Soybean root rot seed coating agent

Abstract

The present invention relates to a soybean root rot seed coating agent, and belongs to the technical field of crop disease control, and the soybean root rot seed coating agent comprises rare earth, boric acid, scarlet, sodium carboxymethyl starch, ammonium molybdate, zinc sulfate, vitamin protein and carbendazim. Compared with the prior art, the seed coating agent has the advantages that the effect is good, the prevention and treatment effect of the formula agent on the soybean root rot is obviously improved compared with that of a single fungicide, the seed coating agent has the characteristic of efficient prevention and treatment on diseases, and pesticide effect tests show that the prevention effect of the seed coating agent product on the soybean root rot can reach 82%; the seed coating agent prepared by the method disclosed by the invention and the seed coating agent for preventing and treating the soybean root rot are free from special instruments and technologies, simple and convenient to operate, safe and reliable, simple and feasible in preparation method, low in cost and suitable for large-scale popularization, and have important practical value, economic benefit and ecological protection benefit.

Description

Technical Field

[0001] The invention relates to the technical field of crop disease prevention and control, and in particular to a soybean root rot seed coating agent. Background Art

[0002] Soybean is an important food crop and oil crop in my country, and an important source of protein for human food. Although my country ranks fifth in the world in terms of soybean production and harvested area, the soybean yield per unit area is still far behind the average yield of 3,500 kg / hm2 in the United States. In addition to factors such as varieties, cultivation and production levels, the losses caused by pests and diseases account for about 20%. Soybean root rot caused by pathogens such as Fusarium, Pythium and Rhizoctonia solani occurs in the Northeast Black Soil Area and Huanghuaihai Region, the main soybean producing areas in my country. It is an important soil-borne disease in soybean production, generally reducing production by 10% to 60%. The damage caused by soybean planting in succession is more serious, becoming one of the dominant factors limiting soybean production. Although the selection and promotion of disease-resistant varieties and agricultural cultivation measures play an active role in the prevention and control of crop diseases, chemical pesticides are still an important means of crop disease prevention and control. The commonly used measure for the prevention and control of soybean root rot is chemical seed coating treatment. Since chemical pesticides cannot be completely replaced, researching efficient and safe soybean disease prevention and control technologies, reducing pesticide inputs, and achieving sustainable management of crop diseases have enormous social, economic, and scientific and technological development significance.

[0003] Seed coating agents are based on the types and harmful characteristics of soil-borne diseases of crops. They coat the surface of seeds with a layer of fungicide-containing coating to prevent and control diseases at the seedling stage, so as to achieve the goal of crop yield. The working principle is that the seed coating agent forms a water-insoluble but breathable and water-permeable coating film with membrane pores on the surface of the seeds, which binds the active ingredients such as fungicides with the inactive ingredients. When the seeds are sown in the soil, the seed coating only absorbs water but does not dissolve. Its active ingredients are released very slowly through the membrane pores and gradually contact the surface of the seeds and the adjacent soil, thereby killing the seed-borne pathogens and forming a protective barrier around the seeds, playing the role of disinfecting the seeds and preventing the infection of soil-borne pathogens. As the seeds germinate and emerge from the soil, the agent is slowly released in the underground roots, and is conducted to the un-medicated above-ground parts through plant absorption, continuing to play the role of preventing and controlling diseases. Seed dressing agents overcome the drawbacks of conventional seed treatments. They have concentrated drug efficacy, high utilization rate, and direct contact with the soil. They are not easily affected by environmental conditions such as sunlight, rain, and high temperature. The drug efficacy period can be as long as 45-60 days, achieving the effects of preventing and controlling soil-borne diseases, promoting seedling growth, and increasing crop yield and quality. At present, seed dressing agents have been widely used in my country and have achieved obvious results. However, there are very few seed dressing agents used to prevent and control soybean root rot. Among the few seed dressing agents produced to prevent and control soybean root rot, the active ingredients that have a bactericidal effect are mostly single fungicides, and the control effect is not ideal. Three fungicides, such as oxadiazine, thiram, and oxadiazine, have good control effects on diseases caused by oomycetes and soil-borne pathogenic fungi, and are particularly suitable for soil and seed treatment. Summary of the invention

[0004] The present invention aims to solve the above technical problems and provides a soybean root rot seed coating agent.

