Composition containing folium cortex eucommiae extract and application of composition in food for old dogs

Abstract

The invention relates to the field of pet food, and discloses a composition containing a folium cortex eucommiae extract and application of the composition in food for old dogs. The composition containing the folium cortex eucommiae extract is prepared from 10 to 20 parts of folium cortex eucommiae extract, 15 to 30 parts of radix astragali seu hedysari extract, 15 to 30 parts of fructus lycii extract, 100 to 250 parts of fruit powder, 100 to 250 parts of vegetable powder and 60 to 200 parts of enzymolysis fresh chicken powder. The composition is applied to preparation of food for old dogs, and the addition amount is 2-3% (by weight). According to the food for the elderly dogs, the composition containing the folium cortex eucommiae extract serves as a key additive, the chronic inflammation of the pet dogs can be relieved, free radical oxidation damage of the pet dogs can be reduced, the immunity is improved, and the disease risk is reduced.

Description

Technical Field

[0001] The present application relates to the field of pet food, and more specifically, to a composition containing eucommia leaf extract and application thereof in elderly dog ​​food. Background Art

[0002] As the most loyal emotional sustenance of human beings, the health and longevity of pets are becoming the good wishes of pet owners. Depending on factors such as breed, body shape and living environment, the average lifespan of dogs is between 7 and 18 years. With the stability and development of the pet economy, more and more pet dogs are gradually aging and entering the middle-aged and elderly stage. Aging refers to the process of degenerative changes that slowly appear and generally occur in various tissues and organs of the body with age. For pets, specific manifestations include graying hair, slow reaction, poor appetite, drowsiness, cloudy eyes, weakened digestive function, etc.

[0003] There are many reasons for the aging process of pets, including cellular, metabolic, and immune levels. The metabolic and immune levels can be intervened through diet. As the main source of nutrition and energy for pets, pet staple food is the most direct and convenient way to regulate the health of pets.

[0004] Therefore, it is urgent to develop a pet food that can relieve chronic inflammation in pet dogs, reduce their free radical oxidative damage, and enhance their immune capacity. Summary of the invention

[0005] The present invention aims to provide a composition which can relieve chronic inflammation of pet dogs, reduce free radical oxidative damage and improve immunity, and apply the composition to high-puffed dog food to counteract the aging degree of pet dogs.

[0006] The following is an explanation of the mechanism by which free radical oxidative damage and chronic inflammation lead to aging:

[0007] The pet's body produces free radicals during normal metabolism, and these free radicals are highly oxidative. If there are too many free radicals in the body, it will trigger an oxidative stress response, damage biological macromolecules in the cells, such as DNA, proteins, and lipids, and accelerate cell aging. Antioxidants can neutralize free radicals and reduce oxidative stress. Pets can obtain antioxidants from food, such as vitamin C, vitamin E, and carotenoids. In addition, some endogenous antioxidant enzymes, such as superoxide dismutase (SOD), also play an important role in regulating oxidative stress. By maintaining redox balance in the body, the aging of pets can be delayed.

[0008] The immune system of pets will also age with age, which is manifested by decreased immune cell function and weakened immune response ability. For example, the activity of T cells and B cells of elderly pets will decrease, and their ability to identify and eliminate pathogens will be weakened. Studies have found that through some immune regulation methods, such as the use of specific immune enhancers, the immune system of pets can be stimulated, the activity of immune cells can be increased, and their resistance to diseases can be enhanced, thereby delaying the overall aging process caused by the aging of the immune system.

[0009] Chronic inflammation plays an important role in the aging process of pets. As they age, the level of inflammatory factors in their bodies increases, and this chronic inflammatory state can damage tissues and organs. Controlling inflammatory responses can be done through dietary adjustments, such as reducing the intake of foods rich in inflammatory factors and increasing the intake of foods with anti-inflammatory effects, such as fish oil rich in omega-3 fatty acids. In addition, some natural anti-inflammatory substances, such as curcumin, can also regulate inflammatory responses in pets and delay aging.

