Method for improving grafting survival rate of malania oleifera

Abstract

The invention relates to the technical field of plant grafting, in particular to a method for improving the grafting survival rate of malania oleifera, which comprises the following steps: S1, scion preparation: treating branches of excellent perennial wild malania oleifera plants in the field, conveying the branches to an inoculation field, and disinfecting to obtain disinfected branches; s2, stock preparation; s3, grafting: preparing a scion by using the disinfected branch, treating a stock, tightening the stock and a grafting film for tongue grafting of the scion, and smearing a healing agent on a cut of the cut stock and the upper end of the grafting film; s4, management after grafting. Compared with the prior art, the method has the advantages that superior plant branches and artificially cultivated stocks are subjected to tongue grafting, and compared with bud grafting, the yield time of grafted seedlings is shortened; and then the branch of the malania oleifera superior plant is treated immediately when the scion is collected, so that the problems that the activity of the branch is reduced in the transportation process and the formation of the callus of the stock and scion cambium is slow are solved, the grafting survival rate is increased, and the grafted seedling obtaining time is shortened.

Description

Technical Field

[0001] This invention relates to the field of plant grafting, specifically to a method for improving the survival rate of grafted garlic cloves. Background Technology

[0002] The garlic fruit (Malania oleifera Chunet SK Lee) is an evergreen tree belonging to the genus Malina in the family Aceraceae. It is also known as mountain tung fruit, Malanhou, Mengma, and monkey fruit. It is a rare and endangered national second-class protected plant unique to my country.

[0003] However, compared to artificially propagated seedlings, wild garlic cloves exhibit high heterozygosity due to dioecious and cross-pollination, leading to segregation of important economic traits such as nervonic acid content in offspring and decreased trait stability. Furthermore, the population of wild garlic cloves is declining annually due to environmental degradation and increasing tree age. To protect the germplasm resources of wild garlic cloves, grafting is employed to accelerate their propagation.

[0004] However, current research on grafting garlic cloves is limited to bud grafting. This method involves grafting onto tender hypocotyls, making it difficult and requiring advanced grafting techniques. Using 2-4 year old garlic clove trees as rootstock, both the rootstock and scion secrete resin, resulting in slow cambium bonding at the grafting site. This affects the grafting survival rate and the average scion germination time. Furthermore, since most superior garlic clove trees are found in the wild, the long-distance transportation of the grafted material causes resin leakage from the cut, water loss, and physiological damage, leading to decreased scion activity or even death. Consequently, the survival rate of tongue grafting is low. Summary of the Invention

[0005] The purpose of this invention is to provide a method for improving the grafting survival rate of garlic fruit, aiming to reduce the impact of long-distance transportation on the grafting survival rate, as well as the impact of the resin secreted by garlic fruit on the grafting survival rate.

[0006] To achieve the above objectives, the present invention provides a method for improving the grafting survival rate of garlic cloves, comprising:

[0007] S1, Scion preparation: Take branches from superior perennial wild garlic plants in the wild, immerse the branches in the soaking solution and cut them obliquely. After soaking for 20-60 minutes, cut them horizontally in the soaking solution. Wrap the horizontal cut with textile material moistened with the soaking solution and transport it to the inoculation site for disinfection. Obtain the disinfected branches. The components of the soaking solution include CaCl2, trehalose, azoxystrobin, chitosan oligosaccharide and salicylic acid.

[0008] S2, Rootstock Preparation: Select artificially cultivated garlic cloves as rootstock. Before grafting, continuously drench the roots with a pesticide, including salicylic acid, boric acid, etc. and sodium alginate;

[0009] S3, grafting method: prepare scions using the disinfected branches, treat the rootstock, tongue-graft the rootstock and scion and tie them tightly with grafting film, and apply healing agent to the cut of the polished rootstock and the upper end of the grafting film.

[0010] S4, post-grafting management.

[0011] Preferably, in the above technical solution, in step S1, the soaking solution is 0.5-0.8g. +0.2-1g trehalose + 5-13mg azoxystrobin + 0.1-0.3g chitosan oligosaccharide + 20-30mg salicylic acid + 100mL water.

[0012] According to the above technical solution, the principle of this invention in improving the activity of superior wild garlic plants is as follows: First, after obliquely cutting the branches, soak them in an infusion solution on-site for 20-60 minutes to create a larger absorption surface for the branches, allowing the infusion solution to be partially absorbed by the vascular system of the branches through the cut and transported to the tissues and cells of the branches; second, salicylic acid and chitosan oligosaccharides regulate stress response, maintain protein activity, reduce leaf transpiration and alleviate physiological damage caused by cutting, and also provide trehalose and exogenous... This process enhances the mechanical strength of cell walls and maintains the structural stability of cell membranes, reducing leakage of cell contents and decreasing resin secretion from branches, thus increasing the activity of branch cells and preventing a decline in grafting activity due to resin secretion and water loss during long-distance transport of branches. Furthermore, pyraclostrobin provides antiseptic and antibacterial protection, preventing disease infection through the cut. Finally, the flat cut and the wrapping of the cut with textile material soaked in the soaking solution continuously provide the branches with a liquid containing all the protective components.

[0013] Preferably, in the above technical solution, in step S2, the reagent is 20-30 mg salicylic acid + 60-80 mg boric acid + 0.5-0.8 g +1-3g trehalose + 0.1-0.3g chitosan oligosaccharide + 100mL water.