[0005] In order to solve the above technical problems, the technical solution provided by the present invention is:

[0006] A soybean root rot seed coating agent comprises 0.02-0.07g of rare earth, 0.02-0.04g of boric acid, 0.01-0.05g of scarlet, 0.1-0.3g of sodium carboxymethyl starch, 0.1-0.3g of ammonium molybdate, 0.1-0.3g of zinc sulfate, 0.10.1-0.3g of vitamin C and 1.0-1.5g of carbendazim.

[0007] Preferably, it includes 0.05g rare earth, 0.033g boric acid, 0.03g scarlet, 0.12g sodium carboxymethyl starch, 0.13g ammonium molybdate, 0.17g zinc sulfate, 0.25g vitrin and 1.39g carbendazim.

[0008] Preferably, the effective content of the rare earth is 96%.

[0009] Preferably, the effective content of the boric acid and zinc sulfate is 99.5%.

[0010] Preferably, the effective content of the ammonium molybdate and sodium carboxymethyl starch is 99%.

[0011] Preferably, the active ingredient of the vitamin D protein is 2%.

[0012] Preferably, the active ingredient of carbendazim is 50%.

[0013] A method for testing a soybean root rot seed coating agent comprises the following steps:

[0014] S1. Preparation of soybean root rot pathogen inoculum:

[0015] The Fusarium oxysporum M33 strain stored on the slant was activated and transferred to PDA medium, cultured for 6-8 days, and set aside. The sorghum grains were boiled for 25-35 minutes, 180-210 g were taken and placed in canning bottles, and sterilized by high pressure for 110-130 minutes. Five activated pathogenic bacteria discs with a diameter of 6-8 mm were added to each bottle, and cultured at 20-30°C for 8-12 days to obtain sorghum grain inoculants, which were set aside.

[0016] S2. Chemical treatment:

[0017] After accurately weighing the components of the formula of the soybean root rot seed coating agent, put it in a container, add 3-4 mL of warm water, stir evenly, weigh 240-260 g of Heinong 82 soybean seeds, disinfect the surface, select uniform seeds, stir evenly, let it stand for 15-25 minutes, then take it out and dry it for later use;

[0018] S3. Potted plant treatment:

[0019] Each plastic nutrient pot was filled with sterilized soil to 2 / 3, and 6-10g of cultured sorghum grains were placed in it, and then covered with 2cm of sterilized soil, and 8 coated soybean seeds were sown, and then covered with sterilized soil. Three plastic nutrient pots were used for each treatment. The treated plastic nutrient pots were placed in a greenhouse. The culture conditions were 20-30℃, light and dark alternation for 10-14 hours. During the growth period of the plants, water was applied quantitatively for each treatment. The disease status of the plants was investigated after 28-32 days of culture.

[0020] S4. Disease evaluation:

[0021] Disease index = 100 × ∑ (number of diseased plants at each level × relative level value) / (total number of plants investigated × highest level);

[0022] Control effect = [(control disease index - control disease index) / control disease index] × 100%.

[0023] After adopting the above formula, the present invention has the following advantages:

[0024] 1. Good effect: The bactericidal effect of the formula agent of the present invention on soybean root rot is significantly increased compared with that of a single bacterial agent. The seed coating agent of the present invention has the characteristics of high efficiency in preventing and controlling diseases. The efficacy experiment shows that the seed coating agent product of the present invention can achieve a prevention effect of 82% on soybean root rot;

[0025] 2. Simple and fast operation: The method of the present invention is used to prepare the seed coating agent and use the seed coating agent to prevent and treat soybean root rot, without the need for special instruments and techniques, and the operation is simple, convenient, safe and reliable.

[0026] 3. The preparation method is simple and easy, low-cost, suitable for large-scale promotion, and has important practical value, economic benefits and environmental protection benefits.

[0027] The above summary is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments and features described above, further aspects, embodiments and features of the present invention will be readily apparent by reference to the accompanying drawings and the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or technical descriptions will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0029] Figure 1 This is a mycelium diagram of the soybean root rot pathogen of the present invention;

[0030] Figure 2 It is a diagram of a soybean plant suffering from disease of the present invention;

[0031] Figure 3 This is a soybean diagram of the present invention after the first and second embodiments are implemented. DETAILED DESCRIPTION

[0032] Specific embodiments of the present invention will now be mentioned in detail. Although the present invention is described in conjunction with these specific embodiments, it should be appreciated that it is not intended to limit the present invention to these specific embodiments. On the contrary, these embodiments are intended to cover substitutions, changes or equivalent embodiments that may be included in the spirit and scope of the invention defined by the claims. In the following description, a large number of specific details are set forth in order to provide a comprehensive understanding of the present invention. The present invention can be implemented without some or all of these specific details. In other cases, in order not to make the present invention unnecessarily obscure, well-known process operations are not described in detail.

