Anti-aging composition and skin care product for repairing staying up late injury and regulating pressure and preparation method of anti-aging composition and skin care product

Abstract

The invention relates to an anti-aging composition for repairing staying up late injury and regulating pressure, a skin care product and a preparation method of the anti-aging composition and the skin care product. The anti-aging composition and the skin care product are prepared from lavandula officinalis extract, silybum marianum extract and rice fermentation liquor according to the mass ratio of (0.1-5): (0.01-2.75): (0.1-18). Compared with the prior art, through the synergistic effect of the lavandula angustifolia extract, the silybum marianum extract and the rice fermentation liquor, cell metabolism is accelerated by regulating pressure, and endogenous aging is retarded; by preventing light aging caused by ultraviolet rays and slowing down exogenous aging caused by environmental factors, endogenous aging and exogenous aging are slowed down at the same time through the synergistic effect of the three components.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to an anti-aging composition for repairing damage caused by staying up late and regulating stress, a skin care product and a preparation method thereof. Background Art

[0002] Aging is not only a complex and continuous biological process, but also an inevitable natural law of the human body, characterized by changes in cells and molecules, a gradual decrease in the body's ability to maintain balance, and increased cell apoptosis and aging. The two processes of skin aging are mainly endogenous aging and exogenous aging. Endogenous aging is controlled by personal genetics and gradually appears over time, while exogenous aging is mainly caused by environmental factors, such as ultraviolet radiation, smoking, wind, sun exposure, and exposure to harmful chemicals.

[0003] There have been many studies and applications in the field of skin anti-aging, but there are few studies on endogenous aging caused by stress, such as patent CN202310659694.9, which mainly achieves anti-aging by inhibiting exogenous aging factors such as ultraviolet damage. Stress is not only an important factor in modern life, but also an important factor affecting endogenous aging. Long-term stress will accelerate collagen degradation, induce skin inflammatory response, and cause signs of aging on the skin.

[0004] The increasingly fast-paced modern life has given people a deeper understanding of stress. Studies have shown that stress can have a profound impact on the skin. Under stress, the release of substance P and adrenaline increases, thereby activating inflammatory markers and causing the skin to be susceptible to inflammatory reactions. In addition, the body responds effectively to stress by increasing cortisol levels. Long-term stress will cause cortisol levels to continue to rise, thereby degrading collagen, accelerating skin aging, causing dull skin, loss of elasticity and increased wrinkles. Excessive cortisol levels can also affect sleep, leading to insomnia, waking up at night and other problems. Lack of sleep can weaken immune cells, reduce the body's immunity, and make the skin more susceptible to inflammatory reactions. At present, there are inventions in the field of stress regulation, such as patent CN201410453054.3, which mainly affects the sense of smell through aromatic components, plays a role in soothing emotions, reducing stress and anxiety. Because aromatic components are volatile, there are disadvantages such as a single mechanism of action, limited conditions of use, and different effects from person to person.

[0005] Melatonin, also known as the “darkness hormone”. This hormone is released at nightfall and exerts its well-known sleep-promoting function by interacting with the suprachiasmatic nucleus of the hypothalamus and synchronizing the circadian rhythm. In addition, melatonin has multiple functions, including protective effects against skin light. Incubation of HaCaT cells with melatonin before UV irradiation can reduce cell apoptosis and promote cell viability, especially by maintaining mitochondrial membrane potential and inhibiting the activation of caspases 9, 3, and 7. Melatonin has been reported to protect DNA from damage and prevent UV-mediated reduction of antioxidant enzymes (catalase, superoxide dismutase, and glutathione peroxidase) defense. In addition, some studies have shown that melatonin has other important roles in skin processes, including regulating barrier function by increasing keratinocyte proliferation and the expression of epidermal macromolecules (such as involucrin, keratin-10 and 14), reducing skin melanin production, etc.

[0006] At present, there are studies on the application of melatonin in cosmetics, such as patent CN202010069646.0, which mainly repairs the skin by adding melatonin to the composition. Melatonin plays an important role in regulating the human body's biological clock and promoting sleep. However, there are certain safety risks in using cosmetics containing melatonin. Long-term excessive intake of melatonin will disrupt the balance of hormones in the human body and lead to a series of physiological disorders.

