Repair composition containing PN and sodium hyaluronate as well as preparation method and application of repair composition

Abstract

The invention provides a repairing composition containing PN and sodium hyaluronate as well as a preparation method and application of the repairing composition, and relates to the technical fields of medicines, medical instruments and cosmetics. The repairing composition is prepared from the following components in parts by weight: 1 to 4 parts of PN, 30 to 60 parts of sodium hyaluronate, 3 to 8 parts of palmitoyl tripeptide-5, 2.5 to 5 parts of radix notoginseng extract, 0.5 to 2 parts of radix astragali seu hedysari extract, 0.5 to 2 parts of recombinant human epidermal growth factor and 0.5 to 2 parts of calendula extract. The pseudo-ginseng extract is subjected to heating treatment by adopting an alkaline substance, so that dissolution of active ingredients is facilitated; the astragalus extract is subjected to decoction and microwave-assisted treatment, so that the content of astragalus polysaccharide and other components is increased; all the components interact with one another, so that the effects of remarkably promoting wound healing, resisting inflammation and improving skin acne pigmentation are achieved.

Description

Technical Field

[0001] The present invention belongs to the technical field of medicine, medical equipment and cosmetics, and specifically relates to a repair composition containing PN and sodium hyaluronate, and a preparation method and application thereof. Background Art

[0002] PN (polynucleotide) has a three-dimensional scaffold structure of long-chain double helix, which can build space for extracellular matrix growth, help promote the ability of water condensation in the skin, thereby improving the skin's ability to lock in moisture, improve skin texture, smooth fine lines, and achieve anti-aging and skin repair effects.

[0003] Sodium hyaluronate is a type of glucuronic acid that can promote the proliferation and differentiation of epidermal cells and help repair damaged skin tissue. It can also remove oxygen free radicals, promote the regeneration of skin in injured areas, reduce UV damage to the skin, and repair sunburned skin. In addition, sodium hyaluronate has a certain repair effect on skin damage, can promote skin cell growth and wound healing, restore damaged areas to health, and reduce scar formation.

[0004] The skin is the largest immune barrier of the human body, which can prevent the invasion of microorganisms and harmful substances and maintain the stability of the human body's internal environment. Wounds can damage the integrity and continuity of human skin. Related studies have shown that the migration speed of wound epidermal cells is faster in a moist healing environment than in an exposed wound environment, which can quickly repair the skin. Since sodium hyaluronate can attract water, polysaccharide molecules form a colloidal solution in water and provide a moist environment, sodium hyaluronate is widely used in the preparation of products that promote wound healing and repair skin.

[0005] Chinese invention patent CN117598929A discloses a skin care composition for dressing, its preparation method and use, the composition includes components: hyaluronic acid or its salt, ectoine or its derivatives and polydeoxyribonucleotides, the mass ratio of hyaluronic acid or its salt, ectoine and polydeoxyribonucleotides is 1:0.5-30:0.033, and the molecular weight of hyaluronic acid or its salt is 200KDa-1500KDa. The composition can reduce skin texture, refine pores, and improve skin fineness. At the same time, it can also effectively hinder pigmentation and deposition, and has a certain repair effect on mild to moderate acne damaged skin and superficial scars. However, the dressing prepared by the composition has a high hardness, poor skin feel after long-term application, and is not conducive to the repair of damaged skin.

[0006] Chinese invention patent CN116687809A discloses an anti-aging beauty skin care product composition containing nicotinamide mononucleotide, comprising ingredients: nicotinamide mononucleotide, Polygonum cuspidatum extract, icariin, ginseng extract, Astragalus extract, Evodia rutaecarpa, resveratrol, Rhus verniciflua flavonoids, Butea superba, oligomeric sodium hyaluronate and hydroxyethyl cellulose. This combination can delay aging, but has limited effect on skin repair. Chinese invention patent CN113520991A discloses a nanobiogel for blocking scar formation and reverse repair regulation and a preparation method thereof, wherein the nanobiogel for blocking scar formation and reverse repair regulation is composed of yeast-β-glucan, recombinant human epidermal growth factor, targeted cell receptor activator, sodium hyaluronate, glycerol glucoside, vitamin A, vitamin E, lysozyme, chitosan quaternary ammonium salt, sodium L-pyrrolidonecarboxylate, allantoin, Chinese medicine extract, plant essential oil and water, and has a significant repair effect on various types of scars and scars.

