Composition with skin soothing and repairing effects and application thereof

Abstract

The invention relates to a composition with skin soothing and repairing effects and application thereof. The composition with the skin soothing and repairing effects comprises panthenol, seawater and carboxymethyl chitosan. The panthenol, the seawater and the carboxymethyl chitosan are creatively combined into a brand-new composition, the panthenol and the carboxymethyl chitosan have a synergistic effect, and the three components of the seawater are matched to have a potential mutual promotion effect; mild cool feeling can be brought to the skin, skin heating and redness are reduced, release of inflammatory factors is inhibited, the barrier of cuticle is repaired, and the moisturizing and water locking capacity of the skin is enhanced.

Description

Technical Field

[0001] The invention belongs to the technical field of daily cosmetics, and particularly relates to a composition with skin soothing and repairing effects and an application thereof. Background Art

[0002] With the negative impact of improper skin care, social pressure and bad living habits, the proportion of people with sensitive skin is increasing. 40%-56% of Asian women have sensitive skin. Sensitive skin will have damaged skin barrier function, dryness and roughness, excessive sensory nerve sensitivity, tingling, burning, itching and other discomforts, and immune inflammatory reactions such as redness, swelling and inflammation of the skin. Based on this, consumers have begun to accept the concept of "damaged barrier" and have begun to pay attention to barrier care. "Barrier repair" has surpassed "anti-aging" to become the most discussed topic at present.

[0003] CN118680859A discloses a composition with barrier repair and soothing effects, which utilizes the synergistic effect of tuberose, peony and epiphyllum to increase the richness of active functional ingredients in the composition, so that the prepared composition exhibits excellent moisturizing, anti-oxidation, antibacterial and bactericidal effects on the skin. The mass ratio of tuberose, peony and epiphyllum is specified to be (1-4): (0.1-0.4): (1-4), so that the active ingredients of the composition exhibit the best synergistic effect and stability, promote each other's penetration into the skin, stimulate skin cells and tissues from deep layers, and cure skin allergy symptoms; it can be used in cosmetics.

[0004] CN118557501A discloses a skin barrier repair composition and its application, belonging to the field of cosmetic technology. The skin barrier composition includes the following components in parts by weight: 0.1-2 parts of European red pine bud extract, 0.5-5 parts of sea star branch tube algae extract, 0.1-2 parts of pseudoalteromonas fermentation product extract, and 1-10 parts of egg fruit extract. The disclosure uses European red pine bud extract, sea star branch tube algae extract, pseudoalteromonas fermentation product extract and egg fruit extract as active ingredients to form a skin barrier repair composition. The components synergize to improve the effect of the barrier repair composition from four aspects: physical barrier, microbial barrier, chemical barrier and immune barrier.

[0005] Regardless of the factors that cause damaged skin barrier, it is essentially a destruction to the skin. Although the above-mentioned products can improve the skin barrier, their long-term repair effect on the skin's stratum corneum and the protective function of the skin is not obvious. Therefore, there is an urgent need to provide a more effective skin barrier repair product to meet the needs of consumers. Summary of the invention

[0006] In view of the deficiencies of the prior art, the object of the present invention is to provide a composition with skin soothing and repairing effects and its application.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a composition having skin soothing and repairing effects, wherein the composition having skin soothing and repairing effects comprises panthenol, sea water and carboxymethyl deacetylated chitosan.

[0009] Preferably, the seawater is Canadian glacial seawater on the west coast of the Pacific Ocean.

[0010] The panthenol described in the present invention is provitamin B5. As a commonly used raw material for skin care products, it has the effects of moisturizing, reducing skin TEWL, improving skin roughness and soothing irritation, and promoting wound repair and healing. The carboxymethyl deacetylated chitosan is derived from marine biopolysaccharides and has good film-forming properties. After being applied to the skin surface, a layer of biological sugar film can be formed to maintain moisturizing effect on the stratum corneum and prevent skin dryness. Multi-pathway soothing has significant effects on inflammation, itching, tingling, and redness caused by inflammatory factors, TRPV1, and histamine; and it can promote the formation of skin stratum corneum cells, accelerate the repair of skin stratum corneum, and avoid multiple allergies; the seawater is derived from Canadian glacier seawater on the west coast of the Pacific Ocean. The unique composition of glaciers and seawater makes seawater contain more than 70 marine rare earth elements and mineral elements, which are presented in a natural form that is completely soluble in water; seawater has a mild cooling feeling, which is different from the strong cooling feeling of cooling agents such as mints, and it has a mild cooling and soothing effect on the skin, and smoothes the burning sensation of the skin.

