A tropical plant composition with soothing and repairing effects, its preparation method and application

Abstract

This invention discloses a soothing and repairing tropical plant composition, its preparation method, and its application. The composition mainly comprises the following ingredients in parts by weight: 1-50 parts of Pandanus leaf extract, 1-50 parts of Desert Rose extract, 1-30 parts of Frangipani extract, 1-30 parts of Impatiens extract, and 1-30 parts of Verbena extract. The invention also discloses the preparation method and application of this composition. The preparation method includes steps such as low-temperature extraction, concentration and enrichment, material clarification, column chromatography adsorption, and low-temperature drying. This method can effectively enrich specific plant polysaccharides, total phenols, and other active ingredients, thereby inhibiting the release of inflammatory factors, clearing reactive oxygen species, and soothing and stabilizing the skin. When added as an active ingredient to daily chemical products, it can exert good soothing and repairing effects, stabilizing and balancing the skin from multiple dimensions, and has excellent results.

Description

Technical Field

[0001] This invention discloses a tropical plant composition with soothing and repairing effects, its preparation method and application, belonging to the field of cosmetic raw material preparation technology. Background Technology

[0002] With the introduction of new cosmetic regulations and the gradual standardization of efficacy claims in the industry, "efficacy" has become an increasingly important factor for products, leading to a surge in the popularity of active ingredients. However, while some active ingredients offer good efficacy, excessive addition or an overabundance of active ingredients in a product can easily trigger irritation in the formula, resulting in symptoms such as redness, swelling, irritation, and discomfort. Therefore, soothing ingredients specifically formulated for particular formulations and target groups are gaining attention. Developing raw materials with good soothing and repairing properties, based on the premise of safety and stability, has become a key focus for many companies and the entire industry.

[0003] Tropical plants refer to plants that live in tropical and subtropical regions. Tropics are characterized by small temperature variations and year-round summer, with an average annual temperature above 22-26℃. Due to the hot climate and abundant rainfall, tropical plants often possess rich bioactive components. Furthermore, due to their long-term growth under high ultraviolet radiation and high heat conditions, some tropical plants also produce more restorative and stabilizing components to cope with nature. This project draws inspiration from this, attempting to develop soothing and repairing ingredients primarily composed of tropical plant bioactive components, to be incorporated into daily chemical products to exert soothing and repairing effects.

[0004] CN117379354A discloses a repairing and soothing water containing rose extract and its preparation method. It contains rose hydrosol, moisturizer, antioxidant, skin conditioning agent and other ingredients. Although it has good soothing, anti-allergy and repairing effects, it is a single-formula product, not a raw material product, and its ingredients are relatively complex, which greatly limits its practical application.

[0005] CN109394615B discloses a soothing and repairing composition containing multiple plant extracts. The soothing and repairing agents include hydrolyzed lupin protein, carob fruit extract, and senna flower extract. Although it has a soothing and repairing effect, the extraction process of the plant repairing ingredients is not described, which may cause differences in actual use.

[0006] Therefore, it is essential to develop a plant composition derived from tropical plants with soothing and repairing effects, and to incorporate it as an active ingredient into daily chemical products to achieve soothing and repairing effects such as calming the skin, reducing skin irritation, and protecting the skin barrier. Summary of the Invention

[0007] To address the aforementioned shortcomings, the present invention aims to provide a tropical plant composition with soothing and repairing effects. This composition has excellent soothing and repairing effects and can be used in cosmetics to effectively remove excess free radicals, inhibit the release of skin inflammatory factors, and repair the skin barrier. Moreover, the plant composition itself is safe, gentle, and non-irritating, and can be widely used in the skin care field.

[0008] Therefore, the first technical solution provided by this invention is as follows:

[0009] A tropical plant composition with soothing and repairing effects, comprising the following ingredients in parts by weight: 1-50 parts of Pandanus leaf extract, 1-50 parts of Desert Rose extract, 1-30 parts of Frangipani extract, and 1-30 parts of Impatiens extract.

[0010] Furthermore, it also includes 1-30 parts of verbena extract.

