Automatic PRP separator and extraction and separation method thereof

Abstract

The invention discloses an automatic PRP (platelet rich plasma) separator which comprises a sterile syringe for sampling, a centrifugal machine for providing a centrifugal effect, a fixing assembly matched with the centrifugal machine for use, and a separator for performing sample purification on the centrifuged sterile syringe, the fixing assembly comprises a detachable base, a force application mechanism arranged above the base and used for pushing and pulling a piston column of a sterile syringe, a support fixed to the base and used for fixing the sterile syringe to be centrifuged, and a color selector used for conducting dynamic color selection on an injection opening of the sterile syringe. And the base is fixed through a fixing seat arranged on the rotating disc. According to the automatic PRP separator and the extraction and separation method thereof provided by the invention, a sample does not need to be taken and placed for many times, and sampling is carried out by adopting automatic identification of a machine, so that the problem of contact with the outside in the transferring and centrifuging processes is effectively solved, the risk of pollution in the PRP preparation process is reduced, and meanwhile, extraction is convenient, the purity is improved, and residual pollution is rejected.

Description

Technical Field

[0001] The present invention belongs to the field of medical devices, and in particular relates to a PRP preparation and extraction device and method, specifically a PRP automatic separator and an extraction and separation method thereof. Background Art

[0002] Platelet-rich plasma (PRP) is an extract of autologous whole blood that contains high concentrations of platelets and growth factors. Studies in recent years have shown that the concentrations of interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) in PRP are closely related to its leukocyte concentration. The results suggest that L-PRP has a strong anti-inflammatory effect. At the same time, high concentrations of leukocytes may have an inhibitory effect on tissue repair. P-PRP has received more and more attention in recent years, and the methods for preparing P-PRP are also increasing. The existing P-PRP preparation methods can be roughly divided into two categories, including blood filtration and centrifugation. Among them, the latter has the advantages of simple operation, no need for special equipment and consumables, and low cost, and is more suitable for clinical use.

[0003] PRP therapy has the characteristics of being derived from the body, having no immune rejection, and being relatively simple to make, etc. Therefore, it is widely used in orthopedics, rehabilitation, burns, dentistry, maxillofacial surgery, sports medicine, and cosmetology.

[0004] Currently, PRP sampling mainly uses sterile syringes for sampling. The blood samples are centrifuged to produce layers, from top to bottom:

[0005] 1. Plasma layer: generally the top layer, usually a light yellow transparent liquid, containing water, plasma globulin, serum protein, inorganic salts and other substances;

[0006] 2. Leukocyte and platelet layer: It is the middle layer, usually a thin white layer, which mainly contains leukocytes such as neutrophils, basophils, monocytes, etc. In addition, it also contains platelets;

[0007] 3. Red blood cell layer: It is the bottom layer, mainly composed of red blood cells, dark red in color, and an opaque solid.

[0008] The plasma layer accounts for about 44% of the total volume, and the middle layer accounts for about 1% of the total volume. It is pure PRP, but due to its relatively small number, it is easy to mix with the upper and lower layers, red blood cells (accounting for about 55% of the total volume); the extraction of PRP is the focus of discussion in this invention. In actual use, PRP contains part of the plasma layer.

[0009] However, at present, most of the domestic and foreign methods use open manual secondary centrifugation to prepare PRP (see Figure 1 The existing PRP preparation methods have the following defects: (1) the open preparation system is easily contaminated by the outside world; (2) the separation reagent used in blood cell separation is prone to residues of the separation reagent; (3) the preparation of PRP with higher purity by multiple centrifugation requires multiple loading and unloading, which increases the possibility of secondary contamination; (4) manual visual judgment and extraction of useful substances in PRP require high technical requirements of the operator, which is prone to errors and results in differences in the use effect.

[0010] During the research and development of the present invention, according to actual needs, some existing documents were referenced, and some of the referenced patent documents are as follows:

[0011] 1. CN2021112675686-A centrifugal system for preparing leukocyte-depleted platelet-rich plasma and its production method; 2. CN2021209214406-A closed centrifugal sleeve device for preparing PRP; CN2021226175525-A centrifuge for preparing PRP; 3. CN202010262345X-PRP centrifugal sleeve, PRP centrifuge and PRP preparation method; 4. CN2019106081293-Digital limb tissue flap blood circulation observer; 5. CN2013100272853-A colorimeter for measuring the color of an object using a linear variable filter; 6. CN2013101341494-A device for extracorporeal blood treatment with a leakage sensor; 7. CN2014102293384-A non-destructive blood glucose detector and detection method based on Raman scattering of blood in ocular vessels.

