Soothing anti-inflammatory traditional Chinese medicine composition, facial mask liquid and preparation method of facial mask liquid

Abstract

The invention discloses a soothing and anti-inflammatory traditional Chinese medicine composition which is prepared from the following raw materials in parts by weight: 8-13 parts of honeysuckle, 3-8 parts of oldenlandia diffusa, 8-13 parts of radix rehmanniae recen, 25-35 parts of purslane, 13-18 parts of fructus forsythiae and 8-13 parts of centella asiatica. According to the present invention, the soothing component can effectively relieve skin sensitivity, redness and swelling and other problems, can help to soothe skin, reduce skin allergy, reduce skin pressure, make skin healthier, effectively supplement water, make skin more moist, make skin recover elasticity, relieve skin aging phenomenon, improve the overall condition of skin, and make skin more comfortable. The discomfort of the skin is relieved, and the skin becomes more tender, smoother and softer.

Description

Technical Field

[0001] The invention relates to a soothing and anti-inflammatory Chinese medicine composition, a facial mask liquid and a preparation method thereof, and relates to A61K, and in particular to the field of dressing preparations. Background Art

[0002] With the development of society and the advancement of science and technology, people are under increasing pressure in their lives. The reversed day and night routines have caused people's skin conditions to become worse and worse, and allergies, redness, swelling and other problems have appeared more and more frequently. Therefore, it is necessary to develop products with soothing, anti-inflammatory and sedative effects to achieve the technical effect of maintaining skin stability. The use of natural Chinese medicine components is safer on the surface of the skin. However, there is a problem of incomplete extraction of Chinese medicine components, which requires a large amount of Chinese medicinal materials to extract, and the recovery rate of the paste is low. The content of chlorogenic acid in the extract is not high. After optimizing the process, efficient extraction of effective substances can be achieved. In addition, it is also crucial to develop a mask liquid that can stably apply high-content chlorogenic acid extracts.

[0003] Chinese invention patent CN202010496089.0 discloses a composition for removing acne, eliminating sores and repairing acne marks and its preparation method, which uses a combination of extracts of medicinal materials such as honeysuckle, purslane, and Centella asiatica to achieve the effects of removing acne, reducing inflammation and repairing, but the direct use of extracts has a high preparation cost, and relying solely on the combination of raw materials cannot achieve stable storage of drugs, and the efficacy of long-term placement of Chinese medicine compositions will significantly decrease. Chinese invention patent CN202011539786.6 discloses a plant fermented allergy-relieving composition and its preparation method and application, which uses chamomile, Centella asiatica, peony, Panax notoginseng, purslane, etc. as substrates to ferment into an allergy-relieving composition, which has the effects of relieving skin allergies, antibacterial and anti-inflammatory, but the fermentation time is long and the preparation cost is high. Summary of the invention

[0004] In order to improve the extraction rate of effective ingredients in traditional Chinese medicine extracts and ensure the stable application of extracts with high chlorogenic acid content, the first aspect of the present invention provides a soothing and anti-inflammatory traditional Chinese medicine composition, the preparation raw materials include, by weight: 8-13 parts of honeysuckle, 3-8 parts of oldenlandia diffusa, 8-13 parts of rehmannia root, 25-35 parts of purslane, 13-18 parts of forsythia, and 8-13 parts of Centella asiatica.

[0005] As a preferred embodiment, the preparation raw materials include, by weight: 9-11 parts of honeysuckle, 4-6 parts of oldenlandia diffusa, 9-11 parts of rehmannia root, 29-32 parts of purslane, 14-16 parts of forsythia, and 9-11 parts of centella asiatica.

[0006] As a preferred embodiment, the preparation raw materials include, by weight: 10 parts of honeysuckle, 5 parts of oldenlandia diffusa, 10 parts of rehmannia root, 30 parts of purslane, 15 parts of forsythia, and 10 parts of centella asiatica.

[0007] As a preferred embodiment, the method for preparing the soothing and anti-inflammatory Chinese medicine composition comprises the following steps:

[0008] S1. Combining the raw materials for preparing the soothing and anti-inflammatory Chinese medicine composition into a Chinese medicine compound according to weight portions;

[0009] S2 is extracted by adding water, filtered, and an extract is obtained;

[0010] S3: subjecting the extract to alcohol precipitation and activated carbon decolorization treatment to obtain a Chinese medicine composition.

[0011] As a preferred embodiment, the weight ratio of water to the traditional Chinese medicine composition in step S2 of the preparation method is (8-12):1.

[0012] As a preferred embodiment, the weight ratio of water to the traditional Chinese medicine composition in step S2 of the preparation method is 12:1.

