Selenium-enriched malt flour and preparation method thereof
By introducing selenium-rich bacterial solution for co-culture and heat treatment during wheat preparation, the problem of inorganic selenium in the prior art is solved, the organic selenium content in selenium-rich malt powder is improved, and better selenium supplementation effect and the development of functional foods are achieved.
Patent Information
- Application Number
- CN202510043504.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-10
- Publication Date
- 2025-03-21
AI Technical Summary
In the existing process for preparing selenium malt, selenium is mainly in the form of inorganic selenium, which is not conducive to the absorption and utilization of human body, resulting in a reduction in the selenium supplement effect of selenium malt powder, and the organic selenium content in malt is low and the process efficiency is low.
Selenium-rich malt powder was obtained by soaking the wheat and mixing it with selenium-rich bacterial solution, co-culture and heat treatment. This method introduces Bacillus spindle-shaped lysine and Streptococcus thermophilus to coordinate the effect to improve the utilization of selenium and convert inorganic selenium into organic selenium.
It improves the organic selenium content in selenium-rich malt powder, enhances the selenium supplement effect of the product, has the effect of anti-tumor and nodules, provides raw materials for the development of functional foods, and improves the nutritional and health care value of the product.
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Abstract
Description
Technical Field
[0001] The invention relates to the technical field of selenium-enriched malt powder, in particular to selenium-enriched malt powder and a preparation method thereof. Background Art
[0002] 50 micrograms of selenium is an essential trace element for the human body every day. Selenium can enhance the body's immune function, anti-oxidation, delay aging, and effectively inhibit tumor growth. It has a good auxiliary improvement effect on patients after surgery and radiotherapy and chemotherapy.
[0003] Selenium malt can serve as an active carrier for selenium transformation. After absorbing inorganic selenium, it is absorbed and transformed through the germination process, so that selenium is enriched in the amino acids, proteins and other molecules contained in the malt, making it easier for the human body to absorb and utilize it.
[0004] At present, the process for preparing selenium malt is mainly to soak wheat in a solution containing inorganic selenium, so that the wheat absorbs selenium, and then obtain selenium malt through germination. However, the selenium in the selenium malt obtained by this method mainly exists in the form of inorganic selenium, and only a small part is converted into organic selenium in malt, and inorganic selenium is not conducive to human absorption and utilization, which reduces the selenium supplementation effect of selenium malt powder. In addition, this type of method is not conducive to the absorption of inorganic selenium by malt, which is also an important reason for the low content of organic selenium in malt, so that the overall process efficiency needs to be further improved. Summary of the invention
[0005] The purpose of the present invention is to provide a selenium-enriched malt powder and a preparation method thereof, which utilizes different strains to improve the utilization rate of selenium, and at the same time adjusts the existing preparation process to efficiently prepare the selenium-enriched malt powder.
[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:
[0007] The present invention provides a method for preparing selenium-enriched malt powder, comprising the following steps:
[0008] (1) soaking the wheat and then draining it to obtain soaked wheat;
[0009] (2) mixing the soaked wheat and the selenium-enriched bacterial solution to obtain a mixture;
[0010] (3) heat treating the mixed material after co-culturing to obtain malt;
[0011] (4) The malt is cleaned, roasted, and crushed to obtain selenium-enriched malt powder.
[0012] Preferably, the wheat is soaked in a sodium selenite aqueous solution in step (1), the mass ratio of the wheat to the sodium selenite aqueous solution is 1:6-8, the concentration of sodium selenite in the sodium selenite aqueous solution is 280-320 mg / L, and the soaking time is 2-6 h.
[0013] Preferably, the selenium-enriched bacterial solution in step (2) comprises lysine bacillus and thermophilic streptococcus;
[0014] The concentration of Bacillus lysine hammer in the selenium-enriched bacterial solution is 1-2×10 10 Pieces / mL;
[0015] The concentration of thermophilic streptococci in the selenium-enriched bacterial solution is 3.5-4.5×10 10 Pieces / mL.