[0007] Although existing compositions containing sodium hyaluronate have a certain repairing effect on the skin, the efficacy of most products is limited to the effect of the active ingredients themselves, and does not reflect the synergistic effect between the ingredients. Moreover, some products contain multiple ingredients, which can easily aggravate the damage of sensitive skin. Summary of the invention

[0008] In view of the problems existing in the prior art, the present invention provides a repair composition containing PN and sodium hyaluronate, and a preparation method and application thereof. The components of the repair composition include: PN, sodium hyaluronate, palmitoyl tripeptide-5, Panax notoginseng extract, Astragalus extract, recombinant human epidermal growth factor and calendula extract; palmitoyl tripeptide-5, recombinant human epidermal growth factor and three kinds of extract components interact with each other to bring about the effects of synergistically promoting wound healing and anti-inflammatory; Panax notoginseng extract and Astragalus extract are prepared respectively by specific methods; the repair composition of the present invention has no irritation to the skin and brings about significant anti-oxidation and skin repair effects.

[0009] To achieve the above purpose, the technical solution adopted by the present invention is as follows: First, the present invention provides a repair composition containing PN and sodium hyaluronate, and the components in parts by weight include: 1-4 parts of PN, 30-60 parts of sodium hyaluronate, 3-8 parts of palmitoyl tripeptide-5, 2.5-5 parts of Panax notoginseng extract, 0.5-2 parts of Astragalus extract, 0.5-2 parts of recombinant human epidermal growth factor and 0.5-2 parts of calendula extract.

[0010] Preferably, the repair composition comprises, by weight, 2-3 parts of PN, 35-50 parts of sodium hyaluronate, 5-7 parts of palmitoyl tripeptide-5, 3-4 parts of Panax notoginseng extract, 1-1.5 parts of Astragalus extract, 1-1.5 parts of recombinant human epidermal growth factor and 1-1.5 parts of calendula extract.

[0011] Further preferably, the repair composition comprises, by weight, 2.5 parts of PN, 45 parts of sodium hyaluronate, 6 parts of palmitoyl tripeptide-5, 3.5 parts of Panax notoginseng extract, 1.2 parts of Astragalus extract, 1.2 parts of recombinant human epidermal growth factor and 1.3 parts of calendula extract.

[0012] Preferably, the weight ratio of PN, sodium hyaluronate, palmitic acid tripeptide-5 and recombinant human epidermal factor is 2-3:35-50:5-7:1-1.5.

[0013] Further preferably, the weight ratio of PN, sodium hyaluronate, palmitic acid tripeptide-5 and recombinant human epidermal factor is 2.5:45:6:1.2.

[0014] Preferably, the molecular weight of the sodium hyaluronate is 120 kDa-200 kDa.

[0015] More preferably, the molecular weight of the sodium hyaluronate is 150 kDa-180 kDa.

[0016] More preferably, the molecular weight of the sodium hyaluronate is 160 kDa.

[0017] Then, the present invention provides a method for preparing the above-mentioned repair composition, comprising the steps of: (1) Preparation method of Panax notoginseng extract: Panax notoginseng and alkaline substances are subjected to heating treatment to obtain Panax notoginseng extract; (2) Preparation method of astragalus extract: Astragalus is decocted in water and then subjected to microwave treatment to obtain the astragalus extract; (3) Mixing the Panax notoginseng extract, the Astragalus extract and other components in the composition to obtain a repair composition.

[0018] Preferably, in step (1), the alkaline substance is selected from at least one of sodium carbonate and sodium bicarbonate.

[0019] Preferably, step (1) is specifically as follows: the Panax notoginseng is crushed, mixed with water at a solid-liquid ratio of 1:10-20 g / mL, the pH is adjusted to 9-12 using an alkaline substance, heated under reflux extraction at 75-95° C. for 4-6 h, filtered, and the filtrate is concentrated and dried to obtain the Panax notoginseng extract.

