Whitening composition containing oxyresveratrol microcapsules as well as preparation method and application of whitening composition

Abstract

The invention discloses a whitening composition containing oxyresveratrol microcapsules as well as a preparation method and application of the whitening composition, and relates to the technical field of cosmetics. The invention provides a whitening composition. The whitening composition is prepared from the following components in parts by weight: 1 to 20 parts of oxyresveratrol microcapsules, 0.01 to 2 parts of gentiana rigescens extract, 0.1 to 10 parts of verbascos extract and 0.01 to 1 part of lupin extract, wherein the oxidized resveratrol microcapsule comprises a core material, a wall material, carrier oil and an emulsifier, and the weight ratio of the core material to the wall material to the carrier oil to the emulsifier is 1: (1-6): (1-8): (0.05-0.2). According to the oxidized resveratrol micro-capsule with the specific structure, active ingredients can penetrate into the deep layer of skin more deeply through a slow release mechanism, and by matching with other components, visible whitening and repairing effects can be achieved within a short time, and discomfort caused by a powerful whitening agent is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, in particular to a whitening composition containing oxidized resveratrol microcapsules and a preparation method and application thereof. Background Art

[0002] Pigmented dark circles are mainly caused by pigmentation, which is common in people with darker skin and sensitive skin. Staying up late, prolonged exposure to sunlight or skin allergies can cause chronic inflammation of the skin, leading to post-inflammatory pigmentation. The skin around the eyes is especially thinner and more susceptible to external stimuli such as allergies and friction, so pigmentation is more likely to occur around the eyes. Existing technologies for pigmented dark circles usually use ingredients such as vitamin C, arbutin and niacinamide. These ingredients have whitening effects and can effectively reduce pigmentation. However, vitamin C is unstable and may lose its activity during use; arbutin may irritate sensitive skin at high concentrations; niacinamide has low irritation, but the whitening effect is average and the effect is too slow.

[0003] Therefore, how to balance the whitening efficacy with the stability and mildness of the formula is the key to lightening pigmented dark circles. Summary of the invention

[0004] Based on this, the purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide a whitening composition containing oxidized resveratrol microcapsules and a preparation method and application thereof.

[0005] To achieve the above-mentioned purpose, the technical scheme adopted by the present invention is: a whitening composition, comprising the following components in parts by weight: 1-20 parts of oxidized resveratrol microcapsules, 0.01-2 parts of gentiana yunnanensis extract, 0.1-10 parts of mullein extract, and 0.01-1 parts of lupin extract; wherein the oxidized resveratrol microcapsules include a core material, a wall material, a carrier oil and an emulsifier, and the weight ratio of the core material, the wall material, the carrier oil and the emulsifier is core material: wall material: carrier oil: emulsifier = 1: (1-6): (1-8): (0.05-0.2); the core material is oxidized resveratrol; the wall material is a mixture of cerium oxide and β-glucan, and the weight ratio of cerium oxide to β-glucan is 1: (0.5-5); the carrier oil is a mixture of juniper fruit oil and sunflower seed oil.

[0006] The main active ingredient of Yunnan Gentian extract is gentiopicroside, which has significant anti-inflammatory, antioxidant and repairing effects, and has a good soothing effect on the dark circles caused by vascular inflammation; the mullein extract is rich in flavonoids, such as quercetin, flavonols, etc., which have whitening and soothing effects through anti-oxidation, anti-inflammatory and inhibition of tyrosinase activity; the lupin extract is rich in isoflavones and peptide compounds, which have antioxidant, lightening and repairing effects, help brighten the skin tone and enhance the skin barrier function. The above three extracts, in conjunction with the oxidized resveratrol microcapsules of the specific structure provided by the present invention, the whitening composition of the four ingredients, comprehensively enhance the whitening and repairing effects, and play a role in inhibiting post-inflammatory pigmentation and strengthening the fragile skin around the eyes. The whitening composition provided by the present invention can effectively lighten pigmented dark circles, and is mild and non-irritating to the eyes, and is suitable for people with sensitive skin.