[0010] Based on the above, in order to achieve the invention purpose of this application, this application adopts the following technical solutions:

[0011] In a first aspect, the present application provides a composition containing eucommia leaf extract, which includes the following components by mass: 10 to 20 parts of eucommia leaf extract, 15 to 30 parts of astragalus extract, 15 to 30 parts of wolfberry extract, 100 to 250 parts of fruit powder, 100 to 250 parts of vegetable powder, and 60 to 200 parts of enzymatic fresh chicken powder.

[0012] Furthermore, the fruit powder comprises the following components by weight: 10 to 20 parts of kiwi powder, 10 to 15 parts of raspberry powder, and 8 to 18 parts of apple powder.

[0013] Furthermore, the vegetable powder comprises the following components by weight: 8 to 20 parts of kale, 10 to 22 parts of carrots, and 10 to 15 parts of spinach.

[0014] Furthermore, the preparation method of the Eucommia ulmoides leaf extract is:

[0015] The eucommia leaves are washed to remove surface impurities, dried and crushed to obtain eucommia leaf powder;

[0016] Mix Eucommia leaf powder with water, soak at 50-60°C for 20-30 minutes, then heat to 98-100°C for extraction for 30-40 minutes;

[0017] Cool down to 40-50°C, and extract by ultrasonic at a frequency of 40-42 kHz for 30-40 min;

[0018] Filter and remove solid impurities to obtain an extract, which is then concentrated, dried, and crushed to obtain an eucommia leaf extract.

[0019] Furthermore, the preparation method of the astragalus extract is:

[0020] Wash the astragalus to remove surface impurities, dry and crush to obtain astragalus powder;

[0021] Add astragalus powder to 10 to 12 times the amount of water and soak for 10 to 12 hours;

[0022] Using 70-80% ethanol as solvent, extract at 80-85°C for 2-3h, extract three times in total, and combine the extracts;

[0023] The astragalus extract is obtained by filtering, concentrating, purifying, drying and crushing.

[0024] Furthermore, the preparation method of the wolfberry extract is:

[0025] The wolfberries are baked at low temperature, ripened, and crushed to obtain wolfberry powder;

[0026] Add wolfberry powder to 10-12 times the amount of citric acid buffer with a pH value of 6.5, and heat to 95°C to inactivate for 20-30 minutes;

[0027] Add 0.1-1% of cellulase by weight of wolfberry powder, perform enzymolysis at 55°C for 3-4 hours, centrifuge to obtain a clarified solution;

[0028] The mixture was sterilized in a 95°C water bath for 10-20 min, centrifuged, concentrated, dried and crushed to obtain wolfberry extract.

[0029] Furthermore, the preparation method of the enzymatically hydrolyzed fresh chicken powder is:

[0030] Take fresh chicken breast, wash and remove impurities, mince, add appropriate amount of water and adjust the pH to 6.0-6.5, heat to 55-60℃ and keep for 20-30min;

[0031] Add 0.2-0.4% (by weight) of papain to the raw material, and perform enzymolysis at 55-60° C. for 4-6 hours;

[0032] Heat to 90-92°C and maintain for 10-20 minutes;

[0033] Filter, concentrate, dry and crush to obtain enzymatically hydrolyzed fresh chicken powder.

[0034] In a second aspect, the present application provides the use of the composition containing the eucommia leaf extract described in the first aspect in preparing food for elderly dogs, wherein the addition amount of the composition containing the eucommia leaf extract is 2 to 3% (by weight).

[0035] In a third aspect, the present application provides a food for elderly dogs, comprising the following components by weight: 15 to 35 parts of fresh chicken, 15 to 23 parts of chicken meal, 15 to 20 parts of dehydrated beef, 3 to 8 parts of fresh chicken liver, 8 to 15 parts of sweet potato granules, 5 to 10 parts of peas, 5 to 10 parts of rice protein powder, 2 to 5 parts of chicken fat, 1 to 3 parts of salmon oil, 1 to 3 parts of beet pulp, 1 to 2 parts of inulin, 0.2 to 0.5 parts of yucca powder, and 2 to 3 parts of multivitamin and multi-mineral compound packages;

[0036] It also includes a composition containing the eucommia leaf extract described in the first aspect, accounting for 2-3% (by weight) of the raw material.

