A composition for repairing sensitive skin, its preparation method and application in cosmetics

Abstract

The present invention belongs to the technical field of cosmetics, and specifically relates to a composition for repairing sensitive skin, a preparation method thereof, and an application in cosmetics. The present invention uses raw materials such as collagen, soluble collagen, hydrolyzed collagen, asiaticoside, albizia flower extract, ledebouriella root extract, filtrate of yeast ferment lysate, filtrate of bifidobacterium ferment lysate, bacillus ferment product, carnosine, polygonum cuspidatum root extract, and ganoderma atrum extract as active ingredients, which cooperate with each other and act together to endow the composition with excellent safety, anti-inflammatory repair, and moisturizing and skin-nourishing effects.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to a composition for repairing sensitive skin, a preparation method thereof, and an application in cosmetics. Background Art

[0002] The main reasons for sensitive skin include impaired or damaged skin barrier function and external stimuli. Sensitive skin is generally divided into two types: congenital and acquired. Congenital sensitive skin: mostly genetic, with naturally thinner skin, obvious red blood streaks, and is easily affected by changes in the external environment. Acquired sensitive skin: caused by some stimuli or improper skin care behaviors, resulting in the skin becoming sensitive and fragile, damaging the outermost sebum membrane of the skin, reducing skin resistance, thinning the cutin layer, and being unable to play a normal protective role.

[0003] Repairing sensitive skin refers to improving and repairing sensitive skin through a series of skin care measures to restore it to a healthy state. At present, there are a wide variety of cosmetics for repairing sensitive skin on the market, but their quality is uneven. Although they have a certain soothing and repairing effect, their moisturizing and other performances are insufficient. Therefore, providing a composition with high safety and excellent effects of repairing sensitive skin and moisturizing the skin is a technical problem that needs to be solved at present. Summary of the Invention

[0004] In view of the above technical problems, the present invention provides a composition for repairing sensitive skin, a preparation method thereof, and an application in cosmetics.

[0005] On the one hand, the present invention provides a composition for repairing sensitive skin, and the raw material composition is: collagen, soluble collagen, hydrolyzed collagen, asiaticoside, albizia flower extract, saposhnikovia root extract, filtrate of yeast ferment lysate, filtrate of bifidobacterium ferment lysate, bacillus ferment lysate, carnosine, polygonum cuspidatum root extract, and ganoderma atrum extract. The above raw materials interact with each other and cooperate together to endow the composition with excellent safety, effects of repairing sensitive skin and moisturizing the skin.

[0006] Preferably, the composition, by mass, comprises the following raw materials: 0.001 - 0.2 parts of collagen, 0.001 - 0.1 parts of soluble collagen, 0.001 - 0.2 parts of hydrolyzed collagen, 0.05 - 1 part of asiaticoside, 0.005 - 0.04 parts of albizia flower extract, 0.05 - 1 part of saposhnikovia root extract, 0.05 - 0.8 parts of filtrate of yeast ferment lysate, 0.01 - 1 part of filtrate of bifidobacterium ferment lysate, 0.01 - 0.3 parts of bacillus ferment lysate, 0.001 - 0.1 parts of carnosine, 0.01 - 0.3 parts of polygonum cuspidatum root extract, and 0.05 - 1 part of ganoderma atrum extract.

[0007] Preferably, the extraction methods of Albizia julibrissin Durazz. flower extract and Polygonum cuspidatum Sieb. et Zucc. root extract can be obtained according to conventional extraction methods. The extraction methods include but are not limited to: crushing Albizia julibrissin Durazz. flowers or Polygonum cuspidatum Sieb. et Zucc. roots, then mixing them with water at a mass ratio of 1:10 - 50, heating and stirring for extraction, filtering, and drying to obtain the extract.

[0008] Preferably, the extraction method of Saposhnikovia divaricata (Turcz.) Schischk. root extract includes: crushing Saposhnikovia divaricata (Turcz.) Schischk. roots, then mixing them with 20 - 35wt% ethanol aqueous solution at a mass ratio of 1:10 - 50, heating and stirring at 45 - 60°C for 5 - 12 hours, filtering, and drying the filtrate to obtain Saposhnikovia divaricata (Turcz.) Schischk. root extract.

