Low-calorie electrolyte beverage and method of making same

Abstract

The application discloses a kind of low-carb electrolyte beverage and preparation method thereof in the technical field of beverage, by the following weight parts of component composition: sida acuta extract 1.3-1.5 parts, vitamin C 0.02-0.03 parts, vitamin B2 0.016-0.019 parts, citric acid 0.15-0.25 parts, sodium citrate 0.1-0.2 parts, sodium chloride 0.02-0.03 parts, potassium chloride 0.02-0.03 parts, stevia sugar 0.01-0.02 parts and deionized water 150-180 parts.The application proposes to add sida acuta soluble fiber to provide osmotic pressure, achieve sugar substitution, use sugar substitute to adjust taste, and extract sida acuta peptide, which jointly act to improve water absorption efficiency after exercise, and further improve the effect of water supplementation.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of beverages, and particularly relates to a low-calorie electrolyte beverage and a preparation method thereof. BACKGROUND

[0002] Water in the human body maintains a dynamic balance state, when the body intakes too little water or loses too much water, the body will be in a state of dehydration, under high-intensity exercise, drinking water can improve the performance of circulatory endurance exercise, and the osmotic pressure of the beverage can affect the speed of gastric emptying and thus affect the rate of water absorption in the intestinal tract, therefore, current sports beverages are mostly mixed with multiple carbohydrates to ensure the palatability and rehydration effect of the sports beverages; there are also sports beverages that add peptides, amino acids or proteins on the basis of carbohydrates and electrolytes, amino acids and proteins can also be transported in combination with sodium to increase water absorption in the intestinal tract, but excessive intake of sugar often leads to elevated blood glucose and weight gain, high sodium and high carbohydrate intake are closely related to increased risk of hypertension and cardiovascular disease; the Opuntia tuna contains rich unsaturated fatty acids, proteins and polyphenols and other chemical components, and modern pharmacological research shows that it has the effects of lowering blood pressure, lowering blood lipids, improving immunity and improving memory.

[0003] The current prior art mainly has the following problems: 1. The current electrolyte rehydration beverage has a high sugar content; 2. The sugar-free low-calorie beverage has poor rehydration effect. SUMMARY

[0004] In view of the above situation, in order to overcome the defects of the prior art, the application provides a low-calorie electrolyte beverage and a preparation method thereof, in order to solve the problem of high content of electrolyte beverages, the application proposes a method of adding Opuntia tuna soluble fiber to provide osmotic pressure, realizing sugar substitution, using sugar substitutes to adjust the taste, and extracting Opuntia tuna peptides to jointly improve the water absorption efficiency after exercise, thereby improving the rehydration effect.

[0005] In order to achieve the above purpose, the technical scheme adopted by the application is as follows: the application provides a low-calorie electrolyte beverage, the low-calorie electrolyte beverage is composed of the following components in parts by weight: Opuntia tuna extract 1.3-1.5 parts, vitamin C 0.02-0.03 parts, vitamin B2 0.016-0.019 parts, citric acid 0.15-0.25 parts, sodium citrate 0.1-0.2 parts, sodium chloride 0.02-0.03 parts, potassium chloride 0.02-0.03 parts, stevia sugar 0.01-0.02 parts and deionized water 150-180 parts.

[0006] Preferably, the preparation method of the Opuntia tuna extract specifically comprises the following steps:

[0007] S1, dry, powder, and pass the 60 mesh sieve of the macerate of the S. febrifugus, add to the 1.3wt% sodium chloride solution, then add glucose, mix well, and cook under normal pressure for 20-30min to obtain the fermentation substrate;

[0008] S2, transfer the fermentation substrate obtained in S1 to a sealed fermentation tank, inoculate with the I. orientalis, ferment at 28℃ for 10h, inoculate with the C. cellulolyticum, increase the temperature to 35℃, and shake at 120rpm for 30h, finally store at 4℃ for 6h to obtain the fermentation product;

[0009] S3, pulp the fermentation product obtained in S2 by a pulper, filter through a 150 mesh sieve to obtain the filtrate and the residue, centrifuge the filtrate for 10min, take the supernatant, and freeze-dry to obtain the S. febrifugus peptide;

[0010] S4, add the residue obtained in S3 to the 3wt% sulfuric acid solution, stir in a water bath, centrifuge for 20min, and take the supernatant;

[0011] S5, add 3-4 times the volume of anhydrous ethanol to the supernatant obtained in S4, centrifuge for 6min after 16h to take the precipitate, wash, freeze-dry to obtain the S. febrifugus soluble fiber, add the S. febrifugus peptide obtained in S3, mix well to obtain the S. febrifugus extract.