[0011] Preferably, the product consists of the following ingredients in parts by weight: 1-50 parts Pandanus leaf extract, 1-50 parts Desert rose extract, 1-30 parts Frangipani extract, 1-30 parts Impatiens extract, and 1-30 parts Verbena extract.

[0012] All the plants used in this invention are tropical plants, containing abundant active plant components, which are briefly described below:

[0013] Pandanus leaves, possibly native to the Maluku Islands of Indonesia, are mainly cultivated in Sri Lanka, Malaysia, Thailand, and other countries. They are also cultivated in Hainan, my country. They thrive in tropical, humid, acidic soil. Rich in polysaccharides, volatile oils, and phenols, they can be used as food flavorings.

[0014] Desert roses, native to Africa, thrive in hot, dry, and sunny environments. Rich in anthocyanins, polyphenols, and volatile oils, they possess excellent antioxidant properties and have numerous medicinal and ornamental uses.

[0015] Frangipani, belonging to the Apocynaceae family, is native to tropical regions of the Americas, including Mexico, Venezuela, and the West Indies, and is now widely cultivated in subtropical regions. It contains flavonoids and polysaccharides, and has heat-clearing and dampness-removing effects.

[0016] Impatiens: A herbaceous plant belonging to the genus Impatiens in the family Balsaminaceae. It is native to India, Malaysia, and China. It contains various anthocyanins, flavonoids, etc., and has the effects of promoting blood circulation, relieving swelling, and antibacterial properties.

[0017] Verbena: A perennial herb belonging to the Verbenaceae family and the Verbena genus, widely distributed, mainly produced in tropical America, growing at the foot of mountains, along roadsides, or in wastelands near villages. The whole plant is used medicinally, possessing the effects of clearing heat and detoxifying, promoting blood circulation and removing blood stasis, and promoting diuresis and reducing swelling.

[0018] In the plant extract composition of this invention, Pandanus leaf extract and desert rose extract are the first active ingredients, mainly composed of plant polysaccharides, organic acids, and vitamins. They can form a film on the skin surface, providing moisturizing and repairing effects on the skin barrier. Frangipani, Impatiens balsamina, and Verbena officinalis are the second active ingredients, mainly composed of plant polyphenols and flavonoids, which inhibit the release of inflammatory factors and scavenge excess free radicals. The combination of these two ingredients, along with low-temperature extraction for preservation and freeze-drying, allows for synergistic effects, maximizing the soothing and repairing properties, and has promising application prospects in the cosmetics industry.

[0019] The second technical solution provided by this invention is:

[0020] A method for preparing a tropical plant composition with soothing and repairing effects includes the following steps:

[0021] S1. Preparation of component A: Weigh dried Pandanus leaves and rose petals, crush them with a pulverizer and mix them evenly, add extraction solvent, and extract twice at low temperature, 1-3 hours each time. After extraction, filter with a 400-mesh filter cloth to remove the filter residue, collect and combine the two extracts, concentrate under reduced pressure to the same mass as weighed, and microfilter the filtrate to obtain component A.

[0022] S2. Preparation of Component B: Weigh the dried frangipani, balsam, and verbena, pulverize them using a traditional Chinese medicine pulverizer and mix them evenly. Add the extraction solvent and extract twice at low temperature, 1-3 hours each time. After extraction, filter through a 400-mesh filter cloth to remove the filter residue and collect and combine the two extracts. Concentrate the obtained extract under reduced pressure to 3 times the mass of the original plant, microfilter and collect the filtrate. Then add the filtrate to a macroporous adsorption resin for column adsorption at an adsorption flow rate of 1 BV / h. After adsorption, wash with water for 3 BV, then elute with 3 BV of ethanol aqueous solution. Collect the eluent and concentrate it under reduced pressure to the same mass as the weighed sample to obtain the concentrated solution, which is component B.

[0023] S3. Low-temperature drying: Mix components A and B in equal amounts at a 1:1 ratio, stir evenly, add excipients, place in a freeze dryer for pre-freezing, then sublime dry, and pulverize to obtain the final plant composition.

[0024] Preferably, the extraction solvent in S1 is pure water, and the material-to-liquid ratio is 1:5-1:30 (g / ml).

