Leymus chinensis accelerant and method for repairing grassland by utilizing Leymus chinensis accelerant

Abstract

The invention discloses a leymus chinensis accelerant and a method for repairing grassland by using the leymus chinensis accelerant, and belongs to the technical field of plant accelerant preparation. The method for repairing the grassland through the leymus chinensis accelerant comprises the following steps that P1, the underground part of leymus chinensis is cut up, and a pretreated leymus chinensis bud propagule is obtained; p2, spraying a leymus chinensis accelerant to the pretreated leymus chinensis bud propagules to obtain bud propagule segments; and P3, sowing the propagule section into the natural grassland by using a sowing machine, and repairing the grassland. By spraying the leymus chinensis accelerant, the vigor of leymus chinensis bud propagules can be effectively improved, the growth of leymus chinensis is promoted, and the biomass is increased, so that the restoration of grassland is realized.

Description

Technical Field

[0001] The invention belongs to the technical field of plant promoter preparation, and particularly relates to a leymus chinensis promoter and a method for restoring grassland using the same. Background Art

[0002] As a perennial rhizome grass, Leymus chinensis is known as an important high-quality forage grass in my country due to its wide ecological adaptability, salt-alkali tolerance, high yield, excellent palatability and crude protein content. However, under the influence of long-term natural and human factors, Leymus chinensis grasslands are facing varying degrees of degradation, which directly affects the ecological function of the grassland and the productivity of the forage grass. The coverage of the degraded grassland is reduced, the species diversity is reduced, and the soil structure and fertility are damaged, which in turn affects the stability of the grassland ecosystem and the forage grass yield.

[0003] Patent CN106721146B discloses a sheepgrass promoter and its application, including nitrogen, phosphorus and brassinolide, wherein the mass ratio of nitrogen, phosphorus and brassinolide is 10000: (1000-2500): (0.0001-0.0003). The sheepgrass promoter of the invention is used to restore degraded sheepgrass grassland by promoting the differentiation of sheepgrass tillers, rapidly increasing the proportion of sheepgrass in the community, and restoring the degraded sheepgrass grassland. However, the sheepgrass promoter prepared by this method still has room for improvement in the biomass of sheepgrass. Summary of the invention

[0004] The object of the present invention is to provide a chinensis promoter and a method for repairing grassland using the same, so as to solve the technical problem of poor biomass of chinensis in the prior art.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions: The invention provides a sheep grass promoter and a method for restoring grassland using the same. The promoter consists of the following components in parts by weight: 52-66 parts of sheep manure, 32-46 parts of wheat bran, 2-6 parts of plant extractant, 2-10 parts of composite additives, and 70-90 parts of nitrogen-phosphorus compound fertilizers, wherein the plant extractant is obtained from Sargassum by a method combining extraction and enzymolysis, and the composite additive is prepared from dehydroabietic acid, lithium aluminum hydride, phthalic anhydride, N-bromosuccinimide, sodium azide and triphenylphosphine.

[0006] Preferably, the method for preparing the plant extract comprises the following steps: Q1: Wash the Sargassum with clean seawater, absorb the moisture on its surface with absorbent paper, cut and crush the clean Sargassum, homogenize it with a homogenizer, then add distilled water, heat and extract, centrifuge, collect the supernatant, and obtain the extract; Q2: Mix the extract with the cellulase solution, adjust the pH, heat for enzymolysis, increase the temperature to inactivate the enzyme, centrifuge, collect the supernatant, and obtain the plant extract.

[0007] In the above process, Sargassum is used as raw material, and the plant extract is extracted by combining physical and mechanical extraction and enzymatic hydrolysis.

[0008] Preferably, in Q1, the ratio of clean Sargassum to distilled water is (50-100) g: (150-300) mL. During the homogenization process, the homogenization is performed for 2 min every 5 min, for a total of 3-5 times. The heating and extraction temperature is 35-38° C., the time is 2-4 h, the centrifugal speed is 10000-12000 rpm, and the centrifugal time is 5-8 min.

[0009] Preferably, in Q2, the volume ratio of the extract to the cellulase solution is (1-2): (12-20), the mass fraction of the cellulase solution is 0.6wt%, the pH is adjusted to 4-5, the heating enzymatic hydrolysis temperature is 50-55°C, the enzymatic hydrolysis time is 60-75min, the heating enzyme inactivation temperature is 95-100°C, the time is 3-5min, the centrifugal speed is 8000-12000rpm, and the centrifugal time is 10-15min.