[0020] Further preferably, step (1) is specifically as follows: the Panax notoginseng is crushed, mixed with water at a solid-liquid ratio of 1:15 g / mL, the pH is adjusted to 10 using an alkaline substance, the extraction is performed by heating under reflux at 80-90° C. for 4.5 h, filtered, and the filtrate is concentrated and dried to obtain the Panax notoginseng extract.

[0021] Preferably, step (2) is specifically as follows: crushing the astragalus, mixing it with water at a solid-liquid ratio of 15-25 g / mL, and decocting it for 2-4 hours; while still hot, subjecting it to microwave treatment at a power of 30 kW-40 kW for 10-30 minutes, filtering it, and concentrating and drying the filtrate to obtain the astragalus extract.

[0022] Preferably, step (2) comprises the following steps: crushing the astragalus, mixing it with water at a solid-liquid ratio of 1:20 g / mL, and boiling it for 3 h; subjecting it to microwave treatment at a power of 35 kW for 20 min while it is still hot, filtering it, and concentrating and drying the filtrate to obtain the astragalus extract.

[0023] Furthermore, the present invention provides the use of the above-mentioned repair composition in the preparation of pharmaceutical preparations and / or cosmetics with antioxidant, wound healing promoting, anti-inflammatory, skin repairing, and skin acne pigmentation improving effects.

[0024] Then, the present invention provides a cosmetic having the effects of anti-oxidation, promoting wound healing, anti-inflammation, repairing skin and improving skin acne pigmentation, comprising the above-mentioned repairing composition.

[0025] Preferably, the cosmetics are not limited to toner, lotion, essence, cream, mask, and facial cleanser.

[0026] Finally, the present invention provides a pharmaceutical preparation having the effects of anti-oxidation, promoting wound healing, anti-inflammation, repairing skin, and improving skin acne pigmentation, comprising the above-mentioned repair composition.

[0027] Preferably, the pharmaceutical preparation is an external application product acting on the skin, including but not limited to dressings, emulsions, ointments, and sprays.

[0028] Compared with the prior art, the present invention has the following beneficial effects: 1. In the repair composition of the present invention, PN, sodium hyaluronate, palmitic acid tripeptide-5, recombinant human epidermal factor and three extract components interact with each other to bring about the effects of synergistically promoting wound healing, anti-inflammatory, and improving skin acne pigmentation.

[0029] 2. In the repair composition of the present invention, Panax notoginseng extract and Astragalus extract are both prepared by a specific method. The Panax notoginseng extract is heated with an alkaline substance, which is beneficial to the dissolution of the active ingredients. The Astragalus extract is decocted and microwave-assisted to open the fiber channels in the Astragalus, thereby increasing the content of ingredients such as astragalus polysaccharides. The Panax notoginseng extract, Astragalus extract and Calendula officinalis extract complement each other, have no irritation to the skin, and bring significant antioxidant and skin repair effects.

[0030] 3. The present invention uses sodium hyaluronate with a molecular weight of 120kDa-200kDa to prepare the repair composition, so that the wettability and supporting force are improved, which is beneficial to skin repair and wound healing, and can also moisturize the skin and make the skin smooth and delicate. DETAILED DESCRIPTION

[0031] The following non-limiting examples can enable those skilled in the art to more fully understand the present invention, but do not limit the present invention in any way. The following content is merely an exemplary description of the scope of the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, which should also fall within the scope of the present invention.

[0032] When the embodiments give numerical ranges, it should be understood that, unless otherwise specified in the present invention, both endpoints of each numerical range and any numerical value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used in the present invention have the same meanings as those generally understood by those of ordinary skill in the art to which the present invention belongs.

[0033] The present invention is further described below by way of specific examples. Unless otherwise specified, the various chemical reagents used in the examples of the present invention are obtained through conventional commercial channels.

[0034] In the following examples, the palmitoyl tripeptide-5 was purchased from Qingdao Jesmin Trading Co., Ltd. with the item number UQ20352009; the calendula officinalis extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd. with a specification of 20:1; products from different manufacturers had no significant effect on the effect.

[0035] Example 1 A repair composition containing PN and sodium hyaluronate, comprising the following components by weight: 2.5 parts of PN, 45 parts of sodium hyaluronate (molecular weight 160 kDa), 6 parts of palmitoyl tripeptide-5, 3.5 parts of notoginseng extract, 1.2 parts of astragalus extract, 1.2 parts of recombinant human epidermal growth factor and 1.3 parts of calendula extract.