[0025] Preferably, the extraction solvent in S2 is 30-95% ethanol-water (v / v), and the material-to-liquid ratio is 1:10-1:30 (g / ml).

[0026] Preferably, the low temperature range in S1 and S2 is 30℃-60℃.

[0027] Preferably, the extraction methods in S1 and S2 are any one of constant temperature stirring extraction, ultrasonic extraction, and microwave extraction.

[0028] Preferably, the pore size of the microfiltration membranes in S1 and S2 is 0.20-0.45 μm.

[0029] Preferably, the macroporous adsorption resin in S2 is a non-polar or weakly polar macroporous resin with styrene-divinylbenzene as its backbone.

[0030] Preferably, the volume fraction of ethanol in the ethanol aqueous solution of the eluent in S2 is 40%-70%.

[0031] Preferably, the excipient in S3 is starch or maltodextrin.

[0032] Preferably, in step S3, the temperature of the freeze-drying pre-freezing stage is -30 to -70°C, and the freezing time is 4-8 hours; the sublimation stage is controlled at a pressure of 0-20 Pa for 10-20 hours.

[0033] The third technical solution provided by the present invention is: the application of the plant composition with soothing and repairing properties, wherein the plant composition is applied to soothing and repairing cosmetics.

[0034] Preferably, the soothing and repairing cosmetic is a topical skin preparation made by using the composition with soothing and repairing effects as the active ingredient, plus conventional pharmaceutical or cosmetic excipients or auxiliary ingredients.

[0035] Specifically, the soothing and repairing cosmetic is one or more of the following: toner, serum, cream, mask, and gel.

[0036] Compared with the prior art, the present invention has the following beneficial effects:

[0037] 1. The plant composition with soothing and repairing properties disclosed in this invention uses different extraction processes designed based on the characteristics of plants to extract specific plant polysaccharides, organic acids, and specific plant polyphenols, flavonoids, etc., which can effectively remove excess free radicals, inhibit the release of skin inflammatory factors, and repair the skin barrier, thus having excellent soothing and repairing effects.

[0038] 2. The plants selected in this invention are all derived from specific tropical plants, which are rich in plant active ingredients. At the same time, they are scientifically formulated to have a synergistic effect and better promote the efficacy.

[0039] 3. The extraction and purification process of this invention uses a temperature of no more than 60°C throughout the entire process, and the drying process is low-temperature freeze drying, which preserves the active ingredients of the plant to a greater extent and prevents them from being damaged by heat.

[0040] 4. The plant composition with soothing and repairing properties disclosed in this invention has the advantages of being all-natural, non-irritating, safe and non-toxic, which can meet the needs of safe skin care and has good promotion prospects and a broad application market. Detailed Implementation

[0041] Numerous specific details are set forth in the following description to provide a full understanding of the invention. However, the invention can be practiced in many other ways different from those described herein, and similar modifications can be made by those skilled in the art without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0042] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

[0043] It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0044] In the embodiments, unless otherwise specified, all methods used are conventional methods in the art.

[0045] As used herein, the terms “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion.

[0046] For example, a composition, step, method, article, or apparatus that includes the listed elements is not limited to those elements, but may include other elements not expressly listed or elements inherent to such a composition, step, method, article, or apparatus.

[0047] Furthermore, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0048] The reagents and instruments used in the embodiments and comparative examples of this invention are all commercially available products.

[0049] The claims of the present invention will be further described in detail below with reference to specific embodiments, but this does not constitute any limitation on the present invention. Any limited modifications made by any person within the scope of protection of the claims of the present invention are still within the scope of protection of the claims of the present invention.

[0050] Example 1

[0051] A tropical plant composition with soothing and repairing effects, comprising the following components: 20g of Pandanus leaf, 20g of rose, 20g of frangipani, 20g of balsam, and 20g of verbena.

[0052] Example 2

[0053] A tropical plant composition with soothing and repairing effects, comprising the following components: 50g of Pandanus leaf, 1g of rose, 30g of frangipani, 10g of balsam, and 9g of verbena.