[0014] According to the above technical solution, the principle of using a drug to drench the roots before grafting to reduce rootstock sap flow is as follows: the roots absorb the drug, and the chitosan oligosaccharide and salicylic acid activate the rootstock's defense system, reduce root water absorption activity, weaken transpiration, and maintain the rootstock's water balance; calcium chloride provides exogenous calcium ions, trehalose and boric acid provide boron, reducing sap flow from the wound during pruning and grafting, while activating the healing mechanism, accelerating the healing at the graft union, and improving the grafting survival rate.

[0015] Preferably, in the above technical solution, in step S2, the rootstock is a robust 2-3 year old artificially cultivated garlic fruit.

[0016] Preferably, the specific steps of the grafting method described in step S3 are as follows:

[0017] S31, Prepare scions using the disinfected branches: Resurrect the disinfected branches, cut the resurrected branches into branches that are 4-8cm long, cut them obliquely into a horse ear-shaped cut, and make a 1-2cm deep vertical cut at the lower 1 / 3 of the horse ear-shaped cut to form a tongue-shaped structure to obtain scions.

[0018] S32, cut the rootstock short at a height of 15-30cm, make a slanted cut in the shape of a horse ear at a distance of 8-10cm from the cross-section of the rootstock, and make a vertical cut 1-2cm deep at the upper 1 / 3 of the horse ear-shaped cut to form a tongue-shaped structure.

[0019] S33, after aligning the scion with the rootstock cut, bind the branch and rootstock tightly with grafting film, and apply healing agent to the cut of the polished rootstock and the upper end of the grafting film.

[0020] Preferably, in the above technical solution, the healing agent is 1-3g sodium alginate + 50-70mg NAA + 7-10g activated charcoal + 0.5-1g PVP + 100mL water.

[0021] According to the above technical solution, the present invention applies a healing agent to the cut end of the rootstock, which physically isolates the wound from the external environment, reduces the rootstock from pathogen infection, releases NAA, induces dedifferentiation of parenchyma cells, and promotes wound healing of the rootstock.

[0022] Preferably, in the above technical solution, the step of reviving the disinfected branches involves cutting off the cut ends of the disinfected branches and then inserting the cut branches into an activator for 3-4 hours to allow the branches to fully absorb the activator.

[0023] Preferably, in the above technical solution, the activator is 10-20mg glycine + 10-20mg glutamic acid + 20-30mg betaine + 5-10mg sterol + 60-70mg trehalose + 5-15mg glutathione + 50-70mg NAA + 100mL water.

[0024] According to the above technical solution, the principle that soaking the scion with the activator can promote efficient healing between the rootstock and the scion is as follows: Glutathione removes reactive oxygen species from the scion wound, reducing oxidative damage to the scion, and, combined with betaine and trehalose, protects the integrity of the membrane structure, jointly maintaining the activity of scion cells and proteins; NAA, as an auxin signal, stimulates cambium cells to divide and enter the division cycle, and, combined with glutamate, glycine, and sterols, provides the key components required for cambium cell division, accelerating the formation of new cambium cells at the grafting site, shortening the grafting survival time, and improving the grafting survival rate.

[0025] Preferably, in the above technical solution, the disinfection involves immersing the branches in 600 times diluted carbendazim solution for 5-10 minutes.

[0026] Preferably, in the above technical solution, the post-grafting treatment specifically includes: controlling the cultivation temperature to 27-30℃ and the cultivation humidity to 75-85%; promptly removing newly sprouted buds from the rootstock; promptly regrafting rootstocks that show no signs of sprouting after 30 days; and applying water and fertilizer 1-2 times per month when the scion length is ≥10cm.

[0027] Preferably, in the above technical solution, the water fertilizer is made of 15-20 mg / L potassium sulfate, 15-20 mg / L potassium dihydrogen phosphate, 4-6 mg / L magnesium sulfate, 6-10 mg / L ferrous sulfate, 3-5 mg / L zinc sulfate, and 1-5 mg / L sodium alginate.

[0028] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0029] (1) After scion collection and during transportation, the cut surfaces are wrapped with a soaking solution to reduce the problem of resin leakage, water loss, and physiological damage to the scion branches, which leads to a decrease in the activity of the branches of superior wild plants. The principle of the soaking solution treatment to avoid the decrease in activity of the branches during transportation is as follows: salicylic acid, chitosan oligosaccharide, trehalose and exogenous It enhances the mechanical strength of the cell wall and maintains the structural stability of the cell membrane, reduces leaf transpiration, reduces the leakage of cell contents, and prevents the flow of resin and water from the cut, thus avoiding a decrease in grafting activity. The soaking solution promotes the balance between cell membrane lipids and the inside and outside of the protoplast layer, enhances the water retention capacity of the branches, and adds the antibacterial component pyraclostrobin to the soaking solution to prevent microbial infection from the cut, which could lead to grafting failure.

[0030] (2) Applying a chemical to the rootstock before grafting reduces sap flow from the pruning and grafting wounds, while activating the healing mechanism, accelerating the healing at the interface, improving the cambium binding capacity of the rootstock grafting site, and shortening the average grafting survival time of garlic fruit.

