Traditional Chinese medicine composition for consolidating body resistance and resisting aging and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a foundation-consolidating and anti-aging traditional Chinese medicine composition and a preparation method thereof. The traditional Chinese medicine composition is prepared from corn stigma, semen cuscutae, oysters, dogwood, gordon euryale seeds, white poria cocos, radix asparagi, lotus seeds, polygala tenuifolia pulp, ophiopogon roots, prepared rehmannia roots, schisandra chinensis, radix morindae officinalis, semen plantaginis, common yam rhizomes, fructus lycii, radix codonopsis, costus roots, broussonetia papyrifera leaves, platycladi seeds, raspberries, Chinese dates, Chinese yams, ginseng, codonopsis pilosula and salvia miltiorrhiza. The aging process can be slowed down, and the service life can be prolonged.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a strengthening and anti-aging traditional Chinese medicine composition and a preparation method thereof. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention, and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.

[0003] Aging is a natural physiological process, which is the result of the gradual loss of function of cells and tissues in the long-term life of organisms. With the increase of age, the metabolic function, immune system and endocrine system of the human body gradually decline, resulting in the gradual decline of the function of tissues and organs, thus causing various geriatric diseases, such as cardiovascular disease, diabetes, osteoporosis, etc. The mechanism of aging involves many factors, including oxidative stress, free radical damage, gene mutation, endocrine disorders and cell apoptosis.

[0004] According to TCM theory, aging is caused by imbalance of yin and yang, insufficient qi and blood, and decreased function of internal organs. Chinese medicine can effectively delay the aging process by regulating qi and blood, nourishing yin and tonifying the kidney, and promoting blood circulation and removing blood stasis. The advantage of Chinese medicine lies in its overall conditioning characteristics, which can improve the body's functions through multiple effects, thereby achieving anti-aging effects. In recent years, with the development of modern science and technology, more and more Chinese medicine ingredients have been found to have significant anti-aging effects. For example, ginseng, wolfberry, astragalus, ganoderma lucidum and other medicinal materials have been proven to have the effects of enhancing immunity, anti-oxidation, and delaying cell aging. In addition, the research on Chinese medicine prescriptions is also deepening, and many traditional Chinese medicine prescriptions have been found to have good anti-aging effects.

[0005] A Chinese patent with publication number CN1491714A discloses a Chinese medicine composition, which is made of Chinese medicines including lotus seeds, Euryale ferox, yam, Poria cocos, wolfberry, white hyacinth bean, Morus alba, Rehmannia glutinosa, ginseng, Cordyceps sinensis, and Rhodiola rosea. It has anti-aging and strengthening effects, is suitable for sub-healthy people and people with sexual disorders, and can significantly enhance human immunity.

[0006] The Chinese patent with publication number CN106362005A discloses a sugar-free anti-aging oral liquid, including 12-18 parts of ginseng, 12-18 parts of wolfberry, 8-13 parts of yam, 6-10 parts of schisandra, 12-18 parts of asparagus, 12-18 parts of ophiopogon, 12-18 parts of raw rehmannia, 12-18 parts of cooked rehmannia. 1-28 parts, 18-28 parts of Polygonum multiflorum, 12-18 parts of Poria, 12-18 parts of Achyranthes bidentata, 12-18 parts of Angelica sinensis, 12-18 parts of Psoralea corylifolia, 12-18 parts of Eucommia ulmoides, 12-18 parts of Morinda officinalis, 10-15 parts of Anemarrhena asphodeloides, 10-15 parts of Phellodendron chinense, 5-10 parts of Curculigo orchioides, 5-10 parts of Epimedium, 18-28 parts of Alismatis, 12-18 parts of Salvia miltiorrhiza, 12-18 parts of Astragalus, 12-18 parts of Taxillus parasiticus, 15-20 parts of Ligustrum lucidum, 16-24 parts of Ecliptae, 5-8 parts of Cinnamon bark, 8-12 parts of Dendrobium candidum, 0.2-3 parts of sweetener, 0.5-2 parts of ethylparaben and 1-3 parts of sodium benzoate.

[0007] Although Chinese medicine has made some progress in the field of anti-aging, the existing anti-aging Chinese medicine preparations still have the following problems: (1) Insufficient validation of efficacy: The efficacy of many traditional Chinese medicine anti-aging prescriptions lacks systematic validation and evaluation, which affects the credibility of their widespread application. (2) Poor efficacy: Traditional Chinese medicine preparations do not fully extract their active ingredients, resulting in poor effects. The extraction method needs to be further improved. Summary of the invention

[0008] In order to overcome the shortcomings of the prior art, the present invention provides a Chinese medicine composition. The Chinese medicine composition of the present invention is designed mainly for anti-aging, prolonging life, and enhancing immunity. Through the comprehensive effects of multiple medicinal materials, the effects of strengthening physical fitness, improving immunity, nourishing yin and kidney, calming the nerves and stabilizing the mind are achieved.