[0054] Example 3

[0055] A tropical plant composition with soothing and repairing effects, comprising the following components: 1g of Pandanus leaf, 50g of rose, 30g of frangipani, 9g of balsam, and 10g of verbena.

[0056] Example 4

[0057] A tropical plant composition with soothing and repairing effects, comprising the following components: 25g of Pandanus leaf, 25g of rose, 1g of frangipani, 30g of Impatiens, and 19g of Verbena.

[0058] Example 5

[0059] A tropical plant composition with soothing and repairing effects, comprising the following components: 10g of Pandanus leaf, 30g of rose, 29g of frangipani, 1g of balsam, and 30g of verbena.

[0060] Example 6

[0061] A tropical plant composition with soothing and repairing effects, comprising the following components: 30g of Pandanus leaf, 20g of rose, 20g of frangipani, 29g of balsam, and 1g of verbena.

[0062] Example of preparation:

[0063] S1. Preparation of Component A: Accurately weigh the dried leaves of Pandanus odoratus and rose petals according to the above mass ratio, pulverize them with a Chinese medicine pulverizer and mix them evenly. Add pure water at a material-to-liquid ratio of 1:20 (g / ml) for extraction. Perform constant temperature stirring extraction at 50℃, extract twice, 2 hours each time. After extraction, filter with a 400-mesh filter cloth to remove the filter residue. Collect and combine the two extracts, concentrate under reduced pressure to the equivalent of the plant mass, and microfilter with a 0.45μm pore size to obtain the filtrate, which is Component A.

[0064] S2. Preparation of Component B: Accurately weigh dried frangipani, balsam, and verbena according to the above mass ratio, pulverize and mix them evenly using a traditional Chinese medicine pulverizer, add 60% ethanol-water (v / v) at a material-to-liquid ratio of 1:30 (g / ml), and extract twice with ultrasound at 60℃ for 1 hour each time. After extraction, filter through a 400-mesh filter cloth to remove the filter residue, and collect and combine the two extracts. Concentrate the obtained extract under reduced pressure to 3 times the original plant mass, and microfilter with a 0.22μm pore size to obtain the filtrate. Then add the filtrate to a macroporous adsorption resin for column adsorption at an adsorption flow rate of 1 BV / h. After adsorption, wash with water for 3 BV, and then elute with 3 BV of 50% ethanol-water (v / v) solution. Collect the eluent, and then concentrate under reduced pressure to the same mass as the plant to obtain the concentrated solution, which is component B.

[0065] S3. Low-temperature drying: Mix components A and B in equal amounts at a 1:1 ratio, stir evenly, add malt starch, and pre-freeze in a freeze dryer at -50℃ for 6 hours. Then, sublime dry in an environment with an air pressure of 2Pa for 15 hours. After drying, pulverize to obtain the final plant composition.

[0066] To demonstrate the effectiveness of this invention, comparative experimental examples are provided below:

[0067] The preparation methods of any one of the compositions in Comparative Examples 1-5 are the same as those in the examples.

[0068] Comparative Example 1

[0069] A tropical plant composition with soothing and repairing effects, comprising the following components: 40g of Pandanus leaf, 20g of rose petals, 20g of frangipani, and 20g of Impatiens balsamina.

[0070] Comparative Example 2

[0071] A tropical plant composition with soothing and repairing effects, comprising the following components: 30g rose petals, 30g frangipani, 30g impatiens, and 10g verbena.

[0072] Comparative Example 3

[0073] A tropical plant composition with soothing and repairing effects, comprising the following components: 20g of Pandanus leaf, 40g of rose, 30g of Impatiens flower, and 10g of Verbena.

[0074] Comparative Example 4

[0075] A tropical plant composition with soothing and repairing effects, comprising the following components: 10g of Pandanus leaf, 30g of rose petals, 30g of frangipani, and 30g of verbena.

[0076] Comparative Example 5

[0077] A tropical plant composition with soothing and repairing effects, comprising the following components: 30g of Pandanus leaf, 20g of Frangipani flower, 30g of Impatiens flower, and 20g of Verbena officinalis.