[0007] Oxidized resveratrol has antioxidant, anti-inflammatory, photoprotective and whitening effects, but its stability is poor and it is easily affected by light, heat and oxygen. The present invention uses a microcapsule technology with a specific structure to effectively protect its activity, control release and increase its stability. Cerium oxide is an inorganic substance with good stability and safety, and has strong antioxidant and self-repairing properties. It can remove free radicals through catalytic reactions and play a protective role in microcapsules; β-glucan is a water-soluble polysaccharide with certain viscosity and adhesion, and has good biocompatibility, moisture retention and immunomodulatory effects. β-glucan can provide good encapsulation and biocompatibility. Cerium oxide combines with β-glucan by physical adsorption to form the wall layer of the microcapsule. The particles of cerium oxide are harder, which may affect the encapsulation effect of β-glucan. If the two are not suitable in the ratio, the stability and release characteristics of the microcapsule may be affected. The inventors have experimentally found that the weight ratio of cerium oxide and β-glucan is within the range specified in this application to achieve good stability and release characteristics.

[0008] Juniper berry oil is rich in unsaturated fatty acids and vitamin E, has the ability to promote skin regeneration and repair, and is suitable for repairing damaged skin. It also promotes proteasome activity, inhibits lipofuscin deposition, improves internal turbidity of cells, and has the effect of brightening and making the skin transparent. Sunflower seed oil is rich in vegetable oleic acid, which can help repair the skin barrier, improve skin hydration, and keep the skin soft and smooth. It also has certain anti-inflammatory properties, which helps reduce skin redness and inflammation, and is suitable for sensitive skin. The inventors found through experiments that when Juniper berry oil and sunflower seed oil are used together as carrier oils for oxidized resveratrol microcapsules, the oxidized resveratrol microcapsules have better stability and release characteristics.

[0009] In the preparation process of oxidized resveratrol microcapsules, the components and dosage of the emulsifier are not further explored, and any emulsifier commonly used in the art can be used; preferably, the emulsifier includes at least one of polyethylene glycol-100, steareth-2, steareth-21, oleth-10, glyceryl oleate, sorbitan stearate, polysorbate-60, methyl glucose sesquistearate, caprylic capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetrastearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate.

[0010] Preferably, the whitening composition comprises the following components in parts by weight: 5-15 parts of oxidized resveratrol microcapsules, 0.1-0.5 parts of Gentiana yunnanensis extract, 1-5 parts of Verbascum extract, and 0.1-0.5 parts of Lupinus officinalis extract.

[0011] Preferably, the mass percentage of oxidized resveratrol microcapsules in the whitening composition is 50-80%.

[0012] The inventors found in actual experiments that the proportion of oxidized resveratrol microcapsules, Gentiana yunnanensis extract, Verbascum extract, and Lupinus odoratus extract in the whitening composition will affect the whitening and repairing effect. When it is within the above range, the whitening and repairing effect is better.

[0013] Preferably, the weight ratio of the core material, wall material, carrier oil and emulsifier is core material: wall material: carrier oil: emulsifier = 1: (2-4): (2-5): (0.05-0.2).

[0014] Preferably, the weight ratio of cerium oxide to β-glucan = 1:(1-3);

[0015] And / or, the weight ratio of the juniper berry oil to the sunflower seed oil = 1: (0.5-8); preferably, the weight ratio of the juniper berry oil to the sunflower seed oil = 1: (3-4).

[0016] The inventors found in actual experiments that the weight ratio of the core material, the wall material and the carrier oil, the weight ratio of cerium oxide and β-glucan, and the weight ratio of juniper fruit oil and sunflower seed oil will affect the stability and release characteristics of the finally prepared oxidized resveratrol microcapsules, thereby affecting the whitening and repairing effect of the whitening composition. When within the above specific weight ratio range, the stability and release characteristics of the oxidized resveratrol microcapsules are better.

[0017] Preferably, the β-glucan includes at least one of β-1,3 / α-1,3-glucan, β-1,3-glucan, β-1,4-glucan, β-1,6-glucan, and β-1,3(1,6)-glucan, and the molecular weight of the β-glucan is 1 million to 5 million Daltons.

[0018] Preferably, the average particle size of the cerium oxide is 0.05-10 μm; more preferably, the average particle size of the cerium oxide is 0.2-3 μm.

[0019] The inventors found in actual experiments that the average particle size of cerium oxide affects the stability and release characteristics of the finally prepared oxidized resveratrol microcapsules. When the average particle size of cerium oxide is 0.2-3 μm, the stability and release characteristics of the oxidized resveratrol microcapsules are better.