[0009] The formula of the Chinese medicine composition of the present invention is: 150-250 weight parts of corn silk, 350-450 weight parts of dodder seeds, 180-220 weight parts of oysters, 170-230 weight parts of cornus officinalis, 250-350 weight parts of euryale seeds, 150-250 weight parts of white tuckahoe, 75-120 weight parts of asparagus, 180-240 weight parts of lotus seeds, 90-130 weight parts of polygala root, 80-130 weight parts of ophiopogon, 90-140 weight parts of rehmannia root, 50-150 weight parts of schisandra chinensis, 35-120 weight parts of euryale ferox, 100-150 weight parts of schisandra chinensis, 150-250 weight parts of schisandra chinensis, 75-120 weight parts of asparagus, 180-240 weight parts of lotus seeds, 90-130 weight parts of polygala root, 80-130 weight parts of ophiopogon, 90-140 weight parts of rehmannia root, 50-150 weight parts of schisandra chinensis, 35-120 weight parts of euryale ferox, 100-150 weight parts of schisandra chinensis ... 85 weight parts, Plantago seed 110 ~ 180 weight parts, Chinese yam 75 ~ 125 weight parts, wolfberry fruit 70 ~ 150 weight parts, Zhengfangdang 80 ~ 125 weight parts, Guangmuxiang 50 ~ 110 weight parts, Broussonetia papyrifera leaves 125 ~ 170 weight parts, Platycladus orientalis seeds 80 ~ 130 weight parts, Rubus idaeus 120 ~ 180 weight parts, jujube (without kernel), yam 380 ~ 450 weight parts, ginseng 150 ~ 240 weight parts, Codonopsis pilosula 70 ~ 120 weight parts, Salvia miltiorrhiza 80 ~ 125 weight parts.

[0010] King medicine: Ginseng: Greatly replenishes vitality, strengthens the body and resists fatigue.

[0011] Codonopsis: tonifies the middle and replenishes Qi, strengthens the spleen and stomach, and enhances physical strength.

[0012] Plantago seed: diuretic, heat-clearing and detoxifying, removes toxins from the body and fights aging.

[0013] Paper mulberry leaves: clear away heat and detoxify, improve blood circulation, help the body eliminate toxins, and help anti-aging.

[0014] Minister medicine: Chinese Yam: Strengthens the spleen and stomach, nourishes the lungs and kidneys, strengthens the internal organs, and helps ginseng and codonopsis to improve qi and blood.

[0015] White Poria: promotes diuresis and eliminates dampness, strengthens the spleen and calms the mind, and helps improve the function of the digestive system.

[0016] Gorgon fruit: strengthens the spleen and stops diarrhea, tonifies the kidneys and consolidates essence, promotes diuresis and reduces swelling, and helps regulate the spleen and stomach.

[0017] Rehmannia root: nourishes yin and kidney, replenishes blood and essence, and is combined with ginseng and codonopsis to enhance the tonic effect.

[0018] Wolfberry: nourishes the liver and kidneys, improves sperm quality and improves eyesight, and assists in nourishing the kidneys and blood.

[0019] Salvia miltiorrhiza: It can invigorate blood circulation, remove blood stasis, and improve blood circulation, which helps to improve overall health.

[0020] Jujube: replenishes qi, nourishes blood and soothes the mind, harmonizes other medicines, and strengthens the body.

[0021] Adjuvant: Lotus seeds: tonify the spleen and stop diarrhea, nourish the heart and calm the mind, and coordinate the functions of the spleen and stomach.

[0022] Polygala root: calms the mind, enhances memory, and helps overall health.

[0023] Ophiopogon japonicus: nourishes yin and moistens the lungs, calms the mind, and alleviates the irritation of qi-tonifying drugs.

[0024] Cornus officinalis: nourishes the liver and kidneys, consolidates essence and reduces urination, and enhances the tonic effect.

[0025] Cuscuta: Nourishes the liver and kidneys, improves essence and blood, and improves overall health.

[0026] Raspberry: Nourishes the liver and kidneys, consolidates semen and reduces urination, supports kidney function and the immune system.

[0027] Drug: Guangmuxiang: Promotes qi and relieves pain, aids digestion, and helps the overall coordination of drugs.

[0028] Platycladus orientalis: calms the mind and nourishes the heart, moistens the intestines and promotes bowel movements, and helps regulate the effects of traditional Chinese medicine.

[0029] Zhengfang Party: Strengthen the tonic effect and improve overall health status.

[0030] Morinda officinalis: nourishes the kidney and strengthens yang, strengthens tendons and bones, and helps improve the overall effect.

