Seaweed composite microspheres capable of relaxing bowels as well as preparation method and application of seaweed composite microspheres

Abstract

The invention discloses a seaweed composite microsphere capable of relaxing bowels as well as a preparation method and application thereof, and belongs to the technical field of food processing. The seaweed composite microsphere is prepared from the following components in parts by weight: 5 to 10 parts of sodium alginate, 1 to 5 parts of fucoidin and 1 to 2 parts of rutabaga root extract. The seaweed composite microspheres can relax bowel and quickly change constipation symptoms, are safe and free of toxic and side effects, and can be used for a long time. Through verification, the traditional Chinese medicine composition can take effect within 3-4 h after being eaten, the defecation amount is increased, the water content of excrement is increased, the intestinal tract propelling rate is increased, intestinal tract movement is promoted, the constipation condition can be prevented and relieved, and no obvious adverse reaction exists. When the seaweed composite microspheres are taken after being mixed with water, the seaweed composite microspheres can be rapidly dispersed in water to form uniform suspension liquid, and the suspension liquid has viscosity similar to that of porridge, but has no viscosity of polysaccharide and is smooth in taste; the food can achieve ideal taste without long-time stirring, is suitable for long-term taking, improves the acceptability of the public, and is more suitable for a fast-paced lifestyle.

Description

Technical Field

[0001] The invention belongs to the technical field of food processing, and in particular relates to a seaweed composite microsphere for moistening the intestine and facilitating bowel movements, and a preparation method and application thereof. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention, and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.

[0003] Constipation is one of the common intestinal diseases in clinical practice, which is usually manifested by decreased bowel movement frequency, difficulty in defecation or dry and hard stools.

[0004] There are many causes of constipation.

[0005] 1. Lifestyle factors:

[0006] Lack of sufficient fiber in the diet or insufficient water intake can lead to dry and hard stools; a high-fat diet causes oil to cover the intestinal walls and slow intestinal movement; lack of exercise or ignoring the body's bowel movement signals can cause feces to stay in the intestines too long, causing excessive absorption of water, and the stool becomes dry and hard, leading to constipation.

[0007] 2. Physiological factors:

[0008] As we age, the motility of intestinal muscles decreases, leading to the degeneration of intestinal function, which slows down the movement of food residues in the intestines and causes constipation.

[0009] 3. Medical factors

[0010] The use of painkillers, antidepressants, calcium channel blockers and other drugs may slow down intestinal motility and cause constipation; chemotherapy drugs, such as vincristine, cyclophosphamide, etc., can damage the intestinal nervous system and autonomic nervous system, affect the intestinal mucosa, and cause diarrhea or constipation.

[0011] 4. Mental and psychological factors caused by high work pressure

[0012] Being under great work pressure for a long time, psychological and mental factors such as tension and anxiety can easily lead to constipation.

[0013] The abdominal bloating and abdominal pain caused by constipation will affect the patient's daily comfort. Long-term constipation may cause anxiety, depression or mood swings, causing physical and mental distress to the patient. With the acceleration of the pace of modern life and the increase of work pressure, constipation has become increasingly common.

[0014] At present, the main methods to solve the problem of constipation include adjusting eating habits, increasing exercise, reducing mental stress, and drug treatment. Among them, drug treatment is one of the common methods for treating constipation. Common constipation treatment drugs mainly include bulk laxatives, osmotic laxatives, stimulant laxatives, lubricating laxatives, prokinetic drugs, secretagogues, and suppository laxatives, etc. However, common constipation treatment drugs are highly irritating and difficult to use for special groups such as the elderly, pregnant women, and children. Long-term use may cause side effects or dependence, and cannot fundamentally solve the problem.