[0031] (3) Improve the survival rate of tongue grafting. Compared with bud grafting, tongue grafting can shorten the seedling growth time of grafted seedlings. Attached Figure Description

[0032] Figure 1 This is a bar chart showing the water loss rate of branches in different soaking solutions in Example 1 of this invention;

[0033] Figure 2 This is a linear graph showing the viability of branches under different soaking solutions in Example 1 of this invention.

[0034] Figure 3 This is a diagram illustrating the successful grafting of garlic cloves in a specific embodiment of this invention.

[0035] Figure 4 This is a diagram illustrating the successful grafting of garlic cloves in a specific embodiment of this invention.

[0036] Figure 5 This is a diagram of the successful grafting of garlic cloves in a specific embodiment of the present invention. Detailed Implementation

[0037] The technical solutions in the embodiments of the present invention have been clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention. Example 1

[0038] A method for improving the grafting survival rate of garlic cloves, the method comprising:

[0039] Using 1-2 year old garlic clove branches from the cultivation base as the experimental subjects, the branches were cut at an angle and then soaked in equal volumes of distilled water, soaking solution 1, soaking solution 2, soaking solution 3, soaking solution 4, soaking solution 5, and soaking solution 6 for 25 minutes. After removing and drying the surface liquid, each branch was labeled, and its weight (w1) was measured. The cell dehydrogenase activity (OD1) was detected using TTC staining. The branches were then placed at 35℃ for 8 hours, and the weight (w2) and cell dehydrogenase activity were measured again. Cellular dehydrogenase activity of 1-2 year old garlic fruit branches boiled at 100℃ for 10 minutes , The method for detecting cellular dehydrogenase activity is as follows: Water loss rate and vitality value, such as Figure 1-2 As shown. The above experiment was conducted in 3 replicates, with 3 healthy branches in each replicate.

[0040] Table 1 Components of the soaking solution

[0041]

[0042] from Figure 1 and Figure 2 It can be seen that the 1-2 year old garlic shoots treated with soaking solution 3 had the lowest water loss rate and better activity. This may be because the components of soaking solution 3 enhance the mechanical strength of the cell wall and maintain the structural stability of the cell membrane, maintain the balance of cell membrane lipids and the inside and outside of the protoplast layer, resulting in higher water retention capacity and slower water loss from the shoots. At the same time, its components promote the maintenance of cell dehydrogenase activity, thereby improving the vitality of the shoots.

[0043] Scion preparation: When collecting scions in the field, carry an appropriate amount of soaking solution, a sealed bag of textile material containing a soaking agent, and a portable open container. The soaking solution is 0.6g. +0.8g trehalose + 10mg azoxystrobin + 0.2g chitosan oligosaccharide + 25mg salicylic acid + 100mL water; Take 1-2 year old branches from superior wild garlic plants (superior plants refer to those that bear fruit early and produce a large quantity of fruit) in the wild. Immerse the 1-2 year old branches in an open container filled with the soaking solution, making a slanted cut to prevent resin from overflowing and sticking to the cut, and to reduce microbial infection of the branches through the cut. Soak for 25 minutes. The slanted cut combined with the appropriate soaking time allows the branches to fully absorb the active agent. After soaking, make a horizontal cut. The cut surface is then wrapped with textile material moistened with an active agent, and the textile material is then covered with a sealed bag. After the sealed bag is tightly sealed, it is transported to the cultivation base. The 1-2 year old branches are disinfected by soaking in a 600-fold carbendazim solution for 5 minutes. The disinfected branches are then revived and cut into 4 cm long sections. The sections are obliquely cut into a horse ear shape. A 1-2 cm deep vertical cut is made at the lower 1 / 3 of the horse ear shape to form a tongue-shaped structure, thus obtaining the scion.

[0044] Rootstock preparation: Select healthy, 2-3 year old artificially cultivated garlic bulbs as rootstock. For 14 days prior to grafting, continuously administer 25mg salicylic acid + 65mg boric acid + 0.6g... Add 1.5g trehalose, 0.2g chitosan oligosaccharide, and 100mL of water for root irrigation;

[0045] Grafting Method: The specific steps of smoothing the rootstock at a height of 16cm (flat cut or short cut), tongue-grafting the rootstock and scion, aligning the cuts of the scion and rootstock, and securing with grafting film are as follows: Smooth the rootstock at a height of 16cm, make a slanted cut in the shape of a horse's ear 8cm from the cut surface of the rootstock, and vertically cut a 1-2cm deep incision at the upper 1 / 3 of the slanted cut surface to form a tongue-like structure. Absorb the moisture from the scion, and make an incision at the grafting site on the scion. After aligning the cuts of the scion and rootstock with the same cut, tie the grafting film tightly. Apply the healing agent to the smoothed (flat or shortened) cut of the rootstock, and also to the top of the grafting film. This serves two purposes: first, to allow the healing agent to flow from the top of the grafting film into the graft union, promoting healing; and second, to improve the sealing of the top of the grafting film after the healing agent forms a film. The healing agent consists of 1.5g sodium alginate + 65mg NAA + 8g activated charcoal + 1g PVP + 100mL water.