[0013] As a preferred embodiment, the extraction times of step S2 in the preparation method are 2-5 times, and the single extraction time is 1-2 hours.

[0014] As a preferred embodiment, the extraction times of step S2 in the preparation method is 3 times, and the single extraction time is 1 hour.

[0015] The applicant found in the experiment that the extract obtained by using 12 times the amount of water and extracting three times for 1 hour each time had the highest chlorogenic acid content and the best paste yield. It is speculated that the possible reason is that using 12 times the amount of water to extract the Chinese medicine composition and extracting it three times can gradually extract the Chinese medicine combination, improve the extraction efficiency of the Chinese medicine combination, and thus obtain a higher chlorogenic acid extraction efficiency.

[0016] The second aspect of the present invention provides a facial mask liquid, the raw materials for preparing the facial mask liquid include a soothing and anti-inflammatory Chinese medicine composition.

[0017] As a preferred embodiment, the preparation raw materials include, by weight percentage, phase A: thickener 0.1-0.5%, humectant I 0.1-1%, humectant II 5-10%, purified water I supplemented to 100%; phase B: Chinese medicine composition 1-15%, humectant III 0.5-1%, humectant IV 0.5-1%, purified water II 10-20%; phase C: humectant V 0.1-1%, preservative 0.1-5%, humectant VI 0.1-1%, humectant VII 0.5-2%, purified water III 10-20%.

[0018] As a preferred embodiment, the preparation raw materials include, by weight percentage, phase A: thickener 0.2%, humectant I 0.1%, humectant II 5%, purified water I supplemented to 100%; phase B: Chinese medicine composition 10%, humectant III 1%, humectant IV 1%, purified water II 15%; phase C: humectant V 0.2%, preservative 0.5%, humectant VI 0.5%, humectant VII 2%, purified water III 15%.

[0019] As a preferred embodiment, the moisturizing agents I-VII are selected from one or a combination of hyaluronic acid, glycerin, betaine, urea, allantoin, polyols, sodium lactate, pyrrolidone carboxylic acid, ceramide, and panthenol.

[0020] As a preferred embodiment, the polyol is selected from one or a combination of butanediol, propylene glycol, hexylene glycol, polypropylene glycol, sorbitol, and polyethylene glycol.

[0021] As a preferred embodiment, the moisturizer I is hyaluronic acid, the moisturizer II is glycerol, the moisturizer III is betaine, the moisturizer IV is urea, the moisturizer V is allantoin, the moisturizer VI is 1,2-hexanediol, and the moisturizer VII is butylene glycol.

[0022] As a preferred embodiment, the thickener is selected from one or a combination of xanthan gum, hydroxyethyl cellulose, acrylic acid copolymer, and carbomer.

[0023] As a preferred embodiment, the acrylic copolymer is selected from one or a combination of ammonium acryloyldimethyltaurate / VP copolymer, polyacrylate cross-linked polymer-6, and acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer.

[0024] The third aspect of the present invention provides a method for preparing a facial mask liquid, comprising the following steps:

[0025] M1 weighs the thickener and humectant I of phase A, adds humectant II and disperses and stirs evenly, then adds purified water I and mixes and stirs evenly to make it fully swell to obtain phase A;

[0026] M2 weighs the materials of phase B, mixes and stirs evenly to obtain phase B;

[0027] M3 weighs the C phase material and heats it in a water bath at 45-55°C to completely dissolve it to obtain the C phase;

[0028] Slowly pour phase B and phase C into phase A while stirring with M4, stir evenly to make the materials disperse completely, and obtain the facial mask liquid.

[0029] The applicant further found that the chlorogenic acid content in the obtained extract is high. During the application process, the stability is poor, it is easy to oxidize, the color deepens, and the composition state changes in different thicknesses, resulting in a decrease in the effect of the product. The present application uses xanthan gum as a thickener, which can further improve the stability of the Chinese medicine combination extract in the product and improve the stability of the product. The possible reason is that the combination of xanthan gum, p-hydroxyacetophenone, and glycerol can maintain the uniform dispersion of the Chinese medicine composition in the product, and maintain the stability of the product state, maintain the consistency of the thickness, maintain the consistency of the upper and lower states during long-term storage, and keep the color and smell stable at high temperature and high and low temperature cycle tests. If other thickeners are used, the state of the product will change, and the problem of inconsistent thickness will occur, affecting the stability of the product.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] (1) The soothing and anti-inflammatory Chinese medicine composition of the present invention uses 12 times the amount of water and is extracted three times, each time for 1 hour. The extract obtained has a high content of chlorogenic acid and an optimal paste yield. In addition, the soothing and anti-inflammatory Chinese medicine composition of the present invention has a lower color and a higher purity.

