Animal ovum negative pressure suction instrument

Abstract

The invention relates to the technical field of animal breeding, and aims to provide an animal ovum negative pressure suction instrument which comprises a software and hardware control module, a negative pressure generation module, an oocyte suction device, a heating test tube rack and an operation display module. According to the invention, the intelligent processor is adopted to control the temperature setting and working parameters and the negative pressure setting and working parameters, so that the control precision and the working efficiency are relatively high; the liquid crystal screen accurately displays set and real-time working temperature and negative pressure values, full Chinese display is achieved, data are visual and clear, user operation is easy and convenient, and the product can be widely applied to actual requirements of animal embryo breeding, cell operation processing, animal scientific research experiments and the like.

Description

Technical Field

[0001] The invention relates to the technical field of animal reproduction, in particular to an animal egg negative pressure suction instrument. Background Art

[0002] Artificial animal breeding can rapidly breed animals with excellent traits and economic value, and can also effectively protect rare animals. Furthermore, in human biological research, some technologies involve ethical issues, so it is appropriate to indirectly study human physiological phenomena through animal experiments. Animal in vitro fertilization technology is not only used in large-scale livestock production, but also becomes an important auxiliary means for studying other embryonic biotechnologies, such as cloning, transgenics, embryonic stem cell isolation and culture, and sex control.

[0003] Ultrasonic detection-assisted negative pressure suction is often used for live egg retrieval in cattle, horses and other large livestock. The method is to use B-type ultrasound image guidance to insert the egg retrieval needle through the vaginal wall and use negative pressure to absorb the oocytes in large follicles. According to the current technical level, a healthy cow can obtain 5 to 10 eggs per week. The oocytes collected from live bodies are matured and cultured and then fertilized with sperm. This method has significant social and economic benefits for expanding excellent breeds and increasing production. In recent years, commercial production has developed rapidly at home and abroad.

[0004] In the process of live animal egg retrieval and in vitro fertilization, the negative pressure oocyte extractor is a key device. At present, most relevant users use the negative pressure oocyte extractor of Minitu Company in Germany. This equipment occupies most of the international and domestic markets due to its early development and relative maturity. However, the main problem of the existing equipment is that it uses mechanical devices to achieve negative pressure control, which is costly and expensive. At the same time, the negative pressure and temperature control are unstable, and the absorption of bovine oocytes is prone to failure, which reduces the qualified rate. According to the survey, most domestic and foreign users hope to provide similar products with stable and accurate negative pressure and temperature control, and can reduce the price and use cost of the equipment. Therefore, the localization of animal egg negative pressure extractors controlled by software and hardware is of great significance. Summary of the invention

[0005] The purpose of the present invention is to provide an animal egg negative pressure suction instrument to solve the problems raised in the background technology and facilitate its promotion.

[0006] An animal egg negative pressure suction instrument, comprising a software and hardware control module, a negative pressure generating module, an oocyte suction device, a heating test tube rack, and an operation display module;

[0007] The oocyte suction device includes an egg retrieval needle, one end of the egg retrieval needle is provided with a hose connector, the hose connector is connected to a silicone plug, the end of the silicone plug away from the hose connector is provided with a thin vacuum tube, the end of the hose connector away from the egg retrieval needle is provided with an extraction connecting tube, the end of the extraction connecting tube away from the hose connector is provided with a pipeline connector, the pipeline connector is inserted on the tube body, a thick vacuum tube is provided on the pipeline connector, the end of the thick vacuum tube away from the pipeline connector is provided with a pneumatic joint, an oocyte collection tube is connected to the pneumatic joint, and the oocyte collection tube is provided with a collection connector at one end away from the pneumatic joint.