[0007] To sum up, there are currently no good anti-aging skin care products that can deal with the damage caused by staying up late and regulate stress at the same time. Summary of the invention

[0008] The purpose of the present invention is to overcome the defects of the above-mentioned prior art and provide an anti-aging composition for repairing damage caused by staying up late and regulating stress and a preparation method thereof.

[0009] The purpose of the present invention can be achieved by the following technical scheme: an anti-aging composition for repairing damage caused by staying up late and regulating stress, comprising lavender extract, milk thistle extract and rice fermentation liquid in a mass ratio of (0.1-5): (0.01-2.75): (0.1-18).

[0010] Furthermore, the mass ratio of the lavender extract, the milk thistle extract and the rice fermentation liquid is: (0.1-3): (0.1-2.5): (0.1-15).

[0011] The present invention also provides a skin care product containing the anti-aging composition for repairing damage caused by staying up late and regulating pressure. The weight percentage of the anti-aging composition in the skin care product is 0.21-25%.

[0012] Furthermore, the weight percentage of the anti-aging composition in the skin care product is 0.3-22.5%.

[0013] Furthermore, the skin care product also includes auxiliary materials, and the auxiliary materials include thickeners, skin conditioners, moisturizers, pH adjusters, preservatives and fragrances.

[0014] Further, the thickener is selected from: one or more of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, polyacrylamide, xanthan gum, propylene glycol, behenyl alcohol, carbomer, laureth-7, cetyl alcohol, stearyl alcohol and hydroxyethyl cellulose;

[0015] The skin conditioning agent is selected from one or more of isohexadecane, olive oil, sodium hyaluronate, lauryl alcohol, avocado butter, allantoin, butylene glycol, squalane, panthenol, lactic acid, tocopherol and dipotassium glycyrrhizinate;

[0016] The moisturizing agent is selected from: one or more of hyaluronic acid, allantoin, glycerin, ceramide, sodium lactate, butylene glycol, ethylhexylglycerin, hydrogenated grape seed oil, olive oil and shea butter;

[0017] The pH regulator is one or more of arginine and lactic acid;

[0018] The aromatic agent includes essence;

[0019] The preservative is selected from: one or more of p-hydroxyacetophenone, chlorphenesin, ethylhexylglycerin, and pentylene glycol.

[0020] Furthermore, the skin care products include lotions, creams, freeze-dried powders, skin care water and essences.

[0021] Furthermore, the essence comprises the following components in percentage by weight:

[0022] Water balance;

[0023] Xanthan gum 0.01-5;

[0024] Sodium hyaluronate 0.01-1;

[0025] Allantoin 0.1-10;

[0026] Glycerol 1-15;

[0027] Butanediol 1-10;

[0028] Cetyl alcohol 0.1-10;

[0029] Stearyl alcohol 0.1-10;

[0030] Arginine 0.1-5;

[0031] Essence 0.1-2;

[0032] Chlorphenesin 0.05-0.5;

[0033] Lavender extract 0.1-5;

[0034] Milk thistle extract 0.1-2.75;

[0035] Rice fermentation liquid 0.1-18.

[0036] The present invention also provides a method for preparing the essence containing the anti-aging composition for repairing damage caused by staying up late and regulating stress, comprising the following steps:

[0037] a. Aqueous phase: fully wet and mix xanthan gum, sodium hyaluronate, allantoin, glycerin and butylene glycol, add them into water, stir thoroughly until completely dissolved, and heat to 75-80℃;

[0038] b. Oil phase: Heat the raw materials cetyl alcohol and stearyl alcohol in a water bath to 75-80°C and stir until completely dissolved;

[0039] c. Emulsification: Add the oil phase obtained in step b to the aqueous phase obtained in step a and stir until homogenous;

[0040] d. Add arginine to the product obtained in step c, stir homogenously, and cool to 40-50 ° C with stirring;

[0041] e. Add essence and chlorphenesin and stir until homogenous;

[0042] f. After the temperature drops to 35-38℃, add lavender extract, milk thistle extract and rice fermentation liquid, stir and homogenize, filter and sterilize before filling, test the bacterial content and conduct quality control tests on toxins, immunogenicity, etc. The essence is obtained after passing the test. This essence can repair the damage caused by staying up late and regulate the impact of stress on the skin. It is not only suitable for the general population, but also for the post-aesthetic relief of sensitive skin.