[0012] Preferably, in S1, the addition amount of the S. febrifugus macerate in the 1.3wt% sodium chloride solution is 0.5-0.6g / mL.

[0013] Preferably, in S1, the addition amount of glucose is 6-8mg / mL.

[0014] Preferably, in S2, the inoculation amount of the I. orientalis is 2-3%; the biological source is the China Industrial Microbial Culture Collection Center, and the strain number is CICC32637.

[0015] Preferably, in S2, the inoculation amount of the C. cellulolyticum is 2-4%; the biological source is the China Industrial Microbial Culture Collection Center, and the strain number is CICC8022.

[0016] The I. orientalis and the C. cellulolyticum are used in combination, the I. orientalis is used for preliminary fermentation, and the C. cellulolyticum is used for middle and late stage fermentation; the preliminary fermentation uses glucose as the substrate, the aerobic respiration of the I. orientalis reduces the oxygen content of the fermentation tank and destroys the cell wall, providing favorable conditions for the fermentation of the C. cellulolyticum, reducing the maximum acid production time of the C. cellulolyticum, promoting the release of effective ingredients, and improving the overall fermentation effect.

[0017] Preferably, in S3, the centrifugation speed is 3600-4200rpm.

[0018] Preferably, in S4, the addition amount of the filter residue in the 3wt% sulfuric acid solution is 0.05-0.06g / mL.

[0019] Preferably, in S4, the water bath stirring is at a temperature of 60-70℃, a speed of 80-120rpm, and a time of 2-3h.

[0020] Preferably, in S4, the centrifugal speed is 3000-4000rpm.

[0021] Preferably, in S5, the centrifugal speed is 2500-3000rpm.

[0022] The application also provides a preparation method of a low-calorie electrolyte beverage, specifically comprising the following steps:

[0023] Sodium chloride and potassium chloride are added to deionized water, stirred and dissolved, and then vitamin C, vitamin B2, citric acid, sodium citrate, Monacolin, and stevioside are added, stirred and dissolved, filtered, sterilized, and a low-calorie electrolyte beverage is obtained.

[0024] The application has the following beneficial effects: the application delays gastric emptying rate by adding Monacolin soluble fiber and Monacolin peptide, thereby delaying intestinal absorption, increasing electrolytes to increase body water retention, thereby enhancing kidney water reabsorption, reducing urine, and increasing body fluid retention; on the other hand, Monacolin soluble fiber has good in vitro antioxidant activity and immune activity, the use of Monacolin peptide can reduce body fluid loss, help increase water and electrolyte recovery, improve cell fluid replacement speed, and thereby delay fatigue; Monacolin meal contains a high content of essential amino acids, among which methionine and tyrosine are particularly rich, has high nutritional value, is beneficial to preventing muscle decomposition after exercise, promotes muscle protein synthesis, increases muscle mass, and improves physical recovery speed. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 Fig. 1 is a result graph of water supplement effect test of the application examples 1-3 and the comparative examples 1-2,

[0026] Figure 2 Fig. 2 is a result graph of body fluid loss test of the application examples 1-3 and the comparative examples 1-2,

[0027] Figure 3 Fig. 3 is a result graph of exercise capacity test of the application examples 1-3 and the comparative examples 1-2.

[0028] The accompanying drawings are included to provide a further understanding of the application, and constitute a part of the specification, illustrate the application, and are used to explain the application, and do not constitute a limitation on the application. DETAILED DESCRIPTION

[0029] With reference to the drawings of the embodiments of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments of the present application, all the other embodiments obtained by a person of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0030] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. In addition, any method and material similar or equivalent to those described herein can be used in the present application. The preferred methods and materials described herein are only for illustration, but cannot limit the content of the present application.

