Nucleic acid detection and analysis device with uniform mixing structure

Abstract

The invention discloses a nucleic acid detection and analysis device with a uniform mixing structure, and relates to the technical field of nucleic acid detection and analysis devices.The nucleic acid detection and analysis device comprises a detection box, a placement table is fixedly mounted at the bottom in the detection box, driving rods are rotationally connected to the periphery of the placement table, a pair of second gears is fixed to each driving rod, and the second gears are rotationally connected to the placement table; the multiple second gears are sleeved with a chain belt, multiple magnetic attraction bases are fixedly installed on the inner ring of the chain belt, one side of each magnetic attraction base is fixedly connected with a pair of chipping pieces, a butt joint table is fixedly installed at the top of the containing table, and inserting rods are detachably connected to the two sides of the top of the butt joint table. According to the nucleic acid detection and analysis device with the uniform mixing structure, the kit and the top position pieces are detachable, so that the sampling needle and the magnetic head seat are disinfected at the same time in the result detection process of the device, the two operations have synchronism, and the accuracy of the detection result is improved. The number of samples detected by the device in a short time is increased, and the sample detection time is shortened.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection and analysis devices, and in particular to a nucleic acid detection and analysis device with a mixing structure. Background Art

[0002] After the existing nucleic acid is sampled, the entire device is mixed and then placed in a detection instrument for detection. First, when sampling, the existing device generally carries multiple DNA fragments as analysis objects on a flat substrate, and identifies the base sequences of the multiple carried DNA fragments in parallel. The invention patent with Chinese patent authorization number CN115521863B proposes a technical solution, which separates the pipette and the soft rubber sleeve, then drives the pipette down so that the piston rod sucks the sample into the pipette, then drives the device to the top of the reagent box, and finally lowers the piston rod. During the process, the sample inside the pipette is squeezed into the reagent box for reaction, and finally detected by the optical detector, and the information is transmitted to the host computer. Although nucleic acid detection and analysis can be performed, because only a single translation unit is used to perform translational action in one direction, for example, when processing multiple batches of samples, the inside of the pipette needs to be disinfected multiple times before use, and the required movement amount increases. Due to the connecting structure such as the piston rod, the disinfection time inside the pipette is relatively long, the operations performed by the equipment are relatively cumbersome, and the detection efficiency is relatively low. For this reason, we propose a nucleic acid detection and analysis device with a mixing structure. Summary of the invention

[0003] The purpose of the present invention is to provide a nucleic acid detection and analysis device with a mixing structure to solve the problems raised in the above-mentioned background technology.

[0004] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a nucleic acid detection and analysis device with a mixing structure, comprising a detection box, a placing table is fixedly installed at the bottom of the detection box, and driving rods are rotatably connected to the placing table on all sides, a pair of second gears are fixed on each of the driving rods, and a chain belt is sleeved on the plurality of second gears, and a plurality of magnetic seats are fixedly installed on the inner ring of the chain belt, and a pair of chippers are fixedly connected to one side of each of the magnetic seats, a docking table is fixedly installed on the top of the placing table, and insertion rods are detachably connected to both sides of the top of the docking table, and a top position piece is fixedly connected to the top of each insertion rod, a drainage machine is fixedly connected to the middle part of one side of the outer side of the detection box, a spray pressure chamber is fixedly arranged on one side of the detection box, and the spray pressure chamber and the drainage machine are fixedly connected, one of the top position pieces is located inside the spray pressure chamber, a reagent box is detachably connected to one side of the docking table, a first cylinder is suspended at the top of the detection box, and a detector is fixedly connected to the bottom of the first cylinder; A mixing structure for mixing the sampling tube is fixedly connected to one side of the placement table. After the mixing structure contacts the chip, the liquid to be tested is transferred to the inside of the reagent box for testing through the movement of the chain belt.