[0036] The preparation method of Panax notoginseng extract is as follows: Panax notoginseng is crushed and passed through an 80-mesh sieve, mixed with water at a solid-liquid ratio of 1:15 g / mL, sodium carbonate is added to adjust the pH of the water to 10, heated and refluxed for extraction at 85±5°C for 4.5 hours, filtered, the filtrate is concentrated, and freeze-dried to obtain the Panax notoginseng extract.

[0037] The preparation method of the astragalus extract is as follows: the astragalus is crushed through an 80-mesh sieve, mixed with water at a solid-liquid ratio of 1:20 g / mL, and decocted for 3 hours; microwave-treated for 20 minutes at a power of 35 kW while hot, filtered, the filtrate is concentrated, and freeze-dried to obtain the astragalus extract.

[0038] The above components are mixed evenly to obtain a repair composition.

[0039] Example 2 A repair composition containing PN and sodium hyaluronate, comprising the following components by weight: 2 parts of PN, 35 parts of sodium hyaluronate (molecular weight 180kDa), 5 parts of palmitoyl tripeptide-5, 3 parts of Panax notoginseng extract, 1 part of Astragalus extract, 1 part of recombinant human epidermal growth factor and 1 part of calendula extract.

[0040] The preparation methods of Panax notoginseng extract and Astragalus extract are the same as those in Example 1.

[0041] The above components are mixed evenly to obtain a repair composition.

[0042] Example 3 A repair composition containing PN and sodium hyaluronate, comprising the following components by weight: 3 parts of PN, 50 parts of sodium hyaluronate (molecular weight 150 kDa), 7 parts of palmitoyl tripeptide-5, 4 parts of notoginseng extract, 1.5 parts of astragalus extract, 1.5 parts of recombinant human epidermal growth factor and 1.5 parts of calendula extract.

[0043] The preparation methods of Panax notoginseng extract and Astragalus extract are the same as those in Example 1.

[0044] The above components are mixed evenly to obtain a repair composition.

[0045] Example 4 A repair composition containing PN and sodium hyaluronate, comprising the following components by weight: 1 part of PN, 30 parts of sodium hyaluronate (molecular weight 200kDa), 3 parts of palmitoyl tripeptide-5, 2.5 parts of notoginseng extract, 0.5 parts of astragalus extract, 0.5 parts of recombinant human epidermal growth factor and 0.5 parts of calendula extract.

[0046] The preparation method of Panax notoginseng extract is as follows: Panax notoginseng is crushed and passed through an 80-mesh sieve, mixed with water at a solid-liquid ratio of 1:10 g / mL, sodium carbonate is added to adjust the pH of the water to 12, heated and refluxed for extraction at 80±5°C for 6 hours, filtered, the filtrate is concentrated, and freeze-dried to obtain the Panax notoginseng extract.

[0047] The preparation method of the astragalus extract is as follows: the astragalus is crushed through an 80-mesh sieve, mixed with water at a solid-liquid ratio of 1:25 g / mL, and decocted for 2 hours; microwave treatment is performed at a power of 30 kW for 30 minutes while hot, filtered, the filtrate is concentrated, and freeze-dried to obtain the astragalus extract.

[0048] The above components are mixed evenly to obtain a repair composition.

[0049] Example 5 A repair composition containing PN and sodium hyaluronate, comprising the following components by weight: 4 parts of PN, 60 parts of sodium hyaluronate (molecular weight 120kDa), 8 parts of palmitoyl tripeptide-5, 5 parts of Panax notoginseng extract, 2 parts of Astragalus extract, 2 parts of recombinant human epidermal growth factor and 2 parts of calendula extract.

[0050] The preparation method of Panax notoginseng extract is as follows: Panax notoginseng is crushed and passed through an 80-mesh sieve, mixed with water at a solid-liquid ratio of 1:20 g / mL, sodium bicarbonate is added to adjust the pH of the water to 9, heated and refluxed for extraction at 90±5°C for 1 hour, filtered, the filtrate is concentrated, and freeze-dried to obtain the Panax notoginseng extract.