[0009] Preferably, the extraction method of Ganoderma atrum extract includes: crushing Ganoderma atrum, obtaining crushed materials; adding 50 - 75wt% ethanol aqueous solution at 10 - 30 times the mass of the crushed materials, heating and stirring at 50 - 60°C for 2 - 5 hours, then filtering to obtain a filtrate; concentrating the filtrate under reduced pressure at 50 - 60°C to a mass of 0.8 - 1.5 times that of the crushed materials to obtain a concentrate; mixing the concentrate with an extractant composed of water and an organic solvent at a mass ratio of 1:2 - 4, the organic solvent being composed of ethyl acetate and chloroform at a mass ratio of 2 - 3.5:1, then stirring and extracting at a temperature of 30 - 40°C for 2 - 4 hours, then standing for layer separation, and concentrating the separated oil phase under reduced pressure until ethyl acetate and chloroform are undetectable, stopping the reduced pressure distillation to obtain Ganoderma atrum extract.

[0010] Preferably, the mass ratio of madecassoside and Ganoderma atrum extract is 0.4 - 0.8:1.

[0011] Preferably, the mass ratio of Saposhnikovia divaricata (Turcz.) Schischk. root extract and yeast fermentation lysate filtrate is 1:1 - 1.5.

[0012] Preferably, the composition described above, by mass, includes the following raw materials: 0.005 - 0.15 parts of collagen, 0.005 - 0.08 parts of soluble collagen, 0.005 - 0.1 parts of hydrolyzed collagen, 0.08 - 0.9 parts of madecassoside, 0.01 - 0.03 parts of Albizia julibrissin Durazz. flower extract, 0.08 - 0.9 parts of Saposhnikovia divaricata (Turcz.) Schischk. root extract, 0.08 - 0.75 parts of yeast fermentation lysate filtrate, 0.05 - 0.5 parts of bifidobacterium fermentation product filtrate, 0.05 - 0.25 parts of bacillus fermentation product, 0.005 - 0.08 parts of carnosine, 0.05 - 0.2 parts of Polygonum cuspidatum Sieb. et Zucc. root extract, and 0.08 - 0.9 parts of Ganoderma atrum extract.

[0013] Preferably, the composition described above, by mass, may further include 0.1 - 5 parts of butanediol.

[0014] Preferably, the composition described above, by mass, may further include 5 - 50 parts of water.

[0015] On the other hand, the present invention also provides a method for preparing the composition, which includes stirring and mixing the filtrate of yeast fermentation lysate, the filtrate of bifidobacterium fermentation product, the extract of ganoderma atrum, and the extract of saposhnikovia root, then adding the remaining raw materials and continuing to stir, and sterilizing to obtain the composition.

[0016] On the other hand, the present invention also provides the application of the composition in the preparation of cosmetics, and the dosage of the composition in cosmetics is 0.1 - 30 wt%.

[0017] Preferably, the dosage form of the cosmetics includes aqueous solution, emulsion, paste, cream, powder.

[0018] Preferably, the cosmetics include facial mask, paste, cream, emulsion, essence, foundation, eyeshadow, blush, shampoo, perfume, etc.

[0019] Advantages of the present invention:

[0020] The present invention uses raw materials such as collagen, soluble collagen, hydrolyzed collagen, asiaticoside, albizia flower extract, saposhnikovia root extract, filtrate of yeast fermentation lysate, filtrate of bifidobacterium fermentation product, bacillus fermentation product, carnosine, polygonum cuspidatum root extract, and ganoderma atrum extract as active ingredients, which cooperate with each other and act together to endow the composition with excellent performance.

[0021] The composition prepared by the present invention has no irritation to the skin, high safety, and excellent anti-inflammatory and skin repair effects.

[0022] The present invention uses the combination of ethyl acetate and chloroform as the organic solvent in the extractant. Compared with using only one of them or replacing with other organic solvents to extract ganoderma atrum, the prepared ganoderma atrum extract has better anti-inflammatory effects and can endow the composition with better anti-inflammatory and skin repair performance; when extracting in the present invention, the co-compounding of ethyl acetate and chloroform produces a mutually promoting extraction effect and improves the anti-inflammatory performance of the ganoderma atrum extract.

[0023] The present invention uses the combined action of asiaticoside and ganoderma atrum extract to synergistically enhance the anti-inflammatory performance of the composition. The mass ratio of asiaticoside to ganoderma atrum extract in the present invention is 0.4 - 0.8:1, and the obtained composition has better anti-inflammatory performance.

[0024] The composition prepared by the present invention can effectively improve the skin water content and has good moisturizing and skin nourishing effects.

[0025] The moisturizing effect of the saposhnikovia root extract prepared by the present invention using a high-concentration ethanol solution is relatively poor. The present invention uses a 20 - 35 wt% ethanol aqueous solution to extract the saposhnikovia root extract, which has better moisturizing performance.