[0078] The above-mentioned plant-based compositions with soothing and repairing effects can be used to prepare soothing and repairing cosmetics. These soothing and repairing cosmetics are topical skin preparations made by using the compositions with soothing and repairing effects as active ingredients, along with conventional pharmaceutical or cosmetic excipients or auxiliary ingredients. In specific use, the compositions prepared according to this invention are directly added to the cosmetic matrix; the amount added can be adjusted according to actual needs, preferably 0.1 wt% to 5 wt%.

[0079] To better illustrate the advantages of the present invention, the following are efficacy experiments of the composition with soothing and repairing effects provided by the present invention:

[0080] Experiment 1: Antioxidant Test – ABTS Free Radical Scavenging Experiment

[0081] The soothing and repairing compositions prepared in Experimental Examples 1-6 and Comparative Examples 1-5 were tested. The tests are as follows:

[0082] 1. Preparation of ABTS+ standard stock solution:

[0083] Accurately weigh 0.0384 g of ABTS+, dissolve it in distilled water, and dilute to a volumetric flask with distilled water to obtain an ABTS+ concentration of 7 mmol·L⁻¹. -1 Accurately weigh 0.0066 g of K₂S₂O₈ (potassium persulfate), dissolve it in distilled water, and dilute to a volumetric flask with distilled water to obtain a K₂S₂O₈ concentration of 2.45 mmol·L⁻¹. -1 Then, mix the two solutions in a brown bottle (1:1 ratio) and leave it in the dark at room temperature overnight (12-16 hours) to obtain an ABTS+ solution.

[0084] ABTS+ solution was diluted with phosphate buffer (10 mmol·L⁻¹). -1 Dilute the solution (pH 7.4) by approximately 25 times until its absorbance at 734 nm wavelength and 30 °C is 0.700 ± 0.020, thus obtaining the ABTS+ working solution.

[0085] 2. Sample clearance rate test

[0086] Take 1 mL of the same diluted sample solution into each test tube, add 3 mL of ABTS+ working solution, mix thoroughly, and store at room temperature in the dark for 10 min. Measure the absorbance at 734 nm, using anhydrous ethanol as a blank control. Vitamin C is also included as a control. Calculate the ABTS+ scavenging rate of each extract using the following formula: ABTS+ scavenging rate is calculated as follows:

[0087] ABTS radical scavenging rate (%) = [1 - (Ai - Aj) / Ac] × 100%

[0088] In the formula: Ai is 1 mL of sample solution + 3 mL of ABTS + The absorbance value of the solution mixture; Aj is the absorbance value of the mixture of 1 mL sample solution + 3 mL anhydrous ethanol; Ac is the absorbance value of the mixture of 1 mL anhydrous ethanol + 3 mL ABTS. + The absorbance value of the solution mixture.

[0089] Table 1. Experimental results of the composition's ability to scavenge ABTS free radicals.

[0090] Sample Name ABTS free radical scavenging rate Vitamin C 93.21％ Example 1 87.63％ Example 2 82.26％ Example 3 91.05％ Example 4 80.17％ Example 5 88.63％ Example 6 81.63％ Comparative Example 1 62.82％ Comparative Example 2 72.54％ Comparative Example 3 76.33％ Comparative Example 4 68.29％ Comparative Example 5 57.10％

[0091] Table 1 shows that the control vitamin C scavenged 93.21% of ABTS free radicals, while Example 3 showed an inhibition rate of 91.05%, indicating that the inhibition rates were quite similar. The scavenging effects of other examples were also all above 80%, demonstrating that this soothing and repairing composition has a good scavenging effect on ABTS free radicals. Furthermore, the table shows that the scavenging rates of Examples 1-6 were all higher than those of Comparative Examples 1-5, indicating that the antioxidant capacity of the composition containing five plant extracts is superior to that of the composition lacking any plant extract. The five plants have a synergistic effect, and the formulation is well-matched, indicating that this composition has good antioxidant capacity, can protect the skin through certain pathways, and contributes to the soothing effect.