[0046] Post-grafting management: Control the cultivation temperature at 27-30℃ and the cultivation humidity at 75-85%. After the graft has taken, promptly remove any newly sprouted buds from the rootstock. For rootstocks that show no signs of sprouting after 30 days, regraft them promptly. When the scion is longer than 10cm, begin applying a water-soluble fertilizer once a month, consisting of 18mg / L potassium sulfate, 12mg / L potassium dihydrogen phosphate, 6mg / L magnesium sulfate, 8mg / L ferrous sulfate, 4mg / L zinc sulfate, and 5mg / L sodium alginate. Example 2

[0047] A method for improving the grafting survival rate of garlic cloves, the method comprising:

[0048] S1. Scion Preparation: When collecting scions in the wild, carry an appropriate amount of soaking solution, a sealed bag containing gauze soaked with activator, and a portable open container. Collect 1-2 year old branches from healthy, vigorous, perennial wild garlic scapes. Immerse them in the open container containing the soaking solution and make oblique cuts. After soaking in the solution for 20-60 minutes, make horizontal cuts directly in the solution. Remove the gauze and wrap the horizontal cut of the branch tightly. Then put it back into the sealed bag and tie the bag tightly before transporting it to the cultivation base. Upon arrival, immediately rinse the 1-2 year old branches with running water and soak them in a 600-fold diluted carbendazim solution for 5 minutes to obtain disinfected branches. The soaking solution contains 0.5-0.8g of scion. +0.2-1g trehalose + 5-13mg azoxystrobin + 0.1-0.3g chitosan oligosaccharide + 20-30mg salicylic acid + 100mL water;

[0049] S2, Rootstock Preparation: Select robust 2-3 year old artificially cultivated garlic-shaped fruit as rootstock. For 14 days before grafting, continuously administer 20-30mg salicylic acid + 60-80mg boric acid + 0.5-0.8g of [unspecified substance]. A mixture of 1-3g trehalose, 0.1-0.3g chitosan oligosaccharide, and 100mL water is used for root irrigation.

[0050] S3, grafting method is as follows:

[0051] S31, Prepare scions using the disinfected branches: Resurrect the disinfected branches, cut the resurrected branches into branches that are 4-8cm long, cut them obliquely into a horse ear-shaped cut, and make a 1-2cm deep vertical cut at the lower 1 / 3 of the horse ear-shaped cut to form a tongue-shaped structure to obtain scions.

[0052] S32, cut the rootstock short (smooth or flat) at a height of 15-30cm, make a horse ear-shaped cut at a distance of 8-10cm from the cross-section of the rootstock, and make a 1-2cm deep vertical cut at the upper 1 / 3 of the horse ear-shaped cut to form a tongue-shaped structure.

[0053] S33, after aligning the scion with the rootstock cut, tie the branch and rootstock tightly with grafting film, and apply the healing agent to the cut of the shortened (shaving or flat cut) rootstock and the upper end of the grafting film. The healing agent is 1-3g sodium alginate + 50-70mg NAA + 7-10g activated carbon + 0.5-1g PVP + 100mL water.

[0054] S4. Post-grafting management: Observe the growth of the scion branches and the condition of the grafting point;

[0055] When the scion reaches 10cm in length, begin applying a water-soluble fertilizer once a month, consisting of 15-20mg / L potassium sulfate, 15-20mg / L potassium dihydrogen phosphate, 4-6mg / L magnesium sulfate, 6-10mg / L ferrous sulfate, 3-5mg / L zinc sulfate, and 1-5mg / L sodium alginate. Example 3

[0056] A method for improving the grafting survival rate of garlic cloves, the method comprising:

[0057] S1, Scion Preparation: When collecting materials in the wild, carry an appropriate amount of soaking solution, a sealed bag containing gauze soaked with activator, and a portable open container. Collect 1-2 year old branches from healthy, vigorous, perennial wild garlic plants. Soak the branches in the open container containing the soaking solution and cut them obliquely. After soaking in the solution for 25 minutes, cut them horizontally directly in the solution. Remove the gauze and wrap the cut ends of the branches tightly. Put them back into the sealed bag and tie the bag tightly before transporting them to the cultivation base. Upon arrival, rinse the 1-2 year old branches with running water and soak them in a 600-fold carbendazim solution for 5 minutes to obtain disinfected branches. The soaking solution consists of 0.6g CaCl2 + 0.8g trehalose + 10mg azoxystrobin + 0.2g chitosan oligosaccharide + 25mg salicylic acid + 100mL water.

[0058] S2, Rootstock preparation: Select healthy 2-3 year old artificially cultivated garlic fruit as rootstock. For 14 days before grafting, continuously drench the roots with a mixture of 25mg salicylic acid + 65mg boric acid + 0.6g CaCl2 + 1.5g trehalose + 0.2g chitosan oligosaccharide + 100mL water.

[0059] S3, grafting method is as follows:

[0060] S31, preparing scions using the disinfected branches: cut off the cut ends of the disinfected branches, then insert the cut branches into the activator for 3-4 hours to allow the branches to fully absorb the activator. Cut the revived branches into 5cm long branches, and make oblique cuts into a horse ear-shaped surface. Make a 1-2cm deep vertical cut at the lower 1 / 3 of the horse ear-shaped surface to form a tongue-shaped structure to obtain the scion. The activator is 20mg glycine + 20mg glutamic acid + 25mg betaine + 8mg sterol + 65mg trehalose + 12mg glutathione + 65mg NAA + 100mL water.