[0010] Preferably, the preparation method of the composite additive comprises the following steps: S1: adding dehydroabietic acid to a container containing tetrahydrofuran, slowly adding lithium aluminum hydride under low temperature stirring conditions, controlling the adding time, reacting after the addition is completed, heating under reflux reaction after the reaction is completed, adding distilled water dropwise under low temperature conditions, then adding sodium hydroxide solution, then adding distilled water dropwise, drying, standing, suction filtering, rotary evaporation, to obtain intermediate 1; adding intermediate 1 to a container, then adding butyric anhydride, heating and stirring to react, washing, separating the liquids, rotary evaporation, to obtain intermediate 2; S2: Add phthalic anhydride to a container filled with N,N-dimethylformamide, stir to dissolve, add chitosan, heat to react under nitrogen protection, cool after the reaction, pour into ice water, centrifuge, wash, filter, and dry to obtain intermediate A; add intermediate A to a container filled with N-methyl-2-pyrrolidone, slowly add N-bromosuccinimide and triphenylphosphine under low temperature, heat to react, cool after the reaction, place in anhydrous ethanol for precipitation, centrifuge, filter, and dry to obtain intermediate B; S3: Add intermediate B, sodium azide and dimethyl sulfoxide to a container, heat the reaction under nitrogen protection, cool down after the reaction, pour into ethanol for precipitation, centrifuge, filter, and dry to obtain intermediate C; add intermediate C, triphenylphosphine and N,N-dimethylformamide to a container, react at room temperature under nitrogen protection, then add hydrazine hydrate, heat the reaction, cool down, pour into ethanol for precipitation, centrifuge, filter, and dry to obtain intermediate D; S4: adding the intermediate 2 and intermediate D obtained into a container filled with distilled water in sequence, stirring and reacting, and obtaining a composite additive.

[0011] In the above process, firstly, dehydrogenated isocyanate is used as a raw material, lithium aluminum hydride is added to undergo a reduction reaction to obtain intermediate 1, and then intermediate 1 is esterified with butyric anhydride to obtain intermediate 2; subsequently, chitosan is used as a raw material, and intermediate D is prepared through amino protection, bromination, azide, reduction and deprotection reactions, and intermediate 2 is mixed with intermediate D to obtain a composite additive.

[0012] Preferably, in S1, the amount ratio of dehydroabietic acid, tetrahydrofuran and lithium aluminum hydride is (19.08-22.34) g: (400-480) mL: (45.6-50.12) g, the low-temperature stirring temperature is -10~-14°C, the dropwise addition time is 2-3 h, the reaction time is 0.5-1 h, the weight fraction of the sodium hydroxide solution is 10wt%, and it is dried with anhydrous magnesium sulfate and the standing time is 10-12 h; the amount ratio of the intermediate 1 and butyric anhydride is (5-7.3) g: (40-55) mL, the heating and stirring reaction temperature is 50-55°C, the reaction time is 5-8 h, and it is washed with a sodium carbonate solution with a mass fraction of 10wt%.

[0013] Preferably, in S2, the dosage ratio of phthalic anhydride, N,N-dimethylformamide and chitosan is (20-24.4) g: (220-240) mL: (4.8-5.1) g, the heating reaction temperature is 110-130°C, and the reaction time is 8-10 h; the dosage ratio of intermediate A, N-methyl-2-pyrrolidone, N-bromosuccinimide and triphenylphosphine is (3-5) g: (310-330) mL: (20-24) g: (28.5-32.4) g, the low temperature environment temperature is 1-3°C, the heating reaction temperature is 80-90°C, and the reaction time is 2-4 h.

[0014] Preferably, in S3, the amount ratio of intermediate B, sodium azide and dimethyl sulfoxide is (2.4-3.2) g: (4.8-5.6) g: (260-310) mL, the temperature of the reaction is 80-85°C, and the reaction time is 4-6 h; the amount ratio of intermediate C, triphenylphosphine, N,N-dimethylformamide and hydrazine hydrate is (1-2.2) g: (4-6.2) g: (140-180) mL: (70-90) mL, the reaction time at room temperature is 16-20 h, and the temperature of the reaction is 90-100°C, and the reaction time is 6-8 h; in S4, the amount ratio of intermediate 2, intermediate D and distilled water is (2-5) g: (1-2.1) g: (4.5-6.2) mL.

[0015] Preferably, a method for preparing a chinensis promoter comprises the following steps: Step 1: Mixing sheep manure, nitrogen-phosphorus compound fertilizer and wheat bran to obtain a mixture; Step 2: Add the plant extract and the composite additive into the mixture, stir and mix, and obtain the sheepgrass promoter.

[0016] Preferably, a method for restoring grassland using a sheep grass promoter comprises the following steps: P1: Chop the underground part of Leymus chinensis into pieces, including roots, rhizomes and buds, into 1 cm segments to obtain pretreated Leymus chinensis; P2: spraying the pretreated chinensis with a chinensis promoter to obtain reproductive body segments; P3: Use a seeder to sow the reproductive body segments into the natural grassland at a depth of 2-4cm to repair the grassland.