[0031] Solution: This prescription achieves the effects of anti-aging, prolonging life, and improving immunity by replenishing qi and blood, nourishing the liver and kidneys, calming the mind, and removing toxins and silting. Ginseng and Codonopsis are used as the main medicines to replenish qi and blood and strengthen the body. Plantago and Broussonetia scoparia are used as the main medicines to clear away heat and detoxify. Detoxification and waste removal, other herbs such as Rehmannia glutinosa, Chinese yam, white Poria cocos and other auxiliary herbs enhance the nourishment of qi and blood and the recovery of physical strength; Lycium barbarum, lotus seeds, etc. further adjust the functions of the internal organs and improve the overall effect. Enabling herbs such as Guangmuxiang and Morinda officinalis help to reconcile the overall prescription and ensure the smooth performance of the efficacy. The combination of the above-mentioned medicinal flavors plays a role in regulating qi, blood, yin and yang, enhancing immunity, and can delay the aging process.

[0032] The preparation method of the Chinese medicine composition of the present invention is: Step a: Grind ginseng, codonopsis pilosula and paper mulberry leaves into fine powder, pass through a 300-mesh sieve to obtain medicinal powder for later use; Step b: soaking the psyllium seeds in an aqueous solution of amino acids (glycine, tyrosine, threonine, serine, cysteine, L-aspartic acid) with a mass fraction of 5.0% to 10.0% for 0.5 to 1.5 hours, washing, adding 3 to 6 times the volume of 75% to 85% ethanol for 0.5 to 1.5 hours, adding 0.01 to 0.05 times the amount of vitamin E in the psyllium seeds, reflux extraction in a water bath at 55° C. to 65° C. for 1.5 to 2.5 hours, filtering, collecting the filtrate, removing ethanol by vacuum rotary evaporation at 45° C. to 60° C., filtering, and obtaining a psyllium seed extract; Step c: after corn silk, dodder seed, oyster, cornus officinalis, Euryale ferox, white tuckahoe, asparagus, lotus seed, polygala root, ophiopogon, prepared rehmannia root, schisandra chinensis, moringa officinalis, yam, wolfberry, codonopsis pilosula, aucklandia lappa, cypress seed, raspberry, jujube, yam, and salvia miltiorrhiza are processed, decocted for 0.5 to 1.5 hours with 6 to 8 times of water, filtered, and the filtrate is reserved; the filter residue is decocted for 1.5 to 2.5 hours with 4 to 6 times of water, filtered, and the filtrate is collected, the two filtrates are combined, concentrated to a relative density of 1.25 to 1.45, and a water extract is obtained for use; Step d: Evenly mix the water extract, the plantago seed extract and the drug powder, add a proper amount of flavoring agent and / or filler, mix well, and prepare pills.

[0033] The present invention also provides use of the Chinese medicine composition of the present invention in preparing anti-aging drugs.

[0034] The present invention also provides use of the Chinese medicine composition of the present invention in preparing a drug for improving immunity.

[0035] Compared with the prior art, the technical effects of the present invention are: The Chinese medicine composition of the present invention can extend the telomeres of aged mice or delay their shortening, which helps to slow down the aging process and prolong lifespan. In addition, it can also increase the content of immunoglobulins (IgA, IgG, IgM) and cytokines (such as IL-2), which helps to improve the function of the immune system and overall immunity.

[0036] The present invention also optimizes the extraction process, and through further extraction of the plantago seed medicinal material, increases the content of the effective ingredient, which helps to improve the efficacy of the medicine. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 : Extraction effect of glycine aqueous solution with different mass fractions.

[0038] Figure 2 : Extraction effect of threonine aqueous solution with different mass fractions.

[0039] Figure 3 : Relative telomere length of rats in each group.

[0040] Figure 4: Expression of cytokine (IL-2) in serum of rats in each group. DETAILED DESCRIPTION

[0041] In order to make the purpose and technical solution of the present invention clearer, the present invention is further described below in conjunction with embodiments, but the protection scope of the present invention is not limited to these embodiments, and the embodiments are only used to explain the present invention. It should be understood by those skilled in the art that any changes or equivalent substitutions that do not deviate from the concept of the present invention are included in the protection scope of the present invention.

[0042] Optimization of the preparation method of Plantago asiatica extract The quality of the plantago seed extract is calculated according to the content of geniposide and verbascoside. The detection method of geniposide and verbascoside is determined according to the high performance liquid chromatography method (General Rule 0512) of the Chinese Pharmacopoeia: Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel is used as filler; methanol is used as mobile phase A, 0.5% acetic acid solution is used as mobile phase B, and gradient elution is performed according to the provisions in the following table; the detection wavelength is 254nm. The theoretical plate number calculated based on the geniposide peak should be no less than 3000.

[0043] Table 1 Mobile phase elution program Preparation of reference solution: Take appropriate amount of geniposide reference substance and verbascoside reference substance, weigh accurately, place in a brown volumetric flask, add 60% methanol to make a mixed solution containing 0.1 mg of each per 1 ml.

[0044] Preparation of test solution: Take about 1g of the powder of the product (passed through No. 2 sieve), weigh it accurately, put it in a stoppered conical flask, accurately add 50ml of 60% methanol, weigh it, heat and reflux for 2 hours, cool, weigh it again, make up the lost weight with 60% methanol, shake well, filter, and take the filtrate to obtain the solution.

