A sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia and its preparation method

Abstract

The present invention provides a sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia and a preparation method thereof, belonging to the technical field of traditional Chinese medicine. The method of the present invention is first made by processing and extracting Ganoderma lucidum and then adding a flavoring agent, which is suitable for diabetic patients to nourish the heart and soothe the mind, strengthen the spleen and harmonize the stomach, and is used for the treatment of palpitation, insomnia, loss of appetite and neurasthenia. The Ganoderma lucidum syrup of the present invention has good efficacy and is sugar-free.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and particularly to a preparation method of sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia. Background Art

[0002] Ganoderma lucidum is the fruiting body of the fungus Ganoderma lucidum in the family Polyporaceae, and has the effects of replenishing qi and calming the mind, relieving cough and asthma. It is used for restlessness of mind, insomnia and palpitation, cough and asthma due to lung deficiency, general weakness and shortness of breath, and loss of appetite. It is commonly used for dizziness and insomnia, palpitation and shortness of breath, neurasthenia, and general weakness with cough. The fruiting body of Ganoderma lucidum is an umbrella-shaped mushroom, purple-red or brown-red in color, with a fleshy texture when young and becoming corky after drying when mature. The fruiting body consists of a pileus (cap) and a stipe. The Ganoderma lucidum described from "Shennong Ben Cao Jing" to "Pharmacopoeia of the People's Republic of China" refers to the fruiting body of Ganoderma lucidum. The chemical constituents of the Ganoderma genus are relatively complex, and the active ingredients can be divided into ten categories, including Ganoderma lucidum polysaccharides, Ganoderma lucidum polypeptides, triterpenoids, 16 amino acids (including seven essential amino acids for the human body), proteins, steroids, mannitol, coumarin glycosides, alkaloids, organic acids (mainly containing fumaric acid), and trace elements such as Ge, P, Fe, Ca, Mn, Zn, etc.

[0003] Ganoderma lucidum polysaccharide is one of the main active ingredients of Ganoderma lucidum, and has anti-tumor, immunomodulatory, hypoglycemic, antioxidant, hypolipidemic and anti-aging effects. There are many types of Ganoderma lucidum polysaccharides, about more than 200 kinds, and Ganoderma lucidum polysaccharides can be extracted from the fruiting body, spore powder and fermentation broth of Ganoderma lucidum.

[0004] Ganoderma lucidum syrup is a syrup preparation made from Ganoderma lucidum, and has the functions of nourishing the heart and calming the mind, strengthening the spleen and regulating the stomach. It is used for palpitations, insomnia, loss of appetite, and adjuvant treatment of neurasthenia, hyperlipidemia, coronary heart disease, and chronic bronchitis. A large amount of sucrose is added to conventional Ganoderma lucidum syrup, which is not suitable for people with low sugar requirements, and the existing Ganoderma lucidum syrup has a single composition and simple efficacy.

[0005] Therefore, preparing a sugar-free Ganoderma lucidum syrup is the technical problem to be solved at present. Summary of the Invention

[0006] In view of this, the present invention provides a preparation method of sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia, taking Ganoderma lucidum extract as the main raw material to improve the efficacy of Ganoderma lucidum syrup.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A preparation method of sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia, comprising the following steps:

[0009] (1)Crush Ganoderma lucidum to obtain coarse Ganoderma lucidum powder, add ethanol solution to submerge the coarse Ganoderma lucidum powder, moisten it, seal it, and let it stand for more than 1 hour to fully expand; evenly load the moist and expanded coarse Ganoderma lucidum powder into a percolator, and add ethanol solution 5 - 10 cm higher than the coarse Ganoderma lucidum powder, cover the percolator, and macerate for one week;

[0010] (2)After maceration, perform percolation. When the percolate becomes colorless, end the percolation, and collect the percolate and Ganoderma lucidum residue;