[0016] Preferably, the mass ratio of the selenium-enriched bacterial solution to the soaked wheat in step (2) is 0.5-0.9:90-110.
[0017] Preferably, the co-cultivation method in step (3) is: culturing the mixture at 35-38° C. for 2-4 days, then spreading the mixture to a thickness of 0.5-1.5 cm, and continuing to cultivate at 28-33° C. until the malt length reaches 1.5-3 cm;
[0018] During the co-cultivation process, the mixed material is supplemented with a sodium selenite aqueous solution to ensure that the water content of the mixed material is not less than 70%, and the concentration of sodium selenite in the sodium selenite aqueous solution is 280-320 mg / L.
[0019] Preferably, the temperature of the heat treatment in step (3) is 60-70° C., and the time of the heat treatment is 40-60 min.
[0020] Preferably, the washing in step (4) is carried out with clean water, the washing is performed 2 to 3 times, the roasting temperature is 120 to 140° C., and the moisture content of the roasted malt is 8 to 12%.
[0021] The invention also provides selenium-enriched malt powder prepared by the preparation method.
[0022] The invention also provides application of the selenium-enriched malt powder in preparing anti-tumor products.
[0023] The invention also provides application of the selenium-enriched malt powder in preparing a product for eliminating nodules.
[0024] The invention provides a selenium-enriched malt powder and a preparation method thereof, comprising the following steps: (1) soaking wheat and draining it to obtain soaked wheat; (2) mixing the soaked wheat and selenium-enriched bacterial solution to obtain a mixture; (3) co-culturing the mixture and then heat-treating it to obtain malt; (4) washing the malt, roasting it after washing, and crushing it to obtain selenium-enriched malt powder. The selenium-enriched malt powder of the invention can use existing wheat as a raw material, and there is no need to supplement selenium in the wheat growth stage. By introducing selenium-enriched bacteria and transformation bacteria to coordinate with wheat during the preparation process, selenium is enriched, and inorganic selenium is converted into organic selenium, thereby increasing the organic selenium content in the product. The preparation process is simple and reliable, and is suitable for large-scale promotion and application. The prepared selenium-enriched malt powder has the effects of anti-tumor and eliminating nodules, provides raw materials for the development of functional foods, and improves the nutritional and health value of products. DETAILED DESCRIPTION
[0025] The present invention provides a method for preparing selenium-enriched malt powder, comprising the following steps:
[0026] (1) soaking the wheat and then draining it to obtain soaked wheat;
[0027] (2) mixing the soaked wheat and the selenium-enriched bacterial solution to obtain a mixture;
[0028] (3) heat treating the mixed material after co-culturing to obtain malt;
[0029] (4) The malt is cleaned, roasted, and crushed to obtain selenium-enriched malt powder.
[0030] In the present invention, the wheat is preferably soaked in a sodium selenite aqueous solution in step (1), the mass ratio of the wheat to the sodium selenite aqueous solution is preferably 1:6-8, more preferably 1:7, the concentration of sodium selenite in the sodium selenite aqueous solution is preferably 280-320 mg / L, more preferably 300 mg / L, and the soaking time is preferably 2-6 h, more preferably 4 h.
[0031] In the present invention, the selenium-enriched bacterial solution in step (2) preferably includes lysine bacillus and thermophilic streptococcus;
[0032] The concentration of Bacillus hammer-shaped lysine in the selenium-enriched bacterial solution is preferably 1 to 2×10 10 / mL, more preferably 1.5×10 10 Pieces / mL;
[0033] The concentration of thermophilic streptococci in the selenium-enriched bacterial solution is preferably 3.5 to 4.5×10 10 / mL, more preferably 4×10 10 Pieces / mL.