[0011] The present invention creatively combines panthenol, seawater and carboxymethyl chitosan into a new composition, wherein panthenol and carboxymethyl chitosan have a synergistic effect, and the combination with seawater has a potential mutually reinforcing effect, which can bring a mild cooling feeling to the skin, reduce fever and redness of the skin, inhibit the release of inflammatory factors, repair the barrier of the stratum corneum and enhance the skin's ability to moisturize and lock in water.

[0012] Preferably, the mass ratio of panthenol, seawater and carboxymethyl chitosan is (0.1-3):(0.1-3):(0.05-1).

[0013] The first "0.1-3" can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8 or 2.9, etc.;

[0014] The second "0.1-3" can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8 or 2.9, etc.;

[0015] “0.05-1” can be 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 or 0.9, etc.

[0016] Preferably, the mass ratio of panthenol, seawater and carboxymethyl chitosan is (0.5-2):(0.5-2):(0.05-0.5).

[0017] When the three components in the composition with skin soothing and repairing effects in the present invention are combined in the above-mentioned specific weight ratio, the soothing and repairing effects in terms of bringing a mild cooling sensation to the skin, reducing skin fever and redness, inhibiting the release of inflammatory factors, repairing the barrier of the stratum corneum and enhancing the skin's ability to moisturize and lock in water can be maximized.

[0018] Specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0019] In a second aspect, the present invention provides a use of the composition with skin soothing and repairing effects as described in the first aspect in the preparation of cosmetics.

[0020] Preferably, the cosmetics include essence, lotion, cream or mask.

[0021] Preferably, the mass percentage of the composition with skin soothing and repairing effects in the cosmetics is 0.1-10%, for example, it can be 0.3%, 0.5%, 0.8%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8% or 9%, etc.

[0022] Specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0023] In a third aspect, the present invention provides an essence with skin soothing and repairing effects, the components of which include the composition with skin soothing and repairing effects as described in the first aspect, a moisturizer, a thickener, an emollient, an emulsifier and water.

[0024] Preferably, the components of the essence with skin soothing and repairing effects include, by weight, 0.1-10 parts of the composition with skin soothing and repairing effects as described in the first aspect, 10-20 parts of moisturizer, 0.1-2 parts of thickener, 5-15 parts of emollient, 0.05-3 parts of emulsifier and 50-90 parts of water.

[0025] The amount of the composition with skin soothing and repairing effects added to the essence of the present invention can be 0.3 parts, 0.5 parts, 0.8 parts, 1 parts, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

[0026] The amount of the moisturizer added can be 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts or 19 parts, etc.;

[0027] The amount of the emollient added can be 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts or 14 parts, etc.;

[0028] The amount of the emulsifier added can be 0.05 parts, 0.1 parts, 0.3 parts, 0.5 parts, 0.8 parts, 1 part, 2 parts or 3 parts, etc.

[0029] The amount of water added can be 55 parts, 60 parts, 65 parts, 70 parts, 75 parts, 80 parts or 85 parts, etc.

[0030] Preferably, the moisturizing agent includes any one of glycerin, butylene glycol, 1,2-pentanediol, erythritol, betaine, sodium hyaluronate, dipropylene glycol or trehalose, or a combination of at least two thereof.

[0031] Preferably, the emollient includes any one or a combination of at least two of PPG-24-glyceryl polyether-24, PEG / PPG / polybutylene glycol-8 / 5 / 3 glycerol, squalane, camellia seed oil or C15-19 alkane.

[0032] Preferably, the emulsifier includes any one of hydrogenated lecithin, tri(laureth-4) phosphate, PEG-20 methyl glucose sesquistearate, glyceryl stearate citrate, PEG-10 soya bean sterol or sucrose stearate, or a combination of at least two thereof.

[0033] Preferably, the thickener includes any one of carbomer, sodium polyacrylate, ammonium acryloyldimethyltaurate / VP copolymer, polyacrylate crosspolymer-6, xanthan gum, carboxymethyl cellulose or hydroxyethyl cellulose, or a combination of at least two thereof.