[0015] In the development of oral preparations, taste is an important consideration, especially in the field of functional foods and health products. Good taste can improve patient compliance, especially for products that need to be taken for a long time. Natural ingredients, such as sodium alginate and fucoidan, have a laxative effect when consumed for a long time, but traditional sodium alginate and fucoidan preparations often have an overly viscous texture, which affects consumer acceptance. Therefore, it is of great significance to develop products that can moisturize the intestines, quickly improve constipation symptoms, are safe, have no toxic side effects, and have high acceptance. Summary of the invention

[0016] In order to solve the deficiencies of the prior art, the purpose of the present invention is to provide a laxative seaweed composite microsphere and a preparation method and application thereof. The laxative seaweed composite microsphere provided by the present invention can moisturize the intestine and promote bowel movements, quickly change constipation symptoms, is safe and has no toxic side effects, and can be used for a long time.

[0017] In order to achieve the above object, the technical solution of the present invention is:

[0018] The first aspect of the present invention provides a laxative seaweed composite microsphere, which comprises, by weight, 5-10 parts of sodium alginate, 1-5 parts of fucoidan, and 1-2 parts of a root extract of Psoralea corylifolia.

[0019] In some embodiments of the present invention, the laxative seaweed composite microspheres include 5-6 parts of sodium alginate, 3-4 parts of fucoidan, and 1-2 parts of Psoralea corylifolia root extract.

[0020] In some embodiments of the present invention, the laxative seaweed composite microspheres include 6 parts of sodium alginate, 3 parts of fucoidan, and 2 parts of Psoralea corylifolia root extract.

[0021] In some embodiments of the present invention, the laxative seaweed composite microspheres include 5 parts of sodium alginate, 3 parts of fucoidan, and 2 parts of Psoralea corylifolia root extract.

[0022] In some embodiments of the present invention, the laxative seaweed composite microspheres include 6 parts of sodium alginate, 2 parts of fucoidan, and 2 parts of Psoralea corylifolia root extract.

[0023] In some embodiments of the present invention, the Herba Lycopodii root extract is obtained by decocting and freeze-drying the Herba Lycopodii roots.

[0024] In some embodiments of the present invention, the particle size of the intestinal moistening and laxative seaweed composite microspheres is 90-200 μm.

[0025] The second aspect of the present invention provides a method for preparing the above-mentioned intestinal moistening and laxative seaweed composite microspheres, comprising:

[0026] dissolving sodium alginate, fucoidan and the extract from the root of Psoralea corylifolia in a solvent to obtain a mixed solution;

[0027] The mixed solution is sprayed into the curing agent solution to obtain microspheres, and the microspheres are collected and freeze-dried to obtain the seaweed composite microspheres for moistening the intestine and facilitating bowel movements.

[0028] In some embodiments of the present invention, the concentrations of the mixed solution and the curing agent solution are the same, both of which are 0.5-3 wt %.

[0029] In some embodiments of the present invention, the solvents of the mixed solution and the curing agent solution are both water;

[0030] In some embodiments of the present invention, the mixed solution is sprayed into the curing agent solution at a rate of 500-600 μL / min.

[0031] In some embodiments of the present invention, the solidifying agent includes one of calcium gluconate, calcium chloride and calcium sulfate.

[0032] In some embodiments of the present invention, the method for preparing the extract from the root of Psoralea thornii comprises the following steps:

[0033] Wash and chop the roots of the prickly knotweed, add them to water and boil;

[0034] After decoction, the residue is removed from the medicinal liquid to obtain an extract;

[0035] The extract is pre-frozen and then freeze-dried to obtain the Psoralea corylifolia root extract.

[0036] In some embodiments of the present invention, during the decoction process, the amount of water added is 5-8 times the weight of the roots of the Psoralea corylifolia.

[0037] In some embodiments of the present invention, the decocting step is boiling followed by decocting for 20-50 minutes.

[0038] The third aspect of the present invention provides a use of the above-mentioned intestinal moistening and laxative seaweed composite microspheres in the preparation of any one or more of the following products (1)-(7);

[0039] (1) Products that help to moisten the intestines and promote bowel movements;

[0040] (2) Products that help relieve constipation;

[0041] (3) Products that help reduce the duration of constipation;

[0042] (4) Products that help increase bowel movement volume;

[0043] (5) Products that help increase the water content of feces;

[0044] (6) Products that help promote intestinal motility;

[0045] (7) Products that help prevent constipation.