[0008] Furthermore, the software and hardware control module includes a control board U1, an air pressure sensor PD, a temperature transmitter AD, a first signal driving circuit, a second signal driving circuit and a solenoid valve R, wherein the first signal driving circuit includes a first field effect transistor MOS1 and a first rectifier diode D1, and the second signal driving circuit includes a second field effect transistor MOS2 and a second rectifier diode D2. The analog input AO pin of the control board U1 is connected to the output signal of the air pressure sensor PD, the digital output pin of the control board U1 is connected to the first pin of the solenoid valve R, the second pin of the solenoid valve R is grounded, and the third pin of the solenoid valve R is connected to 24 The fourth pin of the solenoid valve R is connected to the gas valve Y of the solenoid valve R, the PWM2 pin of the control board U1 is connected to the gate of the first field effect tube MOS1, the drain of the first field effect tube MOS1 is connected to an input end of the negative pressure pump motor M of the negative pressure generating module and the anode of the first rectifier diode D1, the source of the first field effect tube MOS1 is grounded, the cathode of the first rectifier diode D1 is connected to the other input end of the negative pressure pump motor M and then connected to a 24 volt voltage, the output end of the negative pressure pump motor M is connected to the negative pressure pump air pump, the air pressure sensor PD transmits the measured air pressure value to the controller U1, and the controller U1 controls the opening and closing state of the air valve Y.

[0009] Furthermore, the negative pressure generating module comprises a negative pressure pump and an air storage tank, and the negative pressure pump comprises a motor and an air pump.

[0010] Furthermore, the operation display module includes a temperature increase setting button, a temperature decrease setting button, a negative pressure setting knob, an emergency start button, a wireless foot switch, a wireless receiver and a liquid crystal display screen.

[0011] Furthermore, the heated test tube rack is an aluminum alloy test tube rack with a heating plate.

[0012] Furthermore, the PWM1 pin of the control board U1 is connected to the gate of the second field effect transistor MOS2, the drain of the second field effect transistor MOS2 is connected to an input end of the electric heater S of the heating test tube rack and the anode of the second rectifier diode D2, the source of the second field effect transistor MOS2 is grounded, the cathode of the second rectifier diode D2 is connected to the other input end of the electric heater S of the heating test tube rack and then connected to a 24 volt voltage, the analog input A1 pin of the control board U1 is connected to the output end signal of the temperature transmitter AD, the temperature transmitter AD transmits the measured temperature value signal to the controller U1, and the controller U1 then controls the electric heater S of the heating test tube rack through the second signal driving circuit.

[0013] Furthermore, the six digital input pins of the control board U1 are respectively connected to the emergency start button K1, the negative pressure setting knob K2, the temperature increase setting button K3, the temperature decrease setting button K4, the wireless receiver K5, and the LCD display screen XSP of the operation display module.

[0014] As an improvement, the beneficial effects of the present invention are:

[0015] 1. Designed the hardware circuit of the negative pressure absorber, mainly including the temperature measurement circuit, air pressure signal acquisition circuit, filter circuit, drive control circuit, power supply circuit and microcontroller peripheral circuit. Completed the design, debugging and production of the circuit schematic and PCB board according to the hardware design requirements, achieving the stability and reliability of the product function.

[0016] 2. Developed a self-tuning control software algorithm for the constant temperature control parameters of egg retrieval and an adaptive fuzzy feedback control software program, so that the system can obtain more accurate temperature control performance.

[0017] By combining the temperature sensor AD feedback information and control algorithm design, the processor achieves precise temperature control of the test tube rack electric heating module, and the real-time temperature can be controlled within an error of ±0.5 degrees of the set temperature.

[0018] When the heating is about to reach the temperature stable state, in order to avoid the overshoot phenomenon caused by full-power heating and reduce the temperature rise rate, the system will automatically use the fuzzy PID controller parameter setting to control the heating of the test tube rack, and correct the control parameters in real time to play the role of heating buffer, thereby improving the stability and anti-interference ability of the temperature control system. Avoid the shortcomings of low temperature accuracy and easy overshoot of the heating test tube rack of foreign products.