[0034] Preferably, the essence with skin soothing and repairing effects also includes antioxidants;

[0035] Preferably, the essence with skin soothing and repairing effects also includes 1-5 parts of antioxidants by weight, for example, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts or 4.5 parts.

[0036] Preferably, the antioxidant comprises any one or a combination of at least two of tocopherol acetate, ascorbyl glucoside, ascorbic acid, ascorbyl palmitate, p-hydroxyacetophenone, chamomile extract, vaccinium angustifolium fruit extract or grape seed extract.

[0037] Preferably, the antioxidant is a combination of chamomile extract, vaccinium angustifolium fruit extract and grape seed extract.

[0038] The antioxidant of the present invention is a combination of chamomile extract, vaccinium angustifolium fruit extract and grape seed extract. The three are synergistically matched to enable the essence to have excellent effects of resisting excessive free radicals and improving the skin's own antioxidant capacity, thereby maintaining the skin in a stable and healthy state.

[0039] Preferably, the mass ratio of the chamomile extract, the vaccinium angustifolia fruit extract and the grape seed extract is (1-3):(1-5):(0.5-2).

[0040] Wherein "1-3" can be 1.1, 1.3, 1.5, 1.7, 2, 2.1, 2.3, 2.5, 2.7 or 2.9, etc.;

[0041] “1-5” can be 1.2, 1.5, 1.8, 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.24.5 or 4.8, etc.;

[0042] “0.5-2” can be 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.

[0043] Preferably, the essence with skin soothing and repairing effects also includes a skin conditioner.

[0044] Preferably, the essence with skin soothing and repairing effects also includes 0.1-5 parts of skin conditioners in parts by weight, for example, 0.2 parts, 0.5 parts, 0.7 parts, 0.9 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts or 4.5 parts, etc.

[0045] Preferably, the skin conditioning agent includes any one of dipotassium glycyrrhizinate, allantoin, niacinamide, ceramide NP, ceramide NS / NG, ceramide AP and vitamin E, or a combination of at least two thereof.

[0046] Preferably, the skin conditioning agent is a combination of dipotassium glycyrrhizinate, allantoin and ceramide NP.

[0047] As a preferred technical solution of the present invention, the dipotassium glycyrrhizinate, allantoin and ceramide NP cooperate synergistically to further enhance the ability of the essence to relieve skin inflammatory response, thereby enabling the essence to more significantly repair the barrier function of the skin.

[0048] Preferably, the mass ratio of dipotassium glycyrrhizinate, allantoin and ceramide NP is (0.1-1):(0.2-2):(0.1-1).

[0049] The first "0.1-1" can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 or 0.9, etc.;

[0050] “0.2-2” can be 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.;

[0051] The second "0.1-1" can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 or 0.9, etc.

[0052] Preferably, the essence with skin soothing and repairing effects further comprises any one or a combination of at least two of a preservative, a fragrance or a pH regulator.

[0053] Specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0054] In a fourth aspect, the present invention provides a method for preparing the essence according to the third aspect, the method comprising:

[0055] (1) mixing a moisturizer, an emollient, an emulsifier and a thickener in deionized water to obtain a mixed solution;

[0056] (2) adding the composition having skin soothing and repairing effects into the mixed solution and mixing them homogeneously to obtain the essence.

[0057] Preferably, the mixing temperature in step (1) is 60-80°C, for example, it can be 61°C, 63°C, 65°C, 67°C, 69°C, 70°C, 73°C, 75°C, 77°C or 79°C.

[0058] Preferably, the mixing temperature in step (2) is 30-45°C, for example, 31°C, 33°C, 35°C, 37°C, 40°C, 42°C or 44°C.

[0059] Preferably, the mixing in step (2) further comprises adding any one or a combination of at least two of an antioxidant, a skin conditioner, a preservative, a fragrance or a pH adjuster.

[0060] Specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0061] Compared with the prior art, the present invention has the following beneficial effects:

[0062] (1) The present invention creatively combines panthenol, seawater and carboxymethyl chitosan into a new composition, wherein panthenol and carboxymethyl chitosan have a synergistic effect, and the combination with seawater has a potential mutual promotion effect, which can bring a mild cooling feeling to the skin, reduce skin fever and redness, inhibit the release of inflammatory factors, repair the barrier of the stratum corneum and enhance the skin's ability to moisturize and lock in moisture.