[0061] S32, make a horizontal cut at a height of 16cm on the rootstock, make a slanted cut at a distance of 8cm from the cross-section of the rootstock to form a horse ear-shaped surface, and make a vertical cut 1-2cm deep at the upper 1 / 3 of the horse ear-shaped surface to form a tongue-shaped structure.

[0062] S33, after aligning the scion with the rootstock cut, tie the branch and rootstock tightly with grafting film, and apply the healing agent to the cut of the rootstock and the upper end of the grafting film. The healing agent is 1.5g sodium alginate + 65mg NAA + 8g activated carbon + 1g PVP + 100mL water.

[0063] S4. Post-grafting management: Observe the growth of the scion branches and the condition of the grafting point;

[0064] When the scion reaches 10cm in length, begin applying a water-soluble fertilizer once a month, consisting of 18mg / L potassium sulfate, 12mg / L potassium dihydrogen phosphate, 6mg / L magnesium sulfate, 8mg / L ferrous sulfate, 4mg / L zinc sulfate, and 5mg / L sodium alginate. Example 4

[0065] A method for improving the grafting survival rate of garlic cloves, the method comprising:

[0066] S1, Scion Preparation: When collecting materials in the wild, carry an appropriate amount of soaking solution, a sealed bag containing gauze soaked with activator, and a portable open container. Collect 1-2 year old branches from healthy, vigorous, perennial wild garlic plants. Soak the branches in the open container containing the soaking solution and cut them obliquely. After soaking in the solution for 25 minutes, cut them horizontally directly in the solution. Remove the gauze and wrap the cut ends of the branches tightly. Put them back into the sealed bag and tie the bag tightly before transporting them to the cultivation base. After arrival, rinse the 1-2 year old branches with running water and soak them in a 600-fold carbendazim solution for 5 minutes to obtain disinfected branches. The soaking solution consists of 0.8g CaCl2 + 0.3g trehalose + 13mg azoxystrobin + 0.1g chitosan oligosaccharide + 20mg salicylic acid + 100mL water.

[0067] S2, Rootstock preparation: Select healthy 2-3 year old artificially cultivated garlic fruit as rootstock. For 14 days before grafting, continuously drench the roots with a mixture of 20mg salicylic acid + 75mg boric acid + 0.8g CaCl2 + 1g trehalose + 0.3g chitosan oligosaccharide + 100mL water.

[0068] S3, grafting method is as follows:

[0069] S31, after disinfecting the branches, cut off the cut ends, then insert the cut branches into the activator for 3-4 hours to allow the branches to fully absorb the activator. Cut the revived branches into 6cm long branches, and make oblique cuts into a horse ear-shaped surface. Make a 1-2cm deep vertical cut at the lower 1 / 3 of the horse ear-shaped surface to form a tongue-shaped structure to obtain the scion. The activator is 15mg glycine + 15mg glutamic acid + 20mg betaine + 6mg sterol + 60mg trehalose + 8mg glutathione + 65mg NAA + 100mL water.

[0070] S32, cut the rootstock short at a height of 18cm, make a horse ear-shaped cut at a distance of 10cm from the cross-section of the rootstock, and make a 1-2cm deep vertical cut at the upper 1 / 3 of the horse ear-shaped cut to form a tongue-shaped structure.

[0071] S33, after aligning the scion with the rootstock cut, tie the branch and rootstock tightly with grafting film, and apply the healing agent to the cut of the pruned rootstock and the upper end of the grafting film. The healing agent is 3g sodium alginate + 50mg NAA + 10g activated carbon + 1g PVP + 100mL water.

[0072] S4. Post-grafting management: Observe the growth of the scion branches and the condition of the grafting point;

[0073] When the scion reaches 10cm in length, begin applying a water-soluble fertilizer once a month, consisting of 15mg / L potassium sulfate, 15mg / L potassium dihydrogen phosphate, 4mg / L magnesium sulfate, 6mg / L ferrous sulfate, 5mg / L zinc sulfate, and 1mg / L sodium alginate. Comparative Example 1

[0074] A method for grafting garlic cloves, the method comprising:

[0075] S1, Scion Preparation: Take 1-2 year old branches from healthy, vigorous, perennial wild garlic bulb plants from the wild. Soak them obliquely in an open container filled with a soaking solution. Then, use 0.6g of... Soak the branch in a solution consisting of 0.8g trehalose, 10mg azoxystrobin, 0.2g chitosan oligosaccharide, 25mg salicylic acid, and 100mL water for 25 minutes. Then, cut the branch directly in the solution. Wrap the cut end of the branch with gauze, put it back into a sealed bag and tie the bag tightly before transporting it to the cultivation base. Rinse the branch with running water and soak it in a 600-fold carbendazim solution for 5 minutes. Retain one pre-bud point. Make a slanted cut below the pre-bud point, with one cut surface 1.5cm long and another cut surface 0.6cm long. The depth of the two cut surfaces should be just halfway to the xylem. Make a horizontal cut 2cm above the pre-bud point to obtain the scion. Place the scion in distilled water and stir to clean it.

[0076] S2, Rootstock Preparation: Select robust 2-3 year old artificially cultivated garlic-shaped fruit as rootstock. For 14 days prior to grafting, continuously administer 25mg salicylic acid + 65mg boric acid + 0.6g... A mixture of 1.5g trehalose, 0.2g chitosan oligosaccharide, and 100mL water was used for root irrigation.