[0037] In a fourth aspect, the present application provides a method for preparing the elderly dog ​​food described in the third aspect, comprising the following steps:

[0038] Dehydrate the fresh chicken, and then grind all the raw materials separately to a particle size of 1.2 to 1.5 mm;

[0039] The crushed raw materials are mixed and stirred, and the moisture content is controlled at 25-30%, the temperature is 105-115℃, and the pressure is 35-40kg / cm 2 , the screw speed is 220-240 rpm, the die head temperature is 120-130°C, and the elderly dog ​​food is extruded.

[0040] In summary, this application has the following beneficial effects:

[0041] The composition of the present application, wherein the main active ingredients of the Eucommia leaf extract are chlorogenic acid, flavonoids, etc., have pharmacological effects such as anti-oxidation, anti-inflammatory, free radical scavenging, immune regulation, anti-cancer and anti-aging, can enhance the immunity of elderly dogs and reduce the risk of diseases; the main active ingredient of the Astragalus extract is Astragalus polysaccharide, which has multiple functions such as stimulating cell division and differentiation, improving the body's immunity, improving blood circulation, and regulating endocrine balance; the wolfberry extract is rich in antioxidants, such as polyphenols, flavonoids, vitamin C, etc., which can help scavenge free radicals and reduce oxidative damage, and is also rich in carotene, zinc, iron, copper, etc., which can enhance immunity, improve sleep, improve mental state, delay the aging process, and can further enhance the physique and resistance of elderly dogs; the fruit and vegetable powder is rich in vitamin C, vitamin E, carotene, etc., which can slow down oxidative damage, and the rich dietary fiber can also promote intestinal health and help the body detoxify.

[0042] The elderly dog ​​food of the present application uses a composition containing eucommia leaf extract as a key additive, which can relieve chronic inflammation of pet dogs, reduce their free radical oxidative damage, improve immunity, and reduce disease risks.

[0043] The senior dog food of the present application also contains high-quality protein sources such as fresh chicken and chicken meal, as well as a variety of carbohydrates such as sweet potato granules, peas, and rice protein powder, providing comprehensive nutritional support for senior dogs; at the same time, the addition of animal fats such as chicken oil and salmon oil not only increases the taste and flavor of the food, but also provides essential fatty acids, which helps maintain the health of the skin and hair of senior dogs. DETAILED DESCRIPTION

[0044] The technical scheme and effects of the present application are further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the invention, rather than to limit the invention.

[0045] It is worth noting that the raw materials used in this application are all commercially available unless otherwise specified; the methods used in this application are all existing methods unless otherwise specified.

[0046] Preparation Example 1: Eucommia ulmoides leaf extract

[0047] This preparation example discloses an eucommia leaf extract, which is prepared by the following method:

[0048] The eucommia leaves are washed to remove surface impurities, dried and crushed to obtain eucommia leaf powder;

[0049] Eucommia leaf powder was mixed with water, soaked at 50°C for 30 min, and then heated to 98°C for extraction for 40 min;

[0050] Cool down to 40°C and extract by ultrasound at a frequency of 40kHz for 30min;

[0051] Filter and remove solid impurities to obtain an extract, which is then concentrated, dried, and crushed to obtain an eucommia leaf extract.

[0052] Preparation Example 2: Eucommia ulmoides leaf extract

[0053] This preparation example discloses an eucommia leaf extract, which is prepared by the following method:

[0054] The eucommia leaves are washed to remove surface impurities, dried and crushed to obtain eucommia leaf powder;

[0055] Eucommia leaf powder was mixed with water, soaked at 55°C for 25 min, and then heated to 99°C for extraction for 35 min;

[0056] Cool down to 45°C and extract by ultrasound at a frequency of 41kHz for 35min;

[0057] Filter and remove solid impurities to obtain an extract, which is then concentrated, dried, and crushed to obtain an eucommia leaf extract.

