Application of acetyl tetrapeptide-5 in preparation of oil control product

Abstract

The invention provides an application of acetyl tetrapeptide-5 in preparation of an oil control product. According to the invention, the effect of acetyl tetrapeptide-5 on human sebaceous gland cells (SZ95) is researched through cell experiments. The compound reduces the lipid content.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to application of acetyl tetrapeptide-5 in the preparation of oil-control products. Background Art

[0002] Sebaceous glands are multi-lobed glandular structures. Their main function is to produce sebum. Under normal physiological conditions, sebum contributes to the function of the skin because they reduce transepidermal water loss, bring antioxidants to its surface, and have antimicrobial activity. However, overactivity of the sebaceous glands leads to excessive sebum secretion, which can affect the inflammatory process. Alterations in normal physiological processes can lead to pathological conditions such as seborrhea. The secretion of sebum can produce the development of conditions such as acne and seborrheic dermatitis. Currently, isotretinoin can effectively reduce lipid production.

[0003] Isotretinoin can be used to treat acne and seborrheic dermatitis. However, it can cause dry skin, pancreatitis, hepatitis, cataracts, etc. Therefore, it is of great significance to find a new compound that inhibits lipid production. Summary of the invention

[0004] In view of this, the technical problem to be solved by the present invention is to provide an application of acetyl tetrapeptide-5 in the preparation of oil control products.

[0005] Acetyl tetrapeptide-5, also known as eye sebaceous peptide, can eliminate edema, improve skin elasticity and smoothness. Inhibit glycation, reduce eye bags and anti-wrinkle. It is used in the field of skin anti-wrinkle and dark circles, but has not yet been used in the field of oil control. The specific information of the polypeptide is shown in Table 1. The present invention studies the effect of acetyl tetrapeptide-5 on human sebaceous gland cells (SZ95) through cell experiments. The compound reduces the lipid content.

[0006] The present invention provides application of acetyl tetrapeptide-5 in preparing oil control products.

[0007] The oil control of the present invention includes reducing the lipid content in human sebaceous gland cells.

[0008] In some embodiments of the present invention, the concentration of acetyl tetrapeptide-5 is 1 to 1000 μg / mL.

[0009] In some embodiments of the present invention, the concentration of acetyl tetrapeptide-5 can be 1 μg / mL, 10 μg / mL, 100 μg / mL, 1000 μg / mL; or a range between any two of the above.

[0010] The present invention also provides an oil control product, comprising acetyl tetrapeptide-5, a soothing component and auxiliary materials.

[0011] According to the present invention, the auxiliary materials include moisturizers and emulsifiers.

[0012] In the present invention, the moisturizer is glycerin, propylene glycol and squalane; the emulsifier includes palmitoyl isopropyl ester or sorbitan ester; and the soothing ingredient is vitamin B.

[0013] In some embodiments of the present invention, the oil control product comprises the following raw materials in parts by weight:

[0014]

[0015] In some embodiments of the present invention, the oil control product comprises the following raw materials in parts by weight:

[0016]

[0017] According to the present invention, the oil control product is preferably a cosmetic, and the cosmetic is preferably an oil control essence, an oil control essence water or an oil control essence milk.

[0018] The present invention also provides a method for preparing the oil control product according to any one of the above technical solutions, comprising the following steps:

[0019] A) adding acetyl tetrapeptide-5 and vitamin B into distilled water and stirring until dissolved as the aqueous phase component;

[0020] Squalane, isopropyl palmitate and sorbitan ester are mixed evenly to form the oil phase component;

[0021] B) adding the aqueous phase component to the oil phase component to obtain an emulsion;

[0022] C) Add glycerol and propylene glycol to the emulsion to obtain.

[0023] The present invention firstly adds acetyl tetrapeptide-5 and vitamin B into distilled water, and stirs them evenly until they are dissolved, thereby forming a water phase component.

[0024] Squalane, isopropyl palmitate and sorbitan esters were mixed evenly to form the oil phase component.

[0025] The present invention does not limit the sources of the above raw materials, and they can be commercially available. The present invention does not limit the specific operation of the above stirring, and those skilled in the art can stir evenly.

[0026] The water phase components are added to the oil phase components to obtain an emulsion; in one embodiment of the present invention, the water phase components are slowly added to the oil phase components while continuously stirring until an emulsion is formed.

[0027] Add glycerin and propylene glycol to the emulsion to obtain.

[0028] In one specific embodiment of the present invention, glycerol and propylene glycol are added and stirred until uniform; the mixed emulsion is cooled to room temperature.

[0029] The present invention also provides a method for using the oil control product: applying the product on the skin surface. The application method includes smearing, spraying, and sticking; the application site is the arm, face, neck, back, etc.