[0063] (2) The essence provided by the present invention has a significant soothing and repairing effect by adding a composition with skin soothing and repairing effects. After use, consumers can keep their skin in a long-term, stable and healthy state.

[0064] (3) The preparation method of the essence provided by the present invention has simple operation steps, low cost, and is easy to industrialize and mass produce. DETAILED DESCRIPTION

[0065] In order to further illustrate the technical means and effects of the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the embodiments. Some of the materials and brand information involved in the following embodiments and comparative examples are as follows:

[0066]

[0067] The rest of the raw materials can be used as long as they are purchased from regular dealers.

[0068] Example 1

[0069] This embodiment provides a composition with skin soothing and repairing effects. The composition with skin soothing and repairing effects has a formula of 20 parts of panthenol, 20 parts of sea water and 5 parts of carboxymethyl deacetylated chitosan.

[0070] Example 2

[0071] This embodiment provides a composition with skin soothing and repairing effects. The composition with skin soothing and repairing effects has a formula of 17 parts of panthenol, 16 parts of seawater and 3 parts of carboxymethyl deacetylated chitosan.

[0072] Example 3

[0073] This embodiment provides a composition with skin soothing and repairing effects. The composition with skin soothing and repairing effects has a formula of 14 parts of panthenol, 12 parts of seawater and 1 part of carboxymethyl deacetylated chitosan.

[0074] Example 4

[0075] This embodiment provides a composition with skin soothing and repairing effects. The composition with skin soothing and repairing effects has a formula of 5 parts of panthenol, 20 parts of seawater and 20 parts of carboxymethyl deacetylated chitosan.

[0076] Example 5

[0077] This embodiment provides a composition with skin soothing and repairing effects. The composition with skin soothing and repairing effects has a formula of 24.9 parts of panthenol, 20 parts of seawater and 0.1 parts of carboxymethyl deacetylated chitosan.

[0078] Comparative Example 1

[0079] This comparative example provides a composition with skin soothing and repairing effects. The composition with skin soothing and repairing effects is different from Example 1 only in that panthenol is not included in the components, and its reduced amount is supplemented by carboxymethyl deacetylated chitosan, and the amounts of other components remain unchanged.

[0080] Comparative Example 2

[0081] This comparative example provides a composition with skin soothing and repairing effects. The composition with skin soothing and repairing effects is different from Example 1 only in that carboxymethyl deacetylated chitosan is not included in the components, and its reduced amount is supplemented by panthenol, and the amounts of other components remain unchanged.

[0082] Comparative Example 3

[0083] This comparative example provides a composition with skin soothing and repairing effects. The difference between the composition with skin soothing and repairing effects and Example 1 is that an equal amount of seawater is replaced by ordinary water, and the amounts of panthenol and carboxymethyl deacetylated chitosan are kept unchanged.

[0084] Application Examples 1-3

[0085] This application example provides three kinds of essences with skin soothing and repairing effects, and their formulas (contents are in parts) are as follows:

[0086]

[0087] Its preparation method is:

[0088] (1) heating deionized water to 80° C., then adding a moisturizer, a thickener, an emollient and an emulsifier, mixing them uniformly, then adding a neutralizer, and stirring them uniformly to obtain a mixed solution;

[0089] (2) The mixed solution is cooled to 40° C., and then the composition with skin soothing and repairing effects, the antioxidant and the skin conditioner are added to the mixed solution and mixed homogeneously to obtain the essence.

[0090] Application Example 4-5

[0091] This application example provides two essences, the formula of which is different from that of application example 1 only in that the composition of example 1 is replaced with the composition of examples 4-5 in equal amounts, and the other ingredients and contents remain unchanged. The preparation method is similar to that of application example 1.

[0092] Application Example 6

[0093] This application example provides an essence, the formula of which differs from that of application example 1 only in that it does not include an antioxidant, the reduced amount of which is supplemented by deionized water, and the other ingredients and contents remain unchanged. The preparation method is referred to application example 1.

