Composition with antibacterial effect as well as preparation method and application thereof

Abstract

The invention discloses a composition with an antibacterial effect as well as a preparation method and application thereof. The composition comprises a porphyra haitanensis polyphenol extract, a dunaliella salina polyphenol extract and a coralline algae polyphenol extract. According to the composition disclosed by the invention, a more spectral and stable antibacterial effect can be realized, the defect of a single component on the antibacterial effect can be made up, the complementarity of each component is exerted, and the overall antibacterial activity is enhanced; and the stability of the obtained composition can be improved, and the composition can keep a relatively good antibacterial effect in the storage and use processes. Meanwhile, the composition has multiple effects of resisting bacteria, resisting inflammation, resisting oxidation and the like, and can be applied to the fields of food or cosmetic corrosion prevention, cosmetic acne removal, skin conditioning and the like, so that the composition has a good application prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemical industrial products, and in particular to a composition with antibacterial effect, a preparation method and application thereof. Background Art

[0002] As consumers' demand for natural, environmentally friendly and safe products grows, the cosmetics industry is gradually turning to natural ingredients, especially in the field of antimicrobial products. Traditional antimicrobial technology mainly relies on synthetic chemical ingredients, such as preservatives such as parabens and phenoxyethanol, which can effectively inhibit the growth of microorganisms and extend the shelf life of products. However, with the increasing concern about the safety of chemical ingredients and the demand for "green" products, natural antimicrobial agents have gradually become the mainstream choice in the market. This trend has led to the focus of research on natural antimicrobial ingredients such as plant extracts and essential oils, especially seaweed extracts, which not only have low toxicity, but also can effectively improve the biocompatibility of products, becoming a promising natural ingredient in the cosmetics industry.

[0003] Seaweed extracts are rich in a variety of active ingredients, such as polyphenols, carotenoids, minerals and vitamins, which give seaweed extracts a variety of biological activities. As a major active ingredient, seaweed polyphenols have become an important raw material for cosmetic research and development due to their excellent physical and chemical properties. In recent years, with the advancement of extraction technology, the application of advanced technologies such as supercritical fluid extraction and enzymatic extraction has greatly improved the purity of seaweed extracts and the yield of active ingredients, further broadening its application prospects in the field of cosmetics.

[0004] However, existing natural antimicrobial ingredients still face some challenges. The antimicrobial effect of a single raw material is usually limited, and the antibacterial spectrum is narrow, especially when dealing with multiple pathogenic microorganisms, the effect may not be ideal. In addition, many natural ingredients lose their antimicrobial activity during storage and use, showing instability. Therefore, researchers are seeking more effective natural antimicrobial ingredients to meet the market demand for efficient, safe, and broad-spectrum natural antimicrobial agents.

[0005] In view of this, the present invention is proposed. Summary of the invention

[0006] The object of the present invention is to provide a composition with antibacterial efficacy and a preparation method and application thereof, through which a broader spectrum and more stable antibacterial effect can be achieved, and the stability under different environments can be improved to overcome the above-mentioned defects.

[0007] The present invention is achieved in that: In a first aspect, the present invention provides a composition with antibacterial efficacy, comprising a polyphenol extract of Porphyra haitanensis, a polyphenol extract of Dunaliella salina and a polyphenol extract of Coralline; The mass ratio of the polyphenol extract of Porphyra haitanensis, the polyphenol extract of Dunaliella salina and the polyphenol extract of Coralline algae in the above composition is (1-3): (1-3): (1-2).

[0008] In a second aspect, the present invention provides a method for preparing the above-mentioned composition, which comprises: extracting the raw materials with ethanol for the first time, ultrasonicating, concentrating and purifying them to obtain polyphenol extracts from Porphyra haitanensis, polyphenol extracts from Dunaliella salina and polyphenol extracts from coralline algae, and then mixing them according to proportion.

[0009] In a third aspect, the present invention provides use of the above composition in the preparation of a preservative.

[0010] In a fourth aspect, the present invention provides use of the above composition in the preparation of an antibacterial agent.

[0011] In a fifth aspect, the present invention provides use of the above composition in the preparation of an anti-acne product.