[0045] Determination method: Accurately pipette 10 μl of reference solution and test solution respectively, inject into liquid chromatograph, and determine.

[0046] Optimization screening of amino acid species Scheme A: Soak the plantago seed in a 8.0% glycine aqueous solution for 1 h, wash, add 5 times the volume of 80% ethanol to extract for 1 h, add 0.03 times the amount of vitamin E of the plantago seed, reflux extraction in a water bath at 60°C for 2 h, filter, collect the filtrate, remove ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the plantago seed extract.

[0047] Scheme B: Soak the plantago seeds in an aqueous solution with a mass fraction of 8.0% tyrosine for 1 hour, wash, add 5 times the volume of 80% ethanol to extract for 1 hour, add 0.03 times the amount of vitamin E of the plantago seeds, reflux extraction in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the plantago seed extract.

[0048] Scheme C: Soak the plantago seed in an aqueous solution of threonine with a mass fraction of 8.0% for 1 hour, wash, add 5 times the volume of 80% ethanol to extract for 1 hour, add 0.03 times the amount of vitamin E of the plantago seed, reflux extraction in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the plantago seed extract.

[0049] Scheme D: Soak the plantago seed in an 8.0% serine aqueous solution for 1 h, wash, add 5 times the volume of 80% ethanol to extract for 1 h, add 0.03 times the amount of vitamin E of the plantago seed, reflux in a water bath at 60°C for 2 h, filter, collect the filtrate, remove the ethanol by vacuum rotary evaporation at 45°C-60°C, filter, and obtain the plantago seed extract.

[0050] Scheme E: Soak the plantago seed in an aqueous solution of cysteine ​​with a mass fraction of 8.0% for 1 hour, wash, add 5 times the volume of 80% ethanol to extract for 1 hour, add 0.03 times the amount of vitamin E of the plantago seed, reflux extraction in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove ethanol by reduced pressure rotary evaporation at 45°C-60°C, filter, and obtain the plantago seed extract.

[0051] Scheme F: Soak the plantago seed in an 8.0% by mass L-aspartic acid aqueous solution for 1 h, wash, add 5 times the volume of 80% ethanol to extract for 1 h, add 0.03 times the amount of vitamin E of the plantago seed, reflux extraction in a water bath at 60°C for 2 h, filter, collect the filtrate, remove ethanol by reduced pressure rotary evaporation at 45°C~60°C, filter, and obtain the plantago seed extract.

[0052] Scheme G: Soak the plantago seed in an 8.0% by mass alanine ethanol (95%) solution for 1 h, wash, add 5 times the volume of 80% ethanol to extract for 1 h, add 0.03 times the amount of vitamin E in the plantago seed, reflux in a water bath at 60°C for 2 h, filter, collect the filtrate, remove ethanol by vacuum rotary evaporation at 45°C-60°C, filter, and obtain the plantago seed extract.

[0053] Scheme H: Soak the plantago seed in a leucine ethanol (95%) solution with a mass fraction of 8.0% for 1 hour, wash, add 5 times the volume of 80% ethanol to extract for 1 hour, add 0.03 times the amount of vitamin E of the plantago seed, reflux extraction in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the plantago seed extract.

[0054] Scheme I: Soak the Plantago seed in an ethanol (95%) solution for 1 hour, wash, add 5 times the volume of 80% ethanol to extract for 1 hour, add 0.03 times the amount of vitamin E in the Plantago seed, reflux in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove the ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the Plantago seed extract.

[0055] Scheme J: Soak the Plantago seeds in deionized water for 1 hour, wash, add 5 times the volume of 80% ethanol and extract for 1 hour, add 0.03 times the amount of vitamin E of the Plantago seeds, reflux in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove the ethanol by vacuum rotary evaporation at 45°C-60°C, filter, and obtain the Plantago seeds extract.

[0056] Scheme K: Soak the plantago seed in an 8.0% arginine aqueous solution for 1 h, wash, add 5 times the volume of 80% ethanol to extract for 1 h, add 0.03 times the amount of vitamin E of the plantago seed, reflux extraction in a water bath at 60°C for 2 h, filter, collect the filtrate, remove ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the plantago seed extract.

