Ginseng essence for improving immunity and relieving fatigue and enzymolysis preparation process thereof

Abstract

The invention belongs to the technical field of ginseng fine processing, and particularly relates to ginseng essence capable of improving immunity and relieving fatigue and an enzymolysis preparation technology of the ginseng essence. According to the enzymolysis preparation process disclosed by the invention, ginseng is deeply processed mainly through key processes such as fine squeezing, ultrasonic treatment, enzymolysis, extraction and low-temperature sedimentation, and finally the ginseng essence is obtained through fine filtration and sterilization. The whole process not only ensures the safety, but also obtains rich active ingredients after detection, and has remarkable effects of resisting fatigue and improving immunity.

Description

Technical Field

[0001] The invention belongs to the technical field of ginseng fine processing, and specifically relates to a ginseng essence liquid for improving immunity and relieving fatigue and an enzymatic hydrolysis preparation process thereof. Background Art

[0002] Ginseng is a perennial herbaceous plant of the Araliaceae family. The fleshy root of ginseng is a tonic medicine, suitable for adjusting blood pressure, restoring heart function, neurasthenia and physical weakness, and also has the effects of expectoration, stomachic, diuretic and excitement. The stems, leaves, flowers, fruits and processing by-products of ginseng are all raw materials for light industry, and can be processed into products such as tobacco, wine, tea, crystal, cream and other products containing ginseng ingredients.

[0003] Ginseng has extremely high health value, but the development and extraction of ginseng are not yet in-depth. For example, the patent with publication number CN106913594A discloses a preparation process of ginseng extract, which includes the following steps: taking the discarded dregs after ginseng extraction, stirring, and then drying, the drying temperature is controlled at 30-60°C, then pouring alcohol into it for stirring and mixing, and then concentrating and drying to obtain the finished product.

[0004] The method is simple in process and cannot perform deep extraction on ginseng. Based on this, the technical solution of the present invention is proposed. Summary of the invention

[0005] In order to solve the problems existing in the prior art, the present invention provides an enzymatic preparation process of ginseng essence for improving immunity and relieving fatigue, and the enzymatic preparation process comprises the following steps: (1) washing, steam-scalding, fine-pressing and pulping fresh ginseng in sequence to obtain ginseng pulp; (2) sterilizing and ultrasonically treating the ginseng slurry in sequence to obtain a sterilized slurry; (3) adding a composite enzyme to the sterilization slurry and stirring the mixture to obtain an enzymatic slurry; (4) sequentially performing enzyme inactivation, cyclic leaching and reflux on the enzymatic hydrolysis slurry to obtain an extract; (5) The extract is allowed to settle at low temperature, and the supernatant is taken out, and then filtered and sterilized to obtain the ginseng essence for improving immunity and relieving fatigue.

[0006] Preferably, in step (1), the steaming temperature is 105-110° C., and the steaming time is 3-5 min.

[0007] Preferably, in step (1), the pulping is performed at a material-to-water ratio of 1:12-20.

[0008] Preferably, in step (2), the sterilization temperature is 95-98° C., and the sterilization time is 5-8 min.

[0009] Preferably, in step (2), the frequency of the ultrasonic treatment is 18-20 kHz, the treatment power is 500-800 W, and the treatment time is 30-35 min.

[0010] Preferably, in step (3), the complex enzyme comprises the following raw materials in parts by weight: 10-15 parts of cellulase, 10-15 parts of hemicellulase, 25-30 parts of amylase, 5-10 parts of pectinase, 20-30 parts of cellobiase, and 2-5 parts of protease; And / or, in step (3), the stirring treatment time is 16-18 hours, and the stirring treatment temperature is 55°C.

[0011] Preferably, in step (4), the enzyme inactivation temperature is 85-95°C and the enzyme inactivation time is 10-15 min; And / or, in step (4), the number of cycles of leaching and reflux is 3 to 4 times, the leaching and reflux temperature is 80 to 85° C., and the time of each leaching and reflux is 20 to 30 minutes.