[0017] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are: The prepared plant extract and composite additive are simultaneously added to the sheep grass promoter, which can effectively improve the growth of the sheep grass. The plant extract has the function of promoting plant growth, and can promote cell division and elongation by regulating the hormone level in the plant, thereby accelerating the growth of the sheep grass. The presence of hormones can jointly promote the growth of the sheep grass by regulating multiple physiological processes such as photosynthesis, respiration and material transport of the plant. The presence of the composite additive can effectively improve the stress resistance of the sheep grass, so that the sheep grass can still maintain a good growth state under an unfavorable environment, and can also enhance the plant's adaptability and response ability to adversity by regulating the signal transduction pathway in the plant. The presence of the composite additive can also improve the growth and development of the sheep grass root system, improve its absorption and transport ability of soil nutrients, make it better use of nutrient resources in the soil, and accelerate growth. DETAILED DESCRIPTION

[0018] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] Example 1: This example discloses a method for preparing a plant extract, comprising the following steps: Q1: Wash the Sargassum with clean seawater, dry the surface water with absorbent paper, cut and crush 75g of clean Sargassum, homogenize with a homogenizer, homogenize for 2min every 5min, for a total of 3 times, then add 225mL of distilled water, heat and extract at 38℃ for 4h, centrifuge at 12000rpm for 8min, collect the supernatant, and obtain the extract; Q2: Mix 1.5 mL of the extract with 16 mL of a 0.6 wt% cellulase solution, adjust the pH to 4.2, heat at 55°C for 60 min, heat at 100°C for 5 min to inactivate the enzyme, centrifuge at 8000 rpm for 12 min, collect the supernatant, and obtain a plant extract.

[0020] This embodiment discloses a method for preparing a composite additive, comprising the following steps: S1: 20.58g of dehydroabietic acid was added to a container containing 440mL of tetrahydrofuran, and 47.36g of lithium aluminum hydride was slowly added under low temperature stirring at -10°C, and the adding time was controlled to be 2h. After the addition was completed, the reaction was carried out for 0.5h. After the reaction was completed, the mixture was heated under reflux at 67°C for 1h. After the reaction was completed, 4.6mL of distilled water was added dropwise under low temperature, and then 4.6mL of 10wt% sodium hydroxide solution was added, and then 13.8mL of distilled water was added dropwise, and the mixture was dried with anhydrous magnesium sulfate, allowed to stand, filtered, and rotary evaporated to obtain intermediate 1; 6.15g of intermediate 1 was added to a container, and then 47.5mL of butyric anhydride was added, and the mixture was heated and stirred at 55°C for 8h. The mixture was washed with 10wt% sodium carbonate solution, separated, and rotary evaporated to obtain intermediate 2; S2: Add 22.2g of phthalic anhydride to a container containing 230mL of N,N-dimethylformamide, stir to dissolve, add 4.95g of chitosan, heat to 120℃ for 10h under nitrogen protection, cool down after the reaction, pour into ice water, centrifuge, wash, filter, and dry to obtain intermediate A; add 4g of intermediate A to a container containing 320mL of N-methyl-2-pyrrolidone, slowly add 22g of N-bromosuccinimide and 30.5g of triphenylphosphine at 3℃, heat to 90℃ for 4h, cool down after the reaction, place in anhydrous ethanol for precipitation, centrifuge, filter, and dry to obtain intermediate B; S3: 2.8 g of intermediate B, 5.2 g of sodium azide and 280 mL of dimethyl sulfoxide were added to a container, and the temperature was raised to 85°C for reaction for 4 h under nitrogen protection. After the reaction was completed, the temperature was lowered, and the mixture was poured into ethanol for precipitation. The mixture was centrifuged, filtered and dried to obtain intermediate C. 1.6 g of intermediate C, 5.6 g of triphenylphosphine and 160 mL of N,N-dimethylformamide were added to a container, and the temperature was raised to 100°C for reaction for 8 h. The mixture was lowered, poured into ethanol for precipitation, centrifuged, filtered and dried to obtain intermediate D. S4: 3.5 g of the intermediate 2 and 1.5 g of the intermediate D were added sequentially into a container containing 5.3 mL of distilled water, and stirred for reaction to obtain a composite additive.

[0021] The present embodiment discloses a sheep grass promoter, which is composed of the following ingredients in parts by weight: 59 parts of sheep manure, 39 parts of wheat bran, 4 parts of plant extract, 6 parts of composite additives, and 80 parts of nitrogen-phosphorus compound fertilizer.

[0022] This embodiment discloses a method for preparing a leymus chinensis promoter, comprising the following steps: Step 1: Mixing sheep manure, nitrogen-phosphorus compound fertilizer and wheat bran to obtain a mixture; Step 2: Add the plant extract and the composite additive into the mixture, stir and mix, and obtain the sheepgrass promoter.

[0023] This embodiment discloses a method for repairing grassland using a sheep grass promoter, comprising the following steps: P1: Chop the underground part of Leymus chinensis into pieces, including roots, rhizomes and buds, into 1 cm segments to obtain pretreated Leymus chinensis; P2: spraying the pretreated chinensis with a chinensis promoter to obtain reproductive body segments; P3: Use a seeder to sow the reproductive body segments into the natural grassland at a depth of 3 cm to repair the grassland.