[0051] The preparation method of the astragalus extract is as follows: the astragalus is crushed through an 80-mesh sieve, mixed with water at a solid-liquid ratio of 1:15 g / mL, and decocted for 4 hours; microwave treatment is performed at a power of 40 kW for 10 minutes while hot, filtered, the filtrate is concentrated, and freeze-dried to obtain the astragalus extract.

[0052] The above components are mixed evenly to obtain a repair composition.

[0053] Comparative Example 1 Different from Example 1, the repair composition does not contain the components palmitoyl tripeptide-5 and recombinant human epidermal growth factor.

[0054] A repair composition comprises the following components by weight: 2.5 parts of PN, 45 parts of sodium hyaluronate (molecular weight of 160 kDa), 3.5 parts of notoginseng extract, 1.2 parts of astragalus extract and 1.3 parts of calendula extract.

[0055] The rest are the same as in Example 1.

[0056] Comparative Example 2 Different from Example 1, the repair composition does not contain components of Panax notoginseng extract, Astragalus extract and Calendula officinalis extract.

[0057] A repair composition comprises the following components by weight: 2.5 parts of PN, 45 parts of sodium hyaluronate (molecular weight of 160 kDa), 6 parts of palmitoyl tripeptide-5 and 1.2 parts of recombinant human epidermal growth factor.

[0058] The above components are mixed evenly to obtain a repair composition.

[0059] Comparative Example 3 The difference from Example 1 is that palmitoyl tripeptide-5 is replaced by trifluoroacetyl tripeptide-2. The rest is the same as Example 1.

[0060] Comparative Example 4 The difference from Example 1 is that the Panax notoginseng extract is replaced by the evening primrose extract, and the Astragalus extract is replaced by the Centella asiatica extract.

[0061] A repair composition comprises the following components by weight: 2.5 parts of PN, 45 parts of sodium hyaluronate (molecular weight 160kDa), 6 parts of palmitoyl tripeptide-5, 3.5 parts of evening primrose extract, 1.2 parts of Centella asiatica extract, 1.2 parts of recombinant human epidermal growth factor and 1.3 parts of calendula extract.

[0062] The preparation method of evening primrose extract is as follows: evening primrose is crushed through an 80-mesh sieve, mixed with water at a solid-liquid ratio of 1:15 g / mL, sodium carbonate is added to adjust the pH of the water to 10, heated and refluxed for extraction at 85±5°C for 4.5 hours, filtered, the filtrate is concentrated, and freeze-dried to obtain the evening primrose extract.

[0063] The preparation method of Centella asiatica extract is as follows: the Centella asiatica is crushed through an 80-mesh sieve, mixed with water at a solid-liquid ratio of 1:20 g / mL, and decocted for 3 hours; microwave treatment is performed for 20 minutes at a power of 35 kW while hot, filtered, the filtrate is concentrated, and freeze-dried to obtain the Centella asiatica extract.

[0064] The above components are mixed evenly to obtain a repair composition.

[0065] Comparative Example 5 The difference from Example 1 is that the weight proportions of the components in the repair composition are different.

[0066] A repair composition comprises the following components by weight: 1.5 parts of PN, 56.2 parts of sodium hyaluronate (molecular weight 160 kDa), 1 part of palmitoyl tripeptide-5, 0.5 parts of notoginseng extract, 0.5 parts of astragalus extract, 0.5 parts of recombinant human epidermal growth factor and 0.5 parts of calendula extract.

[0067] The rest are the same as in Example 1.

[0068] Comparative Example 6 Different from Example 1, the preparation methods of Panax notoginseng extract and Astragalus membranaceus extract are different.

[0069] Preparation method of Panax notoginseng extract: Panax notoginseng is crushed and passed through an 80-mesh sieve, mixed with a solid-liquid ratio of 1:15 g / mL, heated under reflux extraction at 85±5°C for 4.5 hours, filtered, the filtrate is concentrated, and freeze-dried to obtain Panax notoginseng extract.

[0070] Preparation method of astragalus extract: Astragalus is crushed and passed through an 80-mesh sieve, mixed with water at a solid-liquid ratio of 1:20 g / mL, boiled for 3 hours, filtered, the filtrate is concentrated, and freeze-dried to obtain the astragalus extract.

[0071] The rest is the same as in Example 1.