[0020] Preferably, the method for preparing the oxidized resveratrol microcapsules comprises the following steps:

[0021] (1) adding water at 30-40° C. to the wall material and stirring to dissolve, thereby obtaining a mixture A;

[0022] (2) mixing the carrier oil and the emulsifier, heating and homogenizing to obtain a mixture B;

[0023] (3) mixing oxidized resveratrol and a solvent evenly, adding mixture B, and homogenizing to obtain mixture C;

[0024] (4) The mixture C and the mixture A are heated and homogenized, spray-dried, and collected and sieved to obtain the oxidized resveratrol microcapsules.

[0025] Preferably, in step (1), the weight ratio of the wall material to water is 1:(1-2); ultrasonic treatment is used to ensure that the material is completely dissolved or evenly dispersed.

[0026] Preferably, in step (2), the mixture is heated to 55-65° C., the homogenization speed is 1000-1300 rpm, and the homogenization time is 3-5 min.

[0027] Preferably, in step (3), the solvent is anhydrous ethanol, and the mass volume ratio of oxidized resveratrol to the solvent is 1 g: (5-6) mL; the speed of the homogenizer is 1000-1300 rpm, and the homogenization time is 3-5 min.

[0028] Preferably, in step (4), the temperature is heated to 60-70° C., the speed of homogenization is 12000-20000 rpm, and the homogenization time is 20-40 min.

[0029] Preferably, in step (3), the air inlet temperature of the spray drying tower is 120°C-150°C, and the outlet temperature is 70°C-80°C. The microcapsule powder is collected by a collection device (such as an air flow separator) after spray drying. Then, a sieving method is used to remove large particles and irregular particles to obtain the desired oxidized resveratrol microcapsules.

[0030] In addition, the present invention provides application of the whitening composition in preparing skin products.

[0031] Furthermore, the present invention provides a skin product, which comprises the whitening composition.

[0032] Preferably, the skin product further comprises an auxiliary material, and the auxiliary material is at least one of a moisturizer, a thickener, a pH adjuster, an emulsifier, a preservative, a flavor, and deionized water.

[0033] Preferably, the skin product comprises one of lotion, emulsion, cream, eye cream, eye mask, essence and spray.

[0034] Optionally, the present invention provides an eye cream comprising the following components in percentage by mass: 1-15% whitening composition, 0.05-0.5% thickener, 0.5-2% moisturizer, 5-20% emulsifier, 0.5-3% preservative, 0.01-0.3% pH adjuster, and the balance deionized water.

[0035] Exemplarily, the moisturizer includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, glycerol, budding septin polysaccharide, and ceramide; the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol; the emulsifier includes caprylic capric triglyceride, C14-22 alcohol, C12 The invention relates to a novel polysaccharide comprising: a polysaccharide comprising: a polysaccharide of at least one of -20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetraisopalmitate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate; the preservative comprises at least one of 1,3-propylene glycol, 1,2-propylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, and acrylates / C10-30 alkyl acrylate crosspolymer; and the pH adjuster comprises at least one of arginine, tromethamine, and EDTA-disodium.

[0036] Compared with the prior art, the present invention has the following beneficial effects: (1) The gentian extract, mullein extract, lupin extract and the oxidized resveratrol microcapsules of the specific structure provided by the present invention work together to form a whitening composition with four ingredients, which comprehensively enhances the whitening and repairing effects, inhibits post-inflammatory pigmentation, and strengthens the fragile skin around the eyes. The whitening composition provided by the present invention can effectively reduce pigmented dark circles, is mild and non-irritating to the eyes, and is suitable for people with sensitive skin. (2) The oxidized resveratrol microcapsules of the specific structure provided by the present invention can allow the active ingredients to penetrate deeper into the deep layers of the skin through a slow release mechanism, ensuring that they exert the best effect, rather than high-concentration stimulation in a short period of time. Compared with traditional methods, due to the mild formula and complementary ingredients, visible whitening and repairing effects can be achieved in a shorter time, and the discomfort caused by strong whitening agents can be reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 These are pictures of the eye conditions of volunteers after using the eye creams of the blank application example and application example 1; among them, (a) is a picture of the eye condition of the volunteer before use, (b) is a picture of the eye condition of the volunteer after using it for 14 days, and (c) is a picture of the eye condition of the volunteer after using it for 28 days. DETAILED DESCRIPTION

[0038] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are the same batch of raw materials.

