Biopesticide for preventing and treating mango anthracnose

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a biopesticide for preventing and treating mango anthracnose. The invention relates to a biopesticide. Active ingredients of the biopesticide are formed by compounding cytosinpeptidamycin and eugenol or L-carvone. According to the biopesticide disclosed by the invention, the compound combination of cytosinpeptidamycin and eugenol or L-carvone is taken as an active ingredient, and a hypha growth rate method is adopted to find that the compound combination can generate a synergistic effect in a verified compounding proportion, so that the control effect on mango anthracnose can be improved, and meanwhile, the use dosage of the pesticide can be reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a biological pesticide for preventing and controlling mango anthracnose. Background Art

[0002] Anthracnose is one of the common diseases of tropical fruits such as mango, banana and avocado. It is caused by infection with the genus Colletotrichum. There is an incubation period. The disease will occur as the fruit matures, producing dark brown or black lesions on the surface of the fruit, causing the host to rot rapidly. According to surveys, Colletotrichum gloeosporioide is the main pathogen causing mango anthracnose in southern my country. As the largest population in the genus Colletotrichum, the typical symptom of Colletotrichum gloeosporioide infecting mango fruit is the formation of sunken, protruding, dark brown to black rotten spots, which are usually limited to the peel and can invade the flesh in severe cases. At the same time, in the late stage of the lesion, the pathogen will stack and aggregate, and a large number of orange or orange-red conidia clumps visible to the naked eye will appear on the lesion site.

[0003] Chemical fungicides are one of the common means of preventing and controlling mango anthracnose, but the commonly used control agents such as carbendazim are old agents with a long history and high drug resistance, and the control effect is not ideal. Therefore, the demand for green and efficient agents for the prevention and control of mango anthracnose is of great significance to mango production. In the prior art, the existing agents can be combined to prevent and control the disease, but the effects of different compound combinations are unpredictable, which may produce synergistic effects or antagonistic effects, and the control effects of different compound combinations on different diseases are also unpredictable.

[0004] So far, there is no report on the use of pyrimidine antibiotics in combination with eugenol or L-carvone for the prevention and treatment of mango anthracnose.

[0005] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0006] The object of the present invention is to provide a biological pesticide for preventing and controlling mango anthracnose, so as to solve the problem that chemical fungicides are one of the common means for preventing and controlling mango anthracnose, but the commonly used control agents such as carbendazim are old agents with a long time and high drug resistance, and the control effect is not ideal.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A biological pesticide, the active ingredient of which is a compound of pyrimidine mycin and eugenol or L-carvone.

[0009] Furthermore, the mass ratio of pyrimidinemycin to eugenol is 1-20:5-1.

[0010] Furthermore, the mass ratio of pyrimidinemycin to L-carvone is 1-10:10-1.

[0011] Furthermore, the mass of the active ingredient accounts for 1-30% of the total mass of the biological pesticide, and the remainder is auxiliary ingredients allowed to be added in pesticide science.

[0012] Furthermore, the mass of the active ingredient accounts for 5-20% of the total mass of the biopesticide.

[0013] The invention also provides application of the biological pesticide in preventing and controlling mango anthracnose.

[0014] Furthermore, the mango anthracnose is caused by infection by Colletotrichum gloeosporioides.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] (1) The biological pesticide of the present invention uses a compound combination of pyrimidine mycin and eugenol or L-carvone as an active ingredient. The mycelium growth rate method is used to find that the compound combination can produce a synergistic effect within the verified compound ratio, thereby improving the control effect on mango anthracnose and reducing the dosage of the agent.

[0017] (2) In the biopesticide of the present invention, pyrimidine mycin, eugenol and L-carvone are all active ingredients of the biopesticide, which shows that the biopesticide of the present invention is green and environmentally friendly.

[0018] (3) The biopesticide of the present invention is compounded with two bactericidal components. Compared with a single agent, it can reduce the risk of drug resistance and delay the generation and development of drug resistance in pathogens. DETAILED DESCRIPTION

[0019] The following is a clear and complete description of the technical solution of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without creative work are within the scope of protection of the present invention.

[0020] 1. Materials and Methods

[0021] 1.1 Test strains

[0022] Colletotrichum gloeosporioides, isolated from infected mango fruits.

[0023] 1.2 Test agents

[0024] 30% pyrimidine mycin stock drug (Dalian Guanfa Pharmaceutical Co., Ltd.), 98.5% eugenol (commercially available), 99% L-carvone (commercially available).

[0025] Pyrimidinemycin is directly diluted with a 0.1% Tween-80 aqueous solution to form a single-dose stock solution; eugenol and L-carvone are first dissolved with dimethyl sulfoxide and then diluted with a 0.1% Tween-80 aqueous solution to form a single-dose stock solution; multiple groups of proportions are set up, and each single-dose stock solution and proportioned mixture are diluted with a 0.1% Tween-80 aqueous solution into 5 mass concentration gradients according to an equal ratio method, and the above medicines are prepared and used immediately.