[0043] Furthermore, the stirring and homogenizing in steps c to f is: stirring at 200-400 rpm for 3-5 minutes and then homogenizing at 2000-4000 rpm for 3-5 minutes.

[0044] Compared with the prior art, the present invention has the following beneficial effects:

[0045] 1. The present invention uses lavender extract, milk thistle extract, and rice fermentation liquid to synergistically regulate pressure, accelerate cell metabolism, and slow down endogenous aging; it prevents light aging caused by ultraviolet rays and slows down exogenous aging caused by environmental factors. The three work synergistically to slow down endogenous aging and exogenous aging at the same time. Compared with the existing invention, the mechanism of action is more perfect.

[0046] 2. The present invention improves skin condition by increasing melatonin expression in cells, improving circadian rhythm, improving sleep quality, and relieving stress. Compared with the existing invention, the safety risks caused by excessive melatonin intake are avoided and the application scope of the composition is increased.

[0047] 3. The present invention soothes emotions and relieves stress by increasing the expression of melatonin in cells and synergizing with aromatic ingredients. Compared with the invention that only regulates stress through aromatic ingredients, the mechanism of action is more comprehensive, and through the synergistic penetration-enhancing effect of rice fermentation liquid, the permeability of the effective ingredients is increased, the inactivation of the ingredients caused by the volatilization of the effective ingredients is avoided, and the stability and bioavailability of the composition are improved. DETAILED DESCRIPTION

[0048] The present invention is described in detail below with reference to specific embodiments.

[0049] The present invention selects a specific ratio of lavender extract, milk thistle extract, and rice fermentation liquid to be used in combination in skin care products, which has a synergistic anti-aging effect, regulates pressure, accelerates cell metabolism, slows down endogenous aging; prevents photoaging caused by ultraviolet rays, slows down exogenous aging caused by environmental factors, and improves the skin from both endogenous and exogenous sources. The specific description is as follows:

[0050] 1. Lavender

[0051] Lavender (lavandula hybrida) extract is an ingredient extracted from hybrid lavender plants. It can reduce skin inflammation and irritation, help balance oil secretion, and has antibacterial and antioxidant effects. Studies have shown that lavender extract can reduce the levels of pro-inflammatory cytokines such as TNF-α, interleukin-6 (IL-6), and interleukin-1β (IL-1β), block the activation of nuclear transcription factor Kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) signaling pathways, and can also upregulate the expression of TGF-β1, epidermal growth factor (EGF), and fibroblast growth factor-2 (FGF-2), increase fibroblast activity, and induce the expression of type I and type III collagen, thereby playing an anti-aging role. In particular, lavender extract can increase the level of melatonin in human epidermal keratinocytes (NHEK). Melatonin interacts with clock genes by affecting negative regulatory factors Period (Per1 / 2 / 3), Crytochrome (Cry1 / 2) and positive regulatory factors Bmal1 and Clock (Ko 2006), helping to regulate the phase of the circadian rhythm and maintain the stability of the rhythm. In addition, melatonin can work together with aromatic ingredients in the extract, such as linalool and linalyl acetate, to calm anxiety and improve sleep quality, thereby reducing the damage caused by stress to the skin.