[0046] In some embodiments of the present invention, the products include medicines, functional foods and health products.

[0047] A fourth aspect of the present invention provides a product that helps to moisten the intestines and promote bowel movements, comprising the above-mentioned seaweed composite microspheres that help to moisten the intestines and promote bowel movements.

[0048] In some embodiments of the present invention, the product is a medicine, a functional food or a health product.

[0049] The beneficial effects of the present invention are:

[0050] The seaweed composite microspheres for moistening the intestine and relieving constipation provided by the present invention can moisten the intestine and relieve constipation, quickly change the symptoms of constipation, are safe and have no toxic side effects, and can be used for a long time. It has been verified that the seaweed composite microspheres for moistening the intestine and relieving constipation provided by the present invention can take effect within 3-4 hours of consumption, increase the amount of defecation, increase the water content of feces, increase the intestinal propulsion rate, promote intestinal peristalsis, prevent and relieve constipation, and have no obvious adverse reactions. And it can be eaten as an oral granule. When the granule is taken with water, a suspension with excellent taste can be formed. When taken with water, the seaweed composite microspheres for moistening the intestine and relieving constipation can be quickly dispersed in water to form a uniform suspension, with a viscosity similar to porridge, but without the viscosity of polysaccharides, and a smooth mouthfeel. And the ideal taste can be achieved without long-term stirring, suitable for long-term use, and improved public acceptance, more suitable for a fast-paced lifestyle. DETAILED DESCRIPTION

[0051] Since common constipation treatment drugs are highly irritating, they are difficult to use in special populations such as the elderly, pregnant women, and children. Long-term use may cause side effects or dependence, and patients have a poor user experience, and they cannot fundamentally solve the problem.

[0052] In view of the above problems, the present invention provides a laxative seaweed composite microsphere made of fucoidan, sodium alginate and Psoralea spinulosa extract for moistening the intestine.

[0053] In order to enable those skilled in the art to more clearly understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below in conjunction with specific embodiments.

[0054] Example 1

[0055] A method for extracting active ingredients of traditional Chinese medicine from the root of Psoralea corylifolia comprises the following steps:

[0056] Raw material preparation: Clean the roots of the thorny knot and remove dirt and impurities. Cut the cleaned roots of the thorny knot into pieces of appropriate size to increase the contact area with water, improve the extraction efficiency, and facilitate subsequent processing.

[0057] Extraction: Put 300g of root fragments of the prickly pear into a decoction pot, add an appropriate amount of deionized water, the amount of water is 7 times the weight of the medicinal material, heat to boiling and then simmer on low heat for 40 minutes. Stir constantly during the period to prevent the medicinal material from being scorched and ensure that the effective ingredients are fully released.

[0058] Filtration: Filter the boiled medicinal liquid to remove the residue and obtain the extract.

[0059] Freeze drying: Transfer the extract of the root of the prickly knotweed into a clean glass dish and place it in a -40°C refrigerator for pre-freezing for at least 24 hours. Transfer the pre-frozen extract of the root of the prickly knotweed into a freeze dryer for freeze drying.

[0060] The freeze-drying procedure is as follows:

[0061] a. Pre-freezing stage: Place the product in a freeze-drying chamber, set the pre-freezing temperature to -40°C, and the vacuum degree to 0 mbar for 2 hours to ensure that the product is completely frozen. The purpose of this stage is to form uniform ice crystals to prepare for subsequent sublimation drying.

[0062] b. Sublimation drying stage:

[0063] Temperature -35°C, vacuum 25 mbar, for 1 hour.

[0064] Temperature -30°C, vacuum 25 mbar, for 1 hour.

[0065] Temperature -20°C, vacuum 25 mbar, duration 3 hours 30 minutes.

[0066] Temperature -10°C, vacuum 25 mbar, duration 3 hours 30 minutes.