[0012] Preferably, in step (5), the temperature of the low-temperature precipitation is 0-2°C, and the time of the low-temperature precipitation is 7-8h; And / or, in step (5), the sterilization temperature is 105-110° C., and the sterilization time is 40-50 min.

[0013] It should be emphasized that enzymatic hydrolysis is to decompose the macromolecular substances (such as ginsenosides, polysaccharides, etc.) in ginseng into small molecular substances to improve their bioavailability. To facilitate understanding of the present invention, the role of the relevant enzymes is explained: (i) Cellulase and pectinase: Cellulose and pectin are the main components of ginseng cell walls. Cellulase can decompose cellulose, and pectinase can hydrolyze pectin. The use of these two enzymes can destroy the cell wall structure of ginseng, making it easier to release the active ingredients in ginseng cells. When extracting ginsenosides, using cellulase and pectinase to treat ginseng raw materials can significantly increase the extraction rate of ginsenosides.

[0014] (ii) Protease: Ginseng contains a certain amount of protein, which can be decomposed into amino acids and small peptides by protease. These decomposition products may be related to the pharmacological effects of ginseng, and can also remove some impurity proteins that may affect the quality of ginseng products. Moreover, during the enzymatic hydrolysis process, protease may interact with other components in ginseng, further changing the composition and properties of ginseng extract.

[0015] (iii) Amylase: Ginseng contains a small amount of starch. Amylase can break down starch into small molecular sugars such as oligosaccharides and glucose. This helps to improve the taste and stability of ginseng extract, and in the subsequent processing, it reduces the adverse effects of starch and prevents starch aging during storage.

[0016] Based on the same technical concept, the present invention also provides a ginseng essence liquid for improving immunity and relieving fatigue obtained by the above enzymatic preparation process.

[0017] It should be further explained that the present invention has strict standards for the origin, planting, picking, and screening of ginseng, specifically: (i) Origin: The core production area of ​​Huangfengling in Changbai Mountain is the ginseng source base.

[0018] (ii) Planting: Follow the harmony of heaven, earth and people, that is, grasp the appropriate planting and growth time (day), create a superior planting environment (land), fine management and collaborative cooperation (people). Select seeds and raise seedlings in spring or autumn, transplant seedlings, grow for 5 years, and harvest in September. Cool climate (annual average temperature 15℃-25℃, annual precipitation 500~1000 mm, humus loose soil. 5-step planting management (land preparation-planting-shading-water-fertilization management).

[0019] (iii) Full-age fresh harvest: 5-year-old fresh harvest in September. 5 years old, sufficient nutrition. The harvest period is crucial. Harvesting is carried out from early September to late September. During this period, the saponin content in the ginseng root is at a relatively high level, and the effective ingredients such as ginseng polysaccharides and volatile oils also reach a relatively coordinated and appropriate proportion and content.

[0020] (iv) Screening criteria: Grading and selection. Raw ginseng is classified by specifications and grades according to the geographical indication product, Jilin Changbai Mountain ginseng. The taproot is cylindrical, white or yellowish white on the surface, free of rust and sulfur, white or light yellowish white in cross section, firm and without raw core, fragrant, sweet and slightly bitter.

[0021] The beneficial effects of the present invention are: The enzymatic preparation process of the present invention mainly processes ginseng through key processes such as fine pressing, ultrasound, enzymatic hydrolysis, extraction, and low-temperature sedimentation, and finally obtains ginseng essence after fine filtration and sterilization. The whole process not only ensures safety, but more importantly, rich active ingredients are obtained after testing, which has significant anti-fatigue and immunity-enhancing effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0023] Figure 1 This is a typical diagram of the movement trajectory of zebrafish after sample treatment (the black line is the slow movement distance, the green line is the medium-speed movement distance, and the red line is the fast movement distance).

[0024] Figure 2 This is the data graph of the total movement distance of zebrafish after sample treatment (compared with the model control group, ***p<0.001).

