Method for increasing anthocyanin content of triticale grains

Abstract

The invention relates to the technical field of crop cultivation, and particularly discloses a method for increasing the anthocyanin content of triticale grains, which comprises the following steps: respectively spraying 80-100 times of leaf fertilizer diluent once at the elongation stage and filling stage of triticale growth, and spraying 6-10kg / mu each time; the formula of the foliar fertilizer is as follows: 100 mg to 150 mg of mannose, 100 mg to 150 mg of fructose, 130 mg to 160 mg of mannitol, 160 mg to 200 mg of betaine, 180 mg to 200 mg of alginic acid, 180 mg to 200 mg of fucoidin, 300 mg to 350 mg of amino acid and 1000 g of water. According to the method, the content of anthocyanin in triticale grains can be remarkably increased by spraying 80-100 times of foliar fertilizer diluent prepared by the method in the elongation stage and the pustulation stage of the triticale.

Description

Technical Field

[0001] The invention relates to the technical field of crop cultivation, and in particular to a method for increasing the anthocyanin content of black wheat grains. Background Art

[0002] Wheat is a general term for plants of the genus Triticeae. The representative species is common wheat (scientific name: Triticum aestivum L.), which is a plant of the Poaceae family and a cereal crop widely grown throughout the world. The caryopsis of wheat is one of the staple foods of mankind. After being ground into flour, it can be used to make bread, steamed buns, biscuits, noodles and other foods. After fermentation, it can be made into beer, alcohol, liquor or biomass fuel.

[0003] Colorful wheat is a valuable resource, rich in anthocyanins and minerals, thus providing additional nutritional value to a healthy human diet. In addition to genetic factors, abiotic environmental factors such as light and heat characteristics and fertilizer levels can also affect anthocyanin metabolism. There are many factors that affect the anthocyanin content of black wheat, such as variety characteristics, climate, soil nutrition, cultivation practices, etc. In particular, nitrogen fertilizer is an important agricultural technology that can be used to improve crop quality and yield, also because nitrogen is an essential element for most biological compounds.

[0004] The husk of common wheat usually presents a golden luster, while black wheat is characterized by its dark brown or black husk, which is a color derived from its rich anthocyanins. Compared with common wheat, wheat rich in anthocyanins (such as purple wheat and blue wheat) shows unique advantages in nutritional value. They contain higher dietary fiber, which helps to improve the function of the digestive system. At the same time, these wheats are also richer in minerals, especially trace elements such as iron, zinc, and magnesium. More importantly, they are rich in antioxidants, such as anthocyanins and flavonoids, which can effectively resist free radicals, enhance immunity, and are also of great benefit to cardiovascular health. Anthocyanins have strong antioxidant capacity, which is dozens of times that of vitamin E. It can effectively remove free radicals in the body and play multiple roles such as anti-aging and anti-cancer. It also has a significant protective effect on cardiovascular and cerebrovascular health. Different varieties of wheat have different anthocyanin content.

[0005] Although black wheat is a cereal variety rich in anthocyanins and has multiple health benefits, the cultivation of black wheat mainly includes key steps such as land cultivation, sowing, field management and harvesting. Although fertilization in field management is conducive to increasing yield, improper fertilization management will affect the accumulation of anthocyanins and cause a decrease in anthocyanin content. Therefore, it is necessary to provide a method for increasing the anthocyanin content of black wheat grains. Summary of the invention

[0006] In order to increase the anthocyanin content of black wheat grains, the present invention provides a method for increasing the anthocyanin content of black wheat grains. The present invention can significantly increase the anthocyanin content of black wheat grains by spraying the 80-100 times diluted foliar fertilizer prepared by the present invention once during the jointing stage and the filling stage of wheat.

[0007] The present invention provides a method for increasing the anthocyanin content of black wheat grains. During the jointing stage and the filling stage of the black wheat, 80-100 times diluted foliar fertilizer is sprayed once respectively, and the spraying amount each time is 6kg / mu to 10kg / mu;

[0008] The foliar fertilizer formula is: 100mg~150mg mannose, 100mg~150mg fructose, 130mg~160mg mannitol, 160mg~200mg betaine, 180mg~200mg alginic acid, 180mg~200mg fucoidan, 300mg~350mg amino acid, 1000g water;

[0009] The amino acids are composed of phenylalanine, glycine, glutamic acid, aspartic acid, proline, arginine and valine in a mass ratio of 45-49:4:20-26:6:6-10:3:2.

