A composition containing arbuscular mycorrhizae and its application in controlling peanut pests

Abstract

The present invention discloses a composition containing arbuscular mycorrhiza and its application in controlling peanut pests. The active ingredients of the composition are luteolin and arbuscular mycorrhizal fungi. The present invention provides a composition containing arbuscular mycorrhiza, comprising luteolin and arbuscular mycorrhizal fungi. Luteolin is a natural flavonoid compound with various biological activities such as insect resistance, feeding inhibition, and antioxidant properties. By directly affecting the digestive system, behavior, and development of pests, luteolin can effectively reduce the damage caused by pests. Arbuscular mycorrhizal fungi are a class of beneficial microorganisms that symbiotically associate with plant roots. When the two are compounded, the presence of arbuscular mycorrhizal fungi can help peanuts better absorb nutrients, thereby improving the plant's own stress resistance and making peanuts more resistant when attacked by pests. Luteolin directly inhibits pests, and the combination of the two can effectively improve the persistence of the insecticidal effect and reduce the sensitivity to the repeated occurrence of pests.

Description

Technical Field

[0001] The present invention relates to the technical field of pest control, and particularly relates to a composition containing arbuscular mycorrhiza and its application in controlling peanut pests. Background Art

[0002] Peanut, originally named Arachis hypogaea Linn., belongs to the Rosales family Leguminosae and is an important economic and oil crop. China's peanut export trade occupies an important position in the world peanut trade. During the peanut production process, it is extremely vulnerable to the stress and damage of various pests, resulting in a significant decline in peanut yield and quality. White grubs are the general term for the larvae of the superfamily Scarabaeidae in the Coleoptera order. They are the group with the most species, the widest distribution, and the most serious damage among underground pests. Their number accounts for more than 80% of the number of underground pests, affecting peanut yield and quality. Aphids are pests with a piercing-sucking mouthpart that damage the above-ground parts of plants. Aphids pierce peanut leaves, tender stems, tender buds, and pods with their mouthparts and suck the plant sap. After the plant is sucked, the leaves will curl, turn yellow, and even fall off prematurely, and the growth will stagnate.

[0003] Currently, chemical agents are still mainly used to control white grubs and aphids. The excessive and frequent use of chemical pesticides not only increases the drug resistance of white grubs but also causes irreversible damage to the ecological environment. Biological control has the advantages of good safety, no environmental pollution, and long-lasting effects. In recent years, it has achieved rapid development, its research has gradually deepened, and more and more biological control methods have been promoted and applied. Arbuscular mycorrhizal fungi (AMF) belong to the Glomeromycota phylum and are a type of endophytic fungi. They are soil-borne fungi widely present in agroforestry composite ecosystems and can form mutualistic symbiosis with the vast majority of plants. Luteolin is a naturally occurring plant flavonoid compound that is widely present in a variety of plants, especially some vegetables, herbs, and fruits. Compared with conventional chemical pesticides, luteolin is a natural flavonoid compound with lower toxicity to humans and the environment. Therefore, it is considered an environmentally friendly insecticide with less impact on non-target organisms (such as beneficial insects like bees) and the human body. Since the action mechanism of luteolin is different from that of conventional pesticides, using it can effectively reduce the problem of pest drug resistance caused by the long-term use of conventional pesticides. As an alternative or supplementary natural insecticide, it has good application prospects. Currently, there is no report on the compound application of luteolin and arbuscular mycorrhizal fungi in controlling peanut pests. Summary of the Invention

[0004] Aiming at the above-mentioned existing technology, the purpose of the present invention is to provide a composition containing arbuscular mycorrhiza and its application in controlling peanut pests.

[0005] To achieve the above object, the present invention adopts the following technical solutions:

[0006] In a first aspect of the present invention, there is provided a composition containing arbuscular mycorrhiza, wherein the active ingredients of the composition are luteolin and arbuscular mycorrhizal fungi.

[0007] Preferably, in the composition, the concentration of luteolin is 0.01 - 0.15 mg / L; the viable count of arbuscular mycorrhizal fungi ≥ 1000 spores / mL.

[0008] In a second aspect of the present invention, there is provided the use of the above composition in controlling peanut pests.

[0009] Preferably, the peanut pests are white grubs or aphids.