[0052] 2. Milk Thistle

[0053] Milk thistle (silybum marianum) is an annual or biennial herb of the genus Silybum in the Asteraceae family. Milk thistle extract can selectively remove senescent cells by inducing apoptosis, inhibit the expression of p-H2AX and p21, and restore senescent cells to youth and transform them into cells with young characteristics. Experiments have shown that milk thistle extract can reduce the generation of reactive oxygen species (ROS) caused by UVA and UVB stimulation, reduce caspase-3 and its activity, and reduce the levels of matrix metalloproteinases (MMP-1) and heat shock proteins (HSP 70), thereby playing a photoprotective role. In addition, it has strong free radical scavenging and antioxidant abilities, and can inhibit the activity of collagenase and elastase, slowing down the decomposition of collagen, thereby playing an anti-aging role. Milk thistle extract has the same antioxidant activity and anti-aging effects as chemical retinol, and is much less irritating than retinol and its derivatives.

[0054] 3. Rice fermentation liquid

[0055] Rice fermentation liquid is an ingredient made by the precise fermentation of the yeast species Saccharomyces Veronae and Akita Komachi rice grown at 40 degrees north latitude. It contains a variety of effective ingredients such as amino acids, sugars, fruit acids, polyamines, nucleic acids, etc. Among them, α-kg can directly act on the cell energy cycle, promote the mRNA expression of filaggrin, involucrin and serine palmitoyltransferase, increase the cell energy factor ATP, accelerate cell metabolism, and increase the construction of epidermal cells and the increase of natural moisturizing factors, thereby delaying endogenous aging. Studies have shown that rice fermentation liquid has a penetration-promoting effect, which can increase the penetration of other effective ingredients in the skin, improve bioavailability, and achieve better results when used in synergy.

[0056] 4. Synergy

[0057] The two processes of skin aging are mainly endogenous aging and exogenous aging. The present invention uses lavender extract, milk thistle extract, and rice fermentation liquid to work together to regulate pressure, accelerate cell metabolism, and slow down endogenous aging; it prevents light aging caused by ultraviolet rays and slows down exogenous aging caused by environmental factors. The three work together to slow down endogenous aging and exogenous aging at the same time.

[0058] The following is a detailed description through specific examples. The raw materials and detection methods involved in the present invention are commonly used raw materials and detection methods in the art unless otherwise specified.

[0059] The present invention uses essence as an example to set up each group of experiments, but is not limited to the use of essence. The composition can also be applied to various skin care products such as lotions, creams, freeze-dried powders, and skin care water. The essence is the above key combination and a thickener, a chelating agent, and a pH regulator. Preferably, the raw materials for preparing the pressure-adjusting anti-aging essence also include any one or a combination of at least two of a preservative and a flavor.

[0060] Examples 1-3 and Comparative Examples 1-7

[0061] (1) The formulations of the embodiments and comparative examples are shown in Table 1 below:

[0062] Table 1 Formulations of Examples 1-3 and Comparative Examples 1-7

[0063] .

[0065] The difference between Example 2-3 and Example 1 is only that each active ingredient is reduced.

[0066] The difference between Control Examples 1-3 and Example 3 is the lack of one key composition component, the difference between Control Examples 4-6 and Example 3 is the lack of two key composition components, and the difference between Control Example 7 and Example 3 is that no composition component is added.

[0067] The preparation processes of the embodiments and the comparative examples are the same, which are as follows:

[0068] (2) Preparation process of essence:

[0069] a. Water phase: Place water in a container with a stirring device and stir. Fully moisten and mix the raw materials xanthan gum, sodium hyaluronate, allantoin, glycerin and butylene glycol, then add them into the water. Stir thoroughly until completely dissolved and heat to 75-80°C.

[0070] b. Oil phase: Place the raw materials cetyl alcohol and stearyl alcohol in another container, heat to 75-80°C in a water bath, and stir until completely dissolved.

[0071] c. Emulsification: Under stirring, add the oil phase mixture obtained in step b to the aqueous phase in step a, stir at 300 rpm for 5 minutes, and then homogenize at 3000 rpm for 5 minutes.

[0072] d. Add arginine to the product obtained in step c, stir at 300 rpm for 3 minutes, then homogenize at 3000 rpm for 3 minutes. Stir and cool to 45°C.

[0073] e. Add essence and chlorphenesin to the product obtained in step d, stir at 300 rpm for 3 minutes, and then homogenize at 3000 rpm for 3 minutes.