[0011] (3)Add water to the Ganoderma lucidum residue, heat to boiling for 2 hours, and collect the medicinal liquid; then add water to the residue again, heat to boiling for 2 hours and collect the medicinal liquid; combine the two medicinal liquids to obtain the water extract of Ganoderma lucidum;

[0012] (4)Combine the water extract of Ganoderma lucidum in step (3) with the percolate in step (2) to obtain the Ganoderma lucidum extract, then concentrate the Ganoderma lucidum extract and recover ethanol to obtain a concentrated solution;

[0013] (5)Heat purified water to boiling, add a flavoring agent, continuously heat and stir until the flavoring agent is completely dissolved, boil for 20 - 30 minutes to obtain a flavoring agent slurry, and cool it to below 70 °C;

[0014] (6)Mix the Ganoderma lucidum concentrated solution with the flavoring agent slurry, cool it to 60 - 70 °C, add sodium benzoate and water, mix well to obtain a premixed solution; then let the premixed solution stand, filter it with a 400 - mesh filter cloth to obtain the Ganoderma lucidum syrup.

[0015] Preferably, the particle size of the coarse Ganoderma lucidum powder in step (1) is 50 - 500 μm; the mass concentration of the ethanol is 80 ± 5%.

[0016] Preferably, during the percolation process in step (2), the ethanol is 5 - 10 cm higher than the drug surface, and the flow rate of the percolate is 3 - 5 mL / min.

[0017] Preferably, the first water addition amount for decocting with water in step (3) is 3 - 5 times the weight of the coarse Ganoderma lucidum powder, and the second water addition amount is 2 - 3 times the weight of the coarse Ganoderma lucidum powder.

[0018] Preferably, during the process of recovering ethanol in step (4), the vacuum degree is -0.03 MPa and the temperature is 80 ± 5 °C; during the concentration process, the vacuum degree is -0.05 MPa and the temperature is 70 °C ± 5 °C.

[0019] Preferably, the relative density of the concentrated solution in step (4) at 60 °C is 1.16 - 1.18.

[0020] Preferably, the flavoring agent in step (5) is any one of xylitol, oligosaccharide, stevioside, and Momordica grosvenori extract.

[0021] Preferably, the mass ratio of the purified water to the flavoring agent in step (5) is 12-18:35-45.

[0022] Preferably, the mass concentration of sodium benzoate in the premixed solution in step (6) is 0.2-0.3%, and the mass concentration of the flavoring agent is 45%-60%.

[0023] Another object of the present invention is to provide a sugar-free Ganoderma lucidum syrup prepared by the above method for treating neurasthenia and insomnia.

[0024] Compared with the prior art, the present invention has the following beneficial effects: The present invention provides a sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia and a preparation method thereof, which is first prepared by adding a flavoring agent after Ganoderma lucidum is processed, extracted and concentrated. The Ganoderma lucidum syrup of the present invention is suitable for diabetic patients to nourish the heart and soothe the nerves, strengthen the spleen and harmonize the stomach, and is used for the treatment of palpitations, insomnia, loss of appetite and neurasthenia. The Ganoderma lucidum syrup of the present invention has good efficacy and is sugar-free. Detailed implementation manners

[0025] The present invention provides a preparation method of a sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia, and the steps are as follows:

[0026] (1) Crush Ganoderma lucidum to obtain Ganoderma lucidum coarse powder with a particle size of 50-500 μm, add an ethanol solution with a mass concentration of 80% ± 5% to submerge the Ganoderma lucidum coarse powder, moisten and seal it for 1 hour to make it fully expand; evenly load the moist and expanded Ganoderma lucidum coarse powder into a percolator, apply appropriate pressure to make the Ganoderma lucidum coarse powder loose and uniform, the addition amount of the Ganoderma lucidum coarse powder does not exceed 2 / 3 of the percolation, and add an ethanol solution 5-10 cm higher than the Ganoderma lucidum coarse powder, cover the percolator, and soak for 7 days;