[0025] Figure 3 This is a data chart of the anti-fatigue efficacy of the samples.

[0026] Figure 4 This is a typical graph of the fluorescence intensity of macrophages in the tail vein of zebrafish after sample treatment in the first evaluation (green fluorescence represents macrophages, and the yellow dotted line represents the analysis site).

[0027] Figure 5 This is the fluorescence intensity data of zebrafish tail vein macrophages after sample treatment in the first evaluation (compared with the model control group, ***p < 0.001).

[0028] Figure 6 This is the first evaluation of the sample's immune-enhancing efficacy data chart.

[0029] Figure 7 This is a typical graph of the fluorescence intensity of macrophages in the tail vein of zebrafish after sample treatment in the second evaluation (green fluorescence represents macrophages, and the yellow dotted line represents the analysis site).

[0030] Figure 8 This is the fluorescence intensity data of zebrafish tail vein macrophages after sample treatment in the second evaluation (compared with the model control group, **p<0.01, ***p<0.001).

[0031] Fig. 9 This is a data chart of the sample's immune-enhancing efficacy in the second evaluation. DETAILED DESCRIPTION

[0032] To make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described in detail below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other implementation methods obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention.

[0033] Example 1 This embodiment provides an enzymatic hydrolysis preparation process for ginseng essence for improving immunity and relieving fatigue, and the enzymatic hydrolysis preparation process comprises the following steps: (1) 100 g of fresh ginseng was washed, then steamed at 105°C for 5 min, and then squeezed twice, and then 1200 g of water was added to crush and pulp to obtain ginseng pulp; (2) sterilizing the ginseng slurry at 95° C. for 8 minutes, and then subjecting the ginseng slurry to ultrasonic treatment at room temperature, a frequency of 18 kHz, and 800 W for 30 minutes to obtain a sterilized slurry; (3) adding 18 g of complex enzyme (comprising 2.5 g of cellulase, 2.5 g of hemicellulase, 6.25 g of amylase, 1.25 g of pectinase, 5 g of cellobiase, and 0.5 g of protease) to the sterilized slurry, stirring the mixture at 55° C. for 16 h to obtain an enzymatic slurry; (4) inactivating the enzyme in the enzymatic hydrolysis slurry at 85° C. for 15 minutes, and then subjecting the slurry to three cycles of leaching and reflux at 80° C., each leaching and reflux time being 20 minutes, to obtain an extract; (5) The extract is low-temperature precipitated at 2°C for 8 hours, and then the supernatant is taken and filtered through five levels of fine filtration, and finally sterilized at 105°C for 50 minutes to obtain the ginseng essence for improving immunity and relieving fatigue. (After filling, each bottle has a specification of 30 mL, ginsenoside ≥ 81 mg, and 1 bottle is equivalent to 8 to 10 bowls of ginseng soup, and the absorption is also higher than that of directly eating ginseng).

[0034] Example 2 This embodiment provides an enzymatic hydrolysis preparation process for ginseng essence for improving immunity and relieving fatigue, and the enzymatic hydrolysis preparation process comprises the following steps: (1) 100 g of fresh ginseng was washed, then steamed at 110°C for 3 min, and then squeezed twice, and then 2000 g of water was added to crush and pulp to obtain ginseng pulp; (2) sterilizing the ginseng slurry at 98° C. for 5 minutes, and then subjecting the ginseng slurry to ultrasonic treatment at room temperature, a frequency of 20 kHz, and 500 W for 35 minutes to obtain a sterilized slurry; (3) adding 22 g of complex enzyme (comprising 3.14 g of cellulase, 3.14 g of hemicellulase, 6.28 g of amylase, 2.09 g of pectinase, 6.28 g of cellobiase, and 1.07 g of protease) to the sterilized slurry, stirring the mixture at 55° C. for 18 h to obtain an enzymatic slurry; (4) inactivating the enzyme in the enzymatic hydrolysis slurry at 95° C. for 10 min, and then subjecting the slurry to 3 cycles of leaching and reflux at 85° C., each leaching and reflux time being 30 min, to obtain an extract; (5) The extract is low-temperature precipitated at 1°C for 7 hours, and then the supernatant is taken and filtered through 5 levels of fine filtration, and finally sterilized at 110°C for 40 minutes to obtain the ginseng essence for improving immunity and relieving fatigue. (After filling, each bottle has a specification of 30 mL, ginsenoside ≥ 81 mg, 1 bottle is equivalent to 8 to 10 bowls of ginseng soup, and the absorption is also higher than that of directly eating ginseng).

