraying times is 3 to 5 times.

[0019] The present invention also provides the application of the method for inhibiting pathogenic bacteria of dead bodies of Asteraceae plants based on salicylic acid described in the above technical scheme in the prevention and control of Asteraceae plants.

[0020] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0021] The present invention adopts a method of pretreating the seeds of Asteraceae plants with a salicylic acid solution to improve the resistance of Asteraceae plants during their growth process; a salicylic acid post-treatment method is also adopted to promote the growth of Asteraceae plants and improve their resistance; jasmonic acid and salicylic acid are used to work together to improve the efficacy of salicylic acid, thereby inhibiting dead pathogens in Asteraceae plants. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The present invention is a flow chart of the method for inhibiting pathogens of dead bodies of Asteraceae plants based on salicylic acid. DETAILED DESCRIPTION

[0023] The present invention provides a method for inhibiting pathogenic bacteria of dead bodies of Asteraceae plants based on salicylic acid, comprising the following steps:

[0024] Seed soaking: taking a number of seeds, placing the seeds in a potassium permanganate solution for a first soaking, and after the soaking is completed, using a first salicylic acid solution for a second soaking to obtain pretreated seeds;

[0025] Seed cultivation: cultivating the pretreated seeds to obtain potted plants with similar growth potential;

[0026] Salicylic acid cultivation: spraying the second salicylic acid solution on the potted plants with similar growth potential, and observing and recording their growth potential;

[0027] Wherein, the seeds are seeds of Asteraceae plants.

[0028] Seed soaking: taking a number of seeds, placing the seeds in a potassium permanganate solution for a first soaking, and after the soaking is completed, using a first salicylic acid solution for a second soaking to obtain pretreated seeds;

[0029] The invention adopts potassium permanganate solution to disinfect the seeds to be treated, and kills bacteria and viruses that may exist in the seeds.

[0030] In the present invention, the concentration of the potassium permanganate solution is preferably 5% to 10%, more preferably 5% to 8%, and more preferably 5% to 6%.

[0031] In the present invention, the first immersion time is preferably 20 to 30 minutes, more preferably 20 to 28 minutes, and even more preferably 20 to 25 minutes.

[0032] In the present invention, the concentration of the first salicylic acid solution is preferably 300 to 400 mg / L, more preferably 300 to 380 mg / L, and even more preferably 300 to 350 mg / L.

[0033] In the present invention, the second salicylic acid solution preferably includes 500-800 mg / L salicylic acid solution and 500-800 mg / L jasmonic acid solution, further preferably includes 500-700 mg / L salicylic acid solution and 500-700 mg / L jasmonic acid solution, and more preferably includes 500-600 mg / L salicylic acid solution and 500-600 mg / L jasmonic acid solution.

[0034] In the present invention, the second immersion time is preferably 24 to 48 hours, and more preferably 24 to 36 hours.

[0035] Seed cultivation: cultivating the pretreated seeds to obtain potted plants with similar growth potential;

[0036] The present invention places the pretreated seeds under a constant temperature of 18-20° C. and cultivates them for 40-60 days, during which phosphorus and potassium fertilizers are applied every ten days.

[0037] Salicylic acid cultivation: spraying the second salicylic acid solution on the potted plants with similar growth potential, and observing and recording their growth potential;

[0038] In the present invention, the spraying is preferably performed once every 7 to 10 days, with the number of spraying being 3 to 5 times, and more preferably once every 7 to 8 days, with the number of spraying being 3 to 4 times.

[0039] The present invention also provides the application of the method for inhibiting pathogenic bacteria of dead bodies of Asteraceae plants based on salicylic acid described in the above technical scheme in the prevention and control of Asteraceae plants.

[0040] In the present invention, unless otherwise specified, the required raw materials for preparation are all commercially available products well known to those skilled in the art.

[0041] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0042] Example 1

[0043] (1) Seed soaking: potassium permanganate is dissolved in water to obtain a potassium permanganate solution with a concentration of 5%, and salicylic acid solution is prepared to obtain a salicylic acid solution with a concentration of 300 mg / L. Chrysanthemum seeds are completely immersed in the potassium permanganate solution for disinfection for 20 minutes. After the soaking, the seeds are soaked in the prepared salicylic acid solution for 24 hours, and then air-dried to obtain pretreated seeds;

[0044] (2) Seed cultivation: the pretreated seeds are placed in a constant temperature cultivation condition of 18-20° C. for 40 days, during which phosphorus and potassium fertilizers are applied every ten days to obtain potted plants with similar growth;

[0045] (3) Salicylic acid cultivation: salicylic acid was used to prepare salicylic acid solution, and then jasmonic acid solution was added to make the concentration of salicylic acid in the final solution be 500 mg / L and the concentration of jasmonic acid be 500 mg / L. The prepared salicylic acid solution was sprayed on potted plants with similar growth, once every 7 days, for 3 consecutive times, and their growth was observed and recorded.