[0012] The above patent technologies do not constitute technical disclosure or technical inspiration for the technology of the present invention. Summary of the invention

[0013] The purpose of the present invention is to address the above problems and provide a PRP automatic separator and an extraction and separation method thereof, which does not require multiple sample taking and placing, and the sampling is carried out by automatic machine recognition, which effectively solves the problem of contact with the outside world during transfer and centrifugation, reduces the risk of contamination during the PRP preparation process, and facilitates extraction, improves purity, and rejects residual contamination.

[0014] To achieve the above objectives, the technical solution adopted by the present invention is: a PRP automatic separator, including a sterile syringe for sampling, a centrifuge providing a centrifugal effect, a fixing component used in conjunction with the centrifuge, and a separator for purifying the sample of the sterile syringe after centrifugation; the fixing component includes: a detachable base, and a force-applying mechanism arranged above the base for pushing and pulling the piston column of the sterile syringe, a bracket fixed on the base for fixing the sterile syringe to be centrifuged, and a color sorter for dynamically color sorting the injection port of the sterile syringe; the base is fixed by a fixing seat arranged on a rotating disk; the separator is a three-way pipe, one end of which is provided with an end seal when in use, and the other two ends are respectively connected to the sterile syringe after centrifugation and the sterile syringe for extracting operation; the bottom of the base of the base is provided with a power plug and a signal connector, and it corresponds to the socket arranged on the rotating disk; the separator is provided with a control circuit board CPU, a centrifuge, and a mains interface, and the circuit board CPU is electrically connected to the centrifuge, the base, and the force-applying mechanism and the color sorter on the base.

[0015] Furthermore, the sterile syringes are three syringes with threaded injection ports of the same model, which are connected in series in parallel through three separators; the one at the right end is a sampling sterile syringe, and the other two are blank syringes, and the original state of the blank syringes is that the piston column is at the lowest position.

[0016] Furthermore, the fixed component is a C-shaped structure, and the fixed component includes an external base and a bracket rotatably fixed on the base, a force-applying mechanism is arranged on the top of the base, and a color sorter is arranged on the bracket.

[0017] Furthermore, it includes a base for containing the centrifuge, a shell arranged above the base forms a cavity, a rotating disk connected to the centrifuge is arranged inside the cavity, and a base is detachably arranged on the rotating disk through a fixed seat; an openable and closable upper cover is arranged above the shell, a connecting rod fixed on the side of the upper cover is movably connected to a lifting rod fixed on the base through a hinge joint, and a locking buckle is arranged on the front side of the upper cover; a display screen and control buttons are arranged on the front side of the base.

[0018] Furthermore, the structure on the bracket for fixing the sterile syringe is divided into two parts, namely an upper tube buckle and a lower tube buckle; the upper tube buckle has a coarse diameter and buckles the tube body of the sterile syringe, and the lower tube buckle has a fine diameter and buckles the injection port of the sterile syringe.

[0019] Furthermore, the upper tube buckle is a C-shaped structure, and both ends of the opening C are additionally provided with cylindrical clamping openings for fixing and clamping with the sterile syringe body.

[0020] Furthermore, the power plug and signal connector arranged at the bottom of the base are integrated together, and the bottom of the base remains flat; the color sorter on the bracket is connected to the base through a wiring harness; optionally, the color sorter can be arranged on the base, and only a signal acquisition head is arranged at the corresponding injection port of the sterile syringe on the bracket.