[0032] (2) The soothing and anti-inflammatory Chinese medicine composition of the present invention can improve the stability of the combined extract in the facial mask liquid by combining the Chinese medicine combination extract with a moisturizer and a thickener, thereby achieving excellent moisturizing, anti-allergic, soothing, repairing and anti-inflammatory effects.

[0033] (3) The soothing and anti-inflammatory Chinese medicine composition of the present invention uses xanthan gum as a thickener and the Chinese medicine extract is used in an amount of 10%, which can improve the storage stability of the mask liquid and can be stored for a long time in an environment of room temperature, light, high temperature, refrigeration, freezing, and cold and hot cycles.

[0034] (4) The facial mask liquid prepared from the soothing and anti-inflammatory Chinese medicine composition of the present invention has soothing ingredients that can effectively relieve skin sensitivity, redness and swelling, help soothe the skin, reduce the occurrence of skin allergies, and reduce skin stress, making the skin healthier. It can effectively replenish water, make the skin more moisturized, restore skin elasticity, relieve skin aging, and improve the overall condition of the skin, relieve skin discomfort, and make the skin more tender, smooth and soft. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 The expression levels of mRNA of inflammatory related factors in RAW264.7 cells after 24 hours of treatment with different groups.

[0036] Figure 2 The growth of the tail fin of zebrafish cells treated with different groups for 24 hours. DETAILED DESCRIPTION

[0037] Example 1

[0038] A soothing and anti-inflammatory Chinese medicine composition. The raw materials for its preparation include, by weight, 10 parts of honeysuckle, 5 parts of oldenlandia diffusa, 10 parts of rehmannia root, 30 parts of purslane, 15 parts of forsythia, and 10 parts of centella asiatica.

[0039] The preparation method of the soothing and anti-inflammatory Chinese medicine composition comprises the following steps:

[0040] S1. Combining the raw materials for preparing the soothing and anti-inflammatory Chinese medicine composition into a Chinese medicine compound according to weight portions;

[0041] S2 is extracted by adding water, filtered, and an extract is obtained;

[0042] S3: subjecting the extract to alcohol precipitation and activated carbon decolorization treatment to obtain a Chinese medicine composition, and drying the Chinese medicine composition to obtain a dry paste.

[0043] The weight ratio of water to the traditional Chinese medicine composition in step S2 of the preparation method is 12:1.

[0044] The extraction times of step S2 in the preparation method are 3 times, and the single extraction time is 1 hour.

[0045] Example 2

[0046] A soothing and anti-inflammatory Chinese medicine composition, the specific implementation method is the same as that of Example 1, except that the weight ratio of water to the Chinese medicine composition in step S2 of the preparation method is 10:1.

[0047] The extraction times of step S2 in the preparation method are 3 times, and the single extraction time is 2 hours.

[0048] Example 3

[0049] A soothing and anti-inflammatory Chinese medicine composition, the specific implementation method is the same as that of Example 1, except that the weight ratio of water to the Chinese medicine composition in step S2 of the preparation method is 12:1.

[0050] The extraction times in step S2 of the preparation method are 1, and the single extraction time is 2 hours.

[0051] Example 4

[0052] A facial mask liquid, the preparation raw materials of which include, by weight percentage, phase A: 0.2% thickener, 0.1% moisturizer I, 5% moisturizer II, and purified water I supplemented to 100%; phase B: 10% Chinese medicine composition, 1% moisturizer III, 1% moisturizer IV, and 15% purified water II; phase C: 0.2% moisturizer V, 0.5% preservative, 0.5% moisturizer VI, 2% moisturizer VII, and 15% purified water III.

[0053] The moisturizer I is hyaluronic acid, the moisturizer II is glycerol, the moisturizer III is betaine, the moisturizer IV is urea, the moisturizer V is allantoin, the moisturizer VI is 1,2-hexanediol, and the moisturizer VII is butanediol.

[0054] The thickener is xanthan gum, and the traditional Chinese medicine composition is the traditional Chinese medicine composition prepared in Example 1.

[0055] The preservative is p-hydroxyacetophenone.

[0056] A method for preparing a facial mask liquid comprises the following steps:

[0057] M1 weighs the thickener and humectant I of phase A, adds humectant II and disperses and stirs evenly, then adds purified water I and mixes and stirs evenly to make it fully swell to obtain phase A;

[0058] M2 weighs the materials of phase B, mixes and stirs evenly to obtain phase B;

[0059] M3 weighs the C phase material and heats it in a 50°C water bath to completely dissolve it to obtain the C phase;

[0060] Slowly pour phase B and phase C into phase A while stirring with M4, stir evenly to make the materials disperse completely, and obtain the facial mask liquid.