[0094] Application Example 7

[0095] This application example provides an essence, the formula of which differs from that of application example 1 only in that the antioxidant does not include chamomile extract, and the reduced amount thereof is distributed to vaccinium angustifolium fruit extract and grape seed extract in proportion, while the other ingredients and contents remain unchanged. The preparation method is the same as that of application example 1.

[0096] Application Example 8

[0097] This application example provides an essence, the formula of which is different from that of application example 1 only in that the antioxidant does not include angustifolia vaccinium fruit extract, and the reduced amount thereof is distributed to chamomile extract and grape seed extract in proportion, while the other ingredients and contents remain unchanged. The preparation method is referred to application example 1.

[0098] Application Example 9

[0099] This application example provides an essence, the formula of which differs from that of application example 1 only in that grape seed extract is not included in the antioxidant, and its reduced amount is distributed to chamomile extract and vaccinium angustifolium fruit extract according to the proportion of parts, and other ingredients and contents remain unchanged. The preparation method is referred to application example 1.

[0100] Application Example 10

[0101] This application example provides an essence, the formula of which differs from that of application example 1 only in that it does not include a skin conditioning agent, the reduced amount of which is supplemented by deionized water, and the other ingredients and contents remain unchanged. The preparation method is referred to application example 1.

[0102] Application Example 11

[0103] This application example provides an essence, the formula of which differs from that of application example 1 only in that the skin conditioner does not include dipotassium glycyrrhizinate, and the reduced amount thereof is distributed to allantoin and ceramide NP according to the proportion of parts, while the other ingredients and contents remain unchanged. The preparation method is referred to application example 1.

[0104] Application Example 12

[0105] This application example provides an essence, the formula of which differs from that of application example 1 only in that allantoin is not included in the skin conditioning agent, and its reduced amount is distributed to dipotassium glycyrrhizinate and ceramide NP according to the proportion of parts, while other ingredients and contents remain unchanged. The preparation method is referred to application example 1.

[0106] Application Example 13

[0107] This application example provides an essence, the formula of which differs from that of application example 1 only in that ceramide NP is not included in the skin conditioning agent, and its reduced amount is distributed to dipotassium glycyrrhizinate and allantoin according to the proportion of parts, and other ingredients and contents remain unchanged. The preparation method is referred to application example 1.

[0108] Comparative Application Examples 1-3

[0109] This comparative application example provides three kinds of essences, the formulas of which differ from those of application example 1 only in that the composition of example 1 is replaced with the compositions of comparative examples 1-3 in equal amounts, and the other ingredients and contents remain unchanged. The preparation method is the same as that of application example 1.

[0110] Comparative Application Example 4

[0111] This comparative application example provides an essence, the formula of which differs from that of application example 1 only in that the composition of embodiment 1 is not included, the reduced amount of which is supplemented by water, and the other components and contents remain unchanged. The preparation method is similar to that of application example 1.

[0112] Test Example 1

[0113] Take 1g of the product of Application Example 1-13 and Comparative Application Example 1-4, add 2mL of anhydrous ethanol, and after complete dissolution, add 2mL of 60μmol / L DPPH solution and mix well, let it stand for 30min, adjust to zero with anhydrous ethanol, and measure the absorbance at 517nm as Ai; use the same method to take 2mL of anhydrous ethanol solvent + 2mL of DPPH solution and mix well to measure the absorbance extreme as Ac; take 1g of the composition matrix and dissolve it, add 2mL of anhydrous ethanol, and then add 2mL of anhydrous ethanol solvent and mix well to measure the absorbance as Aj.

[0114] The free radical scavenging rate was calculated according to the following formula.

[0115] Clearance (%) = [1-(Ai-Aj) / Ac] × 100%

[0116] Among them, Aj is the contribution of the reaction sample itself to the absorbance; Ai is the absorbance value of the sample after the action of DPPH; Ac is the absorption of DPPH itself at the measurement wavelength.

[0117] The DPPH free radical scavenging rate reflects its antioxidant capacity to a certain extent. The higher the scavenging rate, the better the antioxidant degree and the better the anti-aging effect. Therefore, the anti-aging effect of the composition can be judged by studying its ability to scavenge DPPH free radicals. The results are shown in Table 1 (each group of samples was measured 5 times in parallel, and the data are presented as average values).