[0092] Experiment 2: Macrophage Inflammation Model Experiment

[0093] The soothing and repairing compositions prepared in Examples 1-6 and Comparative Examples 1-5 were tested as follows:

[0094] 1. Cytotoxicity assay: RAW264.7 cell suspension was adjusted to different concentrations and added to 96-well cell culture plates. The plates were incubated overnight at 37°C with 5% CO2 for assay. Test samples were diluted with cell culture medium to different concentrations and added to cell culture plates. The plates were incubated at 37°C with 5% CO2 for 20–48 h. The culture medium was discarded, and the cells were washed 1–2 times with PBS. MTT working solution was added, and the cells were incubated for further incubation. After incubation, the culture medium was discarded, and DMSO was added. The absorbance was measured at 570 nm (with a background of 630 nm). Cytotoxicity was calculated based on the results, and, combined with cell morphology analysis, sample concentrations with no significant cytotoxicity were selected for further experiments.

[0095] 2. IL-6 Assay: RAW264.7 cell suspension was adjusted to a non-cytotoxic concentration and added to 96-well cell plates. The cells were incubated overnight at 37°C with 5% CO2 for detection. The test samples were diluted with LPS-containing cell culture medium to a non-cytotoxic concentration before addition. LPS-containing medium served as the negative control (NC), and LPS-free cell culture medium served as the blank control (BC). A positive control (PC, dexamethasone sodium phosphate) diluted with LPS-containing cell culture medium was used as the positive control. Cells were cultured for 20–24 hours after addition. After the assay, the supernatant was collected, and the IL-6 ELISA kit was used for detection.

[0096] Result calculation and judgment:

[0097] Formula for calculating the downregulation rate of the inflammatory factor IL-6:

[0098] Downward adjustment rate = (1-T / NC)×100%

[0099] in:

[0100] T – Average value of inflammatory factor IL-6 in the experimental group

[0101] NC – Mean value of inflammatory factor IL-6 in the negative control group

[0102] Perform statistical and significance analysis on the data, calculate the P-value, P < 0.05 indicates a significant difference, otherwise there is no statistical difference.

[0103] When the IL-6 content of the inflammatory factor in the negative control group was much higher than that in the blank control group, and the difference between the two was statistically significant (P < 0.05), the experiment was considered successful.

[0104] When the level of the inflammatory factor IL-6 in the sample group was lower than that in the negative control group, and the difference between the two was statistically significant (P < 0.05), the sample could be considered to have a soothing effect.

[0105] Table 2. Experimental Results of the Composition's Inhibition of Inflammatory Factors

[0106]

[0107]

[0108] Note: If P > 0.05, it indicates no significant difference, denoted as "ns"; if P < 0.05, it indicates a significant difference, and 0.01 < P ≤ 0.05, denoted as "*"; 0.001 < P ≤ 0.01, denoted as "**"; 0.0001 < P ≤ 0.001, denoted as "***"; P ≤ 0.0001, denoted as "****".

[0109] The results of the inhibition experiments on the inflammatory factor IL-6 content of Examples 1-6 and Comparative Examples 1-5 are shown in Table 2 above. BC is the experimental blank control, NC is the experimental negative control, and PC is the positive control. The IL-6 content in the NC group was significantly higher than that in the blank control group (P < 0.05), indicating the validity of the experiment. As shown in the table, when the same safe concentration of the examples and comparative examples was added, Example 2 showed the best effect, with a downregulation rate of 52.30% for IL-6 inflammatory factor induced by exogenous stimulation, which was significantly different from the negative control group (P < 0.05) and close to the downregulation rate of the positive control PC. Furthermore, the downregulation rate of cellular inflammatory factors in all examples was above 30%, which was better than any of the comparative examples, further demonstrating the rationality of this formulation and its synergistic effect. This also indicates that this soothing and repairing composition can effectively inhibit the release of the inflammatory factor IL-6, exhibiting a good soothing effect in this pathway.

[0110] Experiment 3: Test on the Promotion of Caudal Fin Repair in Zebrafish Embryos

[0111] 1. Experimental Principle

[0112] When the skin barrier is damaged, the body needs to accelerate the growth rate of epidermal cells to repair the damaged skin barrier. The process and principle of skin barrier repair in zebrafish and humans are very similar, making zebrafish a suitable model for screening and testing the efficacy of human skin repair. This experiment created a caudal fin damage model by removing the caudal fin of zebrafish, and evaluated the effect of drug administration on caudal fin repair, providing data support for the sample's function in promoting skin repair.