[0012] In a sixth aspect, the present invention provides use of the above composition in preparing a skin conditioning product.

[0013] The present invention has the following beneficial effects: (1) The present invention combines the polyphenol extract of Porphyra haitanensis, the polyphenol extract of Dunaliella salina and the polyphenol extract of Coralline algae to obtain a composition that achieves a more stable antibacterial effect, can make up for the deficiency of a single component in the antibacterial effect, give play to the complementarity of each component, and enhance the overall antibacterial activity.

[0014] (2) The present invention improves the stability of the obtained composition by compounding the three seaweed polyphenol extracts, so that it can maintain a good antibacterial effect during storage and use.

[0015] (3) The composition of the present invention has multiple functions such as antibacterial, anti-inflammatory and antioxidant, and can be applied to the fields of food or cosmetic preservation, and cosmetic acne removal. Therefore, the composition of the present invention has good application prospects. DETAILED DESCRIPTION

[0016] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0017] Seaweed polyphenols are extracted from seaweeds such as brown algae, red algae and green algae. These polyphenol compounds not only have significant antioxidant functions, can effectively remove free radicals and slow down the aging process, but also can inhibit the growth of a variety of microorganisms. The antibacterial mechanism of seaweed polyphenols is mainly: it can penetrate the cell membrane of microorganisms and destroy its structure, thereby inhibiting the growth and reproduction of microorganisms; by inhibiting the activity of key enzymes required for microbial metabolism, it further prevents the metabolism and growth of microorganisms; it has antioxidant properties, by reducing oxidative stress, helping to maintain skin health, enhancing the skin's self-repair ability, and thus improving its antibacterial effect.

[0018] Although seaweed polyphenols have antibacterial potential, their effect when used alone is still limited. Mainly manifested in their poor antibacterial effect, and when they work, the concentration is relatively high, which may affect the sensory perception of cosmetics. At the same time, seaweed polyphenols will also lose their antibacterial activity during storage and use, showing instability. In order to improve seaweed polyphenols and antibacterial effects, the present invention combines a variety of seaweed polyphenols for the first time, and finds that under a specific ratio, the composition can make up for the shortcomings of a single component in antibacterial effect, give play to the complementarity of each component, and enhance the overall antibacterial activity.

[0019] Based on this, the present invention provides a composition with antibacterial effect, which is composed of a polyphenol extract of Porphyra haitanensis, a polyphenol extract of Dunaliella salina and a polyphenol extract of coralline algae, wherein the mass ratio of the polyphenol extract of Porphyra haitanensis, the polyphenol extract of Dunaliella salina and the polyphenol extract of coralline algae in the composition is (1~3):(1~3):(1~2), preferably, the mass ratio of the polyphenol extract of Porphyra haitanensis, the polyphenol extract of Dunaliella salina and the polyphenol extract of coralline algae is 3:1:1.

[0020] Experiments have shown that, compared with the seaweed polyphenol extract alone, the composition of the present invention can have a more significant inhibitory effect on Aureus coccus and Escherichia coli under the above-mentioned ratio conditions.

[0021] For the above composition, the preparation methods of the components thereof - the polyphenol extract of Porphyra haitanensis, the polyphenol extract of Dunaliella salina and the polyphenol extract of Coralline algae are as follows: S1. Weigh the powders of Porphyra haitanensis, Dunaliella salina and coral respectively, and mix them with ethanol solution respectively.

[0022] In some embodiments, the volume concentration of the ethanol solution is 40%-80%.

[0023] There are obvious differences in the amount of polyphenols extracted from the above three seaweed powders by ethanol solutions of different volume concentrations. According to the experimental results, the optimal ethanol volume concentration of Porphyra haitanensis is 60%, the optimal ethanol volume concentration of Dunaliella salina is 80%, and the optimal ethanol volume concentration of coral is 40%.

[0024] In some embodiments, the solid-liquid ratio of the above three powders to the ethanol solution is 1:8-12 (m / v).

[0025] S2. After mixing, subject it to ultrasonic treatment.

[0026] In some embodiments, the conditions for ultrasonic treatment are: temperature 30-50° C., ultrasonic power 60-100 W, and ultrasonic time 30-90 min.