[0077] S3, Grafting method: Shave the rootstock at a height of 16cm, cut open the lower epidermis of the rootstock cross-section to a depth just reaching the xylem, making a 1.5cm long incision. Align the cambium layer of the scion on at least one side with the cambium layer of the rootstock, and tie it tightly with grafting film. Cover the rootstock cross-section with the grafting film. Apply the healing agent to the cut of the rootstock. The healing agent is 1.5g sodium alginate + 65mg NAA + 8g activated charcoal + 1g PVP + 100mL water. Comparative Example 2

[0078] A method for grafting garlic cloves, the method comprising:

[0079] S1, Scion preparation: Take 1-2 year old branches from vigorous, perennial wild garlic plants in the wild. Take out the gauze soaked in distilled water and wrap the cut end of the branch tightly. Put it back into the sealed bag and tie the bag tightly before transporting it to the cultivation base. Rinse the branch with running water and soak it in a 600-fold carbendazim solution for 5 minutes. Keep an 8cm long branch and cut it obliquely into a horse ear shape. Make a 1-2cm deep vertical cut at the lower 1 / 3 of the horse ear shape to form a tongue-shaped structure to obtain the scion.

[0080] S2, Rootstock Preparation: Select robust 2-3 year old artificially cultivated garlic-shaped fruit as rootstock. For 14 days prior to grafting, continuously administer 25mg salicylic acid + 65mg boric acid + 0.6g... A mixture of 1.5g trehalose, 0.2g chitosan oligosaccharide, and 100mL water was used for root irrigation.

[0081] S3, grafting method is as follows:

[0082] S31, preparing scions using the disinfected branches: cut off the cut ends of the disinfected branches, then insert the cut branches into the activator for 3-4 hours to allow the branches to fully absorb the activator. Cut the revived branches into 5cm long branches, and make oblique cuts into a horse ear-shaped surface. Make a 1-2cm deep vertical cut at the lower 1 / 3 of the horse ear-shaped surface to form a tongue-shaped structure to obtain the scion. The activator is 20mg glycine + 20mg glutamic acid + 25mg betaine + 8mg sterol + 65mg trehalose + 12mg glutathione + 65mg NAA + 100mL water.

[0083] S32, make a horizontal cut at a height of 16cm on the rootstock, make a slanted cut at a distance of 8cm from the cross-section of the rootstock to form a horse ear-shaped surface, and make a vertical cut 1-2cm deep at the upper 1 / 3 of the horse ear-shaped surface to form a tongue-shaped structure.

[0084] S33, after aligning the scion with the rootstock cut, tie the branch and rootstock tightly with grafting film, and apply the healing agent to the cut of the rootstock and the upper end of the grafting film. The healing agent is 1.5g sodium alginate + 65mg NAA + 8g activated carbon + 1g PVP + 100mL water. Comparative Example 3

[0085] A method for grafting garlic cloves, the method comprising:

[0086] S1, Scion Preparation: Take 1-2 year old branches from healthy, vigorous, perennial wild garlic bulb plants from the wild. Immerse them in an open container filled with a soaking solution, making oblique cuts. After soaking for 25 minutes, make horizontal cuts directly in the solution. Wrap the cut ends of the branches tightly with gauze, then place them back into a sealed bag and tie the bag tightly before transporting them to the cultivation base. Immediately afterward, rinse the 1-2 year old branches with running water and soak them in a 600-fold diluted carbendazim solution for 5 minutes to obtain disinfected branches. The soaking solution contains 0.6g of carbendazim. +0.8g trehalose + 10mg azoxystrobin + 0.2g chitosan oligosaccharide + 25mg salicylic acid + 100mL water;

[0087] S2, Rootstock preparation: Select healthy 2-3 year old artificially cultivated garlic fruit as rootstock. For 14 days before grafting, continuously drench the roots with a mixture of 25mg salicylic acid + 1.5g trehalose + 100mL water.

[0088] S3, Grafting method:

[0089] S31, preparing scions using the disinfected branches: cut off the cut ends of the disinfected branches, then insert the cut branches into the activator for 3-4 hours to allow the branches to fully absorb the activator. Cut the revived branches into 5cm long branches, and make oblique cuts into a horse ear-shaped surface. Make a 1-2cm deep vertical cut at the lower 1 / 3 of the horse ear-shaped surface to form a tongue-shaped structure to obtain the scion. The activator is 20mg glycine + 20mg glutamic acid + 25mg betaine + 65mg trehalose + 12mg ascorbic acid + 65mg NAA + 100mL water.

[0090] S32, cut the rootstock short at a height of 16cm, make a slanted cut in the shape of a horse ear at a distance of 8cm from the cross-section of the rootstock, and make a vertical cut 1-2cm deep at the upper 1 / 3 of the horse ear-shaped cut to form a tongue-shaped structure.

