Application of brassinolide in prevention of fruit drop of waxberries

Abstract

The invention provides application of brassinolide in prevention of fruit drop of waxberries, and belongs to the technical field of fruit drop prevention medicaments. According to the method, in the core hardening stage, the white turning stage and the red turning stage of waxberry plum fruits, the white-bark water solution with the concentration of 0.8-1.2 mg / L is sprayed to leaves and petioles of waxberry plants, waxberry fruit dropping can be effectively prevented, tests show that after the white-bark water solution is sprayed, the waxberry fruit dropping rate is reduced to 17.73% from 43.99%, and the effect is better than that of p-chlorophenylalanine commonly used in the market. In addition, the brachycarpus oldenlandia alcohol is a natural plant metabolite, is green, safe and non-toxic, provides a new thought for preventing fruit dropping of the waxberries, and is suitable for large-scale popularization and application.

Description

Technical Field

[0001] The invention relates to the technical field of anti-fruit drop agents, and in particular to application of picathanol in preventing bayberry fruit drop. Background Art

[0002] Bayberry (Morella rubra Sieb.et.Zucc.), also known as Shengshengmei and Baidimei, is a small tree or shrub of the genus Myrica in the Myricaceae family. Bayberry is sweet and sour, with a strong flavor, and is rich in sugar, acid, vitamins, anthocyanins and other antioxidant substances, with high nutritional value. Bayberry can be eaten directly, processed into dried bayberry, sauce, candied fruit, etc., and can also be used to make wine. It has the functions of quenching thirst, promoting body fluid, and aiding digestion. Bayberry is widely planted in Zhejiang and has many varieties. In particular, "Dingao Plum" (Myrica rubracv.Dingao) is a national geographical indication product and one of the four major bayberry varieties in Zhejiang Province. It enjoys the reputation of "red plate and green pedicle". However, the ripening period of bayberry in Zhejiang is mainly concentrated in mid-to-late June, which is the plum rain season in the lower reaches of the Yangtze River. The heavy rain and high humidity can easily cause the bayberry to fall before picking, which greatly reduces the quality of the fruit and causes serious economic losses, thus affecting the long-term development of the bayberry industry.

[0003] At present, chemical control is still the main measure to prevent fruit drop in the bayberry industry. For example, 2,4-D sodium salt (also known as 2,4-dichlorophenoxyacetic acid sodium salt) is a selective systemic hormone-transmitting chemical agent, mainly used as a herbicide and plant growth regulator, and is a commonly used pesticide to prevent bayberry fruit drop. Although the residue of 2,4-D sodium salt poses a safety hazard, some farmers still use low-concentration 2,4-D sodium salt as a bayberry fruit drop prevention agent to reduce the yield loss caused by pre-harvest fruit drop. Sodium p-chlorophenoxyacetate (also known as anti-drop agent) is a plant growth regulator. At present, it is only registered on tomatoes in my country, and farmers often use it for spraying before the bayberry matures and is picked. However, as a chemical reagent, sodium p-chlorophenoxyacetate residues still pose a risk of chronic dietary exposure because bayberry fruits have no skin protection. However, there are currently few registered pesticide varieties for bayberry, and the safety requirements for the use of drugs as ready-to-eat fruits are high, so natural, green, safe, and effective anti-fruit drop compounds are urgently needed.

[0004] Baipichattanol is a polyhydroxyphenol compound (stilbene-type compound) with diphenylethylene as the basic nucleus, in which hydrogen atoms at different positions of the benzene ring are replaced by hydroxyl groups. It is a natural plant metabolite with the characteristics of being green, safe and non-toxic. It is widely found in fruit and vegetable plants such as grapes, blueberries, and passion fruit, and participates in multiple metabolic pathways of plants. At present, Baipichattanol is mainly used in the medical field. A large number of experiments have confirmed that this type of compound is a protein tyrosine kinase (Syk) inhibitor with significant biological activities such as antioxidant, free radical scavenging, cardiovascular and cerebrovascular protection, and anti-malignant tumor. It has a strong antibacterial effect. However, there have been no reports on its application in preventing fruit drop in plants. Summary of the invention

[0005] The purpose of the present invention is to provide an application of picrosanol in preventing bayberry fruit drop, so as to solve the problem of harmful residues in pesticides for preventing bayberry fruit drop.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The invention provides application of picrosanol in preventing bayberry fruit drop.