[0026] The present invention uses a combination of a radix fangfeng extract and a yeast fermentation lysate filtrate to mutually promote the moisturizing and emollient properties of the composition. When the mass ratio of the radix fangfeng extract to the yeast fermentation lysate filtrate is 1:1-1.5, the obtained composition has better moisturizing and emollient properties.

[0027] The product of the invention has excellent effect, simple preparation method and good application prospect. DETAILED DESCRIPTION

[0028] The present invention will be described below in conjunction with specific embodiments, and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments are used to illustrate the present invention, rather than to limit the present invention.

[0029] The raw material information of the specific implementation mode of the present invention is as follows:

[0030] The extraction method of the Albizzia julibrissin flower extract is as follows: the dried Albizzia julibrissin flower is crushed, then mixed with water in a mass ratio of 1:35, heated to 60°C and stirred at 60 rpm for 5 hours, filtered, and freeze-dried at -40°C to a water content of 3.62wt% to obtain the Albizzia julibrissin flower extract.

[0031] Extraction method of Polygonum cuspidatum root extract: grind the dried Polygonum cuspidatum root, mix it with water in a mass ratio of 1:28, heat it to 62°C, stir it at 70 rpm for 4.8 hours, filter it, and dry it at -42°C to a water content of 3.02wt% to obtain the Polygonum cuspidatum root extract.

[0032] Other raw material information is shown in Table 1.

[0033] Table 1: Raw material information

[0034]

[0035] 1. Preparation of products

[0036] Composition 1:

[0037] According to mass parts, the raw materials are composed of:

[0038] 0.08 parts of collagen, 0.02 parts of soluble collagen, 0.01 parts of hydrolyzed collagen, 0.35 parts of madecassoside, 0.03 parts of Albizia Julibrissin flower extract, 0.5 parts of Saposhnikovia root extract, 0.6 parts of yeast fermentation lysate filtrate, 0.08 parts of Bifida fermentation product filtrate, 0.09 parts of Bacillus fermentation product, 0.02 parts of carnosine, 0.1 parts of Polygonum cuspidatum root extract, 0.5 parts of black ganoderma lucidum extract, 3 parts of 1,3-butylene glycol, and water makes up to 40 parts.

[0039] Extraction method of Saposhnikovia root extract: The dried Saposhnikovia root is crushed to a particle size below 20 mesh, then mixed with 25wt% ethanol aqueous solution at a mass ratio of 1:35, heated at 60°C and stirred at 70 rpm for 10 hours, filtered through a 0.2-micron pore size filter membrane, and the filtrate is freeze-dried at -42°C until the water content is 2.50wt% to obtain the Saposhnikovia root extract.

[0040] Extraction method of Ganoderma atrum extract: The dried Ganoderma atrum is crushed to a particle size below 20 mesh to obtain a crushed material; 25 times the mass of the crushed material of 65wt% ethanol aqueous solution is added, heated at 55°C and stirred at 70 rpm for 3.5 hours, and then filtered through a 0.2-micron pore size filter membrane to obtain a filtrate; the filtrate is concentrated under reduced pressure at 55°C and 0.005 MPa to a mass 1 time that of the crushed material to obtain a concentrate; the concentrate is mixed with an extractant composed of water and an organic solvent at a mass ratio of 1:3, the organic solvent is composed of ethyl acetate and chloroform at a mass ratio of 3:1, and then stirred and extracted at 70 rpm at 35°C for 3 hours, and then left to stand and separate for 2 hours. The oil phase obtained by separation is concentrated under reduced pressure at 40°C and 0.002 Mpa until ethyl acetate and chloroform are not detected, and the reduced pressure distillation is stopped to obtain the Ganoderma atrum extract.

[0041] The preparation method of the composition is as follows:

[0042] The filtrate of the yeast fermentation lysate, the filtrate of the bifidobacterium fermentation product, the Ganoderma atrum extract, and the Saposhnikovia root extract are stirred and mixed at 80 rpm for 0.3 hours, then the remaining raw materials are added and stirred at 80 rpm for 0.5 hours, and sterilized to obtain the composition.

[0043] Composition 2:

[0044] According to parts by mass, the raw material composition:

[0045] 0.06 parts of collagen, 0.01 part of soluble collagen, 0.04 part of hydrolyzed collagen, 0.29 part of asiaticoside, 0.029 part of Albizia julibrissin extract, 0.45 part of Saposhnikovia root extract, 0.65 part of yeast fermentation lysate filtrate, 0.07 part of bifidobacterium fermentation product filtrate, 0.11 part of Bacillus fermentation product, 0.012 part of carnosine, 0.09 part of Polygonum cuspidatum root extract, 0.56 part of Ganoderma atrum extract, 3 parts of 1,3-butanediol, and water is made up to 40 parts.