[0027] (2) After the impregnation is completed, carry out percolation. When the percolate becomes colorless, the percolation ends, and collect the percolate and the Ganoderma lucidum medicinal residues; during the percolation process, it is necessary to always keep the ethanol 5-10 cm higher than the medicinal materials to prevent the medicinal materials from drying and cracking, and it is necessary to timely supplement ethanol into the percolator and regularly check the percolation speed; the flow rate of the percolate is 3-5 mL / min;

[0028] (3) Add 3-5 times the weight of the Ganoderma lucidum coarse powder of water to the Ganoderma lucidum medicinal residues, heat to boiling for 2 hours, and collect the medicinal liquid; then add 2-3 times the weight of the Ganoderma lucidum coarse powder of water to the medicinal residues again, heat to boiling for 2 hours and collect the medicinal liquid; combine the two medicinal liquids to obtain a Ganoderma lucidum water extract;

[0029] (4) Combine the Ganoderma lucidum water extract in step (3) with the percolate in step (2) to obtain a Ganoderma lucidum extract, and then concentrate the Ganoderma lucidum extract and recover ethanol to obtain a concentrated solution; the concentration and ethanol recovery are carried out simultaneously. During the ethanol recovery process, the vacuum degree is -0.03 MPa and the temperature is 80 ± 5 °C; during the concentration process, the vacuum degree is -0.05 MPa and the temperature is 70 °C ± 5 °C; the relative density of the concentrated solution at 60 °C is 1.16 - 1.18;

[0030] (5) Heat 12 - 18 parts by mass of purified water to boiling, add 35 - 45 parts by mass of a flavoring agent, continuously heat and stir until the flavoring agent is completely dissolved, and boil for 30 minutes to obtain a flavoring agent slurry;

[0031] (6) Mix the Ganoderma lucidum concentrated solution with the flavoring agent slurry, cool to 60 - 70 °C, add sodium benzoate and water, and mix evenly to obtain a premixed solution; then let the premixed solution stand and filter it with a 400 - mesh filter cloth to obtain the Ganoderma lucidum syrup.

[0032] In a specific embodiment of the present invention, the flavoring agent in step (5) is any one of xylitol, oligosaccharide, stevioside, and Momordica grosvenori extract.

[0033] In a specific embodiment of the present invention, the mass concentration of sodium benzoate in the premixed solution in step (6) is 0.2 - 0.3%, and the mass concentration of the flavoring agent is 45% - 60%. Among them, sodium benzoate is first dissolved with purified water.

[0034] The following further illustrates the present invention with reference to embodiments. In a specific embodiment of the present invention, the parts and proportions of the raw materials, without special instructions, are all parts by mass or mass ratios; the concentrations of the raw materials, without special instructions, are all mass concentrations.

[0035] Example 1

[0036] A preparation method of a sugar - free Ganoderma lucidum syrup for treating neurasthenia and insomnia is as follows:

[0037] (1) Crush Ganoderma lucidum to obtain Ganoderma lucidum coarse powder with a particle size of 400 μm, add an ethanol solution with a mass concentration of 75% to submerge the Ganoderma lucidum coarse powder, moisten and seal it for 1 hour to make it fully expand; evenly load the moist and expanded Ganoderma lucidum coarse powder into a percolation cylinder, apply appropriate pressure to make the Ganoderma lucidum coarse powder loose and uniform, the addition amount of the Ganoderma lucidum coarse powder does not exceed 2 / 3 of the percolation, and add an ethanol solution 10 cm higher than the Ganoderma lucidum coarse powder, cover the percolation cylinder, and impregnate for 7 days;

[0038] (2) After impregnation, percolation is carried out. When the percolate becomes colorless, the percolation ends. The percolate and Ganoderma lucidum medicinal residues are collected. During the percolation process, ethanol must always be kept 10 cm higher than the medicinal materials to prevent the medicinal materials from drying and cracking. Ethanol should be replenished into the percolation cylinder in a timely manner, and the percolation rate should be checked regularly. The flow rate of the percolate is 3 mL / min;