[0010] Preferably, the arbuscular mycorrhizal fungi is Rhizophagus irregularis.

[0011] Preferably, the preservation number of the Rhizophagus irregularis is CCTCC NO: M2014532.

[0012] In a third aspect of the present invention, there is provided a method for controlling peanut pests, wherein the above composition is applied to the peanuts to be treated by means of root absorption, spraying or smearing.

[0013] Advantages of the present invention:

[0014] 1. The present invention provides a composition containing arbuscular mycorrhiza, comprising luteolin and arbuscular mycorrhizal fungi. Luteolin is a natural flavonoid compound with various biological activities such as insect resistance, feeding inhibition and antioxidant properties. By directly affecting the digestive system, behavior and development of pests, luteolin can effectively reduce the damage of pests; Arbuscular mycorrhizal fungi are a class of beneficial microorganisms symbiotic with plant roots, which can promote the root growth of peanuts, improve the ability of peanuts to absorb water and nutrients, thereby enhancing the resistance and overall health of plants; By compounding the two, the presence of arbuscular mycorrhizal fungi can help peanuts better absorb nutrients, thereby improving the plant's own stress resistance and making peanuts more resistant when attacked by pests, while luteolin directly inhibits pests, and the combination of the two can effectively improve the persistence of the insecticidal effect and reduce the sensitivity to the repeated occurrence of pests.

[0015] 2. The main components of the composition containing arbuscular mycorrhiza proposed by the present invention are natural compounds (luteolin) and beneficial microorganisms (arbuscular mycorrhizal fungi), with low toxicity and relatively friendly to the environment. Compared with chemical pesticides, this composition can reduce the environmental pollution caused by chemical agents and reduce the harm to non-target organisms. Detailed implementation manners

[0016] It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which this application belongs.

[0017] In order to enable those skilled in the art to more clearly understand the technical solution of the present application, the technical solution of the present application will be described in detail below in combination with specific embodiments.

[0018] The test materials used in the embodiments of the present invention are all conventional test materials in the art and can be obtained through commercial channels.

[0019] The preservation number of the arbuscular mycorrhizal fungus (AMF) Rhizophagus irregularis CD-1 used in the present invention is CCTCC NO: M2014532, which is recorded in Patent CN104450536B, and the AMF inoculant is prepared with reference to the method in Example 2 of Patent CN104450536B.

[0020] Example 1: Preparation of a composition containing arbuscular mycorrhiza:

[0021] The AMF inoculant, luteolin and deionized water are mixed evenly so that the concentration of luteolin is 0.05 mg / L; the viable count of arbuscular mycorrhizal fungi is 1000 spores / mL to obtain a composition.

[0022] Comparative Example 1:

[0023] A single AMF inoculant is selected, and the viable count of the inoculant is 1000 spores / mL.

[0024] Comparative Example 2:

[0025] A single luteolin solution is selected, in which the concentration of luteolin is 0.05 mg / L.

[0026] Test Example 1: Insecticidal effect of the composition containing arbuscular mycorrhiza on white grubs

[0027] The peanut variety is Huayu 22. After the peanut seeds are disinfected and germinated to show white buds, the seeds with the same germination situation are selected and planted in sterilized potting soil. The test is set up in 2 groups:

[0028] Test insect source:

[0029] The white grubs are collected from the peanut experimental field of the Plant Protection Experimental Station of Shandong Agricultural University, which is a mixture of Holotrichia parallela, Holotrichia oblita, and Anomala corpulenta in a ratio of 1:1:1.

[0030] A total of 4 treatment groups were set up in the experiment:

[0031] Treatment 1: Apply 10 mL of the composition containing arbuscular mycorrhiza prepared in Example 1 to each peanut plant;

[0032] Treatment 2: Apply 10 mL of the AMF agent of Comparative Example 1 to each peanut plant;

[0033] Treatment 3: Apply 10 mL of the luteolin solution of Comparative Example 2 to each peanut plant;

[0034] The control group applied 10 mL of clear water to each peanut plant. Subsequently, the entire soil was thoroughly watered, 2 seeds were sown in each pot, and the cultivation was carried out in a solar greenhouse. Each group of experiments was repeated three times.