[0021] The present invention also discloses a method for extracting and separating PRP using the PRP automatic separator, comprising the following steps:

[0022] (1) Sample preparation: Use a sterile syringe (C) to draw the whole blood sample to be centrifuged;

[0023] (2) System assembly: connect the sterile syringe after blood extraction (1) and two other blank sterile syringes (A and B) of the same model as in (1) through three-way pipes; the three syringes are arranged horizontally, and the arrangement order from right to left is: (1) the syringe after blood extraction, the blank sterile syringe (B), the blank sterile syringe (A); and the left side of the leftmost three-way pipe and the right side of the rightmost three-way pipe are closed, and the three-way switch is turned on to ensure that the three syringes are connected;

[0024] (3) System installation: Open the bracket, install the three syringes assembled in (2) side by side on the bracket, and fix the bracket on the base; at the same time, pull down the push rod head above the base, and fix the push handle of the sterile syringe to the push rod head without pushing or pulling the piston column of the sterile syringe;

[0025] (4) Preparation for start-up: Install the base in (3) onto the rotating disk of the centrifuge and fix it. After checking that everything is correct, cover the upper cover and seal it, and prepare for start-up;

[0026] (5) Start the first centrifugation: The first centrifugation operation is performed at a speed of 120 g to 180 g for 10 to 25 min. After the centrifugation is completed, the color sorter below the syringe performs real-time color sorting and comparison, and then the leftmost blank syringe is started to pull the push rod head to extract at a uniform speed. At this time, the push rod head of the middle blank syringe remains stationary, and the dark red red blood cell layer material in the rightmost syringe enters the left syringe under the pull of the left syringe, and the push rod head of the right syringe follows;

[0027] When the color sorter on the right detects that the dark red color disappears, the plunger head of the syringe on the right remains stationary, the plunger head of the blank syringe in the middle remains stationary, and the left side stops after the extraction is completed;

[0028] (6) Starting the second centrifugation: The second centrifugation operation has a centrifugal speed of 240 g to 500 g and a duration of 10 to 25 min;

[0029] After centrifugation is completed, the color sorter below the syringe performs real-time color sorting and comparison. After the right color sorter detects the dark red substance, continue the operation in (5); when white or slightly yellowish color is detected, the plunger head of the left syringe remains stationary, and the plunger head of the middle syringe draws at a uniform speed. The white or slightly yellowish substance in the rightmost syringe enters the middle syringe under the pull of the left syringe, and the right syringe follows.

[0030] (7) Multiple centrifugation: As needed, the system starts the centrifugation operation again, with a centrifugal speed of 240g-400g and a duration of 10-25min. The color sorters under the three syringes perform color sorting and comparison at the same time, and the dark red material is drawn into the left syringe, and the white or slightly yellowish material is drawn into the middle syringe.

[0031] Only a single syringe was drawn at a time, that is, when the left syringe was drawn or pulled or pressed, either the middle syringe remained stationary or the right syringe remained stationary;

[0032] When the middle syringe is drawn or pulled or pressed, either the left syringe or the right syringe remains stationary;

[0033] (8) Completion of centrifugation: After centrifugation, the left syringe mainly contains red blood cell layer material, the middle syringe mainly contains PRP layer (white or slightly yellowish material, PRP and a small amount of plasma layer), which is the required material, and the right syringe contains the residual plasma layer.

[0034] Furthermore, steps (5), (6) and (7) can be completed manually according to the prompts on the display screen, or can be completed automatically by program setting; when completed automatically, the color sorters under the three syringes simultaneously perform color sorting and comparison, and the dark red substance is drawn into the left syringe, and the white or slightly yellowish substance is drawn into the middle syringe; and only a single syringe is drawn each time, that is, when the left syringe is drawn or pulled or pressed, either the middle syringe remains stationary, or the right syringe remains stationary; after the centrifuge is started for a duration of 10-25 minutes, it is left to stand for 10-60 seconds, waiting for the sample to stand for stratified color sorting and identification.

[0035] Furthermore, after the first centrifugation, the color sorter under the sterile syringe on the right side identifies and reserves more red blood cells, which are left for extraction into the sterile syringe on the left side after the next centrifugation, so as to avoid the sterile syringe on the left side extracting too much mixed liquid at one time and avoiding the extraction of PRP.

[0036] Beneficial effects of the invention: The invention provides a PRP automatic separator and an extraction and separation method thereof, provides a PRP automatic separator and an extraction and separation method thereof, does not require multiple times of taking and placing samples, and sampling is performed by automatic machine recognition, effectively solving the problem of contact with the outside world during transfer and centrifugation, reducing the risk of contamination during the PRP preparation process, and at the same time facilitating extraction, improving purity, and rejecting residual contamination.