[0010] The invention sprays the 80-100 times diluted foliar fertilizer prepared by the invention once in the jointing stage and the filling stage of wheat respectively, thereby up-regulating the expression of anthocyanin synthesis-related genes and promoting anthocyanin synthesis, thereby significantly increasing the anthocyanin content of black wheat grains.

[0011] Furthermore, the foliar fertilizer formula is: 100 mg mannose, 150 mg fructose, 130 mg mannitol, 200 mg betaine, 200 mg alginic acid, 180 mg fucoidan, 300 mg amino acids, and 1000 g water.

[0012] Furthermore, the foliar fertilizer formula is: 150 mg mannose, 100 mg fructose, 160 mg mannitol, 160 mg betaine, 180 mg alginic acid, 200 mg fucoidan, 350 mg amino acids, and 1000 g water.

[0013] Furthermore, the method for increasing the anthocyanin content of black wheat grains also includes land preparation, sowing and harvesting.

[0014] Furthermore, the land preparation process is: applying corn bran fermented organic fertilizer as base fertilizer, and the application amount of corn bran fermented organic fertilizer is: 25kg / mu to 30kg / mu.

[0015] Furthermore, the preparation process of corn bran fermented organic fertilizer is as follows: corn stalks are crushed and evenly mixed with urea, cake fertilizer, diammonium phosphate and fermentation agent in a mass ratio of 90-100:15-20:4-6:3, water is added to adjust the moisture content to 65%-75%, and then the corn bran fermented organic fertilizer is obtained after composting and fermenting at 18°C-20°C for 18-20 days.

[0016] Furthermore, before sowing, the seeds are mixed with 1.5% to 2% tebuconazole by mass percentage, and the sowing amount is 200 kg ha -1 ~225kg·ha -1 , row spacing is 15cm~20cm, and sowing depth is 4±1cm.

[0017] Furthermore, the black wheat is Linhei 199.

[0018] The present invention also provides a foliar fertilizer for increasing the anthocyanin content of black wheat grains, the foliar fertilizer formula is: 100mg-150mg mannose, 100mg-150mg fructose, 130mg-160mg mannitol, 160mg-200mg betaine, 180mg-200mg alginic acid, 180mg-200mg fucoidan, 300mg-350mg amino acids, 1000g water;

[0019] The amino acids are composed of phenylalanine, glycine, glutamic acid, aspartic acid, proline, arginine and valine in a mass ratio of 45-49:4:20-26:6:6-10:3:2.

[0020] The present invention also provides the use of the foliar fertilizer in improving the anthocyanin content of black wheat grains. By spraying the foliar fertilizer dilution during the jointing stage and the filling stage of the black wheat, the expression of anthocyanin synthesis-related genes is upregulated, anthocyanin synthesis is promoted, and the anthocyanin content of the black wheat grains is increased.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The invention sprays the 80-100 times diluted foliar fertilizer prepared by the invention once during the jointing stage and the grain filling stage of wheat, thereby up-regulating the expression of anthocyanin synthesis-related genes, promoting anthocyanin synthesis, and thus significantly increasing the anthocyanin content of black wheat grains.

[0023] The 80-100 times foliar fertilizer dilution prepared by the present invention can significantly increase the anthocyanin content of black wheat grains. The anthocyanin content in the black wheat grains harvested by spraying the foliar fertilizer is increased by 43.21 μg / g compared with that without spraying the foliar fertilizer. By diluting the foliar fertilizer by different times for spraying, it is found that when the dilution multiple is too large (200-300 times), compared with the 80-100 times foliar fertilizer dilution, the anthocyanin content of the black wheat grains is significantly reduced, and the 200-fold foliar fertilizer dilution and the 300-fold foliar fertilizer dilution respectively reduce the anthocyanin content by 22.44 μg / g and 29.9 μg / g compared with the 100-fold foliar fertilizer dilution.

[0024] After spraying the foliar fertilizer prepared by the present invention, no additional nitrogen fertilizer can be added. The additional nitrogen fertilizer seriously inhibits the synthesis of anthocyanins. The higher the amount of nitrogen fertilizer applied in the topdressing, the lower the anthocyanin content, indicating that the amount of nitrogen fertilizer applied is inversely proportional to the anthocyanin content.