[0039] (3) Add water three times the weight of the Ganoderma lucidum coarse powder to the Ganoderma lucidum medicinal residues, heat to boiling and boil for 2 hours, and collect the medicinal liquid. Then add water twice the weight of the Ganoderma lucidum coarse powder to the medicinal residues again, heat to boiling and boil for 2 hours and collect the medicinal liquid. The two medicinal liquids are combined to obtain the water extract of Ganoderma lucidum;

[0040] (4) Combine the water extract of Ganoderma lucidum in step (3) with the percolate in step (2) to obtain the Ganoderma lucidum extract, and then concentrate the Ganoderma lucidum extract and recover ethanol to obtain the concentrated liquid. The concentration and ethanol recovery are carried out simultaneously. During the ethanol recovery process, the vacuum degree is -0.03 MPa and the temperature is 75 °C; during the concentration process, the vacuum degree is -0.05 MPa and the temperature is 65 °C; the relative density of the concentrated liquid at 60 °C is 1.17;

[0041] (5) Heat 18 parts by mass of purified water to boiling, add 45 parts by mass of a flavoring agent (xylitol), continuously heat and stir until the flavoring agent is completely dissolved, and boil for 30 minutes to obtain the flavoring agent slurry;

[0042] (6) Mix the Ganoderma lucidum concentrated liquid with the flavoring agent slurry, cool to 65 °C, add sodium benzoate and water, and mix evenly to obtain the premixed liquid. Then let the premixed liquid stand still and filter it with a 400-mesh filter cloth to obtain the Ganoderma lucidum syrup; the concentration of sodium benzoate in the premixed liquid is 0.3%, and the mass concentration of the flavoring agent is 50%; among them, sodium benzoate is first dissolved with purified water.

[0043] Example 2

[0044] A preparation method of a sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia is as follows:

[0045] (1) Crush Ganoderma lucidum to obtain Ganoderma lucidum coarse powder with a particle size of 500 μm, add an ethanol solution with a mass concentration of 85% to submerge the Ganoderma lucidum coarse powder, moisten and seal it for 1 hour to make it fully expand. Evenly load the moist and expanded Ganoderma lucidum coarse powder into the percolation cylinder, apply appropriate pressure to make the Ganoderma lucidum coarse powder loose and uniform. The addition amount of the Ganoderma lucidum coarse powder does not exceed 2 / 3 of the percolation, and add an ethanol solution 7 cm higher than the Ganoderma lucidum coarse powder, cover the percolation cylinder, and impregnate for 7 days;

[0046] (2) After impregnation, percolation is carried out. When the percolate becomes colorless, the percolation ends, and the percolate and Ganoderma lucidum medicinal residues are collected. During the percolation process, ethanol must always be kept 7 cm higher than the medicinal materials to prevent the medicinal materials from drying and cracking. Ethanol should be replenished into the percolation cylinder in a timely manner, and the percolation rate should be checked regularly. The flow rate of the percolate is 5 mL / min;

[0047] (3) Add water that is 5 times the weight of the Ganoderma lucidum coarse powder to the above-mentioned Ganoderma lucidum medicinal residues, heat to boiling and boil for 2 hours, and collect the medicinal liquid; then add water that is 3 times the weight of the Ganoderma lucidum coarse powder to the medicinal residues again, heat to boiling and boil for 2 hours and collect the medicinal liquid; combine the two medicinal liquids to obtain the water extract of Ganoderma lucidum;

[0048] (4) Combine the water extract of Ganoderma lucidum in step (3) with the percolate in step (2) to obtain the Ganoderma lucidum extract, and then concentrate the Ganoderma lucidum extract and recover ethanol to obtain the concentrated liquid; the concentration and ethanol recovery are carried out simultaneously. During the ethanol recovery process, the vacuum degree is -0.03 MPa and the temperature is 80 °C; during the concentration process, the vacuum degree is -0.05 MPa and the temperature is 70 °C; the relative density of the concentrated liquid at 60 °C is 1.18;