[0035] Example 3 This embodiment provides an enzymatic hydrolysis preparation process for ginseng essence for improving immunity and relieving fatigue, and the enzymatic hydrolysis preparation process comprises the following steps: (1) 100 g of fresh ginseng was first washed, then steamed at 108°C for 4 min, and then finely squeezed twice, and then 1600 g of water was added to crush and pulp the ginseng to obtain ginseng pulp; (2) sterilizing the ginseng slurry at 96° C. for 5 minutes, and then subjecting the ginseng slurry to ultrasonic treatment at room temperature, a frequency of 19 kHz, and 650 W for 32 minutes to obtain a sterilized slurry; (3) adding 20 g of complex enzyme (comprising 2.82 g of cellulase, 2.82 g of hemicellulase, 6.21 g of amylase, 1.69 g of pectinase, 5.64 g of cellobiase, and 0.82 g of protease) to the sterilized slurry, stirring the mixture at 55° C. for 17 h to obtain an enzymatic slurry; (4) inactivating the enzyme in the enzymatic hydrolysis slurry at 90° C. for 12 minutes, and then subjecting the slurry to three cycles of leaching and reflux at 82° C., each leaching and reflux time being 25 minutes, to obtain an extract; (5) The extract is low-temperature precipitated at 1°C for 7 hours, and then the supernatant is taken and filtered through 5 levels of fine filtration, and finally sterilized at 108°C for 45 minutes to obtain the ginseng essence for improving immunity and relieving fatigue. (After filling, each bottle has a specification of 30 mL, ginsenoside ≥ 81 mg, 1 bottle is equivalent to 8 to 10 bowls of ginseng soup, and the absorption is also higher than that of directly eating ginseng).

[0036] Test example The ginseng essence obtained in Example 3 was tested, and the contents of its main components are shown in Table 1.

[0037] Table 1

[0038] Animal experiment test examples 1. Anti-fatigue efficacy test 1. Test materials 1.1 Sample preparation information Ginseng essence, directly absorb the original liquid (in the anti-fatigue effect, the ginseng essence is prepared by referring to the process of Example 3, and the concentration is gradually increased).

[0039] Positive control: Salidroside, white powder, batch number C23HY06052605B, Huazhong Haiwei (Beijing) Gene Technology Co., Ltd., solvent is standard dilution water.

[0040] 1.2 Animal experiments Zebrafish were kept in aquaculture water at 28°C (water quality: 200 mg instant sea salt was added to 1 L of reverse osmosis water, conductivity was 450-550 μS / cm; pH was 6.5-8.5; hardness was 50-100 mg / L CaCO 3 ), bred and provided by the fish farming center of the testing company, the experimental animal use license number is: SYXK (Zhejiang) 2022-0004, the breeding and management complies with the requirements of the international AAALAC certification (certification number: 001458), IACUC ethics review number: IACUC-2024-10699-01.

[0041] 1.3 Instruments, consumables and reagents Dissecting microscope (SZX7, OLYMPUS, Japan); CCD camera (VertA1, Shanghai Tusen Vision Technology Co., Ltd., China); precision electronic balance (CP214, OHAUS, USA); 6-well plate (Zhejiang Bellambo Biotechnology Co., Ltd., China); 96-well plate (Nest Biotech, China); behavior analyzer (Zebra Lab 3.22.3.31, Viewpoint, France).