[0118] Table 1

[0119]

[0120]

[0121] It can be seen from the test data in Table 1 that, compared with the comparative application example 4, the DPPH free radical scavenging rate of the essence described in the application examples 1-3 of the present invention can reach more than 87%, which is significantly improved, indicating that the composition with skin soothing and repairing effects has very good antioxidant activity, can effectively remove skin free radicals, and reduce oxidative stress-related inflammatory reactions; the DPPH free radical scavenging rate in application examples 6 and 10 has decreased, indicating that adding skin conditioners and antioxidants to the essence can significantly improve the antioxidant properties of the essence, and from application examples 7-9 and 11-13, it can be seen that the component selection of antioxidants and skin conditioners also affects the antioxidant properties. It can be seen from comparative application examples 1-2 that panthenol and carboxymethyl deacetylated chitosan in the composition with skin soothing and repairing effects of the present invention have a synergistic effect, and the lack of one of the two will reduce the antioxidant capacity of the essence to a certain extent, and the use of seawater can make the antioxidant effect of the composition more significant.

[0122] Test Example 2

[0123] Experiment to evaluate the inhibitory effect on inflammatory factors

[0124] Test sample: the essence provided in Application Examples 1-3, 10-13 and Comparative Application Examples 1-4;

[0125] Test principle: NO is an important pro-inflammatory mediator that mediates the occurrence and development of a variety of inflammatory reactions. By reducing its content, the inflammatory response can be inhibited, thereby achieving the effect of anti-inflammatory and allergy relief;

[0126] Test method: Take RAW264.7 cells in the logarithmic growth phase and plate them in a 12-well plate. After culturing at 37°C for 7 hours, discard the culture medium, add the samples provided in Application Examples 1-3, 10-13 and Comparative Application Examples 1-4, and pretreat for 1 hour. Then add LPS with a final concentration of 100 ng / mL to induce for 17 hours, collect the cell culture supernatant of each group, and detect the NO content. Among them, the blank group is without adding samples and LPS, the model group is without adding samples and only LPS induction, and the positive control group is 40μM dexamethasone. The specific test results are shown in Table 2 below:

[0127] Table 2

[0128]

[0129]

[0130] It can be seen from the data in Table 2 that, compared with Comparative Application Example 4, the relative content of NO in the essences described in Application Examples 1-3 of the present invention is below 120%, indicating that adding the composition with skin soothing and repairing effects of the present invention to the essences can significantly enhance the anti-inflammatory effect of the product.

[0131] It can be seen from Examples 10-13 that adding skin conditioners to the essence can improve the anti-inflammatory properties of the product, and the dipotassium glycyrrhizinate, allantoin and ceramide NP specifically selected in the present invention have a synergistic effect in terms of anti-inflammatory effect.

[0132] It can be seen from comparative application examples 1-3 that the combination of panthenol, seawater and carboxymethyl chitosan can significantly reduce inflammatory factors and inhibit the occurrence of inflammation.

[0133] Test Example 3: Skin barrier strengthening experiment

[0134] 102 volunteers aged 20 to 45 were randomly selected as research subjects, including 51 males and 51 females. The tested parts had no skin problems in the past month and no history of systemic diseases. The volunteers were randomly divided into 17 groups, 6 in each group, half males and half females. Before the test, the volunteers were required to wipe their arms with clean water and sit quietly for 30 minutes in a constant temperature and humidity environment (temperature: 25±1°C, relative humidity: 40±5%) to keep relaxed. A 4cm×4cm test area was marked on the inner side of the volunteer's arm 5cm away from the base of the palm. Multiple areas can be marked on the same arm at the same time (1cm apart). The products to be tested were randomly applied to the marked areas. The products (the essence prepared in Application Example 1-13 and Comparative Application Example 1-4) were applied to the marked areas of the volunteers for four consecutive weeks. The amount used was 2.0±0.1μL / cm 2During the test period, no drugs or cosmetics unrelated to the experiment were allowed to be applied. The transepidermal water loss tester (Tewameter TM300) was used to test and record the transepidermal water loss (TEWL0, TEWL1, TEWL2) before application, in the first week of application, and in the second week of application. The test results are shown in Table 3 (the results are the average of the results of 5 subjects).