[0027] S3. The obtained extract is centrifuged, and the supernatant is collected and concentrated.

[0028] In some embodiments, the centrifugation condition is: centrifugation at 5000 r / min for 10 min.

[0029] S4. The concentrated extract is mixed with the ethanol solution again.

[0030] In some embodiments, the second alcohol extraction adopts the optimal extraction conditions in S1. Specifically, the ethanol volume concentration used for the crude extract of Porphyra haitanensis is 60%, and the solid-liquid ratio is 1:10 (m / v); the ethanol volume concentration used for the crude extract of Dunaliella salina is 80%, and the solid-liquid ratio is 1:10 (m / v); the ethanol volume concentration used for the crude extract of coral is 40%, and the solid-liquid ratio is 1:10 (m / v).

[0031] S5. The extract after the second alcohol extraction is passed through an adsorption resin and purified to obtain the polyphenol extract of Porphyra haitanensis, the polyphenol extract of Dunaliella salina and the polyphenol extract of Corallina salina.

[0032] In some embodiments, the adsorption resin includes the macroporous adsorption resin LX-158.

[0033] In order to improve the purity of the seaweed polyphenol extract, the present invention selects to perform alcohol extraction again after concentration and purify the crude extract using an adsorption resin. The above method can avoid the adverse reactions caused by the low purity of seaweed polyphenols obtained by traditional extraction, which may affect the activity of the extract.

[0034] In some embodiments, the purification process using the above-mentioned macroporous adsorption resin LX-158 includes: concentrating the crude extract after the second alcohol extraction, and then adding the product to the macroporous adsorption resin LX-158 at a ratio of 30:1 (v / m), oscillating and adsorbing for 24 hours at 25°C and 100 r / min, filtering, and then adding ethanol of the corresponding extraction concentration at a ratio of 40:1 (v / m) and oscillating for 24 hours (100 r / min), separating the supernatant to obtain purified Porphyra haitanensis polyphenol extract, Dunaliella salina polyphenol extract and Coralline algae polyphenol extract.

[0035] The present invention adopts ultrasound-assisted ethanol extraction of the above-mentioned seaweed. Compared with the existing solvent extraction method and hot water extraction method, the extraction method of the present invention has higher extraction efficiency, can more fully extract the active ingredients in the seaweed polyphenols, and also avoids the degradation and loss of active ingredients caused by high temperature and long-term treatment, thereby improving the activity of the extract.

[0036] The above extraction method is used to obtain polyphenol extracts of Porphyra haitanensis, Dunaliella salina polyphenol extracts and coralline algae polyphenol extracts. The polyphenol structures of the three seaweed polyphenol extracts use phloroglucinol as the basic unit, and the molecular weight is about 126-650KDa. Compared with other plant polyphenols, the seaweed polyphenols in the present invention have a simple structure but diverse functions, and have multiple biological activities such as antioxidant, anti-inflammatory, and antibacterial. Therefore, the seaweed polyphenol extracts in the present invention can be applied to food, cosmetics and other fields.

[0037] Based on this, the present invention also provides the use of the above composition in the preparation of preservatives. By using the composition of the present invention as a preservative, the shelf life of the product can be significantly extended and microbial contamination can be reduced. Specifically, the preservatives prepared from the composition of the present invention include food preservatives and cosmetic preservatives.

[0038] When the above composition is used to prepare a food preservative, it can be added to different types of food (meat products, dairy products, fruits and vegetables, etc.) alone, or it can be compounded with other preservative ingredients that can be used in other foods and added to different types of food.

[0039] When the above composition is used to prepare a cosmetic preservative, it can be added to different types of cosmetics alone, or it can be added to different types of cosmetics after being compounded with other preservative ingredients that can be used in cosmetics. For example, a cosmetic preservative includes the above composition and other natural antibacterial ingredients, such as tea polyphenols, curcumin or citric acid.

[0040] At the same time, the present invention also provides the use of the above composition in the preparation of cosmetics. Specifically, the above cosmetics include types such as face creams, toners, facial masks, essences, and facial cleansers. The composition of the present invention can be added to different types of cosmetics alone, or the composition of the present invention can be compounded with other active ingredients and added to different types of cosmetics.