[0037] (1) There is only one centrifugation process, and there is no need to install it again for secondary sampling after separation, which avoids the open preparation system being easily contaminated by the outside world, improves efficiency and reduces the risk of contamination.

[0038] (2) Use a color sorter for screening to avoid manual color discrimination, which reduces the difficulty of operation and also reduces the use of separation reagents during blood cell separation, reducing the residual separation agent (red blood cells, etc.);

[0039] (3) Even if PRP with high purity is prepared by multiple centrifugation, only one loading and unloading is required, and the possibility of secondary contamination is reduced to a minimum;

[0040] (4) There is no manual visual judgment and extraction of useful PRP substances, which requires high technical skills from the operator, is prone to errors, and has different effects. Sampling mainly depends on machine recognition and force-applying mechanisms. Moreover, it can be separated manually by pressing buttons according to the prompts on the display screen, or it can be automatically completed by program settings. Separation is more flexible. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 This is a schematic diagram of secondary centrifugation in the existing PRP preparation process.

[0042] Figure 2 Schematic diagram of plasma obtained after the first centrifugation in an existing sterile syringe.

[0043] Figure 3 It is a schematic diagram of the three-dimensional structure of the PRP automatic separator of the present invention.

[0044] Figure 4 This is a schematic diagram of the PRP automatic separator in the open cover state of the present invention.

[0045] Figure 5 This is a schematic diagram of the disassembled structure of the PRP automatic separator of the present invention.

[0046] Figure 6 It is a schematic diagram of the fixing assembly of the PRP automatic separator of the present invention.

[0047] Figure 7 It is a schematic diagram of the open state of the fixing assembly of the PRP automatic separator of the present invention.

[0048] Figure 8 This is a side view of the PRP automatic separator fixing assembly of the present invention in an opened state.

[0049] Fig. 9 The present invention discloses a PRP automatic separator fixing component bracket.

[0050] Fig.10 This is a rear view of the fixing component bracket of the PRP automatic separator of the present invention.

[0051] Fig.11 The invention discloses a three-aseptic syringe assembly system for the PRP automatic separator.

[0052] Fig.12 After the debugging of the three sterile syringe assembly systems of the PRP automatic separator of the present invention is completed.

[0053] Fig.13 The present invention is an assembly of a sterile syringe and a three-way connection of the PRP automatic separator.

[0054] Fig.14 It is a schematic diagram of the control circuit of the PRP automatic separator of the present invention.

[0055] The text labels in the figure are as follows: 1. base; 2. display screen; 3. button; 4. shell; 41. cavity; 5. lifting rod; 51. hinge joint; 52. connector; 6. upper cover; 61. locking buckle; 7. bracket; 71. upper tube buckle; 72. lower tube buckle; 711. buckling column; 73. rotating shaft; 74. color sorting port; 8. base; 81. bottom of base; 82. top seat; 83. rotating hole; 9. telescopic motor; 91. push rod head; 10. color sorter; 11. rotating disk; 1101. fixed seat; 50. sterile syringe; 501. piston column; 502. push handle; 503. injection port; 60. three-way pipe; 601. threaded interface; 602. screw cap; 603. rotary switch; 70. end seal one; 80. end seal two. DETAILED DESCRIPTION

[0056] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention is described in detail below in conjunction with the accompanying drawings. The description in this part is only exemplary and explanatory and should not have any limiting effect on the protection scope of the present invention.

[0057] Figure 1 This is a schematic diagram of secondary centrifugation in the existing PRP preparation process. Figure 2 This is a schematic diagram of plasma obtained after the first centrifugation of an existing sterile syringe. This is to facilitate understanding of the use of secondary centrifugation to obtain PRP and the phenomenon of blood centrifugation stratification.

[0058] Example 1: See Figure 3-14, is an embodiment of the present invention, and there are other possible similar structures. The structural description here does not constitute a limitation on the claims of this application.