[0058] Preparation Example 3: Eucommia ulmoides leaf extract

[0059] This preparation example discloses an eucommia leaf extract, which is prepared by the following method:

[0060] The eucommia leaves are washed to remove surface impurities, dried and crushed to obtain eucommia leaf powder;

[0061] Mix the Eucommia leaf powder with water, soak at 60°C for 20 min, then heat to 100°C and extract for 30 min;

[0062] Cool down to 50°C and extract by ultrasound at a frequency of 42kHz for 30min;

[0063] Filter and remove solid impurities to obtain an extract, which is then concentrated, dried, and crushed to obtain an eucommia leaf extract.

[0064] Preparation Example 4: Astragalus Extract

[0065] This preparation example discloses an Astragalus extract, which is prepared by the following method:

[0066] Wash the astragalus to remove surface impurities, dry and crush to obtain astragalus powder;

[0067] Add astragalus powder to 10 times the amount of water and soak for 12 hours;

[0068] Using 70% ethanol as solvent, extract at 80°C for 3 h, extract three times in total, and combine the extracts;

[0069] The astragalus extract is obtained by filtering, concentrating, purifying, drying and crushing.

[0070] Preparation Example 5: Astragalus Extract

[0071] This preparation example discloses an Astragalus extract, which is prepared by the following method:

[0072] Wash the astragalus to remove surface impurities, dry and crush to obtain astragalus powder;

[0073] Add astragalus powder to 11 times the amount of water and soak for 11 hours;

[0074] Using 75% ethanol as solvent, extract at 82°C for 2.5 h for 3 times and combine the extracts;

[0075] The astragalus extract is obtained by filtering, concentrating, purifying, drying and crushing.

[0076] Preparation Example 6: Astragalus Extract

[0077] This preparation example discloses an Astragalus extract, which is prepared by the following method:

[0078] Wash the astragalus to remove surface impurities, dry and crush to obtain astragalus powder;

[0079] Add astragalus powder to 12 times the amount of water and soak for 10 hours;

[0080] 80% ethanol was used as the solvent, and the extraction was performed at 85°C for 2 h, for a total of 3 times, and the extracts were combined;

[0081] The astragalus extract is obtained by filtering, concentrating, purifying, drying and crushing.

[0082] Preparation Example 7: Enzymatic hydrolysis of fresh chicken powder

[0083] This preparation example discloses an enzymatically hydrolyzed fresh chicken powder, which is prepared by the following method:

[0084] Take fresh chicken breast, wash and remove impurities, mince, add appropriate amount of water and adjust the pH to 6.0-6.5, heat to 55℃ and keep for 30min;

[0085] Add 0.2% (by weight) papain to the raw material and perform enzymolysis at 55°C for 6 hours;

[0086] Heat to 90°C for 20 min;

[0087] Filter, concentrate, dry and crush to obtain enzymatically hydrolyzed fresh chicken powder.

[0088] Preparation Example 8: Enzymatic hydrolysis of fresh chicken powder

[0089] This preparation example discloses an enzymatically hydrolyzed fresh chicken powder, which is prepared by the following method:

[0090] Take fresh chicken breast, wash and remove impurities, mince, add appropriate amount of water and adjust the pH to 6.0-6.5, heat to 58℃ and keep for 25min;

[0091] Add 0.3% (by weight) papain to the raw material and perform enzymolysis at 58°C for 5h;

[0092] Heat to 91°C for 15 min;

[0093] Filter, concentrate, dry and crush to obtain enzymatically hydrolyzed fresh chicken powder.