[0025] The 80-100 times dilution of foliar fertilizer significantly promoted the expression of F3'H and ANS genes. The 50 times dilution of foliar fertilizer caused seedling burn due to the high concentration, which disturbed the anthocyanin synthesis pathway and significantly downregulated the expression of F3'H and ANS genes.

[0026] The addition of nitrogen fertilizer significantly down-regulated the expression of F3'H and ANS genes, and the higher the nitrogen fertilizer application rate, the more significant the down-regulation of F3'H and ANS gene expression was. In particular, the effect on ANS gene was higher than that on F3'H. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0028] Figure 1 This is the effect of foliar fertilizer dilution multiples on anthocyanin synthesis-related genes.

[0029] Figure 2 To investigate the effect of additional nitrogen fertilizer on genes related to anthocyanin synthesis. DETAILED DESCRIPTION

[0030] The specific embodiments of the present invention are described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention. The experimental methods described in the embodiments of the present invention are conventional methods unless otherwise specified, and the materials, reagents, etc. used in the following embodiments can be obtained from commercial channels unless otherwise specified.

[0031] The experimental area of ​​the present invention is located in the wheat cultivation area of ​​Hongbao Village, Yaodu District, Linfen City, and is divided into several 50m 2 The following experiment was carried out on a sample plot area of ​​(5m×10m).

[0032] Example 1: A method for increasing the anthocyanin content of black wheat grains.

[0033] 1. Experimental Materials and Methods

[0034] 1. Experimental seed materials

[0035] Wheat variety: Linhei 199.

[0036] 2. Fertilizer

[0037] Nitrogen, phosphorus and potassium compound fertilizer (15-15-15, Shandong Kemei Chemical Trading Co., Ltd., Aonongfeng).

[0038] Foliar fertilizer: 100mg mannose, 150mg fructose, 130mg mannitol, 200mg betaine, 200mg alginic acid, 180mg fucoidan, 300mg amino acid, 1000g water. Mix all the ingredients and use.

[0039] The types and contents of amino acids in the amino acids are shown in Table 1.

[0040] Table 1 Amino acid types

[0041] Ingredients content(%) Phenylalanine 49 Glycine 4 Glutamate 26 Aspartic acid 6 Proline 10 Arginine 3 Valine 2

[0042] Corn bran fermented organic fertilizer: crush corn stalks and mix them evenly with urea, cake fertilizer, diammonium phosphate and fermentation agent in a mass ratio of 100:20:6:3, add water to adjust the moisture content to 70%, and then compost and ferment at 18℃ for 20 days to obtain corn bran fermented organic fertilizer. During the fermentation process, the internal temperature of the compost is controlled at 60℃~65℃. If the temperature is too high, turn the compost, add water appropriately and mix the raw materials to control the temperature rise.

[0043] 3. Land preparation

[0044] Apply corn bran fermented organic fertilizer as base fertilizer, apply 30kg corn bran fermented organic fertilizer per mu, and till the soil to 25cm.

[0045] 4. Planting seeds

[0046] Select black wheat variety Linhei 199 with full grains for sowing, mix 50g of 2% tebuconazole seed dressing with 50kg of wheat seeds before sowing, stir evenly, and then seal the wheat seeds with plastic bags for 30 minutes before sowing;

[0047] Sowing: Sowing was carried out from October 8 to October 15, with a sowing rate of 225 kg / ha -1 , row spacing is 20cm and sowing depth is 4cm.

[0048] 5. Fertilization management

[0049] In the jointing stage and the grain filling stage of wheat growth, the foliar fertilizer is diluted 100 times with water to obtain a 100-fold foliar fertilizer dilution solution, which is sprayed on the leaves, and the spraying amount in the jointing stage and the grain filling stage is 8kg / mu. The foliar fertilizer in the present invention can not only provide basic nutrients for wheat, but also provide raw materials for anthocyanin synthesis through the leaves, and the expression of anthocyanin synthase ANS gene can be promoted by spraying the foliar fertilizer on the leaves, thereby promoting the synthesis of anthocyanin in wheat seeds.

[0050] 6. Harvest: Harvest black wheat grains after the wheat matures.