[0091] S33, after aligning the scion with the rootstock cut, tie the branch and rootstock tightly with grafting film, and apply the healing agent to the cut of the shortened rootstock and the upper end of the grafting film. The healing agent is 1.5g sodium alginate + 65mg NAA + 8g activated carbon + 1g PVP + 100mL water. Comparative Example 4

[0092] A method for grafting garlic cloves, the method comprising:

[0093] S1, Scion Preparation: Take 1-2 year old branches from healthy, vigorous, perennial wild garlic bulb plants from the wild. Immerse them in an open container filled with a soaking solution, making oblique cuts. After soaking for 25 minutes, make horizontal cuts directly in the solution. Wrap the horizontal cut of the branch tightly with gauze, then place it back into a sealed bag and tie the bag tightly before transporting it to the cultivation base. Upon arrival, immediately rinse the 1-2 year old branches with running water and soak them in a 600-fold diluted carbendazim solution for 5 minutes to obtain disinfected branches. The soaking solution contains 0.6g of carbendazim. +0.8g trehalose + 10mg azoxystrobin + 0.2g chitosan oligosaccharide + 25mg salicylic acid + 100mL water;

[0094] S2, Rootstock preparation: Select healthy 2-3 year old artificially cultivated garlic fruits as rootstock;

[0095] S3, Grafting method:

[0096] S31, preparing scions using the disinfected branches: cut off the cut ends of the disinfected branches, then insert the cut branches into the activator for 3-4 hours to allow the branches to fully absorb the activator. Cut the revived branches into 5cm long branches, and make oblique cuts into a horse ear-shaped surface. Make a 1-2cm deep vertical cut at the lower 1 / 3 of the horse ear-shaped surface to form a tongue-shaped structure to obtain scions. The activator is 65mg NAA + 100mL water.

[0097] S32, make a horizontal cut at a height of 16cm on the rootstock, make a slanted cut at a distance of 8cm from the cross-section of the rootstock to form a horse ear-shaped surface, and make a vertical cut 1-2cm deep at the upper 1 / 3 of the horse ear-shaped surface to form a tongue-shaped structure.

[0098] S33, after aligning the scion with the rootstock cut, tie the branch and rootstock tightly with grafting film, and apply the healing agent to the cut of the rootstock and the upper end of the grafting film. The healing agent is 1.5g sodium alginate + 65mg NAA + 8g activated carbon + 1g PVP + 100mL water. Comparative Example 5

[0099] A method for grafting garlic cloves, the method comprising:

[0100] S1, Scion preparation: Take 1-2 year old branches from vigorous perennial wild garlic plants in the wild, immerse them in an open container filled with soaking solution, cut them obliquely, soak for 25 minutes, then cut the branches horizontally and transport them to the cultivation base; after the transport, rinse the 1-2 year old branches with running water and soak them in a 600-fold carbendazim solution for 5 minutes to obtain disinfected branches;

[0101] S2, Rootstock Preparation: Select robust 2-3 year old artificially cultivated garlic-shaped fruit as rootstock. For 14 days prior to grafting, continuously administer 25mg salicylic acid + 65mg boric acid + 0.6g... Spray the rootstock leaves with 1.5g trehalose, 0.2g chitosan oligosaccharide, and 100mL water.

[0102] S3, Grafting method:

[0103] S31, preparing scions using the disinfected branches: cut off the cut ends of the disinfected branches, then insert the cut branches into the activator for 3-4 hours to allow the branches to fully absorb the activator. Cut the revived branches into 5cm long branches, and make oblique cuts into a horse ear-shaped surface. Make a 1-2cm deep vertical cut at the lower 1 / 3 of the horse ear-shaped surface to form a tongue-shaped structure to obtain the scion. The activator is 25mg betaine + 65mg trehalose + 12mg glutathione + 65mg NAA + 100mL water.

[0104] S32, make a horizontal cut at a height of 16cm on the rootstock, make a slanted cut at a distance of 8cm from the cross-section of the rootstock to form a horse ear-shaped surface, and make a vertical cut 1-2cm deep at the upper 1 / 3 of the horse ear-shaped surface to form a tongue-shaped structure.

[0105] S33, after aligning the scion with the rootstock cut, tie the branch and rootstock tightly with grafting film, and apply the healing agent to the cut of the rootstock and the upper end of the grafting film. The healing agent is 1.5g sodium alginate + 65mg NAA + 8g activated carbon + 1g PVP + 100mL water.

[0106] S4. Post-grafting management: Observe the growth of the scion branches and the condition of the graft union.

[0107] Cellular dehydrogenase activity and relative water content of 1-2 year old branch stem segments from Examples 3, 4, Comparative Examples 1, 2, 3, 4, and 5 were randomly collected. The cellular dehydrogenase activity value was determined by measuring the cellular dehydrogenase activity after grinding the collected 1-2 year old branch stem segments. Cellular dehydrogenase activity was measured by grinding and testing healthy 1-2 year old garlic shoots from the cultivation base. Cellular dehydrogenase activity was measured after grinding healthy 1-2 year old garlic fruit stem segments boiled at 100℃ for 10 minutes in the cultivation base. , The relative moisture content is defined as W, which is the weight of 1-2 year old branch stem segments, dried in an oven at 75°C until constant weight is achieved. , The results are shown in Table 2.

[0108] Table 2 Relative water content and activity value

[0109]

[0110] Grafting was performed using the above grafting methods. If new leaves or new buds sprouted on the scion after grafting, it was defined as a successful graft. The survival rate was calculated and shown in Table 3 below.

[0111] Table 3 Grafting survival rate of each group

[0112]

[0113] Each group observes 20 plants at any time, and records the number of plants and the time when the scion grows to 10cm. The average growth time is calculated by adding up all the times and dividing by the number of plants, as shown in Table 4.