[0030] The present invention provides an application of acetyl tetrapeptide-5 in the preparation of an oil control product. The present invention studies the effect of acetyl tetrapeptide-5 on human sebaceous gland cells (SZ95) through cell experiments. The compound reduces the lipid content. The experimental results show that after the oil control product is applied continuously for 14 days, the oil secretion amount is reduced from 4.9g / m 2 Reduced to 2.3g / m 2 . BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 Effects of acetyl tetrapeptide-5 on lipid content. DETAILED DESCRIPTION

[0032] The present invention provides the application of acetyl tetrapeptide-5 in the preparation of oil control products. Those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve it. It should be particularly noted that all similar replacements and modifications are obvious to those skilled in the art, and they all belong to the scope of protection of the present invention. The methods and applications of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications of this article without departing from the content, spirit and scope of the present invention to realize and apply the technology of the present invention.

[0033] It should be understood that the expression "one or more of..." includes each of the items recited after the expression individually and in various different combinations of two or more of the recited items, unless otherwise understood from the context and usage. The expression "and / or" in combination with three or more recited items should be understood to have the same meaning, unless otherwise understood from the context.

[0034] The use of the terms "comprising", "having" or "containing", including their grammatical synonyms, should generally be understood as open and non-restrictive, for example not excluding other unrecited elements or steps, unless otherwise specifically stated or otherwise understood from the context.

[0035] In this application, the term "and / or" describes the association relationship of associated objects, indicating that there may be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. A and B can be singular or plural.

[0036] In this application, "at least one" means one or more, "more than one" means two or more. "At least one of the following" or similar expressions refers to any combination of these items, including any combination of single or plural items.

[0037] It should be understood that the order of steps or the order in which certain actions are performed is not important as long as the present invention remains operable. In addition, two or more steps or actions may be performed simultaneously.

[0038] The use of any and all examples or exemplary language, such as "for example" or "including", herein is intended only to better illustrate the invention and does not limit the scope of the invention unless otherwise claimed. No language in this specification should be construed as indicating that any non-claimed element is essential to the practice of the invention.

[0039] In the present invention, the product is an external preparation, and its dosage forms include solution, lotion, emulsion, powder, ointment, and paste. Among them, the lotion includes water powder or suspension.

[0040] In the present invention, the cosmetics can be divided into: oil control cosmetics, care cosmetics and beauty / modification cosmetics. The cosmetics refer to cosmetics that are applied to the human body surface (such as epidermis, hair, nails, lips, etc.) by smearing, spraying or other similar methods to play a role in cleaning and hygiene or eliminating bad odors.

[0041] The oil-control cosmetics are cosmetics that are applied to the human body surface (such as epidermis, hair, nails, lips, etc.) by smearing, spraying or other similar methods to play a maintenance role.

[0042] Oil-control cosmetics suitable for skin include: facial cleansers, makeup remover water (lotion), cleansing cream (honey), facial masks, floral water, itching powder, talcum powder or bath gel; skin care cosmetics suitable for skin include skin care creams, lotions or toners.

[0043] In one embodiment of the present application, when the formulation of the cosmetic composition is a solution or an emulsion, a solvent, a solubilizer or an emulsifier may be included as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan, but may not be limited thereto.

[0044] In one embodiment of the present application, when the preparation of the cosmetic composition is a suspension, the liquid diluent as a carrier component such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitan esters and polyoxyethylene sorbic acid esters and other suspending agents as bitter esters may include microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth, but may not be limited thereto.

[0045] In one embodiment of the present application, when the formulation of the cosmetic composition is a cleanser containing a surfactant, fatty alcohol sulfates, fatty alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazoline derivatives, methyl taurates, sarcosinates, fatty acid amide ether sulfates, alkylamide betaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glyceryl fatty acid esters, etc. are used as carrier components.

[0046] In addition, the numerical ranges and parameters used to define the present invention are approximate values, and the relevant values ​​in the specific embodiments have been presented as accurately as possible. However, any numerical value inherently inevitably contains standard deviations due to individual test methods. Therefore, unless otherwise expressly stated, it should be understood that all ranges, quantities, values ​​and percentages used in this disclosure are modified by "about". Here, "about" generally means that the actual value is within plus or minus 10%, 5%, 1% or 0.5% of a specific value or range.

[0047] It should be understood that in the various embodiments of the present application, the size of the serial numbers of the above-mentioned processes does not mean the order of execution, some or all of the steps can be executed in parallel or sequentially, and the execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of the present application.

[0048] Some cases are recorded in the embodiments and comparative examples of the present invention, wherein the embodiments show certain implementation modes of the present invention. However, this does not mean that the effects of the present invention can only be achieved in these cases.

[0049] In order to further illustrate the present invention, the application of acetyl tetrapeptide-5 provided by the present invention in the preparation of oil control products is described in detail below in conjunction with examples.

[0050] Example 1

[0051] Acetyl tetrapeptide-5, also known as eye serine peptide, can eliminate edema and improve skin elasticity and smoothness. It inhibits glycation, reduces eye bags and anti-wrinkle. It is used in the field of skin anti-wrinkle and dark circles, but has not yet been used in the field of oil control. The specific information of the polypeptide is shown in Table 1. The present invention studies the effect of acetyl tetrapeptide-5 on human sebaceous gland cells (SZ95) through cell experiments. The compound reduces the lipid content. Therefore, the present invention applies for the application of acetyl tetrapeptide-5 in the field of oil control.