[0051] Example 2: A method for increasing the anthocyanin content of black wheat grains.

[0052] It is basically the same as Example 1, except that: the components of the foliar fertilizer are: 150 mg mannose, 100 mg fructose, 160 mg mannitol, 160 mg betaine, 180 mg alginic acid, 200 mg fucoidan, 350 mg amino acid, and 1000 g water.

[0053] Example 3: A method for increasing the anthocyanin content in black wheat grains.

[0054] According to the experimental steps of Example 1, land preparation and sowing were carried out. During the fertilization management, the foliar fertilizer was diluted 80 times to obtain an 80-fold foliar fertilizer dilution solution.

[0055] Comparative Example 1: A method for improving black wheat planting.

[0056] Land preparation and sowing were carried out according to the experimental steps of Example 1, and no fertilization was performed during the fertilization management process, which served as a blank control.

[0057] Comparative Example 2: A method for improving black wheat planting.

[0058] The land was prepared and sown according to the experimental steps of Example 1. During the fertilization management, foliar fertilizer was used and nitrogen-phosphorus-potassium compound fertilizer (nitrogen fertilizer) was used for topdressing 10 days later. The fertilizer application rate was 50 kg ha -1.

[0059] Comparative Example 3: A method for improving black wheat planting.

[0060] The soil was prepared and sown according to the experimental steps of Example 1. During the fertilization management, foliar fertilizer was used and then nitrogen-phosphorus-potassium compound fertilizer (nitrogen fertilizer) was used for topdressing 10 days later. The fertilizer application rate was 150 kg ha -1 .

[0061] Comparative Example 4: A method for improving black wheat planting.

[0062] The soil was prepared and sown according to the experimental steps of Example 1. During the fertilization management, foliar fertilizer was used and nitrogen-phosphorus-potassium compound fertilizer (nitrogen fertilizer) was used for topdressing 10 days later. The fertilizer application rate was 225 kg ha -1 .

[0063] Comparative Example 5: A method for improving black wheat planting.

[0064] Land preparation and sowing were carried out according to the experimental steps of Example 1. During the fertilization management, the foliar fertilizer was diluted 200 times to obtain a 200-fold foliar fertilizer dilution solution.

[0065] Comparative Example 6: A method for improving black wheat planting.

[0066] Land preparation and sowing were carried out according to the experimental steps of Example 1. During the fertilization management, the foliar fertilizer was diluted 300 times to obtain a 300-fold foliar fertilizer dilution solution.

[0067] Comparative Example 7: A method for improving black wheat planting.

[0068] According to the experimental steps of Example 1, land preparation and sowing were carried out. During the fertilization management, the foliar fertilizer was diluted 50 times to obtain a 50-fold foliar fertilizer dilution solution.

[0069] 2. According to the above examples and comparative examples, black wheat was planted, and the anthocyanin content and protein content of the mature grains were measured. The experimental results are shown in Table 2.

[0070] Table 2 Anthocyanin content and protein content of mature grains

[0071] Experimental design Grouping characteristics Protein content (g / 100g) Anthocyanin content (μg / g) Example 1 100 times diluted foliar fertilizer spray 23.57 96.35 Example 2 Different content of foliar fertilizer components 22.31 95.81 Example 3 80 times foliar fertilizer dilution spray 23.35 98.76 Comparative Example 1 Blank control (no foliar fertilizer) 15.64 53.14 Comparative Example 2 <![CDATA[Topdressing nitrogen fertilizer at 10 kg·ha -1 (low nitrogen)]]> 15.94 45.02 Comparative Example 3 <![CDATA[Topdressing nitrogen fertilizer at 150 kg·ha -1 (medium nitrogen)]]> 18.87 23.73 Comparative Example 4 <![CDATA[Topdressing nitrogen fertilizer at 225 kg·ha -1 (high nitrogen)]]> 22.31 11.11 Comparative Example 5 200 times diluted foliar fertilizer spray 20.23 73.91 Comparative Example 6 300 times diluted foliar fertilizer spray 19.83 66.45 Comparative Example 7 Spray 50 times diluted foliar fertilizer 17.88 60.32