[0008] Preferably, the bayberry trees are sprayed with an aqueous solution of baicalin.

[0009] Preferably, the concentration of picranthol in the picranthol aqueous solution is 0.8-1.2 mg / L.

[0010] Preferably, the spraying period is the hard core stage, whitening stage and / or redding stage of bayberry fruit development.

[0011] Preferably, the spraying site is the leaf surface and / or petiole of the bayberry tree.

[0012] The invention also provides a medicinal agent for preventing bayberry fruit drop, comprising picrosanthemol.

[0013] By adopting the above technical scheme, the present invention has the following beneficial effects: the present invention provides the application of picotol in preventing bayberry from dropping fruit. Picotol is a natural plant metabolite. After spraying bayberry plants with a picotol aqueous solution, the fruit drop prevention effect is significant. The test results show that after spraying the picotol aqueous solution, the bayberry fruit drop rate is reduced from 43.99% to 17.73%, and there is no pesticide residue in the bayberry when it is harvested, which is green, safe and non-toxic. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is the fruit drop rate of bayberry after the bayberry plants were treated with picrocarpon and anti-drop agent.

[0015] Figure 2 The content of picotol in bayberry fruits after the bayberry plants were treated with picotol and anti-drop agent.

[0016] Figure 3 The content of auxin in bayberry fruit after the bayberry plants were treated with picranthorhin and anti-drop agent.

[0017] Figure 4 The content of zeatin in bayberry fruit after the bayberry plants were treated with zeatin and zeatin.

[0018] Figure 5 The content of abscisic acid in bayberry fruit after the bayberry plants were treated with picrocarpon and anti-falling agent. DETAILED DESCRIPTION

[0019] The present invention provides the application of picotol in preventing bayberry fruit drop. The present invention achieves the purpose of preventing bayberry fruit drop by spraying a picotol aqueous solution on bayberry fruit trees. The concentration of picotol in the picotol aqueous solution of the present invention is 0.8-1.2 mg / L, more preferably 0.9-1.1 mg / L, more preferably 1 mg / L, and the spraying period is the hard core stage, whitening stage and / or redning stage of bayberry fruit development; the spraying site is the leaf surface and / or petiole of the bayberry fruit tree, and the spraying is performed until the front and back sides of the leaf surface are completely wet without dripping water.

[0020] The invention also provides a medicinal agent for preventing bayberry fruit drop, which contains picrosanthemol.

[0021] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0022] Example 1

[0023] In this embodiment, a 1 mg / L picrosanol solution is sprayed on the leaves and petioles of bayberry plants during the hard core stage, white stage and red stage of bayberry fruits until both sides of the leaves are completely wet without dripping, and the spraying is performed once every 14 days.

[0024] Example 2

[0025] In this embodiment, a 0.8 mg / L picrosanol solution is sprayed on the leaves and petioles of bayberry plants during the hard core stage, white stage and red stage of bayberry fruit until both sides of the leaves are completely wet without dripping, and the spraying is performed once every 14 days.

[0026] Example 3

[0027] In this embodiment, a 1.2 mg / L picrosanol solution was sprayed on the leaves and petioles of bayberry plants during the hard core stage, white stage and red stage of bayberry fruit until both sides of the leaves were completely wet without dripping, and the spraying was performed once every 14 days.

[0028] Comparative Example 1

[0029] The difference from Example 1 is that 40 mg / L of anti-drop agent was sprayed during the hard core stage, white stage and red stage of the bayberry fruit.