[0135] Table 3

[0136] sample TEWL0 TEWL1 TEWL2 Application Example 1 13.28 7.89 5.89 Application Example 2 13.43 8.21 6.13 Application Example 3 13.35 8.03 6.07 Application Example 4 14.75 12.70 10.75 Application Example 5 14.61 11.93 9.98 Application Example 6 13.95 8.98 6.20 Application Example 7 13.72 8.97 6.91 Application Example 8 14.00 8.53 6.84 Application Example 9 13.89 8.84 6.99 Application Example 10 15.24 12.20 10.05 Application Example 11 14.83 11.98 9.82 Application Example 12 13.51 10.01 8.77 Application Example 13 13.33 10.73 8.90 Comparative application example 1 14.01 12.32 11.27 Comparative Application Example 2 13.64 10.26 9.01 Comparative Application Example 3 14.76 12.45 10.38 Comparative Application Example 4 15.04 14.68 13.55

[0137] As can be seen from Table 3, the essence of the present invention has excellent barrier repair ability, thereby enabling the skin to have excellent water-locking ability.

[0138] It can be seen from comparative application examples 1-3 that the combination of panthenol, seawater and carboxymethyl chitosan can significantly improve the skin barrier repair effect of the essence.

[0139] Test Example 4: Relief Test

[0140] The essence prepared by using Example 1-13 and Comparative Application Example 1-4 was tested for soothing effect:

[0141] The volunteers were the same as those in Experimental Example 3, and the grouping was the same. The subjects were not allowed to wear makeup or use skin care products on the morning of the test day. After cleaning the facial skin with clean water, they waited for 30 minutes in a constant temperature and humidity room. Then, 10% lactic acid was applied to the nasolabial groove on either side of the volunteers at room temperature. After 5 minutes of application, the essences of Application Examples 1-13 and Comparative Application Examples 1-4 were used respectively. After 30 minutes of use, the volunteers were asked to evaluate and score their feelings of use. A 5-point system was used: 4.5 points and above, indicating very good, very satisfied; 4-4.5 points were good; 3-4 points were moderately acceptable; and 3 points or less were not good, unacceptable. The scores of each group were the average of 6 people, and the results are shown in Table 4.

[0142] Table 4

[0143]

[0144]

[0145] As can be seen from Table 4, the essences prepared in Application Examples 1-3 can quickly regulate and relieve skin discomfort symptoms, have excellent immediate soothing effects, and volunteers have a good experience after use, indicating that the composition provided by the present invention has a rapid soothing effect. By comparing the products of Application Examples 1-3, it can be seen that the combination of panthenol, seawater and carboxymethyl deacetylated chitosan in the present invention has a more significant effect in soothing.

[0146] Test Example 5: Red blood cell hemolysis test

[0147] The red blood cell haemolysis test (RBC) is one of the alternative methods to the rabbit eye irritation test. The basic principle is to evaluate the damage of chemicals to eye tissue cells by measuring the amount of cell hemoglobin dissolution and the degree of denaturation. The RBC test can be used to evaluate the eye irritation of cosmetics.

[0148] The serum prepared in Example 1-13 was subjected to a red blood cell hemolysis test. The test method and judgment criteria of the RBC test of the European Center for Alternative Method Validation were adopted. The specific judgment criteria are shown in Table 5, where HD 50 is the sample concentration when 50% of red blood cells are hemolyzed, DI is the protein denaturation index, and L / D is HD 50 Ratio to DI.

[0149] Table 5

[0150] L / D Grading L / D>100 Non-irritating 10﹤L / D≦100 Slightly irritating 1﹤L / D≦10 Mild irritant 0.1﹤L / D≦1 Moderate irritation L / D≦0.1 Severe irritation

[0151] The irritation results of the tested samples are shown in Table 6.

[0152] Table 6

[0153]

[0154]

[0155] From the sample irritation results in Table 6, it can be seen that the irritation classification of the essence prepared by the present invention is non-irritating, which shows that the ingredients used in the essence of the present invention are mild and non-irritating.

[0156] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate a composition with skin soothing and repairing effects and its application, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary ingredients, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0157] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0158] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A composition having skin soothing and repairing effects, characterized in that: The composition with skin soothing and repairing effects comprises panthenol, sea water and carboxymethyl chitosan.