[0059] A PRP automatic separator includes a sterile syringe 50 for sampling, a centrifuge for providing centrifugal action (not shown in the figure, connected to a rotating disk 11 and located below the rotating disk 11), a fixing assembly used in conjunction with the centrifuge, and a separator for purifying the sample of the sterile syringe 50 after centrifugation; the fixing assembly includes: a detachable base 8, and a force-applying mechanism arranged above the base 8 for pushing and pulling the piston rod 501 of the sterile syringe 50, a bracket 7 fixed on the base 8 for fixing the sterile syringe 50 to be centrifuged, and a color sorter 10 for dynamically color sorting the injection port 503 of the sterile syringe 50; the base 8 is fixed by a fixing seat 1101 arranged on the rotating disk 11; the separator is a three-way pipe 60, one end of which has an end seal when in use, see Fig.12 When used in combination, the two ends are sealed at end 1 and end 2. The other two ends are connected to the sterile syringe 50 after centrifugation and the sterile syringe for extracting operation; in this embodiment, by Figure 11-13 The structure is presented in the form of .

[0060] The base 8 is provided with a power plug and a signal connector at the bottom thereof, and they correspond to the sockets provided on the rotating disk; a control circuit board CPU, a centrifuge, and a mains power interface are provided inside the separator, and the circuit board CPU is electrically connected to the centrifuge, the base, and the force-applying mechanism and the color sorter on the base.

[0061] See also Fig.13 ,as well as Figure 11-Figure 12 Preferably, the sterile syringes 50 are three syringes with threaded injection ports of the same model, which are connected in series in parallel through three separators; the one at the right end is a sampling sterile syringe, and the other two are blank syringes, and the original state of the blank syringes is that the piston column is in the lowest position.

[0062] Preferably, the fixed component is a C-shaped structure, and the fixed component includes an external base 8 and a bracket 7 rotatably fixed on the base, a force applying mechanism is arranged on the top of the base, and a color sorter 10 is arranged on the bracket 7.

[0063] See also Figure 3-Figure 5Preferably, it includes a base 1 for containing the centrifuge, a shell arranged above the base forms a cavity 41, a rotating disk 11 connected to the centrifuge is arranged inside the cavity 41, and the rotating disk 11 is detachably provided with the base 1 through a fixed seat 1101; an openable and closable upper cover 6 is arranged above the shell 4, a connecting rod 52 fixed on the side of the upper cover 6 is movably connected with a lifting rod 5 fixed on the base 1 through a hinge joint 51, and a locking buckle 61 is arranged on the front side of the upper cover 6; a display screen 2 and buttons 3 for operation control are arranged on the front side of the base 1.

[0064] See also Figure 6-Figure 9 Preferably, the structure on the bracket 7 for fixing the sterile syringe 50 is divided into two parts, namely an upper tube buckle 71 and a lower tube buckle 72; the upper tube buckle 71 has a coarse diameter and buckles the tube body of the sterile syringe 50, and the lower tube buckle 72 has a fine diameter and buckles the injection port 503 of the sterile syringe 50.

[0065] Preferably, the upper tube buckle 71 is a C-shaped structure, and both ends of the opening C are additionally provided with cylindrical clamping openings for fixing and clamping with the sterile syringe body 50, and its structure is a cylindrical clamping column 711, see Figure 7 .

[0066] Preferably, the power plug and signal connector arranged at the bottom of the base 1 are integrated together, and the bottom of the base 1 remains flat; the color sorter 10 on the bracket 7 is connected to the base 1 through a wiring harness; optionally, the color sorter 10 can be arranged on the base 1, and only a signal acquisition head is arranged corresponding to the injection port 50 of the sterile syringe on the bracket 7.

[0067] A color selection port 74 is provided on the support 7 at a position corresponding to the injection port 503 of the sterile syringe 50. The signal acquisition of the color sorter 10 performs real-time signal acquisition and comparison of the sterile syringe 50 through the vacancy.

[0068] In addition, the rotation of the bracket 7 is achieved by the rotation shaft 73 provided on the side of the bracket 7 and the rotation hole 83 on the base bottom 81 of the base 8, so as to achieve rotation and positioning clamping. The internal structure can adopt a structure similar to a ratchet, or other simple and easy clamping methods, as long as the bracket 7 can be rotated and fixed on the base 8.