[0030] Experimental Example 1

[0031] The experiment was conducted on April 22, 2024 in Chashan, Ouhai District, Wenzhou City. The bayberry variety was "Dingao". The experiment was divided into three groups, with 3 bayberries in each group. The bayberries were sprayed according to the methods of Example 1 and the comparative example, and the control group was sprayed with an equal amount of deionized water. Spraying was performed once every 14 days, for a total of 3 times. The fruit drop rate was calculated after the fruit was fully mature, that is, during the ripening period. The results are as follows Figure 1 shown.

[0032] Fruit drop rate (%) = (number of fruits before application - number of fruits after application) / number of fruits before application × 100.

[0033] The results showed that the fruit drop rate of the control group was more serious, reaching 43.99%, and the fruit drop rate of the bayberry sprayed with baicalin was 17.73%, which was about 26.26% lower than that of the control group, and the fruit drop rate of the sprayed anti-fruit drop agent was 23.77%, which was about 20.22% lower than that of the control group. This shows that baicalin has a significant effect on preventing bayberry from falling, and its anti-fruit drop effect is better than the commonly used anti-fruit drop agents on the market.

[0034] In this experiment, after spraying picrosan and anti-drop agent respectively at the red stage of bayberry, any treatment with other drugs was stopped. At the ripening stage of bayberry fruit, the changes of picrosan content in the control, picrosan and anti-drop agent treated fruits were measured respectively. The results are as follows: Figure 2 The results showed that as a natural compound, picathanol is present in a small amount in mature bayberry fruits, indicating that picathanol is a natural compound. After the bayberry was treated with anti-drop agent, not only the fruit drop rate was high, but the content of picathanol in the fruit was also low, further indicating that picathanol can prevent and control bayberry fruit drop.

[0035] Auxin (IAA) has the function of promoting fruit development and inhibiting abscission layer formation, while zeatin (ZT) is closely related to plant growth and development and has the function of promoting fruit setting. After the fruit is fully mature, that is, in the ripening period, the content of auxin and zeatin in bayberry fruit is determined. The results are as follows Figure 3 , Figure 4The results showed that after being treated with picotol, the content of auxin and zeatin in bayberry fruit increased significantly, and was higher than that of bayberry sprayed with anti-drop agent, indicating that spraying picotol during fruit development is conducive to a substantial increase in the content of auxin and zeatin in mature bayberry fruit. The significant increase in the content of auxin and zeatin can inhibit the formation of abscission layer and promote the fruit setting of bayberry, thus achieving a significant effect of preventing bayberry from fruit drop.

[0036] Abscisic acid (ABA) can induce plant leaf and fruit drop by regulating the activity of cell separation enzymes in the fruit shedding area, causing the fruit cell wall to be degraded and separated from the fruit branch or fruit axis, thereby achieving the effect of promoting fruit shedding. While determining the auxin and zeatin in bayberry fruit, the abscisic acid content in bayberry fruit was also determined. The results are as follows Figure 5 shown. Figure 5 The results showed that when bayberry plants were sprayed with baicalin, the abscisic acid content in the mature fruits of bayberry decreased, indicating that baicalin has a significant positive effect in preventing bayberry fruit drop.

[0037] As can be seen from the above examples, the present invention provides the use of picotol in preventing bayberry fruit drop. The present invention can effectively prevent bayberry fruit drop by spraying the picotol aqueous solution on bayberry plants.

[0038] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. Application of baicalin in preventing bayberry fruit drop.

2. The use according to claim 1, characterized in that: Spray the bayberry trees with an aqueous solution of white bark alcohol.

3. The use according to claim 2, characterized in that: The concentration of picrosanol in the picrosanol aqueous solution is 0.8-1.2 mg / L.

4. The use according to claim 3, characterized in that: The spraying period is the hard core stage, whitening stage and / or redning stage of the bayberry fruit development.

5. The use according to claim 4, characterized in that: The spraying site is the leaf surface and / or petiole of the bayberry tree.

6. A medicament for preventing bayberry fruit drop, characterized in that: Contains picrosanthellol.

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