[0072] As shown in Table 2, the present invention can significantly increase the anthocyanin content of black wheat grains by spraying the 80-100 times foliar fertilizer dilution prepared by the present invention once in the jointing stage and the filling stage of wheat. As can be seen from Example 1 and Comparative Example 1, the anthocyanin content in the black wheat grains harvested by spraying foliar fertilizer is increased by 43.21 μg / g compared with that without spraying foliar fertilizer. By diluting the foliar fertilizer by different multiples for spraying, it is found that when the dilution multiple is too large (200-300 times), the anthocyanin content of black wheat grains is significantly reduced compared with the 80-100 times foliar fertilizer dilution, and the 200 times foliar fertilizer dilution and the 300 times foliar fertilizer dilution make the anthocyanin content compared with the 100 times foliar fertilizer dilution respectively reduced by 22.44 μg / g and 29.9 μg / g. In Comparative Example 7, the foliar fertilizer concentration was too high, which caused seedling burn and seriously affected the growth and development of wheat, thereby affecting the synthesis of anthocyanins. The spraying of 50-fold diluted foliar fertilizer significantly reduced the anthocyanin content.

[0073] By comparing Example 1 with Comparative Examples 2 to 4, it can be seen that after spraying the foliar fertilizer prepared by the present invention, no additional nitrogen fertilizer can be added. The additional nitrogen fertilizer seriously inhibits the synthesis of anthocyanins. The higher the amount of nitrogen fertilizer applied in the topdressing, the lower the anthocyanin content, indicating that the amount of nitrogen fertilizer applied is inversely proportional to the anthocyanin content.

[0074] 3. Effects of different treatments on the expression of key genes in the anthocyanin synthesis pathway

[0075] 1. Extraction of total RNA from black wheat grains

[0076] The total RNA of the black wheat grains harvested in the above examples and comparative examples was extracted using the Plant Total RNA Extraction Kit of Biofit Company. The RNA samples were reverse transcribed into cDNA using the Primer-Script TM RT reagent Kit of TaKaRa Company and stored at -20°C for later use.

[0077] 2. Quantitative primers

[0078] The primers for the key genes F3'H and ANS in the anthocyanin biosynthesis pathway were synthesized by Sangon Biotechnology (Shanghai) Co., Ltd., and the internal reference gene was Actin. The reaction of each sample to be tested was repeated three times, and the cDNA template blank was used as the blank control. The primer information is shown in Table 3.

[0079] Table 3 Specific primers for fluorescence quantitative PCR

[0080]

[0081] Reaction system 25μl: Green Premix Ex TaqT TM(2x) 12.5μl; PCR primer-F (10μM) 0.5μl; PCR primer-R (10μM) 0.5μl; RNA-Free H2O 9.5μl; cDNA template 2.0μl.

[0082] The reaction procedure was two-step method: pre-denaturation at 95°C, 10 sec; denaturation at 95°C, 5 sec; annealing at 60°C, 20 sec; a total of 40 cycles.

[0083] Use 2- ΔΔCT The algorithm was used to calculate the relative expression of the gene to be tested. Three biological replicates were performed for each sample. Excel 2007 and SPSS 21.0 software were used for data calculation and processing for statistical and correlation analysis and plotting. The least significant difference (LSD) method was used to test the significance level of the experimental data (P < 0.05).

[0084] 3. Experimental results

[0085] The present invention studies the effect of the foliar fertilizer of the present invention on anthocyanin synthesis by detecting the key genes F3'H and ANS of the anthocyanin synthesis pathway of black wheat seeds harvested from various embodiments and comparative examples.

[0086] The effect of foliar fertilizer dilution multiple on anthocyanin synthesis-related genes was studied by detecting the expression of F3'H and ANS genes in black wheat seeds harvested from Example 1, Example 3 and Comparative Examples 5 to 7.

[0087] The results are as follows Figure 1 As shown, it is consistent with the trend of anthocyanin content in Table 2. 80-100 times foliar fertilizer dilution significantly promoted the expression of F3'H and ANS genes. However, the expression of F3'H and ANS genes in Comparative Examples 5 and 6 was downregulated. The 50-fold foliar fertilizer dilution in Comparative Example 7 caused seedling burn due to excessive concentration, which disturbed the anthocyanin synthesis pathway and significantly downregulated the expression of F3'H and ANS genes.