[0114] Table 4. Growth rate of scions

[0115]

[0116] As can be seen from Tables 3 and 4, the time required for scions and buds to grow to the same height is different. The time required for scions to grow to 10cm is much shorter than that of buds in Comparative Example 1, which can shorten the cultivation time of grafted seedlings. Furthermore, the grafting effect of the scion and rootstock using the method of this invention is good, and the grafted seedlings obtained have large leaves, robust overall plant growth, and retain the advantages of early fruiting and large fruit yield of superior plants.

[0117] This invention can be implemented in various ways and is not limited to the embodiments described. Those skilled in the art will understand that the invention can be implemented in other specific ways without changing the technical concept or essential features. Therefore, it should be understood that the embodiments described above are exemplary and not intended to limit the invention.

Claims

1. A method for improving the survival rate of grafted garlic cloves, characterized in that, include: S1, Scion preparation: Take branches from superior perennial wild garlic plants in the wild, immerse the branches in the soaking solution and cut them obliquely. After soaking for 20-60 minutes, cut them horizontally in the soaking solution. Wrap the horizontal cut with textile material moistened with the soaking solution and transport it to the inoculation site for disinfection. Obtain the disinfected branches. The components of the soaking solution include CaCl2, trehalose, azoxystrobin, chitosan oligosaccharide and salicylic acid. S2, Rootstock preparation: Select artificially cultivated garlic fruit as rootstock, and continuously drench the roots with a agent before grafting. The agent includes salicylic acid, boric acid, CaCl2 and sodium alginate. S3, grafting method: prepare scions using the disinfected branches, treat the rootstock, tongue-graft the rootstock and scion and tie them tightly with grafting film, and apply healing agent to the cut of the polished rootstock and the upper end of the grafting film. S4, post-grafting management.

2. The method for improving the grafting survival rate of garlic cloves as described in claim 1, characterized in that, In step S1, the soaking solution is 0.5-0.8g CaCl2 + 0.2-1g trehalose + 5-13mg azoxystrobin + 0.1-0.3g chitosan oligosaccharide + 20-30mg salicylic acid + 100mL water.

3. The method for improving the grafting survival rate of garlic cloves as described in claim 1, characterized in that, In step S2, the reagent is 20-30mg salicylic acid + 60-80mg boric acid + 0.5-0.8g CaCl2 + 1-3g trehalose + 0.1-0.3g chitosan oligosaccharide + 100mL water.

4. The method for improving the grafting survival rate of garlic cloves as described in claim 1, characterized in that, In step S2, the rootstock is a healthy 2-3 year old artificially cultivated garlic fruit.

5. The method for improving the grafting survival rate of garlic cloves as described in claim 1, characterized in that, The specific steps of the grafting method described in step S3 are as follows: S31, Prepare scions using the disinfected branches: Resurrect the disinfected branches, cut the resurrected branches into branches that are 4-8cm long, cut them obliquely into a horse ear-shaped cut, and make a 1-2cm deep vertical cut at the lower 1 / 3 of the horse ear-shaped cut to form a tongue-shaped structure to obtain scions. S32, cut the rootstock short at a height of 15-30cm, make a slanted cut in the shape of a horse ear at a distance of 8-10cm from the cross-section of the rootstock, and make a vertical cut 1-2cm deep at the upper 1 / 3 of the horse ear-shaped cut to form a tongue-shaped structure. S33, after aligning the scion with the rootstock cut, bind the branch and rootstock tightly with grafting film, and apply healing agent to the cut of the polished rootstock and the upper end of the grafting film.

6. The method for improving the grafting survival rate of garlic shoots as described in claim 5, characterized in that, The healing agent is 1-3g sodium alginate + 50-70mg NAA + 7-10g activated charcoal + 0.5-1g PVP + 100mL water.

7. The method for improving the grafting survival rate of garlic cloves as described in claim 5, characterized in that, The process of reviving the disinfected branches involves cutting off the cut ends of the disinfected branches and then inserting the cut branches into an activator for 3-4 hours to allow the branches to fully absorb the activator.

8. The method for improving the grafting survival rate of garlic cloves as described in claim 7, characterized in that, The active agent is 10-20 mg glycine + 10-20 mg glutamic acid + 20-30 mg betaine + 5-10 mg sterol + 60-70 mg trehalose + 5-15 mg glutathione + 50-70 mg NAA + 100 mL water.

9. The method for improving the grafting survival rate of garlic cloves as described in claim 1, characterized in that, The specific post-grafting treatment is as follows: control the cultivation temperature at 27-30℃ and the cultivation humidity at 75-85%, promptly remove newly sprouted buds from the rootstock, and promptly regraft rootstocks that show no signs of sprouting after 30 days; when the scion length is ≥10cm, start applying water and fertilizer 1-2 times per month.

10. The method for improving the grafting survival rate of garlic cloves as described in claim 9, characterized in that, The ferrous sulfate solution is made from 15-20 mg / L potassium sulfate, 15-20 mg / L potassium dihydrogen phosphate, 4-6 mg / L magnesium sulfate, 6-10 mg / L ferrous sulfate, 3-5 mg / L zinc sulfate, and 1-5 mg / L sodium alginate.

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