[0039] The raw materials used in the present invention are further described:

[0040] Gentiana yunnanensis extract: purchased from Yunnan Yingge Biotechnology Co., Ltd.;

[0041] Mullein extract: purchased from Phenbiox, Italy;

[0042] Lupin extract: purchased from Coyenne, France;

[0043] Safflower extract: purchased from Guangzhou Meiyi Biotechnology Co., Ltd.;

[0044] Cerium oxide-1: purchased from Beijing Weiye Innovation Technology Co., Ltd., the trade name is luminescent TM , average particle size 200nm;

[0045] Cerium oxide-2: purchased from Qinghe County Tenghui Metal Materials Co., Ltd., product number HT-CeO2-2, average particle size 1 μm;

[0046] Cerium oxide-3: purchased from Shandong Desheng New Materials Co., Ltd., with an average particle size of 10 μm;

[0047] Cerium oxide-4: purchased from Qinghe County Tenghui Metal Materials Co., Ltd., product number HT-CeO2-1, average particle size 50nm;

[0048] β-Glucan-1: purchased from Sichuan Hetai Xinguang Biotechnology Co., Ltd., the structure is β-1,3 / α-1,3-glucan, and the molecular weight is 2 million to 2.5 million Daltons;

[0049] β-Glucan-2: purchased from BASF, Germany, with a structure of β-1,3(1,6)-glucan and a molecular weight of 4.5 million to 5 million Daltons;

[0050] Juniperus chinensis fruit oil: purchased from Jiangxi Senhai Vegetable Oil Co., Ltd.;

[0051] Sunflower oil: purchased from Croda, UK;

[0052] Oxidized resveratrol: purchased from Shaanxi Hongpeptide Biotechnology Co., Ltd.;

[0053] Oxidized Resveratrol Microcapsules-1

[0054] The preparation method of the oxidized resveratrol microcapsule-1 comprises the following steps:

[0055] (1) adding water at 35° C. to the wall material, with the weight ratio of the wall material to the water being 1:2, and using ultrasonic treatment to ensure that the material is completely dissolved, thereby obtaining a mixture A;

[0056] (2) mixing the carrier oil and the emulsifier, heating to 60° C., and homogenizing at a homogenizing speed of 1000 rpm for 3 min to obtain a mixture B;

[0057] (3) mixing oxidized resveratrol and ethanol evenly, wherein the mass volume ratio of oxidized resveratrol to ethanol is 1 g:6 mL; adding mixture B, and homogenizing to obtain mixture C; the homogenizing speed is 1000 rpm, and the homogenizing time is 5 min;

[0058] (4) The mixture C and the mixture A are heated to 60° C., homogenized at a speed of 12000 rpm for 40 min, and spray dried at an air inlet temperature of 120° C. and an outlet temperature of 80° C. The microcapsule powder is collected by a collection device (air flow separator) after spray drying. A centrifugal sieve is then used to remove large particles and irregular particles to obtain the desired oxidized resveratrol microcapsules.

[0059] The oxidized resveratrol microcapsule-1 comprises 1 part of core material, 2 parts of wall material, 5 parts of carrier oil, and 0.1 parts of emulsifier; the core material is oxidized resveratrol; the wall material is a mixture of cerium oxide-1 and β-glucan-1, with a weight ratio of 1:1; the carrier oil is a mixture of juniper fruit oil and sunflower seed oil, with a weight ratio of 1:3; the emulsifier is polyethylene glycol-100;

[0060] Oxidized resveratrol microcapsule-2~Oxidized resveratrol microcapsule-21

[0061] The preparation method is exactly the same as that of the oxidized resveratrol microcapsule-1, except that the wall material and carrier oil of the oxidized resveratrol microcapsule are different, as shown in Table 1.

[0062] Among them, in oxidized resveratrol microcapsules-19, only the type of wall material is different, and only β-glucan-1 is included.

[0063] In the oxidized resveratrol microcapsule-20, only the type of wall material is different. The wall material is a mixture of β-cyclodextrin, chitosan, gum arabic, and maltodextrin, all of which are commercially available products, maltodextrin: chitosan: gum arabic: β-cyclodextrin = 2:1:3:1.

[0064] In oxidized resveratrol microcapsules-21, only the type of carrier oil is different, containing only sunflower oil.

[0065] Table 1

[0066]

[0067]

[0068]

[0069] Examples and Comparative Examples

[0070] The present application provides a whitening composition, the components and weight portions of the whitening composition are shown in Table 2-3, and the preparation method of the whitening composition is: weigh oxidized resveratrol microcapsules, gentiana yunnanensis extract, mullein extract, and lupinus extract according to weight portions, and do not add relevant components if there are no relevant components, and mix them evenly to obtain the whitening composition.