[0072] Experiment 1 DPPH free radical scavenging rate experiment (1) Reagent preparation Preparation of the test solution: dilute the composition of each example and comparative example with deionized water to a composition mass concentration of 5.0%.

[0073] DPPH ethanol solution: Weigh 20 mg DPPH, dissolve in anhydrous ethanol and dilute to 1000 mL in a volumetric flask. The DPPH concentration is 20 mg / L. Store at 0-5°C away from light and use immediately after preparation.

[0074] (2) Experimental methods Take 1.0 mL of the test solution and mix it with 2.0 mL of 20 mg / L DPPH solution to a total volume of 3 mL. The resulting solution is the sample group, and the measured absorbance is recorded as A1; Take 2.0mL of anhydrous ethanol and 1.0mL of the test solution and mix them evenly to a total volume of 3mL. The resulting solution is the negative control group, and the measured absorbance is recorded as A2; Take 1.0 mL of anhydrous ethanol and 2.0 mL of 20 mg / L DPPH solution and mix them to a total volume of 3 mL. The resulting solution is the blank control group, and the measured absorbance is recorded as A3; After reacting for 30 min, take 1 mL and measure the absorbance of A1, A2 and A3 at 517 nm.

[0075] The calculation formula of DPPH free radical scavenging rate (Clearance Rate) is: DPPH free radical scavenging rate (%) = [1-(A1-A2) / A3] × 100%; where A1 is the sample absorbance, i.e., the absorbance of the solution after the test solution reacts with DPPH; A2 is the absorbance of the blank control group, i.e., the absorbance of the solution after the test solution reacts with anhydrous ethanol; A3 is the absorbance of the negative control group, i.e., the absorbance of the solution after the reaction of anhydrous ethanol and DPPH.

[0076] The DPPH free radical scavenging results of each composition are shown in Table 1.

[0077] Table 1

[0078] In Table 1, each comparative example group is compared with the example 1 group. # P≤0.05, ## P≤0.01, ### P≤0.001, with significant difference.

[0079] As can be seen from Table 1, the repair composition of the embodiment of the present invention has a significantly improved free radical scavenging ability. Comparative Example 1 does not contain the components palmitoyl tripeptide-5 and recombinant human epidermal growth factor, and Comparative Example 2 does not contain the components Panax notoginseng extract, Astragalus extract and Calendula officinalis extract. The lack of components in the composition has a very significant decrease in the scavenging rate of free radicals, and the sum of the free radical scavenging effects of Comparative Example 1 and Comparative Example 2 is still lower than that of the embodiment of the present invention. Therefore, the components in the repair composition of the present invention are mutually synergistic, and each component is indispensable. Comparative Example 3 replaces palmitoyl tripeptide-5 with trifluoroacetyl tripeptide-2, and Comparative Example 4 replaces Panax notoginseng extract with evening primrose extract and Astragalus extract with Centella asiatica extract. The free radical scavenging rate decreases significantly, indicating that specific ingredients can achieve significantly improved antioxidant effects. After the weight proportions of the components in the composition were changed in Comparative Example 5, the free radical scavenging rate decreased significantly; after the preparation methods of the Panax notoginseng extract and the Astragalus extract were changed in Comparative Example 6, the free radical scavenging rate decreased significantly; this indicates that the ratio of the components in the composition and the specific preparation method of the extract have a significant effect on the free radical scavenging rate.

[0080] The repair composition obtained by the extract components obtained by specific components, ratios, and specific preparation methods of the present invention can effectively improve skin oxidation problems caused by oxidative damage and has a significant effect on repairing the skin.

[0081] Experiment 2: Wound healing and anti-inflammatory effects The repair compositions of the examples and comparative examples were respectively taken and tested in relevant animal tests according to the following skin wound repair test protocol.

[0082] Establishment of mouse skin wound model: The mice were anesthetized with ether, and the hair on the back was clipped. The hair on the back was shaved with a razor, and the skin was cleaned and disinfected with 70% ethanol. A circular mark slightly larger than 1 cm in diameter was made at the same position on both sides of the spine. A full-thickness skin wound was made in the circular mark with a 1 cm diameter skin biopsy punch under sterile conditions. After modeling, the wound was exposed and the mice were kept in a single cage. The day of injury was recorded as day 0.