[0067] The temperature was 0°C and the vacuum was 25 mbar for 3 hours. Then the temperature was increased by 10°C every three hours to promote the sublimation of ice and remove most of the free water.

[0068] c. Final drying and storage: Keep at 20°C and 25 mbar vacuum for 8 hours to ensure that the product is completely dry. After freeze-drying, it should be immediately collected and sealed in a dry container to prevent the product from absorbing moisture or oxidation.

[0069] The Herba Lysimachiae extracts used in the following examples are all Herba Lysimachiae extracts prepared in Example 1.

[0070] Example 2

[0071] A method for preparing seaweed composite microspheres for moistening the intestine and relieving constipation:

[0072] 1. Raw material preparation: weigh 6 grams of sodium alginate, 3 grams of fucoidan, and 2 grams of Psoralea corylifolia extract, mix well and set aside.

[0073] 2. Microsphere preparation:

[0074] a. Preparation of sodium alginate mixture solution: Weigh 5 g of the mixture obtained in step 1 and dissolve it in 500 mL of deionized water and stir to prepare a solution with a mass concentration of 1%.

[0075] b. Prepare curing agent solution: weigh 3g calcium gluconate and dissolve it in 300mL deionized water, stirring until the calcium gluconate is completely dissolved.

[0076] c. Set up the coaxial airflow injection system: Fix the coaxial airflow injection system, put the sodium alginate mixed solution into the syringe, connect it to the inner tube of the coaxial nozzle, and connect the nitrogen to the outer tube of the coaxial nozzle.

[0077] d. Solidification of microspheres: Transfer the calcium gluconate solution to an open glass dish, turn on the coaxial airflow injection system, and spray the sodium alginate mixture solution evenly into the calcium gluconate solution below. Adjust the flow rate of the sodium alginate mixture solution to about 500 μL / min, and adjust the gas pressure to about 0.3 MPa to generate a stable gas shear force to ensure the uniformity and morphology of the microspheres.

[0078] e. Collect microspheres: Transfer the solution containing microspheres in step d to a centrifuge tube. Centrifuge at 1000 rpm for 10 min. After centrifugation, carefully pour out the supernatant and keep the microspheres at the bottom of the centrifuge tube. Add deionized water and shake, then centrifuge again. Repeat the above steps 2-3 times. Wash away the unreacted sodium alginate and calcium ions.

[0079] 3. Freeze-dried storage: After the last centrifugation in step 2 above, remove the supernatant, transfer to a clean glass dish, and place in a -40°C refrigerator for pre-freezing for at least 24 hours. Transfer the pre-frozen microspheres to a freeze dryer for freeze drying. The particle size of the laxative seaweed composite microspheres obtained in Example 2 is between 90-200 μm.

[0080] The freeze-drying procedure is as follows:

[0081] a. Pre-freezing stage: Place the product in a freeze-drying chamber, set the pre-freezing temperature to -40°C, and the vacuum degree to 0 mbar for 2 hours to ensure that the product is completely frozen. The purpose of this stage is to form uniform ice crystals to prepare for subsequent sublimation drying.

[0082] b. Sublimation drying stage:

[0083] Temperature -35°C, vacuum 25 mbar, for 1 hour.

[0084] Temperature -30°C, vacuum 25 mbar, for 1 hour.

[0085] Temperature -20°C, vacuum 25 mbar, duration 3 hours 30 minutes.

[0086] Temperature -10°C, vacuum 25 mbar, duration 3 hours 30 minutes.

[0087] The temperature was 0°C and the vacuum was 25 mbar for 3 hours. Then the temperature was increased by 10°C every three hours to promote the sublimation of ice and remove most of the free water.

[0088] c. Final drying and storage: Keep at 20°C and 25 mbar vacuum for 8 hours to ensure that the product is completely dry. After freeze-drying is completed, it should be immediately collected and sealed in a dry container to prevent the product from absorbing moisture or oxidation.

[0089] Example 3

[0090] A method for preparing seaweed composite microspheres for moistening the intestine and relieving constipation:

[0091] 1. Raw material preparation: weigh 5 grams of sodium alginate, 3 grams of fucoidan, and 2 grams of prickly pear extract, mix well and set aside.