[0046] Extraction method of Saposhnikovia root extract: The dried Saposhnikovia root is crushed to a particle size below 20 mesh, then mixed with 20wt% ethanol aqueous solution at a mass ratio of 1:45, heated at 45°C and stirred at 80 rpm for 9.5 hours, filtered through a 0.2-micron pore size filter membrane, and the filtrate is freeze-dried at -42°C until the water content is 2.47wt% to obtain the Saposhnikovia root extract.

[0047] Extraction method of Ganoderma atrum extract: The dried Ganoderma atrum is crushed to a particle size below 20 mesh to obtain a crushed material; 25 times the mass of the crushed material of 75wt% ethanol aqueous solution is added, heated at 52 °C and stirred at 70 rpm for 4 hours, and then filtered through a filter membrane with a pore size of 0.2 microns to obtain a filtrate; the filtrate is concentrated under reduced pressure at 55 °C and 0.005 MPa to a mass 1 time that of the crushed material to obtain a concentrate; the concentrate is mixed with an extractant composed of water and an organic solvent in a mass ratio of 1:2, and the organic solvent is composed of ethyl acetate and chloroform in a mass ratio of 3.5:1, and then stirred and extracted at 38 °C and 70 rpm for 3.5 hours, and then left to stand and layer for 2.5 hours. The oil phase obtained by layering is concentrated under reduced pressure at 40 °C and 0.002 Mpa until ethyl acetate and chloroform are not detected, and the reduced pressure distillation is stopped to obtain Ganoderma atrum extract.

[0048] The preparation method of the composition is as follows:

[0049] The filtrate of yeast fermentation lysate, the filtrate of bifidobacterium fermentation product, Ganoderma atrum extract, and Ledebouriella root extract are stirred and mixed at 90 rpm for 0.25 hours, and then the remaining raw materials are added and stirred at 100 rpm for 0.4 hours, and then sterilized to obtain the composition.

[0050] Composition 3:

[0051] According to parts by mass, the raw material composition:

[0052] 0.07 parts of collagen, 0.015 parts of soluble collagen, 0.025 parts of hydrolyzed collagen, 0.37 parts of asiaticoside, 0.032 parts of Albizia julibrissin Durazz. flower extract, 0.55 parts of Ledebouriella root extract, 0.55 parts of yeast fermentation lysate filtrate, 0.09 parts of bifidobacterium fermentation product filtrate, 0.07 parts of Bacillus fermentation product, 0.03 parts of carnosine, 0.12 parts of Polygonum cuspidatum Sieb. et Zucc. root extract, 0.48 parts of Ganoderma atrum extract, 3 parts of 1,3-butanediol, and water is made up to 40 parts.

[0053] Extraction method of Ledebouriella root extract: The dried Ledebouriella root is crushed to a particle size below 20 mesh, and then mixed with 35wt% ethanol aqueous solution in a mass ratio of 1:20, heated at 45 °C and stirred at 80 rpm for 12 hours, filtered through a filter membrane with a pore size of 0.2 microns, and the filtrate is freeze-dried at -42 °C until the water content is 2.61wt% to obtain Ledebouriella root extract.

[0054] Extraction method of Ganoderma atrum extract: The dried Ganoderma atrum is crushed to a particle size below 20 mesh to obtain a crushed material; 20 times the mass of the crushed material of 50wt% ethanol aqueous solution is added, heated at 57°C and stirred at 70 rpm for 2.5 hours, and then filtered through a 0.2-micron pore size filter membrane to obtain a filtrate; the filtrate is concentrated under reduced pressure at 55°C and 0.005 MPa to a mass 0.9 times that of the crushed material to obtain a concentrate; the concentrate is mixed with an extractant composed of water and an organic solvent in a mass ratio of 1:4, and the organic solvent is composed of ethyl acetate and chloroform in a mass ratio of 2:1, and then stirred and extracted at 33°C and 70 rpm for 2.5 hours, and then allowed to stand and layer for 2.5 hours, and the oil phase obtained by layering is concentrated under reduced pressure at 40°C and 0.002 Mpa until ethyl acetate and chloroform are undetectable, and the reduced pressure distillation is stopped to obtain Ganoderma atrum extract.

[0055] The preparation method of the composition is as follows:

[0056] The filtrate of yeast fermentation lysate, the filtrate of bifidobacterium fermentum, Ganoderma atrum extract, and Saposhnikovia divaricata root extract are stirred and mixed at 75 rpm for 0.35 hours, and then the remaining raw materials are added and stirred at 95 rpm for 0.55 hours, and sterilized to obtain the composition.