[0083] Experimental animal grouping: SPF-grade 18-22g male Kunming mice were randomly divided into 12 groups after adaptive feeding for 1 week, with 8 mice in each group: Example 1-Example 5 groups, Comparative Example 1-Comparative Example 6 groups, and model control group. Example 1-Example 5 groups and Comparative Example 1-Comparative Example 6 groups respectively used the corresponding repair composition (sterilized by ultraviolet irradiation before use) to treat mouse skin wounds, and the daily dosage of the repair composition was 0.2g. The model control group was smeared with distilled water. The wound healing was observed within 7 days. On the last day, blood was collected from the orbital venous plexus of mice in each group, and the EILSA kit was used to detect the content of mouse serum inflammatory factor IL-1β.

[0084] Mouse skin wound healing rate (%) = (original wound area - unhealed wound area) / original wound area × 100%.

[0085] Wound healing (complete epithelialization of wound surface) standard: Complete healing occurs when the healed area is greater than 95% of the original wound area, or the wound area is less than 5% of the original wound area.

[0086] The results of wound healing rate and inflammatory factor IL-1β content are shown in Table 2.

[0087] Table 2

[0088] In Table 2, each experimental group was compared with the model control group. # P≤0.05, ## P≤0.01, ### P≤0.001, there is a significant difference; each comparative example group is compared with the example 1 group, △ P≤0.05, △△ P≤0.01, there is a significant difference.

[0089] As can be seen from Table 2, the repair composition of the present invention can achieve the effect of complete wound healing within one week, and the repair composition of the comparative group has a slower wound healing speed. It can be seen from the technical effects of Comparative Examples 1-2 that when the repair composition lacks specific components, the wound healing rate and anti-inflammatory effect decrease significantly, and the specific 7 ingredients of the embodiment bring about a synergistic effect; Comparative Example 3 replaces palmitoyl tripeptide-5 with trifluoroacetyl tripeptide-2, and Comparative Example 4 replaces Panax notoginseng extract with evening primrose extract and Astragalus extract with Centella asiatica extract, and the wound healing rate and anti-inflammatory effect decrease significantly, indicating that the components of the repair composition of the present invention as a whole cannot be replaced at will. After the weight of each component in the composition is changed in Comparative Example 5, the wound healing rate and anti-inflammatory effect decrease significantly; after the preparation method of Panax notoginseng extract and Astragalus extract is changed in Comparative Example 6, the wound healing rate decreases and the content of inflammatory factor IL-1β increases; it shows that the ratio of each component in the composition and the specific preparation method of the extract have a significant effect on wound repair.

[0090] In summary, the repair composition of the present invention has significant anti-inflammatory and wound healing effects.

[0091] Test 3 Human Use Evaluation In accordance with the human skin patch test method specified in Chapter 7, Section 2 of the "Technical Specification for Safety of Cosmetics" (2015 Edition), 10% aqueous solutions of the repair compositions of Examples 1 to 3, Comparative Example 1, Comparative Example 2 and Comparative Example 4 were subjected to human patch tests.

[0092] The number of test volunteers was 36 (half male and half female, aged 18-30 years old), all of whom showed moderate to severe acne pigmentation on their faces. The volunteers were randomly divided into 6 groups, each with 6 people, divided into: Example 1 Group, Example 2 Group, Example 3 Group, Comparative Example 1 Group, Comparative Example 2 Group, Comparative Example 4 Group. A repair composition was used respectively, and the skin condition was checked within 72 hours after using each repair composition. The results showed that no adverse skin reactions were observed, which shows that the repair composition of the present invention is mild and non-irritating to the skin.

[0093] To verify the repair effect of the repair composition of the present invention on the facial acne skin of volunteers, each group of volunteers applied 1g of each repair composition every day for 4 weeks. The acne repair was observed and the improvement effect of acne pigmentation was evaluated. Each volunteer filled in a satisfaction rating questionnaire.

[0094] All volunteers received uniform scoring and evaluation training.

[0095] The scoring criteria are shown in Table 3. The skin condition gradually improves from "very severe" to "mild". The lower the score, the higher the satisfaction with repairing acne pigmentation.

[0096] Table 3

[0097] The scoring results of each volunteer are shown in Table 4.