[0092] 2. Microsphere preparation:

[0093] a. Prepare a sodium alginate mixture solution: weigh 5 g of the mixture obtained in step 1 into a clean beaker, and add 500 mL of deionized water to prepare a solution with a mass concentration of 1%.

[0094] b. Prepare curing agent solution: weigh 2g calcium gluconate into a beaker, add 200mL deionized water, and stir until the calcium gluconate is completely dissolved.

[0095] c. Set up the coaxial airflow injection system: Fix the coaxial airflow injection system, put the sodium alginate mixed solution into the syringe, connect it to the inner tube of the coaxial nozzle, and connect the nitrogen to the outer tube of the coaxial nozzle.

[0096] d. Solidification of microspheres: Transfer the calcium gluconate solution to an open glass dish, turn on the coaxial airflow injection system, and evenly spray the sodium alginate mixture solution into the calcium gluconate solution below. Adjust the flow rate of the sodium alginate mixture solution to about 450 μL / min, and adjust the gas pressure to about 0.35 MPa to generate a stable gas shear force to ensure the uniformity and morphology of the microspheres.

[0097] e. Collect microspheres: Transfer the solution containing microspheres in step d to a centrifuge tube. Centrifuge at 800 rpm for 10 min. After centrifugation, carefully pour out the supernatant and retain the microspheres at the bottom of the centrifuge tube. Add deionized water and shake, then centrifuge again. Repeat the above steps 2-3 times to wash away the unreacted sodium alginate and calcium ions.

[0098] 3. Freeze-dried storage: After the last centrifugation in step 2 above, remove the supernatant, transfer to a clean glass dish, and place in a -40°C refrigerator for pre-freezing for at least 24 hours. Transfer the pre-frozen microspheres to a freeze dryer for freeze drying. The particle size of the laxative seaweed composite microspheres obtained in Example 3 is between 90-200 μm.

[0099] The freeze-drying procedure is as follows:

[0100] a. Pre-freezing stage: Place the product in a freeze-drying chamber, set the pre-freezing temperature to -40°C, and the vacuum degree to 0 mbar for 2 hours to ensure that the product is completely frozen. The purpose of this stage is to form uniform ice crystals to prepare for subsequent sublimation drying.

[0101] b. Sublimation drying stage:

[0102] Temperature -35°C, vacuum 25 mbar, for 1 hour.

[0103] Temperature -30°C, vacuum 25 mbar, for 1 hour.

[0104] Temperature -20°C, vacuum 25 mbar, duration 3 hours 30 minutes.

[0105] Temperature -10°C, vacuum 25 mbar, duration 3 hours 30 minutes.

[0106] The temperature was 0°C and the vacuum was 25 mbar for 3 hours. Then the temperature was increased by 10°C every three hours to promote the sublimation of ice and remove most of the free water.

[0107] c. Final drying and storage: Keep at 20°C and 25 mbar vacuum for 8 hours to ensure that the product is completely dry. After freeze-drying, it should be immediately collected and sealed in a dry container to prevent the product from absorbing moisture or oxidation.

[0108] Example 4

[0109] A method for preparing seaweed composite microspheres for moistening the intestine and relieving constipation:

[0110] 1. Raw material preparation: weigh 6 grams of sodium alginate, 2 grams of fucoidan, and 2 grams of prickly pear extract, mix well and set aside.

[0111] 2. Microsphere preparation:

[0112] a. Prepare a sodium alginate mixture solution: weigh 5 g of the mixture obtained in step 1 into a clean beaker, and add 500 mL of deionized water to prepare a solution with a mass concentration of 1%.

[0113] b. Prepare curing agent solution: weigh 3g of calcium gluconate into a beaker, add 300mL of deionized water, and stir until the calcium gluconate is completely dissolved.