[0042] Anhydrous sodium sulfite (Batch No. I1428128, Shanghai Aladdin Biochemical Technology Co., Ltd., China).

[0043] 2. Detection Method 2.1 Maximum detectable concentration (MTC) determination Wild-type AB zebrafish at 4 days post-fertilization (dpf) were randomly selected in a 6-well plate, and 30 zebrafish were treated in each well (experimental group). Samples were given in water (concentrations as shown in Table 2), and a normal control group and a model control group were set up at the same time, with a volume of 3 mL per well. After 1 day of treatment at 28°C, all experimental groups except the normal control group were given anhydrous sodium sulfite in water to establish a zebrafish fatigue model. After continuing to treat at 28°C for 1 hour, the MTC of the samples on the model zebrafish was measured.

[0044] 2.2 Evaluation of anti-fatigue efficacy 4dpf wild-type AB strain zebrafish were randomly selected in a 6-well plate, and 30 zebrafish were treated in each well (experimental group). The samples were given water-soluble (concentrations are shown in Table 3), the positive control salidroside was 4000μg / mL, and a normal control group and a model control group were set up at the same time, with a capacity of 3mL per well. After 1 day of treatment at 28℃, except for the normal control group, all other experimental groups were given anhydrous sodium sulfite in water to establish a zebrafish fatigue model. After 1h of treatment at 28℃, 10 zebrafish were randomly selected from each experimental group and placed in a 96-well plate. The total movement distance (A) of the zebrafish was measured using a behavior analyzer, and the statistical analysis results of this indicator were used to evaluate the anti-fatigue efficacy of the sample. The statistical treatment results are expressed as mean±SE. The anti-fatigue efficacy calculation formula is as follows: Anti-fatigue effect (%) = A (sample group) - A (model control group) / A (model control group) * 100% SPSS 26.0 software was used for statistical analysis, and p < 0.05 indicated that the difference was statistically significant.

[0045] 3. Test results 3.1 MTC Under the present testing conditions, the MTC of ginseng essence in combating fatigue is 62.5 μL / mL, as shown in Table 2.

[0046] Table 2 Results of the experimental study on the concentration of anti-fatigue effect of samples (n=30)

[0047] 3.2 Evaluation of anti-fatigue efficacy Under the test conditions, ginseng essence has anti-fatigue effect, which is specifically manifested in increasing the total exercise distance, and the total exercise distance is increased by 67% compared with the model control group. See Table 3 for details. Figure 1 , Figure 2 and Figure 3 .

[0048] Table 3 Test results of sample anti-fatigue efficacy evaluation (n=10)

[0049] Compared with the model control group, ***p<0.001.

[0050] (II) Immunity enhancement test 1. Sample preparation information Ginseng essence, directly absorb the original liquid (in the anti-fatigue effect, the ginseng essence is prepared by referring to the process of Example 3, and the concentration is gradually increased).

[0051] Positive control: Bailing capsules, batch number 2307029D, Hangzhou Sino-US Huadong Pharmaceutical Co., Ltd., stored in a cool and dry place. The solvent is standard dilution water.

[0052] 1.2 Animal experiments Zebrafish were kept in aquaculture water at 28°C (water quality: 200 mg instant sea salt was added to 1 L of reverse osmosis water, conductivity was 450-550 μS / cm; pH was 6.5-8.5; hardness was 50-100 mg / L CaCO 3 ), bred and provided by the fish farming center of the testing company, the experimental animal use license number is: SYXK (Zhejiang) 2022-0004, the breeding and management complies with the requirements of the international AAALAC certification (certification number: 001458), IACUC ethics review number: IACUC-2024-11002-01.

[0053] 1.3 Instruments, consumables and reagents Dissecting microscope (SZX7, OLYMPUS, Japan); CCD camera (VertA1, Shanghai Tusen Vision Technology Co., Ltd., China); precision electronic balance (CP214, OHAUS, USA); electric focus continuous zoom fluorescence microscope (AZ100, Nikon, Japan); microinjection instrument (IM-300, Narishige, Japan); needle puller (PC-10, Narishige, Japan); 6-well plate (Zhejiang Bellanbo Biotechnology Co., Ltd., China).