[0041] On the one hand, the cosmetics prepared by the composition of the present invention have the effect of removing acne. Experiments have shown that the composition of the present invention can effectively reduce the number of acne-related bacteria, alleviate skin inflammation, and improve skin condition.

[0042] On the other hand, the cosmetics prepared by using the composition of the present invention as an antioxidant, anti-inflammatory and moisturizing ingredient also have skin conditioning effects, can significantly improve skin quality, enhance skin barrier function, and reduce skin allergies and irritation reactions.

[0043] The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

[0044] Example 1 This embodiment is a composition with antibacterial effect and a preparation method thereof. The components of the composition are: polyphenol extract from Porphyra haitanensis, polyphenol extract from Dunaliella salina and polyphenol extract from coralline algae, and the mass ratio thereof is 3:1:1.

[0045] The preparation method of the above composition is as follows: (1) Weigh 5 g of seaweed powder into five 250 mL conical flasks and label them. Add ethanol solution in a solid-liquid ratio of 1:10 (m:v). The ethanol solution with a volume fraction of 60% was added to the Porphyra haitanensis powder, the ethanol solution with a volume fraction of 80% was added to the Dunaliella salina powder, and the ethanol solution with a volume fraction of 40% was added to the coral.

[0046] (2) After mixing, extract in an ultrasonic instrument at 40°C for 60 min according to the set parameters (ultrasonic power 80 W).

[0047] (3) The obtained extract was placed in a centrifuge and centrifuged at 5000 r / min for 10 min. The supernatant was collected and concentrated using a rotary evaporator to obtain a crude seaweed extract.

[0048] (4) Repeat the alcohol extraction treatment in step (1).

[0049] (5) Weigh an appropriate amount of LX-158 macroporous adsorption resin and soak it in 95% ethanol solution for 24 hours. Wash it with pure water until there is no alcohol smell before use.

[0050] (6) After the crude polyphenol extracts were concentrated by rotary evaporation, LX-158 macroporous adsorption resin was added at a ratio of 30:1 (v:m) and adsorbed at 25°C for 24 h (100 r / min). After filtration, the macroporous adsorption resin was washed with pure water, and ethanol of the corresponding extraction concentration was added at a ratio of 40:1 (v:m) and oscillated for 24 h (100 r / min). The supernatant was separated to obtain the purified polyphenol extract.

[0051] Comparative Example 1 The polyphenol extract of Porphyra haitanensis obtained by the extraction method of Example 1.

[0052] Comparative Example 2 The Dunaliella salina polyphenol extract was obtained by the extraction method of Example 1.

[0053] Comparative Example 3 The coralline algae polyphenol extract was obtained by the extraction method of Example 1.

[0054] Comparative Example 4 The difference from Example 1 is that the mass ratio of the polyphenol extract from Porphyra haitanensis, the polyphenol extract from Dunaliella salina and the polyphenol extract from Coralline algae is 1:1:1.

[0055] Comparative Example 5 The difference from Example 1 is that the mass ratio of the polyphenol extract of Porphyra haitanensis, the polyphenol extract of Dunaliella salina and the polyphenol extract of Coralline algae is 1:3:2.

[0056] Experimental Example 1 This experiment compares the effects of different alcohol extraction methods on the extraction amount of seaweed polyphenols. The specific extraction method refers to Example 1, and only the volume fraction of ethanol is changed.

[0057] The extracts were compared by DPPH scavenging test, the experimental method is as follows: 1. Preparation of DPPH ethanol solution: Weigh 8 mg DPPH, dissolve it in anhydrous ethanol and dilute it to a 100 mL volumetric flask to prepare 2×10 -4 mol / mL DPPH concentration; prepare and use immediately, store at 0-4℃ away from light, valid within 4 hours.