[0031] In the following examples, the experimental methods are all conventional methods unless otherwise specified. In the following examples, the test materials and test strains are all purchased from commercial channels unless otherwise specified. Embodiment

[0032] A low-calorie electrolyte beverage is composed of the following components by weight: 1.3 parts of Monodora myristica extract, 0.02 parts of vitamin C, 0.016 parts of vitamin B2, 0.15 parts of citric acid, 0.1 parts of sodium citrate, 0.02 parts of sodium chloride, 0.02 parts of potassium chloride, 0.01 parts of stevia sugar, and 150 parts of deionized water.

[0033] The preparation method of Monodora myristica extract specifically includes the following steps:

[0034] S1, dry Monodora myristica meal, powder, and pass through a 60-mesh sieve. Add 0.5 g / mL of the meal to a 1.3 wt% sodium chloride solution, then add 6 mg / mL of glucose, mix well, and cook under normal pressure for 20 min to obtain a fermentation substrate;

[0035] S2, transfer the fermentation substrate obtained in S1 to a sealed fermentation tank, inoculate with I. orientalis at an inoculation amount of 2%, and ferment at 28℃ for 10 h. Inoculate with Clostridium cosel at an inoculation amount of 2%, increase the temperature to 35℃, and shake at 120 rpm for 30 h. Finally, store at 4℃ for 6 h to obtain a fermentation product;

[0036] S3, pulp the fermentation product obtained in S2 with a pulper, filter with a 150-mesh sieve to obtain a filtrate and a residue. Centrifuge the filtrate at 3600 rpm for 10 min, take the supernatant, and freeze-dry to obtain Monodora myristica peptide;

[0037] S4, add the residue obtained in S3 to a 3 wt% sulfuric acid solution at an addition amount of 0.05 g / mL, stir at 60℃ for 2 h, centrifuge at 3000 rpm for 20 min, and take the supernatant;

[0038] S5, add 3 times the volume of absolute ethanol to the supernatant obtained in S4, centrifuge at 2500 rpm for 6 min after 16 h to obtain the precipitate, wash, freeze-dry to obtain soluble fiber of S. feofilivex, add the S. feofilivex peptide obtained in S3, and mix uniformly to obtain the S. feofilivex extract.

[0039] The application also provides a preparation method of a low-calorie electrolyte beverage, specifically comprising the following steps:

[0040] Sodium chloride and potassium chloride are added to deionized water, stirred and dissolved, vitamin C, vitamin B2, citric acid, sodium citrate, S. feofilivex extract, and stevia sugar are added, stirred and dissolved, filtered, sterilized, and a low-calorie electrolyte beverage is obtained. Embodiment

[0041] A low-calorie electrolyte beverage is composed of the following components by weight: 1.5 parts of S. feofilivex extract, 0.03 parts of vitamin C, 0.019 parts of vitamin B2, 0.25 parts of citric acid, 0.2 parts of sodium citrate, 0.03 parts of sodium chloride, 0.03 parts of potassium chloride, 0.02 parts of stevia sugar, and 180 parts of deionized water.

[0042] A preparation method of S. feofilivex extract, specifically comprising the following steps:

[0043] S1, dry the S. feofilivex meal, powder it, pass it through a 60-mesh sieve, add it to a 1.3wt% sodium chloride solution at an addition amount of 0.6g / mL, then add glucose at an addition amount of 8mg / mL, mix uniformly, and cook under normal pressure for 30 min to obtain a fermentation substrate;

[0044] S2, transfer the fermentation substrate obtained in S1 to a sealed fermentation tank, inoculate it with I. orientalis, the inoculation amount of I. orientalis is 3%, ferment at 28℃ for 10 h, inoculate it with C. cellulolyticum, the inoculation amount of C. cellulolyticum is 4%, increase the temperature to 35℃, and shake at 120 rpm for 30 h, and finally store it at 4℃ for 6 h to obtain a fermentation product;