[0024] Example 2: This example discloses a method for preparing a plant extract, comprising the following steps: Q1: Wash the Sargassum with clean seawater, dry the surface water with absorbent paper, cut and crush 50g of clean Sargassum, homogenize with a homogenizer, homogenize for 2min every 5min, for a total of 3 times, then add 300mL of distilled water, heat and extract at 38℃ for 4h, centrifuge at 12000rpm for 8min, collect the supernatant, and obtain the extract; Q2: Mix 1 mL of the extract with 12 mL of a 0.6 wt% cellulase solution, adjust the pH to 4.2, heat at 55°C for 60 min, heat at 100°C for 5 min to inactivate the enzyme, centrifuge at 8000 rpm for 12 min, collect the supernatant, and obtain a plant extract.

[0025] This embodiment discloses a method for preparing a composite additive, comprising the following steps: S1: 19.08 g of dehydroabietic acid was added to a container containing 480 mL of tetrahydrofuran, and 45.6 g of lithium aluminum hydride was slowly added under low temperature stirring at -10°C, and the adding time was controlled to be 2 h. After the addition was completed, the reaction was carried out for 0.5 h. After the reaction was completed, the mixture was heated under reflux at 67°C for 1 h. After the reaction was completed, 4.6 mL of distilled water was added dropwise under low temperature conditions, and then 4.6 mL of 10 wt% sodium hydroxide solution was added, and then 13.8 mL of distilled water was added dropwise, and the mixture was dried with anhydrous magnesium sulfate, allowed to stand, filtered, and rotary evaporated to obtain intermediate 1; 5 g of intermediate 1 was added to a container, and then 40 mL of butyric anhydride was added, and the mixture was heated and stirred at 55°C for 8 h. The mixture was washed with 10 wt% sodium carbonate solution, separated, and rotary evaporated to obtain intermediate 2; S2: Add 20g of phthalic anhydride to a container containing 240mL of N,N-dimethylformamide, stir to dissolve, add 4.8g of chitosan, heat to react at 120°C for 10h under nitrogen protection, cool down after the reaction, pour into ice water, centrifuge, wash, filter, and dry to obtain intermediate A; add 3g of intermediate A to a container containing 310mL of N-methyl-2-pyrrolidone, slowly add 24g of N-bromosuccinimide and 28.5g of triphenylphosphine at 3°C, heat to react at 90°C for 4h, cool down after the reaction, place in anhydrous ethanol for precipitation, centrifuge, filter, and dry to obtain intermediate B; S3: 2.4 g of intermediate B, 4.8 g of sodium azide and 260 mL of dimethyl sulfoxide were added to a container, and the temperature was raised to 85°C for reaction for 4 h under nitrogen protection. After the reaction was completed, the temperature was lowered, and the mixture was poured into ethanol for precipitation. The mixture was centrifuged, filtered and dried to obtain intermediate C. 2.2 g of intermediate C, 6.2 g of triphenylphosphine and 140 mL of N,N-dimethylformamide were added to a container, and the temperature was raised to 100°C for reaction for 8 h. The mixture was lowered, poured into ethanol for precipitation, centrifuged, filtered and dried to obtain intermediate D. S4: 2 g of the intermediate 2 and 1 g of the intermediate D were added sequentially into a container containing 4.5 mL of distilled water, and stirred for reaction to obtain a composite additive.

[0026] The present embodiment discloses a sheep grass promoter, which is composed of the following ingredients in parts by weight: 52 parts of sheep manure, 32 parts of wheat bran, 6 parts of plant extract, 2 parts of compound additives, and 70 parts of nitrogen-phosphorus compound fertilizer.

[0027] This embodiment discloses a method for preparing a leymus chinensis promoter, comprising the following steps: Step 1: Mixing sheep manure, nitrogen-phosphorus compound fertilizer and wheat bran to obtain a mixture; Step 2: Add the plant extract and the composite additive into the mixture, stir and mix, and obtain the sheepgrass promoter.

[0028] This embodiment discloses a method for repairing grassland using a sheep grass promoter, comprising the following steps: P1: Chop the underground part of Leymus chinensis into pieces, including roots, rhizomes and buds, into 1 cm segments to obtain pretreated Leymus chinensis; P2: spraying the pretreated chinensis with a chinensis promoter to obtain reproductive body segments; P3: Use a seeder to sow the reproductive body segments into the natural grassland at a depth of 3 cm to repair the grassland.

[0029] Example 3: This example discloses a method for preparing a plant extract, comprising the following steps: Q1: Wash the Sargassum with clean seawater, dry the surface water with absorbent paper, cut and crush 100g of clean Sargassum, homogenize with a homogenizer, homogenize for 2min every 5min, for a total of 3 times, then add 150mL of distilled water, heat and extract at 38℃ for 4h, centrifuge at 12000rpm for 8min, collect the supernatant, and obtain the extract; Q2: Mix 2 mL of the extract with 20 mL of a 0.6 wt% cellulase solution, adjust the pH to 4.2, heat at 55°C for 60 min, heat at 100°C for 5 min to inactivate the enzyme, centrifuge at 8000 rpm for 12 min, collect the supernatant, and obtain a plant extract.