[0114] c. Set up the coaxial airflow injection system: Fix the coaxial airflow injection system, put the sodium alginate mixed solution into the syringe, connect it to the inner tube of the coaxial nozzle, and connect the nitrogen to the outer tube of the coaxial nozzle.

[0115] d. Solidification of microspheres: Transfer the calcium gluconate solution to an open glass dish, turn on the coaxial airflow injection system, and evenly spray the sodium alginate mixture solution into the calcium gluconate solution below. Adjust the flow rate of the sodium alginate mixture solution to about 550 μL / min, and adjust the gas pressure to about 0.30 MPa to generate a stable gas shear force to ensure the uniformity and morphology of the microspheres.

[0116] e. Collect microspheres: Transfer the solution containing microspheres in step d to a centrifuge tube. Centrifuge at 1100 rpm for 6 minutes. After centrifugation, carefully pour out the supernatant and retain the microspheres at the bottom of the centrifuge tube. Add deionized water and shake, then centrifuge again. Repeat the above steps 2-3 times. Wash away the unreacted sodium alginate and calcium ions.

[0117] 3. Freeze-dried storage: After the last centrifugation in step 2 above, remove the supernatant, transfer to a clean glass dish, and place in a -40°C refrigerator for pre-freezing for at least 24 hours. Transfer the pre-frozen microspheres to a freeze dryer for freeze drying. The particle size of the laxative seaweed composite microspheres obtained in Example 4 is between 90-200 μm.

[0118] The freeze-drying procedure is as follows:

[0119] a. Pre-freezing stage: Place the product in a freeze-drying chamber and set the pre-freezing temperature to -40°C for 2 hours to ensure that the product is completely frozen. The purpose of this stage is to form uniform ice crystals to prepare for subsequent sublimation drying.

[0120] b. Sublimation drying stage:

[0121] Temperature -35°C, vacuum 25 mbar, for 1 hour.

[0122] Temperature -30°C, vacuum 25 mbar, for 1 hour.

[0123] Temperature -20°C, vacuum 25 mbar, duration 3 hours 30 minutes.

[0124] Temperature -10°C, vacuum 25 mbar, duration 3 hours 30 minutes.

[0125] The temperature was 0°C and the vacuum was 25 mbar for 3 hours. Then the temperature was increased by 10°C every three hours to promote the sublimation of ice and remove most of the free water.

[0126] c. Final drying and storage: Keep at 20°C and 25 mbar vacuum for 8 hours to ensure that the product is completely dry. After freeze-drying, it should be immediately collected and sealed in a dry container to prevent the product from absorbing moisture or oxidation.

[0127] Example 5

[0128] The above-mentioned seaweed composite microspheres for moistening the intestine and relieving constipation are used as granules for moistening the intestine and relieving constipation.

[0129] 6 g of the laxative seaweed composite microspheres prepared in Examples 2-4 of the present invention were respectively placed in 150 mL of warm water and gently stirred for 5-10 seconds to form a viscous suspension having a taste similar to porridge, but without any sticky feeling of polysaccharides, with a smooth taste and easy to take.

[0130] Example 6

[0131] The above-mentioned seaweed composite microspheres for moistening the intestine and relieving constipation are used as granules for moistening the intestine and relieving constipation.

[0132] 10 g of the laxative seaweed composite microspheres prepared in Examples 2-4 of the present invention were respectively placed in 200 mL of warm water and gently stirred for 5-10 seconds to form a viscous suspension having a taste similar to porridge, but without any sticky feeling of polysaccharides, with a smooth taste and easy to take.

[0133] Example 7

[0134] Experimental study on the functional effect of seaweed composite microspheres in moistening the intestine and relieving constipation

[0135] Modeling: Loperamide was used to establish the constipation model in mice. The mice in the normal control group were gavaged with pure water, and the mice in the other experimental groups were gavaged with loperamide to establish the constipation model in mice.

[0136] Drug preparation: The microsphere preparation method in Example 2 was used to prepare freeze-dried microspheres of sodium alginate, sodium alginate + fucoidan (weight ratio 6:4), and sodium alginate + Psoralea spinulosa extract (weight ratio 9:1) for use.