[0057] Composition 4:

[0058] The difference compared with Composition 1 is that the extraction method of Saposhnikovia divaricata root extract has changed, and the concentration of the ethanol aqueous solution used in the extraction has increased. Specifically: The dried Saposhnikovia divaricata root is crushed to a particle size below 20 mesh, and then mixed with 65wt% ethanol aqueous solution in a mass ratio of 1:35, heated at 60°C and stirred at 70 rpm for 10 hours, filtered through a 0.2-micron pore size filter membrane, and the filtrate is freeze-dried at -42°C until the water content is 2.50wt% to obtain Saposhnikovia divaricata root extract. The others are the same as Composition 1.

[0059] Composition 5:

[0060] The difference compared with Composition 1 is that the extraction method of Ganoderma atrum extract has changed, and the organic solvent uses cyclohexane to replace ethyl acetate and chloroform in Composition 1. Specifically:

[0061] The dried Ganoderma atrum is crushed to a particle size below 20 mesh to obtain a crushed material; 25 times the mass of the crushed material of 65 wt% ethanol aqueous solution is added, heated at 55 °C and stirred at 70 rpm for 3.5 hours, and then filtered through a filter membrane with a pore size of 0.2 μm to obtain a filtrate; the filtrate is concentrated under reduced pressure at 55 °C and 0.005 MPa to a mass 1 time that of the crushed material to obtain a concentrate; the concentrate is mixed with an extractant composed of water and an organic solvent in a mass ratio of 1:3, the organic solvent is cyclohexane, and then stirred and extracted at 70 rpm at a temperature of 35 °C for 3 hours, and then allowed to stand and separate for 2 hours. The oil phase obtained by separation is concentrated under reduced pressure at 40 °C and 0.002 Mpa until cyclohexane is undetectable, and the reduced pressure distillation is stopped to obtain the Ganoderma atrum extract.

[0062] Others are the same as those of Composition 1.

[0063] Composition 6:

[0064] The difference compared with Composition 1 is that the extraction method of the Ganoderma atrum extract has changed, and only ethyl acetate is used as the organic solvent. Specifically:

[0065] The dried Ganoderma atrum is crushed to a particle size below 20 mesh to obtain a crushed material; 25 times the mass of the crushed material of 65 wt% ethanol aqueous solution is added, heated at 55 °C and stirred at 70 rpm for 3.5 hours, and then filtered through a filter membrane with a pore size of 0.2 μm to obtain a filtrate; the filtrate is concentrated under reduced pressure at 55 °C and 0.005 MPa to a mass 1 time that of the crushed material to obtain a concentrate; the concentrate is mixed with an extractant composed of water and an organic solvent in a mass ratio of 1:3, the organic solvent is ethyl acetate, and then stirred and extracted at 70 rpm at a temperature of 35 °C for 3 hours, and then allowed to stand and separate for 2 hours. The oil phase obtained by separation is concentrated under reduced pressure at 40 °C and 0.002 Mpa until ethyl acetate is undetectable, and the reduced pressure distillation is stopped to obtain the Ganoderma atrum extract.

[0066] Others are the same as those of Composition 1.

[0067] Composition 7:

[0068] The difference compared with Composition 1 is that the extraction method of the Ganoderma atrum extract has changed, and only chloroform is used as the organic solvent. Specifically:

[0069] Dry Ganoderma atrum is crushed to a particle size below 20 mesh to obtain a crushed material; 25 times the mass of the crushed material of 65 wt% ethanol aqueous solution is added, heated at 55 °C and stirred at 70 rpm for 3.5 hours, and then filtered through a 0.2-micron pore size filter membrane to obtain a filtrate; the filtrate is concentrated under reduced pressure at 55 °C and 0.005 MPa to a mass 1 time that of the crushed material to obtain a concentrate; the concentrate is mixed with an extractant composed of water and an organic solvent in a mass ratio of 1:3, the organic solvent is chloroform, and then stirred and extracted at 70 rpm at 35 °C for 3 hours, and then left to stand for 2 hours to separate layers. The oil phase obtained by layering is concentrated under reduced pressure at 40 °C and 0.002 Mpa until chloroform is undetectable, and the reduced pressure distillation is stopped to obtain Ganoderma atrum extract.

[0070] Others are the same as those of Composition 1.