[0005] Preferably, the mixing structure includes a plurality of transmission rods rotatably connected to the top of the detection box, a gasket is fixedly connected to the bottom of each transmission rod, a first gear is fixedly connected to the top of each transmission rod, a motor is fixedly connected to the top of the detection box, a belt is sleeved on the output shaft of the motor, a rotating rod is sleeved on one end of the belt, one end of the rotating rod is suspended inside the detection box by passing through the detection box, a center block is slidably connected to the bottom of the rotating rod, a mixing disk is slidably connected to the outside of the center block, a first spring is fixedly connected to the inner bottom of the rotating rod, the first spring and the center block are fixedly connected, a driving gear is fixedly installed on the top of the rotating rod, and the driving gear is meshed with each of the first gears.

[0006] Preferably, the middle of the mixing plate is sunken downward to form a placement area, the placement area is annular in shape, and a plurality of puncture holes are opened at the bottom, and the placement area is slidably connected to the sampling tube.

[0007] Preferably, a base is fixedly connected to one side of the top of the placement table, a second cylinder is fixedly connected to the middle of the base, and a quantitative sampling device for extracting the liquid to be tested in the sampling tube is installed on the top of the second cylinder.

[0008] Preferably, the quantitative sampling device includes a connecting tube fixed on the top of the second cylinder, a magnetic suction cup is installed on the top of the connecting tube, the top of the magnetic suction cup is connected to a plurality of magnetic head seats by magnetic attraction, and a sampling needle is fixedly connected to the bottom of each magnetic head seat, and each sampling needle corresponds to the bottom of the puncture hole.

[0009] Preferably, the quantitative sampling device also includes an insert arranged on the top of the outer ring of the connecting tube, the inner ring of the magnetic suction cup is provided with a groove corresponding to the insert, a second spring is fixedly connected to the inside of the connecting tube, a lifting plate is fixedly connected to the top of the second spring, and the lifting plate extends toward the bottom of the magnetic suction cup and contacts the bottom of the magnetic suction cup.

[0010] Preferably, each of the magnetic head seats is provided with a discharge cavity, and a material control ball is movably connected inside the discharge cavity. When the chip contacts the bottom of the magnetic head seat, the magnetic head seat and the magnetic seat are attracted together by magnetism. When the top piece contacts the material control ball, the material control ball is located inside the discharge cavity and moves.

[0011] Preferably, each of the discharge cavities is communicated with the sampling needle, and has an inner top side wall extending downward to form a shape that is wide at the top and narrow at the bottom, and the bottom of the discharge cavity is consistent with the control ball.

[0012] Preferably, the material control ball is made of metal, and its surface is polished.

[0013] Preferably, one end of the docking station is detachably connected to a collection bin, and the top of the collection bin is in communication with the bottom of the spray chamber.

[0014] Compared with the prior art, the present invention has the following beneficial effects: When the magnetic seat of the present invention is displaced to the top of the top piece, the chip passes over the side wall of the top piece, and the control ball contacts the top piece, so that the control ball rolls above the top piece. At the same time, the top piece allows the control ball to push up, opening the discharge chamber inside the magnetic head seat, so that the liquid to be tested drips from the inside of the discharge chamber along the top piece into the reagent box; when the control ball contacts the top piece again, the disinfectant enters the sampling needle and the inside of the discharge chamber to thoroughly disinfect them, and the disinfected disinfectant flows into the collection bin, which can be poured out manually after it is full. The reagent box and the top piece are detachable, so that the device can disinfect the sampling needle and the magnetic head seat at the same time during the detection of the results. The two operations are synchronized, which increases the number of samples detected by the device in a short time and reduces the detection time of the samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the structure of the present invention as a whole in an open state; Figure 3 For the present invention Figure 2 A schematic diagram of the enlarged structure at A in the middle; Figure 4 It is a schematic diagram of the structure of the mixing structure and the quantitative sampling device of the present invention; Figure 5 For the present invention Figure 4 A schematic diagram of the enlarged structure at B in the middle; Figure 6 It is a schematic diagram of the structure of the kit of the present invention; Figure 7 For the present invention Figure 6 The enlarged structural diagram at C in FIG. Figure 8 It is a schematic diagram of the structure of the drainage machine and the spray chamber of the present invention; Fig. 9 It is a schematic diagram of the structure of the magnetic head seat and the material control ball of the present invention.