[0049] (5) Heat 18 parts by mass of purified water to boiling, add 45 parts of flavoring agent (stevioside) to it, continuously heat and stir until the flavoring agent is completely dissolved, and boil for 30 minutes to obtain the flavoring agent slurry;

[0050] (6) Mix the Ganoderma lucidum concentrated liquid with the flavoring agent slurry, cool to 65 °C, add sodium benzoate and water, and mix evenly to obtain the premixed liquid; then let the premixed liquid stand still, filter it with a 400-mesh filter cloth to obtain the Ganoderma lucidum syrup. The mass concentration of sodium benzoate in the premixed liquid is 0.2%, and the mass concentration of the flavoring agent is 60%; among them, sodium benzoate is first dissolved with purified water.

[0051] Example 3

[0052] A preparation method of a Ganoderma lucidum plant beverage containing Ganoderma lucidum syrup, the steps are as follows:

[0053] (1) Weigh the raw medicinal materials according to the mass ratio of Angelica sinensis, Schisandra chinensis, Ziziphus jujuba var. spinosa, Glycyrrhiza uralensis, Lilium brownii, Poria cocos, and Lycium barbarum being 2:2.5:4:3:3.5:4:2, wash and dry them; add the above-mentioned medicinal materials to 3 times the mass of water and soak for 25 h, then heat and decoct for 2 h, and filter; add the filter residue obtained by filtration to 3 times the mass of water again and decoct for 1 h, and filter; combine the two filtrates; add the filtrate to ethanol and let it stand for 3 h, filter, recover ethanol under reduced pressure, and concentrate the filtrate to obtain a medicinal extract with a relative density of 1.25; the volume ratio of the filtrate to ethanol is 1:2;

[0054] (2) Mix the medicinal extract with the Ganoderma lucidum concentrated liquid prepared in Example 1 at a volume ratio of 3:1 to obtain the sugar-free Ganoderma lucidum plant beverage for improving neurasthenia and insomnia.

[0055] Example 4

[0056] A preparation method of a Ganoderma lucidum plant beverage containing Ganoderma lucidum syrup is as follows:

[0057] (1) Weigh the raw medicinal materials of Angelica sinensis, Schisandra chinensis, Ziziphus jujuba var. spinosa, Glycyrrhiza uralensis, Lilium brownii, Poria cocos, and Lycium barbarum according to a mass ratio of 2:3:5:1:3.4:4, wash and air-dry them; add the above traditional Chinese medicines to 3 times the mass of water and soak for 30 h, then heat and decoct for 1.5 h, and filter; add the filter residue obtained by filtration to 3 times the mass of water again and decoct for 1 h, and filter; combine the two filtrates; add the filtrate to ethanol and let it stand for 3 h, filter, recover ethanol under reduced pressure, and concentrate the filtrate to obtain a medicinal extract with a relative density of 1.25; the volume ratio of the filtrate to ethanol is 1:2;

[0058] (2) Mix the medicinal extract with the Ganoderma lucidum concentrated liquid prepared in Example 1 at a volume ratio of 2:1 to obtain the sugar-free Ganoderma lucidum plant beverage for improving neurasthenia and insomnia.

[0059] Comparative Example 1

[0060] A preparation method of Ganoderma lucidum syrup refers to the method of Example 1, except that xylitol in Example 1 is replaced by sucrose.

[0061] Comparative Example 2

[0062] A preparation method of a sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia is as follows:

[0063] (1) Crush 200 g of Ganoderma lucidum to obtain Ganoderma lucidum coarse powder with a particle size of 1.0 mm, slowly percolate it with 5 times the amount of ethanol with a mass concentration of 80% until it becomes colorless, collect the percolate, combine the percolate with the impregnation liquid in step (1) to obtain a mixed liquid, and recover ethanol until the ethanol mass concentration is 37%; during the percolation process, the ethanol is 7 cm higher than the drug surface, and the flow rate of the percolate is 3 mL / min;

[0064] The remaining steps are the same as steps (3) to (6) of Example 1.