[0094] Preparation Example 9: Enzymatic hydrolysis of fresh chicken powder

[0095] This preparation example discloses an enzymatically hydrolyzed fresh chicken powder, which is prepared by the following method:

[0096] Take fresh chicken breast, wash and remove impurities, mince, add appropriate amount of water and adjust the pH to 6.0-6.5, heat to 60℃ and keep for 20min;

[0097] Add 0.4% (by weight) papain to the raw material and perform enzymolysis at 60°C for 4 hours;

[0098] Heat to 92°C for 10 min;

[0099] Filter, concentrate, dry and crush to obtain enzymatically hydrolyzed fresh chicken powder.

[0100] Preparation Example 10: Lycium barbarum extract

[0101] This preparation example discloses a wolfberry powder, which is prepared by the following method:

[0102] The wolfberries are baked at low temperature, ripened, crushed, and passed through a 60-mesh sieve to obtain wolfberry powder;

[0103] Add wolfberry powder to 10 times the amount of citric acid buffer with a pH value of 6.5, and heat to 95°C to inactivate for 20 to 30 minutes;

[0104] Add 0.1% of cellulase based on the weight of wolfberry powder, perform enzymolysis at 55°C for 3 hours, and centrifuge to obtain a clarified solution;

[0105] The mixture was sterilized in a 95°C water bath for 10 min, centrifuged, concentrated, dried and crushed to obtain the wolfberry extract.

[0106] Preparation Example 11: Lycium barbarum extract

[0107] This preparation example discloses a wolfberry powder, which is prepared by the following method:

[0108] The wolfberries are baked at low temperature, ripened, crushed, and passed through a 60-mesh sieve to obtain wolfberry powder;

[0109] Add wolfberry powder to 11 times the amount of citric acid buffer with a pH value of 6.5, and heat to 95°C to inactivate for 20 to 30 minutes;

[0110] Add 0.5% of cellulase by weight of wolfberry powder, perform enzymolysis at 55°C for 3.5 hours, centrifuge, and obtain a clarified solution;

[0111] The mixture was sterilized in a 95°C water bath for 15 min, centrifuged, concentrated, dried and crushed to obtain the wolfberry extract.

[0112] Preparation Example 12: Lycium barbarum extract

[0113] This preparation example discloses a wolfberry powder, which is prepared by the following method:

[0114] The wolfberries are baked at low temperature, ripened, crushed, and passed through a 60-mesh sieve to obtain wolfberry powder;

[0115] Add wolfberry powder to 12 times the amount of citric acid buffer with a pH value of 6.5, and heat to 95°C to inactivate for 20 to 30 minutes;

[0116] Add 1% of cellulase by weight of wolfberry powder, perform enzymolysis at 55°C for 4 hours, and centrifuge to obtain a clarified solution;

[0117] The mixture was sterilized in a 95°C water bath for 20 min, centrifuged, concentrated, dried and crushed to obtain the wolfberry extract.

[0118] Preparation Example 13: Fruit Powder

[0119] This preparation example discloses a fruit powder, which is obtained by mechanically grinding the following raw materials in parts by weight:

[0120] 10 parts kiwi powder, 10 parts raspberry powder, 8 parts apple powder.

[0121] Preparation Example 14: Fruit Powder

[0122] This preparation example discloses a fruit powder, which is obtained by mechanically grinding the following raw materials in parts by weight:

[0123] 15 parts of kiwi powder, 12.5 parts of raspberry powder, and 13 parts of apple powder.

[0124] Preparation Example 15: Fruit Powder

[0125] This preparation example discloses a fruit powder, which is obtained by mechanically grinding the following raw materials in parts by weight:

[0126] 20 parts kiwi powder, 15 parts raspberry powder, 18 parts apple powder.

[0127] Preparation Example 16: Vegetable Powder

[0128] This preparation example discloses a vegetable powder, which is obtained by mechanically grinding the following raw materials in parts by weight:

[0129] 8 parts kale, 10 parts carrots, 10 parts spinach.

[0130] Preparation Example 17: Vegetable Powder

[0131] This preparation example discloses a vegetable powder, which is obtained by mechanically grinding the following raw materials in parts by weight:

[0132] 14 portions of kale, 16 portions of carrots, and 12.5 portions of spinach.