[0045] S3, pulp the fermentation product obtained in S2 with a pulper, filter it through a 150-mesh sieve to obtain a filtrate and a residue, centrifuge the filtrate at 4200 rpm for 10 min to obtain a supernatant, and freeze-dry it to obtain S. feofilivex peptide;

[0046] S4, add the residue obtained in S3 to a 3wt% sulfuric acid solution at an addition amount of 0.06g / mL, stir at 70℃ in a water bath for 3 h, centrifuge at 4000 rpm for 20 min, and take the supernatant;

[0047] S5, adding 4 times volume of anhydrous ethanol to the supernatant obtained in S4, centrifuging at 3000 rpm for 6 min after 16 h to obtain the precipitate, washing, freeze-drying to obtain soluble fiber of S. feofilivivii, adding the S. feofilivivii peptide obtained in S3, and uniformly mixing to obtain the S. feofilivivii extract.

[0048] The application further provides a preparation method of the low-calorie electrolyte beverage, and specifically comprises the following steps:

[0049] Sodium chloride and potassium chloride are added to deionized water, stirred and dissolved, vitamin C, vitamin B2, citric acid, sodium citrate, S. feofilivivii extract, and stevia sugar are added, stirred and dissolved, filtered, sterilized, and a low-calorie electrolyte beverage is obtained. Embodiment

[0050] A low-calorie electrolyte beverage is composed of the following components by weight: 1.4 parts of S. feofilivivii extract, 0.025 parts of vitamin C, 0.018 parts of vitamin B2, 0.2 parts of citric acid, 0.15 parts of sodium citrate, 0.025 parts of sodium chloride, 0.025 parts of potassium chloride, 0.015 parts of stevia sugar, and 16 parts of deionized water.

[0051] The application further provides a preparation method of the S. feofilivivii extract, and specifically comprises the following steps:

[0052] S1, S. feofilivivii meal is dried, powdered, and filtered through a 60-mesh sieve, and then added to a 1.3wt% sodium chloride solution at an addition amount of 0.55 g / mL, followed by adding glucose at an addition amount of 7 mg / mL, uniformly mixing, and cooking under normal pressure for 25 min to obtain a fermentation substrate;

[0053] S2, the fermentation substrate obtained in S1 is transferred to a sealed fermentation tank, inoculated with East Is a yeast, the inoculation amount of the East Is a yeast is 2.5%, and then fermented at 28℃ for 10 h, inoculated with Clostridium cosel, the inoculation amount of the Clostridium cosel is 3%, the temperature is increased to 35℃, and then fermented at 120 rpm for 30 h, and finally stored at 4℃ for 6 h to obtain a fermentation product;

[0054] S3, the fermentation product obtained in S2 is beaten by a beater, filtered through a 150-mesh sieve to obtain a filtrate and a residue, the filtrate is centrifuged at 3900 rpm for 10 min to obtain a supernatant, and then freeze-dried to obtain a S. feofilivivii peptide;

[0055] S4, the residue obtained in S3 is added to a 3wt% sulfuric acid solution at an addition amount of 0.055 g / mL, stirred at 100 rpm in a 65℃ water bath for 2.5 h, centrifuged at 3500 rpm for 20 min, and then the supernatant is obtained;

[0056] S5, 3.5 times the volume of anhydrous ethanol was added to the supernatant obtained in S4, and after 16 h, the precipitate was obtained by centrifugation at 2700 rpm for 6 min, washed, freeze-dried to obtain soluble fiber of S. feejeensis, and the S. feejeensis peptide obtained in S3 was added and mixed uniformly to obtain the S. feejeensis extract.

[0057] The application also provides a preparation method of a low-calorie electrolyte beverage, specifically comprising the following steps:

[0058] Sodium chloride and potassium chloride were added to deionized water, stirred and dissolved, and then vitamin C, vitamin B2, citric acid, sodium citrate, S. feejeensis extract, and stevia sugar were added and stirred and dissolved, filtered, and sterilized to obtain the low-calorie electrolyte beverage.