[0019] 3. Developed a software algorithm for self-tuning air pressure control of egg retrieval negative pressure and suction parameters and an adaptive fuzzy control software program, so that the system design can obtain more accurate negative pressure control performance.

[0020] By combining the air pressure sensor PD feedback information and control algorithm, the controller U1 realizes precise control of the negative pressure of the negative pressure pump air circuit module, and the real-time negative pressure can be controlled within the error of the set negative pressure ±5MMHg.

[0021] The precise control of the damping of the negative pressure pump, especially because the damping control is achieved electronically, avoids the shortcomings of foreign products' mechanical devices that are prone to failure and have low pass rates. At the same time, the innovatively designed pneumatic adaptation control software for the negative pressure pump and air tank makes the air pressure rise relatively smooth and the negative pressure output stable and accurate, which improves the success rate and pass rate of oocyte absorption.

[0022] 4. The negative pressure flow direction controlled by the solenoid valve is accurately switched in real time through the processor software, thereby avoiding the possibility of oocyte fluid backflow, protecting the negative pressure generating system from reliable operation and preventing instrument damage and operation failure. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a structural schematic diagram of an oocyte suction device of an animal egg negative pressure suction instrument of the present invention;

[0024] Figure 2 This is a schematic diagram of the structure of the software and hardware control modules of an animal egg negative pressure suction instrument of the present invention;

[0025] Figure 3 This is a circuit diagram of a software and hardware control module of an animal egg negative pressure suction instrument of the present invention;

[0026] Reference table of reference numerals:

[0027] 1. Egg retrieval needle; 2. Hose connector; 3. Extraction connecting tube; 4. Silicone plug; 5. Thin vacuum tube; 6. Pipeline connector; 7. Thick vacuum tube; 8. Pneumatic connector; 9. Oocyte collection tube; 10. Collection connector; 11. Tube body. DETAILED DESCRIPTION

[0028] The specific embodiments of the present invention are further described below in conjunction with the accompanying drawings. The same components are represented by the same reference numerals. It should be noted that the words "front", "rear", "left", "right", "upper" and "lower" used in the following description refer to directions in the accompanying drawings, and the words "inner" and "outer" refer to directions toward or away from the geometric center of a specific component, respectively.

[0029] In order to make the content of the present invention more clearly understood, the technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings in the embodiments of the present invention, wherein the same components are represented by the same reference numerals.

[0030] According to this embodiment Figures 1 to 3As shown, an animal egg negative pressure suction instrument includes a software and hardware control module, a negative pressure generating module, an oocyte suction device, a heating test tube rack, and an operation display module.

[0031] In this embodiment, the oocyte suction device includes an egg retrieval needle 1, a hose connector 2 is provided at one end of the egg retrieval needle 1, a silicone plug 4 is connected to the hose connector 2, a thin vacuum tube 5 is provided at the end of the silicone plug 4 away from the hose connector 2, an extraction connecting tube 3 is provided at the end of the hose connector 2 away from the egg retrieval needle 1, a pipeline connector 6 is provided at the end of the extraction connecting tube 3 away from the hose connector 2, the pipeline connector 6 is inserted into the tube body 11, a thick vacuum tube 7 is provided on the pipeline connector 6, a pneumatic joint 8 is provided at the end of the thick vacuum tube 7 away from the pipeline connector 6, an oocyte collection tube 9 is connected to the pneumatic joint 8, and a collection connector 10 is provided at the end of the oocyte collection tube 9 away from the pneumatic joint 8.

