Method for grafting large-fruit elaeagnus angustifolia on middle-age forest elaeagnus angustifolia stock

Abstract

The invention discloses a method for grafting large-fruit elaeagnus angustifolia on a middle-age forest elaeagnus angustifolia rootstock, and belongs to the technical field of plant grafting, the method comprises the following steps: (1) cutting a single side of the lower end of a large-fruit elaeagnus angustifolia scion to a sickle shape to a lignified part, and peeling the other side of the scion to obtain a cutting; the length of the cutting slips is 15-20 cm, the lower ends of the cutting slips are sickle-shaped, and 2-3 plump buds are reserved at the upper ends of the cutting slips; (2) cutting the elaeagnus angustifolia stock, covering the cross section with a preservative film after water drops from the cross section, and keeping the cross section wet; (3) opening a hole in the preservative film on the section of the stock in the step (2), and inserting the lower end of the cutting slip in the step (1) for 10cm along the cambium of the stock; wherein the diameter at breast height of the stock is 10-30 cm, and the number of the cuttings is 2-5; (4) bagging the grafting cutting slips and the grafting positions of the grafting cutting slips and the rootstocks, and binding, knotting and fastening with ropes to prevent excessive evaporation; (5) when the new branches of the scions grow to 5-8 cm, the bagging is removed; and (6) management after grafting. Most of existing elaeagnus angustifolia forests are large-area middle-age forests or nearly-matured forests and are large in diameter at breast height, however, the grafting method limited by the invention is suitable for modification and optimization of the existing elaeagnus angustifolia forests and has a long grafting window, meanwhile, 2-5 grafting cutting slips are arranged on the cross section of a stock, the grafting survival rate is greatly increased, the large-fruit elaeagnus angustifolia fine variety is modified and optimized through elaeagnus angustifolia, and the yield of the large-fruit elaeagnus angustifolia is increased. The method has the advantages that large economic benefits can be generated, popularization of excellent varieties of the large-fruit elaeagnus angustifolia is promoted, enthusiasm of farmers to plant and manage and protect the protection forest is improved, and the method is of great significance in promoting sustainable development of the regional protection forest.

Description

Technical Field

[0001] The invention belongs to the technical field of plant grafting, and in particular relates to a method for grafting large-fruited Elaeagnus angustifolia onto a middle-aged Elaeagnus angustifolia rootstock. Background Art

[0002] Elaeagnus angustifolia is one of the main tree species in the Xinjiang Uygur Autonomous Region's shelterbelt construction. The planting areas are mostly in areas with relatively poor ecological environment, such as the edge of the desert, medium-to-severe saline-alkali land, and the periphery of rural residential areas. Over the years, most of the early Elaeagnus angustifolia forests have grown into middle-aged forests or nearly mature forests. Although they have played a good ecological role in farmland protection, soil and water conservation, and saline-alkali soil improvement, their economic benefits lag behind, and sustainable development is difficult to maintain.

[0003] Big-fruited Elaeagnus angustifolia is a large-fruited variety of Elaeagnus angustifolia. It is called Big Elaeagnus angustifolia or Big-fruited Elaeagnus angustifolia in southern Xinjiang Uygur Autonomous Region. It is a deciduous small tree or tree of the genus Elaeagnus in the family Elaeagnaceae. It is an ecological and economic dual-purpose tree species with a tree height of up to 10m. Its rich and fragrant aroma is called "fragrant osmanthus in the desert". It also has the characteristics of fast growth, resistance to wind and sand, tolerance to barrenness, and tolerance to salt and alkali. It is the main tree species for windbreak and sand fixation, improvement of saline-alkali land (sand land), and local greening in the northwest region. Elaeagnus angustifolia branches, leaves, flowers and fruits are all worth developing and utilizing, with high economic, ecological, medicinal and ornamental value, and broad prospects for development and utilization value. At present, it is mainly used in food, medicine, cosmetics, papermaking, forage, etc. In addition, since the large-fruited Elaeagnus angustifolia contains a variety of nutrients, its processing and utilization potential can be fully explored; therefore, the large-scale planting of the large-fruited Elaeagnus angustifolia not only plays an important role in preventing wind and sand and maintaining the ecological security of the oasis, but also, as a path for the development and utilization of food and energy and industrialization, it can generate greater economic benefits.