[0034] In the present invention, the mass ratio of the selenium-enriched bacterial liquid to the soaked wheat in step (2) is preferably 0.5-0.9:90-110, and more preferably 0.7:100.
[0035] In the present invention, the co-cultivation method in step (3) is preferably: culturing the mixture at 35-38°C for 2-4 days, then spreading the mixture to a thickness of 0.5-1.5 cm, and continuing to cultivate at 28-33°C until the malt length reaches 1.5-3 cm, and more preferably: culturing the mixture at 36-37°C for 3 days, then spreading the mixture to a thickness of 1 cm, and continuing to cultivate at 30-31°C until the malt length reaches 2 cm;
[0036] During the co-cultivation process, the mixture is supplemented with a sodium selenite aqueous solution to ensure that the water content of the mixture is preferably not less than 70%. The concentration of sodium selenite in the sodium selenite aqueous solution is preferably 280-320 mg / L, and more preferably 300 mg / L.
[0037] In the present invention, the temperature of the heat treatment in step (3) is preferably 60-70°C, more preferably 65°C, and the time of the heat treatment is preferably 40-60 min, more preferably 50 min.
[0038] In the present invention, the washing in step (4) is preferably carried out with clean water, the washing is preferably carried out 2 to 3 times, the roasting temperature is preferably 120 to 140°C, more preferably 130°C, and the water content of the roasted malt is preferably 8 to 12%, more preferably 10%.
[0039] The invention also provides selenium-enriched malt powder prepared by the preparation method.
[0040] The invention also provides application of the selenium-enriched malt powder in preparing anti-tumor products.
[0041] The invention also provides application of the selenium-enriched malt powder in preparing a product for eliminating nodules.
[0042] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.
[0043] Example 1
[0044] A method for preparing selenium-enriched malt powder comprises the following steps:
[0045] (1) Prepare a sodium selenite aqueous solution with a concentration of 280 mg / L for standby use;
[0046] (2) mixing the wheat with an aqueous solution of sodium selenite 8 times its mass, soaking the mixture for 2 hours and then draining the mixture to obtain the soaked wheat;
[0047] (3) mixing 110 parts of the soaked wheat and 0.5 parts of the selenium-enriched bacterial solution in a mass ratio to obtain a mixture;
[0048] (4) culturing the mixture at 38° C. for 2 days, then spreading the mixture to a thickness of 0.5 cm, and continuing to culture at 33° C. until the malt length reaches 1.5 cm, supplementing the mixture with a sodium selenite aqueous solution during the culture process to ensure that the water content of the mixture is not less than 70%, and then performing a heat treatment at 60° C. for 60 min to obtain malt;
[0049] (5) Wash the malt twice with clean water, roast it at 120° C. until the moisture content of the malt reaches 12%, and grind it to obtain selenium-enriched malt powder.
[0050] Note: The selenium-rich bacterial solution in this embodiment includes lysine Bacillus fusiformis and thermophilic Streptococcus;
[0051] The concentration of Bacillus lysine hammer in the selenium-enriched bacterial solution is 2×10 10 Pieces / mL;
[0052] The concentration of thermophilic streptococci in the selenium-enriched bacterial solution is 3.5×10 10 Pieces / mL.
[0053] Example 2
[0054] A method for preparing selenium-enriched malt powder comprises the following steps:
[0055] (1) Prepare a sodium selenite aqueous solution with a concentration of 320 mg / L for standby use;
[0056] (2) mixing the wheat with a sodium selenite aqueous solution of 6 times its mass, soaking the mixture for 6 hours and then draining the mixture to obtain the soaked wheat;
[0057] (3) mixing 90 parts of the soaked wheat and 0.9 parts of the selenium-enriched bacterial solution in a mass ratio to obtain a mixture;
[0058] (4) culturing the mixture at 35° C. for 4 days, then spreading the mixture to a thickness of 1.5 cm, and continuing to cultivate at 28° C. until the malt length reaches 3 cm, supplementing the mixture with a sodium selenite aqueous solution during the cultivation process to ensure that the water content of the mixture is not less than 70%, and then heat treating the mixture at 70° C. for 40 minutes to obtain malt;
[0059] (5) Wash the malt with clean water for three times, roast it at 140° C. until the water content of the malt reaches 8%, and grind it to obtain selenium-enriched malt powder.