[0033] When used in conjunction with "including," "methods comprising," or similar language in this specification and the appended claims, the singular forms "a," "an," and "the" include plural references unless the context clearly dictates otherwise. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0034] The present invention is further described in detail below in conjunction with the full text.

[0035] Combined with Figure 1-Figure 3 A soybean root rot seed coating agent, including rare earth 0.02-0.07g, boric acid 0.02-0.04g, red 0.01-0.05g, sodium carboxymethyl starch 0.1-0.3g, ammonium molybdate 0.1-0.3g, zinc sulfate 0.1-0.3g, vitamin D protein 0.10.1-0.3g and carbendazim 1.0-1.5g.

[0036] It includes 0.05g rare earth, 0.033g boric acid, 0.03g scarlet, 0.12g sodium carboxymethyl starch, 0.13g ammonium molybdate, 0.17g zinc sulfate, 0.25g vitamin C and 1.39g carbendazim.

[0037] The effective content of the rare earth is 96%.

[0038] The effective content of the boric acid and zinc sulfate is 99.5%.

[0039] The effective components of the ammonium molybdate and sodium carboxymethyl starch are 99%.

[0040] The effective component of the vitamin D protein is 2%.

[0041] The effective component of the carbendazim is 50%.

[0042] (1) Rare earth: Active ingredient 96%, selected from Chengdu Huahong Biotechnology Co., Ltd. or rare earth with equivalent effect.

[0043] (2) Boric acid: active ingredient 99.5%, selected from Tianjin Tianda Chemical Reagent Factory or equivalent.

[0044] (3) Sodium carboxymethyl starch: 99% active ingredient, from Shanghai McLean Biochemical Technology Co., Ltd. or equivalent.

[0045] (4) Ammonium molybdate: Active ingredient 99%, select ammonium molybdate from Tianjin Hengxin Chemical Co., Ltd. or equivalent.

[0046] (5) Zinc sulfate: The active ingredient is 99.5%. Zinc sulfate from Tianjin Ruijinte Chemical Co., Ltd. or equivalent should be used.

[0047] (6) Vitamin D protein: 2% active ingredient

[0048] (7) Carbendazim: 50% active ingredient, selected from Jiangsu Lanfeng Biochemical Co., Ltd. or carbendazim with equivalent effect.

[0049] A method for testing a soybean root rot seed coating agent comprises the following steps:

[0050] S1. Preparation of soybean root rot pathogen inoculum:

[0051] The Fusarium oxysporum M33 strain stored on the slant was activated and transferred to PDA medium, cultured for 6-8 days, and set aside. The sorghum grains were boiled for 25-35 minutes, 180-210 g were taken and placed in canning bottles, and sterilized by high pressure for 110-130 minutes. Five activated pathogenic bacteria discs with a diameter of 6-8 mm were added to each bottle, and cultured at 20-30°C for 8-12 days to obtain sorghum grain inoculants, which were set aside.

[0052] S2. Chemical treatment:

[0053] After accurately weighing the components of the formula of the soybean root rot seed coating agent, put it in a container, add 3-4 mL of warm water, stir evenly, weigh 240-260 g of Heinong 82 soybean seeds, disinfect the surface, select uniform seeds, stir evenly, let it stand for 15-25 minutes, then take it out and dry it for later use;

[0054] S3. Potted plant treatment:

[0055] Each plastic nutrient pot was filled with sterilized soil to 2 / 3, and 6-10g of cultured sorghum grains were placed in it, and then covered with 2cm of sterilized soil, and 8 coated soybean seeds were sown, and then covered with sterilized soil. Three plastic nutrient pots were used for each treatment. The treated plastic nutrient pots were placed in a greenhouse. The culture conditions were 20-30℃, light and dark alternation for 10-14 hours. During the growth period of the plants, water was applied quantitatively for each treatment. The disease status of the plants was investigated after 28-32 days of culture.