[0032] In this embodiment, the software and hardware control module includes a control board U1, an air pressure sensor PD, a temperature transmitter AD, a first signal driving circuit, a second signal driving circuit and an electromagnetic valve R. The first signal driving circuit includes a first field effect transistor MOS1 and a first rectifier diode D1, and the second signal driving circuit includes a second field effect transistor MOS2 and a second rectifier diode D2. The analog input AO pin of the control board U1 is connected to the output signal of the air pressure sensor PD, the digital output pin of the control board U1 is connected to the first pin of the electromagnetic valve R, the second pin of the electromagnetic valve R is grounded, and the third pin of the electromagnetic valve R is connected to a 24 volt voltage. The fourth pin of the solenoid valve R is connected to the air valve Y of the solenoid valve R, the PWM2 pin of the control board U1 is connected to the gate of the first field effect transistor MOS1, the drain of the first field effect transistor MOS1 is connected to an input end of the negative pressure pump motor M of the negative pressure generating module and the anode of the first rectifier diode D1, the source of the first field effect transistor MOS1 is grounded, the cathode of the first rectifier diode D1 is connected to the other input end of the negative pressure pump motor M and then to a 24 volt voltage, the output end of the negative pressure pump motor M is connected to the negative pressure pump air pump, the air pressure sensor PD transmits the measured air pressure value to the controller U1, and the controller U1 controls the opening and closing state of the air valve Y.

[0033] In this embodiment, the negative pressure generating module includes a negative pressure pump and an air storage tank, and the negative pressure pump includes a motor and an air pump.

[0034] In this embodiment, the operation display module includes a temperature increase setting button, a temperature decrease setting button, a negative pressure setting knob, an emergency start button, a wireless foot switch, a wireless receiver and a liquid crystal display screen.

[0035] In this embodiment, the heated test tube rack is an aluminum alloy test tube rack with a heating plate.

[0036] In this embodiment, the PWM1 pin of the control board U1 is connected to the gate of the second field effect transistor MOS2, the drain of the second field effect transistor MOS2 is connected to an input end of the electric heater S of the heating test tube rack and the anode of the second rectifier diode D2, the source of the second field effect transistor MOS2 is grounded, the cathode of the second rectifier diode D2 is connected to the other input end of the electric heater S of the heating test tube rack and then connected to a 24 volt voltage, the analog input A1 pin of the control board U1 is connected to the output end signal of the temperature transmitter AD, the temperature transmitter AD transmits the measured temperature value signal to the controller U1, and the controller U1 then controls the electric heater S of the heating test tube rack through the second signal driving circuit.

[0037] In this embodiment, the six digital input pins of the control board U1 are respectively connected to the emergency start button K1, the negative pressure setting knob K2, the temperature increase setting button K3, the temperature decrease setting button K4, the wireless receiver K5, and the LCD display screen XSP of the operation display module.

[0038] The working steps of this embodiment are as follows:

[0039] S1. Turn on the power, start the temperature setting operation, adjust the temperature increase setting button K3 or the temperature decrease setting button K4, set the appropriate test tube rack temperature, and the test tube rack heating enters the working state.

[0040] S2. According to actual operation requirements, adjust the negative pressure setting knob K2 to set an appropriate negative pressure setting value.

[0041] S3. After the test tube rack is heated, when the actual temperature and the set temperature are less than ±0.5℃ and stabilized for ten minutes, you can step on the wireless foot switch (short time) or press the emergency start button K1 (short time), and the negative pressure generating module starts to run. Observe that the real-time negative pressure value displayed on the LCD screen XSP and the set negative pressure value are stably less than ±5mmHg. After stabilization, start the egg retrieval operation.

[0042] S4. If the pipeline is blocked and the maximum negative pressure is required, press and hold the emergency start button or the wireless foot switch (for more than 3 seconds) and the negative pressure pump will generate a maximum suction force of 480 mmHg to relieve the blockage of the pipeline by the oocyte liquid tissue.

[0043] S5. After the operation is completed, step on the wireless foot switch (short time) or press the emergency start button K1 (short time), the negative pressure generating module stops working, then check the instrument status is normal, and then turn off the power switch on the back of the instrument.