[0071] Table 2

[0072]

[0073]

[0074]

[0075] Table 3

[0076]

[0077]

[0078] Application Examples

[0079] The application examples of the present invention provide an eye cream, the components (mass percentage) of the eye cream are as shown in Table 4; wherein the compositions in application examples 1-19 respectively use the whitening compositions prepared in embodiments 1-19; the whitening compositions in comparative application examples 1-14 respectively use the whitening compositions prepared in comparative examples 1-14; for example, the whitening composition used in application example 1 is the whitening composition in embodiment 1, the whitening composition used in application example 5 is the whitening composition in embodiment 5, the whitening composition used in application example 15 is the whitening composition in embodiment 15, and the same applies to the others; the whitening composition used in application example 20 is the whitening composition prepared in embodiment 1.

[0080] Table 4

[0081]

[0082] The preparation method of the eye cream provided in Application Example 1 comprises the following steps:

[0083] (1) Mix the humectant and thickener with water, heat to 85±2° C., homogenize at 1200 rpm for 4 min, and keep warm for later use to obtain prefabricated component A;

[0084] (2) Mix the emulsifier, heat to 85±2° C., homogenize at 1200 rpm for 4 min, and keep warm for later use to obtain prefabricated component B;

[0085] (3) Mix the preservatives and heat to 60±2°C to melt to obtain a prefabricated C component;

[0086] (4) Heat the preformed component A to 80±2°C, add the preformed component B at a speed of 250 rpm, stir and mix, then cool to 60±2°C, add the preformed component C at a speed of 250 rpm, stir and mix, then cool to 40°C, add the whitening composition and continue stirring for 8 minutes, finally add the remaining pH adjuster to adjust the pH to 6.0, then stop stirring, discharge, and obtain the eye cream.

[0087] The preparation methods of the remaining application examples are the same as that of application example 1, except that no relevant components are added.

[0088] Performance test-1 Loading rate and encapsulation rate test

[0089] The present invention explores the encapsulation efficiency and loading rate of oxidized resveratrol microcapsules 1-21, and specifically includes the following steps:

[0090] 1. Loading rate: Weigh 50.0 mg of oxidized resveratrol microcapsules, and record the mass as m t , add 20 mL of methanol, extract by ultrasonic at 20 ° C for 20 min, then centrifuge to obtain the supernatant, the speed is 8000 rpm, the supernatant is fixed to 25 mL, and the mass of oxidized resveratrol is calculated by high performance liquid chromatography quantitative detection, recorded as m o ; Then calculate the load rate according to the formula, load rate = (m o / m t )×100%, and record the calculated load rate in Table 5;

[0091] 2. Encapsulation rate: According to the above loading rate test method, m o ; Weigh 50.0 mg of oxidized resveratrol microcapsules and add them to 20 mL of methanol. Vortex extract at 20°C for 10 seconds, then centrifuge to obtain the supernatant at a speed of 8000 rpm. The supernatant is fixed to 25 mL. HPLC quantitative detection is used to calculate the mass of oxidized resveratrol, which is recorded as m s Then the encapsulation efficiency was calculated according to the formula: Encapsulation efficiency = (m o -m s ) / m o × 100%, and the calculated encapsulation efficiency is recorded in Table 5.

[0092] Table 5

[0093]

[0094]

[0095] As can be seen from the above table, the oxidized resveratrol microcapsules prepared by the present invention through the selection of specific wall materials and specific carrier oils have a loading rate of more than 10.5% and an encapsulation rate of more than 90%.

[0096] By comparing oxidized resveratrol microcapsules 1-4 and oxidized resveratrol microcapsules 15-16, it can be seen that the weight ratio of the core material, wall material and carrier oil in the preparation process of oxidized resveratrol microcapsules will affect the loading rate and encapsulation rate of the oxidized resveratrol microcapsules. When the weight ratio of the core material, wall material and carrier oil is core material: wall material: carrier oil = 1: (2-4): (2-5), the loading rate and encapsulation rate are higher.

[0097] From the comparison of oxidized resveratrol microcapsules 1, oxidized resveratrol microcapsules 5-7, and oxidized resveratrol microcapsules 17-18, it can be seen that when the weight ratio of cerium oxide to β-glucan in the wall material is 1:(1-3), the loading rate and encapsulation rate are higher.