[0030] This embodiment discloses a method for preparing a composite additive, comprising the following steps: S1: Add 22.34g of dehydroabietic acid to a container containing 400mL of tetrahydrofuran, slowly add 50.12g of lithium aluminum hydride under low temperature stirring at -10°C, control the adding time to be 2h, react for 0.5h after the addition is completed, heat under reflux at 67°C for 1h after the reaction is completed, drop 4.6mL of distilled water under low temperature, then add 4.6mL of 10wt% sodium hydroxide solution, then drop 13.8mL of distilled water, dry with anhydrous magnesium sulfate, let stand, filter with suction, and rotary evaporate to obtain intermediate 1; add 7.3g of intermediate 1 to a container, then add 55mL of butyric anhydride, heat and stir at 55°C for 8h, wash with 10wt% sodium carbonate solution, separate, and rotary evaporate to obtain intermediate 2; S2: Add 24.4g of phthalic anhydride to a container containing 220mL of N,N-dimethylformamide, stir to dissolve, add 5.1g of chitosan, heat to 120℃ for 10h under nitrogen protection, cool down after the reaction, pour into ice water, centrifuge, wash, filter, and dry to obtain intermediate A; add 5g of intermediate A to a container containing 330mL of N-methyl-2-pyrrolidone, slowly add 20g of N-bromosuccinimide and 32.4g of triphenylphosphine at 3℃, heat to 90℃ for 4h, cool down after the reaction, place in anhydrous ethanol for precipitation, centrifuge, filter, and dry to obtain intermediate B; S3: 3.2 g of intermediate B, 5.6 g of sodium azide and 310 mL of dimethyl sulfoxide were added to a container, and the temperature was raised to 85°C for reaction for 4 h under nitrogen protection. After the reaction was completed, the temperature was lowered, and the mixture was poured into ethanol for precipitation. The mixture was centrifuged, filtered and dried to obtain intermediate C. 1 g of intermediate C, 4 g of triphenylphosphine and 180 mL of N,N-dimethylformamide were added to a container, and the temperature was raised to 100°C for reaction for 8 h under nitrogen protection. The mixture was then lowered, poured into ethanol for precipitation, centrifuged, filtered and dried to obtain intermediate D. S4: 5 g of the intermediate 2 and 2.1 g of the intermediate D were added sequentially into a container containing 6.2 mL of distilled water, and stirred for reaction to obtain a composite additive.

[0031] The present embodiment discloses a sheep grass promoter, which is composed of the following ingredients in parts by weight: 66 parts of sheep manure, 46 parts of wheat bran, 2 parts of plant extract, 10 parts of compound additives, and 90 parts of nitrogen-phosphorus compound fertilizer.

[0032] This embodiment discloses a method for preparing a leymus chinensis promoter, comprising the following steps: Step 1: Mixing sheep manure, nitrogen-phosphorus compound fertilizer and wheat bran to obtain a mixture; Step 2: Add the plant extract and the composite additive into the mixture, stir and mix, and obtain the sheepgrass promoter.

[0033] This embodiment discloses a method for repairing grassland using a sheep grass promoter, comprising the following steps: P1: Chop the underground part of Leymus chinensis into pieces, including roots, rhizomes and buds, into 1 cm segments to obtain pretreated Leymus chinensis; P2: spraying the pretreated chinensis with a chinensis promoter to obtain reproductive body segments; P3: Use a seeder to sow the reproductive body segments into the natural grassland at a depth of 3 cm to repair the grassland.

[0034] Example 4: This example discloses a method for preparing a plant extract, comprising the following steps: Q1: Wash the Sargassum with clean seawater, dry the surface water with absorbent paper, cut and crush 60g of clean Sargassum, homogenize with a homogenizer, homogenize for 2min every 5min, for a total of 3 times, then add 200mL of distilled water, heat and extract at 38℃ for 4h, centrifuge at 12000rpm for 8min, collect the supernatant, and obtain the extract; Q2: Mix 1.1 mL of the extract with 14 mL of a 0.6 wt% cellulase solution, adjust the pH to 4.2, heat at 55°C for 60 min, heat at 100°C for 5 min to inactivate the enzyme, centrifuge at 8000 rpm for 12 min, collect the supernatant, and obtain a plant extract.