[0057] Table 2 Contents of geniposide and verbascoside in the extracts of Plantago asiatica in each regimen Geniposidic acid (%) Verbascoside(%) Plan A 0.794 0.595 Plan B 0.717 0.554 Plan C 0.680 0.562 Plan D 0.705 0.549 Plan E 0.772 0.561 Plan F 0.786 0.579 Plan G 0.481 0.284 Scheme H 0.429 0.306 Scheme I 0.402 0.227 Plan J 0.583 0.410 Plan K 0.510 0.364 Table 2 shows the contents of geniposide and verbascoside in the extracts of plan A to plan K. It can be seen that soaking the plantain seeds in glycine, tyrosine, threonine, serine, cysteine, and L-aspartic acid, and then extracting with ethanol, can effectively increase the extraction amount of geniposide and verbascoside and enhance the efficacy of the drug. Studies have found that soaking the plantain seeds with hydrophilic amino acids can play the following roles: (1) Improving solubility: hydrophilic amino acids can form hydrogen bonds or ionic bonds with polar active ingredients in the plantain seeds, increase the solubility of certain ingredients in the solvent, and thus increase the concentration of active ingredients in the extract. (2) Promoting cell wall destruction: amino acids can help destroy cell walls or cell membranes by interacting with polysaccharides and proteins in the medicinal materials, thereby increasing the release rate of active ingredients. (3) Improving extraction efficiency: By increasing the solubility and release rate of active ingredients in the medicinal materials, hydrophilic amino acids can improve the overall extraction efficiency and make the extraction process more efficient. (4) Enhanced stability: The combination of amino acids and active ingredients may help protect these ingredients, reduce degradation or deterioration during the extraction process, and enhance the stability of the extract. In addition, vitamin E has both hydrophilic and hydrophobic characteristics and is an amphiphilic substance that can reduce the surface tension of the active ingredients in the medicinal materials, increase their solubility in the solvent, and make the active ingredients easier to release from the medicinal materials.

[0058] Optimization and screening of mass fraction of amino acid aqueous solution To explore the selection range of the mass fraction of amino acid aqueous solution, we selected glycine aqueous solution with mass fraction of 1.0%, 2.0%, 3.0%, 4.0%, 5.0%, 6.0%, 7.0%, 8.0%, 9.0%, 10.0%, 12.0%, 15.0%, tyrosine aqueous solution with mass fraction of 1.0%, 2.0%, 3.0%, 4.0%, 5.0%, 6.0%, 7.0%, 8.0%, 9.0%, 10.0%, 12.0%, 15.0%, threonine aqueous solution with mass fraction of 1.0%, 2.0%, 3.0%, 4.0%, 5.0%, 6.0%, 7.0%, 8.0%, 9.0%, 10.0%, 12.0%, 15.0%, respectively. .0%, 9.0%, 10.0%, 12.0%, 15.0% serine aqueous solution, 1.0%, 2.0%, 3.0%, 4.0%, 5.0%, 6.0%, 7.0%, 8.0%, 9.0%, 10.0%, 12.0%, 15.0% cysteine ​​aqueous solution, 1.0%, 2.0%, 3.0%, 4.0%, 5.0%, 6.0%, 7.0%, 8.0%, 9.0%, 10.0%, 12.0%, 15.0% L-aspartic acid aqueous solution to soak plantago for 1 hour, wash, add 5 times the volume of 80% ethanol to soak for 1 hour, add 0.03 times the amount of vitamin E of plantago, reflux extraction at 60℃ in a water bath for 2 hours, filter, collect the filtrate, remove ethanol by vacuum rotary evaporation at 45℃~60℃, filter, and obtain plantago extract. The contents of geniposide and verbascoside in the extract of Plantago were determined, and it was found that the content of active ingredients extracted from the Plantago seeds was higher after soaking them in 5.0% to 10.0% aqueous solutions of glycine, tyrosine, threonine, serine, cysteine, and L-aspartic acid. Only the extraction effects of different mass fractions of glycine and threonine aqueous solutions are shown here ( Figure 1 , Figure 2 ).

[0059] Example 1 Chinese medicine composition Formula: 200 grams of corn silk, 400 grams of dodder seed, 200 grams of oyster, 200 grams of cornus officinalis, 300 grams of Euryale ferox, 200 grams of white poria, 100 grams of asparagus, 200 grams of lotus seeds, 100 grams of polygala root, 100 grams of ophiopogon, 100 grams of rehmannia root, 200 grams of schisandra chinensis, 50 grams of Morinda officinalis, 150 grams of plantain seed, 100 grams of yam, 100 grams of wolfberry, 100 grams of codonopsis pilosula, 100 grams of costusroot, 150 grams of mulberry leaves, 100 grams of pine nuts, 150 grams of raspberry, 400 grams of jujube (without kernel), 400 grams of yam, 200 grams of ginseng, 100 grams of codonopsis pilosula, and 100 grams of salvia miltiorrhiza.

[0060] Preparation method: Step a: Grind ginseng, codonopsis pilosula and paper mulberry leaves into fine powder, pass through a 300-mesh sieve to obtain medicinal powder for later use; Step b: soaking the psyllium seeds in a glycine aqueous solution with a mass fraction of 8.0% for 1 hour, washing, adding 5 times the volume of 80% ethanol to extract for 1 hour, adding 0.03 times the amount of vitamin E in the psyllium seeds, reflux extraction in a water bath at 60° C. for 2 hours, filtering, collecting the filtrate, removing ethanol by vacuum rotary evaporation at 45° C. to 60° C., filtering, and obtaining the psyllium seeds extract; Step c: after corn silk, dodder seed, oyster, cornus officinalis, Euryale ferox, white tuckahoe, asparagus, lotus seed, polygala root, ophiopogon, prepared rehmannia root, schisandra chinensis, moringa officinalis, yam, wolfberry, codonopsis pilosula, aucklandia lappa, cypress seed, raspberry, jujube, yam, and salvia miltiorrhiza are processed, decocted for 1 hour with 7 times of water, filtered, and the filtrate is reserved; the filter residue is decocted for 2 hours with 5 times of water, filtered, the filtrate is collected, the two filtrates are combined, concentrated to a relative density of 1.35, and a water extract is obtained for use; Step d: Evenly mix the water extract, the plantago seed extract and the powder, add 85 grams of honey, 50 grams of starch and 15 grams of lactose, mix well and make into pills.