[0004] Gulinisa Kasimu, Abdurexit Reheman, Gao Mingyue, et al. Grafting and seedling raising technology of Elaeagnus angustifolia in Xinjiang Uygur Autonomous Region [J]. Shelterbelt Science and Technology, 2011, (04): 107-114. The research shows that the main grafting methods of Elaeagnus angustifolia are bark grafting and tongue grafting, and the grafting survival rate can reach more than 90%, but the young Elaeagnus angustifolia forest with a ground diameter of 2-5 cm is selected as the rootstock.

[0005] Bark grafting: Cut the bark longitudinally at the grafting site, deep into the wood, so that the scion can be inserted. The scion used has 2-4 buds, and the lower end on the same side of the uppermost bud is cut into a 3-5cm long horse ear-shaped slope. The entry point should be steeper, and then it is cut straight forward into a horse ear shape. The lower end of the opposite side of this cut surface is cut into a 36mm small cut surface, and then the long cut surface of the scion is inserted inward into the cut of the rootstock, aligned with the cambium, and then the interface (and the cross section of the rootstock) is tied and sealed with plastic film. If the rootstock is thick, 2-3 scions can be inserted to facilitate healing.

[0006] Tongue grafting: When grafting with tongue grafting, first cut the rootstock 30-50cm above the ground, remove all branches under the cut, and then select a scion with a branch diameter similar to that of the rootstock. The cut of the rootstock and the lower end of the scion are cut into 3-4cm straight and equal length bevels. At 1 / 3 of the near light end of the bevel, cut a longitudinal cut along the wood grain, and the length exceeds 1 / 3 of the bevel cut. Insert the beveled surface and longitudinal cut of the scion and the rootstock relative to each other, align one branch surface of the interface, and wrap it tightly with a film tape to keep it moist and prevent it from drying out.

[0007] Over the years, the large-scale Elaeagnus angustifolia planted in the early stage has grown into middle-aged forests or nearly mature forests, but the above two grafting methods are not suitable for the existing middle-aged forests or nearly mature forests of Elaeagnus angustifolia, and are only suitable for young trees with a rootstock diameter of 2-5 cm. Therefore, it is impossible to make better use of the existing Elaeagnus angustifolia shelterbelt to give full play to its greater economic and ecological benefits.

[0008] Therefore, there is an urgent need to provide a method for grafting large-fruited Elaeagnus angustifolia on middle-aged Elaeagnus angustifolia rootstocks, which can not only solve the problem of improving Elaeagnus angustifolia varieties, but also generate greater economic benefits. Summary of the invention

[0009] In view of the above technical problems, the present invention proposes a method for grafting large-fruited Elaeagnus angustifolia on middle-aged Elaeagnus angustifolia rootstocks.

[0010] To achieve the above object, the present invention provides the following technical solutions:

[0011] The technical solution of the present invention is:

[0012] A method for grafting large-fruited Elaeagnus angustifolia on a middle-aged Elaeagnus angustifolia rootstock, comprising the following steps:

[0013] (1) Cutting the lower end of the large-fruited Elaeagnus angustifolia spike into a sickle shape on one side to the lignified part, and peeling the epidermis on the other side to obtain a cutting; wherein the cutting is 15-20 cm long, the lower end is sickle-shaped, and 2-3 full buds are retained on the upper end;

[0014] (2) Cut the rootstock of Elaeagnus angustifolia dry, drip a small amount of water on the cross section, and cover it with plastic wrap to keep the cross section moist;

[0015] (3) making a hole in the cling film on the cross section of the stock to facilitate the insertion of the lower end of the cutting into the stock; specifically: inserting the lower end of the cutting into the cambium layer of the stock by 10 cm;

[0016] Among them, the rootstock diameter at breast height is 10-30cm, and the number of cuttings is 2-5;

[0017] (4) Put the grafted cuttings and the joints with the stock into a fresh-keeping bag and tie them with cotton rope or nylon rope for about 1 year until the stock and the grafting joint are completely healed, and the grafting is completed;

[0018] Among them, putting it in a fresh-keeping bag can prevent excessive evaporation, maintain a certain humidity and temperature, and promote the germination of cuttings;

[0019] (5) When the new branches of the scion grow to 5-8 cm, remove the bag;

[0020] (6) Management after grafting.

[0021] Preferably, the large-fruited Elaeagnus angustifolia spikelets in step (1) need to be collected before germination, and the spikelets are annual, lignified, and have full buds.