[0046] Example 2

[0047] (1) Seed soaking: potassium permanganate is dissolved in water to obtain a potassium permanganate solution with a concentration of 10%, and salicylic acid solution is prepared at the same time to obtain a salicylic acid solution with a concentration of 350 mg / L. Chrysanthemum seeds are completely immersed in the potassium permanganate solution for disinfection for 25 minutes. After the soaking, the seeds are soaked in the prepared salicylic acid solution for 36 hours, and then air-dried to obtain pretreated seeds;

[0048] (2) Seed cultivation: the pretreated seeds are placed in a constant temperature cultivation condition of 18-20° C. for 50 days, during which phosphorus and potassium fertilizers are applied every ten days to obtain potted plants with similar growth;

[0049] (3) Salicylic acid cultivation: salicylic acid was used to prepare salicylic acid solution, and then jasmonic acid solution was added to make the concentration of salicylic acid in the final solution be 600 mg / L and the concentration of jasmonic acid be 600 mg / L. The prepared salicylic acid solution was sprayed on potted plants with similar growth, once every 8 days, for 4 consecutive times, and their growth was observed and recorded.

[0050] Example 3

[0051] (1) Seed soaking: potassium permanganate is dissolved in water to obtain a potassium permanganate solution with a concentration of 8%, and salicylic acid solution is prepared at the same time to obtain a salicylic acid solution with a concentration of 380 mg / L. Chrysanthemum seeds are completely immersed in the potassium permanganate solution for disinfection for 20 minutes. After the soaking, the seeds are soaked in the prepared salicylic acid solution for 48 hours, and then air-dried to obtain pretreated seeds;

[0052] (2) Seed cultivation: the pretreated seeds are placed in a constant temperature cultivation condition of 18-20° C. for 60 days, during which phosphorus and potassium fertilizers are applied every ten days to obtain potted plants with similar growth;

[0053] (3) Salicylic acid cultivation: salicylic acid was used to prepare salicylic acid solution, and then jasmonic acid solution was added to make the concentration of salicylic acid in the final solution be 800 mg / L and the concentration of jasmonic acid be 800 mg / L. The prepared salicylic acid solution was sprayed on potted plants with similar growth, once every 10 days, for 3 consecutive times, and their growth was observed and recorded.

[0054] A blank control group was set up, in which the seeds of the Asteraceae plants were not subjected to any operation, and ordinary cultivation was performed, and their growth was observed and recorded; based on the observation and recording of Examples 1 to 3 and the blank control group, it was found that the presence rate of dead pathogens in the potted plants obtained in Examples 1 to 3 was greatly reduced compared with the blank control group. It can be seen that the method of inhibiting dead pathogens of Asteraceae plants based on salicylic acid according to the present invention can significantly reduce dead pathogens in Asteraceae plants.

[0055] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for inhibiting pathogens of dead plants of Asteraceae based on salicylic acid, characterized in that: The following steps are involved: Seed soaking: taking a number of seeds, placing the seeds in a potassium permanganate solution for a first soaking, and after the soaking is completed, using a first salicylic acid solution for a second soaking to obtain pretreated seeds; Seed cultivation: cultivating the pretreated seeds to obtain potted plants with similar growth potential; Salicylic acid cultivation: spraying the second salicylic acid solution on the potted plants with similar growth potential, and observing and recording their growth potential; Wherein, the seeds are seeds of Asteraceae plants.

2. The method for inhibiting dead pathogens of Asteraceae plants based on salicylic acid according to claim 1, characterized in that: The concentration of the potassium permanganate solution is 5% to 10%.

3. The method for inhibiting dead pathogens of Asteraceae plants based on salicylic acid according to claim 1, characterized in that: The first soaking time is 20 to 30 minutes.

4. The method for inhibiting pathogens of dead Compositae plants based on salicylic acid according to claim 1, characterized in that: The concentration of the first salicylic acid solution is 300-400 mg / L.

5. The method for inhibiting dead pathogens of Asteraceae plants based on salicylic acid according to claim 1, characterized in that: The second salicylic acid solution includes 500-800 mg / L of salicylic acid solution and 500-800 mg / L of jasmonic acid solution.

6. The method for inhibiting dead pathogens of Asteraceae plants based on salicylic acid according to claim 1, characterized in that: The second soaking time is 24 to 48 hours.

7. The method for inhibiting dead pathogens of Asteraceae plants based on salicylic acid according to claim 1, characterized in that: The spraying is performed once every 7 to 10 days, and the number of spraying times is 3 to 5 times.

8. Use of the method for inhibiting pathogens of dead bodies of Asteraceae plants based on salicylic acid according to any one of claims 1 to 7 in the control of Asteraceae plants.

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