[0061] Example 2 Chinese medicine composition Formula: 150 grams of corn silk, 350 grams of dodder seed, 180 grams of oyster, 170 grams of cornus officinalis, 250 grams of Euryale ferox, 150 grams of white poria, 75 grams of asparagus, 180 grams of lotus seeds, 90 grams of polygala root, 80 grams of ophiopogon, 90 grams of rehmannia root, 50 grams of schisandra, 35 grams of eucommia, 110 grams of plantain seed, 75 grams of yam, 70 grams of wolfberry, 80 grams of codonopsis pilosula, 50 grams of magnolia officinalis, 125 grams of mulberry leaves, 80 grams of cypress seeds, 120 grams of raspberry, 350 grams of jujube (without kernel), 380 grams of yam, 150 grams of ginseng, 70 grams of codonopsis pilosula, and 80 grams of salvia miltiorrhiza.

[0062] Preparation method: Step a: Grind ginseng, codonopsis pilosula and paper mulberry leaves into fine powder, pass through a 300-mesh sieve to obtain medicinal powder for later use; Step b: soaking the psyllium seeds in a 5.0% glycine aqueous solution for 0.5 hours, washing, adding 3 times the volume of 75% ethanol to extract for 0.5 hours, adding 0.01 times the amount of vitamin E in the psyllium seeds, reflux extraction in a water bath at 55° C. for 1.5 hours, filtering, collecting the filtrate, removing ethanol by vacuum rotary evaporation at 45° C. to 60° C., filtering, and obtaining the psyllium seeds extract; Step c: after corn silk, dodder seed, oyster, cornus officinalis, Euryale ferox, white tuckahoe, asparagus, lotus seed, polygala root, ophiopogon, prepared rehmannia root, schisandra chinensis, moringa officinalis, yam, wolfberry, codonopsis pilosula, aucklandia lappa, cypress seed, raspberry, jujube, yam, and salvia miltiorrhiza are processed, decocted for 0.5h with 6 times of water, filtered, and the filtrate is reserved; the filter residue is decocted for 1.5h with 4 times of water, filtered, and the filtrate is collected, the two filtrates are combined, concentrated to a relative density of 1.25, and a water extract is obtained for use; Step d: Evenly mix the water extract, the plantago seed extract and the powder, add 45 grams of sucrose, 10 grams of fructose, 2 grams of sucralose, 20 grams of polyvinyl alcohol and 75 grams of starch, mix well and make into pills.

[0063] Example 3 Chinese medicine composition Formula: 250 grams of corn silk, 450 grams of dodder seed, 220 grams of oyster, 230 grams of cornus officinalis, 350 grams of Euryale ferox, 250 grams of white poria, 120 grams of asparagus, 240 grams of lotus seeds, 130 grams of polygala root, 130 grams of ophiopogon, 140 grams of rehmannia root, 150 grams of schisandra, 85 grams of Morinda officinalis, 180 grams of plantain seed, 125 grams of yam, 150 grams of wolfberry, 125 grams of codonopsis pilosula, 110 grams of magnolia officinalis, 170 grams of mulberry leaves, 130 grams of pine nuts, 180 grams of raspberry, 430 grams of jujube (without kernel), 450 grams of yam, 240 grams of ginseng, 120 grams of codonopsis pilosula, and 125 grams of salvia miltiorrhiza.

[0064] Preparation method: Step a: Grind ginseng, codonopsis pilosula and paper mulberry leaves into fine powder, pass through a 300-mesh sieve to obtain medicinal powder for later use; Step b: soaking the psyllium seeds in a 10.0% glycine aqueous solution for 1.5 hours, washing, adding 6 times the volume of 85% ethanol to extract for 1.5 hours, adding 0.05 times the amount of vitamin E in the psyllium seeds, reflux extraction in a water bath at 65°C for 2.5 hours, filtering, collecting the filtrate, removing ethanol by vacuum rotary evaporation at 45°C-60°C, filtering, and obtaining the psyllium seeds extract; Step c: after corn silk, dodder seed, oyster, cornus officinalis, Euryale ferox, white tuckahoe, asparagus, lotus seed, polygala root, ophiopogon, prepared rehmannia root, schisandra chinensis, moringa officinalis, yam, wolfberry, codonopsis pilosula, aucklandia lappa, cypress seed, raspberry, jujube, yam, and salvia miltiorrhiza are processed, decocted for 1.5 hours with 8 times of water, filtered, and the filtrate is reserved; the filter residue is decocted for 2.5 hours with 6 times of water, filtered, and the filtrate is collected, the two filtrates are combined, concentrated to a relative density of 1.45, and a water extract is obtained for use; Step d: Evenly mix the water extract, the plantago seed extract and the powder, add 115 grams of xylitol, 15 grams of mannitol and 25 grams of starch, mix well and make into pills.