[0022] Beneficial effects: The cuttings are collected before germination and are in a dormant state. After grafting, the combined part of the rootstock and the cuttings is the cambium, which can continuously divide to form callus tissue and heal with each other.

[0023] Furthermore, the spikelets are annual woody branches with full buds from an excellent variety of large-fruited Elaeagnus angustifolia.

[0024] Preferably, the large-fruited Elaeagnus angustifolia spikes in step (1) need to be stored at low temperature before cutting.

[0025] Beneficial effects: After collecting the large-fruited Elaeagnus angustifolia cuttings, storing them at low temperatures can put the cuttings into a dormant state, reduce water evaporation and nutrient consumption, and promote the survival rate of the cuttings.

[0026] Preferably, the low temperature storage conditions are: temperature of 0°C--5°C, humidity of 80%-90%, and low temperature storage until the spikes are used.

[0027] Preferably, the low temperature storage includes a temperature-controlled storage cellar or a temporary storage cellar;

[0028] The temperature-controlled storage cellar is as follows: the temperature of the large-fruited Elaeagnus angustifolia spikelets is adjusted to -1°C--5°C, the humidity is preferably 80%-90%, and appropriate gaps are left between the spikelets;

[0029] The temporary storage cellar is as follows: ice is placed in the storage cellar with a thickness of 30cm-50cm, the temperature of the storage cellar is maintained at 0°C, the collected large-fruited Elaeagnus angustifolia spikelets are bundled and placed upright on the ice layer with spaces left between the spikelets, and the cellar door is tightly covered.

[0030] Preferably, in step (2), the Elaeagnus angustifolia rootstock before trunk cutting needs to be irrigated 7-10 days before grafting.

[0031] Preferably, in step (3), the grafting time is from late February to early April in southern Xinjiang Uygur Autonomous Region or from mid-April to early May in northern Xinjiang Uygur Autonomous Region.

[0032] Beneficial effects: The grafting method of the present invention has a longer grafting window than the prior art, which greatly increases the flexibility of agronomic operations, allowing horticultural workers to choose the best time for grafting based on factors such as weather and labor arrangements; in addition, the longer grafting time window can better adapt to the uncertainty brought about by climate change and further improve the grafting survival rate.

[0033] Preferably, in step (3),

[0034] When the diameter at breast height of the Elaeagnus angustifolia rootstock after trunk cutting is 5-9cm, two cuttings are grafted on the surface of each rootstock cut;

[0035] When the diameter at breast height of the Elaeagnus angustifolia rootstock after trunk cutting is 10-12cm, three cuttings are grafted on the surface of each rootstock cut;

[0036] When the diameter at breast height of the Elaeagnus angustifolia rootstock after trunk cutting is 13-20cm, four cuttings are grafted on the surface of each rootstock cut;

[0037] When the breast diameter of the Elaeagnus angustifolia rootstock after trunk cutting is greater than 20 cm, 5 cuttings are grafted on the incision surface of each rootstock.

[0038] Preferably, the post-grafting management in step (6) specifically comprises the following steps:

[0039] Water thoroughly within 7 days after grafting;

[0040] 20 days after grafting, remove the buds;

[0041] When the new shoots grow to more than 20cm, they need to be tied with sticks;

[0042] Pruning.

[0043] Preferably, the specific operation of pruning is:

[0044] When the new branches of Elaeagnus angustifolia exceed 70-80cm, pinch off the top;

[0045] Starting from the second year, prune all the side branches of the rootstock every spring before the sap flows;

[0046] Carry out periodic pruning for 3-5 consecutive years.

[0047] The present invention solves the problem that the grafting of large-fruited Elaeagnus angustifolia is limited by the diameter at breast height of the Elaeagnus angustifolia rootstock, and compared with the prior art, the present invention specifically includes the following advantages and technical effects:

[0048] (1) The method of the present invention is not restricted by the diameter of the rootstock at breast height, and can be grafted not only on the rootstock of middle-aged and older forests, but also on the rootstock of young forests, and can also maintain the excellent characteristics of the large-fruited Elaeagnus angustifolia scion variety;

[0049] (2) The rootstock of the Elaeagnus angustifolia is a middle-aged or older forest, which has a well-developed root system and begins to bloom and bear fruit two years after the graft survives, effectively shortening the fruiting time;

[0050] (3) It can fully utilize the drought resistance and salt-alkali tolerance of the Elaeagnus angustifolia rootstock, and also give full play to the advantages of Elaeagnus angustifolia, such as large fruit, good taste, and high economic benefits;

[0051] (4) The survival rate of the grafted scion of Elaeagnus angustifolia is high, reaching more than 95%, which achieves the purpose of efficient reproduction and can create higher economic benefits.