[0035] This embodiment discloses a method for preparing a composite additive, comprising the following steps: S1: 19.87 g of dehydroabietic acid was added to a container containing 420 mL of tetrahydrofuran, and 46.28 g of lithium aluminum hydride was slowly added under low temperature stirring at -10 ° C, and the adding time was controlled to be 2 h. After the addition was completed, the reaction was carried out for 0.5 h. After the reaction was completed, the mixture was heated under reflux at 67 ° C for 1 h. After the reaction was completed, 4.6 mL of distilled water was added dropwise under low temperature conditions, and then 4.6 mL of 10 wt% sodium hydroxide solution was added, and then 13.8 mL of distilled water was added dropwise, and the mixture was dried with anhydrous magnesium sulfate, allowed to stand, filtered, and rotary evaporated to obtain intermediate 1; 6.5 g of intermediate 1 was added to a container, and then 42 mL of butyric anhydride was added, heated and stirred at 55 ° C for 8 h, washed with 10 wt% sodium carbonate solution, separated, and rotary evaporated to obtain intermediate 2; S2: Add 21.3g of phthalic anhydride to a container containing 225mL of N,N-dimethylformamide, stir to dissolve, add 4.9g of chitosan, heat to 120℃ for 10h under nitrogen protection, cool down after the reaction, pour into ice water, centrifuge, wash, filter, and dry to obtain intermediate A; add 3.5g of intermediate A to a container containing 315mL of N-methyl-2-pyrrolidone, slowly add 21g of N-bromosuccinimide and 29.3g of triphenylphosphine at 3℃, heat to 90℃ for 4h, cool down after the reaction, place in anhydrous ethanol for precipitation, centrifuge, filter, and dry to obtain intermediate B; S3: 2.6 g of intermediate B, 4.9 g of sodium azide and 270 mL of dimethyl sulfoxide were added to a container, and the temperature was raised to 85°C for reaction for 4 h under nitrogen protection. After the reaction was completed, the temperature was lowered, and the mixture was poured into ethanol for precipitation. The mixture was centrifuged, filtered and dried to obtain intermediate C. 1.4 g of intermediate C, 4.8 g of triphenylphosphine and 150 mL of N,N-dimethylformamide were added to a container, and the temperature was raised to 100°C for reaction for 8 h. The mixture was lowered, poured into ethanol for precipitation, centrifuged, filtered and dried to obtain intermediate D. S4: 3 g of the intermediate 2 and 1.7 g of the intermediate D were added sequentially into a container containing 4.8 mL of distilled water, and stirred for reaction to obtain a composite additive.

[0036] The present embodiment discloses a sheep grass promoter, which is composed of the following ingredients in parts by weight: 54 parts of sheep manure, 36 parts of wheat bran, 3 parts of plant extract, 4 parts of compound additives, and 75 parts of nitrogen-phosphorus compound fertilizer.

[0037] This embodiment discloses a method for preparing a leymus chinensis promoter, comprising the following steps: Step 1: Mixing sheep manure, nitrogen-phosphorus compound fertilizer and wheat bran to obtain a mixture; Step 2: Add the plant extract and the composite additive into the mixture, stir and mix, and obtain the sheepgrass promoter.

[0038] This embodiment discloses a method for repairing grassland using a sheep grass promoter, comprising the following steps: P1: Chop the underground part of Leymus chinensis into pieces, including roots, rhizomes and buds, into 1 cm segments to obtain pretreated Leymus chinensis; P2: spraying the pretreated chinensis with a chinensis promoter to obtain reproductive body segments; P3: Use a seeder to sow the reproductive body segments into the natural grassland at a depth of 3 cm to repair the grassland.

[0039] Example 5: This example discloses a method for preparing a plant extract, comprising the following steps: Q1: Wash the Sargassum with clean seawater, dry the surface water with absorbent paper, cut and crush 90g of clean Sargassum, homogenize with a homogenizer, homogenize for 2min every 5min, for a total of 3 times, then add 250mL of distilled water, heat and extract at 38℃ for 4h, centrifuge at 12000rpm for 8min, collect the supernatant, and obtain the extract; Q2: Mix 1.7 mL of the extract with 19 mL of a 0.6 wt% cellulase solution, adjust the pH to 4.2, heat at 55°C for 60 min, heat at 100°C for 5 min to inactivate the enzyme, centrifuge at 8000 rpm for 12 min, collect the supernatant, and obtain a plant extract.

[0040] This embodiment discloses a method for preparing a composite additive, comprising the following steps: S1: Add 21.12g of dehydroabietic acid to a container containing 460mL of tetrahydrofuran, slowly add 49.17g of lithium aluminum hydride under low temperature stirring at -10℃, control the adding time to be 2h, react for 0.5h after the addition is completed, heat under reflux at 67℃ for 1h after the reaction is completed, drop 4.6mL of distilled water under low temperature, then add 4.6mL of 10wt% sodium hydroxide solution, then drop 13.8mL of distilled water, dry with anhydrous magnesium sulfate, let stand, filter with suction, and rotary evaporate to obtain intermediate 1; add 7.1g of intermediate 1 to a container, then add 52mL of butyric anhydride, heat and stir at 55℃ for 8h, wash with 10wt% sodium carbonate solution, separate, and rotary evaporate to obtain intermediate 2; S2: Add 23.8g of phthalic anhydride to a container containing 235mL of N,N-dimethylformamide, stir to dissolve, add 5g of chitosan, heat to 120℃ for 10h under nitrogen protection, cool down after the reaction, pour into ice water, centrifuge, wash, filter, and dry to obtain intermediate A; add 4.5g of intermediate A to a container containing 325mL of N-methyl-2-pyrrolidone, slowly add 23g of N-bromosuccinimide and 31.7g of triphenylphosphine at 3℃, heat to 90℃ for 4h, cool down after the reaction, place in anhydrous ethanol for precipitation, centrifuge, filter, and dry to obtain intermediate B; S3: 3 g of intermediate B, 5.4 g of sodium azide and 300 mL of dimethyl sulfoxide were added to a container, and the temperature was raised to 85°C for reaction for 4 h under nitrogen protection. After the reaction was completed, the temperature was lowered, and the mixture was poured into ethanol for precipitation. The mixture was centrifuged, filtered and dried to obtain intermediate C. 2 g of intermediate C, 5.8 g of triphenylphosphine and 170 mL of N,N-dimethylformamide were added to a container, and the temperature was raised to 100°C for reaction for 8 h. The mixture was lowered, poured into ethanol for precipitation, centrifuged, filtered and dried to obtain intermediate D. S4: 4 g of the intermediate 2 and 1.9 g of the intermediate D were added sequentially into a container containing 5.9 mL of distilled water, and stirred for reaction to obtain a composite additive.