[0044] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. An animal egg negative pressure suction instrument, comprising a software and hardware control module, a negative pressure generating module, an oocyte suction device, a heating test tube rack, and an operation display module; The oocyte suction device comprises an egg retrieval needle (1), one end of the egg retrieval needle (1) is provided with a hose connector (2), the hose connector (2) is connected with a silicone plug (4), the end of the silicone plug (4) away from the hose connector (2) is provided with a thin vacuum tube (5), the end of the hose connector (2) away from the egg retrieval needle (1) is provided with an extraction connection tube (3), the end of the extraction connection tube (3) away from the hose connector (2) is provided with a pipeline connector (6), the pipeline connector (6) is inserted into a tube body (11), a thick vacuum tube (7) is provided on the pipeline connector (6), the end of the thick vacuum tube (7) away from the pipeline connector (6) is provided with a pneumatic joint (8), the pneumatic joint (8) is connected with an oocyte collection tube (9), and the end of the oocyte collection tube (9) away from the pneumatic joint (8) is provided with a collection connector (10).

2. The animal egg negative pressure suction instrument according to claim 1, characterized in that: The software and hardware control module includes a control board U1, an air pressure sensor PD, a temperature transmitter AD, a first signal driving circuit, a second signal driving circuit and an electromagnetic valve R. The first signal driving circuit includes a first field effect transistor MOS1 and a first rectifier diode D1. The second signal driving circuit includes a second field effect transistor MOS2 and a second rectifier diode D2. The analog input AO pin of the control board U1 is connected to the output signal of the air pressure sensor PD, the digital output pin of the control board U1 is connected to the first pin of the electromagnetic valve R, the second pin of the electromagnetic valve R is grounded, and the third pin of the electromagnetic valve R is connected to a 24 volt voltage. The fourth pin of the solenoid valve R is connected to the air valve Y of the solenoid valve R, the PWM2 pin of the control board U1 is connected to the gate of the first field effect tube MOS1, the drain of the first field effect tube MOS1 is connected to an input end of the negative pressure pump motor M of the negative pressure generating module and the anode of the first rectifier diode D1, the source of the first field effect tube MOS1 is grounded, the cathode of the first rectifier diode D1 is connected to the other input end of the negative pressure pump motor M and then connected to a 24 volt voltage, the output end of the negative pressure pump motor M is connected to the negative pressure pump air pump, the air pressure sensor PD transmits the measured air pressure value to the controller U1, and the controller U1 controls the opening and closing state of the air valve Y.

3. The animal egg negative pressure suction instrument according to claim 2, characterized in that: The negative pressure generating module comprises a negative pressure pump and an air storage tank, wherein the negative pressure pump comprises a motor and an air pump.

4. The animal egg negative pressure suction instrument according to claim 3, characterized in that: The operation display module includes a temperature increase setting button, a temperature decrease setting button, a negative pressure setting knob, an emergency start button, a wireless foot switch, a wireless receiver and a liquid crystal display screen.

5. The animal egg negative pressure suction instrument according to claim 4, characterized in that: The heated test tube rack is an aluminum alloy test tube rack with a heating plate.

6. An animal egg negative pressure suction instrument according to any one of claims 2 or 5, characterized in that: The PWM1 pin of the control board U1 is connected to the gate of the second field effect tube MOS2, the drain of the second field effect tube MOS2 is connected to an input end of the electric heater S of the heating test tube rack and the anode of the second rectifier diode D2, the source of the second field effect tube MOS2 is grounded, the cathode of the second rectifier diode D2 is connected to the other input end of the electric heater S of the heating test tube rack and then connected to a 24 volt voltage, the analog input A1 pin of the control board U1 is connected to the output end signal of the temperature transmitter AD, the temperature transmitter AD transmits the measured temperature value signal to the controller U1, and the controller U1 controls the electric heater S of the heating test tube rack through the second signal driving circuit.

7. An animal egg negative pressure suction instrument according to any one of claims 2 or 4, characterized in that: The six digital input pins of the control panel U1 are respectively connected to the emergency start button K1, the negative pressure setting knob K2, the temperature increase setting button K3, the temperature decrease setting button K4, the wireless receiver K5, and the liquid crystal display screen XSP of the operation display module.

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