[0035] The peanut rhizomes of different treatment groups were separately taken to feed the white grubs, and the fresh rhizomes were replaced every day. The growth and development of the white grubs were observed and recorded, and the growth status was recorded. 30 white grubs were selected in each group, and the mortality rate up to 10 d was counted.

[0036] Experimental results:

[0037] Table 1 Effects of different treatments on the mortality rate of white grubs

[0038] Treatment Mortality rate (%) Treatment 1 86.67±5.77a Treatment 2 13.33±5.09c Treatment 3 43.33±8.39b Control group 2.22±1.11d

[0039] It can be concluded from Table 1 that when the white grubs fed on the peanut rhizomes inoculated with the composition containing arbuscular mycorrhiza prepared in Example 1, it had an inhibitory effect on their growth and development, and the insecticidal effect was very significant, reaching more than 85. For Treatment 2 and Treatment 3, when the white grubs fed on the peanut rhizomes inoculated with the single AMF agent and luteolin solution respectively, the mortality rates were 12.54% and 43.21% respectively, and the sum of the mortality rates of the white grubs in the two treatments was 55.75%. This shows that the compounding of arbuscular mycorrhiza and luteolin in the present invention has a synergistic effect on improving the insecticidal effect against white grubs.

[0040] Example 2: Study on the resistance of the composition containing arbuscular mycorrhiza to white grubs in field peanuts:

[0041] The peanut variety was Huayu 22, and the field experiment site was a place where aphids and white grubs had been severely damaged in the Plant Protection Experimental Station of Shandong Agricultural University over the years.

[0042] Three peanut seeds were sown in each hole, and the experiment was divided into four treatments:

[0043] Treatment 1: Add 50 mL of the composition containing arbuscular mycorrhiza prepared in Example 1 to each hole;

[0044] Treatment 2: Add 50 mL of the AMF agent of Comparative Example 1 to each hole;

[0045] Treatment 3: Add 50 mL of the luteolin solution of Comparative Example 2 to each hole;

[0046] In the control group, 50 mL of clear water was added to each acupoint. It was covered with soil, and then the whole land was watered, and conventional field management was carried out.

[0047] Determination indexes:

[0048] 1. Investigation on the damage of white grubs:

[0049] Using the "Z"-type sampling method, 5 points were investigated in each plot, and 15 acupoints (1 m 2 ) were investigated at each point. The entire root system of the peanut plants and the rhizosphere soil around them were moved outside the acupoints, and the survival number of white grubs, the number of peanut pods, and the number of damaged pods in each acupoint and its rhizosphere soil were investigated and recorded respectively. The pod damage rate was calculated, and the results are shown in Table 2.

[0050] 2. Aphid population density:

[0051] During the aphid damage period, systematic field investigations were carried out, and the investigation was carried out about every 3 days. Using the random sampling method of 5 points in each plot, 5 m 2 of peanuts were investigated at each point. The number of aphids at each point was recorded respectively, and the average aphid population density was calculated. The results are shown in Table 3.

[0052] Table 2 Effects of different treatments on the damage of white grubs

[0053]

[0054] Table 3 Aphid population density of different treatments

[0055]

[0056]

[0057] As shown in Table 2 and Table 3, compared with the control group, the numbers of white grubs and aphids in Treatment 1 were significantly reduced, indicating that the composition containing arbuscular mycorrhiza of the present invention has good control effects on both white grubs and aphids.

[0058] The above are only the preferred embodiments of the present application and are not used to limit the present application. For those skilled in the art, the present application can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A composition containing arbuscular mycorrhiza, characterized in that, The active ingredients of the composition are luteolin and arbuscular mycorrhizal fungi; In the composition, the concentration of luteolin is 0.01 - 0.15 mg / L; the viable count of arbuscular mycorrhizal fungi ≥ 1000 spores / mL; The arbuscular mycorrhizal fungus is Rhizophagus irregularis, and the preservation number of Rhizophagus irregularis is CCTCC NO: M2014532.

2. Use of the composition according to claim 1 in controlling peanut pests; the peanut pests are white grubs.

3. A method for controlling peanut pests, characterized in that, The composition according to claim 1 is applied to the peanuts to be treated by root absorption, spraying or smearing; the peanut pests are white grubs.

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