[0133] Preparation Example 18: Vegetable Powder

[0134] This preparation example discloses a vegetable powder, which is obtained by mechanically grinding the following raw materials in parts by weight:

[0135] 20 portions of kale, 22 portions of carrots, and 15 portions of spinach.

[0136] Example 1-3: Composition containing Eucommia ulmoides leaf extract

[0137] This example discloses a composition containing an extract of Eucommia ulmoides leaves, the raw materials used for its preparation are shown in the following table, and the composition is obtained by mechanical mixing.

[0138] Example 1 Example 2 Example 3 Eucommia ulmoides leaf extract (Preparation Example 1) 10 15 20 Astragalus extract (Preparation Example 4) 15 22 30 Lycium barbarum extract (Preparation Example 10) 15 23 30 Fruit powder (Preparation Example 13) 100 175 250 Vegetable powder (Preparation Example 16) 100 175 250 Enzymatic hydrolysis of fresh chicken powder (preparation example 7) 60 130 200

[0139] Example 4-8: Composition containing Eucommia ulmoides leaf extract

[0140] This embodiment discloses a composition containing an extract of Eucommia ulmoides leaves, which is different from that of Example 2 in that the preparation process of each raw material is different (the addition amount is the same), as shown in the following table.

[0141]

[0142]

[0143] Application example 1-3: Dog food for the elderly

[0144] This application example discloses a food for elderly dogs, and the raw materials used in its preparation are shown in the following table.

[0145]

[0146] The preparation method of the elderly dog ​​food comprises: dehydrating fresh chicken, and then crushing all raw materials respectively, with the crushing particle size being 1.2-1.5 mm;

[0147] The crushed raw materials are mixed and stirred, and the moisture content is controlled at 28%, the temperature is 110℃, and the pressure is 35-40kg / cm 2 , the screw speed is 235 rpm, the die temperature is 130°C, and the elderly dog ​​food is extruded.

[0148] Application example 4-5: Dog food for the elderly

[0149] This application example discloses a food for elderly dogs, and the raw materials used in its preparation are shown in the following table.

[0150]

[0151] The preparation method of the elderly dog ​​food comprises: dehydrating fresh chicken, and then crushing all raw materials respectively, with the crushing particle size being 1.2-1.5 mm;

[0152] The crushed raw materials are mixed and stirred, and the moisture content is controlled at 28%, the temperature is 110℃, and the pressure is 35-40kg / cm 2, the screw speed is 235 rpm, the die temperature is 130°C, and the elderly dog ​​food is extruded.

[0153] Application example 6-12: Dog food for the elderly

[0154] This application example discloses a food for elderly dogs, which is different from application example 4 only in that the preparation process of the composition containing eucommia leaf extract is different (the addition amount is the same), and the corresponding relationship is shown in the following table.

[0155]

[0156]

[0157] Comparative Example 1: Dog Food

[0158] This comparative example discloses a dog food, which is different from Application Example 1 in that the composition containing eucommia leaf extract is not added.

[0159] Comparative Example 2: Dog Food

[0160] This comparative example discloses a dog food, which is different from Application Example 1 in that, during the preparation process of the composition containing eucommia leaf extract as a raw material, eucommia leaf extract is not added.

[0161] Comparative Example 3: Dog Food

[0162] This comparative example discloses a dog food, which is different from Application Example 1 in that no astragalus extract is added during the preparation of the composition containing eucommia leaf extract as a raw material.

[0163] Comparative Example 4: Dog Food

[0164] This comparative example discloses a dog food, which is different from Application Example 1 in that, during the preparation of the composition containing eucommia leaf extract as a raw material, no wolfberry extract is added.

[0165] Performance Testing

[0166] (1) Test subjects:

[0167] 30 healthy small and medium-sized elderly dogs were randomly divided into 5 groups, with 6 dogs in each group, and were fed with the dog food of Application Example 1 and Comparative Examples 1-4 respectively.