[0113] 2. Experimental Methods

[0114] Step 1: Select healthy zebrafish embryos that are 3 days old after fertilization;

[0115] Step 2: Remove the caudal fin of the zebrafish embryo to establish a damage model;

[0116] Step 3: Different groups were set up and incubated for 48 hours after sample addition;

[0117] Step 4: Take a photograph under a fluorescence microscope or stereomicroscope;

[0118] Step 5: Data processing and analysis.

[0119] 3. Results and Judgments

[0120] Caudal fin repair promotion rate: Promotion rate = (T – C) / C × 100%

[0121] Where: T—the average length of the caudal fin of the fish embryos in the test group; C—the average length of the caudal fin of the fish embryos in the model control group.

[0122] A two-tailed t-test was performed to compare the caudal fin repair levels of embryos in the test group and the model control group to obtain the p-value. P < 0.05 indicated a statistically significant difference; otherwise, there was no statistically significant difference.

[0123] The experiment was considered valid if at least 90% of the fish embryos survived after exposure in each test group, and the tail fin repair promotion rate of the positive control group fish embryos was positive and P < 0.05.

[0124] When the promotion rate of the test substance treatment group is positive and there is a statistically significant difference compared with the model control group (P < 0.05), the sample can be considered to have the effect of promoting tail fin repair.

[0125] Examples 1-6 and Comparative Examples 1-5 were diluted to the same safe concentration and zebrafish tail fin repair experiments were conducted. The results are shown in Table 3.

[0126] Table 3. Results of the test on promoting tail fin repair in zebrafish embryos

[0127] Group average tail fin length Repair Promotion Rate p-value Significance Model control group 75.28±15.34 / / / Positive control group 102.54±14.66 36.21％ ＜0.0001 **** Example 1 97.80±20.32 29.91％ ＜0.0001 **** Example 2 92.64±14.08 23.06％ 0.0003 *** Example 3 95.33±12.34 26.63％ ＜0.0001 **** Example 4 88.26±20.58 17.24％ ＜0.0001 **** Example 5 85.37±17.20 18.40％ ＜0.0001 **** Example 6 90.54±21.06 20.27％ 0.0003 *** Comparative Example 1 82.24±11.03 9.25％ 0.0027 ** Comparative Example 2 79.45±17.65 5.54％ ＜0.0001 **** Comparative Example 3 74.32±24.65 -1.28％ 0.0526 ns Comparative Example 4 79.56±10.23 5.69％ 0.008 ** Comparative Example 5 75.65±12.34 0.49％ ＜0.0001 ****

[0128] Note: If P > 0.05, it indicates no significant difference, denoted as "ns"; if P < 0.05, it indicates a significant difference, and 0.01 < P ≤ 0.05, denoted as "*"; 0.001 < P ≤ 0.01, denoted as "**"; 0.0001 < P ≤ 0.001, denoted as "***"; P ≤ 0.0001, denoted as "****".

[0129] In this zebrafish embryo tail fin repair experiment, over 90% of the fish embryos survived after exposure in the test group, and the tail fin repair promotion rate in the positive control group was positive at 36.21% (P < 0.05), indicating the effectiveness of the experiment. Table 3 shows that among the samples in Examples 1-6 and Comparative Examples 1-5, Example 1 showed the best repair effect on zebrafish embryo tail fins, with a repair promotion rate of 29.91%, slightly lower than the 36.21% repair promotion rate in the positive control group, indicating a good repair effect. Furthermore, data analysis shows that the samples in Examples 1-6 all showed good repair promotion rates for zebrafish embryo tail fins, all above 17%, and better than any of the comparative examples, further demonstrating the rationality of this formulation and the synergistic effect of the five plant combinations. This also indicates that this soothing and repairing composition can effectively promote the repair of fish embryo tail fins and has good potential repair effects when applied in cosmetics.