[0016] In the figure: 1-test box; 2-drainage machine; 3-motor; 4-belt; 5-first cylinder; 501-detector; 6-placing table; 7-docking table; 8-first gear; 9-chain belt; 10-base; 11-second gear; 12-second cylinder; 1201-connecting tube; 13-transmission rod; 14-gasket; 15-rotating rod; 16-mixing disk; 17-first spring; 18-center block; 19-magnetic suction disk; 20-second spring; 21-lifting plate; 22-magnetic head seat; 2201-discharging chamber; 23-sampling needle; 24-material control ball; 25-collecting bin; 26-spraying chamber; 27-test kit; 28-penetrating rod; 29-top plate; 30-magnetic suction seat; 31-chipping. DETAILED DESCRIPTION

[0017] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] See also Figure 1-9The present invention provides a technical solution: a nucleic acid detection and analysis device with a mixing structure, comprising a detection box 1, a placement table 6 is fixedly installed at the bottom of the detection box 1, and the placement table 6 is rotatably connected to the driving rods on all sides, and a pair of second gears 11 are fixed on each driving rod, and a chain belt 9 is sleeved on the plurality of second gears 11, and a plurality of magnetic seats 30 are fixedly installed on the inner circle of the chain belt 9, and a pair of chippers 31 are fixedly connected to one side of each magnetic seat 30, and a docking table 7 is fixedly installed on the top of the placement table 6, and both sides of the top of the docking table 7 can be The detection box 1 is detachably connected with a penetration rod 28, and each penetration rod 28 is fixedly connected with a top piece 29 on the top. The middle part of the outer side of the detection box 1 is fixedly connected with a drainage machine 2. A spray pressure chamber 26 is fixedly arranged on one side of the detection box 1. The spray pressure chamber 26 and the drainage machine 2 are fixedly connected. One of the top pieces 29 is located inside the spray pressure chamber 26. A reagent box 27 is detachably connected to one side of the docking station 7. A first cylinder 5 is suspended on the top of the detection box 1, and a detector 501 is fixedly connected to the bottom of the first cylinder 5. A reagent box 27 is fixedly connected to one side of the placement table 6. The mixing structure is used to mix the sample tube, and the mixing structure contacts the chip 31 and then moves the chain belt 9 to transfer the test liquid to the inside of the reagent box 27 for detection; the mixing structure includes a plurality of transmission rods 13 rotatably connected to the top of the detection box 1, each transmission rod 13 is fixedly connected to a gasket 14 at the bottom, and each transmission rod 13 is fixedly connected to a first gear 8 at the top, and a motor 3 is fixedly connected to the top of the detection box 1, and a belt 4 is sleeved on the output shaft of the motor 3, and a rotating rod 15 is sleeved on one end of the belt 4, and a rotating rod 15 is sleeved on one end. The detection box 1 is suspended inside the detection box 1 by passing through the detection box 1. The bottom of the rotating rod 15 is slidably connected to the center block 18, and the outside of the center block 18 is slidably connected to the mixing disk 16. The bottom of the rotating rod 15 is fixedly connected to the first spring 17, and the first spring 17 and the center block 18 are fixedly connected. A driving gear is fixedly installed on the top of the rotating rod 15, and the driving gear and each first gear 8 are meshed; the middle part of the mixing disk 16 is recessed downward to form a placement area, and the placement area is annular, and a plurality of puncture holes are opened at the bottom, and the placement area and the sampling tube are slidably connected.