[0168] (2) Tracking indicators:

[0169] ① Food intake: By recording each dog’s daily food intake, the palatability of the pet food and the dog’s acceptance can be assessed.

[0170] ② Stool score: Preliminary assessment of the digestibility of pet food by observing the shape, color and texture of the stool (1-5 points, 3.5-4 points for ideal health);

[0171] Fecal Scoring Criteria:

[0172] 1 point: the stool is completely liquid (no substantial solid structure);

[0173] 1.5 points, liquid stool with low viscosity;

[0174] 2 points: the stool is relatively moist but not liquid, with some moisture remaining and visible outside the stool;

[0175] 2.5 points: the stool is moist, has a low consistency and is not formed. The stool can hold water without any water floating on the surface.

[0176] 3 points: the stool is moist, without cracks, with clear shapes, and the different "components" of the stool are adhered to each other;

[0177] 3.5 points: the stool is wet and begins to lose its shape and crack, and the "components" of the stool separate and the cracks are visible;

[0178] 4 points: the feces have clearly visible shapes and cracks, and only a small amount remains on the ground when the feces is picked up;

[0179] 4.5 points: the feces has clearly visible cracks, is extremely dry on the outside and relatively dry on the inside, and there is no residue on the ground when it is picked up;

[0180] 5 points: hard, dry and friable stool (lumpy) that cracks easily.

[0181] ③Inflammation indicators:

[0182] C-reactive protein (CRP): Detect the CRP concentration in dog serum to evaluate the effect of pet food on the inflammatory response in dogs;

[0183] Interleukin-6 (IL-6): Detect IL-6 levels in dog serum to evaluate the impact of pet food on the inflammatory process in dogs.

[0184] ④Antioxidant index:

[0185] Superoxide dismutase (SOD): Detect SOD activity in dog serum to evaluate the antioxidant properties of pet food.

[0186] Glutathione peroxidase (GSH-Px): Detect the GSH-Px activity in dog serum and evaluate the effect of pet food on the antioxidant enzyme system in dogs.

[0187] Malondialdehyde (MDA): Detect the MDA content in dog serum or feces to evaluate the impact of pet food on fat oxidation in dogs.

[0188] ⑤Immune indicators:

[0189] Immunoglobulin G (IgG): Detect the IgG level in dog serum to evaluate the effect of pet food on dog humoral immune function.

[0190] Immunoglobulin M (IgM): I detects the level of IgM in dog serum to evaluate the effect of pet food on the dog's early immune response.

[0191] Immunoglobulin A (IgA): Detect the IgA level in dog saliva or feces to evaluate the impact of pet food on dog mucosal immune function.

[0192] (3) Test results:

[0193]

[0194]

[0195] In addition, the above method was also used to test the elderly dog ​​food prepared in Example 2-12, and the results were similar to those in Example 1.

[0196] In summary, dog food containing Eucommia leaf extract can significantly improve the fecal quality of dogs, reduce the level of inflammation in the body, and improve antioxidant capacity and humoral immunity. Therefore, the dog food containing Eucommia leaf extract can be used as an effective feed choice to relieve chronic inflammation in pet dogs, reduce free radical oxidative damage, and improve immunity.

[0197] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A composition containing an extract of Eucommia ulmoides leaves, characterized in that: The invention comprises the following components by weight: 10-20 parts of eucommia leaf extract, 15-30 parts of astragalus extract, 15-30 parts of wolfberry extract, 100-250 parts of fruit powder, 100-250 parts of vegetable powder and 60-200 parts of enzymatic fresh chicken powder.

2. The composition containing the Eucommia ulmoides leaf extract according to claim 1, characterized in that The fruit powder comprises the following components by weight: 10 to 20 parts of kiwi fruit powder, 10 to 15 parts of raspberry powder, and 8 to 18 parts of apple powder.

3. The composition containing the Eucommia ulmoides leaf extract according to claim 1, characterized in that The vegetable powder comprises the following components by weight: 8 to 20 parts of kale, 10 to 22 parts of carrots, and 10 to 15 parts of spinach.