[0098] From the comparison between oxidized resveratrol microcapsules 1 and oxidized resveratrol microcapsules 8-10, it can be seen that when the weight ratio of the juniper fruit oil to the sunflower seed oil is 1:(3-4), the loading rate and encapsulation rate are higher.

[0099] From the comparison between oxidized resveratrol microcapsules 1 and oxidized resveratrol microcapsules 11-14, it can be seen that the type of wall material will affect the loading rate and encapsulation rate. When the average particle size of cerium oxide is 0.2-3 μm, the loading rate and encapsulation rate are higher.

[0100] Performance Test-2 Stability

[0101] The present invention explores the stability of oxidized resveratrol microcapsules 1-21, and specifically includes the following steps:

[0102] Weigh 3 portions of oxidized resveratrol microcapsules 1-21 respectively and spread them on a surface dish, 2 g each, and place them in an environment of 4°C, 25°C and 50°C, respectively, with humidity controlled at 65% and 15W light on. Then, the mass of oxidized resveratrol was tested on days 0 and 28. T0 、m T28 , the detection method is the same as m o The retention rate of oxidized resveratrol was calculated as follows: T28 / m T0 ×100%; the test results for 28 days are shown in Table 6;

[0103] Table 6

[0104]

[0105] As can be seen from the above table, the oxidized resveratrol microcapsules prepared by the present invention through the selection of specific wall materials and specific carrier oils have a retention rate of more than 80% for oxidized resveratrol at 4°C, 25°C and 50°C, and can maintain good stability.

[0106] By comparing oxidized resveratrol microcapsules 1-4 and oxidized resveratrol microcapsules 15-16, it can be seen that the weight ratio of the core material, wall material and carrier oil in the preparation process of oxidized resveratrol microcapsules will affect the retention rate of the oxidized resveratrol microcapsules. When the weight ratio of the core material, wall material and carrier oil is core material: wall material: carrier oil = 1: (2-4): (2-5), the retention rate of oxidized resveratrol is higher and the stability is better.

[0107] From the comparison of oxidized resveratrol microcapsules 1, oxidized resveratrol microcapsules 5-7, and oxidized resveratrol microcapsules 17-18, it can be seen that when the weight ratio of cerium oxide to β-glucan in the wall material is 1:(1-3), the retention rate of oxidized resveratrol is higher and the stability is better.

[0108] From the comparison between oxidized resveratrol microcapsules 1 and oxidized resveratrol microcapsules 8-10, it can be seen that when the weight ratio of the juniper fruit oil to the sunflower seed oil is 1:(3-4), the retention rate of oxidized resveratrol is higher and the stability is better.

[0109] From the comparison between oxidized resveratrol microcapsules 1 and oxidized resveratrol microcapsules 11-14, it can be seen that the type of wall material will affect the retention rate. When the average particle size of cerium oxide is 0.2-3 μm, the retention rate of oxidized resveratrol is higher and the stability is better.

[0110] Performance Test-3 Skin Penetration Test

[0111] The present invention investigates the skin permeability of oxidized resveratrol microcapsules 1-21, and specifically comprises the following steps:

[0112] Experimental method: In vitro pig skin test was used, and the exposed skin area in the diffusion cell was 1.5 cm 2 , the volume of the receiving chamber is 5.0mL. A phosphate buffer solution with a pH of 5.8 and anhydrous ethanol in a volume ratio of 50:50 was used as the receiving solution. Weigh the oxidized resveratrol microcapsules 1-21 respectively, add an appropriate amount of pure water, and then stir at room temperature to prepare a 1% w / v corresponding emulsion at a speed of 600rpm, then take 200μL of the corresponding emulsion and apply it evenly on the skin, and cover with a film to prevent evaporation. The transdermal absorption of oxidized resveratrol at different time points was tested by HPLC, and the time to reach the maximum cumulative penetration (in h) and the maximum cumulative penetration (in μg / cm 2 The longer the time to reach the maximum cumulative permeation amount, the slower the release of the microcapsule; the larger the maximum cumulative permeation amount, the better the skin permeability of the microcapsule. The results are shown in Table 7 below.

[0113] Table 7

[0114]

[0115]

[0116] It can be seen from the above table that the oxidized resveratrol microcapsules prepared by selecting specific wall materials and specific carrier oils in the present invention can achieve a good sustained release effect and have better skin permeability.