[0071] Composition 8:

[0072] The difference compared with Composition 1 is that asiaticoside is not used in the composition formula, and the dosage of Ganoderma atrum extract is 0.85 parts. Specifically: 0.08 parts of collagen, 0.02 parts of soluble collagen, 0.01 parts of hydrolyzed collagen, 0.03 parts of Albizia julibrissin Durazz. flower extract, 0.5 parts of Saposhnikovia divaricata (Turcz.) Schischk. root extract, 0.6 parts of filtrate of yeast fermentation lysate, 0.08 parts of filtrate of Saccharomyces boulardii fermentation product, 0.09 parts of Bacillus fermentation product, 0.02 parts of carnosine, 0.1 parts of Polygonum cuspidatum Sieb. et Zucc. root extract, 0.85 parts of Ganoderma atrum extract, 3 parts of 1,3-butanediol, and water is added up to 40 parts.

[0073] Others are the same as those of Composition 1.

[0074] Composition 9:

[0075] The difference compared with Composition 1 is that Ganoderma atrum extract is not used in the composition formula, and the dosage of asiaticoside is 0.85 parts. Specifically: 0.08 parts of collagen, 0.02 parts of soluble collagen, 0.01 parts of hydrolyzed collagen, 0.85 parts of asiaticoside dosage, 0.03 parts of Albizia julibrissin Durazz. flower extract, 0.5 parts of Saposhnikovia divaricata (Turcz.) Schischk. root extract, 0.6 parts of filtrate of yeast fermentation lysate, 0.08 parts of filtrate of Saccharomyces boulardii fermentation product, 0.09 parts of Bacillus fermentation product, 0.02 parts of carnosine, 0.1 parts of Polygonum cuspidatum Sieb. et Zucc. root extract, 3 parts of 1,3-butanediol, and water is added up to 40 parts.

[0076] Others are the same as those of Composition 1.

[0077] Composition 10:

[0078] It is different from Composition 1 in that the costus root extract is not used in the composition formula, and the amount of the filtrate of yeast fermented lysate is 1.1 parts. Specifically: 0.08 parts of collagen, 0.02 parts of soluble collagen, 0.01 parts of hydrolyzed collagen, 0.35 parts of asiaticoside, 0.03 parts of albizia flower extract, 1.1 parts of the filtrate of yeast fermented lysate, 0.08 parts of bifida ferment lysate, 0.09 parts of bacillus ferment, 0.02 parts of carnosine, 0.1 parts of polygonum cuspidatum root extract, 0.5 parts of ganoderma atrum extract, 3 parts of 1,3-butanediol, and water is added to make up to 40 parts.

[0079] The others are the same as those of Composition 1.

[0080] Composition 11:

[0081] It is different from Composition 1 in that the filtrate of yeast fermented lysate is not used in the composition formula, and the amount of costus root extract is 1.1 parts. Specifically: 0.08 parts of collagen, 0.02 parts of soluble collagen, 0.01 parts of hydrolyzed collagen, 0.35 parts of asiaticoside, 0.03 parts of albizia flower extract, 1.1 parts of costus root extract, 0.08 parts of bifida ferment lysate, 0.09 parts of bacillus ferment, 0.02 parts of carnosine, 0.1 parts of polygonum cuspidatum root extract, 0.5 parts of ganoderma atrum extract, 3 parts of 1,3-butanediol, and water is added to make up to 40 parts.

[0082] The others are the same as those of Composition 1.

[0083] Composition 12:

[0084] It is different from Composition 1 in that the ratio of the amounts of asiaticoside and ganoderma atrum extract used in the composition formula changes. Specifically: 0.08 parts of collagen, 0.02 parts of soluble collagen, 0.01 parts of hydrolyzed collagen, 0.08 parts of asiaticoside, 0.03 parts of albizia flower extract, 0.5 parts of costus root extract, 0.6 parts of the filtrate of yeast fermented lysate, 0.08 parts of bifida ferment lysate, 0.09 parts of bacillus ferment, 0.02 parts of carnosine, 0.1 parts of polygonum cuspidatum root extract, 0.77 parts of ganoderma atrum extract, 3 parts of 1,3-butanediol, and water is added to make up to 40 parts. The mass ratio of asiaticoside to ganoderma atrum extract is 0.104:1

[0085] The others are the same as those of Composition 1.

[0086] Composition 13:

[0087] It is different from Composition 1 in that the dosage ratio of the radix saposhnikoviae extract and the filtrate of yeast fermented lysate in the composition formula has changed. Specifically: 0.08 parts of collagen, 0.02 parts of soluble collagen, 0.01 part of hydrolyzed collagen, 0.35 parts of asiaticoside, 0.03 parts of albizia flower extract, 0.1 part of radix saposhnikoviae extract, 1 part of filtrate of yeast fermented lysate, 0.08 parts of filtrate of bifidobacterium fermented product, 0.09 parts of bacillus fermented product, 0.02 parts of carnosine, 0.1 part of polygonum cuspidatum root extract, 0.5 part of ganoderma atrum extract, 3 parts of 1,3-butanediol, and water is added to make up 40 parts. The mass ratio of the radix saposhnikoviae extract to the filtrate of yeast fermented lysate is 1:10.