[0060] Note: The selenium-rich bacterial solution in this embodiment includes lysine Bacillus fusiformis and thermophilic Streptococcus;
[0061] The concentration of Bacillus lysine hammer in the selenium-enriched bacterial solution is 1×10 10 Pieces / mL;
[0062] The concentration of thermophilic streptococci in the selenium-enriched bacterial solution is 4.5×10 10 Pieces / mL.
[0063] Example 3
[0064] A method for preparing selenium-enriched malt powder comprises the following steps:
[0065] (1) Prepare a 300 mg / L sodium selenite aqueous solution for standby use;
[0066] (2) mixing the wheat with an aqueous solution of sodium selenite having a mass of 7 times the mass thereof, soaking the mixture for 4 hours and then draining the mixture to obtain the soaked wheat;
[0067] (3) mixing 100 parts of the soaked wheat and 0.7 parts of the selenium-enriched bacterial solution in a mass ratio to obtain a mixture;
[0068] (4) culturing the mixture at 37° C. for 3 days, then spreading the mixture to a thickness of 1 cm, and continuing to culture at 30° C. until the malt length reaches 2 cm, supplementing the mixture with a sodium selenite aqueous solution during the culture process to ensure that the water content of the mixture is not less than 70%, and then heat treating the mixture at 65° C. for 50 min to obtain malt;
[0069] (5) Wash the malt with clean water for three times, roast it at 130° C. until the water content of the malt reaches 10%, and grind it to obtain selenium-enriched malt powder.
[0070] Note: The selenium-rich bacterial solution in this embodiment includes lysine Bacillus fusiformis and thermophilic Streptococcus;
[0071] The concentration of Bacillus lysine hammer in the selenium-enriched bacterial solution is 1.5×10 10 Pieces / mL;
[0072] The concentration of thermophilic streptococci in the selenium-enriched bacterial solution is 4×10 10 Pieces / mL.
[0073] Comparative Example 1
[0074] The difference from Example 3 is that the thermophilic Streptococcus was discarded and the concentration of Bacillus lysine hammer was increased to 5.5×10 10 Pieces / mL.
[0075] Comparative Example 2
[0076] The difference from Example 3 is that the hammer-shaped lysine Bacillus was discarded and the concentration of thermophilic Streptococcus was increased to 5.5×10 10 Pieces / mL.
[0077] Test example
[0078] 1. Selenium content test
[0079] The selenium content in the selenium-rich malt powder prepared in Examples 1 to 3 and Comparative Examples 1 to 2 was tested. The detection method referred to the detection method for the total selenium content of the product in GB 5009.932017 "Determination of Selenium in Foods". However, since this method detects all the selenium content in the selenium-rich malt powder, it is impossible to distinguish between organic selenium and inorganic selenium. Therefore, after measuring the total selenium content in the selenium-rich malt powder, the same selenium-rich malt powder is extracted with ethanol, and the organic selenium in the malt powder is extracted into ethanol. The content is determined again by the method in GB5009.932017 "Determination of Selenium in Foods", and the proportion of organic selenium in the same malt powder can be obtained.
[0080] Experimental results: as shown in Table 1.
[0081] Table 1
[0082] Group Total selenium content mg / kg Organic selenium content mg / kg Organic selenium content% Example 1 518 491 94.97 Example 2 521 489 93.86 Example 3 531 501 94.35 Comparative Example 1 441 199 45.12 Comparative Example 2 335 297 88.66
[0083] 2. Safety test
[0084] 60 SD clean-grade male rats weighing 180-220 g were purchased from Shanghai Slake Experimental Animal Co., Ltd. The rats were housed in separate cages. Adaptive feeding was performed for 3 days before the start of the experiment and the rats were randomly divided into 6 groups, 10 rats in each group, numbered as experimental groups 1-3, control groups 1-2 and blank control group.