[0117] By comparing oxidized resveratrol microcapsules 1-4 and oxidized resveratrol microcapsules 15-16, it can be seen that the weight ratio of the core material, wall material and carrier oil in the preparation process of oxidized resveratrol microcapsules will affect the sustained release effect and skin permeability of the oxidized resveratrol microcapsules. When the weight ratio of the core material, wall material and carrier oil is core material: wall material: carrier oil = 1: (2-4): (2-5), the sustained release effect and skin permeability of the oxidized resveratrol microcapsules are better.

[0118] From the comparison of oxidized resveratrol microcapsules 1, oxidized resveratrol microcapsules 5-7, and oxidized resveratrol microcapsules 17-18, it can be seen that when the weight ratio of cerium oxide to β-glucan in the wall material is 1:(1-3), the sustained release effect and skin permeability of the oxidized resveratrol microcapsules are better.

[0119] From the comparison between oxidized resveratrol microcapsules 1 and oxidized resveratrol microcapsules 8-10, it can be seen that when the weight ratio of the juniper fruit oil to the sunflower seed oil is 1:(3-4), the sustained release effect and skin permeability of the oxidized resveratrol microcapsules are better.

[0120] From the comparison between oxidized resveratrol microcapsules 1 and oxidized resveratrol microcapsules 11-14, it can be seen that the type of wall material will affect the sustained release effect and skin permeability. When the average particle size of cerium oxide is 0.2-3 μm, the sustained release effect and skin permeability of oxidized resveratrol microcapsules are better.

[0121] Performance test-4 melanin inhibition test

[0122] Experimental method: The cell line used was mouse melanoma cell B16F10 (Wuhan Saios Biotechnology Co., Ltd.), and the test conditions were: incubator temperature 37±1°C, saturated humidity, carbon dioxide 5±1%; cell culture and treatment were performed according to grouping, followed by testing. The specific test was the determination of cell melanin content, and the test method was as follows:

[0123] (1) The cell suspension was inoculated into a 6-well plate at a culture density of 1×10 6 / mL, cultured in DMEM medium (Gibco) for 24 hours;

[0124] (2) The blank control group was added with DMEM culture medium containing 0.1% of blank application example eye cream, and the sample groups were added with DMEM culture medium containing 0.1% of application example and comparative application example eye cream, respectively, and the cells were collected after culturing for 24 hours;

[0125] (3) Determination of cell melanin content: The cells were lysed at 80°C, and then the total amount of melanin in each lysate was measured at a wavelength of 405 nm using an ELISA reader. The melanin content (OD value) was calculated and adjusted based on the total protein concentration, and the melanin content of the blank group (solvent control) was normalized and compared with the sample group. The melanin content inhibition rate of each embodiment and comparative example was calculated using the following formula:

[0126] Melanin content inhibition rate (%) = (blank group OD value - sample OD value) / blank group OD value * 100%.

[0127] The results are shown in Table 8.

[0128] Table 8

[0129]

[0130]

[0131] It can be seen from the above table that the eye cream prepared by the application example of the present invention has a melanin content inhibition rate of more than 60%, and has a good effect of lightening pigmented dark circles.

[0132] Performance Test-5 Human skin patch test.

[0133] 30 volunteers were recruited, 15 males and 15 females, aged 20-50 years old. The closed patch test method was used. An equal amount (0.020mL-0.025mL) of the test sample (eye cream prepared by the application example, comparative application example and blank application example) was placed in a specific patch tester, and it was applied to the volunteer's arm with a low-allergenic tape, and gently pressed to evenly apply it on the skin for 24 hours; the blank control group was distilled water, and the blank application example was an eye cream without a whitening composition. After 24 hours, the patch tester was removed, and the skin reaction was observed after 0.5 hours, 24 hours, and 48 hours, and the results were recorded. The level of adverse reactions to the skin is shown in Table 9 below.

[0134] Table 9

[0135]

[0136] After testing, the eye creams prepared by the application examples of the present invention, the comparative application examples and the blank application examples all showed negative reactions after human patch tests, and were safe and non-irritating to human skin.

[0137] Performance test-6 human efficacy tests on lightening, repairing and moisturizing

[0138] Experimental method: Asian adult test subjects aged 28-60 with sensitive skin and pigmented dark circles were selected and randomly divided into 35 groups, with 5 people in each group. The volunteers used samples (eye creams prepared by application examples 1-20, comparative application examples 1-14 and blank application examples) around the eyes once in the morning and evening every day, and data were collected on the 0th day (T0), 14th day (T14) and 28th day (T28) of use;

[0139] After the visit, the volunteers washed their faces with facial cleanser and sat quietly in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 30 minutes; the eyes were photographed and analyzed using VISIA-CR, the melanin content MI value of the dark circles was measured using the melanin skin detector Mexameter, and the transepidermal water loss value TEWL of the skin under the eyes was measured using the skin water loss test probe TewaMeter.