[0069] See also Fig.13 In this example, the injection port 503 at the lower end of the sterile syringe 50 has a thread. When it is connected to the three-way pipe 60, it is threadedly connected with the threaded interface 601 on the three-way pipe 60 to achieve pipeline sealing; at the same time, the three-way pipes 60 are threadedly connected and sealed by the screw caps 602. In addition, the rotation switch 603 of the three-way pipe 60 can be rotated without disassembling the sample when necessary, and differentiated sealing is performed for special centrifugal requirements.

[0070] Example 2: The present invention also discloses a method for extracting and separating PRP using the PRP automatic separator, comprising the following steps:

[0071] (1) Sample preparation: Use a sterile syringe (C) to draw the whole blood sample to be centrifuged;

[0072] (2) System assembly: Connect the sterile syringe used to draw blood in (1) and two other sterile blank syringes (A and B) of the same model as in (1) through three-way pipes; arrange the three syringes horizontally, and the order from right to left is: (1) the syringe used to draw blood, the sterile blank syringe (B), the sterile blank syringe (A); and the left side of the leftmost three-way pipe and the right side of the rightmost three-way pipe are sealed ( Fig.12 When the three-way tube 60 of the right sterile syringe 50 is selected to the 60' position, the right side is closed. ), and the three-way switch is turned on to ensure that the three syringes are connected;

[0073] (3) System installation: Open the bracket, install the three syringes assembled in (2) side by side on the bracket, and fix the bracket on the base; at the same time, pull down the push rod head above the base, and fix the push handle 502 of the sterile syringe 50 to the push rod head 91 without pushing or pulling the piston column 501 of the sterile syringe 50;

[0074] (4) Preparation for start-up: Install the base in (3) onto the rotating disk of the centrifuge and fix it. After checking that everything is correct, cover the upper cover and seal it, and prepare for start-up;

[0075] (5) Start the first centrifugation: The first centrifugation operation is performed at a speed of 120 g to 180 g for 10 to 25 min. After the centrifugation is completed, the color sorter below the syringe performs real-time color sorting and comparison, and then the leftmost blank syringe is started to pull the push rod head to extract at a uniform speed. At this time, the push rod head of the middle blank syringe remains stationary, and the dark red red blood cell layer material in the rightmost syringe enters the left syringe under the pull of the left syringe, and the push rod head of the right syringe follows;

[0076] When the color sorter on the right detects that the dark red color disappears, the plunger head of the syringe on the right remains stationary, the plunger head of the blank syringe in the middle remains stationary, and the left side stops after the extraction is completed;

[0077] (6) Starting the second centrifugation: The second centrifugation operation has a centrifugal speed of 240 g to 500 g and a duration of 10 to 25 min;

[0078] After centrifugation is completed, the color sorter below the syringe performs real-time color sorting and comparison. After the right color sorter detects the dark red substance, continue the operation in (5); when white or slightly yellowish color is detected, the plunger head of the left syringe remains stationary, and the plunger head of the middle syringe draws at a uniform speed. The white or slightly yellowish substance in the rightmost syringe enters the middle syringe under the pull of the left syringe, and the right syringe follows.

[0079] (7) Multiple centrifugation: As needed, the system starts the centrifugation operation again, with a centrifugal speed of 240g-400g and a duration of 10-25min. The color sorters under the three syringes perform color sorting and comparison at the same time, and the dark red material is drawn into the left syringe, and the white or slightly yellowish material is drawn into the middle syringe.

[0080] Only a single syringe was drawn at a time, that is, when the left syringe was drawn or pulled or pressed, either the middle syringe remained stationary or the right syringe remained stationary;

[0081] When the middle syringe is drawn or pulled or pressed, either the left syringe or the right syringe remains stationary;

[0082] (8) Completion of centrifugation: After centrifugation, the left syringe mainly contains red blood cell layer material, the middle syringe mainly contains PRP layer (white or slightly yellowish material, PRP and a small amount of plasma layer), which is the required material, and the right syringe contains the residual plasma layer.

[0083] Preferably, steps (5), (6) and (7) can be completed manually according to the prompts on the display screen, or can be completed automatically by program setting; when completed automatically, the color sorters under the three syringes simultaneously perform color sorting and comparison, and the dark red substance is drawn into the left syringe, and the white or slightly yellowish substance is drawn into the middle syringe; and only a single syringe is drawn each time, that is, when the left syringe is drawn or pulled or pressed, either the middle syringe remains stationary, or the right syringe remains stationary; after the centrifuge is started for a duration of 10-25 minutes, it is left to stand for 10-60 seconds, waiting for the sample to stand for stratified color sorting and identification.