[0074] f. After cooling to 35-38°C, add lavender extract, milk thistle extract and rice fermentation liquid, stir at 200rpm for 5 minutes, and then homogenize at 2000rpm for 3 minutes. Filter and sterilize before filling, test the bacterial content, and perform quality control on toxins and immunogenicity to obtain the desired medical post-aesthetic soothing essence suitable for sensitive skin.

[0075] Efficacy evaluation test

[0076] 1. Change rate of moisture content in the stratum corneum over time and transepidermal water loss (TWEL)

[0077] Test sample: the essence obtained in Examples 1-3 and Comparative Examples 1-7;

[0078] Test equipment: TWEL( Combo), Corneometer CM825

[0079] Test method:

[0080] 1) Demographic information of subjects: A total of 40 subjects were included, all of whom were female. The average age was 38 years old, including 7 subjects aged 18-25, 7 subjects aged 26-30, 10 subjects aged 36-40, and 16 subjects aged 41-45. They were randomly divided into 10 groups, with 4 subjects in each group. The test volunteers used the sample once in the morning and evening, taking 2-5 grams each time and applying it evenly on the face, massaging for 1-2 minutes, and then wiping with a cotton towel. The data on the 0th day, the 7th day, the 14th day, and the 28th day were tested respectively.

[0081] 2) Statistics of adverse events during the test: None

[0082] 3) Skin physical and chemical data analysis results:

[0083] The calculation formula of the change rate is: Change rate = (average value of the measured values ​​after use of each group of subjects - average value of the measured values ​​before use of each group of subjects) ÷ average value of the measured values ​​before use of each group of subjects × 100%

[0084] A. Skin stratum corneum moisture content detection: Use Corneometer CM825 to detect the stratum corneum moisture content after using the sample. The higher the change value, the better the moisturizing effect.

[0085] Table 2 Changes of moisture content in the stratum corneum of the subjects over time

[0086] sample After 7 days of use After 14 days of use After 28 days of use Example 1 14.20％ 16.41％ 19.18％ Example 2 11.76％ 15.29％ 18.74％ Example 3 9.20％ 11.43％ 14.71％ Comparative Example 1 8.54％ 10.56％ 13.40％ Comparative Example 2 8.70％ 10.76％ 13.22％ Comparative Example 3 8.91％ 9.84％ 10.92％ Comparative Example 4 7.63％ 8.46％ 9.50％ Comparative Example 5 7.45％ 8.75％ 9.49％ Comparative Example 6 7.91％ 8.16％ 9.28％ Comparative Example 7 6.97％ 7.80％ 8.97％

[0087] As can be seen from Table 2 above, after using the essence, as the use time increases, the change rate of the water content of the stratum corneum of Examples 1-3 and Control Examples 1-3 increases significantly, indicating that the water content of the stratum corneum of the skin has improved and the skin barrier has been repaired. After the same use time, the change rate of the water content of the stratum corneum of Example 1 is the largest, indicating that the improvement effect of the water content of the stratum corneum of the skin after using the essence of Example 1 is more significant.

[0088] From the comparison of the results of Example 3 and Control Examples 1-7, it can be seen that compared with not using either the hydrolyzed lavender extract or the milk thistle extract, the use of both the hydrolyzed lavender extract and the milk thistle extract has the most significant effect on improving the moisture content of the stratum corneum, indicating that the essence prepared from the combination has a stronger barrier repair effect.

[0089] B.TEWL(g / m 2 / h) Transepidermal water loss test

[0090] The transepidermal water loss (TEWL) is accurately measured using the non-invasive measurement device TEWL (Dermalab Combo). If the TEWL decreases significantly before and after the use of the test product, it means that the skin barrier has been restored and the product has a repairing effect.