[0088] Others are the same as Composition 1.

[0089] II. Tests

[0090] 1. Skin patch test

[0091] Healthy volunteers aged 35 - 45 were selected and randomly divided into 13 groups with 15 people in each group. The skin on the front inner side of the right arm of the volunteers was cleaned with clear water and dried naturally in the natural wind as the test area; then a patch tester was selected, and in a closed patch test method, 0.025 g of the above composition was taken in the patch tester and applied to the test area of the subjects. After 24 h, the patch tester was removed, and after another 60 min, the skin appearance of each group of volunteers was evaluated according to the grade standard recorded in Table 2, as shown in Table 3.

[0092] Table 2: Grade standard

[0093]

[0094] Table 3: Results of the patch test

[0095]

[0096] According to the test results in Table 3, it can be seen that the composition prepared by the present invention has no irritation to the skin and has high safety.

[0097] 2. Anti-inflammatory and repair test

[0098] The anti-inflammatory and skin repair effect of the composition was tested by measuring the level of inhibition of inflammatory factor expression by the composition.

[0099] Experimental steps: HaCaT cells were cultured in DMEM medium at 37 °C and 5% CO2 environment, and the medium was changed every two days. When the cell confluence reached about 90%, it was applied to different groups for testing.

[0100] Group setting and drug administration setting:

[0101] Blank group: Only cultured in DMEM medium for 48 h.

[0102] Model group: Stimulate with LPS at a final concentration of 1 μg / mL for 24 h, and then replace it with DMEM medium and continue to culture for 24 h.

[0103] Composition group: Stimulate with LPS at a final concentration of 1 μg / mL for 24 h, and then replace it with DMEM medium containing 8 mg / mL of compositions 1-3, 5-9, 12 and continue to culture for 24 h.

[0104] Positive drug group: Stimulate with LPS at a final concentration of 1 μg / mL for 24 h, and then replace it with DMEM medium containing 9 μg / mL of dexamethasone and continue to culture for 24 h.

[0105] Take the supernatant of each group after culture, and use an ELISA kit to test the contents of inflammatory factors TNF-α and IL-1β in each group according to the method described in the kit instructions. Measure in parallel three times and take the average value; then record the reduction in the contents of TNF-α and IL-1β in each group compared with the model group; the units of the above four indicators are all ng / mL. The test results are shown in Table 4.

[0106] Table 4: Anti-inflammatory repair test

[0107]

[0108] According to the test results in Table 4, it can be seen that the composition prepared by the present invention has excellent anti-inflammatory and skin repair effects.

[0109] Combined with the test results of compositions 1, 5-7, it can be seen that the present invention uses the combination of ethyl acetate and chloroform as the organic solvent in the extractant. Compared with using one of them or replacing it with other organic solvents to extract Ganoderma atrum, the prepared Ganoderma atrum extract has better anti-inflammatory effects and can endow the composition with better anti-inflammatory and repair performance; the combined use of ethyl acetate and chloroform produces a mutually promoting extraction effect and improves the anti-inflammatory performance of the Ganoderma atrum extract.

[0110] Combined with the test results of compositions 1, 8-9, it can be seen that when the dosage of one of asiaticoside or Ganoderma atrum extract in compositions 8-9 is the same as the total amount of asiaticoside and Ganoderma atrum extract in composition 1, the anti-inflammatory effect of compositions 8-9 is worse than that of composition 1; thus, it can be seen that the present invention uses the combined action of asiaticoside and Ganoderma atrum extract to synergistically enhance the anti-inflammatory performance of the composition.

[0111] Combined with the test results of compositions 1, 12, it can be seen that when the mass ratio of asiaticoside to Ganoderma atrum extract in the present invention is 0.4-0.8:1, the prepared composition has better anti-inflammatory performance.

[0112] 3. Moisturizing and Skin Nourishing Test

[0113] Forty-two volunteers with healthy skin aged 30 - 50 were recruited and randomly divided into 7 groups, with 6 people in each group. Volunteers in each group used the above-mentioned compositions 1 - 4, 10 - 11, 13 on the face twice a day (once in the morning and once in the evening), with a dosage of 0.8 g each time, evenly applied on the face, and used continuously for 60 days. Before use and after 60 days of use, the water content of the stratum corneum of the volunteers' cheeks (unit: C.U.) was measured using CM825. Each test was conducted at 3 positions on the cheeks, and the average value was taken for summarization. Then, the increase in water content (unit: C.U.) on the 60th day compared with that before use was calculated. The results are shown in Table 5.