[0084] Preferably, after the first centrifugation, the color sorter under the sterile syringe on the right identifies and reserves more red blood cells, which are left for extraction into the sterile syringe on the left after the next centrifugation, to avoid the sterile syringe on the left extracting too much mixed liquid at one time and to avoid extracting PRP.

[0085] It should be noted that, in this article, the terms "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or apparatus that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or apparatus.

[0086] This article uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only used to help understand the method and core ideas of the present invention. The above is only a preferred implementation of the present invention. It should be pointed out that due to the limitations of textual expression and the objective existence of infinite specific structures, ordinary technicians in this technical field can make several improvements, modifications or changes without departing from the principles of the present invention, and can also combine the above technical features in an appropriate manner; these improvements, modifications, changes or combinations, or the direct application of the inventive concept and technical solution to other occasions without improvement, should be regarded as the protection scope of the present invention.

Claims

1. A PRP automatic separator, characterized in that: It includes a sterile syringe for sampling, a centrifuge for providing centrifugal action, a fixed assembly used in conjunction with the centrifuge, and a separator for purifying the sample from the sterile syringe after centrifugation; The fixing assembly includes: a detachable base, and a force-applying mechanism arranged above the base for pushing and pulling the piston rod of the sterile syringe, a bracket fixed on the base for fixing the sterile syringe to be centrifuged, and a color sorter for dynamically color sorting the injection port of the sterile syringe; the base is fixed by a fixing seat arranged on the rotating disk; The separator is a three-way tube, one end of which is sealed when in use, and the other two ends are respectively connected to the sterile syringe after centrifugation and the sterile syringe for extraction operation; The base bottom of the base is provided with a power plug and a signal connector, which correspond to the socket provided on the rotating disk; the separator is provided with a control circuit board CPU, a centrifuge, and a mains power interface, and the circuit board CPU is electrically connected to the centrifuge, the base, and the force-applying mechanism and the color sorter on the base.

2. A PRP automatic separator according to claim 1, characterized in that: The sterile syringes are three syringes with threaded injection ports of the same model, which are connected in series in parallel through three separators; the one at the right end is a sampling sterile syringe, and the other two are blank syringes, and the original state of the blank syringes is that the piston rod is at the lowest position.

3. A PRP automatic separator according to claim 1, characterized in that: The fixed component is a C-shaped structure, and includes an external base and a bracket rotatably fixed on the base. A force-applying mechanism is arranged on the top of the base, and a color sorter is arranged on the bracket.

4. A PRP automatic separator according to any one of claims 1 to 3, characterized in that: It includes a base for containing the centrifuge, a shell arranged above the base forms a cavity, a rotating disk connected to the centrifuge is arranged inside the cavity, and a base is detachably arranged on the rotating disk through a fixed seat; An openable upper cover is arranged above the shell, a connecting rod fixed on the upper side of the upper cover is movably connected with a lifting rod fixed on the base through a hinge joint, and a locking buckle is arranged on the front side of the upper cover; A display screen and control buttons are arranged on the front side of the base.

5. A PRP automatic separator according to any one of claims 1 to 3, characterized in that: The structure on the bracket for fixing the sterile syringe is divided into two parts, namely an upper tube buckle and a lower tube buckle; the upper tube buckle has a coarse diameter and buckles the tube body of the sterile syringe, and the lower tube buckle has a fine diameter and buckles the injection port of the sterile syringe.

6. A PRP automatic separator according to claim 5, characterized in that: The upper tube buckle is a C-shaped structure, and both ends of the opening C are additionally provided with cylindrical clamping openings for fixing and clamping with the sterile syringe body.

7. A PRP automatic separator according to any one of claims 1 to 3, characterized in that: The power plug and signal connector arranged at the bottom of the base are integrated together, and the bottom of the base remains flat; the color sorter on the bracket is connected to the base through a wiring harness; optionally, the color sorter can be arranged on the base, and only a signal acquisition head is arranged at the corresponding injection port of the sterile syringe on the bracket.