[0130] In summary, this invention uses tropical plants as its source, scientifically combining Pandanus leaves, desert rose, frangipani, balsam, and verbena. It employs extraction and purification processes such as low-temperature extraction, concentration and enrichment, material clarification, column chromatography adsorption, and low-temperature drying to selectively enrich the plant's active ingredients, thus obtaining a soothing and repairing composition. This composition effectively inhibits the release of inflammatory factors, eliminates reactive oxygen species, and soothes and stabilizes the skin. When added to daily chemical products, it provides excellent soothing and repairing effects.

[0131] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A tropical plant composition with soothing and repairing effects, characterized in that, It includes the following ingredients in parts by weight: Pandanus leaf extract 1-50 parts, Desert rose extract 1-50 parts, Frangipani extract 1-30 parts, Impatiens flower extract 1-30 parts, and Verbena extract 1-30 parts. The method for preparing the composition includes the following steps: S1. Preparation of component A: Weigh dried Pandanus leaves and rose petals, crush them with a pulverizer and mix them evenly, add extraction solvent, and extract twice at low temperature, 1-3 hours each time. After extraction, filter with a 400-mesh filter cloth to remove the filter residue, collect and combine the two extracts, concentrate under reduced pressure to the same mass as weighed, and microfilter the filtrate to obtain component A. S2. Preparation of Component B: Weigh the dried frangipani, balsam, and verbena, pulverize them using a traditional Chinese medicine pulverizer and mix them evenly. Add the extraction solvent and extract twice at low temperature, 1-3 hours each time. After extraction, filter through a 400-mesh filter cloth to remove the filter residue and collect and combine the two extracts. Concentrate the obtained extract under reduced pressure to 3 times the mass of the original plant, microfilter and collect the filtrate. Then add the filtrate to a macroporous adsorption resin for column adsorption at an adsorption flow rate of 1 BV / h. After adsorption, wash with water for 3 BV, then elute with 3 BV of ethanol aqueous solution. Collect the eluent and concentrate it under reduced pressure to the same mass as the weighed sample to obtain the concentrated solution, which is component B. S3. Low-temperature drying: Components A and B are mixed in equal proportions (1:1), stirred until homogeneous, and then excipients are added. The mixture is pre-frozen in a freeze dryer, followed by sublimation drying and pulverization to obtain the final plant composition. The extraction solvent in S1 is pure water, the extraction solvent in S2 is 30-95% ethanol-water (v / v), and the macroporous adsorption resin in S2 is a non-polar or weakly polar macroporous resin with styrene-divinylbenzene as the backbone.

2. The composition according to claim 1, characterized in that, It consists of the following ingredients in parts by weight: 1-50 parts Pandanus leaf extract, 1-50 parts Desert rose extract, 1-30 parts Frangipani extract, 1-30 parts Impatiens flower extract, and 1-30 parts Verbena extract.

3. The composition according to claim 1, characterized in that, The material-to-liquid ratio in S1 is 1:5-1:30 (g / ml).

4. The composition according to claim 1, characterized in that, The material-to-liquid ratio in S2 is 1:10-1:30 (g / ml).

5. The composition according to claim 1, characterized in that, The low temperature range of S1 and S2 is 30℃-60℃, and the extraction method of S1 and S2 is any one of constant temperature stirring extraction, ultrasonic extraction, and microwave extraction. The pore size of the microfiltration membrane in S1 and S2 is 0.20-0.45μm.

6. The composition according to claim 1, characterized in that, The ethanol-water eluent in S2 has a volume fraction of 40%-70%.

7. The composition according to claim 1, characterized in that, The excipient in S3 is starch or maltodextrin. The temperature of the freeze-drying pre-freezing stage in S3 is -30 to -70°C, and the freezing time is 4-8 hours. The sublimation stage is controlled at a pressure of 0-20 Pa for 10-20 hours.

8. The use of a composition according to any one of claims 1-7, characterized in that, The composition is used in the preparation of cosmetics, which are topical skin preparations made by using the composition as the active ingredient and adding conventional pharmaceutical or cosmetic excipients.

Images