[0091] Table 3 Change rate of TWEL value of subjects' skin over time

[0092] sample After 7 days of use After 14 days of use After 28 days of use Example 1 -15.77％ -18.74％ -24.75％ Example 2 -14.23％ -16.74％ -21.96％ Example 3 -10.78％ -12.65％ -15.21％ Comparative Example 1 -10.23％ -11.71％ -13.86％ Comparative Example 2 -10.29％ -11.86％ -13.52％ Comparative Example 3 -10.15％ -11.57％ -13.10％ Comparative Example 4 -6.23％ -8.80％ -9.04％ Comparative Example 5 -6.41％ -8.19％ -9.42％ Comparative Example 6 -6.35％ -8.25％ -9.27％ Comparative Example 7 -2.15％ -3.57％ -4.10％

[0093] As can be seen from Table 3, the change rate of the TWEL value of the subject's skin after using Example 1 is the largest; compared with Control Examples 1-7, the change rate of the TWEL value of the subject's skin after using Example 3 is more significant, indicating that the hydrolyzed lavender extract and the milk thistle extract have a synergistic effect, and the composition has a stronger ability to reduce the TWEL value of the subject's skin. After the subject used Example 1 for 2 weeks, the TWEL value of the skin decreased significantly by 18.74%; after using the product for 4 weeks, the TWEL value decreased significantly by 24.75%. The above results indicate that under the test conditions, Example 1 can reduce the skin TWEL value to the greatest extent and has the strongest repair effect.

[0094] 2. In vitro TNF-α inflammatory factor content determination LPS-induced macrophage RAW264.7 test

[0095] Experimental model: Mouse mononuclear macrophage leukemia cell line RAW264.7

[0096] Test sample: 0.02% of the composition obtained in Application Examples 1-9, as shown in Table 4 below

[0097] Table 4 Application Examples 1-10 Formulations

[0098]

[0099]

[0100] Negative control: refers to the cell group to which only LPS is added but no test sample is added, which is used to evaluate the basal level of LPS-induced inflammatory response.

[0101] Positive control: A drug known to have anti-inflammatory activity (100 μg / mL dexamethasone was used in this experiment) was selected to act on cells together with LPS to verify the sensitivity and reliability of the experimental system.

[0102] Test method:

[0103] 1) Cytotoxicity test: Determine the most suitable sample concentration through cytotoxicity test;

[0104] 2) Cell inoculation: Mouse macrophages (RAW264.7) were cultured in DMEM high-glucose medium containing 10% fetal bovine serum albumin, and then the cells were collected with a cell scraper. The collected cells were diluted with cell culture medium to the inoculation density (the degree of confluence reached 45%-60% 24 hours after inoculation), and inoculated into 96-well plates, 200 μL per well, and cultured in a CO2 incubator for 24±2 hours;

[0105] 3) Administration: Discard the culture medium in the 96-well plate and carry out induction and administration. Add culture medium containing a certain concentration of the test substance (i.e., test sample) and LPS to the test wells, add cell culture medium containing LPS to the negative control wells, add culture medium containing the positive control sample and LPS to the positive control wells, and add cell culture medium to the blank / solvent control wells, 200 μL per well. After administration, place the 96-well plate in a CO2 incubator for 24 h ± 2 h;

[0106] 4) Collection of cell supernatant: After the incubation, collect the cell supernatant and place it in a 1.5 ml sterile centrifuge tube and store it in a -80°C refrigerator;

[0107] 5) ELISA test pre-experiment: Because the expression of TNF-α is high after LPS induction, the dilution ratio should be set before the formal test, and a preliminary experiment should be conducted to find out the dilution ratio of the ELISA test group to ensure that the ELISA test value falls within the range of the standard curve;

[0108] 6) ELISA test: Detect TNF-α according to the operating instructions of the TNF-α ELISA test kit. The calculation formula of TNF-α inhibition rate is: inhibition rate (%) = (TC / C) × 100%

[0109] Wherein, T is the average TNF-α content of the test substance, and C is the average TNF-α content of the negative control;

[0110] Table 5 Type I TNF-α content results

[0111]

[0112]

[0113] As can be seen from Table 5, compared with the negative control, the inhibition rate of TNF-α content in Application Examples 1-9 is significantly different, indicating that the samples have the ability to inhibit TNF-α content, which can further illustrate that the samples have a soothing effect, among which Application Example 1 has the strongest inhibitory effect on TNF-α; compared with Application Examples 4-9, Application Examples 1-3 have a more significant inhibitory effect on TNF-α, indicating that hydrolyzed lavender extract, milk thistle extract, and rice fermentation liquid have a synergistic effect, and the composition has a stronger ability to inhibit TNF-α.