[0098] Table 4

[0099] In Table 4, the comparative example after 4 weeks of application was compared with the Example 1 group. # P≤0.05, there is a significant difference.

[0100] As can be seen from Table 4, after the volunteers used the repair composition of the present invention for 4 weeks, the acne pigmentation improved from "moderate" and "severe" to "mild"; after the repair compositions of Comparative Examples 1 and 2 with changed components, although the acne pigmentation was slightly improved, the level was still "moderate"; after using the repair composition of Comparative Example 4, the satisfaction with acne pigmentation improved, but 33% of the volunteers still rated it as "moderate".

[0101] The repair composition of the present invention has the technical effect of significantly reducing acne pigmentation and improving user satisfaction.

[0102] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A repair composition containing PN and sodium hyaluronate, characterized in that: The components in parts by weight include: 1-4 parts of PN, 30-60 parts of sodium hyaluronate, 3-8 parts of palmitoyl tripeptide-5, 2.5-5 parts of Panax notoginseng extract, 0.5-2 parts of Astragalus extract, 0.5-2 parts of recombinant human epidermal growth factor and 0.5-2 parts of calendula extract.

2. The repair composition according to claim 1, characterized in that The components in parts by weight include: 2-3 parts of PN, 35-50 parts of sodium hyaluronate, 5-7 parts of palmitoyl tripeptide-5, 3-4 parts of Panax notoginseng extract, 1-1.5 parts of Astragalus extract, 1-1.5 parts of recombinant human epidermal growth factor and 1-1.5 parts of calendula extract.

3. The repair composition according to claim 2, characterized in that The components in parts by weight include: 2.5 parts of PN, 45 parts of sodium hyaluronate, 6 parts of palmitoyl tripeptide-5, 3.5 parts of Panax notoginseng extract, 1.2 parts of Astragalus extract, 1.2 parts of recombinant human epidermal growth factor and 1.3 parts of calendula extract.

4. The repair composition according to claim 1, characterized in that The weight ratio of PN, sodium hyaluronate, palmitic acid tripeptide-5 and recombinant human epidermal factor is 2-3:35-50:5-7:1-1.

5.

5. The repair composition according to any one of claims 1 to 3, characterized in that The molecular weight of the sodium hyaluronate is 120kDa-200kDa.

6. The method for preparing the repair composition according to any one of claims 1 to 5, characterized in that: Includes steps: (1) Preparation method of Panax notoginseng extract: Panax notoginseng and alkaline substances are subjected to heating treatment to obtain Panax notoginseng extract; (2) Preparation method of astragalus extract: Astragalus is decocted in water and then subjected to microwave treatment to obtain the astragalus extract; (3) Mixing the Panax notoginseng extract, the Astragalus extract and other components in the composition to obtain a repair composition.

7. The preparation method according to claim 6, characterized in that: Step (1) specifically comprises: crushing Panax notoginseng, mixing it with water at a solid-liquid ratio of 1:10-20 g / mL, adjusting the pH to 9-12 with an alkaline substance, heating and refluxing extraction at 75-95° C. for 4-6 hours, filtering, concentrating and drying the filtrate to obtain a Panax notoginseng extract; Step (2) is specifically as follows: crushing the astragalus, mixing it with water at a solid-liquid ratio of 15-25 g / mL, and boiling it for 2-4 hours; while still hot, subjecting it to microwave treatment at a power of 30 kW to 40 kW for 10-30 minutes, filtering it, and concentrating and drying the filtrate to obtain the astragalus extract.

8. Use of the repair composition according to any one of claims 1 to 5 in the preparation of pharmaceutical preparations and / or cosmetics having antioxidant, wound healing, anti-inflammatory, skin repair, and skin acne pigmentation improvement effects.

9. A cosmetic having the effects of anti-oxidation, promoting wound healing, anti-inflammatory, repairing skin, and improving skin acne pigmentation, characterized in that: Comprising components: the repair composition according to any one of claims 1 to 5.

10. A pharmaceutical preparation having the effects of anti-oxidation, promoting wound healing, anti-inflammatory, repairing skin, and improving skin acne pigmentation, characterized in that: Comprising components: the repair composition according to any one of claims 1 to 5.

Images