[0019] First, samples are collected manually outside the detection box 1, and the collected samples are packaged and stored through the sampling tube driven by the rubber seal. When the nucleic acid of the internal components of the sampling tube needs to be detected, the sampling tubes in batches are taken manually again and turned upside down and manually placed on the top of the mixing disk 16. During the contact between the rubber seal and the top of the mixing disk 16, the first spring 17 is stretched and the middle block 18 descends. During the downward working process of the first spring 17, the top of the sampling tube contacts the gasket 14, so that each sampling tube can be stabilized between the gasket 14 and the mixing disk 16, and the movable rod of the second cylinder 12 is driven to rise. When the second cylinder 12 rises, it drives the connecting tube 1201 to rise, so that each sampling needle 23 is aligned with the puncture hole at the bottom of the mixing disk 16. During this process, the sampling needle 23 contacts the rubber seal to give downward pressure to the magnetic suction cup 19, thereby separating the groove of the inner circle of the magnetic suction cup 19 and the insert of the connecting tube 1201, so that the magnetic suction cup 19 can also rotate During the rising process of the second cylinder 12, the sampling needle 23 is inserted into the rubber cover of the sampling tube, and then the head of the sampling needle 23 enters the interior of the sampling tube, and then the motor 3 is driven to rotate at a low speed. When the motor 3 rotates, the belt 4 is rotated, and the rotating rod 15 is rotated during the rotation of the belt 4. The rotation of the rotating rod 15 drives the driving gear to rotate. The driving gear and each first gear 8 are in a meshing state. During its rotation, the first gear 8 can drive each transmission rod 13 to rotate, so that the gasket 14 drives the sampling tube to rotate. The rubber cover of the sampling tube and the top of the mixing disk 16 are in sliding contact. Therefore, during the rotation of the motor 3, the liquid to be tested in the sampling tube is centrifugally mixed, so that the concentration of the liquid to be tested is increased. During the mixing process of the sampling tube, the liquid to be tested in the sampling tube overflows into the sampling needle 23, so that the liquid to be tested in the sampling tube is drained into the discharge cavity 2201 inside the sampling needle 23 and the magnetic head seat 22 for storage.The control ball 24 in the discharge chamber 2201 acts on the bottom of the discharge chamber 2201 due to the force of gravity, so that the bottom of the discharge chamber 2201 is in a blocked state. Then, after each magnetic head seat 22 takes a sample, the second cylinder 12 can be driven to descend and reset again, so that the sampling needle 23 and the sampling tube are separated. The rubber cover of the sampling tube has elasticity to seal the bottom of the sampling tube to prevent the test liquid inside the sampling tube from flowing out. Then, the second gear 11 is driven to rotate again (a second motor can be installed on the top of one of the second gears 11, not shown). When one of the second gears 11 rotates, , can drive the chain belt 9 to rotate. When the chain belt 9 rotates, the magnetic seat 30 can contact the side wall of the magnetic head seat 22. At the same time, the chipper 31 is located in front of the magnetic seat 30, and each magnetic head seat 22 on the magnetic suction disk 19 is shoveled onto each magnetic seat 30. In this process, there is a gap in the middle of the two chippers 31, which can be used to place the material control ball 24, so that when the magnetic head seat 22 is transferred to the magnetic seat 30, the sampling needle 23 and the magnetic head seat 22 do not overflow. Since the internal volume of each discharge cavity 2201 and the sampling needle 23 connected to the magnetic head seat 22 is equal, quantitative sampling can be performed;, A base 10 is fixedly connected to one side of the top of the placement table 6, a second cylinder 12 is fixedly connected to the middle of the base 10, and a quantitative sampling device for extracting the liquid to be tested in the sampling tube is installed on the top of the second cylinder 12; the quantitative sampling device includes a connecting tube 1201 fixed to the top of the second cylinder 12, a magnetic suction cup 19 is installed on the top of the connecting tube 1201, and a plurality of magnetic head seats 22 are connected to the top of the magnetic suction cup 19 by magnetic attraction, and a sampling needle 23 is fixedly connected to the bottom of each magnetic head seat 22, and each sampling needle 23 corresponds to the bottom of the puncture hole; the quantitative sampling device also includes an insert arranged on the top of the outer ring of the connecting tube 1201, and a groove corresponding to the insert is provided on the inner ring of the magnetic suction cup 19, a second spring 20 is fixedly connected to the inside of the connecting tube 1201, and a lifting plate 21 is fixedly connected to the top of the second spring 20, The lifting plate 21 extends toward the bottom of the magnetic suction cup 19 and contacts the bottom of the magnetic suction cup 19; each magnetic head seat 22 is provided with a discharge cavity 2201, and a control ball 24 is movably connected inside the discharge cavity 2201. When the chip 31 contacts the bottom of the magnetic head seat 22, the magnetic head seat 22 and the magnetic suction seat 30 are attracted together by magnetism. When the top piece 29 contacts the control ball 24, the control ball 24 is located inside the discharge cavity 2201 and moves; the control ball 24 is made of metal, and its surface is polished; each discharge cavity 2201 is connected to the sampling needle 23, and has an inner top side wall extending downward to form a shape with a wide upper part and a narrow lower part. The bottom of the discharge cavity 2201 is consistent with the control ball 24, and one end of the docking station 7 is detachably connected to a collecting bin 25, and the top of the collecting bin 25 is connected to the bottom of the spray chamber 26.