[0065] Seal the Ganoderma lucidum syrups prepared in Examples 1 to 2 and Comparative Examples 1 to 2, and place them under light-shielded conditions below 30 °C for 180 days, and measure their relative densities at 0 day, 30 days, 60 days, 90 days, and 180 days. The results are shown in Table 1.

[0066] Table 1

[0067]

[0068] It can be seen from Table 1 that the Ganoderma lucidum syrup prepared by the present invention has good stability.

[0069] The Ganoderma lucidum syrup prepared in Example 1 and Comparative Examples 1-2 was used to conduct an experiment on the treatment of insomnia in mice, and the steps were as follows:

[0070] (1) Establishment of insomnia model: 72 SPF healthy male Kunming mice (5-7 weeks old, weighing (30±2) g) were randomly divided into 6 groups (model group, blank control group, positive control group, and treatment groups 1-3). After 7 days of normal feeding and adaptation to the environment, the model group, positive control group, and treatment group were intraperitoneally injected with 400 mg / kg PCPA suspension (PCPA suspension is prepared by adding powder and water to 60 mg / mL) at a fixed time every day for 2 consecutive days, and the blank control group was injected with an equal volume of normal saline. The status of each group was observed after 2 days. Except for the blank control group, the mice showed circadian disorder and mutual aggression, indicating that the insomnia model was successfully established.

[0071] (2) After the modeling was completed, each group was medicated. The blank control group and the model group were gavaged with normal saline for 7 consecutive days; the positive control group was gavaged with melatonin (10 mg / kg) for 7 consecutive days; and the treatment groups 1 to 3 were gavaged with the Ganoderma lucidum syrup (2 mg / kg) prepared in Example 1 and Comparative Examples 1 to 2 for 7 consecutive days.

[0072] The body weight of mice after modeling and the last administration was recorded, and the average increase rate was calculated. One hour after the last oral administration, the mice were placed in the spontaneous activity instrument for 3 minutes to adapt, and the number of spontaneous activities and sleep time were measured. The results are shown in Table 2.

[0073] Table 2

[0074] Experimental group Gavage drug Body weight change (%) Spontaneous activity times Sleep time (min) Model group Normal saline -0.62 172±33 43±12 Blank control group Normal saline 4.15 124±24 57±14 Positive control group Melatonin 5.13 102±27 80±17 Treatment group 1 Example 1 5.17 89±25 82±15 Treatment group 2 Comparative example 1 5.13 86±25 85±20 Treatment group 3 Comparative example 2 3.67 103±30 70±21

[0075] It can be seen from Table 2 that the Ganoderma lucidum syrup prepared by the present invention can significantly improve the insomnia symptoms of mice and has a significant sedative and tranquilizing effect.

[0076] The Ganoderma lucidum syrup of Example 1 and Comparative Examples 1-2 was studied for clinical application. Basic case situation: 120 cases of insomnia were clinically treated, including 60 males and 60 females, aged between 22 and 55. The clinical manifestations were: tossing and turning when falling asleep, difficulty falling asleep or dreaming and easily startled, emotional discomfort, depression, closed ears, difficulty falling asleep, shallow sleep, easy awakening, dreaming, waking up early, and difficulty falling asleep again after waking up. The above patients were randomly divided into 3 groups, and there was no significant difference in age, gender, and clinical symptoms among the groups. The patients took the Ganoderma lucidum syrup of Example 1 and Comparative Examples 1-2, 3 times a day, 20 mL each time, for 14 consecutive days.

[0077] Judging criteria:

[0078] Recovery: Can fall asleep within half an hour, with a comfortable mood and full of energy;

[0079] Effective: The sleep disorder and other symptoms are significantly improved, not easy to wake up after sleeping, with a comfortable mood and in great spirits;

[0080] Ineffective: After treatment, there is almost no change in symptoms and signs compared with those before treatment.