[0061] Example 5

[0062] A facial mask liquid, the specific implementation method is the same as that of Example 4, except that the thickener is hydroxyethyl cellulose purchased from Ashland (China) Investment Co., Ltd.

[0063] Example 6

[0064] A facial mask liquid, the specific implementation method is the same as Example 4, except that the thickener is ammonium acryloyldimethyltaurate / VP copolymer purchased from Clariant Chemical Technology (Shanghai) Co., Ltd.

[0065] Example 7

[0066] A facial mask liquid, the specific implementation manner is the same as that of Example 4, except that the thickener is polyacrylate cross-linked polymer-6, purchased from SEPPIC SA (Seppic (Shanghai) Special Chemicals Co., Ltd.).

[0067] Example 8

[0068] A facial mask liquid, the specific implementation method is the same as that of Example 4, except that the thickener is acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer purchased from Lubrizol Advanced Materials, Inc.

[0069] Example 9

[0070] A facial mask liquid, the specific implementation manner is the same as that of Example 4, except that the thickener is Carbomer Ultrez 10.

[0071] Example 10

[0072] A facial mask liquid, the specific implementation method is the same as that of Example 4, except that the weight ratio of the traditional Chinese medicine composition is 5%.

[0073] Embodiment 11

[0074] A facial mask liquid, the specific implementation method is the same as that of Example 4, except that the weight ratio of the traditional Chinese medicine composition is 15%.

[0075] Example 12

[0076] A facial mask liquid, the specific implementation method is the same as that of Example 4, except that the weight ratio of the traditional Chinese medicine composition is 20%.

[0077] Performance Testing

[0078] 1. HPLC was used to test the chlorogenic acid content and cream yield in the traditional Chinese medicine compositions of Examples 1 to 3. The test results are shown in Table 1.

[0079] Table 1

[0080] Chlorogenic acid content mg / mL Cream recovery rate / % Example 1 3.78 35.6 Example 2 2.59 39.09 Example 3 1.76 17.31

[0081] 2. Soothing effect test

[0082] RAW264.7 cells were treated with lipopolysaccharide (LPS) and cultured at 10 6 cells / well were inoculated into cell culture dishes and exposed according to group requirements after 24 hours of culture. After 24 hours, the cells were washed with PBS 2-3 times, and total RNA was extracted. After reverse transcription, qPCR was performed to detect the mRNA expression of inflammation-related genes IL-1β, IL-8, and TNF-α. The test results are shown in Figure 1 , Figure 1 The sample in the middle is the extract of Example 1.

[0083] 3. Repair efficacy test

[0084] Zebrafish fry with normal morphology were picked after 72 hours of incubation and placed in a 6-well culture plate, 10 in each group, and repeated 3 times. The blank control group, positive control group and sample group all used surgical methods to quantitatively cut off the tail fin to complete the modeling. The blank group was not treated with drugs, the positive control group was added with 25μM / L Centella asiatica extract (5μg / mL dexamethasone), and the sample group was added with 0.05% / 0.1% sample. After incubating in the dark for a period of time, each group of zebrafish was photographed under a microscope and the pictures were saved. The image analysis software was used to perform quantitative statistical analysis on the zebrafish tail fin area. The test results are shown in Figure 2 .

[0085] 4. Anti-inflammatory efficacy test

[0086] Using human immortalized keratinocytes Hacat as a platform, a lipopolysaccharide (LPS)-induced Hacat cell inflammation model was established to study the anti-inflammatory activity of Chinese medicine extracts on Hacat cells. The results showed that Chinese medicine extracts of different concentrations (3%, 1.5%, 0.75%) had different degrees of inhibitory effects on the increase of LPS-induced cellular inflammatory factors IL-6, IL-1β, TNF-α, and PEG-2, and showed good dose dependence. Among them, the high-dose group of Chinese medicine extracts (3.0%) had better inhibition on the production of IL-6 and IL-1β than dipotassium glycyrrhizinate (5mg / ml), and the inhibition on the production of TNF-α and PEG-2 was equivalent to dipotassium glycyrrhizinate (5mg / ml). It shows that Chinese medicine extracts have a significant inhibitory effect on the increase of LPS-induced cellular inflammatory factor levels and show good anti-inflammatory activity. The test results are shown in Table 13.

[0087] 5. Stability test

[0088] The stability of the mask liquid thickness of Examples 4 to 9 was tested by sensory evaluation and viscosity test, and the test results are shown in Tables 2 to 8. The test standards are shown in Table 2.