[0052] Therefore, the grafting method provided by the present invention can not only promote the large-scale production and industrial development of large-fruited Elaeagnus angustifolia and meet the market demand for large-fruited Elaeagnus angustifolia fruits, but also meet the needs of domestic and neighboring countries as the scale continues to expand. BRIEF DESCRIPTION OF THE DRAWINGS

[0053] The drawings constituting a part of the present application are used to provide a further understanding of the present application. The illustrative embodiments and descriptions of the present application are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0054] Figure 1 This is a photo of the invention grafting large-fruited Elaeagnus angustifolia on the middle-aged Elaeagnus angustifolia rootstock;

[0055] Figure 2 The present invention is a schematic diagram of the process of grafting large-fruited Elaeagnus angustifolia onto middle-aged Elaeagnus angustifolia rootstocks. DETAILED DESCRIPTION

[0056] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0057] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0058] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0059] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0060] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0061] The present invention provides a method for grafting large-fruited Elaeagnus angustifolia on a middle-aged Elaeagnus angustifolia rootstock, comprising the following steps:

[0062] 1. Scion Collection

[0063] After the leaves fall in autumn and before the buds sprout in spring, collect the scions from the middle and upper part of the crown of the excellent large-fruited Elaeagnus angustifolia mother trees. Each tree can collect 30-50 scions, and every 100 scions are bundled for spare use.

[0064] 2. Storage of scion

[0065] 21. Temperature-controlled storage cellar

[0066] After the scion is harvested, the scion should be properly stored; it can be placed in a temperature-controlled storage cellar with the temperature adjusted to -1℃--5℃ and the humidity preferably 80%-90%, with appropriate gaps between the scions;

[0067] 22. Temporary storage cellar

[0068] If there is no temperature-controlled storage cellar, a temporary storage cellar can be used; the storage cellar is 2.0m-2.5m wide and 1.5m-1.8m deep, and the length is determined by the number of spikelets; ice is placed in the storage cellar before the spikelets are collected, with a thickness of 30cm-50cm, and the temperature of the storage cellar is kept at around 0℃. The collected spikelets are bundled and placed upright on the ice layer, leaving gaps between the spikelets, and the cellar door is tightly covered;

[0069] 3. Grafting time

[0070] Late February to early April in southern Xinjiang Uyghur Autonomous Region or mid-April to early May in northern Xinjiang Uyghur Autonomous Region;

[0071] 4. Cut the cuttings

[0072] Select cuttings with full buds, strong branches, no sprouting, no dehydration, no decay, and no diseases and insect pests, cut cuttings with a length of 15cm-20cm, retaining 2-4 buds, and immediately soak them in a small bucket filled with clean water;

[0073] 5. Grafting method

[0074] Cut the cuttings 8cm-10cm apart on one side into a sickle shape to the lignified part, and peel off the cuttings epidermis on the other side;

[0075] Cut the Elaeagnus angustifolia rootstock 40-80cm from the ground, drip water on the cross section to keep it moist, and then cover it with a layer of plastic wrap to prevent the cut section from being drained of water from the rootstock due to excessive transpiration; then make holes in the plastic wrap, insert the cut scion incision completely into the phloem of the rootstock, and according to the diameter at breast height of the rootstock, 3-4 scions can be grafted, and then put on a white plastic bag, use a plastic rope to spirally tie upwards at the lower part of the interface, and then wrap it from the upper part to the middle and lower part, tie a knot and tighten it; the white plastic bag should completely wrap the scion and the cross section of the rootstock; when the new branches of the scion grow to 5-8cm, remove the bag to expose the new branches to the air to prevent burning the seedlings;

[0076] 6. Management after grafting

[0077] Irrigation: Fully irrigate within 7 days after grafting to ensure survival rate;

[0078] Bud removal: 20 days after grafting, bud removal should be done;