2. The composition having skin soothing and repairing effects as claimed in claim 1, characterized in that: The mass ratio of panthenol, seawater and carboxymethyl chitosan is (0.1-3):(0.1-3):(0.05-1); Preferably, the mass ratio of panthenol, seawater and carboxymethyl chitosan is (0.5-2):(0.5-2):(0.05-0.5).

3. Use of the composition with skin soothing and repairing effects as claimed in claim 1 or 2 in the preparation of cosmetics.

4. The use according to claim 3, characterized in that The cosmetics include essence, lotion, cream or mask; Preferably, the composition having the effect of soothing and repairing the skin has a mass percentage of 0.1-10% in the cosmetic.

5. An essence with skin soothing and repairing effects, characterized in that: The components of the essence with skin soothing and repairing effects include the composition with skin soothing and repairing effects as claimed in claim 1 or 2, a moisturizer, a thickener, an emollient, an emulsifier and water.

6. The essence with skin soothing and repairing effect as claimed in claim 5, characterized in that: The components of the essence with skin soothing and repairing effects include, by weight, 0.1-10 parts of the composition with skin soothing and repairing effects as claimed in claim 1 or 2, 10-20 parts of moisturizer, 0.1-2 parts of thickener, 5-15 parts of emollient, 0.05-3 parts of emulsifier and 50-90 parts of water.

7. The essence with skin soothing and repairing effect according to claim 5 or 6, characterized in that: The moisturizing agent includes any one of glycerin, butylene glycol, 1,2-pentanediol, erythritol, squalane, betaine, sodium hyaluronate, dipropylene glycol or trehalose, or a combination of at least two thereof; Preferably, the emollient comprises any one or a combination of at least two of PPG-24-glycerol polyether-24, PEG / PPG / polybutylene glycol-8 / 5 / 3 glycerol, squalane, camellia seed oil or C15-19 alkane; Preferably, the emulsifier includes any one or a combination of at least two of hydrogenated lecithin, tri(laureth-4) phosphate, PEG-20 methyl glucose sesquistearate, glyceryl stearate citrate, PEG-10 soya bean sterol or sucrose stearate; Preferably, the thickener includes any one of carbomer, sodium polyacrylate, ammonium acryloyldimethyltaurate / VP copolymer, polyacrylate crosspolymer-6, xanthan gum, carboxymethyl cellulose or hydroxyethyl cellulose, or a combination of at least two thereof.

8. The essence with skin soothing and repairing effect according to any one of claims 5 to 7, characterized in that: The essence with skin soothing and repairing effects also includes antioxidants in its components; Preferably, the essence with skin soothing and repairing effects also includes 1-5 parts of antioxidants by weight; Preferably, the antioxidant comprises any one or a combination of at least two of tocopherol acetate, ascorbyl glucoside, ascorbic acid, ascorbyl palmitate, p-hydroxyacetophenone, chamomile extract, vaccinium angustifolium fruit extract or grape seed extract; Preferably, the antioxidant is a combination of chamomile extract, vaccinium angustifolium fruit extract and grape seed extract.

9. The essence with skin soothing and repairing effect according to any one of claims 5 to 8, characterized in that: The essence with skin soothing and repairing effects also includes skin conditioning agents; Preferably, the essence with skin soothing and repairing effects further comprises 0.1-5 parts of skin conditioning agents by weight; Preferably, the skin conditioning agent comprises any one or a combination of at least two of dipotassium glycyrrhizinate, allantoin, niacinamide, ceramide NP, ceramide NS / NG, ceramide AP and vitamin E; Preferably, the skin conditioning agent is a combination of dipotassium glycyrrhizinate, allantoin and ceramide NP; Preferably, the essence with skin soothing and repairing effects further comprises any one or a combination of at least two of a preservative, a fragrance or a pH regulator.

10. A method for preparing the essence according to any one of claims 5 to 9, characterized in that: The method comprises: (1) mixing a moisturizer, an emollient, an emulsifier and a thickener in deionized water to obtain a mixed solution; (2) adding the composition having skin soothing and repairing effects into the mixed solution and mixing until homogeneous to obtain the essence; Preferably, the mixing temperature in step (1) is 60-80°C; Preferably, the mixing temperature in step (2) is 30-45°C; Preferably, the mixing in step (2) further comprises adding any one or a combination of at least two of an antioxidant, a skin conditioner, a preservative, a fragrance or a pH adjuster.

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