[0026] 1.3 Test methods

[0027] The mycelium growth rate method was used to determine the toxicity of single agents and mixed agents to the test strain Colletotrichum gloeosporioides. Specifically, the PDA medium cooled to about 50°C and the drug solution were mixed evenly in a volume ratio of 9:1, poured into a culture dish with a diameter of 9 cm, 10 mL per dish, and cooled to form a drug-containing plate. At the same time, the PDA medium cooled to about 50°C and the 0.1% Tween-80 aqueous solution were mixed evenly in a volume ratio of 9:1, poured into a culture dish with a diameter of 9 cm, 10 mL per dish, and cooled to form a plate as a blank control. Each treatment was repeated 4 times, and each repeat had 2 plates.

[0028] Use a sterile inoculation loop to inoculate the test strain onto the PDA medium, and culture it in a constant temperature incubator at 28°C in the dark for propagation. After 6 days, use a hole puncher to punch a 5mm diameter bacterial cake along the edge of the colony with the same radius. Inoculate the bacterial cake into the center of the drug-containing plate and the blank control plate, and inoculate one bacterial cake per plate. Cover the plate and culture it in a constant temperature incubator at 8°C. When the colony diameter of the blank control grows to about 6cm, use the cross method to measure the colony diameter of each treatment, and calculate the inhibition rate of mycelial growth by different treatments.

[0029] Inhibition rate (%) = (control colony diameter - control colony diameter) - (-treated colony diameter - colony diameter) × 100

[0030] 1.4 Data Analysis

[0031] DPS software was used for data statistical analysis. The logarithmic value of fungicide concentration was taken as x and the corresponding probability value of mycelium growth inhibition rate was taken as y for regression analysis to calculate the toxicity EC of the fungicide to the formula strain. 50 The co-toxicity coefficient (CTC) was calculated using the Sun Yunpei method.

[0032] 1.5 Measurement results

[0033] The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC). CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Table 1-2.

[0034] Table 1 Indoor bioactivity test of pyrimidine antibiotics and eugenol against Colletotrichum gloeosporioides

[0035] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Pyrimidine 62.9532 100.0000 - -- Eugenol 13.7221 458.7723 -- -- Pyrimidine 1: eugenol 5 11.8457 531.4435 398.9770 133.2015 Pyrimidine 1: eugenol 3 13.2302 475.8295 369.0793 128.9234 Pyrimidine 1: Eugenol 1 18.1936 346.0184 279.3862 123.8495 Pyrimidine 3: eugenol 1 21.4573 293.3883 189.6931 154.6647 Pyrimidine 5: eugenol 1 32.5479 193.4171 159.7954 121.0405 Pyrimidine 10: eugenol 1 34.1257 184.4745 132.6157 139.1046 Pyrimidine 20: eugenol 1 42.9642 146.5248 117.0844 125.1446

[0036] As shown in Table 1, when the mass ratio of pyrimidine antibiotics and eugenol is in the range of 1-20:5-1, the co-toxicity coefficient against the pathogen of mango anthracnose is greater than 120, indicating that the combination of the two within this range shows a synergistic effect and can improve the control effect of mango anthracnose.

[0037] Table 2 Indoor bioactivity determination of pyrimidine antibiotics and L-carvone against Colletotrichum gloeosporioides of mango

[0038]

[0039]

[0040] As shown in Table 2, when the mass ratio of pyrimidine antibiotics and L-carvone is in the range of 1-10:10-1, the co-toxicity coefficient against mango anthracnose Colletotrichum gloeosporioides is greater than 120, indicating that the combination of the two within this range shows a synergistic effect and can improve the control effect of mango anthracnose.

[0041] In summary, the biological pesticide of the present invention uses a compound combination of pyrimidine mycin and eugenol or L-carvone as an active ingredient. The mycelium growth rate method is used to find that the compound combination can produce a synergistic effect within the verified compound ratio, which can improve the control effect on mango anthracnose and reduce the dosage of the agent.

Claims

1. A biological pesticide, characterized in that: Its active ingredient is a combination of pyrimidine mycin and eugenol or L-carvone.

2. The biopesticide according to claim 1, characterized in that: The mass ratio of pyrimidinemycin to eugenol is 1-20:5-1.

3. The biopesticide according to claim 1, characterized in that: The mass ratio of pyrimidinemycin to L-carvone is 1-10:10-1.

4. The biopesticide according to claim 1, characterized in that: The mass of the active ingredient accounts for 1-30% of the total mass of the biological pesticide, and the remainder is auxiliary ingredients allowed to be added in pesticide science.

5. The biopesticide according to claim 4, characterized in that: The mass of the active ingredient accounts for 5-20% of the total mass of the biopesticide.

6. Use of the biological pesticide according to any one of claims 1 to 5 in preventing and controlling mango anthracnose.

7. The use according to claim 6, characterized in that: The mango anthracnose is caused by infection by Colletotrichum gloeosporioides.

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