[0079] Tie the sticks: When the new shoots grow to more than 20cm, tie the sticks to prevent them from being broken by the wind and affecting the normal growth of effective branches. The sticks should be about 20cm longer than the cut of the rootstock. Tie a stick to each joint to fix the new shoots on the sticks;

[0080] Pruning: When the new branches of Elaeagnus angustifolia exceed 70-80cm in the current year, it is advisable to pinch the top to promote branch growth; starting from the second year after the grafting, prune once every spring before the sap flows, and cut off all the side branches of the rootstock that affect the growth of the scion; cut off all the side branches of the rootstock for 3-5 consecutive years to ensure that the scion can form a new crown;

[0081] In some preferred embodiments, the cambium of the rootstock and scion must be aligned.

[0082] In some preferred embodiments, the joint of the stock and the scion is tightly tied with cotton rope or nylon rope to make the cambium close and maintain appropriate humidity.

[0083] In some preferred embodiments, the binding is released after the cuttings survive.

[0084] In some preferred embodiments, the length of the cuttings depends on the strength of the interface. A long cutting is used for a strong one and a short cutting is used for a weak one, but at least three buds are left.

[0085] Existing grafting methods, namely, the bark-insertion method and the tongue-in-gluing method, are not suitable for grafting of middle-aged Elaeagnus angustifolia as rootstocks. The tongue-in-gluing method requires that the cuttings be close in thickness to the rootstock thickness; the bark-insertion method requires that the bark be cut longitudinally at the grafting site of Elaeagnus angustifolia, reaching the xylem, but the diameter at breast height of the middle-aged Elaeagnus angustifolia is generally 10-30cm, and the bark is thicker, so it is impossible to cut longitudinally. Elaeagnus angustifolia belongs to hard-tied wood, and its texture is hard, and the wound healing at the grafting site is slow. If cuttings with a horse-ear-shaped inclined surface of 3-5cm long are also used, it is easy to be broken by the wind or the grafting site will break before healing due to the fast growth of the large-fruited Elaeagnus angustifolia. The grafting method of the present invention overcomes the limitation of the existing large-fruited Elaeagnus angustifolia in selecting the rootstock diameter at breast height, and can not only be grafted on the rootstocks of middle-aged and above forest ages, but also can be used for the young forest rootstocks. That is, the method provided by the present invention is more suitable for the current situation of grafting and breeding of large-fruited Elaeagnus angustifolia and improving varieties.

[0086] The above-mentioned grafting method defined by the present invention achieves the following technical effects:

[0087] (1) Social benefits

[0088] The application of the grafting method of the present invention can effectively radiate and drive the planting of excellent varieties of large-fruited Elaeagnus angustifolia in the surrounding areas, improve quality and increase efficiency; it can also provide farmers with a full range of services such as new and excellent varieties of large-fruited Elaeagnus angustifolia, scientific planting techniques, and efficient seedling breeding techniques, which plays a positive role in promoting the development of the industrialization of large-fruited Elaeagnus angustifolia.

[0089] (2) Economic Benefits

[0090] The application of the grafting method of the present invention can improve the existing varieties of Elaeagnus angustifolia, so that the Elaeagnus angustifolia with only ecological benefits can be transformed into large-fruit Elaeagnus angustifolia with both ecological and economic benefits, thus creating higher economic benefits. During the high-yield period, each large-fruit Elaeagnus angustifolia plant can produce an average of 50 kg of fruit. Based on the current purchase price of 10 yuan / kg, each large-fruit Elaeagnus angustifolia plant can generate a profit of 500 yuan. In addition, the branches, leaves, and flowers of the large-fruit Elaeagnus angustifolia also have development and utilization value, which can promote the improvement of forestry ecology quality and efficiency.

[0091] The technical solution of the present invention is further illustrated by the following embodiments.

[0092] Example 1

[0093] A method for grafting large-fruited Elaeagnus angustifolia on a middle-aged Elaeagnus angustifolia rootstock, comprising the following steps:

[0094] 1. Scion Collection

[0095] After the leaves fall in autumn and before the buds sprout in spring, collect the branches from the middle and upper part of the crown of excellent mother trees. Each tree can collect 30-50 branches, and each 100 branches form a bundle for future use.

[0096] 2. Storage of scion

[0097] After the scion is harvested, the scion should be properly stored in a temperature-controlled storage cellar with a temperature of (-1)-(-5)°C and a humidity of 80%-90%, leaving appropriate gaps between the scions.