[0020] When one of the magnetic seats 30 is displaced to the top of the top piece 29, the chip 31 passes over the side wall of the top piece 29, and the material control ball 24 contacts the top piece 29, so that the material control ball 24 rolls on the top of the top piece 29. At the same time, the top piece 29 allows the material control ball 24 to push up, opening the discharge chamber 2201 inside the magnetic head seat 22, so that the test liquid drips from the inside of the discharge chamber 2201 along the top piece 29 into the reagent box 27, wherein the developing area inside the reagent box 27 reacts with the test liquid, and then drives the first cylinder 5 to descend. When the first cylinder 5 descends, the detector 501 is close to the top of the reagent box 27 to analyze and detect the nucleic acid. After recording the current data, the top piece 29 and the reagent box 27 are manually taken out for replacement, and when the chain belt 9 drives the sampling needle 23 and the magnetic head seat 22 to be located inside the spray chamber 26, the spray chamber 26 also contains the top piece 29, wherein the drainage machine 2 can be repeatedly filled with disinfectant, and then the pump on the drainage machine 2 is turned on to spray the disinfectant into the outside and inside of the sampling needle 23 and the magnetic head seat 22. When the material control ball 24 contacts the top plate 29 again, the disinfectant enters the sampling needle 23 and the discharge cavity 2201 to thoroughly disinfect them. The disinfected disinfectant flows into the collection bin 25 and is poured out manually after it is full. The reagent kit 27 and the top plate 29 are detachable and disposable. The device can disinfect the sampling needle 23 and the magnetic head seat 22 at the same time during the detection of the results. The two operations are synchronized, which increases the number of samples detected by the device in a short time and reduces the detection time of the samples. After each sampling needle 23 and the magnetic head seat 22 are disinfected, all the sampling tubes above the mixing disk 16 are manually taken out for replacement, and the magnetic head seat 22 is put back on the magnetic suction cup 19, making the device simple to operate.

[0021] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0022] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A nucleic acid detection and analysis device with a mixing structure, comprising a detection box (1), characterized in that: A placing table (6) is fixedly installed at the bottom of the detection box (1), and the placing table (6) is rotatably connected to driving rods on all sides, and a pair of second gears (11) are fixed on each of the driving rods, and a chain belt (9) is sleeved on the plurality of second gears (11). A plurality of magnetic seats (30) are fixedly installed on the inner ring of the chain belt (9), and a pair of chippers (31) are fixedly connected to one side of each of the magnetic seats (30). A docking table (7) is fixedly installed on the top of the placing table (6), and both sides of the top of the docking table (7) are detachably connected to insertion rods (28). Each The top of each of the insertion rods (28) is fixedly connected to a top plate (29); a drainage machine (2) is fixedly connected to the middle of one side of the outside of the detection box (1); a spray pressure chamber (26) is fixedly arranged on one side of the inside of the detection box (1); the spray pressure chamber (26) and the drainage machine (2) are fixedly connected; one of the top plates (29) is located inside the spray pressure chamber (26); a reagent box (27) is detachably connected to one side of the docking station (7); a first cylinder (5) is suspended at the top of the inside of the detection box (1); and a detector (501) is fixedly connected to the bottom of the first cylinder (5); A mixing structure for mixing the sampling tube is fixedly connected to one side of the placement table (6). After the mixing structure contacts the chip (31), the liquid to be tested is transferred to the inside of the reagent box (27) for testing via the chain belt (9).