[0041] The present embodiment discloses a sheep grass promoter, which is composed of the following ingredients in parts by weight: 63 parts of sheep manure, 42 parts of wheat bran, 5 parts of plant extract, 8 parts of composite additives, and 85 parts of nitrogen-phosphorus compound fertilizer.

[0042] This embodiment discloses a method for preparing a leymus chinensis promoter, comprising the following steps: Step 1: Mixing sheep manure, nitrogen-phosphorus compound fertilizer and wheat bran to obtain a mixture; Step 2: Add the plant extract and the composite additive into the mixture, stir and mix, and obtain the sheepgrass promoter.

[0043] This embodiment discloses a method for repairing grassland using a sheep grass promoter, comprising the following steps: P1: Chop the underground part of Leymus chinensis into pieces, including roots, rhizomes and buds, into 1 cm segments to obtain pretreated Leymus chinensis; P2: spraying the pretreated chinensis with a chinensis promoter to obtain reproductive body segments; P3: Use a seeder to sow the reproductive body segments into the natural grassland at a depth of 3 cm to repair the grassland.

[0044] Comparative Example 1: Compared with Example 1, in the preparation process of the sheep fescue promoter in Comparative Example 1, no plant extractant is added, and other conditions remain unchanged.

[0045] Comparative Example 2: Compared with Example 1, in the preparation process of the sheepgrass promoter in Comparative Example 2, no composite additive is added, and other conditions remain unchanged.

[0046] Experimental Example: The samples prepared according to Examples 1-5 and Comparative Examples 1-2 were applied to the planting process of Leymus chinensis, and a blank control group (no Leymus chinensis promoter was applied during the planting process) was set up to test the density and biomass of Leymus chinensis. The test results are shown in Table 1:

[0047] It can be seen from the test results in Table 1 that the leopard grass promoter prepared by Examples 1-5 of the present invention can effectively increase the density and biomass of leopard grass. It can be seen from the comparison between Comparative Example 1 and Examples 1-5 that the addition of plant extracts can effectively increase the density and biomass of leopard grass; it can be seen from the comparison between Comparative Example 2 and Examples 1-5 that the addition of composite additives can effectively increase the density and biomass of leopard grass.

[0048] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

[0049] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to only specific implementation methods. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A leymus chinensis promoter, characterized in that: The invention comprises the following ingredients in parts by weight: 52-66 parts of sheep manure, 32-46 parts of wheat bran, 2-6 parts of plant extractant, 2-10 parts of composite additive and 70-90 parts of nitrogen-phosphorus compound fertilizer, wherein the plant extractant is obtained from Sargassum by combining extraction and enzymolysis, and the composite additive is prepared from dehydroabietic acid, lithium aluminum hydride, phthalic anhydride, N-bromosuccinimide, sodium azide and triphenylphosphine.

2. A chinensis promoter according to claim 1, characterized in that: The preparation method of the plant extract comprises the following steps: Q1: Wash the Sargassum with clean seawater, absorb the moisture on its surface with absorbent paper, cut and crush the clean Sargassum, homogenize it with a homogenizer, then add distilled water, heat and extract, centrifuge, collect the supernatant, and obtain the extract; Q2: Mix the extract with the cellulase solution, adjust the pH, heat for enzymolysis, increase the temperature to inactivate the enzyme, centrifuge, collect the supernatant, and obtain the plant extract.

3. A chinensis promoter according to claim 2, characterized in that: In the Q1, the usage ratio of clean Sargassum and distilled water is (50-100) g: (150-300) mL. During the homogenization process, the homogenization is performed for 2 minutes every 5 minutes, for a total of 3-5 times. The heating and extraction temperature is 35-38° C., the time is 2-4 hours, the centrifugal speed is 10000-12000 rpm, and the centrifugal time is 5-8 minutes.

4. A chinensis promoter according to claim 2, characterized in that: In the Q2, the volume ratio of the extract to the cellulase solution is (1-2): (12-20), the mass fraction of the cellulase solution is 0.6wt%, the pH is adjusted to 4-5, the heating enzymatic hydrolysis temperature is 50-55°C, the enzymatic hydrolysis time is 60-75min, the heating enzyme inactivation temperature is 95-100°C, the time is 3-5min, the centrifugal speed is 8000-12000rpm, and the centrifugal time is 10-15min.