[0098] 3. Grafting time

[0099] Southern Xinjiang Uyghur Autonomous Region at the end of February;

[0100] 4. Cut the cuttings

[0101] Select cuttings with full buds, strong branches, no sprouting, no dehydration, no decay, and no diseases and insect pests, cut cuttings with a length of 15cm-20cm, retaining 2-4 buds, and immediately soak them in a small bucket filled with clean water;

[0102] 5. Grafting method

[0103] Cut the cuttings 8cm-10cm apart on one side into a sickle shape to the lignified part, and peel off the cuttings epidermis on the other side;

[0104] 7-10 days before grafting, the Elaeagnus angustifolia rootstock should be irrigated, and then the Elaeagnus angustifolia rootstock should be cut at 40-80 cm from the ground, a small amount of water should be dripped on the cross section of the rootstock to keep it moist, and then covered with a layer of plastic wrap to prevent the cut section from being drained of water from the rootstock due to too fast transpiration; then a hole is made in the plastic wrap on the cross section of the rootstock, and the cut cutting cut is completely inserted into the phloem of the rootstock, and the rootstock with a breast diameter of 13-20 cm is selected, 4 scions are grafted, and then a white plastic bag is put on, and a plastic rope is used to spirally tie upwards at the lower part of the interface, and then it is wrapped from the upper part to the middle and lower part, and knotted and tied tightly; the white plastic bag should completely wrap the cutting and the cross section of the rootstock; when the new branches of the cuttings grow to 5-8 cm, the bag is removed to expose the new branches to the air to prevent burning the seedlings;

[0105] 6. Management after grafting

[0106] Irrigation: Fully irrigate within 7 days after grafting to ensure survival rate;

[0107] Bud removal: 20 days after grafting, bud removal should be done;

[0108] Tie the sticks: When the new shoots grow to more than 20cm, they need to be tied with sticks to prevent them from being broken by the wind and affecting the normal growth of effective branches. The length of the sticks should be about 20cm longer than the cut of the rootstock. Tie a stick to each joint to fix the new shoots on the sticks.

[0109] Pruning: When the new branches of the large-fruited Elaeagnus angustifolia exceed 70-80cm in the same year, it is advisable to pinch and prune to promote branch growth; starting from the second year after the grafting is successful, pruning should be carried out once every spring before the sap flows, and all side branches of the rootstock that affect the growth of the cuttings should be cut off; all side branches of the rootstock should be cut off for 3-5 consecutive years to ensure that the cuttings can form a new crown.

[0110] Example 2

[0111] The difference from Example 1 is that the rootstock diameter at breast height is 10-12 cm, and three cuttings are grafted.

[0112] Example 3

[0113] The difference from Example 1 is that the rootstock diameter at breast height is 5-9 cm, and two cuttings are grafted.

[0114] Example 4

[0115] The difference from Example 1 is that the diameter of the rootstock at breast height is 20-30 cm, and 5 cuttings are grafted.

[0116] Effect verification

[0117] A sampling survey was conducted on the grafted individual plants of Example 1 (C), Example 2 (B), Example 3 (A) and Example 4 (D), with 50 plants sampled in each case. The results of the grafted survival rate survey are shown in Table 1.

[0118] Table 1 Grafting survival rate survey form

[0119]

[0120] As can be seen from Table 1, according to the size of the rootstock DBH, 5-9cm, 10-12cm, 13-20cm and 20-30cm can be grafted with 2, 3, 4 and 5 cuttings respectively, and the cutting survival rates are 91%, 90.66%, 89.5% and 78.45% respectively, that is, A>B>C>D. The survival rate of grafted individual plants is as high as 98% for D, 96% for B and C, and the lowest for A at 94%, that is, D>C=B>A. The average survival rate of cuttings is 87.39%, and the average survival rate of grafted individual plants is 96.00%.