[0114] 3. Product stability testing

[0115] Test sample: Samples obtained from Examples 1-3

[0116] Test method: internal control standard, observe the appearance color, viscosity, pH within three months. "CYCLE" refers to the cycle, and the cycle setting is: from -15℃ to 45℃, 45℃ to -15℃ for five cycles.

[0117] Table 6 Product stability test results

[0118]

[0119]

[0120] As can be seen from Table 6 above, the color, viscosity and pH of the prepared essence did not change significantly under high and low temperature conditions or light exposure within three months, indicating that the essence has good stability.

[0121] 4. Detection of melatonin expression in human epidermal keratinocytes (NHEK)

[0122] 1) Culture medium: MEM medium + 10% fetal bovine serum;

[0123] 2) Cell inoculation: NHEK cells were cultured in a 37° C., 5% CO2 incubator for 5 days; NHEK cells were replaced with a culture medium containing 0.02% of Application Example 1-10 and cultured in a culture incubator for 48 hours;

[0124] 3) Remove the supernatant and separate the cells. Wash the cells with phosphate buffered saline (PBS) solution after centrifugation and freeze at -80°C;

[0125] 4) Melatonin was extracted from the cell pellet using dichloromethane and then measured by a liquid chromatography-mass spectrometry (LC-MS / MS) system.

[0126] Table 7 Change rate of melatonin in human epidermal keratinocytes (NHEK)

[0127]

[0128]

[0129] As shown in Table 7, the expression of melatonin in Application Examples 1-3 was significantly improved. Compared with Application Examples 4-9, the expression of melatonin in Application Example 3 was more obvious, indicating that the hydrolyzed lavender extract, milk thistle extract, and rice fermentation liquid had a synergistic effect, and the composition had a stronger ability to increase the expression of melatonin.

[0130] 5. Anti-wrinkle effect evaluation

[0131] Testing instrument: Primos

[0132] Test subjects: A total of 40 subjects were included, all female. The average age was 38 years old, including 7 subjects aged 18-25, 7 subjects aged 26-30, 10 subjects aged 36-40, and 16 subjects aged 41-45. They were randomly divided into 10 groups, with 4 subjects in each group. The test volunteers used the samples (Examples 1-3 and Control Examples 1-7) once a day in the morning and evening, taking 2-5 grams each time and applying them evenly on the face, massaging for 1-2 minutes, and then wiping with a cotton towel. The data on the 0th day, the 14th day, and the 28th day were tested respectively.

[0133] Test indicators: skin wrinkle area rate, skin wrinkle Ra value, L value, ITA value (hereinafter referred to as measured values) of the test subjects at D0, D14, and D28. The wrinkle analysis value becomes smaller, indicating that skin wrinkles have improved.

[0134] The calculation formula of the change rate is: Change rate = (average value of the measured values ​​after use of each group of subjects - average value of the measured values ​​before use of each group of subjects) ÷ average value of the measured values ​​before use of each group of subjects × 100%

[0135] Table 8 Changes in skin wrinkle area and Ra value between D28 and D0

[0136]

[0137]

[0138] As shown in Table 8, the skin wrinkle area was significantly reduced after using Examples 1-3. Compared with Control Examples 1-7, the skin wrinkle area was more significantly reduced after using Example 3, indicating that the hydrolyzed lavender extract, milk thistle extract, and rice fermentation liquid have a synergistic effect, and the composition has a stronger ability to reduce the skin wrinkle area.

[0139] The invention can be understood and used by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. An anti-aging composition for repairing damage caused by staying up late and regulating stress, characterized in that: The invention comprises lavender extract, milk thistle extract and rice fermentation liquid in a mass ratio of (0.1-5):(0.01-2.75):(0.1-18).