[0140] The ability to reduce pigmented dark circles is represented by the improvement in the MI value, and the ability to repair the skin barrier and moisturize is represented by the improvement in the TEWL value. The formula is as follows:

[0141] 14-day improvement rate = (T0-T14) / T0*100%;

[0142] 28-day improvement rate = (T0-T28) / T0*100%;

[0143] The results obtained are shown in Table 10;

[0144] Table 10

[0145]

[0146]

[0147] It can be seen from the above table that the eye cream prepared by the application example of the present invention has an MI value improvement rate of more than 10% and a TEWL value improvement rate of more than 15% after 28 days of use. The oxidized resveratrol microcapsules with a specific structure provided by the present invention, in synergy with the gentiana yunnanensis extract, the mullein extract, and the lupinus extract, can allow the active ingredients to penetrate deeper into the skin through a slow release mechanism, thereby comprehensively enhancing the whitening and repairing effects, and inhibiting post-inflammatory pigmentation and strengthening the fragile skin around the eyes.

[0148] Figure 1 These are pictures of the eye conditions of volunteers after using the eye creams of the blank application example and application example 1; among them, (a) is a picture of the eye condition of the volunteer before use, (b) is a picture of the eye condition of the volunteer after using it for 14 days, and (c) is a picture of the eye condition of the volunteer after using it for 28 days.

[0149] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A whitening composition, characterized in that: The invention comprises the following components in parts by weight: 1-20 parts of oxidized resveratrol microcapsules, 0.01-2 parts of gentiana yunnanensis extract, 0.1-10 parts of mullein extract, and 0.01-1 parts of lupinus extract; The oxidized resveratrol microcapsules include a core material, a wall material, a carrier oil and an emulsifier, wherein the weight ratio of the core material, the wall material, the carrier oil and the emulsifier is core material: wall material: carrier oil: emulsifier = 1: (1-6): (1-8): (0.05-0.2); the core material is oxidized resveratrol; the wall material is a mixture of cerium oxide and β-glucan, and the weight ratio of cerium oxide to β-glucan is 1: (0.5-5); the carrier oil is a mixture of juniper fruit oil and sunflower seed oil.

2. The whitening composition according to claim 1, characterized in that The invention comprises the following components in parts by weight: 5-15 parts of oxidized resveratrol microcapsules, 0.1-0.5 parts of gentiana yunnanensis extract, 1-5 parts of verbabasin extract and 0.1-0.5 parts of lupinus extract.

3. The whitening composition according to claim 1, characterized in that The mass percentage of oxidized resveratrol microcapsules in the whitening composition is 50-80%.

4. The whitening composition according to claim 1, characterized in that The weight ratio of the core material, wall material, carrier oil and emulsifier is core material: wall material: carrier oil: emulsifier = 1: (2-4): (2-5): (0.05-0.2).

5. The whitening composition according to claim 1, characterized in that The weight ratio of cerium oxide to β-glucan = 1: (1-3); And / or, the weight ratio of the juniper berry oil to the sunflower seed oil is 1:(0.5-8).

6. The whitening composition according to claim 1, characterized in that The average particle size of the cerium oxide is 0.05-10 μm.

7. The whitening composition according to claim 1, characterized in that The method for preparing oxidized resveratrol microcapsules comprises the following steps: (1) adding water at 30-40° C. to the wall material and stirring to dissolve, thereby obtaining a mixture A; (2) mixing the carrier oil and the emulsifier, heating and homogenizing to obtain a mixture B; (3) mixing oxidized resveratrol and a solvent evenly, adding mixture B, and homogenizing to obtain mixture C; (4) The mixture C and the mixture A are heated and homogenized, spray-dried, and collected and sieved to obtain the oxidized resveratrol microcapsules.

8. Use of the whitening composition according to any one of claims 1 to 7 in the preparation of skin products.

9. A skin care product, characterized in that: The skin product comprises the whitening composition according to any one of claims 1 to 7.

10. The skin care product according to claim 9, wherein: The skin products include one of lotion, emulsion, cream, eye cream, eye mask, essence and spray.

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