[0121] In addition, the younger the age of Elaeagnus angustifolia, the smaller the stock diameter at breast height, the larger the scion survival rate, and vice versa. Although D has the lowest survival rate of cuttings, each grafted individual plant cutting is 5, if there are 2 or more cuttings surviving on the grafted individual plant, 1-2 cuttings will also be retained according to the health, growth, and wound healing degree of the cuttings, and the remaining cuttings will all be cut off, and the purpose is to focus on cultivating 1-2 grafted individual plants, as long as the cuttings of the grafted individual plants at least guarantee survival of one, then the grafting survives. Therefore, the survival rate of D grafted individual plants is the highest, followed by B and C, and A is the lowest. So the grafting method provided by the present invention is not only suitable for low-age forest Elaeagnus angustifolia, but also more suitable for the grafting of Elaeagnus angustifolia forests of middle age and above age grades with a stock diameter at breast height of 10-30cm.

[0122] The above are only preferred specific implementations of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed in the present application should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

Claims

1. A method for grafting large-fruited Elaeagnus angustifolia on middle-aged Elaeagnus angustifolia rootstock, characterized in that: The following steps are involved: (1) Cutting the lower end of the large-fruited Elaeagnus angustifolia spike into a sickle shape on one side to the lignified part, and peeling the spike epidermis on the other side to obtain a cutting; the cutting is 15-20 cm long, the lower end is sickle-shaped, and 2-3 full buds are retained on the upper end; (2) Cut the Elaeagnus angustifolia rootstock dry, drip a small amount of water on the cross section of the rootstock, and cover it with plastic wrap to keep the cross section moist; (3) making a hole in the plastic wrap of the cross section of the stock in step (2), and inserting the lower end of the cutting in step (1) 10 cm along the cambium of the stock; Among them, the rootstock diameter at breast height is 10-30cm, and the number of cuttings is 2-5; (4) Bag the grafted cuttings and the joints with the rootstock, and tie them up with ropes to prevent excessive evaporation; (5) When the new branches of the scion grow to 5-8 cm, remove the bag; (6) Management after grafting.

2. A method for grafting large-fruited Elaeagnus angustifolia on middle-aged Elaeagnus angustifolia rootstock according to claim 1, characterized in that: The large-fruited Elaeagnus angustifolia spikelets described in step (1) need to be collected before germination. The spikelets are annual, lignified, and have full buds.

3. A method for grafting large-fruited Elaeagnus angustifolia on middle-aged Elaeagnus angustifolia rootstock according to claim 2, characterized in that: After the collection, the spikelets need to be stored at low temperature under the following conditions: temperature of 0°C-5°C and humidity of 80%-90% until the spikelets are used.

4. A method for grafting large-fruited Elaeagnus angustifolia on middle-aged Elaeagnus angustifolia rootstock according to claim 1, characterized in that: In step (2), the Elaeagnus angustifolia rootstock before trunk cutting needs to be watered 7-10 days before grafting.

5. A method for grafting large-fruited Elaeagnus angustifolia on middle-aged Elaeagnus angustifolia rootstock according to claim 4, characterized in that In step (3), when the diameter at breast height of the Elaeagnus angustifolia rootstock after stem cutting is greater than 20 cm, five cuttings are grafted; When the diameter at breast height of the Elaeagnus angustifolia rootstock is 13-20cm after trunk cutting, four scions are grafted; When the diameter at breast height of the Elaeagnus angustifolia rootstock after trunk cutting is 10-12cm, three scions are grafted; When the breast diameter of the Elaeagnus angustifolia rootstock is 5-9cm after trunk cutting, two scion grafts are used.

6. A method for grafting large-fruited Elaeagnus angustifolia on middle-aged Elaeagnus angustifolia rootstock according to claim 1, characterized in that: In step (3), the grafting time is from late February to early April in southern Xinjiang Uygur Autonomous Region or from mid-April to early May in northern Xinjiang Uygur Autonomous Region.

7. A method for grafting large-fruited Elaeagnus angustifolia on middle-aged Elaeagnus angustifolia rootstock according to claim 1, characterized in that: The post-grafting management in step (6) includes full watering within 7 days after grafting; removing buds 20 days after grafting; tying new shoots with sticks when they grow to more than 20 cm to protect the new shoots and the grafting surface; and pruning.

8. A method for grafting large-fruited Elaeagnus angustifolia on middle-aged Elaeagnus angustifolia rootstock according to claim 7, characterized in that: The specific operation of pruning is: when the new branches exceed 70-80cm, pinch the top to prevent the new branches from being too large and easily broken by the wind; untie the branches one year after grafting; starting from the second year, prune all the side branches of the rootstock before the sap flows in spring every year; and perform periodic pruning for 3-5 consecutive years.

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