[0085] After the start of the experiment, the rats in each group were fed separately. The rats in the experimental group 1 were fed the selenium-enriched malt powder prepared in Example 1 at a standard of 0.8 g / day / rat, and the other groups were fed according to the same standard, wherein the experimental group 2 was fed the selenium-enriched malt powder prepared in Example 2, the experimental group 3 was fed the selenium-enriched malt powder prepared in Example 3, the control group 1 was fed the selenium-enriched malt powder prepared in Comparative Example 1, the control group 2 was fed the selenium-enriched malt powder prepared in Comparative Example 2, and the blank control group was not fed any selenium-enriched malt powder.
[0086] During the experiment, the rats were allowed to eat, drink and move freely for 21 consecutive days. The health status of rats in each group was observed after the experiment.
[0087] result:
[0088] The test results showed that there was no significant difference between the rats in the test groups 1 to 3 and the rats in the control groups 1 to 2 and the rats in the blank control group, and all of them became sick or died, indicating that the product prepared by the present invention is relatively safe.
[0089] 3. Use effect test
[0090] Fifty SD clean-grade male rats weighing 180-220 g were purchased from Shanghai Slake Experimental Animal Co., Ltd. The rats were housed in separate cages. Adaptive feeding was performed for 3 days before the start of the experiment and the rats were randomly divided into 5 groups, 10 rats in each group, numbered as experimental groups 4-6, control group 3 and blank control group.
[0091] Liver cancer models were established for rats in test groups 4 to 6 and control group 3, and no model was established for rats in the blank control group. After the model was established, the rats in Example 4 were fed with a feed containing the selenium-enriched malt powder prepared in Example 1, wherein the selenium content in the feed was 3.0 mg / kg.
[0092] The other groups were fed according to the same standard, wherein the experimental group 5 was fed with the selenium-enriched malt powder prepared in Example 2, the experimental group 6 was fed with the selenium-enriched malt powder prepared in Example 3, and the rats in the blank control group and the control group 5 were fed with ordinary feed without any selenium-enriched malt powder.
[0093] The modeling process was based on the method in the literature (Zou Jing, Chen Jianjie. Preventive effect of Bushen prescription on diethylnitrosamine-induced liver cancer in rats [J]. Journal of Integrated Traditional Chinese and Western Medicine for Hepatology 2003, 13(5): 278-280). The modeling was carried out by drinking a 100 mg / L DEN solution freshly prepared with sterile tap water to maintain a DEN intake of about 10 mg / kg per day for 16 consecutive weeks, and then drinking sterile tap water until the end of the experiment at the end of the 18th week. At the end of the 18th week, blood was collected from the orbits of the rats in each group, and the plasma was separated and frozen at -20°C with heparin anticoagulation for biochemical index analysis.
[0094] Number of liver surface cancer nodules: Counted according to the literature (Mereno FS, Rizzi M BSL, Dagli MLZ, et al. Inhibitory effect of B-corotence on preneoplastic lesions induced in wister rats by theresitent hepatocyte model [J]. Carcinogenesis. 1991, 12: 1817-1822). Plasma alanine aminotransferase (ALT), alkaline phosphatase (ALP), albumin (ALB), and total bilirubin (TBIL) were measured using an automatic biochemical analyzer; GST and γ-GT were measured using a colorimetric method according to the instructions of the kit.
[0095] Experimental results: as shown in Table 2.