[0088] The effect of additional nitrogen fertilizer on anthocyanin synthesis-related genes was studied by detecting the expression of F3'H and ANS genes in black wheat seeds harvested from Comparative Examples 1 to 4.

[0089] The results are as follows Figure 2 As shown, additional nitrogen fertilizer significantly down-regulated the expression of F3'H and ANS genes, and the higher the nitrogen fertilizer application rate, the more significant the down-regulation of F3'H and ANS gene expression was. In particular, the effect on ANS gene was greater than that on F3'H.

[0090] In summary, the present invention significantly increases the anthocyanin content of black wheat grains by spraying the 80-100 times diluted foliar fertilizer prepared by the present invention on the leaves during the jointing stage and the filling stage of wheat.

[0091] It should be noted that when the present invention involves a numerical range, it should be understood that the two endpoints of each numerical range and any numerical value between the two endpoints can be selected. Since the steps and methods used are the same as those in the embodiment, in order to avoid redundancy, the present invention describes a preferred embodiment.

[0092] Although preferred embodiments of the present invention have been described, additional changes and modifications may occur to these embodiments once those skilled in the art are aware of the basic inventive concepts.

[0093] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A method for increasing the anthocyanin content of black wheat grains, characterized in that: During the jointing stage and grain filling stage of black wheat, spray 80-100 times diluted foliar fertilizer once, with the spraying amount of 6kg / mu to 10kg / mu each time; The foliar fertilizer formula is: 100mg~150mg mannose, 100mg~150mg fructose, 130mg~160mg mannitol, 160mg~200mg betaine, 180mg~200mg alginic acid, 180mg~200mg fucoidan, 300mg~350mg amino acid, 1000g water; The amino acids are composed of phenylalanine, glycine, glutamic acid, aspartic acid, proline, arginine and valine in a mass ratio of 45-49:4:20-26:6:6-10:3:

2.

2. The method for increasing anthocyanin content in black wheat grains according to claim 1, characterized in that: The foliar fertilizer formula is: 100 mg mannose, 150 mg fructose, 130 mg mannitol, 200 mg betaine, 200 mg alginic acid, 180 mg fucoidan, 300 mg amino acid, and 1000 g water.

3. The method for increasing anthocyanin content in black wheat grains according to claim 1, characterized in that: The foliar fertilizer formula is: 150 mg mannose, 100 mg fructose, 160 mg mannitol, 160 mg betaine, 180 mg alginic acid, 200 mg fucoidan, 350 mg amino acid, and 1000 g water.

4. The method for increasing anthocyanin content in black wheat grains according to claim 1, characterized in that: It also includes land preparation, sowing and harvesting.

5. The method for increasing anthocyanin content in black wheat grains according to claim 1, characterized in that: The land preparation process is: apply corn bran fermented organic fertilizer as base fertilizer, and the application amount of corn bran fermented organic fertilizer is: 25kg / mu ~ 30kg / mu.

6. The method for increasing anthocyanin content in black wheat grains according to claim 5, characterized in that: The preparation process of corn bran fermented organic fertilizer is as follows: corn stalks are crushed and evenly mixed with urea, cake fertilizer, diammonium phosphate and fermentation agent in a mass ratio of 90-100:15-20:4-6:3, water is added to adjust the moisture content to 65%-75%, and then the corn bran fermented organic fertilizer is obtained after composting and fermenting at 18°C-20°C for 18-20 days.

7. The method for increasing anthocyanin content in black wheat grains according to claim 4, characterized in that: Before sowing, mix the seeds with 1.5% to 2% tebuconazole by mass percentage, and the sowing amount is 200 kg ha -1 ~225kg·ha -1 , row spacing is 15cm~20cm, and sowing depth is 4±1cm.

8. The method for increasing anthocyanin content in black wheat grains according to claim 4, characterized in that: The black wheat is Linhei 199.

9. A foliar fertilizer for increasing the anthocyanin content of black wheat grains, characterized in that: The foliar fertilizer is the foliar fertilizer in claim 1.

10. The use of the foliar fertilizer according to claim 9 in increasing the anthocyanin content of black wheat grains, characterized in that: By spraying diluted foliar fertilizer during the jointing and filling stages of black wheat growth, the expression of anthocyanin synthesis-related genes can be upregulated, anthocyanin synthesis can be promoted, and the anthocyanin content of black wheat grains can be increased.

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