[0059] Comparative Example 1

[0060] This comparative example provides a beverage, which is different from Example 1 in that glucose is used to replace the soluble fiber of S. feejeensis in the components, and the remaining components and component contents are the same as those of Example 1.

[0061] Comparative Example 2

[0062] This comparative example provides a beverage, which is different from Example 1 in that the S. feejeensis peptide is not contained in the components, and the remaining components and component contents are the same as those of Example 1.

[0063] Experimental Example

[0064] After the rats were adaptively fed for 3 days, they were deprived of water for 16 h, and then they were made to exercise on an animal treadmill after the end of the water deprivation. The initial running speed was set to 10 m / min, and the speed was uniformly increased to 20 m / min within 10 min, and the rats were made to exercise for a total of 30 min to obtain the water deprivation model rats.

[0065] 1. Water replenishment effect test

[0066] The water deprivation model rats were randomly divided into 7 groups, 5 rats in each group, as model groups and experimental groups. The experimental groups were set as: a control group, Examples 1-3, and Comparative Examples 1-2. The model groups were not given gavage, and the experimental groups were given gavage of 10 mL of water replenishment liquid every 1 h. The control group was given gavage with pure water, and Examples 1-3 and Comparative Examples 1-2 were given gavage with the corresponding prepared beverages. After 4 times of gavage, the plasma osmotic pressure of each group was recorded, and the water replenishment recovery rate was calculated according to the following formula:

[0067] Water replenishment recovery rate = (osmotic pressure of model group - osmotic pressure of experimental group) / osmotic pressure of model group x 100%.

[0068] Figure 1The results of the water supplement effect test of the application examples 1-3 and the comparative examples 1-2 are shown in the figure, and the water supplement recovery rates of the control group, the application examples 1-3 and the comparative examples 1-2 are 10.36%, 24.33%, 21.36%, 22.53% and 16.62%, 17.23% respectively; it is illustrated that the use of the inulin peptides and the inulin soluble fiber can obviously improve the water supplement recovery of the rats.

[0069] 2. Body fluid loss test

[0070] The water-deficient model rats were randomly divided into 6 groups, 5 rats in each group, and the saliva samples were extracted and recorded 2 H2O content, recorded as base 2 H2O content, and the rats were supplemented with pure water, the application examples 1-3 and the comparative examples 1-2 respectively at a dose of 0.05 g / kg body weight, and each group of solutions contained 12% of 2 H2O, and the saliva samples were extracted again 1h after the supplementation, and recorded 2 H2O content, recorded as test 2 H2O content; after the supplementation, the rats were subjected to a 30min, 10m / min exercise, and then the water was cut off for 3h, and recorded 2 H2O content, recorded as experiment 2 H2O content, and the body fluid loss rate was calculated by the following formula:

[0071] Body fluid loss rate = (experiment 2 H2O content - base 2 H2O content) / (test 2 H2O content - base 2 H2O content) × 100%.

[0072] Figure 2 The results of the body fluid loss test of the application examples 1-3 and the comparative examples 1-2 are shown in the figure, and the body fluid loss rates of the pure water group, the application examples 1-3 and the comparative examples 1-2 are 1.36%, 0.59%, 0.61%, 0.63% and 0.93%, 0.82% respectively; it is illustrated that the use of the inulin peptides and the inulin soluble fiber can obviously reduce the body fluid loss of the rats.

[0073] 3. Exercise capacity test

[0074] The water-deficient model rats were randomly divided into 6 groups, 5 rats in each group, and the rats in the control group, the application examples 1-3 and the comparative examples 1-2 were respectively gavaged with 10mL of water supplement liquid every 1h, wherein the rats in the control group were gavaged with pure water, and the rats in the application examples 1-3 and the comparative examples 1-2 were gavaged with the corresponding prepared beverages, after 4 times of gavage, the rats were subjected to exhaustive swimming test, and the rats were placed in a self-made water tank with a water depth of 35cm at 25℃ to swim freely, and the time from the start of the swimming of the rats to the sinking of the rats for 10s without floating out of the water was recorded, and recorded as the exhaustive swimming time of the rats.