[0058] 2. Experimental steps: A: Take 100 μL of the test solution and 100 μL of 2×10 -4 mol / mL DPPH solution on a 96-well plate, set up 3 wells; B: Take 100 μL of anhydrous ethanol and 100 μL of 2×10 -4 mol / mL DPPH solution was placed on a 96-well plate and 3 wells were set; C: 100 μL of anhydrous ethanol and 100 μL of the test solution were placed on a 96-well plate and 1 well was set; after reacting for 30 min, the absorbance values ​​of A, B, and C were measured at 520 nm. The reagent ratio is shown in Table 1.

[0059] Table 1 DPPH free radical scavenging rate detection reagent ratio table

[0060] 3. Data processing The formula for calculating the clearance rate is: Clearance rate (%) = [(B+C)-A] / B.

[0061] 4. Experimental results and analysis The effects of different ethanol concentrations on the extraction of polyphenols from seven seaweeds were studied. The results are shown in Table 2.

[0062] Table 2 Polyphenol contents and DPPH scavenging rates of seven seaweed species

[0063] It can be seen from Table 2 that the DPPH scavenging rate of the coralline algae polyphenol extract containing a polyphenol concentration of 0.1 mg / g reaches the maximum at an ethanol concentration of 40%, with a maximum of 96.23%, and a maximum extraction amount of 1.94 mgGAE / gDM; the DPPH scavenging rate of the haitan laver polyphenol extract containing a polyphenol concentration of 0.1 mg / g reaches the maximum at an ethanol concentration of 60%, with a maximum of 77.06%, and a maximum extraction amount of 0.78 mgGAE / gDM; the DPPH scavenging rate of the Dunaliella salina polyphenol extract containing a polyphenol concentration of 0.1 mg / g reaches the maximum at an ethanol concentration of 80%, with a maximum of 56.26%, and a maximum extraction amount of 2.49 mgGAE / gDM; Among them, Dunaliella salina has the largest polyphenol extraction amount among the three brown algae.

[0064] Experimental Example 2 This experiment is a comparison of the Staphylococcus aureus inhibition experiment conducted by the composition obtained in Example 1 and the polyphenol extracts of Comparative Examples 1-5, and the specific steps are as follows: 1. Culture medium preparation: Mix LB broth and appropriate amount of pure water (solid-liquid ratio 1:25) in a beaker, heat on an induction cooker to dissolve until the liquid part is transparent and clear, put it into a sterilizer for sterilization and then bottle it.

[0065] 2. Bacterial recovery: Inoculate Staphylococcus aureus into the culture medium and culture it in a biochemical incubator at 37°C for 4-5 days.

[0066] 3. 96-well plate culture method: After the bacteria are grown, use PBS to make a spore suspension with an OD value of 1, then dilute it 3000 times with culture medium, add 100 μL of spore suspension and 100 μL of medium with a mass concentration of X mg·mL into a 96-well plate. -1 The polyphenol extract was added to a total of 200 μL of reaction solution. The control wells were 100 μL of polyphenol extract and 100 μL of culture medium, the positive control wells were 100 μL of spore suspension and 100 μL of ketoconazole inhibitor, and the negative control wells were 100 μL of spore suspension and 100 μL of culture medium. The negative Oh-OD value was measured immediately after the negative wells were mixed. There were 3 holes in each of the negative control wells, positive control wells, and test wells, and 1 control well, as shown in Tables 3 and 4. After the samples were mixed, the 96-well plate was placed in a 37°C biochemical incubator and cultured for 12 hours, and the OD600 value of each well was measured using an enzyme marker.

[0067] 4. Calculate the antibacterial rate:

[0068] Table 3 Antibacterial experiment of Staphylococcus aureus

[0069] Table 4 Gradient of polyphenol extract

[0070] 5. Experimental results: Antibacterial rate of seaweed polyphenols against Staphylococcus aureus Table 5 Inhibition rate of seaweed polyphenols against Staphylococcus aureus

[0071] The experimental results show that the combination has a significant inhibitory effect on Staphylococcus aureus, and the antibacterial effect is significantly better than that of a single seaweed polyphenol extract. Under the combination of Porphyra haitanensis: Dunaliella salina: coral mass ratio of 3:1:1, the IC50 is 0.406 mg / mL and the minimum inhibitory concentration is 0.420 mg / mL, which is the best combination in the combination formula.