2. The anti-aging composition for repairing damage caused by staying up late and regulating stress according to claim 1, characterized in that: The mass ratio of the lavender extract, the milk thistle extract and the rice fermentation liquid is: (0.1-3): (0.1-2.5): (0.1-15).

3. A skin care product containing the anti-aging composition for repairing damage caused by staying up late and regulating stress as claimed in claim 1 or 2, characterized in that: The anti-aging composition contained in the skin care product has a weight percentage of 0.21-25%.

4. A skin care product containing an anti-aging composition for repairing damage caused by staying up late and regulating stress according to claim 3, characterized in that: The weight percentage of the anti-aging composition in the skin care product is 0.3-22.5%.

5. A skin care product containing an anti-aging composition for repairing damage caused by staying up late and regulating stress according to claim 3, characterized in that: The skin care product also includes auxiliary materials, which include thickeners, skin conditioners, moisturizers, pH regulators, preservatives and fragrances.

6. A skin care product containing an anti-aging composition for repairing damage caused by staying up late and regulating stress according to claim 5, characterized in that: The thickener is selected from one or more of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, polyacrylamide, xanthan gum, propylene glycol, behenyl alcohol, carbomer, laureth-7, cetyl alcohol, stearyl alcohol and hydroxyethyl cellulose; The skin conditioning agent is selected from one or more of isohexadecane, olive oil, sodium hyaluronate, lauryl alcohol, avocado butter, allantoin, butylene glycol, squalane, panthenol, lactic acid, tocopherol and dipotassium glycyrrhizinate; The moisturizing agent is selected from: one or more of hyaluronic acid, allantoin, glycerin, ceramide, sodium lactate, butylene glycol, hydrogenated grape seed oil, olive oil and shea butter; The pH regulator is one or more of arginine and lactic acid; The aromatic agent includes essence; The preservative is selected from: one or more of p-hydroxyacetophenone, chlorphenesin, ethylhexylglycerin, and pentylene glycol.

7. A skin care product containing an anti-aging composition for repairing damage caused by staying up late and regulating stress according to claim 3, characterized in that: The skin care products include lotion, cream, freeze-dried powder, skin care water and essence.

8. A skin care product containing an anti-aging composition for repairing damage caused by staying up late and regulating stress according to claim 7, characterized in that: The essence comprises the following components in percentage by weight: Water balance; Xanthan gum 0.01-5; Sodium hyaluronate 0.01-1; Allantoin 0.1-10; Glycerol 1-15; Butanediol 1-10; Cetyl alcohol 0.1-10; Stearyl alcohol 0.1-10; Arginine 0.1-5; Essence 0.1-2; Chlorphenesin 0.05-0.5; Lavender extract 0.1-5; Milk thistle extract 0.1-2.75; Rice fermentation liquid 0.1-18.

9. A method for preparing the essence containing the anti-aging composition for repairing damage caused by staying up late and regulating stress as claimed in claim 8, characterized in that: The essence is prepared by the following method: a. Aqueous phase: fully wet and mix xanthan gum, sodium hyaluronate, allantoin, glycerin and butylene glycol, add them into water, stir thoroughly until completely dissolved, and heat to 75-80℃; b. Oil phase: Mix the raw materials cetyl alcohol and stearyl alcohol, heat in a water bath to 75-80°C, and stir until completely dissolved; c. Emulsification: Add the oil phase obtained in step b to the aqueous phase obtained in step a and stir until homogenous; d. Add arginine to the product obtained in step c, stir homogenously, and cool to 40-50 ° C with stirring; e. Add essence and chlorphenesin and stir until homogenous; f. After cooling to 35-38°C, add lavender extract, milk thistle extract and rice fermentation liquid, stir and homogenize; filter and sterilize, and test the bacterial content and toxin and immunogenicity to obtain the essence.

10. The method for preparing the essence containing the anti-aging composition for repairing damage caused by staying up late and regulating stress according to claim 9, characterized in that: The stirring and homogenizing in steps c to f are as follows: stirring at 200-400 rpm for 3-5 minutes and then homogenizing at 2000-4000 rpm for 3-5 minutes.

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