[0056] S4. Disease evaluation:

[0057] Disease index = 100 × ∑ (number of diseased plants at each level × relative level value) / (total number of plants investigated × highest level);

[0058] Control effect = [(control disease index - control disease index) / control disease index] × 100%.

[0059] Soil matrix for the test: The soil was collected from the cultivated layer of soybean fields in the Minzhu Park of Heilongjiang Academy of Agricultural Sciences.

[0060] Test pathogen: Fusarium oxysporum strain M33.

[0061] Soybean variety tested: Heinong 82.

[0062] Embodiment 1:

[0063] A soybean root rot seed coating agent comprises 0.05 g of rare earth, 0.032 g of boric acid, 0.02 g of scarlet, 0.2 g of sodium carboxymethyl starch, 0.2 g of ammonium molybdate, 0.2 g of zinc sulfate, 0.15 g of vitamin C and 1.2 g of carbendazim.

[0064] A method for testing a soybean root rot seed coating agent comprises the following steps:

[0065] S1. Preparation of soybean root rot pathogen inoculum:

[0066] The Fusarium oxysporum M33 strain stored on the slant was activated and transferred to PDA medium, cultured for 7 days, and set aside. The sorghum grains were boiled for 30 minutes, 190 g was taken and placed in a canning bottle, and sterilized by high pressure for 120 minutes. Five activated pathogenic bacteria discs with a diameter of 7 mm were added to each bottle, and cultured at 25°C for 9 days to obtain sorghum grain inoculants, which were set aside.

[0067] S2. Chemical treatment:

[0068] After accurately weighing the components of the formula of the soybean root rot seed coating agent, put it in a container, add 4 mL of warm water, stir evenly, weigh 250 g of Heinong 82 soybean seeds, disinfect the surface, select uniform seeds, stir evenly, let it stand for 18 minutes, then take it out and dry it for later use;

[0069] S3. Potted plant treatment:

[0070] Each plastic nutrient pot was filled with sterile soil to 2 / 3, and 6g of cultured sorghum grains were placed in it, and then covered with 2cm of sterile soil, and 8 coated soybean seeds were sown, and then covered with sterile soil. Three plastic nutrient pots were used for each treatment. The treated plastic nutrient pots were placed in a greenhouse. The culture conditions were a temperature of 22℃, light and dark alternation for 13 hours. During the growth period of the plants, water was irrigated quantitatively for each treatment. The disease status of the plants was investigated after 29 days of cultivation.

[0071] S4. Disease evaluation:

[0072] Disease index = 100 × ∑ (number of diseased plants at each level × relative level value) / (total number of plants investigated × highest level);

[0073] Control effect = [(control disease index - control disease index) / control disease index] × 100%.

[0074] Embodiment 2:

[0075] A soybean root rot seed coating agent comprises 0.05 g of rare earth, 0.033 g of boric acid, 0.03 g of scarlet, 0.12 g of CMC (sodium carboxymethyl starch), 0.13 g of ammonium molybdate, 0.17 g of zinc sulfate, 0.25 g of vitamin C and 1.39 g of carbendazim.

[0076] A method for testing a soybean root rot seed coating agent comprises the following steps:

[0077] S1. Preparation of soybean root rot pathogen inoculum:

[0078] The Fusarium oxysporum M33 strain stored on the slant was activated and transferred to PDA medium, cultured for 7 days, and set aside. Boil the sorghum grains for 30 minutes, take 200g and put them into canning bottles, and sterilize them by high pressure for 120 minutes. Each bottle was inoculated with 5 activated pathogenic bacteria discs with a diameter of 7mm, cultured at 25℃ for 10 days, and set aside.

[0079] S2. Chemical treatment:

[0080] After accurately weighing the components of the formula, put them in a container, add 3.33 mL of warm water and stir evenly, weigh 250 g of Heinong 82 soybean seeds, disinfect the surface (rinse with tap water for 15 minutes, disinfect with 70% alcohol for 50 seconds, and disinfect with 6% sodium hypochlorite disinfectant for 15 minutes), select uniform seeds, stir evenly and let them stand for 20 minutes, then take them out and dry them for later use;

[0081] S3. Potted plant treatment:

[0082] Each plastic nutrient pot (14 cm in diameter) was filled with sterilized soil to 2 / 3, and 8 g of cultured sorghum grains were placed, and then covered with 2 cm of sterilized soil, 8 coated soybean seeds were sown, and then covered with sterilized soil. Three plastic nutrient pots were used for each treatment (repeated 3 times). The treated plastic nutrient pots were placed in a greenhouse, and the culture conditions were a temperature of 25°C and alternating light and dark for 12 hours. During the growth period of the plants, water was irrigated quantitatively for each treatment. The disease status of the plants was investigated after 30 days of cultivation.