2. A nucleic acid detection and analysis device with a mixing structure according to claim 1, characterized in that: The mixing structure comprises a plurality of transmission rods (13) rotatably connected to the top of the detection box (1), each transmission rod (13) being fixedly connected to a gasket (14) at the bottom, each transmission rod (13) being fixedly connected to a first gear (8) at the top, a motor (3) being fixedly connected to the top of the detection box (1), a belt (4) being sleeved on an output shaft of the motor (3), a rotating rod (15) being sleeved on one end of the belt (4), one end of the rotating rod (15) being suspended inside the detection box (1) by passing through the detection box (1), a center block (18) being slidably connected to the bottom of the rotating rod (15), a mixing disk (16) being slidably connected to the outside of the center block (18), a first spring (17) being fixedly connected to the bottom of the rotating rod (15), the first spring (17) being fixedly connected to the center block (18), a driving gear being fixedly mounted on the top of the rotating rod (15), the driving gear being meshed with each of the first gears (8).

3. A nucleic acid detection and analysis device with a mixing structure according to claim 2, characterized in that: The middle of the mixing plate (16) is recessed downward to form a placement area, the placement area is annular in shape, and a plurality of puncture holes are provided at the bottom, and the placement area is slidably connected to the sampling tube.

4. A nucleic acid detection and analysis device with a mixing structure according to claim 1, characterized in that: A base (10) is fixedly connected to one side of the top of the placement table (6), a second cylinder (12) is fixedly connected to the middle of the base (10), and a quantitative sampling device for extracting the liquid to be tested inside the sampling tube is installed on the top of the second cylinder (12).

5. A nucleic acid detection and analysis device with a mixing structure according to claim 4, characterized in that: The quantitative sampling device comprises a connecting tube (1201) fixed to the top of the second cylinder (12), a magnetic chuck (19) being installed on the top of the connecting tube (1201), the top of each of the magnetic chucks (19) being connected to a plurality of magnetic head seats (22) by magnetic attraction, the bottom of each of the magnetic head seats (22) being fixedly connected to a sampling needle (23), and each of the sampling needles (23) corresponding to the bottom of the puncture hole.

6. A nucleic acid detection and analysis device with a mixing structure according to claim 5, characterized in that: The quantitative sampling device further comprises an insert sheet arranged at the top of the outer ring of the connecting tube (1201); the inner ring of the magnetic suction cup (19) is provided with a groove corresponding to the insert sheet; a second spring (20) is fixedly connected inside the connecting tube (1201); a lifting plate (21) is fixedly connected to the top of the second spring (20); the lifting plate (21) extends toward the bottom of the magnetic suction cup (19) and contacts the bottom of the magnetic suction cup (19).

7. A nucleic acid detection and analysis device with a mixing structure according to claim 5, characterized in that: Each of the magnetic head seats (22) is provided with a material discharge cavity (2201) inside, and a material control ball (24) is movably connected inside the material discharge cavity (2201). When the chip (31) contacts the bottom of the magnetic head seat (22), the magnetic head seat (22) and the magnetic seat (30) are attracted together by magnetism, and when the top plate (29) contacts the material control ball (24), the material control ball (24) is located inside the material discharge cavity (2201) and moves.

8. A nucleic acid detection and analysis device with a mixing structure according to claim 7, characterized in that: Each of the discharge cavities (2201) is in communication with the sampling needle (23), and has an inner top side wall extending downward to form a shape that is wide at the top and narrow at the bottom. The bottom of the discharge cavity (2201) is aligned with the control ball (24).

9. A nucleic acid detection and analysis device with a mixing structure according to claim 7, characterized in that: The material control ball (24) is made of metal, and its surface is polished.

10. A nucleic acid detection and analysis device with a mixing structure according to claim 1, characterized in that: One end of the docking platform (7) is detachably connected to a collecting bin (25), and the top of the collecting bin (25) is in communication with the bottom of the spray chamber (26).

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