5. The ginseng grass promoter according to claim 1, characterized in that: The preparation method of the composite additive comprises the following steps: S1: adding dehydroabietic acid to a container containing tetrahydrofuran, slowly adding lithium aluminum hydride under low temperature stirring conditions, controlling the adding time, reacting after the addition is completed, heating under reflux reaction after the reaction is completed, adding distilled water dropwise under low temperature conditions, then adding sodium hydroxide solution, then adding distilled water dropwise, drying, standing, suction filtering, rotary evaporation, to obtain intermediate 1; adding intermediate 1 to a container, then adding butyric anhydride, heating and stirring to react, washing, separating the liquids, rotary evaporation, to obtain intermediate 2; S2: Add phthalic anhydride to a container filled with N,N-dimethylformamide, stir to dissolve, add chitosan, heat to react under nitrogen protection, cool after the reaction, pour into ice water, centrifuge, wash, filter, and dry to obtain intermediate A; add intermediate A to a container filled with N-methyl-2-pyrrolidone, slowly add N-bromosuccinimide and triphenylphosphine under low temperature, heat to react, cool after the reaction, place in anhydrous ethanol for precipitation, centrifuge, filter, and dry to obtain intermediate B; S3: Add intermediate B, sodium azide and dimethyl sulfoxide to a container, heat the reaction under nitrogen protection, cool down after the reaction, pour into ethanol for precipitation, centrifuge, filter, and dry to obtain intermediate C; add intermediate C, triphenylphosphine and N,N-dimethylformamide to a container, react at room temperature under nitrogen protection, then add hydrazine hydrate, heat the reaction, cool down, pour into ethanol for precipitation, centrifuge, filter, and dry to obtain intermediate D; S4: adding the intermediate 2 and intermediate D obtained into a container filled with distilled water in sequence, stirring and reacting, and obtaining a composite additive.

6. A chinensis promoter according to claim 5, characterized in that: In the S1, the amount ratio of dehydroabietic acid, tetrahydrofuran and lithium aluminum hydride is (19.08-22.34) g: (400-480) mL: (45.6-50.12) g, the low-temperature stirring temperature is -10~-14°C, the dropwise addition time is 2-3 h, the reaction time is 0.5-1 h, the weight portion of the sodium hydroxide solution is 10wt%, and it is dried with anhydrous magnesium sulfate and left to stand for 10-12 h; the amount ratio of the intermediate 1 and butyric anhydride is (5-7.3) g: (40-55) mL, the heating and stirring reaction temperature is 50-55°C, the reaction time is 5-8 h, and it is washed with a sodium carbonate solution with a mass fraction of 10wt%.

7. The ginseng grass promoter according to claim 5, characterized in that: In the S2, the dosage ratio of phthalic anhydride, N,N-dimethylformamide and chitosan is (20-24.4) g: (220-240) mL: (4.8-5.1) g, the heating reaction temperature is 110-130°C, and the reaction time is 8-10 h; the dosage ratio of intermediate A, N-methyl-2-pyrrolidone, N-bromosuccinimide and triphenylphosphine is (3-5) g: (310-330) mL: (20-24) g: (28.5-32.4) g, the low temperature environment temperature is 1-3°C, the heating reaction temperature is 80-90°C, and the reaction time is 2-4 h.

8. The ginseng grass promoter according to claim 5, characterized in that: In the S3, the amount ratio of intermediate B, sodium azide and dimethyl sulfoxide is (2.4-3.2) g: (4.8-5.6) g: (260-310) mL, the temperature of the reaction is 80-85°C, and the reaction time is 4-6 h; the amount ratio of intermediate C, triphenylphosphine, N,N-dimethylformamide and hydrazine hydrate is (1-2.2) g: (4-6.2) g: (140-180) mL: (70-90) mL, the reaction time at room temperature is 16-20 h, and the temperature of the reaction is 90-100°C, and the reaction time is 6-8 h; in the S4, the amount ratio of intermediate 2, intermediate D and distilled water is (2-5) g: (1-2.1) g: (4.5-6.2) mL.

9. The method for preparing a chinensis promoter according to claims 1-8, characterized in that: The following steps are involved: Step 1: Mixing sheep manure, nitrogen-phosphorus compound fertilizer and wheat bran to obtain a mixture; Step 2: Add the plant extract and the composite additive into the mixture, stir and mix, and obtain the sheepgrass promoter.

10. A method for restoring grassland using a chinensis promoter, characterized in that: The following steps are involved: P1: Chop the underground part of Leymus chinensis into pieces, including roots, rhizomes and buds, into 1 cm segments to obtain pretreated Leymus chinensis; P2: spraying the pretreated chinensis with a chinensis promoter to obtain reproductive body segments; P3: Use a seeder to sow the reproductive body segments into the natural grassland at a depth of 2-4cm to repair the grassland.

Images