[0072] Experimental Example 2 This experiment is a comparison of the Escherichia coli inhibition experiment of the composition obtained in Example 1 and the polyphenol extracts of Comparative Examples 1-5, and the specific steps are as follows: 1. Culture medium preparation: Mix LB broth and appropriate amount of pure water (solid-liquid ratio 1:25) in a beaker, heat on an induction cooker to dissolve until the liquid part is transparent and clear, put it into a sterilizer for sterilization and then bottle it.

[0073] 2. Bacterial recovery: Inoculate Escherichia coli into the culture medium and culture it in a biochemical incubator at 37°C for 3-4 days.

[0074] 3. 96-well plate culture method: After the bacteria are grown, use PBS to make a spore suspension with an OD value of 0.5, then dilute it 3000 times with culture medium, add 100 μL of spore suspension and 100 μL of medium with a mass concentration of X mg·mL into a 96-well plate. -1 The polyphenol extract was added to a total of 200 μL of reaction solution. The control wells were 100 μL of polyphenol extract and 100 μL of culture medium, the positive control wells were 100 μL of spore suspension and 100 μL of ketoconazole inhibitor, and the negative control wells were 100 μL of spore suspension and 100 μL of culture medium. The negative Oh-OD value was measured immediately after the negative wells were mixed. There were 3 holes in the negative control wells, positive control wells, and test wells, and 1 control well, as shown in Tables 9 and 10. After the samples were mixed, the 96-well plate was placed in a 37°C biochemical incubator and cultured for 12 hours, and the OD600 value of each well was measured using an enzyme reader. 4. Calculate the antibacterial rate:

[0075] Table 6 Escherichia coli antibacterial experiment

[0076] 5. Experimental results: Antibacterial rate of seaweed polyphenols and compound seaweed polyphenols on Escherichia coli From the results in Table 7, it can be seen that the seaweed extract polyphenol composition of Porphyra haitanensis: Dunaliella salina: Coralline algae = 3:1:1 has an excellent inhibitory effect on Escherichia coli, with a half inhibition concentration of 0.616 mg / mL and a minimum inhibitory concentration of 2.06 mg / mL.

[0077] Table 7 Antibacterial rate of seaweed polyphenols on Escherichia coli

[0078] The experimental results show that the combination has a significant inhibitory effect on Escherichia coli, and the antibacterial effect is significantly better than that of a single seaweed polyphenol extract. When the mass ratio of Porphyra haitanensis: Dunaliella salina: coral is 3:1:1, the IC50 is 0.616 mg / mL, which is the best combination in the combination formula.

[0079] Experimental Example 3 In this experiment, the composition obtained in Example 1 was used to prepare facial seaweed anti-acne essence, and the efficacy was evaluated. The specific steps are as follows: 1 The formula is shown in Table 8. Weigh butylene glycol, glycerol and transparent xanthan gum in A, stir evenly, add deionized water, trehalose, and EDTA-2Na to weigh the total volume and heat until the solution is completely transparent. Add water to the original weighed total volume, cool to 50°C, add dimethyl glycyrrhizinate and seaweed polyphenols and stir evenly. Cool to room temperature and add PE910.

[0080] 2 Efficacy evaluation: The product was applied to 20 volunteers with acne for 14 days, and an acne sensory evaluation was conducted on three aspects: the degree of acne reduction, improvement in skin condition, and skin feel.

[0081] Table 8 Seaweed Anti-Acne Essence Formula

[0082] 3. Experimental results: Evaluation of the efficacy of seaweed anti-acne essence Table 9 Sensory evaluation of seaweed anti-acne essence

[0083] From the results in Table 9, it can be seen that the seaweed extract polyphenol composition of Porphyra haitanensis: Dunaliella salina: Coralline algae = 3:1:1 can reduce the number of facial acne, restore the facial skin condition, and have a better skin feel. The composition of Example 1 can be used as an acne remover.