[0054] Vinorelbine tartrate injection (batch number 190401, Jiangsu Hausen Pharmaceutical Co., Ltd., China); 0.9% sodium chloride injection (batch number S21091609, Hunan Kelun Pharmaceutical Co., Ltd., China); methylcellulose (batch number C2004046, Shanghai Aladdin Biochemical Technology Co., Ltd., China).

[0055] 2. Detection Method 2.1 Maximum detectable concentration (MTC) determination Transgenic macrophage green fluorescent zebrafish at 3 days post fertilization (3dpf) were randomly selected in 6-well plates, and 30 zebrafish were treated in each well (experimental group). Water-soluble samples (concentrations are shown in Table 4) were administered, and normal control group and model control group were set up at the same time, with a capacity of 3mL per well. Except for the normal control group, all other experimental groups were intravenously injected with vinorelbine tartrate injection to establish a zebrafish immunodeficiency model. After 2 days of treatment at 28℃, the MTC of the samples on the model zebrafish was measured.

[0056] 2.2 Evaluation of immune enhancement efficacy-1 3dpf transgenic macrophage green fluorescent zebrafish were randomly selected in a 6-well plate, and 30 zebrafish were treated in each well. The water-soluble samples (concentrations are shown in Table 5), the positive control Bailing capsule concentration was 15.0μg / mL, and a normal control group and a model control group were set up at the same time, with a capacity of 3mL per well. Except for the normal control group, the other experimental groups were intravenously injected with vinorelbine tartrate injection to induce an immunodeficiency model. After 2 days of treatment at 28℃, 10 zebrafish were randomly selected from each experimental group and photographed under a fluorescence microscope. The data were analyzed and collected using NIS-Elements D 3.20 advanced image processing software, and the fluorescence intensity of macrophages in the tail vein of zebrafish (C) was analyzed. The statistical analysis results of this indicator were used to evaluate the sample's immune-enhancing efficacy. The statistical processing results are expressed as mean±SE. The formula for calculating the immune-enhancing efficacy is as follows: Immunity enhancement effect (%) = C (sample group) - C (model control group) / C (model control group) * 100% SPSS26.0 software was used for statistical analysis, and p<0.05 indicated that the difference was statistically significant.

[0057] 2.3 Evaluation of immune enhancement efficacy-2 3 dpf transgenic macrophage green fluorescent zebrafish were randomly selected in a 6-well plate, and 30 zebrafish were treated in each well. The water-soluble samples (concentrations are shown in Table 6), the positive control Bailing capsule concentration was 15.0 μg / mL, and the normal control group and model control group were set up at the same time, with a capacity of 3 mL per well. Except for the normal control group, the other experimental groups were intravenously injected with vinorelbine tartrate injection to induce an immunodeficiency model. After 3 days of treatment at 28°C, 10 zebrafish were randomly selected from each experimental group and photographed under a fluorescence microscope. The data were analyzed and collected using NIS-Elements D 3.20 advanced image processing software, and the fluorescence intensity (D) of macrophages in the tail vein of zebrafish was analyzed. The statistical analysis results of this indicator were used to evaluate the sample's immune-enhancing efficacy. The statistical processing results are expressed as mean ± SE. The formula for calculating the immune-enhancing efficacy is as follows: Immunity enhancement effect (%) = D (sample group) - D (model control group) / D (model control group) * 100% SPSS26.0 software was used for statistical analysis, and p<0.05 indicated that the difference was statistically significant.

[0058] 3. Detection methods 3.1 MTC Under the test conditions, the MTC of ginseng essence on zebrafish for enhancing immunity was 7.81 μL / mL, as shown in Table 4.