[0081] The results are shown in Table 3.

[0082] Table 3

[0083] Drug Number of people Cured Effective Invalid Total effective rate (%) Cure rate (%) Example 1 40 36 4 0 100 90 Comparative example 1 40 35 5 0 100 87.5 Comparative example 2 40 30 2 8 80 75

[0084] It can be seen from Table 3 that the Ganoderma lucidum syrup prepared in this application can significantly improve the insomnia symptoms of patients, and has a significant effect of calming the nerves.

[0085] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can still be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A method for preparing a sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia, characterized in that: The following steps are involved: (1) Ganoderma lucidum is crushed to obtain coarse powder, ethanol solution is added to immerse the coarse powder, and the coarse powder is placed in a wet and sealed container for more than 1 hour to allow it to fully swell; the coarse powder after swollen and wet is evenly loaded into a percolation cylinder, and ethanol solution is added to a level 5-10 cm higher than the coarse powder, the cylinder is covered, and the percolation cylinder is immersed for one week; (2) After the soaking is completed, percolation is performed. When the percolation liquid becomes colorless, the percolation is completed and the percolation liquid and the Ganoderma lucidum residue are collected; (3) adding water to the Ganoderma lucidum residue, heating and boiling for 2 hours, and collecting the liquid; then adding water to the residue again, heating and boiling for 2 hours and collecting the liquid; combining the two liquids to obtain a Ganoderma lucidum water extract; (4) combining the Ganoderma lucidum aqueous extract in step (3) with the percolate in step (2) to obtain a Ganoderma lucidum extract, and then concentrating the Ganoderma lucidum extract and recovering ethanol to obtain a concentrated solution; (5) Heat purified water to boiling, add flavoring agent thereto, continue heating and stirring to completely dissolve the flavoring agent, boil for 20 to 30 minutes to obtain flavoring agent slurry, and cool to below 70°C; (6) mixing the Ganoderma lucidum concentrate with the flavoring agent slurry, cooling the mixture to 60-70° C., adding sodium benzoate and water, and mixing the mixture to obtain a premixed solution; then allowing the premixed solution to stand, and filtering the mixture with a 400-mesh filter cloth to obtain the Ganoderma lucidum syrup; The flavoring agent in step (5) is xylitol or stevia; The mass ratio of the purified water to the flavoring agent in step (5) is 12-18:35-45; The particle size of the coarse Ganoderma lucidum powder in step (1) is 50-500 μm.

2. The method for preparing the sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia according to claim 1, characterized in that: The mass concentration of the ethanol is 80±5%.

3. The method for preparing the sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia according to claim 1, characterized in that: During the percolation process in step (2), the ethanol is 5-10 cm above the drug surface, and the flow rate of the percolation liquid is 3-5 mL / min.

4. The method for preparing the sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia according to claim 1, characterized in that: The amount of water added in the first step (3) is 3-5 times the weight of the coarse powder of Ganoderma lucidum, and the amount of water added in the second step is 2-3 times the weight of the coarse powder of Ganoderma lucidum.

5. The method for preparing the sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia according to claim 1, characterized in that: In the ethanol recovery process of step (4), the vacuum degree is -0.03 MPa and the temperature is 80±5°C; in the concentration process, the vacuum degree is -0.05 MPa and the temperature is 70°C±5°C.

6. The method for preparing the sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia according to claim 1, characterized in that: The relative density of the concentrated solution in step (4) at 60° C. is 1.16 to 1.

18.

7. The method for preparing the sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia according to claim 1, characterized in that: The mass concentration of sodium benzoate in the premixed solution of step (6) is 0.2-0.3%, and the mass concentration of the flavoring agent is 45%-60%.

8. A sugar-free Ganoderma lucidum syrup for treating neurasthenia and insomnia prepared according to the preparation method according to any one of claims 1 to 7.

Images