4. The composition containing the Eucommia ulmoides leaf extract according to claim 1, characterized in that The preparation method of the Eucommia ulmoides leaf extract is: The eucommia leaves are washed to remove surface impurities, dried and crushed to obtain eucommia leaf powder; Mix Eucommia leaf powder with water, soak at 50-60°C for 20-30 minutes, then heat to 98-100°C for extraction for 30-40 minutes; Cool down to 40-50°C, and extract by ultrasonic at a frequency of 40-42 kHz for 30-40 min; Filter and remove solid impurities to obtain an extract, which is then concentrated, dried, and crushed to obtain an eucommia leaf extract.

5. The composition containing the Eucommia ulmoides leaf extract according to claim 1, characterized in that The preparation method of the astragalus extract is: Wash the astragalus to remove surface impurities, dry and crush to obtain astragalus powder; Add astragalus powder to 10 to 12 times the amount of water and soak for 10 to 12 hours; Using 70-80% ethanol as solvent, extract at 80-85°C for 2-3h, extract three times in total, and combine the extracts; The astragalus extract is obtained by filtering, concentrating, purifying, drying and crushing.

6. The composition containing the Eucommia ulmoides leaf extract according to claim 1, characterized in that The preparation method of the wolfberry extract is: The wolfberries are baked at low temperature, ripened, and crushed to obtain wolfberry powder; Add wolfberry powder to 10-12 times the amount of citric acid buffer with a pH value of 6.5, and heat to 95°C to inactivate for 20-30 minutes; Add 0.1-1% of cellulase by weight of wolfberry powder, perform enzymolysis at 55°C for 3-4 hours, centrifuge to obtain a clarified solution; The mixture was sterilized in a 95°C water bath for 10-20 min, centrifuged, concentrated, dried and crushed to obtain wolfberry extract.

7. The composition containing the Eucommia ulmoides leaf extract according to claim 1, characterized in that The preparation method of the enzymatically hydrolyzed fresh chicken powder is: Take fresh chicken breast, wash and remove impurities, mince, add appropriate amount of water and adjust the pH to 6.0-6.5, heat to 55-60℃ and keep for 20-30min; Add 0.2-0.4% (by weight) of papain to the raw material, and perform enzymolysis at 55-60° C. for 4-6 hours; Heat to 90-92°C and maintain for 10-20 minutes; Filter, concentrate, dry and crush to obtain enzymatically hydrolyzed fresh chicken powder.

8. Use of the composition containing the Eucommia leaf extract according to any one of claims 1 to 7 in preparing food for elderly dogs, characterized in that: The added amount of the composition containing the eucommia leaf extract is 2-3% (by weight).

9. A food for elderly dogs, characterized in that: The ingredients include the following by weight: 15-35 parts of fresh chicken, 15-23 parts of chicken meal, 15-20 parts of dehydrated beef, 3-8 parts of fresh chicken liver, 8-15 parts of sweet potato granules, 5-10 parts of peas, 5-10 parts of rice protein powder, 2-5 parts of chicken fat, 1-3 parts of salmon oil, 1-3 parts of beet pulp, 1-2 parts of inulin, 0.2-0.5 parts of yucca powder, and 2-3 parts of multivitamin and multi-mineral compound packages; The invention also comprises a composition containing the eucommia leaf extract according to any one of claims 1 to 7, accounting for 2 to 3% (by weight) of the raw material.

10. The method for preparing the elderly dog ​​food according to claim 9, characterized in that: The following steps are involved: Dehydrate the fresh chicken, and then grind all the raw materials separately to a particle size of 1.2 to 1.5 mm; The crushed raw materials are mixed and stirred, and the moisture content is controlled at 25-30%, the temperature is 105-115℃, and the pressure is 35-40kg / cm 2 , the screw speed is 220-240 rpm, the die head temperature is 120-130°C, and the elderly dog ​​food is extruded.

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