[0065] Anti-aging effect of the Chinese medicine composition of the present invention Telomeres are repetitive DNA sequences located at the ends of chromosomes. They play a role in protecting chromosomes during cell division. Each time a cell divides, the telomeres become shorter, which is considered a sign of cell aging and an evaluation index for measuring life expectancy. Several 18-month-old SD rats were randomly divided into 4 groups, namely, Example 1 Group, Example 2 Group, Example 3 Group, and Blank Group, with 10 rats in each group. After 2 weeks of adaptive feeding, the rats in Example 1 Group, Example 2 Group, and Example 3 Group were gavaged with the corresponding drugs, with a crude drug amount of 8 g / kg, twice a day, and continued to be administered for 2 months. In order to quantify the relative telomere length in the circulating blood of rats, blood genomic DNA was extracted using the VAMNE MagUltra Blood Genomic DNA Extraction Kit (Nanjing Novozan Biotechnology Co., Ltd., DM101-01), and then mixed with SYBR Fast mix (Takara, CN830A) and primers. The telomere length ratio was used to evaluate the telomere length of each group. The telomere length ratio (T / S ratio) is the ratio of the telomeric repeat copy number (T) to the single-copy gene copy number (S) in a sample using quantitative technology. 36B4 was amplified as a single-copy gene. RT-qPCR was performed in an ABI7500 real-time system. Each sample and primer set was run in triplicate, and the relative telomere length was calculated using the 2-∆∆Ct method.

[0066] Figure 3 is the relative telomere length of each group of rats. After administration, the relative telomere length of the rats in Example 1 group, Example 2 group, and Example 3 group was significantly different from that of the rats in the blank group. P <0.01 (calculated using SPSS22.0).

[0067] The Chinese medicine composition of the present invention has the effect of improving immunity Several 18 month old SD rats were randomly divided into 4 groups, namely, Example 1 group, Example 2 group, Example 3 group, and blank group, with 10 rats in each group. After 2 weeks of adaptive feeding, the rats in Example 1 group, Example 2 group, and Example 3 group were gavaged with the corresponding drugs, 8 g / kg in crude drug amount, twice a day, and continued to be administered for 2 months, and the blank group was given normal saline. Rat serum was taken, and the immunoglobulin (IgA, IgG, IgM) and cytokine (IL-2) content of each group of rats were detected according to the operating steps in the ELISA kit manual.

[0068] Table 3 Rat immunoglobulin (IgA, IgG, IgM) content IgA (ng / ml) IgG (ng / ml) IgM (ng / ml) Blank Group 0.32±0.022 0.30±0.033 0.44±0.029 Example 1 Group 0.57±0.031** 0.48±0.027** 0.69±0.025** Example 2 Group 0.53±0.027** 0.44±0.028** 0.61±0.032** Example 3 Group 0.56±0.035** 0.47±0.023** 0.66±0.030** Compared with the blank group, ** P <0.01.

[0069] The normal level and function of immunoglobulins are crucial to immunity. IgA can protect the mucosal surface and prevent pathogen invasion; IgG can fight bacteria and viruses and maintain long-term immune memory; IgM can provide initial defense against new infections. The coordinated action of these immunoglobulins can effectively improve the body's overall immune ability and help defend against various infections and diseases. The Chinese medicine compositions of Examples 1 to 3 of the present invention can significantly increase the content of immunoglobulins (IgA, IgG, IgM) in rats, play a role in enhancing immune function, and have significant differences compared with the blank group ( P <0.01).

[0070] IL-2 is secreted by activated T cells and is an important cytokine that regulates the immune system. It mainly promotes the proliferation and differentiation of T cells, enhances cell-mediated immune responses, and can also stimulate the activity of other immune cells (such as B cells and natural killer cells) to enhance the overall immune response. Figure 4 The expression of cytokines (IL-2) in the serum of rats in each group. The Chinese medicine compositions of Examples 1 to 3 of the present invention can significantly increase the expression of cytokines (IL-2) in the serum of rats, thereby improving the immune function. Compared with the blank group, there is a significant difference ( P <0.01).

[0071] The above data were statistically analyzed using SPSS 22.0 software, and the quantitative data were The comparison among multiple groups was performed by one-way analysis of variance, and the comparison between two groups was performed by independent sample T test. P The difference was statistically significant when the value was <0.05.