[0089] Table 2

[0090]

[0091]

[0092] Table 3

[0093]

[0094] Table 4

[0095]

[0096]

[0097] Table 5

[0098]

[0099]

[0100] Table 6

[0101]

[0102] Table 7

[0103]

[0104]

[0105] Table 8

[0106]

[0107]

[0108] The results show that the viscosity and consistency of Examples 4 and 5 are basically unchanged, and the system is stable; the viscosity of Examples 6 and 7 is slightly reduced, and the system is relatively stable; the viscosity of Examples 8 and 9 is significantly reduced, indicating that the system is extremely unstable. However, the viscosity of Example 5 is relatively sticky, which brings discomfort to the skin, so the xanthan gum matrix of Example 4 is selected.

[0109] The sensory evaluation and viscosity test of Example 4 and Examples 10-12 were conducted under 5 conditions: room temperature, high temperature 40°C, refrigerated 4°C, frozen -10°C, and cycled -10°C / 40°C. The results of the first week, second week, third week, and fourth week were observed and recorded. The test results are shown in Tables 9-12.

[0110] Table 9

[0111]

[0112] Table 10

[0113]

[0114] Table 11

[0115]

[0116]

[0117] Table 12

[0118]

[0119] Table 13

[0120]

Claims

1. A soothing and anti-inflammatory Chinese medicine composition, characterized in that: The raw materials for preparation include, by weight: 8-13 parts of honeysuckle, 3-8 parts of oldenlandia diffusa, 8-13 parts of rehmannia root, 25-35 parts of purslane, 13-18 parts of forsythia, and 8-13 parts of centella asiatica.

2. The soothing and anti-inflammatory Chinese medicine composition according to claim 1, characterized in that: The preparation method of the soothing and anti-inflammatory Chinese medicine composition comprises the following steps: S1. Combining the raw materials for preparing the soothing and anti-inflammatory Chinese medicine composition into a Chinese medicine compound according to weight portions; S2 is extracted by adding water, filtered, and an extract is obtained; S3: subjecting the extract to alcohol precipitation and activated carbon decolorization treatment to obtain a Chinese medicine composition.

3. The soothing and anti-inflammatory Chinese medicine composition according to claim 2, characterized in that: The weight ratio of water to the traditional Chinese medicine composition in step S2 of the preparation method is (8-12):

1.

4. The soothing and anti-inflammatory Chinese medicine composition according to claim 2, characterized in that: The extraction times of step S2 in the preparation method are 2-5 times, and the single extraction time is 1-2 hours.

5. A facial mask liquid, characterized in that: The raw materials for its preparation include the soothing and anti-inflammatory Chinese medicine composition according to any one of claims 2 to 4.

6. The facial mask liquid according to claim 5, characterized in that: The raw materials for preparation include, by weight percentage, phase A: thickener 0.1-0.5%, moisturizer I 0.1-1%, moisturizer II 5-10%, purified water I supplemented to 100%; Phase B: 1-15% Chinese medicine composition, 0.5-1% moisturizer III, 0.5-1% moisturizer IV, 10-20% purified water II; Phase C: 0.1-1% moisturizer V, 0.1-5% preservative, 0.1-1% moisturizer VI, 0.5-2% moisturizer VII, 10-20% purified water III.

7. The facial mask liquid according to claim 6, characterized in that: The moisturizing agents I-VII are selected from one or a combination of hyaluronic acid, glycerin, betaine, urea, allantoin, polyol, sodium lactate, pyrrolidone carboxylic acid, ceramide, and panthenol.

8. The facial mask liquid according to claim 7, characterized in that: The polyol is selected from one or a combination of butanediol, propylene glycol, hexylene glycol, polypropylene glycol, sorbitol, and polyethylene glycol.

9. The facial mask liquid according to claim 6, characterized in that: The thickener is selected from one or a combination of xanthan gum, hydroxyethyl cellulose, acrylic acid copolymer and carbomer.

10. A method for preparing the facial mask liquid according to any one of claims 6 to 9, characterized in that: The following steps are involved: M1 weighs the thickener and humectant I of phase A, adds humectant II and disperses and stirs evenly, then adds purified water I and mixes and stirs evenly to make it fully swell to obtain phase A; M2 weighs the materials of phase B, mixes and stirs evenly to obtain phase B; M3 weighs the C phase material and heats it in a water bath at 45-55°C to completely dissolve it to obtain the C phase; Slowly pour phase B and phase C into phase A while stirring with M4, stir evenly to disperse the materials completely, and obtain the facial mask liquid.

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