[0137] Administration: Three days after modeling, mice were given the laxative seaweed composite microspheres, during which the modeling conditions remained unchanged. The laxative seaweed composite microspheres in Example 2, as well as sodium alginate microspheres, sodium alginate + fucoidan microspheres, and sodium alginate + prickly pimple extract microspheres were prepared into solutions, and the sodium alginate microsphere group (135 mg / kg), sodium alginate + fucoidan microsphere group (135 mg / kg), sodium alginate + prickly pimple extract microsphere group (135 mg / kg), seaweed composite microsphere low-dose group (67.5 mg / kg), seaweed composite microsphere medium-dose group (135 mg / kg), seaweed composite microsphere high-dose group (270 mg / kg), and positive control group (Ma Ren Run Chang Wan 180 mg / mL) were gavaged to mice, and the gavage volume was 0.1 mL / 10 g, and the blank control group mice were gavaged with an equal amount of pure water. Continuous administration for 7 days. Drugs were administered orally with India ink (mixed with an equal volume of pure water or drug).

[0138] Data collection: After oral administration, the first black stool time and the number of stool particles of each mouse were observed for 6 hours, and the feces of each group of mice were collected and weighed within 6 hours. The feces of the mice were then placed in a 60℃ oven and dried for 12 hours before being weighed. The feces weight at this time was taken as the dry weight of the feces; the water content of the feces of each group of mice was calculated.

[0139] The calculation formula is: feces moisture content (%) = [(wet weight - dry weight) / wet weight] × 100%

[0140] Small intestinal propulsion experiment: After the collection and measurement of mice in each group were completed on the 7th day after administration, the mice were fasted but not watered for 16 hours. On the eighth day, the mice in each group were administered normally, and then each group of mice was given ink prepared with toner powder as an intestinal motility indicator. After 25 minutes, the mice were immediately killed by dislocating the cervical vertebra, the abdominal cavity was opened and the mesentery was separated, and the intestinal tube from the pylorus at the upper end and the ileocecal part at the lower end were cut and placed on a tray. The small intestine was gently straightened into a straight line, and the length of the intestinal tube was measured as the total length of the small intestine. The length from the pylorus to the front of the ink was the ink propulsion length, and the small intestinal propulsion rate was calculated.

[0141] The calculation formula is: Small intestine propulsion rate (%) = [ink propulsion length (cm) / total small intestine length (cm)] × 100%

[0142] Experimental Results

[0143] After intragastric administration, the number of feces particles, fresh feces weight, feces water content and small intestinal propulsion rate of the mice were observed within 6 hours. The results are as follows:

[0144] Table 1 Experimental results of the effect of algae composite microspheres on the gastrointestinal tract of mice

[0145]

[0146] Experimental conclusion

[0147] The experimental results in Table 1 show that the constipation model of mice was successfully established using loperamide, and there were significant differences between the model group and the blank control group. The fecal water content of the experimental group increased significantly compared with the model group, the defecation quality of the sodium alginate group increased within 6 hours, and the defecation quality of the sodium alginate + fucoidan group and the sodium alginate + thorny knot extract group increased within 6 hours compared with the sodium alginate group. Compared with the model group, the defecation quality of mice increased significantly, and the first defecation time was shortened. The addition of fucoidan and thorny knot extract can better improve the symptoms of constipation.

[0148] The time to first black stool was significantly shortened in the medium-dose group and high-dose group of seaweed composite microspheres. The number of stool particles increased within 6 hours in the low-, medium-, and high-dose groups of seaweed composite microspheres, the stool quality increased significantly, and the water content of feces increased significantly in 6 hours. The fecal quality and fecal water content of the medium-dose group of seaweed composite microspheres increased significantly. Compared with the mice in the model group, the mice in the seaweed composite microsphere group had more normal hair, better mental state, and softer feces.