[0059] Table 4 Experimental results of the maximum detection concentration of samples for enhancing immune efficacy (n=30)

[0060] 3.2 Evaluation of immune enhancement efficacy-1 Under the experimental conditions, ginseng essence has the effect of enhancing immunity, which is specifically manifested by increasing the fluorescence intensity of tail vein macrophages, and the fluorescence intensity of tail vein macrophages increased by 22% compared with the model control group. See Table 5 for details. Figure 4 , Figure 5 and Figure 6 .

[0061] Table 5 Results of the sample immune enhancement efficacy evaluation experiment-1 (n=10)

[0062] Compared with the model control group, ***p<0.001.

[0063] 3.3 Evaluation of immune enhancement efficacy-2 Under the experimental conditions, ginseng essence has the effect of enhancing immunity, which is specifically manifested by increasing the fluorescence intensity of tail vein macrophages, and the fluorescence intensity of tail vein macrophages increased by 29% compared with the model control group. See Table 6 for details. Figure 7 , Figure 8 and Fig. 9 .

[0064] Table 6 Samples' immune enhancement efficacy evaluation test results-2 (n=10)

[0065] Compared with the model control group, ***p<0.001.

[0066] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An enzymatic preparation process for ginseng essence for improving immunity and relieving fatigue, characterized in that: The enzymatic preparation process comprises the following steps: (1) washing, steam-scalding, fine-pressing and pulping fresh ginseng in sequence to obtain ginseng pulp; (2) sterilizing and ultrasonically treating the ginseng slurry in sequence to obtain a sterilized slurry; (3) adding a composite enzyme to the sterilization slurry and stirring the mixture to obtain an enzymatic slurry; (4) sequentially performing enzyme inactivation, cyclic leaching and reflux on the enzymatic hydrolysis slurry to obtain an extract; (5) The extract is allowed to settle at low temperature, and the supernatant is taken out, and then filtered and sterilized to obtain the ginseng essence for improving immunity and relieving fatigue.

2. The enzymatic hydrolysis preparation process of ginseng essence according to claim 1, characterized in that: In step (1), the steaming temperature is 105-110°C, and the steaming time is 3-5 minutes.

3. The enzymatic hydrolysis preparation process of ginseng essence according to claim 1, characterized in that: In step (1), water is added for pulping at a material-to-water ratio of 1:12 to 20.

4. The enzymatic hydrolysis preparation process of ginseng essence according to claim 1, characterized in that: In step (2), the sterilization temperature is 95-98°C and the sterilization time is 5-8 minutes.

5. The enzymatic hydrolysis preparation process of ginseng essence according to claim 1, characterized in that: In step (2), the frequency of the ultrasonic treatment is 18-20 kHz, the treatment power is 500-800 W, and the treatment time is 30-35 min.

6. The enzymatic hydrolysis preparation process of ginseng essence according to claim 1, characterized in that: In step (3), the complex enzyme comprises the following raw materials in parts by weight: 10-15 parts of cellulase, 10-15 parts of hemicellulase, 25-30 parts of amylase, 5-10 parts of pectinase, 20-30 parts of cellobiase, and 2-5 parts of protease; And / or, in step (3), the stirring treatment time is 16-18 hours, and the stirring treatment temperature is 55°C.

7. The enzymatic hydrolysis preparation process of ginseng essence according to claim 1, characterized in that: In step (4), the enzyme inactivation temperature is 85-95°C and the enzyme inactivation time is 10-15 minutes; And / or, in step (4), the number of cycles of leaching and reflux is 3 to 4 times, the leaching and reflux temperature is 80 to 85° C., and the time of each leaching and reflux is 20 to 30 minutes.

8. The enzymatic hydrolysis preparation process of ginseng essence according to claim 1, characterized in that: In step (5), the temperature of the low-temperature precipitation is 0-2°C, and the time of the low-temperature precipitation is 7-8h; And / or, in step (5), the sterilization temperature is 105-110° C., and the sterilization time is 40-50 min.

9. The ginseng essence for improving immunity and relieving fatigue obtained by the enzymatic preparation process according to any one of claims 1 to 8.

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