[0083] S4. Disease evaluation:

[0084] Disease index = 100 × ∑ (number of diseased plants at each level × relative level value) / (total number of plants investigated × highest level);

[0085] Control effect = [(control disease index - control disease index) / control disease index] × 100%.

[0086] Test example 1:

[0087] Test example 1 is a blank CK without treatment.

[0088] The following steps are involved:

[0089] Each plastic nutrient pot (14 cm in diameter) was filled with sterilized soil to 2 / 3, and 8 g of good sorghum grains were placed, and then covered with 2 cm of sterilized soil, and 8 coated soybean seeds were sown, and then covered with sterilized soil. Three plastic nutrient pots were used for each treatment (repeated 3 times). The treated plastic nutrient pots were placed in a greenhouse, and the culture conditions were a temperature of 25°C and alternating light and dark for 12 hours. During the growth period of the plants, water was irrigated quantitatively for each treatment. The disease status of the plants was investigated after 30 days of cultivation.

[0090] Test Example 2:

[0091] Test example 2 is the inoculation control.

[0092] The following steps are involved:

[0093] S1. Preparation of soybean root rot pathogen inoculum:

[0094] The Fusarium oxysporum M33 strain stored on the slant was activated and transferred to PDA medium, cultured for 7 days, and set aside. Boil the sorghum grains for 30 minutes, take 200g and put them into canning bottles, and sterilize them by high pressure for 120 minutes. Each bottle was inoculated with 5 activated pathogenic bacteria discs with a diameter of 7mm, cultured at 25℃ for 10 days, and set aside.

[0095] S2. Potted plant treatment:

[0096] Each plastic nutrient pot (14 cm in diameter) was filled with sterilized soil to 2 / 3, and 8 g of cultured sorghum grains were placed, and then covered with 2 cm of sterilized soil, 8 coated soybean seeds were sown, and then covered with sterilized soil. Three plastic nutrient pots were used for each treatment (repeated 3 times). The treated plastic nutrient pots were placed in a greenhouse, and the culture conditions were a temperature of 25°C and alternating light and dark for 12 hours. During the growth period of the plants, water was irrigated quantitatively for each treatment. The disease status of the plants was investigated after 30 days of cultivation.

[0097] Test Example 3:

[0098] Test example 3 is a control of soybean treated with Liangdun seed coating agent.

[0099] The following steps are involved:

[0100] S1. Preparation of soybean root rot pathogen inoculum:

[0101] The Fusarium oxysporum M33 strain stored on the slant was activated and transferred to PDA medium, cultured for 7 days, and set aside. Boil the sorghum grains for 30 minutes, take 200g and put them into canning bottles, and sterilize them by high pressure for 120 minutes. Each bottle was inoculated with 5 activated pathogenic bacteria discs with a diameter of 7mm, cultured at 25℃ for 10 days, and set aside.

[0102] S2. Potted plant treatment:

[0103] Each plastic nutrient pot (14 cm in diameter) was filled with sterilized soil to 2 / 3, and 8 g of cultured sorghum grains were placed, and then covered with 2 cm of sterilized soil, 8 coated soybean seeds were sown, and then covered with sterilized soil. Three plastic nutrient pots were used for each treatment (repeated 3 times). The treated plastic nutrient pots were placed in a greenhouse, and the culture conditions were a temperature of 25°C and alternating light and dark for 12 hours. During the growth period of the plants, water was irrigated quantitatively for each treatment. The disease status of the plants was investigated after 30 days of cultivation.

[0104] The coating agent treatment tables of Example 1, Example 2, Test Example 1, Test Example 2 and Test Example 3 are as follows:

[0105] Table 1 Seed dressing treatment

[0106] Test No. Soil treatment Test Example 1 Blank CK Test Example 2 Inoculation control Test Example 3 Liangdun seed coating agent Embodiment 1 Seed coating formulation Embodiment 2 Seed coating formulation

[0107] Statistical analysis:

[0108] SPSS data processing system was used for analysis of variance and Duncan's multiple comparisons.