[0096] Table 2
[0097]
[0098]
[0099] As can be seen from Table 2, compared with the rats in the control group 3, the number of liver surface cancer nodules, ALT, ALP, ALB, TBIL, GST and γ-GT in the rats of the test groups 3 to 5 were significantly reduced, indicating that the selenium-enriched malt powder prepared by the present invention has the effects of reducing liver damage, delaying the formation of liver cancer in rats, and inhibiting the formation of liver cancer nodules.
[0100] As can be seen from the above embodiments, the present invention provides a selenium-enriched malt powder and a preparation method thereof, comprising the following steps: (1) soaking wheat and draining to obtain soaked wheat; (2) mixing the soaked wheat and selenium-enriched bacterial solution to obtain a mixture; (3) co-culturing the mixture and heat-treating it to obtain malt; (4) washing the malt, roasting it after washing, and crushing it to obtain selenium-enriched malt powder. The selenium-enriched malt powder of the present invention can use existing wheat as a raw material, and there is no need to supplement selenium during the wheat growth stage. By introducing selenium-enriched bacteria and transformation bacteria to coordinate with wheat during the preparation process, selenium is enriched, and inorganic selenium is converted into organic selenium, thereby increasing the organic selenium content in the product. The preparation process is simple and reliable, and is suitable for large-scale promotion and application. The prepared selenium-enriched malt powder has the effects of anti-tumor and eliminating nodules, provides raw materials for the development of functional foods, and improves the nutritional and health value of products.
[0101] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.
Claims
1. A method for preparing selenium-enriched malt powder, characterized in that: The steps include: (1) soaking the wheat and then draining it to obtain soaked wheat; (2) mixing the soaked wheat and the selenium-enriched bacterial solution to obtain a mixture; (3) heat treating the mixed material after co-culturing to obtain malt; (4) The malt is cleaned, roasted, and crushed to obtain selenium-enriched malt powder.
2. The preparation method according to claim 1, characterized in that: The wheat is soaked in a sodium selenite aqueous solution in step (1), the mass ratio of the wheat to the sodium selenite aqueous solution is 1:6-8, the concentration of sodium selenite in the sodium selenite aqueous solution is 280-320 mg / L, and the soaking time is 2-6 hours.
3. The preparation method according to claim 2, characterized in that: The selenium-enriched bacterial solution in step (2) includes lysine bacillus and thermophilic streptococcus; The concentration of Bacillus lysine hammer in the selenium-enriched bacterial solution is 1-2×10 10 Pieces / mL; The concentration of thermophilic streptococci in the selenium-enriched bacterial solution is 3.5-4.5×10 10 Pieces / mL.
4. The preparation method according to claim 3, characterized in that: The mass ratio of the selenium-enriched bacterial solution to the soaked wheat in step (2) is 0.5-0.9:90-110.
5. The preparation method according to claim 4, characterized in that: The co-cultivation method in step (3) is: culturing the mixture at 35-38° C. for 2-4 days, then spreading the mixture to a thickness of 0.5-1.5 cm, and continuing to cultivate at 28-33° C. until the malt length reaches 1.5-3 cm; During the co-cultivation process, the mixed material is supplemented with a sodium selenite aqueous solution to ensure that the water content of the mixed material is not less than 70%, and the concentration of sodium selenite in the sodium selenite aqueous solution is 280-320 mg / L.
6. The preparation method according to claim 5, characterized in that: The temperature of the heat treatment in step (3) is 60-70° C., and the time of the heat treatment is 40-60 min.
7. The preparation method according to any one of claims 1 to 6, characterized in that: The washing in step (4) is carried out with clean water, the washing is performed 2 to 3 times, the roasting temperature is 120 to 140° C., and the water content of the roasted malt is 8 to 12%.
8. Selenium-enriched malt powder prepared by the preparation method according to any one of claims 1 to 7.
9. Use of the selenium-enriched malt powder according to claim 8 in the preparation of anti-tumor products.
10. Use of the selenium-enriched malt powder according to claim 8 in preparing a product for eliminating nodules.