[0114] Table 5: Results of Moisturizing and Skin Nourishing Test

[0115]

[0116] According to the test in Table 5, it can be known that the composition prepared by the present invention can effectively improve the water content of the skin and has a good moisturizing and skin nourishing effect.

[0117] Combined with the tests of Compositions 1 and 4, it can be known that the moisturizing effect of the Saposhnikovia root extract prepared by extraction with a high-concentration ethanol solution is relatively poor. The present invention uses an ethanol aqueous solution with a concentration of 20 - 35 wt% to extract the Saposhnikovia root extract, which has better moisturizing performance.

[0118] Combined with the tests of Compositions 1, 10 - 11, it can be known that when the dosage of either the Saposhnikovia root extract or the filtrate of yeast fermented lysate in Composition 10 - 11 is the same as the total amount of the Saposhnikovia root extract and the filtrate of yeast fermented lysate in Composition 1, the moisturizing and skin nourishing effect of Composition 10 - 11 is worse than that of Composition 1. Thus, it can be seen that the present invention uses a combination of the Saposhnikovia root extract and the filtrate of yeast fermented lysate to promote each other and improve the moisturizing and skin nourishing performance of the composition.

[0119] Combined with the test results of Compositions 1 and 13, it can be known that when the mass ratio of the Saposhnikovia root extract to the filtrate of yeast fermented lysate is 1:1 - 1.5, the composition obtained has better moisturizing and skin nourishing performance.

[0120] Obviously, those skilled in the art can make various modifications and variations to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and its equivalent technologies, the present invention is also intended to include these modifications and variations.

Claims

1. A composition for repairing sensitive skin, characterized in that: The raw material composition is as follows: 0.001-0.2 parts of collagen, 0.001-0.1 parts of soluble collagen, 0.001-0.2 parts of hydrolyzed collagen, 0.05-1 parts of madecassoside, 0.005-0.04 parts of Albizzia Julibrissin extract, 0.05-1 parts of Saposhnikovia root extract, 0.05-0.8 parts of yeast fermentation lysate filtrate, 0.01-1 parts of Bifida yeast fermentation filtrate, 0.01-0.3 parts of Bacillus fermentation product, 0.001-0.1 parts of carnosine, 0.01-0.3 parts of Polygonum cuspidatum root extract, 0.05-1 parts of black ganoderma lucidum extract, 0.1-5 parts of butylene glycol and 5-50 parts of water; the mass ratio of Saposhnikovia root extract to yeast fermentation lysate filtrate is 1:1-1.5; The method for extracting the radix fangfeng extract comprises: crushing the radix fangfeng, then mixing it with a 20-35wt% ethanol aqueous solution at a mass ratio of 1:10-50, heating and stirring at 45-60°C for 5-12 hours, filtering to obtain a filtrate, and drying the filtrate to obtain the radix fangfeng extract; The extraction method of the black ganoderma lucidum extract comprises: crushing the black ganoderma lucidum to obtain a crushed material; adding a 50-75wt% ethanol aqueous solution with a mass of 10-30 times the mass of the crushed material, heating and stirring at 50-60°C for 2-5 hours, and then filtering to obtain a filtrate; concentrating the filtrate under reduced pressure at 50-60°C to a mass of 0.8-1.5 times the mass of the crushed material to obtain a concentrate; mixing the concentrate with an extractant composed of water and an organic solvent in a mass ratio of 1:2-4, the organic solvent being ethyl acetate and chloroform in a mass ratio of 2-3.5:1, and then stirring and extracting for 2-4 hours at a temperature of 30-40°C, and then standing to separate, and concentrating the oil phase obtained by separation under reduced pressure until no ethyl acetate and chloroform can be detected, and stopping the reduced pressure distillation to obtain the black ganoderma lucidum extract.

2. The composition according to claim 1, characterized in that The mass ratio of madecassoside to black ganoderma lucidum extract is 0.4-0.8:

1.

3. A method for preparing the composition according to any one of claims 1 to 2, characterized in that: The process comprises stirring and mixing yeast fermentation lysate filtrate, bifid yeast fermentation product filtrate, black ganoderma lucidum extract and radix fangfeng extract, then adding the remaining raw materials, continuing stirring and sterilizing to obtain the composition.

4. Use of the composition according to any one of claims 1 to 2 in the preparation of cosmetics, characterized in that: The amount of the composition in cosmetics is 0.1-30wt%.

5. The use according to claim 4, characterized in that: The dosage form of the cosmetic includes aqueous solution, emulsion, ointment, cream or powder.

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