[0075] Figure 3 The results of the exercise capacity test of Examples 1-3 and Comparative Examples 1-2 of the present application are shown in the graph, and the exhaustive swimming times of the control group, Examples 1-3 and Comparative Examples 1-2 were 125 s, 169 s, 163 s, 165 s and 146 s, 152 s, respectively, indicating that the use of the muira puama peptide and muira puama soluble fiber significantly improved the exercise capacity of the rats.

[0076] Although embodiments of the present application have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and alterations can be made hereto without departing from the principles and spirit of the present application.

[0077] The above description of the present application and its embodiments is not restrictive, and the embodiments shown in the drawings are only one of the embodiments of the present application, and the actual application is not limited thereto. In summary, if a person of ordinary skill in the art is inspired by it, without departing from the purpose of the present application, without creative design, similar ways and embodiments to the technical solution should belong to the protection scope of the present application.

Claims

1. A low calorie electrolyte beverage, characterized by, It is prepared from the following components by weight: 1.3-1.5 parts of inca berry extract, 0.02-0.03 parts of vitamin C, 0.016-0.019 parts of vitamin B2, 0.15-0.25 parts of citric acid, 0.1-0.2 parts of sodium citrate, 0.02-0.03 parts of sodium chloride, 0.02-0.03 parts of potassium chloride, 0.01-0.02 parts of stevia sugar and 150-180 parts of deionized water; The preparation method of the inca berry extract specifically comprises the following steps: S1, dry the inca berry meal, powder it, pass it through a 60-mesh sieve, add it to a 1.3wt% sodium chloride solution, then add glucose, mix well, and cook under normal pressure for 20-30 min to obtain a fermentation substrate; the inca berry meal is added in an amount of 0.5-0.6 g / mL in the 1.3wt% sodium chloride solution; the glucose is added in an amount of 6-8 mg / mL; S2, transfer the fermentation substrate obtained in S1 to a sealed fermentation tank, inoculate it with East Is a yeast, ferment it at 28℃ for 10 h, inoculate it with Clostridium cosel, increase the temperature to 35℃, and shake it at 120 rpm for 30 h, and finally store it at 4℃ for 6 h to obtain a fermentation product; the East Is a yeast is inoculated in an amount of 2-3%; the biological source is China Industrial Microbial Culture Collection Center, and the strain number is CICC32637; the Clostridium cosel is inoculated in an amount of 2-4%; the biological source is China Industrial Microbial Culture Collection Center, and the strain number is CICC8022; S3, pulp the fermentation product obtained in S2 with a pulper, filter it through a 150-mesh sieve to obtain a filtrate and a residue, centrifuge the filtrate for 10 min, take the supernatant, and freeze-dry it to obtain inca berry peptides; S4, add the residue obtained in S3 to a 3wt% sulfuric acid solution, stir it in a water bath, centrifuge it for 20 min, and take the supernatant; the residue is added in an amount of 0.05-0.06 g / mL in the 3wt% sulfuric acid solution; the stirring is performed in a water bath at a temperature of 60-70℃ and a speed of 80-120 rpm for 2-3 h; S5, add 3-4 times the volume of anhydrous ethanol to the supernatant obtained in S4, centrifuge it for 6 min after 16 h to take the precipitate, wash it, freeze-dry it to obtain inca berry soluble fiber, add the inca berry peptides obtained in S3, mix well to obtain inca berry extract.

2. A method of preparing a low calorie electrolyte beverage according to claim 1, characterized in that, Specifically, the following steps are included: add sodium chloride and potassium chloride to deionized water, stir and dissolve, add vitamin C, vitamin B2, citric acid, sodium citrate, inca berry extract and stevia sugar, stir and dissolve, filter, sterilize, and obtain a low-calorie electrolyte beverage.

3. The method of claim 2, wherein: In S3, the centrifugal speed is 3600-4200 rpm.

4. The method of claim 3, wherein: In S4, the centrifugal speed is 3000-4000 rpm.

5. The method of claim 4, wherein: In S5, the centrifugal speed is 2500-3000 rpm.

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