[0084] Experimental Example 4 In this experiment, the composition obtained in Example 1 was used to prepare seaweed facial gel and a sensory evaluation was performed. The specific steps are as follows: 1 The formula is shown in Table 10. Weigh U20 and sprinkle it evenly in a small amount of deionized water. Let it stand for 5 minutes, disperse and infiltrate until the white powder is completely wetted. In the infiltration system, weigh butylene glycol, glycerol and trehalose in turn, stir and heat to 80-85°C, stir for 5-10 minutes, rotate at 35-40 rpm, stir evenly and cool down. Weigh EDTA-2Na and seaweed polyphenol extract of Example 1 in turn, stir for 5-10 minutes, rotate at 35-40 rpm and dissolve until transparent for use. Cool down to 45°C, stir and mix evenly, then add NaOH (10%) to neutralize and stir to form a transparent gel. Add PE9010 and stir and mix evenly. Weigh after cooling to room temperature and put into a sterilized sample bottle. Weigh butylene glycol, glycerol and transparent xanthan gum in A, stir evenly, add deionized water, trehalose, and EDTA-2Na, weigh the total volume and heat until the solution is completely transparent. Add water to the original weighed total volume, cool to 50°C, add dimethyl glycyrrhizinate and seaweed polyphenols and stir evenly. Cool to room temperature and add PE910.

[0085] 2 Efficacy evaluation: 11 volunteers were given the product for 14 days, and an acne sensory evaluation was conducted on three aspects: the degree of acne reduction, improvement in skin condition, and skin feel.

[0086] Table 10 Seaweed facial gel formula

[0087] 3. Experimental results: Evaluation of the efficacy of seaweed facial gel Table 11 Sensory evaluation of seaweed facial gel

[0088] From the results in Table 11, it can be seen that the seaweed extract polyphenol composition of Porphyra haitanensis: Dunaliella salina: Coralline algae = 3:1:1 can reduce facial sensitivity, maintain skin moisture, and have a good skin feel. 11 volunteers were selected for skin testing, and more than 70% of the volunteers were satisfied with the seaweed gel. The composition of Example 1 can be used as a skin conditioner.

[0089] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A composition with antibacterial effect, characterized in that: The composition comprises: a polyphenol extract of Porphyra haitanensis, a polyphenol extract of Dunaliella salina and a polyphenol extract of coralline algae; the mass ratio of the polyphenol extract of Porphyra haitanensis, the polyphenol extract of Dunaliella salina and the polyphenol extract of coralline algae in the composition is (1-3):(1-3):(1-2).

2. The composition according to claim 1, characterized in that The mass ratio of the polyphenol extract of Porphyra haitanensis, the polyphenol extract of Dunaliella salina and the polyphenol extract of Coralline algae in the composition is 3:1:

1.

3. The method for preparing the composition according to claim 1 or 2, characterized in that: include: The raw materials are subjected to the first ethanol extraction, ultrasonic, concentration and purification treatment to obtain the polyphenol extract of Porphyra haitanensis, the polyphenol extract of Dunaliella salina and the polyphenol extract of Coralline algae, which are then mixed according to proportion.

4. The preparation method according to claim 3, characterized in that: The first ethanol extraction comprises: mixing the raw material with an ethanol solution having a volume concentration of 40%-60% according to a material-liquid ratio of 1:8-12 (m / v); The ultrasonic conditions are: temperature 30-50°C, ultrasonic power 60-100W, and ultrasonic time 30-90min; The purification includes: performing a second ethanol extraction on the concentrated product, and then passing the extract through a macroporous adsorption resin LX-158. The conditions of the second ethanol extraction are: the volume concentration of ethanol is 40%-60%, and the solid-liquid ratio is 1:8-12 (m / v).

5. Use of the composition as claimed in claim 1 or 2 in the preparation of a preservative.

6. The use according to claim 5, characterized in that: The preservative is used in food and cosmetics.

7. Use of the composition as claimed in claim 1 or 2 in the preparation of an antibacterial agent.

8. The use according to claim 7, characterized in that: The bacteria inhibited by the antibacterial agent include Escherichia coli and Staphylococcus aureus.

9. Use of the composition as claimed in claim 1 or 2 in the preparation of acne-removing and anti-inflammatory products.

10. Use of the composition according to claim 1 or 2 in the preparation of skin conditioning products.

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