[0109] Results and analysis:

[0110] The pathogen Fusarium oxysporum was isolated from the roots of diseased soybean plants by tissue separation method. After plate culture, the mycelium was picked out and observed under a microscope. Figure 1 As shown, Figure 2 It is a diseased soybean plant. Figure 3 It is a soybean plant after passing through Example 1 and Example 2.

[0111] The survey results show (as shown in Table 2) that the control agent Liangdun has a control effect of 81.25%, and the control effects of the homemade seed dressing agent are 82.50% and 82.40%, respectively. Their control effects are slightly higher than those of the control agent, showing better control effects.

[0112] Table 2 The control effect of seed coating formulation on soybean root rot

[0113]

[0114]

[0115] The present invention and its embodiments are described above, and such description is not restrictive. What is shown in the full text is only one of the embodiments of the present invention, and the actual structure is not limited thereto. In short, if ordinary technicians in the field are inspired by it, without departing from the purpose of the invention, they can design a structure and embodiment similar to the technical solution without creativity, which should belong to the protection scope of the present invention.

Claims

1. A soybean root rot seed coating agent, characterized in that: It includes rare earth 0.02-0.07g, boric acid 0.02-0.04g, scarlet 0.01-0.05g, sodium carboxymethyl starch 0.1-0.3g, ammonium molybdate 0.1-0.3g, zinc sulfate 0.1-0.3g, vitamin C 0.10.1-0.3g and carbendazim 1.0-1.5g.

2. A soybean root rot seed coating agent according to claim 1, characterized in that: It includes 0.05g rare earth, 0.033g boric acid, 0.03g scarlet, 0.12g sodium carboxymethyl starch, 0.13g ammonium molybdate, 0.17g zinc sulfate, 0.25g vitamin C and 1.39g carbendazim.

3. A soybean root rot seed coating agent according to claim 1 or 2, characterized in that: The effective content of the rare earth is 96%.

4. A soybean root rot seed coating agent according to claim 1 or 2, characterized in that: The effective content of the boric acid and zinc sulfate is 99.5%.

5. A soybean root rot seed coating agent according to claim 1 or 2, characterized in that: The effective components of the ammonium molybdate and sodium carboxymethyl starch are 99%.

6. A soybean root rot seed coating agent according to claim 1 or 2, characterized in that: The effective component of the vitamin D protein is 2%.

7. A soybean root rot seed coating agent according to claim 1 or 2, characterized in that: The effective component of the carbendazim is 50%.

8. A method for testing a soybean root rot seed coating agent, characterized in that: The following steps are involved: S1. Preparation of soybean root rot pathogen inoculum: The Fusarium oxysporum M33 strain stored on the slant was activated and transferred to PDA medium, cultured for 6-8 days, and set aside. The sorghum grains were boiled for 25-35 minutes, 180-210 g were taken and put into canning bottles, and sterilized by high pressure for 110-130 minutes. Five activated pathogenic bacteria discs with a diameter of 6-8 mm were added to each bottle, and cultured at 20-30° C. for 8-12 days to obtain sorghum grain inoculants, which were set aside. S2. Chemical treatment: After accurately weighing the components of the formula of the soybean root rot seed coating agent, put it in a container, add 3-4 mL of warm water, stir evenly, weigh 240-260 g of Heinong 82 soybean seeds, disinfect the surface, select uniform seeds, stir evenly, let it stand for 15-25 minutes, then take it out and dry it for later use; S3. Potted plant treatment: Each plastic nutrient pot was filled with sterilized soil to 2 / 3, and 6-10g of cultured sorghum grains were placed in it, and then covered with 2cm of sterilized soil, and 8 coated soybean seeds were sown, and then covered with sterilized soil. Three plastic nutrient pots were used for each treatment. The treated plastic nutrient pots were placed in a greenhouse. The culture conditions were 20-30℃, light and dark alternation for 10-14 hours. During the growth period of the plants, water was applied quantitatively for each treatment. The disease status of the plants was investigated after 28-32 days of culture. S4. Disease evaluation: Disease index = 100 × ∑ (number of diseased plants at each level × relative level value) / (total number of plants investigated × highest level); Control effect = [(control disease index - control disease index) / control disease index] × 100%.

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