8. A method for extracting and separating PRP using the PRP automatic separator as in claim 1-7, characterized in that: The following steps are involved: (1) Sample preparation: Use a sterile syringe (C) to draw the whole blood sample to be centrifuged; (2) System assembly: connect the sterile syringe after blood extraction (1) and two other blank sterile syringes (A and B) of the same model as in (1) through three-way pipes; the three syringes are arranged horizontally, and the arrangement order from right to left is: (1) the syringe after blood extraction, the blank sterile syringe (B), the blank sterile syringe (A); and the left side of the leftmost three-way pipe and the right side of the rightmost three-way pipe are closed, and the three-way switch is turned on to ensure that the three syringes are connected; (3) System installation: Open the bracket, install the three syringes assembled in (2) side by side on the bracket, and fix the bracket on the base; at the same time, pull down the push rod head above the base, and fix the push handle of the sterile syringe to the push rod head without pushing or pulling the piston column of the sterile syringe; (4) Preparation for startup: Install the base in (3) onto the rotating disk of the centrifuge and fix it. After checking that everything is correct, cover the upper cover and seal it, and prepare for startup; (5) Starting the first centrifugation: The first centrifugation operation has a centrifugal speed of 120 g to 180 g and a duration of 10 to 25 min. After centrifugation, the color sorter under the syringe performs real-time color sorting and comparison, and then starts the leftmost blank syringe to pull the push rod head to extract at a uniform speed. At this time, the push rod head of the middle blank syringe remains stationary, and the dark red red blood cell layer material in the rightmost syringe enters the left syringe under the pull of the left syringe, and the push rod head of the right syringe follows; When the color sorter on the right detects that the dark red color disappears, the plunger head of the syringe on the right remains stationary, the plunger head of the blank syringe in the middle remains stationary, and the left side stops after the extraction is completed; (6) Starting the second centrifugation: The second centrifugation operation has a centrifugal speed of 240 g to 500 g and a duration of 10 to 25 min; After centrifugation is completed, the color sorter below the syringe performs real-time color sorting and comparison. After the right color sorter detects the dark red substance, continue the operation in (5); when white or slightly yellowish color is detected, the plunger head of the left syringe remains stationary, and the plunger head of the middle syringe draws at a uniform speed. The white or slightly yellowish substance in the rightmost syringe enters the middle syringe under the pull of the left syringe, and the right syringe follows. (7) Multiple centrifugation: As needed, the system starts the centrifugation operation again, with a centrifugal speed of 240g-400g and a duration of 10-25min. The color sorters under the three syringes perform color sorting and comparison at the same time, and the dark red material is drawn into the left syringe, and the white or slightly yellowish material is drawn into the middle syringe. Only a single syringe was drawn at a time, that is, when the left syringe was drawn or pulled or pressed, either the middle syringe remained stationary or the right syringe remained stationary; When the middle syringe is drawn or pulled or pressed, either the left syringe or the right syringe remains stationary; (8) Completion of centrifugation: After centrifugation, the left syringe mainly contains red blood cell layer material, the middle syringe mainly contains PRP layer (white or slightly yellowish material, PRP and a small amount of plasma layer), which is the required material, and the right syringe contains the residual plasma layer.

9. The PRP extraction and separation method using the PRP automatic separator according to claim 8, characterized in that: Steps (5), (6), and (7) can be completed manually according to the display screen prompts, or they can be completed automatically by program settings; When the process is completed automatically, the color sorters under the three syringes will perform color sorting and comparison at the same time. The dark red substance will be drawn into the left syringe, and the white or slightly yellowish substance will be drawn into the middle syringe. Only a single syringe is drawn at a time, that is, when the left syringe is drawn or pulled or pressed, either the middle syringe remains stationary or the right syringe remains stationary; After the centrifuge is started for 10-25 minutes, it is allowed to stand for 10-60 seconds, waiting for the samples to be separated and identified by color sorting.

10. The PRP extraction and separation method using the PRP automatic separator according to claim 9, characterized in that: After the first centrifugation, the color sorter under the sterile syringe on the right identifies and reserves more red blood cells, which will be drawn into the sterile syringe on the left after the next centrifugation to prevent the sterile syringe on the left from drawing too much mixed liquid at one time and avoiding drawing away PRP.

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