[0052] Table 1: Basic information of acetyl tetrapeptide-5

[0053]

[0054]

[0055] The raw materials and reagents used in the examples are generally commercially available.

[0056] Among them, human sebaceous gland cells (SZ95) came from Qingqi (Shanghai) Biotechnology Development Co., Ltd.; oleic acid came from Shanghai Aladdin Biochemical Technology Co., Ltd.; DMEM culture medium came from HyClone; serum came from GibcoLife Technologies; penicillin-streptomycin dual antibody came from Gibco Life Technologies; lipid droplet red fluorescence detection kit came from Shanghai Beyotime Biotechnology Co., Ltd.

[0057] Acetyl Tetrapeptide-5 has an oil-control effect

[0058] Experimental procedure: 96 black-well plates (SZ95, P8) were plated with a density of 10,000 sebaceous gland cells per well. After 24 hours, 100 μM oleic acid was added to all groups except the Control group, and the culture was continued for 24 hours. Then, the positive control (isoretinoic acid, concentration of 100 μg / mL) and acetyl tetrapeptide-5 (concentrations of 1, 10, 100, and 1000 μg / mL) were added, and the culture was continued for 24 hours. The cells were washed once with PBS, and then stained with a lipid droplet red fluorescence detection kit for 20 minutes. The cells were washed twice with PBS, and the fluorescence intensity was detected (Ex: 552 nm, 636 nm).

[0059] Experimental results: Figure 1 Effects of acetyl tetrapeptide-5 on lipid content. Figure 1 It can be seen that compared with the control group, the relative oil content increased by 52% after the addition of oleic acid. Compared with the negative control group, after the addition of 1, 10, 100, and 1000 μg / mL of acetyl tetrapeptide-5, the oil content decreased by 67%, 70%, 76%, and 87%, respectively, and it was statistically significant (P<0.05). This shows that acetyl tetrapeptide-5 has the effect of reducing oil and has an oil control effect.

[0060] Embodiment 2:

[0061] Formula preparation: First, prepare the raw materials and formulation ratio according to Table 2. Add acetyl tetrapeptide-5 and vitamin B into distilled water and stir until dissolved as the water phase component; in another container, mix squalane, isopropyl palmitate and sorbitol esters evenly as the oil phase component. Slowly add the water phase component to the oil phase component while stirring continuously until an emulsion is formed; add glycerin and propylene glycol and continue stirring until uniform; after the mixed emulsion cools to room temperature, conduct a final inspection to ensure the stability of the formula.

[0062] Table 2 Oil control essence formula

[0063]

[0064]

[0065] Test results: Volunteers with oily skin were selected as experimental subjects. The volunteers' facial skin was cleaned before the experiment, and the basic skin oil level was recorded. The oil control essence was applied to different areas of the volunteers for four hours and used continuously for 14 days. After each application for 4 hours, a quantitative test was performed using a skin oil test paper. As shown in Table 3, after continuous application of the oil control essence for 14 days, the oil secretion amount increased from 4.9g / m 2 Reduced to 2.3g / m 2 .

[0066] Table 3: Oil secretion

[0067]

[0068] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. Application of acetyl tetrapeptide-5 in the preparation of oil control products.

2. The use according to claim 1, characterized in that: The oil control includes reducing the lipid content in human sebaceous gland cells.

3. The use according to claim 1, characterized in that: The concentration of the acetyl tetrapeptide-5 is 1 to 1000 μg / mL.

4. An oil control product, characterized in that: Comprising acetyl tetrapeptide-5 and excipients.

5. The oil control product according to claim 4, characterized in that: The auxiliary materials include: moisturizer, emulsifier and soothing ingredients.

6. The oil control product according to claim 4, characterized in that: The moisturizers are glycerin, propylene glycol and squalane; the emulsifiers include palmitoyl isopropyl ester or sorbitol ester; and the soothing ingredient is vitamin B.

7. The oil control product according to claim 4, characterized in that: The oil control product comprises the following raw materials in parts by weight:

8. The oil control product according to claim 7, characterized in that: The oil control product comprises the following raw materials in parts by weight:

9. The oil control product according to claim 4, characterized in that: The oil control product is an oil control essence, an oil control essence water or an oil control essence milk.

10. A method for preparing the oil control product according to any one of claims 4 to 9, characterized in that: The steps include: A) adding acetyl tetrapeptide-5 and vitamin B into distilled water and stirring until dissolved as the aqueous phase component; Squalane, isopropyl palmitate and sorbitan ester are mixed evenly to form the oil phase component; B) adding the aqueous phase component to the oil phase component to obtain an emulsion; C) Add glycerol and propylene glycol to the emulsion to obtain.

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