[0149] As the concentration of the seaweed composite microsphere solution increased, the intestinal propulsion rate of mice increased. Compared with the model group, the positive control group had an increased small intestinal propulsion rate of mice, which was stronger than the low-dose group, equivalent to the medium-dose group, and lower than the high-dose group. The experimental results showed that the seaweed composite microspheres can increase the intestinal propulsion rate of mice and promote intestinal peristalsis.

[0150] The experimental results show that the seaweed composite microspheres provided by the present invention have the effect of moistening the intestine and relieving constipation without obvious adverse reactions, and have good function of preventing and relieving constipation.

[0151] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A seaweed composite microsphere for moistening the intestine and relieving constipation, characterized in that: Calculated by weight, it comprises 5-10 parts of sodium alginate, 1-5 parts of fucoidan, and 1-2 parts of Psoralea corylifolia root extract.

2. The intestinal moistening and laxative seaweed composite microspheres according to claim 1, characterized in that: The invention comprises 5-6 parts of sodium alginate, 3-4 parts of fucoidan and 1-2 parts of extract from the root of Psoralea corylifolia.

3. The intestinal moistening and laxative seaweed composite microspheres according to claim 1, characterized in that: It includes 6 parts of sodium alginate, 3 parts of fucoidan, and 2 parts of Psoralea corylifolia root extract; Or, it comprises 5 parts of sodium alginate, 3 parts of fucoidan, and 2 parts of extract from the root of Psoralea corylifolia; Or, it comprises 6 parts of sodium alginate, 2 parts of fucoidan, and 2 parts of Psoralea corylifolia root extract.

4. The intestinal moistening and laxative seaweed composite microspheres according to any one of claims 1 to 3, characterized in that: The extract of the root of Psoralea corylifolia is obtained by decocting and freeze-drying the root of Psoralea corylifolia.

5. The intestinal moistening and laxative seaweed composite microspheres according to any one of claims 1 to 3, characterized in that: The particle size of the intestinal moistening and laxative seaweed composite microspheres is 90-200 μm.

6. A method for preparing the intestinal moistening and laxative seaweed composite microspheres according to claim 1, characterized in that: include: dissolving sodium alginate, fucoidan and the extract from the root of Psoralea corylifolia in a solvent to obtain a mixed solution; The mixed solution is sprayed into the curing agent solution to obtain microspheres, and the microspheres are collected and freeze-dried to obtain the seaweed composite microspheres for moistening the intestine and facilitating bowel movements.

7. The preparation method according to claim 6, characterized in that: The concentrations of the mixed solution and the curing agent solution are the same, both of which are 0.5-3wt%; Preferably, the solvents of the mixed solution and the curing agent solution are both water; Preferably, the mixed solution is sprayed into the curing agent solution at a rate of 500-600 μL / min; Preferably, the curing agent includes one of calcium gluconate, calcium chloride and calcium sulfate.

8. The preparation method according to claim 6, characterized in that: The preparation method of the extract from the root of Psoralea corylifolia comprises the following steps: Wash and chop the roots of the prickly knotweed, add them to water and boil; After decoction, the residue is removed from the medicinal liquid to obtain an extract; The extract is pre-frozen and then freeze-dried to obtain the root extract of Psoralea corylifolia; Preferably, during the decoction process, the amount of water added is 5-8 times the weight of the root of Psoralea corylifolia; Preferably, the decocting step is boiling and then decocting for 20-50 minutes.

9. Use of the intestinal moistening and laxative seaweed composite microspheres according to claim 1 in the preparation of any one or more of the following products (1) to (7); (1) Products that help to moisten the intestines and promote bowel movements; (2) Products that help relieve constipation; (3) Products that help reduce the duration of constipation; (4) Products that help increase bowel movement volume; (5) Products that help increase the water content of feces; (6) Products that help promote intestinal motility; (7) Products that help prevent constipation; Preferably, the products include medicines, functional foods and health products.

10. A product that helps to moisten the intestines and promote bowel movements, characterized in that: The seaweed composite microspheres for moistening the intestine and relieving constipation as claimed in claim 1; Preferably, the product is a medicine, a functional food or a health product.

Images