Claims

1. A Chinese medicine composition, characterized in that: The traditional Chinese medicine composition consists of corn silk, dodder seed, oyster, cornus officinalis, Euryale ferox, white tuckahoe, asparagus, lotus seed, polygala root, ophiopogon, rehmannia root, schisandra chinensis, moringa officinalis, plantain seed, yam, wolfberry, codonopsis pilosula, costusroot, mulberry leaf, cypress seed, raspberry, jujube, yam, ginseng, codonopsis pilosula and salvia miltiorrhiza.

2. The Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is calculated by weight: 150-250 parts of corn silk, 350-450 parts of dodder seeds, 180-220 parts of oysters, 170-230 parts of cornus officinalis, 250-350 parts of Euryale ferox, 150-250 parts of white tuckahoe, 75-120 parts of asparagus, 180-240 parts of lotus seeds, 90-130 parts of polygala root, 80-130 parts of ophiopogon, 90-140 parts of rehmannia root, 50-150 parts of schisandra chinensis, 35-85 parts of euryale officinalis, 110-180 parts of Chinese yam, 75-125 parts of wolfberry, 70-150 parts of codonopsis pilosula, 50-110 parts of magnolia officinalis, 125-170 parts of paper mulberry leaves, 80-130 parts of cypress seeds, 120-180 parts of raspberries, 350-430 parts of jujube (without kernel), 380-450 parts of yam, 150-240 parts of ginseng, 70-120 parts of codonopsis pilosula, and 80-125 parts of salvia miltiorrhiza.

3. The Chinese medicine composition according to claim 1, characterized in that The Chinese medicine composition is calculated by weight: 200 parts of corn silk, 400 parts of dodder seeds, 200 parts of oysters, 200 parts of cornus officinalis, 300 parts of euryale seeds, 200 parts of white tuckahoe, 100 parts of asparagus, 200 parts of lotus seeds, 100 parts of polygala root, 100 parts of ophiopogon, 100 parts of rehmannia root, 200 parts of schisandra chinensis, 50 parts of moringa officinalis, 150 parts of plantain seeds, 100 parts of yam, 100 parts of wolfberry, 100 parts of codonopsis pilosula, 100 parts of costusroot, 150 parts of paper mulberry leaves, 100 parts of cypress seed, 150 parts of raspberry, 400 parts of jujube (without kernel), 400 parts of yam, 200 parts of ginseng, 100 parts of codonopsis pilosula, and 100 parts of salvia miltiorrhiza.

4. A method for preparing the Chinese medicine composition according to claim 1, characterized in that: The preparation method is: Step a: Grind ginseng, codonopsis pilosula and paper mulberry leaves into fine powder, sieve to obtain medicinal powder, and set aside; Step b: soaking the psyllium seeds in an amino acid aqueous solution with a mass fraction of 5.0% to 10.0% for 0.5 to 1.5 hours, washing, adding 3 to 6 times the volume of 75% to 85% ethanol to extract for 0.5 to 1.5 hours, adding 0.01 to 0.05 times the amount of vitamin E in the psyllium seeds, reflux extraction in a water bath at 55° C. to 65° C. for 1.5 to 2.5 hours, filtering, collecting the filtrate, removing ethanol by vacuum rotary evaporation at 45° C. to 60° C., filtering, and obtaining the psyllium seeds extract; Step c: after corn silk, dodder seed, oyster, cornus officinalis, Euryale ferox, white tuckahoe, asparagus, lotus seed, polygala root, ophiopogon, prepared rehmannia root, schisandra chinensis, moringa officinalis, yam, wolfberry, codonopsis pilosula, aucklandia lappa, cypress seed, raspberry, jujube, yam, and salvia miltiorrhiza are processed, decocted for 0.5 to 1.5 hours with 6 to 8 times of water, filtered, and the filtrate is reserved; the filter residue is decocted for 1.5 to 2.5 hours with 4 to 6 times of water, filtered, and the filtrate is collected, the two filtrates are combined, concentrated to a relative density of 1.25 to 1.45, and a water extract is obtained for use; Step d: Evenly mix the water extract, the plantago seed extract and the drug powder, add a proper amount of flavoring agent and / or filler, mix well, and prepare pills.

5. The method for preparing the Chinese medicine composition according to claim 4, characterized in that: The sieving in step a is a 300 mesh sieve.

6. The method for preparing the Chinese medicine composition according to claim 4, characterized in that: The amino acids are one or more of glycine, tyrosine, threonine, serine, cysteine ​​and L-aspartic acid.

7. The method for preparing the Chinese medicine composition according to claim 4, characterized in that: The flavoring agent is one or more of honey, sucrose, fructose, sucralose, aspartame, and xylitol.

8. The method for preparing the Chinese medicine composition according to claim 4, characterized in that: The filler is one or more of starch, microcrystalline cellulose, mannitol, lactose, and polyvinyl alcohol.

9. Use of the Chinese medicine composition according to claim 1 in the preparation of anti-aging drugs.

10. Use of the Chinese medicine composition according to claim 1 in preparing a drug for improving immunity.

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